107 results about "Gram negative bacterium" patented technology

The invention discloses a lactobacillus rhamnosus capable of improving joint inflammation and promoting bone health, a metagen and application of the lactobacillus rhamnosus, the lactobacillus rhamnosus is lactobacillus rhamnosus BN02, the lactobacillus rhamnosus is preserved in China General Microbiological Culture Collection Center on November 27, 2023, and the preservation number is CGMCC No.29133. The lactobacillus rhamnosus can be used for improving joint inflammation and promoting bone health. The lactobacillus rhamnosus BN02 can reduce the number of gram-negative bacteria in intestinal tracts of arthritis patients, increase the synthesis amount of bone collagen, and is beneficial to promoting absorption of vitamin C by the intestinal tracts, improving joint inflammation and promoting bone health.

An antibacterial polymer having a molecular weight above a certain level, which is highly miscible with other polymers compared to a monomolecular type antibacterial agent and can be added during the melt extrusion process. Further the antibacterial polymer has the advantage of exhibiting excellent antibacterial properties against Gram-negative bacteria and does not elute over time.

The invention discloses a bacteriophage lyase and application thereof, and particularly relates to a bacteriophage lyase with an amino acid sequence as shown in SEQ INNO.2. The bacteriophage lyase can inhibit the growth of escherichia coli, staphylococcus and salmonella, can be used as an antibacterial substance in splitting gram-negative bacteria and can be used for preparing drugs for resisting gram-negative bacteria. The lyase can be used independently or compounded with other substances, the use concentration of antibiotics can be reduced by combining the lyase with the antibiotics, toxic and side effects (such as renal toxicity of polymyxin) of drugs are reduced, and meanwhile, generation of bacterial drug resistance is delayed. After the lyase and the chitosan are combined for use, remarkable antibacterial activity is generated, the antibacterial effect can be achieved without pre-treatment on bacteria, and the practicability is improved.

The invention provides an antibacterial peptide and application thereof in resisting bacteria and / or enhancing the disease resistance of plants. Specifically, on one hand, the invention provides two antibacterial peptides which are novel functional antibacterial peptides ZJUAMP3 and ZJUAMP4, sequences of the novel functional antibacterial peptides ZJUAMP3 and ZJUAMP4 are respectively shown as SEQ ID NO: 1 and SEQ ID NO: 2, and the antibacterial peptides ZJUAMP3 and ZJUAMP4 both have broad-spectrum antibacterial activity, have good antibacterial activity on bacteria such as gram-negative bacteria, gram-positive bacteria and fungi, and are suitable for scenes needing sterilization or fungus and bacterium growth inhibition. Besides, the ZJUAMP4 can activate an MAPK signal channel in a plant body and induce the plant to generate systematic disease resistance, and is suitable for the fields of crop disease prevention and control and plant protection. The antibacterial peptide is simple in structure and easy to synthesize.

The invention discloses a preparation method and application of sodium gallate-polyphenol chitosan antibacterial hydrogel, firstly, gallic acid and sodium acetate are used as raw materials, a high-purity sodium gallate complex 5-carboxyl-2, 3-sodium dihydroxyphenolate is prepared at normal temperature and normal pressure, then a polyphenol compound and chitosan are cross-linked through Schiff base reaction, and the sodium gallate-polyphenol chitosan antibacterial hydrogel is obtained. And finally, carrying out rotary evaporation treatment, so that the sodium gallate is loaded in the polyphenol chitosan hydrogel system to obtain the antibacterial hydrogel. Sodium gallate is used as a functional component, uniform loading of sodium gallate in a polyphenol chitosan hydrogel system is realized through dual effects of physical adsorption and chemical bonding, the loading mechanism can effectively avoid aggregation and burst release of sodium gallate, stable release of antibacterial components is ensured, and the antibacterial property of the hydrogel is improved. The slow release time of the antibacterial hydrogel under the conditions that the pH values are 5.5 and 7.2 respectively exceeds 24 hours; and the antibacterial hydrogel has an inhibition effect on both gram-positive bacteria and gram-negative bacteria.

The present invention is predicated on success at producing a substance that results from removing LPS from a plant fermentation extract and the discovery that said substance demonstrates a biological activity that cannot be explained by conventional scientific common knowledge. The present invention is a method for obtaining an LPS-removed plant fermentation extract, an LPS-removed plant fermentation extract that is obtained using said method, and a formulation in which said LPS-removed plant fermentation extract has been compounded, said method being characterized by comprising a step in which LPS is removed from a plant fermentation extract that is obtained by fermenting a material derived from an edible plant, using a facultative anaerobic gram-negative bacterium that is exclusively symbiotic with the plant, and simultaneously cultivating said facultative anaerobic gram-negative bacterium.

