194 results about "Bactericidal effect" patented technology

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide Mh-GC21 as well as a precursor and application thereof. The amino acid sequence of the antibacterial peptide Mh-GC21 provided by the invention is as shown in SEQ ID NO.1, and two cysteines are oxidized to form a pair of intermolecular disulfide bonds; the C tail end of the antibacterial peptide Mh-GC21 is subjected to amidation modification. The antibacterial peptide Mh-GC21 provided by the invention is from microhyla microcarpa, has broad-spectrum antibacterial activity, has relatively good antibacterial and bactericidal effects on standard strains of acinetobacter baumannii, escherichia coli and staphylococcus aureus and clinically sourced drug-resistant strains, and can be used for targeted destruction of cell membranes of bacteria, removal of biological membranes and inhibition of formation of the biological membranes. Moreover, the antibacterial peptide Mh-GC21 has the advantages of good stability, no hemolytic activity and cytotoxicity, and difficulty in inducing strains to generate drug resistance.

The invention relates to the technical field of sewage treatment, in particular to a preparation method of a degradable bactericide for an oil field. According to the invention, a hydroxypropyl guanidine gum-polycaprolactone-ethylenediamine fragment copolymer is taken as a carrier, a methylisothiazolinone quaternary ammonium salt (Q-MIT) bactericide is loaded, and the degradable bactericide for the oil field is obtained by cooperating with a dodecyl ethyl sulfide (TC-SE) bactericide and combining with sodium polyaspartate. A carrier forms a hydrogen bond with a polar group on the surface of a biological membrane through a hydroxypropyl guanidine gum main chain to realize targeted anchoring, a polycaprolactone branched chain encapsulates Q-MIT through a hydrophobic effect, primary amino groups of ethylenediamine are introduced to form a pH sensitive Schiff base bond with Q-MIT, and the primary amino groups and adipic dihydrazide form a thermal response hydrazide bond to construct a dual dynamic covalent bond, so that controlled release of Q-MIT is realized, and the biomembrane is prepared. And moreover, the components of the whole system are connected through degradable chemical bonds, so that the environmental protection property and sustainability of oil field operation are guaranteed.

The invention provides a tobacco leaf nitrogen efficient sesame cake fertilizer and a preparation method thereof, and relates to the field of fertilizers. The nitrogen efficient sesame cake fertilizer for tobacco leaves comprises a solid material and water, the solid material comprises the following raw materials in percentage by mass: 60%-70% of sesame cake, 6%-8% of cottonseed cake, 12%-26% of rice husk and 8%-10% of polyaspartic acid, wherein the total mass of the raw materials is 100%. The nitrogen efficient sesame cake fertilizer for the tobacco leaves can effectively improve the quality of the tobacco leaves, improve the growth vigor of the tobacco leaves and improve the hacking degree of flue-cured tobacco leaves to a certain extent, and the cottonseed cake is added due to the fact that gossypol with a bactericidal effect is contained in the cottonseed cake, and the occurrence of soil diseases can be relieved or inhibited.

The invention discloses an antibacterial peptide PA targeting multi-drug-resistant gram-negative bacteria and application of the antibacterial peptide PA, and the amino acid sequence of the antibacterial peptide PA is shown as SEQ ID NO.1. The antibacterial peptide PA provided by the invention has a remarkable antibacterial effect of the multi-drug-resistant gram-negative bacteria, is quick in bactericidal effect, does not have hemolysis and cytotoxicity, does not cause renal toxicity after treatment, and can be used for preparing the antibacterial peptide PA for treating the multi-drug-resistant gram-negative bacteria. The biological safety is good, and the compound can be used for treating bacterial infection and can also be applied to other scenes needing sterilization or bacterial growth inhibition.

