76 results about "Mastitis" patented technology

The invention discloses acinetobacter baumannii and application of the acinetobacter baumannii in construction of a mastitis animal model. The acinetobacter baumannii strain is acinetobacter baumannii AbST3475NMG202308, and the preservation number of the acinetobacter baumannii strain is CGMCC (China General Microbiological Culture Collection Center) No.34450 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms). Experiments prove that the acinetobacter baumannii has high pathogenicity and can be used for establishing a typical animal model caused by acinetobacter baumannii infection, the animal model can be specifically a mastitis animal model, and a certain technical support is provided for subsequent research and development of drugs or vaccines; the acinetobacter baumannii can also be used for screening antibacterial drugs and developing diagnostic reagents and vaccines. The method has an important application value.

The invention discloses lactobacillus paracasei CFCFC018, a microbial agent and application of the lactobacillus paracasei CFCFC018, and belongs to the technical field of microorganisms. The lactobacillus paracasei CFCFC018 is separated from intestinal contents of rural cats in half-farming and half-pastoral areas in Inner Mongolia, does not need genetic engineering modification, can highly express amino acids and neurotransmitters, can remarkably relieve mammary gland alveolar structure damage in mammary gland tissues of rats suffering from mastitis induced by staphylococcus aureus, and has the advantages of being high in safety and good in stability. The thickening of a mammary gland interacellular matrix layer is avoided, the infiltration number of neutrophils is reduced, the damage degree of mammary gland tissues is reduced, and a certain protection effect on the mammary gland tissues is achieved. The research also shows that the strain can reduce the abundance of harmful bacteria in excrement, improve intestinal inflammation and relieve edema of intestinal submucosa, which provides a new thought for preventing and treating mastitis by regulating intestinal microbiota.

The invention specifically discloses three SNP (Single Nucleotide Polymorphism) molecular markers obviously related to Chinese Holstein cattle somatic cell scoring (SCS) and application thereof. The SNP is located in the following aspects: 1) in a No.8 chromosome 15818198bp (LINGO2 gene, Ggt; t), genotype effect GGlt; gTlt; GTlt; tT; 2), in the 17th chromosome 16535314bp (Ggt in the ZNF330 gene; a), effect GGlt; aA1t; AA1t; gA; 3) in the 69672126bp (in the PACS2 gene, Ggt; a), effect GGlt; gAlt; aA. SCS is an index of mastitis, and high SCS corresponds to high morbidity rate of mastitis. By detecting the genotypes of the sites, individuals with low SCS (namely, low mastitis morbidity) can be accurately bred in the early stage, the disease-resistant breeding efficiency and accuracy are remarkably improved, the reduction of the mastitis morbidity of groups is accelerated, and the production performance and economic benefits are improved.

The invention belongs to the field of detection, and particularly relates to a kit for detecting and identifying staphylococcus capitis and a detection method. The kit for detecting and identifying the staphylococcus capitis comprises Taq DNA (deoxyribonucleic acid) polymerase, a 10 * Taq buffer solution, a dNTP (deoxyribonucleoside triphosphate) mixed solution, an upstream primer, a downstream primer and a DNA Marker III. During detection, DNA of a sample to be detected is taken as a template, electrophoresis is performed after PCR amplification, if a 863bp band appears, the sample is positive, rapid and specific detection can be performed, and clinical (such as non-lactation period mastitis) requirements are met.

The invention is applicable to the technical field of genetic engineering, and provides a vaccine for preventing and treating staphylococcus aureus mastitis and application of the vaccine. A recombinant bacillus subtilis construction method comprises the following steps: taking a DNA sequence as shown in SEQ ID NO.3 as a template, and performing PCR amplification to obtain a PCR product sequence; connecting the PCR product to a pHT43 vector, and converting the pHT43 vector into a competent cell DH5alpha, so as to obtain a recombinant plasmid pHT43-cCsa; the method comprises the following steps: transforming a recombinant plasmid pHT43-cCsa into a competent cell of bacillus subtilis WB800N, taking pHT43-F and pHT43-R as primers, and screening a positive transformant through a PCR (Polymerase Chain Reaction) technology; the recombinant bacillus subtilis for expressing the staphylococcus aureus Csa protein is constructed by utilizing a homologous recombination technology, so that pathological injury caused by staphylococcus aureus infection can be reduced, and staphylococcus aureus mastitis of dairy cattle can be prevented.

