559 results about "Gram negative bacterium" patented technology

The present invention relates to a disinfectant composition containing the following components (A) to (D): component (A): a zinc compound, component (B): a chelate compound represented by the following general formula (b1) or (b2), component (C): hydrogen peroxide or a peroxide that releases hydrogen peroxide in water, and component (D): an organic peroxy acid precursor that generates an organic peroxy acid by reacting with the component (C). According to the present invention, a disinfectant composition and a disinfecting method can be provided that enable the effective elimination of gram negative bacteria adhered to textile products, and particularly cotton products.

The invention relates pseudomonas, use thereof and a method for removing cadmium pollution to the environment. The pseudomonas class is named pseudomonas putidaHN103 and the collection number in a collection center is CCTCCNo.M2011184; and the shape and characteristics of the pseudomonas putidaHN103 comprise: 1) on a culture medium, a bacterial colony which used to be colorless and transparent turns white, smooth and convex-like with irregular edges; and 2) according to the result of bacterial microscopic examination, pseudomonas belongs to brevibacterium and is gram negative. The use of the bacteria is the use of the bacteria as a biological repair material for removing cadmium pollution. The method for removing cadmium pollution to the environment comprises the following steps: 1) regulating the cadmium ion concentration in sewage to a range in which pseudomonas can grow normally; 2) placing pseudomonas; 3) keeping the pseudomonas in the sewage for a certain time period; and 4) removing bacterial absorbing cadmium ions. The pseudomonas requires small investment, is low in cost, high in adaptability and capable of effectively removing cadmium, can quickly and obviously lower the concentration of cadmium ions in the environment and is suitable for biological remediation environments polluted by cadmium.

The invention relates to a natural preservative antistaling agent, which comprises epsilon-polylysine and alcohol extractions of anise, black plum, Cinnamomum cassia, and purple perilla, and can improve the cell membrane permeability of microorganism, thereby causing microorganism restricted substance leak, and can inhibit the growth of Gram-positive bacteria and Gram-negative bacterium, fungus, and saccharomycete in fresh-cut vegetables, and forming a layer of protective film on the surface of fruits and vegetables. The invention can prevent the intrusion of microbe, and reduces spoilage. When utilized in fresh-cut vegetables, the preservative antistaling agent can reduces the rotten rate and avoid the shortcoming that general chemical preservative needs lower PH, the invention is particularly suitable to the fresh-keeping of low acid foods such as vegetables, and can reduce loss in weight which is caused by water evaporation, and can be used for fresh-keeping of fresh-cut vegetables, inhibits oxidation and browning reaction and nutrient loss, prolongs the goods shelf life, and is a preservative antistaling agent which is safe and has no side effect. The invention can prolong the shelf life by 2-3 days.

Production of algin oligose and its usage are disclosed. The process is carried out by degrading for 1-5% algin solution at 40-80 degree and pH value 2-5 by supersonic wave for 7-10 hrs, adding anhydrous alcohol into degrading liquid, eluting by alcohol, extraction filtering, drying and grinding to obtain final product. The average polymerization is below 10 degree. It's simple and cheap, it can inhibit Gram-negative bacterium or positive bacterium growth effectively, have better broad-spectrum antibacterial activity, anti-inflammatory and analgetic functions.

The invention discloses a health food capable of improving intestinal floras and a preparation method of the health food. The intestinal floras are adjusted on a plurality of aspects of increasing the probiotics variety and quantity, adding probiotic nutrient substances, inhibiting the harmful bacterial growth and increasing the human immunity, so that the gastrointestinal functions are improved. With probiotic powder such as lactobacillus plantarum as a main raw material, an edible mushroom extract is scientifically compounded, and dietary fibers are modified, so that the human immunity can be significantly improved; a saussurea involucrate culture extract has the efficacies of resisting inflammation, relieving pain, and resisting oxidation, radiation and fatigue; the variety of the intestinal beneficial bacteria floras is increased; the field planting capacity of endogenous and exogenous probiotics in human intestinal tracts is significantly enhanced and the field planting time of endogenous and exogenous probiotics in human intestinal tracts is significantly prolonged; the growth and the reproduction of intestinal harmful bacteria, especially gram negative bacteria, are effectively inhibited; the components of the intestinal beneficial bacteria floras are fully adjusted; and the health food which is high in probiotic property, and is capable of significantly improving the human immunity and improving the intestinal floras is prepared.