The present application relates to the technical field of polysaccharide modification, and specifically discloses amino acid modified astragalus polysaccharide, a preparation method thereof and antibacterial application. The present application uses astragalus polysaccharide, formaldehyde and specific amino acids as raw materials, and prepares amino acid grafted modified astragalus polysaccharide through a Mannich reaction. The amino acid grafting site only occurs on the fifth carbon of the sugar ring. Not only does this retain the amino and carboxyl groups of the amino acid, so that the biological activity of the amino acid is retained, but also the active carboxyl group of the astragalus polysaccharide can be retained, so that the antibacterial property of the synthesized amino acid modified astragalus polysaccharide is significantly improved, and broad-spectrum antibacterial property can be achieved. The amino acid modified astragalus polysaccharide has excellent antibacterial activity on gram-positive bacteria and gram-negative bacteria, is a green new type of efficient antibacterial material, and is expected to become a substitute for antibiotics. The amino acid modified astragalus polysaccharide has important significance for solving the problems of increasing bacterial drug resistance and environmental accumulation caused by antibiotics, and has good application prospect.

The present invention belongs to the field of microbiology, and particularly relates to membrane vesicles (MVs) isolated from bacteria, and an isolation and preparation system and method for the membrane vesicles, and applications of the membrane vesicles. The bacteria of the present invention comprise Gram-positive bacteria and Gram-negative bacteria. The invention uses ionizing irradiation to irradiate bacteria, and isolates and purifies the produced membrane vesicles. The membrane vesicles prepared can be used as a vaccine, a vaccine adjuvant and / or a pharmaceutical carrier. In addition, the present invention provides a biological composition comprising the membrane vesicles and inactivated bacteria. In addition, the present invention also provides a preparation system, and isolation and purification system for bacterial membrane vesicles and the corresponding method. The membrane vesicles obtained by using the system and method have high yield, high purity and easy to be industrialized.

The application discloses a kind of implant and its preparation method and application, the implant includes material body, metal oxide layer and mixed crystal layer, the mixed crystal layer is located the surface of the metal oxide layer;The metal oxide layer includes metal oxide nanorod array;The metal oxide is selected from zinc oxide, silver oxide or its combination;The mixed crystal layer includes CaP crystal and metal oxide crystal.The implant of the application is sterilized by the bacteriostatic metal ion generated by physical puncture and degradation of metal oxide nanorod, has good antibacterial effect to gram-positive bacteria and gram-negative bacteria, prevents infection;It can also relieve post-implantation inflammatory response, reduce immune response under post-implantation infection, shorten wound healing time.In addition, due to the coating effect of mixed crystal layer on metal oxide layer, the bacteriostatic metal ion is slowly released, the release time is long, and long-lasting antibacterial effect can be achieved, and post-implantation inflammatory response can be reduced.

The present application relates to the technical field of medical bandage, and discloses a biomass antibacterial hemostatic bandage and a functionalization method thereof.The bandage comprises a bandage base material and a composite antibacterial functional layer loaded thereon, the functional layer is composed of a composite antibacterial unit and lysozyme, and the composite antibacterial unit comprises a cationic antibacterial core, a polyphenol sustained-release shell and an enzymatic cellulose scaffold.The present application forms a biomass antibacterial hemostatic bandage with broad-spectrum, long-acting, low drug resistance and hemostatic function by constructing a cationic antibacterial core-polyphenol sustained-release shell-enzymatic cellulose scaffold composite antibacterial unit and synergistically loading lysozyme.The bandage shows significant technical advantages in dealing with complex wound infection, especially gram-negative bacterial infection and drug-resistant bacterial challenge, and provides an efficient and safe solution for clinical wound management, and has important clinical application value and broad market prospect.

An effective treatment method for a broad variety of diseases in both animals and humans is disclosed. The method includes combining one or more vaccines with a treatment compound to enhance vaccine efficacy. The disclosed treatment compound does not act directly on the pathogen, and thus the organisms cannot readily develop resistance to the treatment. When a compound such as, but not limited to, the disclosed compound is used in conjunction with one or more vaccines, a synergistic effect is realized. The suggested compound is derived from a lipopolysaccharide (LPS) of gram negative bacteria. The treatment compound is combined with one or more appropriate vaccines and is administered early in the life of an animal to achieve a synergistic effect compared with the use of the treatment compound or the vaccine alone.