The invention discloses a primer group and a kit for high-flux targeted detection of pathogens and drug-resistant genes of camel mastitis and application of the primer group and the kit. The primer group consists of 185 pairs of primers with nucleotide sequences as shown in SEQ ID NO: 1-370. According to the primer group, the kit and the detection method disclosed by the invention, specific amplification is carried out on key areas of 20 pathogen target genes and 77 drug-resistant genes of camel mastitis; the kit has the following remarkable advantages: 1) high precision and specificity: sequencing is performed for a specific region, so that the detection accuracy is remarkably improved, low-abundance pathogenic bacterium nucleic acid can be effectively detected, and early diagnosis is assisted; 2) high efficiency and economy: parallel detection of various pathogenic bacteria and drug-resistant genes can be realized through a single experiment, and the detection flux is greatly improved; compared with whole genome sequencing, the cost of targeted sequencing is lower; 3) the method is simple, convenient and universal, the data analysis complexity is remarkably reduced, the method is suitable for various clinical samples such as blood, tissues and secretions, and a unified and efficient detection scheme is provided for different sample types.

The present invention refers to the field of peptides, particularly with antimicrobial agent activity. Particularly, the present invention refers to peptides comprising the amino acid sequences as defined in the document, useful for treating and / or preventing an infection, preferably for treating and / or preventing animal mastitis, and / or as a preservative and / or as an immunomodulator. The present invention also refers to a set of peptides, to a composition, to uses of at least one peptide or the set of peptides, to a method for treating and / or preventing an infection and a kit.

According to the present invention, a technique which enables the production of an antimicrobial peptide Persulcatusin at reduced cost has been developed. A plant cell into which a polynucleotide sequence that encodes a gene for a protease to which a signal peptide localized in an intercellular organelle is added and a polynucleotide sequence that encodes a Persulcatusin fusion protein to which a signal peptide localized in an intracellular organelle different from the aforementioned intracellular organelle is added are introduced is produced, wherein, in the Persulcatusin fusion protein, a sequence that is cleavable with the protease is disposed between Persulcatusin and a protein that fuses to the Persulcatusin. Thus, Persulcatusin, which can serve as a therapeutic agent for bovine mastitis, can be produced at low cost.

The system comprises a laminated 5 diagnostic strip including a backing layer (105), an absorbent pad layer (106) configured for controlled capillary transport of milk, and a reagent deposit layer (107) defining multiple spatially separated dry reagent zones. The reagent zones include a pH indicator zone (101), a leukocyte esterase detection zone (102), a hemoglobin detection zone (103), and a nitrite detection zone (104), each adapted 10 for operation in the milk matrix. A calibrated sample application point (108) receives a predefined volume of untreated milk and fluidically communicates the sample to the reagent zones via the absorbent pad layer. Colorimetric reactions develop in a result window area (109), enabling simultaneous visual interpretation without analytical instruments, typically within three minutes. The invention further 15 includes a moisture-controlled storage container comprising desiccant elements (204, 205), a reference color chart (208), and a calibrated pipette (209), ensuring reagent stability and standardized use.

The application discloses a kind of treatment granulomatous lobular mastitis plaster and preparation method thereof, it is related to traditional Chinese medicine technical field, and its technical solution points are: rhubarb, golden poplar, radix scutellariae, sanguisorba officinalis, gardenia, turmeric, purple grass, water decoct 2 times, first time 9-11 times water amount of medicinal materials is added, decoct 1-3 hours, second time 6-9 times water amount of medicinal materials is added, decoct 1-2 hours, two water decocting liquids are combined, after filtration, concentrate and obtain ointment, carry out coating, cut according to specification and place to make it solidification and shape.The application can realize the treatment granulomatous lobular mastitis, can reduce mastitis inflammation index score and inflammatory cell infiltration degree, by reducing the expression of IL6, CD86, TYROBP, ITGAX and regulating related path, play the role of treatment granulomatous lobular mastitis.