The invention provides a new Lactobacillus plantarum bacterial strain YJG which was preserved in the China General Microbiological Culture Collection Center (CGNCC) with the collection number of CGMCC No. 2994 on march, 30th, 2009. The Lactobacillus plantarum bacterial strain YJG can produce broad-spectrum bacteriostatic activity bacteriocin with high yield, and can express bacteriostasis for staphylococcus aureus, salmonella and E.coli of gram-negative bacterium, L. plantarum, L.brevis, Streptococcus and Bacillus of gram-positive bacterium, and the like. A virus attacking tests with high dosage proves that the Lactobacillus plantarum bacterial strain YJG is safe to animal bodies. The Lactobacillus plantarum bacterial strain YJG and a zymophyte liquid thereof can be used for preparing additives of green animal feeds, and the bacteriocin of the Lactobacillus plantarum bacterial strain YJG can be used for preparing green biological veterinary drugs, environmental-friendly biopreservatives, biocontrol drugs and the like.

The present invention provides Cephem compounds which have a wide antimicrobial spectrum and have potent antimicrobial activity against beta-lactamase producing Gram negative bacteria as follows:

A compound of the formula:

wherein,

• • X is N, CH or C—Cl;
  • T is S or the like;
  • A and G are lower alkylene or the like;
  • B is a single bond or the like;
  • D is a single bond, —NR⁷—, —CO—, —CO—NR⁷—, —NR⁷—CO—, —NR⁷—CO—NR⁷—, or the like;
  • E is optionally substituted lower alkylene;
  • F is a single bond or optionally substituted phenylene;
  • R³, R⁴, R⁵ and R⁶ each is independently hydrogen, halogene, nitrile, or the like;

or an ester, a compound protected at the amino on the ring in the 7-side chain, a pharmaceutically acceptable salt, or a solvate thereof.

The invention relates to a bacterium S2 for efficiently generating biosurfactant and a fermentation culture medium thereof, belonging to the field of biotechnology. The bacterium S2 is gram-negative bacterium which is rod-shaped, is (6-8)*(8-12) micrometers in size and has no spores and capsules. The bacterial strain belongs to the pseudomonas aeruginosa through identifying by morphological and physio-biochemical characteristics and molecular biology, and the collection code is CGMCC No.3034. The best fermentation culture medium is a novel culture medium which takes 50g/L of rapeseed oil as the only carbon source. The rhamnolipid is the only fermentation product of the bacterial strain and has high yield. The CMC (Critical Micelle Concentration) value of the fermentation liquid is 0.25 g/L; compared with the common chemical surfactant, the bacterium has more obvious emulsification and durability on hydrophobic substances, such as oils and the like.

The invention discloses weight-losing probiotic tablets and a preparation method thereof. According to the weight-losing probiotic tablets and the preparation method thereof, probiotic powder such as lactobacillus plantarum is taken as a main raw material, and modified dietary fibres, fruit and vegetable powder, oligosaccharides, plant extract, protein powder, tea leaf extract, Chinese herb extracts and the like are scientifically compounded, thus increasing the content of soluble celluloses with real significance in probiotic groups, and enhancing the physiological activity of the celluloses, and then remarkably improving the planting capacity and the planting time of endogenous and exogenous probiotics in human intestinal tracts, effectively suppressing the growth and the reproduction of intestinal harmful bacteria, in particular, Gram negative bacteria, and adequately regulating the composition of the intestinal probiotic groups while increasing the types of the intestinal probiotic groups; the prepared weight-losing probiotic tablets are high in bioactivity, long in planting time in the human intestinal tracts, remarkable in weight-losing effect, and wide in the range of appropriate crowds.

Provided is a novel compound which is useful as a pharmaceutical composition by inhibiting an LpxC activity, thereby exhibiting potent antimicrobial activity against gram-negative bacteria including Pseudomonas aeruginosa and its drug resistant bacteria. Provided is a hydroxamic acid derivative represented by the following general formula [1] or a pharmaceutically acceptable salt thereof:

The invention relates to an acryloyl bis-quaternary ammonium salt monomer of a side chain with a reaction active group, the monomer has the structure general formula as is shown in the formula (1) at the right side, wherein, R1 represents alkyl having 1 to 20 carbon atoms or fluorine-containing alkyl; R2 represents a covalent bond or a NHCO(CH2)t group, the t in the group is an integer from 1 to 10; R3 represents hydrogen atoms or alkyl having 1 to 5 carbon atoms; n is an integer from 0 to 10; m is an integer from 0 to 10; k is an integer from 1 to 10; Y represents alkylamine having a structural formula (2) or sec-amido-NH- or oxygen atom, wherein, R4 is alkyl having 1 to 5 carbon atoms; X is chlorine or bromine atoms; and Z is a covalent bond or an amido bond or an ester bond. The invention also provides a preparation method of the monomer. The acryloyl bis-quaternary ammonium salt provided in the invention has excellent reaction activity, and can be easily guided into the polymer to make the acquired polymer have good sterilization function, in particular to gram-negative bacterium. The method is simple and convenient, and is high in yield, and the side reaction will not take place in the reaction process.

