48results about How to "Strong tolerance" patented technology

This invention belongs to the field of microbial technology, specifically relating to a strain of Acetobacter peroxide 672 and its applications. The Acetobacter peroxide 672 strain of this invention can enhance the antagonistic activity of Pichia pastoris against Rhizoctonia solani and also antagonizes Colloidal anthracnose, providing a multi-faceted solution for the biological control of crop diseases. Furthermore, the strain possesses the ability to produce indoleacetic acid, which can significantly promote the growth and development of grain crops such as wheat and corn, promote root expansion, enhance nutrient and water absorption, and thus effectively increase biomass. In addition, the strain can tolerate and degrade chemical pesticide components such as imidacloprid and carbendazim, alleviating soil pesticide residue pollution, and can effectively alleviate the inhibitory effects of low pH stress on crops, enhancing their stress resistance. Therefore, the Acetobacter peroxide 672 strain of this invention has significant application value in the biological control of crop diseases, crop growth promotion, soil pesticide residue remediation, and enhancing crop stress resistance.

The invention relates to the technical field of microorganisms and the field of food, in particular to lactobacillus plantarum for inhibiting brown stain of soybean paste and application of lactobacillus plantarum. The lactobacillus plantarum is screened from traditional naturally-fermented peasant soybean paste in northeast China, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 30, 2025, and the preservation number is CGMCC No.35048. The lactobacillus plantarum has an obvious browning inhibition effect and can be widely applied to fermented food production.

This invention belongs to the interdisciplinary field of artificial intelligence and geological resource assessment, specifically relating to a method and system for predicting the energy storage potential of salt caverns based on multi-source geological information. It aims to address the problems of low prediction accuracy and difficulty in balancing breadth and precision in existing technologies due to single-source data, static models, and lack of adaptive capabilities. The method includes: acquiring multi-source data such as seismic, well logging, core, and geostress data; constructing a geological feature fusion vector containing seven core parameters; establishing a high-dimensional nonlinear mapping model driven by a deep neural network; dynamically adjusting the prediction range based on data coverage density and confidence level; and achieving continuous model iteration through online incremental learning. The system integrates data quality assessment, anomaly handling, and distributed computing modules. By adopting the above technical solutions, this application can achieve a significant improvement in prediction accuracy and adaptive intelligent adjustment of the prediction range.

Disclosed are bacterial strain HSCY 2073, isolation and screening thereof, and application thereof in improvement on flavor quality of vinegar. Bacterial strain HSCY 2073 is identified as Lactobacillus sp. and is deposited in China General Microbiological Culture Collection Center, where deposit date is 21 November 2019, and deposit number is CGMCC No. 18982. Bacterial strain HSCY 2073 in the present disclosure is Lactobacillus sp. that has strong acetic acid and ethanol tolerance and excellent effect of producing non-volatile acid, and can produce high contents of 5 aroma substances including acetaldehyde, ethyl acetate, isobutyl acetate, isoamyl acetate and ethyl 3-methylvalerate that have fruit aromas. The lactobacillus can adapt to high-acid, high-alcohol and oligotrophic harsh environments, etc. of vinegar brewing, and has strong ability of high acid tolerance.

The invention discloses a sulfur-tolerant platinum-based catalyst and a preparation method thereof, and the preparation method of the sulfur-tolerant platinum-based catalyst comprises the following steps: weighing roasted ZrO2 as a carrier, weighing ammonium metatungstate, completely dissolving in deionized water, dropwise adding the solution until the carrier is completely immersed, and carrying out ultrasonic treatment; standing at room temperature, drying, and roasting in air at 500-600 DEG C to prepare a tungsten oxide loaded solid acid composite carrier WO3-ZrO2; and weighing chloroplatinic acid, completely dissolving the chloroplatinic acid in deionized water, dropwise adding the solution into the composite carrier, completely immersing the composite carrier, carrying out ultrasonic treatment, standing at room temperature, drying, and roasting in air at 250-350 DEG C to obtain the sulfur-tolerant platinum-based catalyst. The catalyst is simple in preparation method, has relatively strong tolerance to different sulfur species, and has good low-temperature catalytic performance and catalytic stability to low-carbon alkane.