The present application relates to the field of medical informatization and remote diagnosis and treatment technology, and discloses a curative effect evaluation and remote follow-up platform for mammitis ultrasonic interventional treatment. The platform integrates the imageological changes, physiological parameter trajectories, operation records and follow-up feedback of patients through a data processing module. A window recognition module dynamically divides curative effect observation windows and conventional observation windows according to treatment and feedback time points. A patient clustering module clusters patients based on the timing mode of physiology and images within the curative effect window, and identifies different response modes. A feature extraction module extracts typical operation features and follow-up response baselines of each patient community accordingly. A strategy generation module calculates the multidimensional matching degree by matching new patient data with the feature baselines of each community, and generates individualized follow-up strategies, thereby realizing adaptive and accurate definition of the curative effect evaluation window and individualized and directional optimization of follow-up management.

1. The name of the design product: medical version of massage and milk discharge protective clothing. 2. The use of the design product: medical staff wear it when performing massage and milk discharge operation for lactation mastitis patients, effectively shielding the body and preventing contamination caused by milk spraying. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: front view. 5. The design product is a thin product, and the left view, right view, top view and bottom view are omitted.

The application discloses a milk collecting system and a collecting method, and relates to the field of milk collecting systems, and aims to realize the functions of measuring the conductivity of milk, monitoring whether a dairy cow is suffering from mastitis, and timely controlling the pollution of other milk by the milk produced by the dairy cow suffering from mastitis.

The present application relates to the use of a strain of breast milk-derived Limosilactobacillus fermentum MSJK0025 in the improvement of breast health (improvement in mastitis, and prevention and / or treatment of breast cancer) in the technical field of microorganisms. Specifically provided are a pharmaceutical composition containing breast milk-derived Limosilactobacillus fermentum MSJK0025 for improving breast health, and a probiotic microbial agent containing breast milk-derived Limosilactobacillus fermentum MSJK0025 for improving breast health. Provided in the present application is the new use of a strain of breast milk-derived Limosilactobacillus fermentum MSJK0025 in the improvement of breast health. Limosilactobacillus fermentum MSJK0025 can be used to effectively improve breast health.

The invention discloses an inflammation regulation sequence of dairy cow mastitis and application thereof, and belongs to the technical field of biology, the inflammation regulation sequence is a regulation sequence responding to a gram-negative bacterium inflammation signal, the regulation sequence is a promoter sequence of a CXCL2 gene and is shown as SEQ ID NO: 1, and the regulation sequence is a promoter sequence of a CXCL2 gene and is shown as SEQ ID NO: 2. A regulatory sequence responding to a gram-positive bacterium inflammation signal, which is a promoter partial sequence of the NLRP3 gene and is as shown in SEQ ID NO: 2; the regulatory sequence responding to a gram-positive bacterium inflammation signal is a promoter reverse complementary sequence of the TLR2 gene and is shown as SEQ ID NO: 3; the endogenous inflammation regulation sequence provided by the invention is composed of genome elements of the dairy cow, and no exogenous coding gene is introduced, so that potential risks caused by exogenous proteins are avoided.

The invention relates to a preparation method and application of a lactating cow premix for improving a chronic stress state, and the lactating cow premix comprises the following components in percentage by mass: 25-40% of a carrier, 0.01-0.05% of an antioxidant, 2-8% of vitamins, 40-60% of minerals, 0.5-2% of probiotics, 1-5% of saccharomyces cerevisiae, 5-10% of lysine, 0.01-0.05% of chromium methionine and 2-5% of curcumin. The preparation method comprises the following steps: weighing the components in proportion, adding the components into a mixer, mixing for 20-40 minutes, packaging, and inspecting. The lactating dairy cow premix for improving the chronic stress state can effectively relieve the chronic stress condition of dairy cows, reduce the probability that the dairy cows suffer from mastitis and digestive tract diseases in the stress period, improve the resistance and immune function of the lactating dairy cows, increase the milk yield and improve the milk quality, and is balanced in formula nutrition, simple in preparation method and low in cost. The feed additive can be directly added into feed and has a wide application prospect.

The mastitis tissue fluid screening centrifugal machine comprises a machine box, a driving motor installed at the bottom of the machine box and a rotor fixedly connected to an output shaft of the driving motor, a plurality of installation openings are formed in the rotor in the circumferential direction of the rotor, clamping parts are arranged on the two sides of each installation opening, test tube bases with different tube diameters are arranged in the installation openings, and the clamping parts are arranged on the clamping parts. The clamping parts are provided with clamping assemblies for fixing the test tube seat, each clamping assembly comprises an abutting rod and a reset spring, the clamping parts are provided with through grooves allowing the abutting rods to movably stretch out to abut against the test tube seat, and the positions, corresponding to the abutting rods, of the test tube seat are provided with positioning grooves allowing the abutting rods to stretch into. A flange is arranged at the end part, far away from the test tube seat, of the abutting rod, and the abutting rod is in anti-rotation fit with the positioning groove and the through groove. According to the technical scheme, the test tube bases with different tube diameters are installed on the rotor so that the adaptability of the centrifugal machine can be improved, the clamping assembly can rapidly disassemble the single test tube base, and the whole rotor does not need to be disassembled.

