176 results about "Nitrite" patented technology

The present invention belongs to the technical fields of nanomaterials, analytical detection, and food safety, and specifically relates to a single-atom POD-mimicking enzyme nanomaterial, a preparation method thereof, and a food safety detection application. The single-atom POD-mimicking enzyme nanomaterial comprises a noble metal Os and a defective metal oxide, wherein the defective metal oxide is an oxide of metal Cu, and the Os is anchored on the defective metal oxide in the form of single atoms. The present invention also relates to the use of the single-atom POD-mimicking enzyme nanomaterial in the preparation of a detection reagent having POD activity and in the colorimetric detection of hydrogen peroxide or nitrite.

The invention discloses a sodium iodide probe assisted gold nanoparticle-polyarginine-polylysine electrochemical sensor and an application thereof. The composite electrode provided by the invention comprises an electrode substrate and a nano composite film modified on the surface of the electrode substrate, the nano composite membrane comprises gold nanoparticles, polyarginine and polylysine. The invention further provides an electrochemical sensor which is constructed by combining the sodium iodide probe and the composite electrode and is used for detecting nitrite. The electrochemical sensor has the advantages of simplicity, rapidness, low detection limit, wide response range, good reproducibility, high selectivity and the like. The electrochemical sensor can be used for measuring the content of nitrite in beef and tap water samples, the recovery rate is satisfactory, and the electrochemical sensor has potential application prospects in the aspects of food safety and environmental protection.

The invention relates to the technical field of biological denitrification, in particular to a nitrogen-doped carbon dot-based microbial electronic control denitrification system and application thereof. The system comprises electron donor microorganisms, electron acceptor microorganisms and nitrogen-doped carbon dots, the nitrogen-doped carbon dots serve as electron mediators outside cells, an efficient extracellular electron transfer network is constructed, and interspecific electron transfer resistance is reduced; meanwhile, the nitrogen-doped carbon dots can regulate and control intracellular electron transport chains and the energy metabolism process, the improvement of the reducing power and the energy level is promoted, and the metabolism mode optimization is realized. By cooperatively regulating and controlling generation, transmission and utilization of electrons under an extreme electron donor limited oligotrophic condition, the system disclosed by the invention can realize efficient denitrification, avoid nitrite accumulation, remarkably inhibit generation of nitrous oxide (N2O), reduce dependence on an external organic carbon source and an electron source, and improve the denitrification efficiency. And a new technical approach is provided for improving the deep denitrification efficiency under the low-carbon condition.

The invention provides staphylococcus carnosus with high salt tolerance and good fermentation characteristic and application thereof, and belongs to the technical field of microorganism application and meat product processing. The staphylococcus carnosus is staphylococcus carnosus XUCSB 022, the staphylococcus carnosus is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is June 24, 2025, and the preservation number of the strain is CGMCC No.34991. The staphylococcus carnosus has the advantages that the staphylococcus carnosus XUCSB 022 can be used for preparing the staphylococcus carnosus; the staphylococcus carnosus XUCSB 022 strain disclosed by the invention has the beneficial effects that the staphylococcus carnosus XUCSB 022 strain has the capacity of resisting the salt concentration of 18% ultimately and has the capacity of degrading nitrite in fermented meat, after the meat product is fermented for 15 days, the average value of the residual quantity of the nitrite is 9.77 mg / kg and is reduced by 39.77% compared with a CK group, flavor substances containing aldehydes, ketones, alcohols, esters, acids and other types are generated, and the flavor substances are rich in flavor substances, rich in flavor substances and capable of resisting the salt concentration of 18%. The strain has the characteristics of high safety, excellent fermentation capability and fermentation aroma-producing capability during inoculation of fermented meat, and provides strain resources for production of fermented meat products.

Disclosed is a process for the synthesis of mono- and bis-nitrosylated propanediols, as well as compositions and pharmaceutical formulations that includes the compounds. The process proceeds by reacting a corresponding propanediol that is not nitrosylated with a source of nitrite, optionally in the presence of a suitable acid. When the source of nitrite is an organic nitrite, reacting step is performed in a suitable organic solvent, and when the source of nitrite is an inorganic nitrite, the reacting step is performed in a bi-phasic solvent mixture comprising an aqueous phase and a non-aqueous phase. Also disclosed are methods of treating a condition wherein administration of nitric oxide (NO) has a beneficial effect by administering said compounds, compositions or formulations.

