4404 results about "Lactobacillus plantarum" patented technology

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

The invention relates to a lactobacillus plantarum strain having functions of effectively degrading nitrite and strongly producing acid and an application of the lactobacillus plantarum strain, and belongs to the technical field of bioengineering. The lactobacillus plantarum strain having the functions of effectively degrading nitrite and strongly producing acid is selected from conventional fermented pickled vegetables in Shenyang peasant families, wherein the strain is named FM-LP1-1, identified to be lactobacillus plantarum, and the strain is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No. 9488 on August 4, 2014. By taking FM-LP1-1 as a fermenting agent for fermenting vegetables, the content of nitrate in the fermented vegetable products is effectively reduced, the fermenting duration of the vegetables is shortened and the fermenting flavor of the vegetables is enhanced; and the strain disclosed by the invention is broad in application prospect.

The invention relates to a drink, in particular to a probiotic type fruit and vegetable enzyme solid drink. The probiotic type fruit and vegetable enzyme solid drink is prepared from raw materials in parts by mass as follows: 10-50 parts of prebiotics, 5-40 parts of water-soluble dietary fiber, 0-20 parts of sugar alcohol, 0.1-15 parts of fruit powder, 0.1-10 parts of fruit and vegetable enzyme powder, 0.01-15 parts of freeze-dried bifidobacterium infantis powder, 0.01-20 parts of freeze-dried bifidobacterium breve powder, 0.01-30 parts of freeze-dried Lactobacillus plantarum powder, 0.01-25 parts of freeze-dried bifidobacterium longum powder and 0.01-20 parts of freeze-dried Lactobacillus casei powder. The probiotic type fruit and vegetable enzyme solid drink adopts the natural raw materials and the scientific formula and is balanced in nutrition and beneficial to intestinal health and body immunity.

The invention discloses an organic matter decomposing inoculant, and a preparation method and application thereof. The organic matter decomposing inoculant comprises the following ingredients in part by weight: 10 to 20 parts of aspergillus niger microbial inoculum, 10 to 20 parts of lactobacillus plantarum microbial inoculum, 20 to 30 parts of saccharomyces cerevisiae microbial inoculum and 40 to 60 parts of bacillus subtilis microbial inoculum. The organic matter decomposing inoculant mainly comprises various microbial floras with mutual promotion and mutual synergy effects. The organic matter decomposing inoculant can decompose various organic matters quickly, can effectively kill germs, ova and weed seeds in the decomposed raw materials, can reduce diseases, weed and insect pests, andcan remove water and odor. The organic matter decomposing inoculant can stimulate growth and development of crops, can improve disease resistance, drought resistance and cold resistance, can increasesoil nutrients, can improve the soil structure, and can increase the utilization ratio of chemical fertilizer. The organic matter decomposing inoculant can be used directly and can also be used for producing commercial organic fertilizer, biological organic fertilizer, organic and inorganic complex fertilizer, biological organic and inorganic complex fertilizer and the like.

The present invention discloses a fruit and vegetable probiotic tablet and a preparation method thereof. The fruit and vegetable probiotic tablet uses probiotic powders such as lactobacillus plantarum powder as the main raw material, and the preparation method scientifically mixes modified dietary fibers, fruit and vegetable powder, oligosaccharides, plant extracts, protein powder, tea leaf extracts and traditional Chinese medicine extracts and etc., thus improves the content of soluble celluloses which are of real significance for probiotic flora, enhances the physiological activity of celluloses, thereby increases the species of intestinal probiotic flora as well as significantly enhances the colonization ability and time of endogenous and exogenous probiotics in the human intestinal tracts, effectively inhibits the growth and reproduction of harmful intestinal bacteria, especially gram-negative bacteria, and fully regulates the composition of the intestinal probiotic flora. The prepared fruit and vegetable probiotic tablet has a high biological activity, a long human intestinal colonization time, and a significant weight loss effect, and is suitable for a wide range of people.

The invention relates to a method for preparing health-benefiting fermented soybean meal enriched in small peptides, gamma-aminobutyric acid (GABA), reducing sugar, lactobacillus plantarum and antimicrobial peptide. By utilizing strains of aspergillus oryzae, saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum, the small peptides, free amino acids, reducing sugar, GABA and high viable count content in the lactobacillus plantarum in the health-benefiting fermented soybean meal are obtained through the two-stage solid state fermentation method, the small peptides account for 8.01-18 percent, the free amino acids has the content of 80-300mg/g, the reducing sugar reaches 20-80mg/g, the GABA reaches 0.317-0.5mg/g, the viable count content in the lactobacillus plantarum is 2-6.5*1010cfu/g, the antibacterial titer of antimicrobial peptides is 2.0*103-7.0*104 IU/g, and the yield of the health-benefiting fermented soybean meal is 82.03-90 percent. According to the process, the nutrient effect of the soybean meal can be improved, the prepared fermented soybean meal has the antibacterial effect, partial antibiotics can be replaced, the fermented soybean meal is conveniently preserved and saved, the process is simple and feasible, and the fermented soybean meal is green and environment-friendly and has application significance and wide prospect in the feed industry and breeding industry.

