43 results about "Mastitis" patented technology

The invention discloses acinetobacter baumannii and application of the acinetobacter baumannii in construction of a mastitis animal model. The acinetobacter baumannii strain is acinetobacter baumannii AbST3475NMG202308, and the preservation number of the acinetobacter baumannii strain is CGMCC (China General Microbiological Culture Collection Center) No.34450 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms). Experiments prove that the acinetobacter baumannii has high pathogenicity and can be used for establishing a typical animal model caused by acinetobacter baumannii infection, the animal model can be specifically a mastitis animal model, and a certain technical support is provided for subsequent research and development of drugs or vaccines; the acinetobacter baumannii can also be used for screening antibacterial drugs and developing diagnostic reagents and vaccines. The method has an important application value.

The invention is applicable to the technical field of genetic engineering, and provides a vaccine for preventing and treating staphylococcus aureus mastitis and application of the vaccine. A recombinant bacillus subtilis construction method comprises the following steps: taking a DNA sequence as shown in SEQ ID NO.3 as a template, and performing PCR amplification to obtain a PCR product sequence; connecting the PCR product to a pHT43 vector, and converting the pHT43 vector into a competent cell DH5alpha, so as to obtain a recombinant plasmid pHT43-cCsa; the method comprises the following steps: transforming a recombinant plasmid pHT43-cCsa into a competent cell of bacillus subtilis WB800N, taking pHT43-F and pHT43-R as primers, and screening a positive transformant through a PCR (Polymerase Chain Reaction) technology; the recombinant bacillus subtilis for expressing the staphylococcus aureus Csa protein is constructed by utilizing a homologous recombination technology, so that pathological injury caused by staphylococcus aureus infection can be reduced, and staphylococcus aureus mastitis of dairy cattle can be prevented.

The present invention refers to the field of peptides, particularly with antimicrobial agent activity. Particularly, the present invention refers to peptides comprising the amino acid sequences as defined in the document, useful for treating and / or preventing an infection, preferably for treating and / or preventing animal mastitis, and / or as a preservative and / or as an immunomodulator. The present invention also refers to a set of peptides, to a composition, to uses of at least one peptide or the set of peptides, to a method for treating and / or preventing an infection and a kit.

According to the present invention, a technique which enables the production of an antimicrobial peptide Persulcatusin at reduced cost has been developed. A plant cell into which a polynucleotide sequence that encodes a gene for a protease to which a signal peptide localized in an intercellular organelle is added and a polynucleotide sequence that encodes a Persulcatusin fusion protein to which a signal peptide localized in an intracellular organelle different from the aforementioned intracellular organelle is added are introduced is produced, wherein, in the Persulcatusin fusion protein, a sequence that is cleavable with the protease is disposed between Persulcatusin and a protein that fuses to the Persulcatusin. Thus, Persulcatusin, which can serve as a therapeutic agent for bovine mastitis, can be produced at low cost.

The system comprises a laminated 5 diagnostic strip including a backing layer (105), an absorbent pad layer (106) configured for controlled capillary transport of milk, and a reagent deposit layer (107) defining multiple spatially separated dry reagent zones. The reagent zones include a pH indicator zone (101), a leukocyte esterase detection zone (102), a hemoglobin detection zone (103), and a nitrite detection zone (104), each adapted 10 for operation in the milk matrix. A calibrated sample application point (108) receives a predefined volume of untreated milk and fluidically communicates the sample to the reagent zones via the absorbent pad layer. Colorimetric reactions develop in a result window area (109), enabling simultaneous visual interpretation without analytical instruments, typically within three minutes. The invention further 15 includes a moisture-controlled storage container comprising desiccant elements (204, 205), a reference color chart (208), and a calibrated pipette (209), ensuring reagent stability and standardized use.

The application discloses a kind of treatment granulomatous lobular mastitis plaster and preparation method thereof, it is related to traditional Chinese medicine technical field, and its technical solution points are: rhubarb, golden poplar, radix scutellariae, sanguisorba officinalis, gardenia, turmeric, purple grass, water decoct 2 times, first time 9-11 times water amount of medicinal materials is added, decoct 1-3 hours, second time 6-9 times water amount of medicinal materials is added, decoct 1-2 hours, two water decocting liquids are combined, after filtration, concentrate and obtain ointment, carry out coating, cut according to specification and place to make it solidification and shape.The application can realize the treatment granulomatous lobular mastitis, can reduce mastitis inflammation index score and inflammatory cell infiltration degree, by reducing the expression of IL6, CD86, TYROBP, ITGAX and regulating related path, play the role of treatment granulomatous lobular mastitis.