The present invention relates to a method for treating landfill leachate. The steps include: mixing nitrogen-containing landfill leachate and activated sludge and then introducing the mixture into a denitrification tank. Under the condition of inhibiting nitrous oxide reduction, nitrite in the mixture generates nitrous oxide gas dissolved in the sewage under the action of denitrifying bacteria in the denitrification tank; then introducing it into an ammonification tank for gas-liquid separation to collect nitrous oxide gas; and obtaining the sewage after ammonification treatment under the ammonification action of ammonifying functional bacteria in the ammonification tank; further introducing it into a nitrification tank to obtain sewage containing nitrite under the action of nitrosifying bacteria; refluxing to the denitrification tank for cyclic reaction; and then introducing it into a separator for separation to obtain an effluent that can be discharged and sewage that needs to be recycled for treatment. The present invention also relates to a treatment system capable of cooperating with the above treatment method. It changes the existing treatment process of directly discharging nitrogen into the air, turns to collecting nitrous oxide gas, shortens the treatment time, reduces greenhouse gas emissions, and realizes energy recovery.

The invention discloses an electrocatalytic biological denitrification system and denitrification method of a ruthenium-plated electrode composite hollow fiber membrane, and is characterized in that the system comprises a positive / negative electrode chamber arranged on a double-chamber UASB reactor, and a composite biological electrode in the chamber, the composite biological electrode adopts a ruthenium-plated iridium-titanium mesh barrel with a carbon felt wrapped on the periphery as a negative electrode, and the ruthenium-plated iridium-titanium mesh barrel is coated with a hollow fiber membrane. A hollow fiber membrane separation sludge is arranged in the barrel; a ruthenium-iridium-plated titanium mesh is coated with a carbon felt to serve as an anode, electroactive bacteria produce nitrogen / nitrate through anaerobic ammonia oxidation, ammonia nitrogen is electrochemically oxidized into nitrite, anode nitrogen enters a cathode to strengthen mass transfer, cathode nitrite flows back to the anode to be recycled, and efficient conversion and removal of nitrogen are achieved. Compared with the prior art, the system has the advantages of efficient conversion and removal of nitrogen, the pollution of the hollow fiber membrane is reduced and the membrane loss is reduced by utilizing the N2 synthesized by degrading the pre-degradation matrix through the electroactive microorganisms and the additional electrochemical action of the two electrodes, and the system is simple in structure and convenient to maintain and has a good application prospect.

To provide improved beneficial bacteria that can suppress the growth of pathogens.SOLUTION: The present disclosure provides, inter alia, an optimized strain of Nitrosomonas eutropha (N. eutropha) designated D23, D23-100, or AOB D23-100. N. eutropha bacteria disclosed in the present application have desirable properties, e.g., optimized properties, such as the ability to suppress growth of pathogenic bacteria, and an enhanced ability to produce nitric oxide and nitric oxide precursors. The N. eutropha herein may be used, for instance, to treat diseases associated with low nitrite levels, skin diseases, and diseases caused by pathogenic bacteria.SELECTED DRAWING: Figure 18

The present invention provides a synthesis method and application of high-value nitrogen-containing compounds by photocatalytic reduction of nitrogen-containing small molecules. The synthesis method comprises the following steps: mixing a photocatalyst, a nitrogen-containing small molecule, and an electron donor, adding or not adding a co-catalyst, preparing a reaction solution, introducing an inert gas into the reaction solution and sealing it, and irradiating the reaction solution with visible light to obtain the high-value nitrogen-containing compound; wherein the nitrogen-containing small molecule comprises one or more of N2, NO, N2O, nitrate, and nitrite; and the photocatalyst comprises one or more of CdS, CdSe, ZnSe, CuInS2, CdSe / ZnS, CdSe / CdS, ZnSe / CdS, CdSe / ZnSe, CdS / ZnS, CdSe / CdS / ZnS, CdS / TiO2, and CdSe / TiO2. This synthesis method can utilize nitrogen-containing small molecules for efficient reduction to obtain high-value nitrogen-containing compounds such as NH3, urea or cyclohexanone oxime, and has high synthetic application value. At the same time, this synthesis method has mild reaction conditions, high visible light utilization rate, cheap and easily available raw materials, and strong applicability.