The invention discloses application of bacteriophage lyase LysFEC14 in preparation of a composition for resisting gram-negative bacteria. The amino acid sequence of the bacteriophage lyase LysFEC14 is as shown in SEQ ID NO.01. The invention further discloses a preparation method of the bacteriophage lyase LysFEC14. The antibacterial composition disclosed by the invention shows high-efficiency cracking activity on various escherichia coli standard strains, still has a remarkable antibacterial effect at low concentration, and provides a green and efficient novel antibacterial composition for preventing and treating gram-negative bacterium infection, particularly drug-resistant escherichia coli infection.

The invention discloses a carbapenem drug resistance marker screening method and system based on a cross-species compressed Debrueine diagram and a medium. The method comprises the following steps: starting from whole genome sequencing data of gram-negative bacteria belonging to different species and carbapenem drug phenotypes of the gram-negative bacteria, constructing a compressed Debrueine graph based on cross-species joint data, and taking existence / deletion of nodes in the graph as unified genetic variation characteristics. Performing correlation analysis on the nodes and the drug resistance phenotypes by using a linear hybrid model to obtain a candidate node set related to the phenotypes; k-mer is extracted based on the candidate node sequence, and secondary statistical screening is completed in combination with chi-square test and mutual information; and finally determining a group of carbapenem drug-resistant genetic markers which can be applicable across species through a hierarchical feature selection strategy of random forest and XGBoost. Efficient dimension reduction of large-scale cross-species genome data, cross-species consistent variation representation and high-interpretability marker screening are achieved.

This invention relates to an endogenous antimicrobial peptide from bovine milk and its applications. The antimicrobial polypeptide compound VAVALARPKHPIK is derived from endogenously enzymatically hydrolyzed bovine casein, with the amino acid sequence Val-Ala-Val-Ala-Leu-Ala-Arg-Pro-Lys-His-Pro-Ile-Lys. It exhibits broad inhibitory activity against both Gram-positive and Gram-negative bacteria, including *Porphyromonas gingivalis*, *Escherichia coli*, and *Staphylococcus aureus*. This antimicrobial peptide shows promising applications as a health supplement and / or medical device or pharmaceutical for the prevention and / or reduction of infectious diseases, as a food antimicrobial agent, in novel functional foods, pet food and animal feed additives, and in daily chemical products.

The present application relates to a kind of chlorella-nanose complex hydrogel and its preparation method and application, belong to nanose antibacterial technical field.The chlorella-nanose complex hydrogel includes hydrogel matrix, and the chlorella and photo-thermal nanose loaded on the hydrogel matrix.The present application also provides the preparation method of the complex hydrogel, and the application in the preparation of antibacterial drug or dressing and the killing gram-negative bacteria and gram-positive bacteria.The present application combines chlorella and nanose for the first time, and constructs a self-oxygen supply antibacterial platform.The chlorella in the chlorella-nanose complex hydrogel can carry out photosynthesis under illumination, continuously produce oxygen, provide sufficient substrate for nanose oxidase-like reaction, overcome the low activity defect of traditional nanose in anoxic environment.

The present invention relates to a method for treating Gram-negative bacteria that are resistant to colistin (MIC > 50 mg / mL), an antibacterial compound, excluding those of the genera Burkholderia, Proteus, and Serratia, comprising contacting the bacteria with an aqueous pharmaceutical composition containing a rose bengal (RB) compound of formula I, dissolved or dispersed at a concentration of about 0.01 to about 15 mg / mL, and irradiating the contacted bacteria with light of a wavelength of about 500 nm to about 600 nm for a period of about 1 to about 10 minutes, at a concentration of about 16 to about 160 J / cm². 2 The aim is to create a method that involves obtaining a certain amount of light to treat and kill irradiated bacteria. [Formula 1] JPEG2026511692000028.jpg5864 (where X, R 1 , R 2 (and M+ are defined herein)

The application belongs to the technical field of biological medicine, and relates to an antibacterial polypeptide, a composition and application thereof. The amino acid sequence of the antibacterial polypeptide is shown in SEQ ID NO. 1: KWKIKWPVKWFKM-NH2. Experiments show that the antibacterial polypeptide has broad-spectrum antibacterial activity on gram-positive bacteria and / or gram-negative bacteria, and simultaneously has low cytotoxicity and low hemolyticity. The antibacterial polypeptide provided in the application is composed of 14 amino acids, the polypeptide chain is short, the molecular weight is small, and the antibacterial polypeptide is easy to be artificially synthesized, is a small-molecule polypeptide with high application value, and can be used for preparing a novel antibacterial agent, and has wide application prospects.