The present application relates to the field of medical informatization and remote diagnosis and treatment technology, and discloses a curative effect evaluation and remote follow-up platform for mammitis ultrasonic interventional treatment. The platform integrates the imageological changes, physiological parameter trajectories, operation records and follow-up feedback of patients through a data processing module. A window recognition module dynamically divides curative effect observation windows and conventional observation windows according to treatment and feedback time points. A patient clustering module clusters patients based on the timing mode of physiology and images within the curative effect window, and identifies different response modes. A feature extraction module extracts typical operation features and follow-up response baselines of each patient community accordingly. A strategy generation module calculates the multidimensional matching degree by matching new patient data with the feature baselines of each community, and generates individualized follow-up strategies, thereby realizing adaptive and accurate definition of the curative effect evaluation window and individualized and directional optimization of follow-up management.

The invention discloses an inflammation regulation sequence of dairy cow mastitis and application thereof, and belongs to the technical field of biology, the inflammation regulation sequence is a regulation sequence responding to a gram-negative bacterium inflammation signal, the regulation sequence is a promoter sequence of a CXCL2 gene and is shown as SEQ ID NO: 1, and the regulation sequence is a promoter sequence of a CXCL2 gene and is shown as SEQ ID NO: 2. A regulatory sequence responding to a gram-positive bacterium inflammation signal, which is a promoter partial sequence of the NLRP3 gene and is as shown in SEQ ID NO: 2; the regulatory sequence responding to a gram-positive bacterium inflammation signal is a promoter reverse complementary sequence of the TLR2 gene and is shown as SEQ ID NO: 3; the endogenous inflammation regulation sequence provided by the invention is composed of genome elements of the dairy cow, and no exogenous coding gene is introduced, so that potential risks caused by exogenous proteins are avoided.

The invention relates to a preparation method and application of a lactating cow premix for improving a chronic stress state, and the lactating cow premix comprises the following components in percentage by mass: 25-40% of a carrier, 0.01-0.05% of an antioxidant, 2-8% of vitamins, 40-60% of minerals, 0.5-2% of probiotics, 1-5% of saccharomyces cerevisiae, 5-10% of lysine, 0.01-0.05% of chromium methionine and 2-5% of curcumin. The preparation method comprises the following steps: weighing the components in proportion, adding the components into a mixer, mixing for 20-40 minutes, packaging, and inspecting. The lactating dairy cow premix for improving the chronic stress state can effectively relieve the chronic stress condition of dairy cows, reduce the probability that the dairy cows suffer from mastitis and digestive tract diseases in the stress period, improve the resistance and immune function of the lactating dairy cows, increase the milk yield and improve the milk quality, and is balanced in formula nutrition, simple in preparation method and low in cost. The feed additive can be directly added into feed and has a wide application prospect.

The mastitis tissue fluid screening centrifugal machine comprises a machine box, a driving motor installed at the bottom of the machine box and a rotor fixedly connected to an output shaft of the driving motor, a plurality of installation openings are formed in the rotor in the circumferential direction of the rotor, clamping parts are arranged on the two sides of each installation opening, test tube bases with different tube diameters are arranged in the installation openings, and the clamping parts are arranged on the clamping parts. The clamping parts are provided with clamping assemblies for fixing the test tube seat, each clamping assembly comprises an abutting rod and a reset spring, the clamping parts are provided with through grooves allowing the abutting rods to movably stretch out to abut against the test tube seat, and the positions, corresponding to the abutting rods, of the test tube seat are provided with positioning grooves allowing the abutting rods to stretch into. A flange is arranged at the end part, far away from the test tube seat, of the abutting rod, and the abutting rod is in anti-rotation fit with the positioning groove and the through groove. According to the technical scheme, the test tube bases with different tube diameters are installed on the rotor so that the adaptability of the centrifugal machine can be improved, the clamping assembly can rapidly disassemble the single test tube base, and the whole rotor does not need to be disassembled.