The invention relates to the technical field of electro-catalysis, in particular to a metal monatomic doped copper-based catalyst and a preparation method and application thereof. The metal single atoms in the catalyst are selected from palladium single atoms, iron single atoms, nickel single atoms or ruthenium single atoms; the doping amount of the metal single atoms is 0.5%-1.0% on the basis of the total mass of the catalyst. The invention provides a novel metal monatomic doped copper-based catalyst, which can be used for catalyzing the reduction of a harmful substance nitrite into a chemical ammonia with an economic added value by using renewable electric power, can solve the problems of nitrite pollution and environment and energy caused by ammonia production by a Have-Bosch method, and realizes the purpose of turning waste into wealth.

The invention discloses an indium-doped cobaltosic oxide catalyst as well as a preparation method and application thereof, and the indium-doped cobaltosic oxide catalyst is prepared according to the following method: 1) dissolving cobalt nitrate, urea and ammonium chloride in deionized water, and stirring to obtain a clear precipitate-free reaction solution; (2) transferring the reaction liquid into a reaction container filled with foamed nickel, and carrying out hydrothermal reaction to obtain the foamed nickel loaded with a sample; 3) washing the foamed nickel loaded with the sample after the reaction with ethanol and water, and drying; 4, the dried foam nickel loaded with the sample is soaked in an indium chloride solution, stirring reaction is conducted, vacuum drying is conducted, and indium-doped cobaltosic oxide is obtained.By means of indium doping, the morphology and components of the indium-doped cobaltosic oxide are regulated and controlled, and the activity and selectivity of the catalyst for electrochemical reduction of nitrite to produce ammonia are improved.

The invention discloses a preparation method and application of a ruthenium-silver-based catalyst, the prepared catalyst constructs a porous microenvironment, a pore confinement silver site is utilized to adsorb nitrite generated by nitric acid conversion, a high-concentration nitrite local environment is further formed, a ruthenium site is responsible for catalytic conversion of nitrite, and the ruthenium site is responsible for catalytic conversion of nitrite. And finally, energy-saving and high-efficiency ammonia preparation is realized.

The application belongs to the technical field of electrocatalysis, and discloses a bimetallic fluorinated metal organic framework catalyst, a preparation method thereof and application of the catalyst in active oxygen assisted membrane-free electrolytic ammonia oxidation for preparing nitrite, wherein the catalyst is prepared by self-assembly of Cu 2+ , Ni 2+ and a tetrakisfluorophthalic acid ligand through a solvothermal method, and there is a directional electron transfer effect between Cu and Ni. The application utilizes the Cu / Ni bimetallic electron synergistic effect to precisely regulate the d-band center of the catalyst, and a membrane-free electrolysis device is matched to save an expensive proton exchange membrane. The application provides a new efficient and feasible path for green, economical and scaled electrocatalytic synthesis of nitrite.

The invention relates to the technical field of biological metallurgy, in particular to an enhanced leaching method of jorusite. The enhanced leaching method of the jorlybdenum ore comprises the following steps: mixing a liquid phase system containing Fe < 2 + > and a bacterial liquid system formed by acidophilic thiobacillus ferrooxidans with nitrite to obtain a mixed system; then mixing with jorlybdenum ore to obtain a first system; adjusting the pH value of the first system by using an acid solution, and then carrying out oscillation treatment; the concentration of the nitrite in the mixed system is 40-60 mmol / L. According to the enhanced leaching method of the jorlybdenum ore, the complex metabolic process of acidophilic thiobacillus ferrooxidans is combined with nitrite enhanced biological leaching, nitric acid generated by metabolism of the acidophilic thiobacillus ferrooxidans provides acid for molybdenum dissolution of the jorlybdenum ore, generated Fe < 3 + > can be subjected to oxidation-reduction reaction with MoS2 to form stable complex ions [MoO4] < 2->, and the stable complex ions [MoO4] < 2-> are subjected to oxidation-reduction reaction with MoS2. The nitric acid can reinforce the dissolution and leaching of Mo from mineral jorlybdenum ore, so that the leaching effect of Mo is improved.