The application belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide Mp-GC21, a precursor thereof and application. The amino acid sequence of the antibacterial peptide Mp-GC21 is shown as SEQ ID NO:1, wherein the C terminal is subjected to amidation modification, and two cysteines are connected through a disulfide bond; and the amino acid sequence of the precursor is shown as SEQ ID NO:3. The antibacterial peptide Mp-GC21 is separated from the flower frog, has broad-spectrum antibacterial activity, and has good bacteriostatic and bactericidal effects on standard strains and clinically derived drug-resistant strains of Acinetobacter baumannii, Escherichia coli and Staphylococcus aureus, and can target to destroy the cell membrane of bacteria, remove the biofilm and inhibit the formation of the biofilm. Moreover, the antibacterial peptide Mp-GC21 has good stability, does not have hemolytic activity, cytotoxicity and in-vivo toxicity, and is not easy to induce drug resistance of strains.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a method for improving the sensitivity of streptococcus mutans to a glycyrrhiza uralensis extract. According to the method, the glycyrrhiza uralensis extract and hydrogen ions jointly act on streptococcus mutans in an acid environment. Research finds that the inhibitory activity of part of the effective components of the glycyrrhiza uralensis extract on streptococcus mutans in an acid environment is remarkably enhanced. The glycyrrhiza uralensis extract and hydrogen ions have a synergistic effect, and the acidic environment can improve the sensitivity of the isostreptococcus to the glycyrrhiza uralensis extract and enhance the bactericidal effect.

This invention discloses the application of ferrous ions in the preparation of products for treating bacterial infections, belonging to the field of pharmaceutical technology. Antibacterial activity tests of ferrous ions against Staphylococcus aureus showed strong bactericidal effects, with a survival rate of less than 0.001% against Staphylococcus aureus and less than 0.01% against methicillin-resistant Staphylococcus aureus (MRSA). The use of ferrous ion-containing hydrogels in the treatment of keratitis and skin wound infections significantly reduced the risk of MRSA infection in the lungs and effectively prevented the proliferation and spread of microorganisms in the body, revealing that drugs containing ferrous compounds can be used to treat Staphylococcus aureus infections, including MRSA.

The invention relates to a veterinary hoof disease prevention and treatment composition as well as a preparation method and application thereof, and aims to solve the technical problems that existing commercial hoof bath lotion is limited in sterilization effect and relatively strong in local irritation, and a safe and efficient treatment medicament for treating cow hoof warts is lacked and the like. The invention provides a veterinary composition which comprises the following active ingredients: a glycyrrhizic acid compound, didecyl methyl ammonium bromide and polyhexamethylene guanidine salt, and the three components are combined, so that the composition has an antibacterial effect and an effect of promoting skin tissue repair at the same time. A series of tests prove that the traditional Chinese medicine composition can effectively inhibit animal pathogenic bacteria, prevent and / or treat animal limb and foot diseases and promote healing and repairing of ulcer injury. The medicament disclosed by the invention is suitable for preventing and treating limb and hoof diseases in a large-scale pasture, has the characteristics of high sterilization efficiency, good safety and effective treatment, and can improve the animal hoof health management level.

This invention relates to the field of oral care products technology, specifically to an artificial enzyme with antibacterial properties, its preparation method, and its application. This technical solution employs a chemical reduction method, where a palladium source reacts in an environment containing surfactants and reducing agents to obtain palladium nanozymes. The obtained palladium nanozymes exhibit uniform morphology, controllable particle size, good dispersibility in water, and good biocompatibility; they have no significant toxic side effects on human gingival fibroblasts, possess highly efficient catalase activity, and demonstrate strong antibacterial and bactericidal effects against oral pathogens, significantly reducing bacterial drug resistance; they can inhibit acid production by oral bacteria, preventing tooth decay; and they retain their original catalytic activity even after multiple cycles of use. Applying the palladium nanozymes of this solution to the manufacture of oral care products can solve the technical problems of unsatisfactory safety and easy development of drug resistance in existing oral care products using antibacterial and anti-caries methods, showing promising application prospects.

The invention relates to the technical field of bactericides for crops, in particular to a bacillus cereus-containing bactericidal composition and application thereof in prevention and control of camellia oleifera anthracnose. The active ingredients of the bactericidal composition comprise bacillus cereus WR-12 and diflumetofen sulfanilamide. According to the bactericidal composition, the synergistic bactericidal effect is enhanced by compounding a biological fungicide and a chemical fungicide, the development of drug resistance of pathogenic bacteria is delayed, the negative influence of chemical pesticides on the environment is reduced, green and efficient prevention and control of camellia oleifera anthracnose are achieved, and sustainable development of the camellia oleifera industry is promoted.