Milk quality can be compromised by physiological conditions affecting dairy mammals, notably mastitis. Mastitis is an inflammatory disease of the mammary gland that may pose significant health and economic challenges. To address this, a system is proposed for monitoring at least one mammal to predict the onset of a clinical condition, such as, for example, mastitis. The system may be configured to sense one or more characteristics descriptive of mammal-related information, receive sensor data corresponding to those characteristics, and process the data to forecast the onset of mastitis within an upcoming time interval. Early detection enables timely intervention, promoting animal welfare and preserving milk quality.

A system and method for in-situ quantitative assessment of Bovine Mastitis (BM) in collected milk samples using optical imaging is disclosed. A bio-functionalized assay tray contains multiple wells, each having a gel with a specially prepared mixture of hemoglobin (Hb) and metal ions. At least one of the wells is filled with a reference milk sample which is characteristic of milk with no BM disease. Other wells are filled with collected milk samples. After waiting an interaction time, luminol and hydrogen peroxide solutions are added to the wells, causing chemiluminescent (CL) light emission. The intensity of CL light emitted by wells containing haptoglobin (Hp) is inhibited due to Hb-Hp binding. After waiting a CL light stabilization time, the tray is placed in a dark box, and a camera and image processor are used to analyze the CL light intensities. A calibration curve yields the Hp concentration in each well.

The present invention relates to a mutant strain of Escherichia coli or Staphylococcus aureus derived from bovine mastitis and a vaccine composition containing same. The vaccine composition according to the present invention may be usefully employed to reduce economic losses caused by bovine mastitis and to improve productivity and sustainability across the dairy industry.

The present application relates to the field of microbial technology, in particular to bacillus subtilis BS-1 and its application in water buffalo silage feed, the strain BS-1 of the present application has good bacteriostatic activity on escherichia coli, salmonella and streptococcus uberis, after mixing and fermenting according to a specific proportion with orange peel and cassava residue, the bacteriostatic spectrum of the obtained silage feed is significantly enhanced, which can simultaneously inhibit escherichia coli, campylobacter jejuni, salmonella, streptococcus uberis, staphylococcus aureus and streptococcus agalactiae that cause diarrhea and mastitis. In the feed, after mixing 60%-75% of orange peel and 25%-40% of cassava residue, the crude protein content of the fermentation product is significantly improved, and the fiber content is reduced. Feeding test shows that the feed can significantly improve the milk yield and milk quality of lactating water buffalo, and effectively alleviate diarrhea. The present application provides a safe, efficient and nutritious silage feed with disease prevention function.

The invention discloses a gel plaster for treating mastitis and a preparation method thereof, and relates to a traditional Chinese medicine composition technology, the gel plaster comprises a back lining layer, a drug-loaded gel layer and a cover lining layer, the drug-loaded gel layer comprises a gel matrix and drug components; the medicine components comprise chamomile, medicago falcata, dandelion, hollyhock seed, lithospermum erythrorhizon, commiphora mukukui, barley and lagofrog, and the mass ratio of chamomile to medicago falcata to dandelion to hollyhock seed to lithospermum erythrorhizon to commiphora mukukui to barley to lagofrog is 6: 6: 6: 6: 3: 3: 2: 6; the gel matrix mainly comprises a tackifier, a skeleton agent, glycerol, dihydroxyaluminum aminoacetate, EDTA (Ethylene Diamine Tetraacetic Acid) and a filling agent; the mass ratio of the tackifier to the skeleton agent to the glycerol to the dihydroxyaluminium aminoacetate to the EDTA to the filling agent to the medicinal components is 0.05 to 0.14 to 3.6 to 0.1 to 0.06 to 0.25 to 0.94. The gel plaster disclosed by the invention has a good treatment effect on mastitis, the use experience of a patient is good, and the gel plaster is convenient to carry and store.

The present application belongs to the field of alloy and medical devices, and relates to a multifunctional silver alloy probe and application thereof. The present application solves the core problems of traditional postpartum milk stasis and mastitis diagnosis, such as inconvenience, single function and insufficient biocompatibility, through the systematic innovation of silver-based alloy material innovation, interface integration technology of temperature / pressure dual sensor and instrument structure and precise preparation process, and provides an integrated medical device solution with functions of rapid detection, antibacterial protection and auxiliary dredging for the medical care field of lactating women, which has significant technical progress and clinical application value.