This invention belongs to the field of electrochemical sensor technology for water quality analysis, specifically relating to an integrated sensor for rapid on-site self-separation detection of nitrite in complex seawater. It includes a self-separating membrane, a membrane slot, a screen-printed electrode, and an electrode slot. Two self-separating membranes fixed in the membrane slots and the screen-printed electrode fixed in the electrode slots are connected sequentially in a vertical direction, which is the sample flow direction. The screen-printed electrode is modified with ZnS / VMSF-TMAC material to form a sensing interface with anion-selective permeability. A filtrate storage cavity is formed between the self-separating membrane and the screen-printed electrode to store the filtrate to be tested after filtration through the self-separating membrane. This sensor, when used in complex seawater environments, has a detection range of 0.005-5 mM and retains 85% of the signal after 28 days of storage.

The present invention relates to a liquid nitric oxide releasing solution (NORS) comprised of at least one nitric oxide releasing compound and at least one acidifying agent, wherein the NORS provides an extended release of a therapeutically effective amount of nitric oxide gas (gNO). The present invention also relates to a liquid NORS comprised of at least one nitrite compound having a concentration of no greater than about 0.5% w / v and at least one acidifying agent, wherein the NORS releases a therapeutically effective amount of gNO. The present invention also relates to a method for the treatment of a wound in a human, the method comprising administering to the human a liquid NORS comprised of at least one nitric oxide releasing compound and at least one acidifying agent, wherein the NORS provides an extended release of a therapeutically effective amount of gNO. The present invention also relates to a method for the treatment, prevention, or reduction of incidence of a disease or disorder in a human in need thereof, the method comprising administering to the human a liquid NORS comprised of at least one nitrite compound at a concentration of no greater than about 0.5% w / v and at least one acidifying agent, wherein the NORS releases a therapeutically effective amount of gNO.

The invention relates to a surface modified Fe / Cu-Co3O4 catalyst for nitrite detection and a preparation method thereof, a sensor and application, and the preparation method comprises the following steps: dissolving a cobalt source, an iron source, a copper source, urea and NH4F in deionized water, carrying out a hydrothermal reaction, washing, drying and calcining to obtain a Fe and Cu co-doped Co3O4 nanotube; and pre-hydrolyzing by using a KH792 silane coupling agent ethanol-water solution, and reacting with nanotubes to prepare the surface modified catalyst. The sensor takes carbon cloth or a screen-printed electrode subjected to ultrasonic cleaning and plasma treatment as a substrate, catalyst ink is coated to form a catalytic layer, and the catalytic layer, a platinum counter electrode and a silver / silver chloride reference electrode form a three-electrode system. Through molecular modification and material design, the interfacial force between the catalyst and nitrite is improved, the electrode stability is enhanced, and the catalyst is applied to a disposable printed electrode and portable detection equipment, so that high-sensitivity, stable and portable detection of nitrite is realized.

This utility model discloses an aerobic mixed liquor nitrification and deoxygenation treatment device, including a first nitrification tank. A nitrite-containing bacteria storage tank is located at the top of the first nitrification tank. An inlet pipe is located at the top of one side of the first nitrification tank, and one side of the inlet pipe extends into the interior of the first nitrification tank and is equipped with a sludge-water separation chamber. Through the cooperation of the inlet pipe, sludge discharge pipe, screen, and sludge-water separation chamber, this utility model can separate the sludge mixed in with the mixed liquor during its inflow, while also facilitating the return of the sludge to the treatment tank. Then, through the cooperation of a second discharge pipe, a third pump, a pump installation chamber, a distribution plate, a distribution pipe, a heat dissipation chamber, and a fan, the mixed liquor can be continuously circulated and cooled during the nitrification reaction, thereby maintaining the mixed liquor at 20-30°C to preserve the activity of nitrite-containing bacteria and nitrifying bacteria in the mixed liquor and ensure reaction efficiency.

Provided herein is a pharmaceutical composition comprising cytisine and a nitrite scavenger, wherein, upon storage of the composition at about 60 ºC, the amount of N-nitrosocytisine relative to a total weight of cytisine is 4.2 ng / mg or less after about 30 days. A method of manufacturing a cytisine tablet is also provided.

The invention relates to a nitrite detection reagent, a nitrite detection method and application. The nitrite detection reagent comprises TMB and iron alkoxide. In the nitrite detection reagent provided by the invention, colorless TMB reacts with oxygen by taking IA as a catalyst, so that an oxidation product TMB < + > of the TMB is formed, and the TMB < + > is blue; on the basis, when the TMB < + > is in contact with the nitrite, the TMB < + > reacts with the nitrite, so that a diazotization reaction is carried out, and green diazotized TMB < + > is formed. Therefore, the nitrite detection reagent disclosed by the invention can realize detection of nitrite by observing the color change of the nitrite detection reagent through naked eyes under sunlight irradiation. The detection mode does not need to depend on instruments, and low-cost rapid detection of nitrite can be realized.