The present application relates to the technical field of antibacterial technology, and particularly relates to a natural antibacterial agent and application thereof. The present application provides a method for preparing a composite antibacterial preparation by using Chinese medicine pomegranate peel and Bacillus subtilis AC-1 as raw materials. The composite antibacterial agent has the characteristics of strong resistance to gram-negative bacteria Escherichia coli, gram-negative bacteria Staphylococcus aureus, Staphylococcus pseudintermedius, pathogenic fungus Gymnoascus ruber, Microsporum canis and Microsporum gypseum.

The application discloses a CXCL-BPI fusion protein which can be used for treating gram-negative bacterial infection, a coding nucleic acid of the CXCL-BPI fusion protein, an expression preparation method, and application of the CXCL-BPI fusion protein to preparation of a pharmaceutical composition for treating gram-negative bacterial infection. The CXCL-BPI fusion protein comprises a human ELR+CXC chemokine and a human BPI N-terminal domain functional fragment, has the functions of combining LPS, directly killing gram-negative bacteria and chemotactic cell migration, and also has the functions of promoting phagocytes to be guided to combine and phagocytize gram-negative bacteria, and the action mechanism can overcome gram-negative bacterial drug resistance.

The application discloses a broad-spectrum bacteriostatic Clostridium subsp. and a preparation method and application thereof, and belongs to the technical field of microorganisms. The strain is named as DMS028, is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 38049. The strain has broad-spectrum bacteriostatic activity, can simultaneously inhibit gram-negative bacteria Aeromonas hydrophila in aquaculture, gram-positive bacteria Clostridium perfringens in livestock and poultry breeding, and plant diseases such as Exserohilum longipes and Fusarium oxysporum, and overcomes the defects of narrow bacteriostatic spectrum of existing microbial preparations. The preparation method adopts two-stage temperature anaerobic culture, and adds lysine methyl ester, leucine ethyl ester, palmitoyl glycine and lauroyl arginine ethyl ester as an antibacterial substance synergist and a stabilizer, and has the advantages of simple process, convenient operation, no need of complex equipment, easy scale production, efficient high-activity bacterial body and antibacterial product.

The invention discloses a small organic molecule photosensitizer for photodynamic sterilization and a preparation method and application thereof.The small organic molecule photosensitizer is obtained based on a triphenylamine structure through a Suzuki coupling reaction catalyzed by palladium in 1, 4-dioxane and a Knoevenagel reaction in acetonitrile, and reaction conditions needed by the synthesis method are mild and simple. The prepared photosensitizer has a wide range of absorption wavelength and near-infrared fluorescence emission band, and can sensitize oxygen into active oxygen under illumination. After the photosensitizer prepared by the invention is incubated with escherichia coli and pseudomonas aeruginosa together, the growth of the escherichia coli and the pseudomonas aeruginosa can be obviously inhibited; after the photosensitizer and the bacteria are incubated together and under the illumination of white light, the bacterial survival rates of the escherichia coli and the pseudomonas aeruginosa are obviously reduced, and the photodynamic sterilization application of the two gram-negative bacteria is successfully realized.

Endotoxin production inhibition in a water system, or both biofouling inhibition and endotoxin production inhibition can be achieved. In a membrane treatment method or a biofouling inhibition method, a bonded halogen compound is caused to exist in a water system where gram-negative bacteria exist. In an endotoxin production inhibition method in a water system, a bonded halogen compound is caused to exist in the water system.

The present invention provides a photopolymerisable composition for 4D printing, comprising an acrylated polyol (AOPO) derived from a natural oil, such as olive oil, and acrylic acid as a comonomer. The composition is used in a solvent-free digital light processing (DLR) method to fabricate high-resolution, multifunctional objects. The resulting 4D printed objects are biocompatible, hemocompatible, and cytocompatible. They exhibit excellent temperature- responsive shape memory properties activated near body temperature, inherent antimicrobial activity against both gram-negative and gram-positive bacteria, and tuneable mechanical properties suitable for soft tissue engineering. Furthermore, the composition can be loaded with therapeutic agents, such as progesterone, to form biodegradable objects capable of sustained, controlled drug release. These properties make the material ideal for medical implants, cardiovascular stents, and drug delivery devices.

This invention provides an intelligent computational method for predicting effector proteins in the secretory system of Gram-negative bacteria, comprising: S1: collection and preprocessing of protein sequence datasets; S2: model construction; S3: model training; and S4: model prediction. This invention has advantages such as high prediction accuracy and good anti-interference performance.