A design is an apparatus for protecting dairy cows against mastitis by enabling rapid closure of the ends of the teats, which remain open for at least two hours after milking, to prevent them from being exposed to bacteria.

The Chinese herbal medicine feed additive comprises a formula I and a formula II, the formula I comprises immature bitter orange, roasted rhizoma atractylodis macrocephalae, radix salviae miltiorrhizae, radix astragali, scorched medicated leaven, liquorice root, cowherb seed and akebiaquinata; the formula II comprises astragalus membranaceus, cistanche deserticola, herba epimedii, glossy privet fruit, angelica sinensis, codonopsis pilosula, roasted rhizoma atractylodis macrocephalae, poria cocos and scorched medicated leaven. The invention provides two Chinese herbal medicine feed additives for sows, on one hand, the Chinese herbal medicine feed additives can promote digestion and absorption, promote menstruation and promote lactation; on the other hand, postpartum recovery of sows can be promoted and estrus cycle is shortened; meanwhile, the occurrence of mastitis and other diseases is reduced.

Milk quality can be compromised by physiological conditions affecting dairy mammals, notably mastitis. Mastitis is an inflammatory disease of the mammary gland that may pose significant health and economic challenges. To address this, a system is proposed for monitoring at least one mammal to predict the onset of a clinical condition, such as, for example, mastitis. The system may be configured to sense one or more characteristics descriptive of mammal-related information, receive sensor data corresponding to those characteristics, and process the data to forecast the onset of mastitis within an upcoming time interval. Early detection enables timely intervention, promoting animal welfare and preserving milk quality.

A system and method for in-situ quantitative assessment of Bovine Mastitis (BM) in collected milk samples using optical imaging is disclosed. A bio-functionalized assay tray contains multiple wells, each having a gel with a specially prepared mixture of hemoglobin (Hb) and metal ions. At least one of the wells is filled with a reference milk sample which is characteristic of milk with no BM disease. Other wells are filled with collected milk samples. After waiting an interaction time, luminol and hydrogen peroxide solutions are added to the wells, causing chemiluminescent (CL) light emission. The intensity of CL light emitted by wells containing haptoglobin (Hp) is inhibited due to Hb-Hp binding. After waiting a CL light stabilization time, the tray is placed in a dark box, and a camera and image processor are used to analyze the CL light intensities. A calibration curve yields the Hp concentration in each well.

Embodiments relate to a composition comprising a bacteriophage or a mutant thereof and a storage solution, wherein the bacteriophage or the mutant thereof is capable of killing a Gram-positive bacterium comprising Staphylococcus aureus, wherein the composition is stable for at least about a year at 39.2-46.4° F. (4-8° C.).

The present invention relates to a mutant strain of Escherichia coli or Staphylococcus aureus derived from bovine mastitis and a vaccine composition containing same. The vaccine composition according to the present invention may be usefully employed to reduce economic losses caused by bovine mastitis and to improve productivity and sustainability across the dairy industry.

The present application relates to the field of microbial technology, in particular to bacillus subtilis BS-1 and its application in water buffalo silage feed, the strain BS-1 of the present application has good bacteriostatic activity on escherichia coli, salmonella and streptococcus uberis, after mixing and fermenting according to a specific proportion with orange peel and cassava residue, the bacteriostatic spectrum of the obtained silage feed is significantly enhanced, which can simultaneously inhibit escherichia coli, campylobacter jejuni, salmonella, streptococcus uberis, staphylococcus aureus and streptococcus agalactiae that cause diarrhea and mastitis. In the feed, after mixing 60%-75% of orange peel and 25%-40% of cassava residue, the crude protein content of the fermentation product is significantly improved, and the fiber content is reduced. Feeding test shows that the feed can significantly improve the milk yield and milk quality of lactating water buffalo, and effectively alleviate diarrhea. The present application provides a safe, efficient and nutritious silage feed with disease prevention function.