The invention discloses application of a substituted benzene guanidine derivative serving as a polymyxins antibiotic potentiator. For the first time, the substituted benzene guanidine derivative is found to be capable of serving as the polymyxins antibiotic potentiator, especially serving as a synergist of a polymyxins antibiotic to inhibit sensitive strains and a drug resistance reversal agent of the polymyxins antibiotic. The invention further provides combined application of the substituted benzene guanidine derivative or a combination containing the same and the polymyxins antibiotic in preparation of compound drug preparations for people or animals to treat diseases caused by gram-negative bacterial infection. The invention further provides combined application of the substituted benzene guanidine derivative or a combination containing the same and the polymyxins antibiotic in preparation of forage-use growth-promoting feed additives for animal breeding in animal husbandry.

A method for extracting astaxanthin, protein and chitin from shrimp shells through using microbes comprises following steps: utilizing gram negative bacterium adjacent pseudomonas bacillus (Plesiomonas) to degrade and ferment in the 37 DEG C condition, centrifuging and separating degradation products, concentrating and drying liquid, obtaining albumen powder, immersing solid body with dissolvent, centrifuging again, removing the dissolvent from the liquid, and obtaining the astaxanthin, wherein the medium-lower layer of precipitation is crude chitin and the medium-upper layer of the precipitation is cells and little protein which is used as bio-fertilizer. Compared with an existing technique, the invention has the prominent advantages that multi-purpose use is good, the microbes is applied to degrade and extract astaxanthin from the shrimp shells, the protein and the crude chitin are reclaimed, and the cells and the few protein can be used as the bio-fertilizer, which enables the shrimp shells to be utilized sufficiently and reaches the stage of 'eat and extract completely'. Simultaneously the invention has low pollution, low cost and good effect.

The invention relates to a borneol-based macromolecule antibacterial material. According to the macromolecule antibacterial material, four acrylyl-derivative borneol monomers of different chiral configurations are taken as raw materials, and macromolecules are generated by virtue of a polymerization reaction to be taken as the antibacterial material. A chiral macromolecule material has good anti-adhesive and growth-inhibiting effects on gram-positive bacterium, gram-negative bacterium and fungus, also has the characteristics of good biocompatibility and nontoxicity, can be taken as the antibacterial material applied to the fields of medicines, foods, health, and environmental protection and has a huge development potential.

Disclosed and claimed are a mutant of a gram negative bacterium, wherein said bacterium has at least one mutation in a nucleotide sequence which codes for a polypeptide having an identity which is equal or more than 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% with an amino acid sequence coded by a nucleotide sequence selected from the group consisting of nucleotide sequences identified SEQ ID NO: 2, 6, 9, 12, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 75, 78, 81, 84, 87, 90, 93; said mutation resulting in attenuated virulence of the bacterium. Immunogenic compositions and vaccines containing such a mutant are also disclosed and claimed.

Provided is a cephem compound which has a wide antimicrobial spectrum, and in particular exhibit potent antimicrobial activity against beta-lactamase producing Gram negative bacteria, and pharmaceutical composition comprising the same.

A Compound of the formula (I):

wherein

• • W and U are as defined in the specification;
  • R¹ is as defined in the specification;
  • R^2A and R^2B are as defined in the specification, provided that R^2A and R^2B are not taken together to form an optionally substituted oxime group when R¹ is aminothiazole or aminothiadiazole optionally protected at the amino group;
  • ring A is a benzene ring or a 6-membered aromatic heterocyclic group having 1-3 nitrogen atoms;
  • R³ is a hydrogen atom, —OCH₃ or —NH—CH(═O);
  • k is an integer from 0 to 2;
  • R⁴ is as defined in the specification; and
  • D and E are as defined in accordance with a) or b) described in the specification.

The invention discloses a magnetic molecular imprinting sensor for detecting a Gram-negative bacterium signal molecule, belonging to the technical field of rapid detection of pathogenic bacteria. The novel magnetic molecular imprinting sensor is constructed by combining a surface molecular imprinting technology, a magnetic separation technology and an electrochemical sensing technology; and meanwhile, the magnetic molecular imprinting sensor has the specificity of the molecular imprinting technology, the renewability and rapid separation property of the magnetic separation technology, and the rapidness and sensitivity of electrochemical analysis, so that low-content Gram-negative bacterium signal molecules can be accurately detected. The invention further discloses a preparation method and application of the magnetic molecular imprinting sensor; the indirect detection on partial Gram-negative bacteria can be realized; and the magnetic molecular imprinting sensor has the advantages of sensitivity, rapidness and high specificity and the like, is low cost and is suitable for detecting the Gram-negative bacteria in the fields of food industries, medical treatment industries and the like.