A pharmaceutical composition to be applied to an ocular surface is disclosed that includes a quaternized chitosan derivative to provide antimicrobial activity against gram-negative bacteria, gram-positive bacteria, fungi, and viruses as an active ingredient. This is combined with a cellulose compound acting as a viscosity agent and demulcent to protect and lubricate mucous membrane surfaces. This is further combined with a humectant and tonicity agent to retain moisture on the ocular surface and maintain a comfortable osmolarity for the eye. This is further combined with a buffer / pH adjuster system configured to support a physiologically tolerable pH of about 6.0 to 8.0. Finally, this is combined with purified water as a diluent.

The present application provides a composite sonosensitizer and its bacterial target delivery system BPT-ICG@Bd capable of treating pseudomonas aeruginosa biofilm related infection, wherein the composite sonosensitizer (BPT-ICG) is composed of inorganic sonosensitizer (BPT) and organic sonosensitizer (ICG), Bd is natural gram-negative bacteria predator Bdellovibrio, the composite sonosensitizer (BPT-ICG) is modified to the surface of Bdellovibrio (Bd) by polydopamine to form BPT-ICG@Bd. The high-speed movement characteristics of Bdellovibrio predator gram-negative bacteria are used to destroy the biofilm structure, invade bacteria, and at the same time, the composite sonosensitizer is triggered to produce a large amount of active oxygen by applying ultrasound, so as to quickly remove the biofilm and bacteria, and be used for biofilm related lung infection and bone infection.

The application discloses a long-acting antibacterial PP composite material for a cleaning brush and a preparation method, and relates to the technical field of antibacterial polypropylene. The long-acting antibacterial PP composite material for the cleaning brush is composed of the following components in mass parts: polypropylene resin 60-80 parts, polyisobutene 5-15 parts, inorganic filler 15-30 parts, antibacterial agent 0.5-2.0 parts, migration enhancer 3-8 parts, antioxidant 0.5-1.5 parts, lubricant 0.5-1.5 parts and nucleating agent 0.1-0.5 parts. The long-acting antibacterial PP composite material for the cleaning brush has excellent antibacterial performance. The antibacterial agent has a unique structure design, can efficiently destroy bacterial cell membranes, and thus realizes broad-spectrum antibacterial effect. Both gram-positive bacteria and gram-negative bacteria can be effectively inhibited, and the problem of insufficient antibacterial performance of traditional PP materials is solved.

The application provides an earthworm antibacterial peptide derived from a popular cavity earthworm and application thereof in broad-spectrum antibacterial aspects. The amino acid sequence of the antibacterial peptide is shown in SEQ ID NO. 1 or SEQ ID NO. 2, and both have 13 amino acid residues and isoelectric points of 10.31 or 10.06. The application adopts a bioinformatics method, finds a new earthworm antibacterial peptide through a machine learning algorithm, optimizes the amino acid sequence according to the prediction result, and finally obtains an antibacterial peptide with better antibacterial effect. The obtained antibacterial peptide has good antibacterial activity on gram-negative bacteria represented by escherichia coli and gram-positive bacteria represented by staphylococcus aureus, and various drug-resistant bacterial variants of the two kinds of bacteria, and can be applied to the preparation of antibacterial products, including antibacterial drugs, additives and preservatives and the like.

The invention relates to the field of bacterial membrane protein structures. More specifically, the invention relates to lipid nanodiscs compartmentalized by SlyB protein oligomers isolated from the outer membrane of Gram-negative bacteria. More specifically, the invention provides for a SlyB nanodisc structure wherein the SlyB-oligomer forms the membrane scaffold protein belt, which is surrounded by outer saccharolipid moieties anchored to the SlyB proteins, and which encloses a lipid bilayer nanodomain containing one or more phospholipid layers, wherein macromolecules such as (outer) membrane protein molecules may be captured and stabilized. More specifically, methods to produce and isolate chemically defined stable SlyB nanodisc particles are disclosed herein. Finally, the invention relates to the use of said SlyB nanodiscs as a self-adjuvanting vehicle, as part of an immunogenic composition, and provides for novel means for use in eliciting an immune response against macromolecules enclosed in said SlyB nanodiscs, or for use in a vaccine composition.

The invention discloses a cyclic peptide compound as well as a preparation method and application thereof. Specifically, the invention discloses a compound as shown in a formula (I), and a stereoisomer, a tautomer, a crystal form, a pharmaceutically acceptable salt, a hydrate, a solvate or a prodrug thereof. The compound provided by the invention is an antibiotic and can be used for preparing a medicine for treating and / or preventing diseases related to gram-negative bacteria.