The invention relates to an azo dye degrading bacterium SHY-2 and application thereof, the strain can efficiently degrade azo dye acid red 73 and reactive black 5, in an inorganic salt culture medium only added with glucose, cane sugar, starch or glycerol as a carbon source, the highest degradation rates of 100 mg / L acid red 73 and reactive black 5 within 5 days can reach 98.7% and 95.3% respectively, an organic nitrogen source is not needed, and the treatment cost is low. A mixed flora SH formed by the strain and a serratia bacterium also has a remarkable synergistic degradation effect on two dyes, and complete decolorization can be realized within 48-60 hours. The strain and the flora thereof can be used for biological treatment of azo dye wastewater, and have the advantages of simplicity and convenience in operation, environmental friendliness, great application potential and the like.

The present application relates to the field of microorganism, discloses a kind of fusarium fujikuroi, the fungicide containing the bacteria and their application and the method for producing gibberellin.The preservation number of the fusarium fujikuroi is CCTCC NO:M 20221848.The present application further provides the application of the fusarium fujikuroi in producing gibberellin.The method for producing gibberellin includes the following steps: the fusarium fujikuroi and / or the fungicide is inoculated into seed culture medium to carry out seed culture and obtain seed liquid;The seed liquid is inoculated into fermentation culture medium to carry out fermentation culture.The fusarium fujikuroi provided by the present application can resist gamma ray irradiation, has good environmental adaptability and gibberellin production potential, and is suitable for industrial production.

The invention relates to the field of genetic engineering and fermentation engineering, and particularly discloses a method for exogenous expression of COQ3 by recombinant pichia pastoris engineering bacteria. According to the invention, a target gene COQ2 is inserted into a pichia pastoris secretory expression vector ppicZA to construct a recombinant expression plasmid. The plasmid is transformed into a pichia pastoris GS115 host bacterium to obtain a recombinant pichia pastoris engineering bacterium capable of expressing COQ3. Through methanol-induced fermentation, the engineering bacterium can accumulate COQ3 in cells. The method provided by the invention has the advantages of low cost, easiness in large-scale production and the like, and provides a new way for realizing industrial production of COQ3 and other ubiquinone substances.

The invention belongs to the technical field of biological medicine, and particularly relates to a preparation method of a probiotic hydrogel material and application of the probiotic hydrogel material in treatment of acute radioactive intestinal injury. In order to solve the clinical key problem of radioactive intestinal injury, a novel biological material TG-CCS-coated DY802 of thiolated synchronously modified chitosan hydrogel TG-CCS loaded with'localized high-response 'lactobacillus salivarius DY802 is constructed. The synthesis process of the material is synergistic and time-saving, has excellent biological safety and tumor inhibition experiment performance, and can comprehensively repair four barriers of the intestinal tract: ROS is removed, and tight junction protein is protected to repair the physical barrier; forming a hydrogel protective layer to reinforce the chemical barrier; the flora diversity is improved, and the prebiotic effect is exerted to repair the biological barrier; the sphingomyelin metabolism and ceramide abundance are regulated and controlled, the abnormal infiltration of NK cells is inhibited, and the immune barrier is remodeled. Therefore, the probiotic hydrogel material disclosed by the invention is clear in treatment mechanism and remarkable in clinical transformation potential and application value.

This invention relates to the field of catalyst preparation technology, and more specifically, to the preparation and application of a metal catalyst for the production of the rubber antioxidant 6PPD. Copper nitrate and zinc nitrate compounds are dissolved in deionized water, and silicate esters are added and mixed in a water bath. The pH is adjusted, the mixture is aged, filtered, and washed with water to obtain the catalyst precursor. Copper halide is added and stirred until homogeneous, then dried in an oven, ground, and calcined to obtain the metal catalyst for the production of the rubber antioxidant 6PPD. This invention's metal catalyst maintains high activity and selectivity while also being low in cost, exhibiting excellent stability and environmental friendliness. The preparation process is simple, reproducible, and suitable for large-scale production, providing a novel catalytic solution with high industrial application value for the efficient and green synthesis of p-phenylenediamine antioxidants.

This utility model relates to a durable and collapse-resistant silicone PU (SPU) sports court, comprising, from bottom to top: a compacted concrete layer, a first compression-resistant layer, a first buffer layer, a second compression-resistant layer, a second buffer layer, and a silicone PU layer; the first compression-resistant layer is formed of crack-resistant mortar; the second compression-resistant layer is formed by an array of several crack-resistant mortar blocks arranged at intervals and filled with shock-absorbing buffer blocks; both the first and second buffer layers are made of rubber or plastic. The silicone PU sports court provided by this utility model has better compression resistance, resistance to local collapse, and crack resistance, while also exhibiting good shock absorption and rebound performance.