This invention discloses a method for preparing and applying a photothermal nanoreagent targeting bacteria. This invention utilizes Ti3C2T... x Using MXene as a matrix, a photothermal nanoreagent MXene@PDA@Peptide (MPP) was constructed by sequentially modifying the surface of Ti3C2Tx MXene with polydopamine and grafting it with the peptide CAEKA, which has the function of recognizing bacteria. Under the recognition of the peptide CAEKA, the photothermal Ti3C2Tx MXene was targeted. x MXene nanosheets are guided to the surface of bacterial cells, achieving effective sterilization at a relatively low overall temperature of around 50°C (or achieving more efficient sterilization at the same overall temperature level, exhibiting superior sterilization performance compared to pure Ti3C2Tx MXene). This makes them particularly suitable for the preparation of therapeutic drugs for subcutaneous abscesses caused by methicillin-resistant Staphylococcus aureus (MRSA) infection.

The present invention belongs to the technical field of pesticides, and specifically relates to a pyridine amide compound and the use thereof. The compound is as represented by formula I, wherein W1 is O or S; R1 is hydrogen, alkyl, alkenyl, alkynyl, etc.; R3, R4 and R5 are each independently hydrogen, halogen, alkyl, etc.; and X1, X2, X3, X 4, X5, Y1, Y2, X3 and Y4 are each independently hydrogen, halogen, alkyl, etc. The compound has a good bactericidal effect.

The invention relates to the technical field of biological medicine, in particular to a composition for blocking formation of phage treatment tolerance and application of the composition. The composition comprises a folium ilicis purpurea ethyl acetate extraction active component and a clostridium perfringens specific bacteriophage, the folium ilicis purpurea ethyl acetate extraction active component is prepared from the following components in percentage by mass: 8 to 15 percent of protocatechuic acid and 5 to 10 percent of ethyl gallate, and the mass ratio of the protocatechuic acid to the ethyl gallate is (1.5 to 1) to (2.5 to 1); the clostridium perfringens specific bacteriophage is a virulent bacteriophage capable of specifically cracking a clostridium perfringens NetB toxin positive strain. According to the invention, the shallow thought that the existing compounding technology only pursues synergistic sterilization is subverted, and a deep synergistic mode of bacteriophage splitting attack and folium ilicis purpurea component disintegration bacterial defense program is proposed and verified for the first time. And the formation of tolerance is fundamentally blocked, so that a more thorough and more lasting treatment effect is realized, and the comprehensive production performance of animals is accidentally and greatly improved on the basis of the treatment effect.

The present application relates to a kind of antibacterial polypeptide modified protein derivative and its preparation method.The protein derivative includes protein and antibacterial polypeptide, and the protein is connected between antibacterial polypeptide by triazolyl.The present application grafts antibacterial polypeptide with antibacterial activity to protein by click chemistry reaction, obtains antibacterial polypeptide modified protein derivative.The protein derivative has good biocompatibility, not only has good bactericidal effect, but also can avoid that bacteria produce drug resistance.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide Z34B3 and application thereof. The amino acid sequence of the antibacterial peptide Z34B3 provided by the invention is shown as SEQ ID NO: 1, the secondary structure is an alpha helix, the molecular weight is small, and the antibacterial peptide Z34B3 can change the cell membrane permeability of various gram-negative bacteria including pasteurella multocida, escherichia coli and acinetobacter baumannii, inhibit the generation of cell membranes and play antibacterial and bactericidal roles. Moreover, the antibacterial peptide Z34B3 provided by the invention has low hemolytic activity and no cytotoxicity, and can be applied to preparation of products with bacteriostatic and bactericidal effects and preparation of drugs for preventing and / or treating bacterial infectious diseases. Furthermore, the C tail end of the antibacterial peptide Z34B3 is subjected to amidation modification, and compared with non-modification treatment, the stability can be improved.

The application discloses a kind of composite drug-loaded nanoparticles based on calcium peroxide carrier, which is formed by the surface of hyaluronic acid modified calcium peroxide nanoparticles loaded with polylysine-dihydrophenophor e6 (Ce6) as carrier. pll The composite drug-loaded nanoparticles CaO2@HA / Ce6 are spherical particles with a particle size of about 100nm. pll In the application, Ce6 is converted into Ce6 by polylysine modification, and then combined with hyaluronic acid modified calcium peroxide nanoparticles. pll Ce6 is uniformly loaded on the surface of the composite drug-loaded nanoparticles CaO2@HA / Ce6. pll The application solves the problem that Ce6 cannot be attached to the surface of CaO2 nanoparticles. pll The prepared nanocomposite particles have uniform particle size and high specific surface area. pll After the combination of Ce6 and CaO2@HA, as a good bacterial microenvironment response material, the anoxic condition of bacterial site can be improved, and the photodynamic therapy can be enhanced. On the other hand, the biocompatibility and delivery efficiency of Ce6 are improved, so that significant bactericidal effect can be achieved at low dose.