The invention provides a feed fermenting agent, fermented feed and a preparation method thereof, and relates to the technical field of animal product cultivation. The feed fermenting agent is mainly prepared from lactobacillus plantarum, enterococcus faecalis, bacillus subtilis, saccharomyces cerevisiae, lactobacillus cellobiosas, a complex enzyme preparation and the like. The fermented feed is prepared from the feed fermenting agent and fermentation raw materials by using a preparation method of carrying out fermentation in a device which is in one-way exhaust. According to the feed fermenting agent and the fermented feed, disclosed by the invention, the problems of high livestock cultivation cost caused by poor nutritive value, low animal digestion and absorption rate and abuse of antibiotic drugs during cultivation, long fermentation time and slow fermentation starting of existing livestock breeding feed are solved, and high absorption and conversion rate of nutrient contents of the fermented feed are achieved; the fermented feed contains a large amount of probiotics for protecting intestine health of animals, so that the organism immunity of the animals is increased, and the use of antibiotics during livestock cultivation is reduced.

The invention discloses a denitrifying strain with nitrifying function, a strain-containing water body improver of multiple active microorganisms and a preparation method of the water body improver, belonging to the technical fields of aquaculture and water body improvement. The strain disclosed by the invention is conserved in the China center for type culture collection (CCTCC), the CCTCC NO isM2011443; and the water body improver of the active microorganisms which is based on the strain comprises multiple strains such as Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus cereus, Bacillus megaterium, Rhodopeudomonas palustris, Arthrobacter globiformis, Rhodotorula rubra and Lactobacillus plantarum. The strain is used to perform liquid fermentation and obtain fermentation liquor of which number of live bacteria is 0.5-3*10<10>cfu/mL, bacterial powder of which the number of live bacteria is 0.2-2*10<12>cfu/mL is further prepared; and the bacterial powder is used along with a diluent to prepare a liquid or powdery water body improver, wherein the effective live bacteria of the water body improver is 0.1-3*10<10>cfu/mL(or cfu/g). The water body improver has the function of reducing the contents of ammonia nitrogen, nitrites and nitrates in the culture water body; and the water body improver is suitable for different culture water bodies and has the function of improving the water quality.

The invention relates to lactobacillus plantarum CCFM8724 and application thereof. The lactobacillus plantarum CCFM8724 has ability of suppressing growth of campylobacter jejuni in vitro, good resistance to acid and cholate and good adhesive ability to intestinal epithelial cells, and can control the campylobacter jejuni adhering to the intestinal epithelial cells so as to suppress growth of the campylobacter jejuni in chicken. The lactobacillus plantarum CCFM8724 can be used for preparing a medicinal composition for suppressing campylobacter jejuni; a fermentation agent containing the strain of lactobacillus plantarum CCFM8724 can be used for producing fermented dairy products, fermented bean products, fermented fruit/vegetable products and silage; the fermentation agent enables the products to obtain certain acidity and particular flavor; and meanwhile, the product preserving time is prolonged, and the nutritional value, digestibility and safety of the products are improved.

The invention discloses a method for producing a complex micro-ecological preparation with microbial agents and enzyme. According to the action principles and characteristics of every microbial strain, the method adopts scientific combination and adds enzyme preparations and acidifier to enable microorganisms in the product to grow rapidly and take dominance in fermented feed and animal bodies so as to reduce the growth opportunity of miscellaneous bacteria. The preparation comprises the following components: bacillus subtilis, lactobacillus plantarum, candida utilis yeast, lactobacillus acidophilus, protease, amylase, cellulase, xylanase, mannanase, galactosidase, pectinase and citric acid. As all strains of the product adopt liquid deep fermentation culture, the non-polluting property of the product is guaranteed. Liquid microbial agents ensure that the number of bacterial colonies of the product is more than 100 million and the product is free from the pollution of other miscellaneous bacteria, while solid microbial agents ensure that the number of bacterial colonies of the product is more than 1 billion and the proportion of miscellaneous bacteria to effective bacteria is not more than 0.0001. The preparation contains bacillus, lactic acid bacteria and yeast, can apply to the processes of making green-yellow storage feed, fermenting feed, soaking feed, and the like, can be directly added to feed for feeding, and can improve the growth performance of ruminant animals, poultry, pigs, aquatic products and special animals.