This invention provides a fully premixed PCR kit and its Taq DNA polymerase variant. The kit includes primers for PCR amplification, a PCR amplification buffer, and a Taq DNA polymerase mutant. The Taq DNA polymerase mutant is obtained by replacing the amino acid sequence of the 5'–3' exonuclease domain of wild-type Taq DNA polymerase with that of SEQ ID NO.2. The Taq DNA polymerase variant of this invention integrates the endonuclease 1 domain of *Thermococcus barbata*, endowing Tb-Taq with selective 5'→3' exonuclease activity. It has no cleavage activity at the 5' end of blunt-ended double-stranded DNA, while maintaining its hydrolytic ability to TaqMan probes during PCR amplification. This effectively solves the problem of probe cleavage and degradation during long-term storage of fully premixed kits, thereby avoiding phenomena such as increased fluorescence background, decreased fluorescence intensity of the amplification curve, Ct value lag, and significant decrease in detection sensitivity.

The application belongs to the technical field of high-speed object velocity measurement in a closed cavity. The application provides a high-speed object velocity estimation method in a closed cavity based on magnetic characteristic pulse width time difference. In the method, a single fluxgate sensor is arranged on a movement path of a measured target, a magnetic field disturbance signal collected by the fluxgate sensor is combined with signal preprocessing and disturbance mutation segment pulse width detection, a start time and an end time of the magnetic field disturbance signal are extracted, a time width of the target passing through a magnetic flux gate induction area is calculated, and a movement velocity of the target is estimated. The method is completely based on data analysis of the magnetic field disturbance signal collected by the fluxgate sensor, and does not need to contact the measured target or change the structure of the target or add a sensor device during measurement. Moreover, the method does not need to establish a magnetic field mathematical model of the measured target in advance, does not rely on prior knowledge such as magnetic permeability and magnetic moment distribution of the target, and only needs to know a physical width of the target in a disturbance direction.

This invention discloses a ferrophosphate-based high-entropy glass-ceramic containing a high-entropy monazite phase and its preparation method, used for simultaneously solidifying multiple (minor)actinide nuclides in high-level radioactive waste (HLW). This invention uses five or more simulated (minor) actinide nuclides in equal amounts. Ln ( Ln =La, Ce, Nd, Sm, Eu, Gd, Dy, Ho, Er) simultaneously dissolve into the ceramic crystalline phase, forming a high-entropy monazite phase ( Ln 1 / n )PO4(5≤ n ≤7). The chemical formula of the solidified body is (100- x ()( y Fe2O3-(90- y (P₂O₅-10B₂O₃)-0.5 x P2O5−( x / 2 n ()( Ln 2O3)(mol%), x =0~30mol% y =15~35mol%. The raw materials are mixed according to the formula and then subjected to a one-step melt heat treatment method (melting, nucleation, crystallization) to obtain a solidified iron phosphate-based high-entropy glass-ceramic.

The application belongs to the technical field of water treatment, and provides an electro-catalytic periodate synergistic wastewater treatment method and device. The wastewater treatment method comprises the following steps: adding organic contaminant-containing wastewater to be treated into a wastewater treatment device provided with an anode plate and a cathode plate, adding periodate into the wastewater treatment device, starting a direct current power source connected with the anode plate and the cathode plate to perform wastewater treatment, and discharging the treated wastewater; the material of the anode plate is ruthenium oxide-iridium oxide, and the material of the cathode plate is carbon felt; in the wastewater treatment process, the organic contaminant is oxidized and degraded by direct electron transfer on the anode plate, and electro-catalytic oxidation is synergized with periodate to produce active species including OH · , 1 O2,O2 ·‑ ,H2O2,IO3 · and H2O2, IO3- and H2O2, which oxidize and degrade the organic contaminant in the wastewater, and the cathode plate removes part of the contaminant in the wastewater through adsorption. The application can realize efficient and low-cost treatment of wastewater containing refractory organic matter.