This invention discloses the application of L-lysine in improving the sensitivity of Vibrio alginolyticus to clindamycin. This invention discovers that the combined use of L-lysine and clindamycin can significantly increase reactive oxygen species within Vibrio alginolyticus, thereby increasing the amount of clindamycin entering the bacteria and ultimately leading to bacterial death. Therefore, L-lysine can improve bacterial sensitivity to clindamycin, thus overcoming the problem of bacterial resistance. The small molecule metabolite provided by this invention, when used in combination with clindamycin, can significantly enhance the bactericidal effect of clindamycin, exhibiting better efficacy, higher safety, and greater operability compared to existing methods that use clindamycin alone as an antibacterial drug.

The invention belongs to the technical field of bioengineering, and particularly provides application of a JNK inhibitor in preparation of drugs for preventing and / or relieving and / or treating pulmonary tuberculosis aiming at the problem that whether the existing JNK inhibitor SP600125 can inhibit BNIP3-mediated mitochondrial autophagy and further exert the ROS bactericidal effect is unknown. And the JNK inhibitor is an SP600125 JNK inhibitor. The JNK inhibitor is used for inhibiting mitochondrial autophagy in the BCG infected macrophages. The JNK inhibitor reduces the up-regulation of BNIP3 and LC3B caused by BCG infection. It is found through the application that when the concentration of the JNK inhibitor reaches 15 umol / L, remarkable cytotoxicity begins to appear, and compared with a control group, the cell survival rate is remarkably decreased. The SP600125 can be used for inhibiting mitochondrial autophagy and down-regulating the expression of BNIP3 and LC3B. Inhibition of JNK expression can inhibit survival of Mtb in RAW264.7 macrophages, and a new thought and direction are provided for treatment of tuberculosis.

This invention relates to the field of biomedicine and hygiene disinfection products technology, and discloses a method for preparing a slightly acidic oxidizing potential hydrogel. The gel is made from slightly acidic oxidizing potential water and an inorganic gelling agent, lithium magnesium silicate. The preparation method includes: separately preparing strongly acidic oxidizing potential water and alkaline reducing potential water by electrolysis; mixing the two online in a preset ratio; adjusting the pH value to 5.0 to 6.5 through a neutralization reaction; subsequently adding lithium magnesium silicate; and then dispersing the mixture through high-speed shearing and sealing for static swelling to form a gel. This invention solves the problems of easy decomposition of available chlorine and gel liquefaction by utilizing an inorganic gel system, resulting in a gel with high stability and a long shelf life. This product solves the problem of easy loss of liquid disinfectants, prolongs the bactericidal effect time on wounds and oral ulcers, and is non-irritating and non-sensitizing to the skin, making it suitable for long-term disinfection of hands, skin, and mucous membranes.

This invention provides modified antimicrobial peptides, belonging to the field of antimicrobial peptide technology. The modified antimicrobial peptides include at least one of a first, second, third, fourth, fifth, and sixth antimicrobial peptide; the amino acid sequences of the first, second, third, fourth, fifth, and sixth antimicrobial peptides are shown sequentially as SEQ ID NO.1 to SEQ ID NO.6. Based on prevotellin-2, the modified antimicrobial peptides were obtained by reducing negatively charged amino acids, increasing positively charged amino acids, introducing hydrophobic amino acids, adjusting or deleting amino acid sequences, and optimizing the peptide structure. In vitro minimum inhibitory concentration (MIC) determination showed that, compared with the initial peptide, the modified antimicrobial peptides exhibited significantly enhanced antimicrobial activity against bacteria and fungi, and also showed significant bactericidal activity against clinically resistant bacteria.

This invention provides an oxidizing bactericide, its preparation method, and its application. The oxidizing bactericide comprises 30-60 parts of a modified chloramine compound, 5-15 parts of a modified chelating agent, 3-10 parts of a delay agent, 2-8 parts of a synergist, 1-5 parts of a corrosion inhibitor, and water to a total weight of 100 parts. The oxidizing bactericide of this invention exhibits stronger bactericidal effects, higher stability, and a longer shelf life. It also overcomes the drawback of traditional bactericides that easily corrode reusable membranes, significantly reducing the operation, maintenance, and consumable costs of water treatment systems, and possesses promising prospects for industrial application.