The invention relates to the technical field of veterinary drugs, in particular to a composition for preventing and / or treating donkey mastitis and application thereof. The invention provides a composition for preventing and / or treating donkey mastitis. The composition comprises the following components: nisin, lysozyme, a honeysuckle extract, a dandelion extract, a lithospermum extract, an astragalus extract and a frankincense extract. The composition can effectively prevent and / or treat donkey mastitis, the active ingredients of the composition are natural or biogenic substances, the antibacterial mechanism of the composition is unique, bacteria are not easily induced to generate drug resistance, and the composition is safe and free of residues, meets the urgent needs of green, healthy and sustainable development of animal husbandry, and has remarkable clinical application value and wide market prospects.

The present disclosure provides for compositions and methods for antimicrobial materials for automatic milking systems and dairy applications. In one aspect, the disclosure relates to composite compositions including an antimicrobial agent and a polymer. A non-limiting example of the disclosed antimicrobial agent is one that includes ZnO, and a non-limiting example of the disclosed polymer is one that includes a silicone polymer. The present disclosure also pertains to methods of making the disclosed composite composition, which in some aspects provides for even distribution of ZnO as disclosed herein. The present disclosure also pertains to methods of abrogating or eliminating mastitis in a dairy herd including using a dairy liner in milking operation including a disclosed composite composition. The present disclosure further pertains to products including the disclosed composite composition. In some aspects, the disclosed products include a dairy liner used in a milking operation.

The present invention relates to a combination of lysobacin and an aminoglycoside antibiotic for use in the treatment of diseases caused by Gram-positive and Gram-negative bacteria in non-human animals. The present invention is demonstrated to be particularly suitable for use involving the treatment of mastitis in non-human animals. Especially, bovine mastitis can be well solved by the traditional Chinese medicine.

This invention introduces the use of high dissolved oxygen levels exceeding 185%, delivered through fluids, aqueous solutions, gels, hydrogels, creams, aerosol sprays or micro- encapsulation, for treatment of wounds, burns, ischemia, neuropathy, skin-related conditions, skin grafts, and bovine mastitis. Unlike traditional Hyperbaric Oxygen Therapy (HBOT), this method provides a targeted, noninvasive approach, ensuring optimal oxygen availability at the cellular level. Unlike traditional HBOT the patient does not have to be referred for treatment and can self-treat at home. The composition enhances therapeutic outcomes by promoting cell proliferation, reepithelialization, collagen synthesis, and angiogenesis while reducing infection risks and inflammation. The vehicle stabilizes the high dissolved oxygen and facilitates its effective application. This invention addresses the limitations of existing oxygen delivery mechanisms, offering a versatile and efficient solution for tissue oxygenation and accelerated healing in both human and veterinary medicine.

The invention discloses application of alpha-hederin in preparation of a medicine for preventing or treating staphylococcus aureus infectious diseases, and belongs to the technical field of medicines. The staphylococcus aureus infectious disease is one of skin or soft tissue infection, pneumonia, osteoarthritis, mastitis, enteritis, endocarditis, urinary tract infection and enteritis. The medicine does not have an in-vitro direct sterilization function on sensitive and methicillin-resistant staphylococcus aureus, but can remarkably promote infected cells to remove thalli under a cell infection condition, so that the medicine plays a role in resisting sensitive and methicillin-resistant staphylococcus aureus infection by improving the anti-infection immune level of the cells.

The application discloses a kind of external use traditional Chinese medicine preparation for treating cold blood stasis type granulomatous lobular mastitis and preparation method, and external use traditional Chinese medicine preparation is made of the raw materials including the following medicinal materials: prepared rhizome of rehmannia, antler slice, cassia, gunpowder ginger, chuanxiong, safflower, golden cypress, radix angelicae pubescentis, spironolactone, white and so on.The external preparation formula of the application is warm and blood tonic, blood-activating and skin-regenerating, has better clinical effect of treating cold blood stasis type granulomatous lobular mastitis, can effectively reduce the size of mass in clinical test, improve patient life treatment score, reduce the pain of patient, and has greater popularization and application value.

