Lactobacillus plantarum and preparation method thereof for high-density culture and freeze-drying bacteria powder

A technology of Lactobacillus plantarum and high-density culture, which is applied in the fields of Lactobacillus plantarum and its high-density culture and freeze-dried bacteria powder preparation, can solve the problems of large difference in the highest bacterial cell concentration, no definition of high-density culture, etc., and achieves improvement. Intestinal flora, the effect of strong tolerance

Inactive Publication Date: 2013-01-09
北京和美科盛生物技术有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large difference in the maximum concentration of bacteria that can be achieved by various strains, there is no exact definition of high-density culture in the current literature.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A strain of Lactobacillus plantarum, said plant Lactobacillus (Lactobacillus plantarum P8) is a strain of probiotics isolated from traditional fermented milk products (yogurt) in Urad Zhong Banner, Bayannur City, Inner Mongolia, and has resistance to artificial gastric juice and artificial digestive juice. Tolerance, bile salt tolerance, intestinal agglutination and inhibition of common intestinal pathogenic bacteria and other characteristics; the strain has been preserved in the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee on November 18, 2011 , the deposit number is CGMCC No.5468.

[0039] High density culture method of Lactobacillus plantarum P8:

[0040] a. Activation of strains: inoculate Lactobacillus plantarum P8 frozen at -80°C in 5ml MRS liquid medium (MRS medium: 10g peptone, 10g beef extract, 5g yeast extract powder, 20g glucose, Dipotassium hydrogen phosphate 2g, sodium acetate 5g, trisodium c...

Embodiment 2

[0047] A strain of Lactobacillus plantarum, said plant Lactobacillus (Lactobacillus plantarum P8) is a strain of probiotics isolated from traditional fermented milk products (yogurt) in Urad Zhong Banner, Bayannur City, Inner Mongolia, and has resistance to artificial gastric juice and artificial digestive juice. Tolerance, bile salt tolerance, intestinal agglutination and inhibition of common intestinal pathogenic bacteria and other characteristics; the strain has been preserved in the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee on November 18, 2011 , the deposit number is CGMCC No.5468.

[0048] High density fermentation of Lactobacillus plantarum P8:

[0049] a. Activation of strains: Lactobacillus plantarum P8 frozen at -80°C was inoculated into MRS liquid medium and cultured at 37°C for 18 hours, and then subcultured twice to obtain activated strains.

[0050] b. Prepare optimized medium, sucrose 50-80kg, ...

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Abstract

The invention discloses a lactobacillus plantarum and a preparation method thereof for high-density culture and freeze-drying bacteria powder, and has the technical scheme that the lactobacillus plantarum (Lactobacillus plantarum P8) is probiotics separated from the traditional fermentation milk product (yoghurt) of the Urat Middle Banner of Inner Mongolia Bayan Nur. The probiotics has the characteristics of artificial gastric juice and artificial digestive juice tolerance, cholate tolerance, intestinal agglutination action and common pathogenic entero bacteria inhibition. The bacterial strain of the bacteria is collected in the China general microbiological culture collection center with the collection number of CGMCC (China general microbiological culture collection center) No. 5468 on 18th, Nov., 2011.

Description

technical field [0001] The invention relates to a strain of plant lactobacillus and a method for its high-density cultivation and preparation of freeze-dried bacterial powder. Background technique [0002] Lactobacillus plantarum is closely related to human life. It is a lactic acid bacteria commonly found in butter, meat and many vegetable fermented products. It can pass through the stomach and colonize the intestinal tract to play a beneficial role. It has an important impact on intestinal microorganisms, and has a wide range of applications in food fermentation, industrial lactic acid fermentation, and medical care. [0003] Lactobacillus plantarum P8 used in the present invention is a strain of probiotics isolated from traditional fermented milk products (yogurt) in Urad Middle Banner, Bayannaoer City, Inner Mongolia. Through 16S rDNA homology analysis, it is similar to the standard strain Lactobacillus plantarum in Gene Bank. The homology of ATCC14917 is 100%. As a pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/25
Inventor 高鹏飞姚国强
Owner 北京和美科盛生物技术有限公司
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