The invention discloses citrus reiculata Blanco fruit fiber probiotic feed as well as a preparation method and application thereof, and relates to the technical field of feed. The feed is mainly prepared from citrus reiculata blanco residues, hay, flaxseed meal, tea residues, corn flour and soybean meal through compound microorganism fermentation. The feed comprises the following fermentation raw materials in percentage by weight: 60-75% of citrus reiculata Blanco residues, 20-30% of hay, 5-15% of flaxseed meal, 8-10% of tea residues, 2-10% of corn flour and 5-12% of soybean meal. The compound microorganisms are aspergillus niger, saccharomyces cerevisiae, corynebacterium glutamicum and lactobacillus plantarum. By optimizing the fermentation raw materials, the fermentation inoculant and the fermentation method, not only are the contents of crude protein, crude fat and amino acid in the citrus reiculata blanco residues increased, but also the content of anti-inflammatory and antioxidant substances is increased, and when the citrus reiculata blanco residues are used for feeding dairy cows, sufficient nutrients easy to digest and absorb can be provided, the immunity of the dairy cows is improved, and the morbidity of mastitis is reduced; therefore, the milk yield of the dairy cow is improved.

The invention relates to the field of treatment of mastitis in cattle and sheep breeding in biological agriculture, in particular to a pure traditional Chinese medicine oral administration and external application dual-way medicine for cattle and sheep intestinal mastitis. The oral prescription comprises the following main medicines in parts by weight: 15-18 parts of dandelion, 17 parts of salvia miltiorrhiza, 12 parts of pomegranate peel extract and 15 parts of attapulgite; the oral composition adjuvant comprises 6-8 parts of asparagus, 5-7 parts of ligusticum wallichii, 5 parts of an echinacea extract or 5 parts of astragalus polysaccharide; the ratio of lactobacillus plantarum to lactobacillus acidophilus compound bacterium powder is 2: 1 (15 parts); and the external application formula comprises 48 parts of main medicines: 15 parts of selfheal, 15 parts of philippine violet herb, 8 parts of ricepaperplant pith, 8 parts of frankincense and 2 parts of baicalin. According to the device, the medicine external application function and the milking function are combined, the treatment efficiency is improved, the stress response to animals is reduced, and the whole treatment process is more humanized and efficient.

The invention discloses a gel plaster for treating mastitis and a preparation method thereof, and relates to a traditional Chinese medicine composition technology, the gel plaster comprises a back lining layer, a drug-loaded gel layer and a cover lining layer, the drug-loaded gel layer comprises a gel matrix and drug components; the medicine components comprise chamomile, medicago falcata, dandelion, hollyhock seed, lithospermum erythrorhizon, commiphora mukukui, barley and lagofrog, and the mass ratio of chamomile to medicago falcata to dandelion to hollyhock seed to lithospermum erythrorhizon to commiphora mukukui to barley to lagofrog is 6: 6: 6: 6: 3: 3: 2: 6; the gel matrix mainly comprises a tackifier, a skeleton agent, glycerol, dihydroxyaluminum aminoacetate, EDTA (Ethylene Diamine Tetraacetic Acid) and a filling agent; the mass ratio of the tackifier to the skeleton agent to the glycerol to the dihydroxyaluminium aminoacetate to the EDTA to the filling agent to the medicinal components is 0.05 to 0.14 to 3.6 to 0.1 to 0.06 to 0.25 to 0.94. The gel plaster disclosed by the invention has a good treatment effect on mastitis, the use experience of a patient is good, and the gel plaster is convenient to carry and store.

A teat sealant composition free of bismuth salts, a method for preparing the same, and a method for preventing new intra-breast infections or mastitis.

The present application belongs to the field of alloy and medical devices, and relates to a multifunctional silver alloy probe and application thereof. The present application solves the core problems of traditional postpartum milk stasis and mastitis diagnosis, such as inconvenience, single function and insufficient biocompatibility, through the systematic innovation of silver-based alloy material innovation, interface integration technology of temperature / pressure dual sensor and instrument structure and precise preparation process, and provides an integrated medical device solution with functions of rapid detection, antibacterial protection and auxiliary dredging for the medical care field of lactating women, which has significant technical progress and clinical application value.