Lactobacillus plantarum strain having functions of effectively degrading nitrite and strongly producing acid and application of lactobacillus plantarum strain
A technology of Lactobacillus plantarum and nitrite, applied in the field of new strains of Lactobacillus plantarum, can solve the problems of difficult product quality control, high nitrite content, low productivity, etc., and achieves excellent fermentation performance and osmotic pressure resistance, sub- The effect of low nitrate content and strong acid production capacity
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[0028] 1. Primary screening of strains
[0029] Vegetable juice was fermented from traditional fermented pickles made by families in Nanjing and traditional fermented pickles made by farmers in Shenyang, and the samples were serially diluted with sterile water (10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 ), take 100ul of the four gradient dilution samples and evenly spread them on the MRS agar medium, do 3 parallels for each gradient, culture at a constant temperature of 30°C for 48h, select a single colony with typical lactic acid bacteria colony morphology and streak it on the MRS plate Purification, passage 3-4 times, after microscopic examination as pure species, combined with colony morphology, Gram staining and contact enzyme test, 23 strains of lactic acid bacteria were obtained through preliminary screening, which were numbered FM-LP1-1 and FM-LP1 in sequence -2, FM-LP1-3, FM-LP1-4, FM-LP1-5, FM-LP1-6, FM-LP1-7, FM-LP1-8, FM-LP1-9, FM-LP2-1 , FM-LP2-2, FM-LP2-3, FM-...
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