The invention relates to a double-sided magnetic power bank with a built-in intelligent temperature control system, and the power bank comprises a bottom plate and a cover plate which are combined to form a housing with a battery compartment inside. The battery is mounted in the battery compartment; the PCB is installed on the inner wall of the cover plate and located on the front side of the battery; the distributed temperature sensing network is composed of a plurality of thermistors arranged at a plurality of key thermal nodes in the power bank and is used for collecting temperature signals; the main control MCU is mounted on the PCB and is coupled to the distributed temperature sensing network; wherein a first magnetic attraction module is arranged in the bottom plate, and a second magnetic attraction module is arranged in the cover plate, so that the double-sided magnetic attraction function of the power bank is realized. The magnetic attraction module and the wireless charging coil are integrated on the two faces of the bottom plate and the cover plate, simultaneous wireless charging of the two high-power devices is achieved, the charging efficiency and the flexibility of use scenes are greatly improved, and the real requirements of multi-device users are met.

The invention discloses an alfalfa salt-tolerant gene MsKUP1 and an application of the alfalfa salt-tolerant gene MsKUP1. On the basis of correlation analysis of phenotypes and genomes, a gene MsKUP1 responding to salt stress is screened from alfalfa, the nucleotide sequence of the MsKUP1 is as shown in SEQ ID No.1, and the amino acid sequence of the MsKUP1 is as shown in SEQ ID No.2. The MsKUP1 overexpressed alfalfa hairy roots are obtained by constructing a vector and a strain of the overexpressed MsKUP1, infecting alfalfa leaves and performing induced differentiation and screening, so that the effect of the MsKUP1 on the salt tolerance of the alfalfa is further verified, and compared with a wild type, the MsKUP1 overexpressed alfalfa hairy roots grow longer under the salt stress, the tolerance to the salt stress is higher than that of a wild plant, and the MsKUP1 overexpressed alfalfa hairy roots have the advantages that the MsKUP1 overexpressed alfalfa hairy roots are better than those of the wild plant, and the MsKUP1 overexpressed alfalfa hairy roots are better than those of the wild plant. The overexpression of the MsKUP1 gene can enhance the salt tolerance of medicago sativa, and theoretical and practical support is provided for cultivating and vigorously popularizing salt-tolerant medicago sativa varieties.

This invention discloses a lens design method based on deep learning, belonging to the fields of optical design and deep learning. The method includes: constructing a dual-channel neural network; adjusting task indicators in real time according to the current state using a dynamic task generator; inputting the task indicators into the dual-channel neural network for joint training; calculating aberration sensitivity based on Zernike aberration coefficients from real-time ray tracing verification feedback; and outputting a lens design result that meets both optical performance and manufacturing feasibility requirements when the closed-loop iteration formed by dual-channel joint training, real-time ray tracing verification, and dynamic optimization strategy adjustment satisfies a preset convergence condition. This invention uses a dynamic task generator to adjust the optimization path in real time, physically constrains channels to block unmanufacturable structures, and prioritizes suppressing dominant aberrations through aberration sensitivity feedback; it embeds lightweight ray tracing into the training loop, provides real-time feedback of Zernike aberration coefficients, and uses manufacturing feasibility scoring to drive design iteration.

The application discloses a preparation method of a rare earth cerium-based coupling Zr3SO9 superacid nanometer catalyst and application of the catalyst in flue gas denitration. The method comprises the following steps: mixing a certain amount of ZrOCl2 with H2SO4, and then performing hydrothermal reaction at 240 DEG C for 72 hours to obtain Zr3SO9 superacid crystal material. After Zr3SO9 and Ce(NO3)3.6H2O are mixed and ground, the mixture is placed in a muffle furnace to be calcined to obtain CeO2 / Zr3SO9. The preparation method has the advantages that the prepared catalyst has a wide active temperature window, excellent sulfur resistance and water resistance, the required raw material resources are rich, the preparation process is simple and environment-friendly, and the catalyst has a potential industrial application prospect.

The present application relates to a kind of reproducible aflatoxin B1 immunoadsorbent and affinity column for purifying.The immunoadsorbent includes solid phase carrier and the aflatoxin B1 nanobody coupled on the solid phase carrier, the aflatoxin B1 nanobody is the aflatoxin B1 nanobody of amino acid sequence as shown in SEQ ID NO:1, or it is as shown in SEQ ID NO:3 Self-assembly multivalent aflatoxin B1 fusion nanobody NbAF-EGFP-H6.The aflatoxin B1 nanobody in the immunoadsorbent of the present application is high in sensitivity, high in specificity, can be used for the specific adsorption of aflatoxin B1, is used as reproducible purification aflatoxin B1 immune affinity column after packing column, for the high performance liquid chromatography-mass spectrometry detection of aflatoxin B1.The preparation step of the immune affinity column of the present application is simple, low in cost, stable in performance, strong in tolerance, high in specificity, good in reproducibility.Provide the possibility for establishing an environmentally friendly, economic, sensitive, fast detection method.