The present application relates to the use of substituted indazoles for treatment and / or prophylaxis of allergic and / or inflammatory diseases in animals, especially for treatment and / or prophylaxis of atopic dermatitis, Flea Allergy Dermatitis, inflammatory bowel disease, osteoarthritis and inflammatory pain, non-infectious recurrent airway disease, insect hypersensitivity, asthma, respiratory disease, mastitis and endometritis in animals.

This invention relates to methods of treatment and prevention of mastitis in animals by orthosomycin antimicrobial compounds.

The invention discloses a hand holographic reflectometer, and relates to the technical field of hand health care instruments. The massager comprises a shell structure composed of a lower shell plate, an upper shell and an outer shell plate, the top of the lower shell plate is provided with a palm clamping position, and the bottom of the lower shell plate is provided with an air pump, a lower air valve and a steering engine (motor); the motor drives the massage block with the rack through the cone to achieve up-down massage motion. An upper air bag is arranged between the upper shell and the outer shell plate, and a pressure movement push plate is attached to the lower portion of the middle upper air bag. By means of the mode of combining pneumatic transmission and mechanical transmission, accurate massage of muscle areas, corresponding to human viscera and organs, of palms is achieved, and by combining the palm muscle reflex theory, sore throat, stomachache, lumbago, upper and lower toothache, stiff neck, shoulder discomfort, pain caused by mastitis, dysmenorrhea and pain caused by haemorrhoids can be effectively relieved; the device has the advantages of being noninvasive, adjustable in strength, quick in effect taking and the like.

The invention belongs to the technical field of traditional Chinese veterinary medicines, and relates to a traditional Chinese medicine composition for treating stasis-heat intermingled cattle and sheep mastitis and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 130 to 170 parts of herba violae, 130 to 170 parts of honeysuckle stem, 90 to 110 parts of cowherb seed, 50 to 70 parts of radix bupleuri herb, 75 to 105 parts of radix paeoniae rubra, 50 to 70 parts of fructus crataegi, 40 to 60 parts of spina gleditsiae and 25 to 35 parts of liquorice root. The traditional Chinese medicine composition provided by the invention is based on the theory of stagnant heat competing, is precise and appropriate in compatibility, and has the advantages of remarkable curative effect, safety, economy and the like, so that the recovery of the milk production performance of dairy cows or milch goats can be better ensured.

The application discloses a pharmaceutical composition containing miRNA mimics, and a preparation method and application thereof. The application develops a pharmaceutical composition capable of being used for preventing and treating bovine mastitis, and the pharmaceutical composition contains a first miRNA mimic and a second miRNA mimic. In-vitro experimental results show that the pharmaceutical composition has the function of inhibiting cell inflammatory response. Further in-vivo experimental results show that the combination of the miRNA mimics can obviously reduce inflammatory infiltration of mammary gland tissues, interstitial edema and alveolar damage degree, thereby effectively treating bovine mastitis. Compared with a traditional treatment method, the application has the advantages of high targeting, high safety, clear action mechanism and the like, and has a wide application prospect in the field of veterinary clinical prevention and treatment.

A sustained release solid oral bolus that, once ingested, is capable of providing sustained release of a therapeutically effective amount of a non-steroidal anti-inflammatory drug (NSAID), and its use for treating fever, pain and / or inflammation, preferably caused by periparturient disorders, such as mastitis, lameness, endometritis, metritis, or subfertility.

The utility model discloses a postpartum bra, which belongs to the technical field of medical auxiliary articles, can be used for accurately applying medicine to a puerpera suffering from mastitis, and has the characteristics of convenience in use and good treatment effect. Comprising a pair of symmetrically-arranged cups and a breast supporting belt, the cups are fixedly connected with the breast supporting belt, each cup is sequentially provided with a dressing layer piece and a first isolation layer piece from inside to outside, and nipple through holes are formed in the middles of the dressing layer pieces and the middles of the first isolation layer pieces. The dressing layer piece is sequentially provided with a first dressing area and a second dressing area in the direction from the nipple through hole to the outer edge, the first dressing area and the second dressing area are each internally provided with four dressing holes, and the lower edge of each dressing hole is provided with a sponge strip. The nipple through hole in the dressing layer piece and the outer edge of the dressing hole are both connected with the first isolation layer piece in a sealed mode.