The invention discloses a quinclorac degrading bacteria and application thereof. The quinclorac degrading bacteria J3 belongs to Alcaligenes sp. and is collected in CGMCC (China General Microbiological Culture Collection Center); the collection date is March 28, 2013, the collection number is CGMCC No.7384, and the GenBank accession number of a bacterial strain 16S rDNA is KC693648. The quinclorac degrading bacteria is a Gram negative bacterium, and a bacterial colony has a smooth surface and a raised center, is in a faint yellow color and a circular shape and is regular on the edge and non-transparent. The quinclorac degrading bacteria disclosed by the invention has a quinclorac degradation function and can prevent the tobaccos from being injured by quinclorac.

The invention relates a preparation method of an inorganic-organic hybrid antibacterial agent with antibacterial and hydrophobic functions and application of the antibacterial agent in a polymer material matrix. The antibacterial agent overcomes the problem in the prior art that the antibacterial performance of a natural antibacterial agent cannot completely meet the demands of users when being used alone. Specifically, the antibacterial agent adopts an amino-containing coupling agent as a bridge, and antibacterial inorganic nanoparticles such as titanium dioxide and zinc oxide are coated withantibacterial aldehydes such as organic citral and cinnamaldehyde to form the inorganic-organic hybrid antibacterial agent. The inorganic-organic hybrid antibacterial agent can be widely applied to polymer materials such as PP and ABS and achieve an antibacterial and hydrophobic effect; the antibacterial rate of the antibacterial agent for gram-negative bacteria such as escherichia coli can reach99.9%, the antibacterial rate of the antibacterial agent for gram-positive bacteria such as staphylococcus aureus can reach 99.9%, the antibacterial rate of the antibacterial agent for aspergillus niger and other molds can reach 0 grade, and the contact angle between the material surface and water can reach 120 degrees. Meanwhile, the antibacterial polymer material maintains the great mechanicalproperty.

The invention provides a preparation method of a quaternary ammonium salinized fluorescent carbon dot and application of the quaternary ammonium salinized fluorescent carbon dot in bacterium resisting and gram positive bacterium and gram negative bacterium differentiating. The preparation method of the quaternary ammonium salinized fluorescent carbon dot mainly comprises the two steps that a surface-aminated carbon dot is prepared, and then alkyl betaine is grafted to the surface of the aminated carbon dot to obtain the quaternary ammonium salinized carbon dot. The prepared quaternary ammonium salinized carbon dot has the good water solution dispersibility and the low cytotoxicity and can effectively inhibit and kill gram positive bacteria. In addition, the quaternary ammonium salinized carbon dot has the good fluorescent property and can selectively enable the gram positive bacteria to be imaged to effectively differentiate the gram positive bacteria and gram negative bacteria.

The invention relates to burkholderia pyrrocinia and application thereof, which belong to the field of environmental microorganism application. The burkholderia pyrrocinia B1213 provided by the invention has the preservation No. CGMCC No.12806, is preserved on July 21, 2016, belongs to a bacilliform gram-negative bacterium, and cannot form gemma. The burkholderia pyrrocinia B1213 provided by the invention can degrade dibutyl phthalate (DBP); the degradation rate of the DBP can reach up to 45.5 to 75.35 percent. A method for adopting the burkholderia pyrrocinia B1213 provided by the invention to degrade the DBP is characterized in that in the presence of a yeast extract, the burkholderia pyrrocinia B1213 is contacted with DBP. Compared with a method for adopting bacteria and fungus to degrade the DBP, the method for degrading the DBP provided by the invention is novel in strain source, high in degradation rate, short in degradation period, and simple to operate.

The invention provides a breast perfusion preparation for treating mammitis of milk cow in lactation period, which belongs to the field of medical preparation for veterinary use; every 3g of the perfusion preparation contains 150-250 mg of amoxicillin, 50-125 mg of sulbactam, 5-15 mg of prednisolone and the rest of substrate; the substrate contains 0.1-12% of suspending aid, 0.1-2% of wetting agent, 0.01-1% of antioxidant, 0.05-1% of preservative and the rest of dispersion medium; the invention can be obtained via the following steps: firstly, dissolving or dispersing suspending aid, antioxidant, wetting agent and preservative into proper amount of dispersion medium, then slowly adding the rest of the dispersion medium into a colloid mill to obtain the substrate, adding medicine into the substrate, grinding via the alternating method of circularly grinding and non-circularly grinding. The preparation of the invention is simple in preparation technique, easy to realize industrialization, low in production cost, low in expenditure for treatment, low in toxicity, wide to use and high in efficiency; the invention can be clinically used for treating mammitis in lactation period caused by Gram positive bacterium and Gram negative bacterium.