The invention discloses an emulsified cutting fluid using a plant extract as a bactericide. The emulsified cutting fluid is prepared from the following raw materials in parts by weight: 15-28 parts of base oil 1, 25-28 parts of base oil 2, 8-16 parts of an oil-soluble antirust agent, 10-19 parts of an emulsifier, 0.0-0.6 part of an antioxidant, 2-4 parts of an emulsion stabilizer and 3-5 parts of the plant extract bactericide, the base oil 1 is 150SN or transformer oil, the base oil 2 is naphthenic mineral oil, and the plant extract bactericide is a mixture of malus hupehensis extract, wolf thorn extract and melia azedarach extract. The special plant extract bactericide can effectively inhibit bacteria, fungi and anaerobic bacteria, has a broad-spectrum bactericidal effect, has good compatibility and emulsification stability, does not produce layering, turbidity and foam, does not destroy the uniformity and lubrication performance of the cutting fluid, can significantly reduce the skin irritation risk when workers contact the cutting fluid, and has a good application prospect. And the biodegradability is good, the biodegradability is easy to decompose by microorganisms in the natural environment, and the goal of'zero emission 'is favorably realized.

The invention discloses a botanical fungicide for metal working fluid, which is prepared from the following raw materials in parts by weight: malus hupehensis extract, wolf thorn extract and melia azedarach extract according to the mass ratio of 1: (2-4): (2-4). The bactericide is natural in raw materials, can effectively inhibit bacteria, fungi and anaerobic bacteria, has a broad-spectrum bactericidal effect, is good in stability, can effectively avoid the problem of spoilage of the metal working fluid, is good in compatibility with the metal working fluid, is not liable to generate a precipitation reaction with other additives in the working fluid, is not liable to generate drug resistance, and is safe and reliable. The skin irritation risk when workers make contact with the disinfectant can be remarkably reduced, the disinfectant has good biodegradability, is easily decomposed by microorganisms in the natural environment, does not leave lasting chemical residues like traditional bactericides, and is beneficial to achieving the zero emission target.

The livestock farm excrement treatment device comprises a shell, an inner cavity of the shell is a fermentation bin, an excrement collecting tank, a bacteriophage reaction chamber, an ultraviolet disinfection chamber, an exhaust purification module, a control module and a sensor group are arranged in the fermentation bin, the excrement collecting tank is arranged on the left side wall of the shell, and the bacteriophage reaction chamber is arranged on the left side wall of the shell. The shell is communicated with an inlet of the bacteriophage reaction chamber through a pipeline, an outlet of the bacteriophage reaction chamber is connected with the ultraviolet disinfection chamber through a pipeline, the ultraviolet disinfection chamber is communicated with the exhaust purification module arranged on the right side wall of the shell through a pipeline, the control module is arranged on the upper wall in the shell, and the sensor group is distributed on the ultraviolet disinfection chamber and the control module. According to the utility model, the bacteriophage is introduced into an excrement treatment system, so that pathogenic bacteria in excrement are specifically split, and the problems of poor animal excrement sterilization effect, limited drug-resistant bacterium inactivation effect, high possibility of generating harmful byproducts and the like in the prior art are solved.

The invention discloses a catechin silver nano antibacterial particle as well as a preparation method and application thereof, and belongs to the technical field of antibacterial materials. The silver catechin nano antibacterial particles disclosed by the invention are Ct-Ag NPs, and the silver catechin nano antibacterial particles are Ct-Ag NPs; the Ct-Ag NPs is of a spherical structure; the average particle size of the Ct-Ag NPs ranges from 142 nm to 162 nm; ag in the Ct-Ag NPs is uniformly dispersed in catechin to form a core-shell structure. The preparation method of the silver catechin nano antibacterial particles comprises the following steps: weighing catechin, and dissolving the catechin in a sodium hydroxide solution to obtain a catechin sodium salt solution; adding the silver nitrate solution into the ammonia water solution to obtain a silver ammonia solution; and under the conditions of stirring and ice bath, adding the catechin sodium salt solution into the silver ammonia solution, centrifuging, cleaning with trichloromethane, and drying to obtain the Ct-Ag NPs. The Ct-Ag NPs provided by the invention has a core-shell structure, and the quorum sensing inhibitor shell Ct can effectively reduce the generation of biological membranes, so that bacteria are more sensitive, and the bactericidal effect of continuously releasing Ag < + > is effectively improved.