The application discloses a device and method for realizing simultaneous nitrogen removal of urban sewage and photovoltaic cell wastewater based on a reinforced autotrophic / heterotrophic coupled granular sludge process. The device mainly comprises a horizontal flow defluorination reaction sedimentation tank, an intelligent adjusting tank, an aerobic deep oxidation system and a reinforced autotrophic / heterotrophic coupled granular system. The method realizes efficient simultaneous nitrogen removal of urban sewage and photovoltaic cell wastewater through a double-flow inlet strategy: the residual micro-particles and calcium ions after defluorination pretreatment are used as crystal nuclei to promote the granulation of subsequent sludge; the impact resistance of the coupled granular sludge is enhanced through a biofilm carrier; and the intelligent adjusting tank is used to realize precise water distribution, so that the reinforced autotrophic / heterotrophic coupled granular system can stably and efficiently remove nitrogen. The application not only realizes simultaneous deep treatment of urban sewage and photovoltaic cell wastewater, but also strengthens the structural stability of the granular sludge by using the calcium components in the wastewater, solves the problem of difficulty in forming the granular sludge and improves the nitrogen removal performance.

The invention belongs to the technical field of biology, and particularly relates to a Klebsiella pneumoniae bacteriophage PKS and application thereof. The invention provides a Klebsiella pneumoniae bacteriophage PKS, and the preservation number of the Klebsiella pneumoniae bacteriophage PKS is CGMCC No. 46660. The invention provides a brachyphage, the head of which is of a polyhedral structure, the diameter is 55 + / -1nm, the tail is short, thick and non-shrinkable, and the length is 10 + / -1nm; the incubation period of the klebsiella pneumoniae bacteriophage PKS is 30 min, the lysis period is 60 min, the lysis amount is 747 PFU / cell, the virus titer can reach 1013, the activity stable pH is 4-11, and the activity stable temperature is-20 DEG C to 65 DEG C. The Klebsiella pneumoniae bacteriophage PKS is wide in host range, high in temperature and pH tolerance, capable of adapting to various environments and capable of effectively and specifically solving the problem that Klebsiella pneumoniae has drug resistance to various antibiotics.

The utility model discloses a high -end inverter high -current end piece formula capacitor, including by the core, and the capacitor shell that cladding setting in the capacitor main part of core outside is constituted, the both ends of core are connected with the connecting terminal through the electrode sheet, and the connecting terminal is including the terminal main part, and is set up in the head of terminal main part, and with the plug connector of electrode sheet connection, the tail of plug connector is equipped with the card hole. The utility model solves the problem of insufficient of capacitor in prior art to the high current resistance, and the high heat release, the fast temperature rise and the short service life.

The invention discloses a strain of Bacillus sterilis GDB-1 and an application of the strain of Bacillus sterilis GDB-1. According to the invention, a strain of Bacillus sterilis GDB-1 is separated from soil, and is preserved in Guangdong Microbial Culture Collection Center on July 9, 2025, and the preservation number is GDMCC NO.66655. According to the invention, the strain of Bacillus sterilis GDB-1 is preserved in Guangdong Microbial Culture Collection Center on July 9, 2025. Experiments prove that the strain can inhibit the growth of various different phytophthora, has good tolerance to common pesticides and heavy metal ions, and has a good application prospect in the field of biological control; in addition, the strain can promote the growth of plants, and can also be used for preparing a plant growth promoting preparation.