The invention relates to a complex microbial inoculum 707 and a preparation method and application thereof, and belongs to the technical field of biological materials of the application of plant biological regulators to crop planting. The preparation method of the complex microbial inoculum 707 comprises the following step of: performing the cultivation of one-level strains and second-level and third-level fermentation on strains comprising 45 to 55 percent of bacillus subtilis, 5 to 10 percent of bacillus licheniformis, 5 to 10 percent of bacillus megaterium, 5 to 10 percent of candida utilis, 5 to 10 percent of rhizobium japonicum, 5 to 10 percent of bacillus mucilaginosus and 5 to 10 percent of lactobacillus plantarum, so that the microbial content of fermentation liquor reaches 6-10*10<9> per milliliter to obtain the complex microbial inoculum 707. Through long-term field verification, the complex microbial inoculum 707 has the advantages of reasonable formula and stable effect, can promote the growth of crops, improve fruit quality, promote early ripening, increase yield, improve the utilization rate of fertilizers and improve the ecological environment of soil, and has certain effect on disease prevention and disease resistance.

The invention discloses lactobacillus used for biological preservation, which is named Lactobacillus plantarum and has been collected in China Center for Type Culture Collection on June 21, 2010 with the collection number of CCTCC M 2010150. The lactobacillus can be used for fruit preservation. The application method comprises the following steps: heating and dissolving an acidizer in water, reducing temperature to normal temperature, adding lactobacillus powder, uniformly mixing, spraying onto fruits in an atomized mode, and storing the fruits at normal temperature or low temperature. Under the condition of normal temperature, the fruits can be preserved for 3 months, and under the condition of low temperature, the fruits can be preserved for 6 months. The Lactobacillus plantarum is obtained by screening a large amount of lactobacillus, can control peel browning and reduce fruit morbidity, and also can properly reduce fruit moisture violation, inhibit the metabolism of the fruits, and prolong the refreshing time.

Lactobacillus plantarum having an anti-diabetic function and application thereof are disclosed, the strain is Lactobacillus plantarum NCU116, and the accession number is CGMCC 11885. The strain has a function to alleviate symptoms of diabetes, and the composition is in a form of food or a pharmaceutical composition. The present invention also discloses a method for screening and identification of the lactic acid bacteria strain as well as a method to achieve the anti-diabetic effect by regulating blood sugar, blood lipids and hormone levels and regulating metabolic pathways in the body. The daily-intake lactic acid bacteria or a composition thereof is used to replace a medicine to achieve the anti-diabetic effect; and the healthy daily-intake lactic acid bacteria or the composition thereof is free of toxic and side effects to human body is used as a new choice to alleviate the symptoms of diabetes.

The invention discloses a method to prepare leaven of fertilizer and application, which is characterized by the following: allocating raw material with mass ratio at 10-80 wt industrial residue of ferment, 5-30 wt straw, 5-30 wt cake meal, 10-30 wt bran or rice bran, 1.5-2 wt aquacare, 0.5-1 wt NaCl and or amylaceum, and 1-30 wt brown ball Azotobacter (Azotobacter chroococcum), 1-30 wt bacillus megaterium (Bacillus megaterium), 1-30 wt bacillus licheniformis (Bacillus licheniformis), 1-30 wt plant lactobacillus (Lactobacillus plantarum) and 1-30 wt 1-5 strain of saccharomycopsis fibuligera (Saccharomycopsis fibuligera); producing the product.

The invention relates to an early weaning meat sheep fattening compound biological feed additive, which belongs to the technical field of animals feeding by feed. The feed additive comprises prebiotics consisting of candida utilis, bacillus subtilis and a lactobacillus plantarum microbial agent, non-starch polysaccharide enzyme containing cellulase, xylanase, beta-dextranase, beta-mannase and pectinase and a yeast culture formed by using saccharomyces cerevisiae to completely ferment bean pulp. The additive is specially used for fattening period ration of the early weaning(the weaning lunar age is less than or equal to 2 ages of the moon) meat sheep, and the adding percentage for the full-mixing ration of the fast-fattening meat sheep in 3 to 5 ages of the moon is 1 percent. The additive can provide functional nutrients such as digestive enzyme, B group vitamins and growth promoting factors, and the like which are inadequately produced and insufficient for the early weaning baby sheep and simultaneously contains probiotics for adjusting the micro-ecological environment in intestinal canals, thereby improving the immunity and the healthy level of the baby meat sheep; and after weaning, the daily gaining in weight is more than 10 percent in the prior period and later period of fattening, and the economic benefits are obviously improved.

A novel strain of Lactobacillus plantarum, referred to as LB931, has now been isolated. The strain has been deposited at Deutsche Sammlung von Mikroorganismen, Braunschweig, DE. It has been assigned accession number DSM11918. Accordingly LB931 can be used for treating and/or preventing urogenital infections. LB931 can advantageously be included in pharmaceutical compositions and in products for personal care, such as feminine hygiene products, diapers and sanitary napkins.