This invention belongs to the field of biomedical functional materials technology, and relates to an antibacterial and healing-promoting photosensitizer, its preparation method, and its uses. The photosensitizer is a defective MIL-88B@MIL-88C composite material, wherein defective MIL-88B serves as the substrate, and MIL-88C grows on the surface and / or in the pores of the defective MIL-88B. This photosensitizer significantly improves the photogenerated carrier separation efficiency, reduces impedance, enhances photocurrent response, and can efficiently generate reactive oxygen species under light irradiation. Antibacterial experiments show that it has excellent bactericidal effects against Staphylococcus aureus, Escherichia coli, and methicillin-resistant Staphylococcus aureus, and is not prone to inducing drug resistance. Simultaneously, this photosensitizer has good biocompatibility, promotes cell migration, and has significant hemostatic function. Animal models show that it can effectively accelerate the healing of skin defects.

The invention belongs to the technical field of antibacterial materials, and particularly relates to a zwitterionic photosensitizer material, nanoparticles and a preparation method and application of the zwitterionic photosensitizer material and the nanoparticles. The structural formula of the zwitterionic photosensitizer material is shown in the specification, wherein x is any integer between 3 and 5; r1 and R2 groups are shown in the specification. The zwitterionic photosensitizer material based on sulfonation has excellent hydrophilicity and biological activity, can achieve an efficient sterilization effect at a low dosage, and has efficient curability on mouse living body wound infection, so that the zwitterionic photosensitizer material can be used for preparing a medicine for treating wound infection, and the problems that in the prior art, aggregation causes a fluorescence quenching effect, and the toxicity is poor are solved. And the problem of poor antibacterial treatment effect caused by reduction of ROS yield due to aggregation is solved.

The invention belongs to the technical field of prevention and control of drug-resistant bacteria, and particularly discloses a composite bactericidal composition and application thereof in preparation of a reagent for killing aeromonas hydrophila oxytetracycline drug-resistant strains. The minimum inhibitory concentration of the epsilon-polylysine to an aeromonas hydrophila oxytetracycline drug-resistant strain is as low as 3.125 g / mL. Meanwhile, the sterilization mechanism of the epsilon-polylysine on aeromonas hydrophila oxytetracycline drug-resistant strains is discussed through metabonomics research. On the basis, the application finds that various exogenous small molecule metabolites such as 2-phenylacetamide and the like are combined with epsilon-polylysine for use, so that the absorption of drug-resistant bacteria on epsilon-polylysine can be promoted, the sterilization effect of the epsilon-polylysine is effectively enhanced, and the problem of drug resistance of bacteria is solved. Therefore, when the epsilon-polylysine and the exogenous small molecule metabolite are jointly applied, compared with the existing single antibiotic therapy, the epsilon-polylysine has higher activity and better safety and operability in preparation of the reagent for resisting the aeromonas hydrophila oxytetracycline drug-resistant strain.

The invention provides a preparation method and application of alpinia oxyphylla leaf essential oil, and relates to the field of natural plant extracts. The alpinia oxyphylla leaf essential oil is applied to preparation of a bacteriostatic agent for inhibiting bacteria or / and fungi, and the bacteria comprise one or more of klebsiella pneumoniae, salmonella enterobacter and shigella flexneri. The fungi comprise one or more of candida tropicalis and malassezia furfur; the preparation method of the alpinia oxyphylla leaf essential oil comprises the following steps that alpinia oxyphylla leaves are taken, dried and smashed, the smashed alpinia oxyphylla leaves are placed in water to be soaked, the material-liquid mass ratio is 1: (15-17), and the soaking time is 0.8-1.2 h; after soaking, distillation is carried out, the distillation time is 4.5-5.5 h, and the distillation temperature is 170-200 DEG C; and after distillation is finished, collecting upper oily liquid to obtain the alpinia oxyphylla leaf essential oil. The prepared alpinia oxyphylla leaf essential oil has broad-spectrum antibacterial and bactericidal effects, and the antibacterial and bactericidal effects are good.