The invention discloses a segmented cooling system and method for evaporating and crystallizing fly ash water washing liquid to obtain sylvite, and belongs to the field of fly ash water washing liquid treatment. The system comprises a pretreatment and evaporation sodium separation unit, a vacuum flash evaporation cooling unit, an indirect heat exchange cooling crystallization unit and a separation drying unit which are in fluid communication in sequence, the vacuum flash evaporation cooling unit adopts a structure without a heat exchange wall surface and is matched with a condensation system capable of recycling heat energy; and the indirect heat exchange cooling crystallization unit is matched with a forced circulation and external heat exchange structure. According to the method, a segmented process of vacuum flash evaporation precooling and indirect heat exchange deep cooling is adopted, firstly, the potassium-rich mother liquor at the temperature of 100-110 DEG C is cooled to 55-65 DEG C through vacuum flash evaporation, and then is cooled to 20-30 DEG C through indirect heat exchange, so that potassium chloride is fully crystallized. According to the method, the energy consumption of a vacuum system can be reduced by 30-50%, scaling of a high-temperature section is eradicated fundamentally, the product purity is guaranteed to reach 90% or above through a flash evaporation precipitation-cooling redissolution self-purification mechanism, and efficient, low-consumption and long-period stable recovery of potassium chloride in the fly ash water washing liquid is achieved.

The present application relates to the technical field of paint, in particular to a kind of bio-based polyurethane anticorrosive paint, its preparation method and application;The present application is to improve the waterproof performance and anticorrosive performance of bio-based polyurethane anticorrosive paint, and fluorosilicon modified three-arm isocyanate is synthesized and added as a modified material for modifying bio-based polyurethane;The fluorine element and silicon element introduced in this process have very strong hydrophobic property, and C-F and Si-O bond are high, have stronger resistance to external ultraviolet and saline-alkali erosion, and the crosslinking network formed by three-arm symmetrical structure is more complex, crosslinking density is higher and shielding performance is better, so as to realize the enhancement of coating protection performance on multiple levels.

The present application relates to a kind of aerobic granular sludge and its fast culture method, including (1) in reactor inoculation aerobic activated sludge, using low ammonia nitrogen high COD culture solution is cultivated under low dissolved oxygen condition, obtain functional aerobic sludge;(2) in step (1) system, add culture solution and regulator, and inoculate functional inoculant, cultivate under high-low dissolved oxygen alternation condition, obtain primary granular sludge;(3) primary granular sludge is cultivated using culture solution containing chitosan, obtain final aerobic granular sludge.The present application can realize the rapid formation of granular sludge, the adaptability of the obtained granular sludge is strong, functional flora is stable, and long-period operation effect is good, and can be used for the biochemical treatment process of various toxic or refractory organic pollutants, nitrogen and phosphorus containing industrial wastewater.

This invention discloses a L-carnitine refining system, belonging to the field of L-carnitine refining. It includes an anode plate, a first cation exchange membrane, at least one repeating unit, a third cation exchange membrane, and a cathode plate arranged sequentially from left to right. The repeating unit includes a first anion exchange membrane, a second anion exchange membrane, and a second cation exchange membrane arranged sequentially from left to right. A first electrode chamber is formed between the anode plate and the first cation exchange membrane; a first recovery chamber is formed between the first cation exchange membrane and the first anion exchange membrane; a feed chamber is formed between the first anion exchange membrane and the second anion exchange membrane; a replenishment chamber is formed between the second anion exchange membrane and the second cation exchange membrane; a second recovery chamber is formed between the second cation exchange membrane and the third cation exchange membrane; and a second electrode chamber is formed between the third cation exchange membrane and the cathode plate. This invention replaces the bipolar membrane with a conventional anion / cation exchange membrane combination, reducing the loss of active ingredients to improve yield, and has low energy consumption.

This invention belongs to the field of microbial technology and discloses a strain of Acinetobacter baumannii and its applications. The invention provides an Acinetobacter baumannii strain deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M 20261099. This strain exhibits excellent degradation performance in both soil and water remediation, filling a gap in the application of existing BPA-degrading bacteria in industrial sites.

The present application belongs to the technical field of welding manufacturing of large steel structure, and particularly relates to a multi-layer and multi-pass full penetration welding process for a right-angle structure. The welding process comprises the following steps: cutting a double-sided asymmetric groove on a rib plate, one side of which is a large groove and the other side is a small groove, and polishing the groove; fixing the rib plate and a spoke plate on the small groove side by using a process triangular plate; welding the rib plate and an outer ring as well as the spoke plate, the rib plate and the spoke plate being welded as horizontal welding, and the rib plate and the outer ring being welded as vertical welding, and removing the process triangular plate; and performing non-destructive testing and annealing heat treatment on the weld. The welding process reduces labor intensity, improves the working environment, and ensures the stability and consistency of the welding quality.