A polymer comprising pyridinium salt moieties, wherein the polymer has the following structure: In this structure, R1 and R6 are each N. At least a portion of R2 and R7 groups in the polymer are each negatively charged counterions selected from the group consisting of carboxylates, nitrates, nitrites, azides, metasilicates, tetraborate, perborates, boronates, oxalatob orates, metaborates, phosphorus-containing anions, halides, sulfates, SO4 -2, HSO4 -1, thiosulfate, dithionite, metabisulfite, persulfate, methanesulfonate, dioctyl sulfosuccinate, 3-sulfopropyl ethoxylate laurylphenyl ether, and combinations thereof. R3, R4, R8, R9, R11, R13, R14, R15, and R16 are each selected from the group consisting of H and groups including one or more carbon atoms. R5 and R10 are any group including one or more carbon atoms. The n is an integer from 2 to about 100,000.

The invention discloses a method for treating aged landfill leachate by ozonation and short-cut denitrification-anaerobic ammonia oxidation, which comprises the following steps: treating the aged landfill leachate by an ozonation unit, and then treating the aged landfill leachate by a short-cut denitrification-anaerobic ammonia oxidation (PDA) biological treatment unit, so that the aged landfill leachate can be discharged after reaching the standard. The ozone oxidation unit is used for continuously and efficiently cracking macromolecular refractory organic matters such as humic acid in the aged leachate through ozone, so that the biodegradability is remarkably improved; and the nitrogen proportion is synchronously optimized to form an ideal PDA flora substrate. According to the PDA biological treatment unit, mixed sludge of anaerobic ammonia oxidation sludge and denitrifying bacteria sludge is inoculated in a UASB reactor, and after gradual domestication, short-cut denitrification activity in a PDA biological treatment system is remarkably higher than nitrite reduction activity, and continuous supply of nitrite is guaranteed; synergistic metabolism of anaerobic ammonium oxidation bacteria and denitrifying bacteria is achieved, and the problem of organic matter inhibition is solved. The process and the device provided by the invention provide a green and efficient innovative solution for degradation-resistant wastewater with high ammonia nitrogen and low carbon nitrogen ratio.

The invention belongs to the technical field of material chemistry, and particularly relates to an MOF catalyst containing high-valence copper and a preparation method and application of the MOF catalyst. According to the invention, Ag is doped on a Cu-based metal organic framework material framework through an ion exchange method, then the Ag is mixed with a binder solution to prepare slurry, the slurry is coated on an electrode substrate, the electrode substrate is dried to serve as a working electrode, constant potential or circulating potential is applied to the working electrode in a preset voltage range in an electrochemical system containing an electrolyte, and thus the Cu-based metal organic framework material is obtained. Cu (III) is generated in situ through electrochemical reconstruction, and the MOF catalyst containing high-valence copper is obtained. The MOF catalyst containing high-valence copper has excellent ammoxidation reaction activity and stability, is especially suitable for high-conversion-rate and high-selectivity ammoxidation reaction to prepare nitrite, and is suitable for industrial induced production application.

The invention discloses a method for treating industrial wastewater rich in high-concentration nitric acid, and belongs to the technical field of range, and in the method, the treatment efficiency of high-concentration nitric acid and ammonia nitrogen wastewater is remarkably improved through the synergistic effect of catalytic oxidation and biological denitrification. In the catalytic oxidation stage, a Fe-Cu bimetallic catalyst is adopted to directionally regulate and control the nitrogen form, nitrate is reduced while part of ammonia nitrogen is oxidized into nitrite, nitrosation accumulation is formed, and the follow-up biological treatment load is greatly reduced. In the biological denitrification stage, through a short-cut denitrification and anaerobic ammonia oxidation coupling path, ammonia is directly used as an electron donor to react with nitrite derivatives to generate nitrogen, not only is the carbon source demand reduced by 60% or above, but also the sludge yield is reduced by 40%, the comprehensive operation cost is saved by 50% or above compared with that of a traditional process, and no secondary pollutants are generated in the whole process.