The invention relates to a feeding addition agent for microorganism viable bacteria and a preparing method thereof. The addition agent consists of 1.5-2.5 parts of bacillus coagulans viable bacteria powder, 0.5-2.0 parts of lactobacillus acidophilus viable bacteria powder, 1.0-2.5 parts of lactobacillus plantarum viable bacteria powder, 0.5-2.5 parts of bacillus natto viable bacteria powder, 0.5-1.5 parts of candida utili viable bacteria powder and 2-4.5 parts of aspergillus niger viable bacteria powder. Each gram of the content of viable bacteria is as follows: bacillus coagulans is not less than 1.0*10cfu/g; lactobacillus acidophilus is not less than 1.0*10cfu/g; lactobacillus plantarum is not less than 1.0-10cfu/g; bacillus natto is not less than 1.0*10cfu/g; aspergillus niger is not less than 1.0*10cfu/g; and candida utili is not less than 1.0*10cfu/g. The invention has the advantages that the composition is reasonable, the preparation method is scientific, the usage scale is wide, the operation is simple and easy to master, and the cost is low. In addition, the invention can be used as the feeding stuff agent for various poultries, thus increasing the use ratio of the feeding stuff addition agent and strengthening the anti-disease ability of the poultries.

A method for producing probiotic feed for animals by fermenting high-fiber agricultural and sideline products with complex bacteria. , and/or 0-1.0% calcium chloride and/or 0-1.0% magnesium sulfate) are mixed in proportion and then steamed with water; after the steaming is completed, cool to 20-40°C and inoculate Neurostria fortis and Lactobacillus plantarum is solid-state fermented at 20-40°C for 2-5 days; the fermented feed is dried at 40°C-70°C and crushed to obtain a probiotic feed product rich in carotenoids. The method has the advantages of high production efficiency, low cost and good benefit, and while solving the problem of environmental pollution, it also relieves the pressure of serious shortage of energy and protein feed sources.

The invention discloses a lactobacillus plantarum and a preparation method thereof for high-density culture and freeze-drying bacteria powder, and has the technical scheme that the lactobacillus plantarum (Lactobacillus plantarum P8) is probiotics separated from the traditional fermentation milk product (yoghurt) of the Urat Middle Banner of Inner Mongolia Bayan Nur. The probiotics has the characteristics of artificial gastric juice and artificial digestive juice tolerance, cholate tolerance, intestinal agglutination action and common pathogenic entero bacteria inhibition. The bacterial strain of the bacteria is collected in the China general microbiological culture collection center with the collection number of CGMCC (China general microbiological culture collection center) No. 5468 on 18th, Nov., 2011.

The invention discloses lactobacillus plantarum fermented fruit and vegetable juice blends and a preparation method thereof. The lactobacillus plantarum fermented fruit and vegetable juice blends comprises the following raw material components: 10-90 parts of fruit and vegetable original juice, 8 parts of white sugar, 0.1-0.5 part of thickening stabilizing agent and 0.01-0.03 part of compound antioxidant. The invention also provides a preparation method of the lactobacillus plantarum fermented fruit and vegetable juice blends. The lactobacillus plantarum fermented fruit and vegetable juice blends provided by the invention is reasonable in nutrient matching; through screening and fermenting optimal lactobacillus plantarum strains, the content of amino acid in the fruit and vegetable juice is increased, novel vitamins and organic acids are synthesized, aroma constituents are generated, the original bitter and astringent taste of vegetables is improved, nutrients of different fruits and vegetables can be included in one final product so that different nutrient demands of consumers are met; meanwhile, the problem of sediment of the product within a guarantee period is solved through the addition of the thickening stabilizing agent, and the problem of color uniformity of the product within the guarantee period is solved through the addition of the compound antioxidant.

The invention relates to a method for preparing a probiotic preparation for reducing blood lipid and adjusting intestinal flora, which comprises the following steps that: (1) Kefir-source lactobacillus plantarum MA2 is subjected to high-density culture to obtain a fermentation fluid, and the thallus density of the fermentation fluid is not less than 10cfu/mL; (2) the fermentation fluid is subjected to centrifugal collection to obtain thalli, and the thalli are added with a protective agent and are freeze-dried into bacterial powder; and (3) the freeze-dried bacterial powder is added with an oligosaccharide mixture, and a finished product of the probiotic preparation can be obtained after even mixing. The prepared probiotic preparation has a function of reducing the blood lipid, also has a physiological function of adjusting the intestinal flora at the same time, can treat and prevent high blood lipid symptoms, adjust the intestinal flora, is non-toxic and safe, has long retention period, and can be prepared into capsules, micro-capsules, granules, tablets and the like.