The present invention relates to systems and methods for catalytic removal of oxidized contaminants (for example, nitrite, chromate, chlorate, trichloroethene, CFC-11, 4-nonylphenol, RDX, and perfluorooctanoate) from water and wastewater. In some aspects, the catalytic method of removing oxidized contaminants comprises using precious metal nanoparticles as catalysts to reduce the oxidized contaminants.

This invention discloses a GSH-functionalized carbon dots / rhodamine B ratiometric fluorescence system, its construction method, and its application in nitrite detection. GSH-CDs are synthesized via a one-step hydrothermal method by mixing citric acid, o-phenylenediamine, glutathione, and hydrochloric acid in water. Unreacted precursors and small molecules are removed by purification, and the GSH-CDs are obtained by freeze-drying. The ratiometric fluorescence system is constructed by mixing rhodamine B, GSH-CDs, and an acidic buffer solution. The fluorescence intensity ratio F of GSH-CDs to rhodamine B is established. 460 / F 590 With nitrite (NO2) ‑ A calibration curve between concentrations was obtained, and the NO2 in the actual sample was experimentally detected using the spiked recovery method. ‑ Content. Under acidic conditions, with NO2... ‑ With increasing concentration, the GSH-CDs / RhB fluorescence intensity ratio (I 460 / I 590 Significant changes occurred, with the fluorescence intensity ratio compared to NO2. ‑ There is a good linear relationship between concentrations. Compared with the detection platform based on GSH-CDs, the ratio fluorescence system provided by this invention has a lower detection limit and significantly higher sensitivity.

Provided are wound dressings for treating wounds, which include a nitric oxide generating layer for generating nitric oxide by acidification of nitrite, wherein the nitric oxide generating layer includes a solid powder nitrite component and a solid proton source component, as well as methods and uses of such wound dressings for treating wounds.

The invention discloses a whole-process denitrifying pseudomonas and application thereof in textile printing and dyeing sewage, and relates to the field of environmental microorganism water environment governance, the key points of the technical scheme are as follows: the whole-process denitrifying pseudomonas is pseudomonas stutzeri S8 and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is June 23, 2025, and the preservation number is CCTCC NO: M 20251451. The Pseudomonas stutzeri S8 can simultaneously perform whole-course denitrification and decolorization under the toxicity stress of hexavalent chromium ions, the removal rate of nitrate and nitrite in chromium-containing actual textile printing and dyeing sewage can be higher than 95% after standing culture is performed for 48 hours, the synchronous dye removal rate is higher than 82%, the tolerant nitrate and nitrite concentration is higher than 500 mg / L, and the application range is wide. The strain has the advantages that the concentration of hexavalent chromium ions tolerated by the strain is higher than 50mg / L, in addition, the strain also has good decoloring and denitrification effects in a decoloring and denitrification culture medium stressed by other five single dyes, the nitrate removal rate is higher than 98%, and the dye removal rate is 70%-100%.

A polymer comprising pyridinium salt moieties, wherein the polymer has the following structure: In this structure, R1 and R6 are each N. At least a portion of R2 and R7 groups in the polymer are each negatively charged counterions selected from the group consisting of carboxylates, nitrates, nitrites, azides, metasilicates, tetraborate, perborates, boronates, oxalatob orates, metaborates, phosphorus-containing anions, halides, sulfates, SO4 -2, HSO4 -1, thiosulfate, dithionite, metabisulfite, persulfate, methanesulfonate, dioctyl sulfosuccinate, 3-sulfopropyl ethoxylate laurylphenyl ether, and combinations thereof. R3, R4, R8, R9, R11, R13, R14, R15, and R16 are each selected from the group consisting of H and groups including one or more carbon atoms. R5 and R10 are any group including one or more carbon atoms. The n is an integer from 2 to about 100,000.

A method of fabricating a plant-based additive for inhibiting nitrite formation during meat preparation includes reacting a pomegranate peel extract with a zinc precursor solution to obtain a reaction mixture, stirring the reaction mixture for 15 to 60 min at a temperature of 50 to 90° C. to obtain zinc oxide nanoparticles; and combining the zinc oxide nanoparticles with at least one extract selected from the group consisting of red cabbage extract and a beet root extract to obtain the plant-based additive. The extract comprises 500 to 1000 parts per million of nitrate relative to a total weight of the plant-based additive.