1218results about How to "Strong tolerance" patented technology

The invention relates to a technology for high-density fermentation of Lactobacillus casei (Lactobacillus casei Zhang) with prebiotic efficacy, which comprises optimal controls on a proliferation culture medium and the condition of a fermentation process, and a technology for preparing high-vitality freeze-dried powder of the strain. With the technology, the freeze-dried powder of the Lactobacillus casei with more than 10<11> cfu per milliliter of viable bacteria can be obtained, and the freeze-dried powder can be directly applied to the preparation and the application of a probiotic hametsz and a probiotic preparation of the strain.

The invention relates to an ecological slope protection type sewage land infiltration system which comprises a grid pond (2), an anaerobic hydrolyzing pond (4) and a stepped land infiltration unit which are sequentially arranged along the gradient of a sloping field from top to bottom, wherein the grid pond (2) is communicated with a ditch (1); the stepped land infiltration unit comprises multilevel slope protection infiltration beds (5); filter materials (7) are filled in the slope protection infiltration beds (5); hygrophyte communities (9) are cultivated on the slope protection infiltration beds (5); a modal conversion assembly (6) used for controlling infiltration speed, waterpower retention time, a filtering layer internal environment and an effluent water mode is arranged between adjacent slope protection infiltration beds (5). The ecological slope protection type sewage land infiltration system disclosed by the invention tolerates the rural sewage quality water flow impact, is difficult to block due to high reaeration capacity, intensifies the denitrification and dephosphorization effect, is green, energy-saving, economic and practicable and has excellent water conservancy and ecology value.

The invention discloses a distributed parameter measurement impedance-based relay protection method for a single-phase grounding fault of a power transmission line. The method comprises the following steps that: fault-phase distributed parameter measurement impedance Zphi,ce is directly calculated by utilizing fault-phase voltage, fault-phase current, fault-phase negative-sequence current and zero-sequence current of a protector mounting position and parameters of the power transmission line; and if the amplitude of the fault-phase distributed parameter measurement impedance is lower than a set impedance value, and a phase angle of the fault-phase distributed parameter measurement impedance is within the range of 45 to 135 degrees, a protector transmits a tripping signal, otherwise the protector does not act.

The invention relates to a robust state estimation method in an electric power system based on exponential type objective function, belonging to the field of electric power system dispatching automation. The method comprises the following steps: building a new state estimation mathematical model of the exponential type objective function according to the electric network model of the actual electric network and real-time measuring data; estimating the busbar voltage and phase angle of the electric network, thus calculating the active power and reactive power of the circuit, a transformer, a generator, loads and other devices. The invention can automatically realize that the impact of measurement with big errors on the objective function tends to zero, while the impact of correct measurement on the objective function tends to one. Therefore, the state estimation algorithm proposed in the invention can process in the absence of bad data identification, thus the invention has convenient realization and high computing efficiency; furthermore, if the system is absent of bad data, the estimated performance in the invention is similar to the classical weighted least squares method.

The invention discloses a lactobacillus plantarum and a preparation method thereof for high-density culture and freeze-drying bacteria powder, and has the technical scheme that the lactobacillus plantarum (Lactobacillus plantarum P8) is probiotics separated from the traditional fermentation milk product (yoghurt) of the Urat Middle Banner of Inner Mongolia Bayan Nur. The probiotics has the characteristics of artificial gastric juice and artificial digestive juice tolerance, cholate tolerance, intestinal agglutination action and common pathogenic entero bacteria inhibition. The bacterial strain of the bacteria is collected in the China general microbiological culture collection center with the collection number of CGMCC (China general microbiological culture collection center) No. 5468 on 18th, Nov., 2011.

The invention discloses a preparation method of remediation flora for Cr (VI) contaminated site from sludge. The method uses sludge as the main source of bacteria strain and high-concentration organic wastewater as nutrient solution, which are supplemented by appropriate additives and subjected to special biochemical culture, so as to prepare corresponding remediation flora for quick remediation of the (Cr VI) contaminated site. The remediation flora can quickly and effectively remediate Cr (VI)-containing oil on the site.

The invention belongs to the technical field of veterinary microbial additive preparation, and specifically relates to a strain of separated and screened Enterococcus faecium providing significant bacterial inhibition effects for common enteropathogenic bacteria such as staphylococcus aureus, escherichia coli and salmonella in breeding animals, and an application thereof. The probiotic Enterococcus faecium of the present invention is characterized in that: the strain is Enterococcus faecium HDRsEf1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CCTCC NO:M2011031. The probiotic Enterococcus faecium of the present invention has characteristics of fast growth, high acid production capacity, strong stress resistance, safety, disease resistance and growth promotion, and can be used as the microbial feed additive for livestock and poultry feeds.

The invention discloses a mussel mucoprotein gel for repairing and reliving itching. The mussel mucoprotein gel is prepared from the following components in percentage by weight: 0.15%-0.2% of mussel mucoprotein, 0.5%-2% of a macromolecule host material, 3%-10% of a humectant, 1%-5% of a surfactant, 0.1%-0.5% of a preservative, 1%-2% of a protein protectant, 0.5%-3% of a stabilizer, 0.5%-1% of a bacteriostatic agent, 0.8%-5.2% of a pH modifier and the balance of purified water. The mussel mucoprotein gel has good biological adhesion, is rapidly and effectively adhered to a wound surface, is capable of rapidly reliving itching and easing pain, and has the advantages of being low in immunogenicity, good in biocompatibility with a human body, convenient to use, free of thrill to skin, safe, nontoxic and free of bad reaction.

The invention discloses a method for repairing soils polluted by heavy metal Cd, characterized in that a plant used for repairing the metal Cd is Cd-tolerant tobacco K326, a used soil chelation inducer is a hydrochloric acid solution of chitosan, microbes used for activating the heavy metal Cd in the soils are bacterial strain obtained by gathering, screening and separation in a laboratory, the three substances are combined to repair the soils polluted by the heavy metal Cd. The method comprises the following steps: cultivating a K326 seedling to the 3-leaf one-heart stage, then transplanting; before transplanting, adding chitosan; before transplanting, carrying out mass fermental cultivation of separated, screened and purified bacterial strain, adding the active bacterial strain of the heavy metal Cd at the logarithmic phase in the soils, and stirring for the subsequent use; and after transplanting the tobacco plant, regularly carrying out management. According to the invention, a plant-microorganism united repair method is adopted, and a certain amount of microbes of the active heavy metal are added in the soils to effect together, thus the repair efficiency is raised, and the efficiency of repairing the soils polluted by the heavy metal Cd is raised.

The invention discloses a method for hydrolyzing lignocellulose. Lignocellulose is pulverized into particles, and the particles are mixed with water to form even seriflux; the seriflux is preheated to60-150 DEG C by using ultrasonic waves or microwaves; the preheated even seriflux is quickly heated to 120-300 DEG C, solid alkali is added and used as a catalyst, and stirring and catalytic reactionare simultaneously carried out for 15-300 minutes. The use of the ultrasonic waves or microwaves radiation in synergism with solid alkali for catalyzing and hydrolyzing the lignocellulose can greatlyreduce the reaction time as well as energy consumption. The solid alkali catalyst can be repeatedly used, is easily separated from an aqueous solution, and has the advantages of high mechanical strength, high durability, certain selectivity, relative singularity of catalytic hydrolyzate, high hydrolyzing efficiency and good market application prospect.

The invention discloses a method for hydrolyzing lignocellulose. Lignocellulose is pulverized into particles, and the particles are mixed with water to form even seriflux; the seriflux is preheated to60-150 DEG C by using ultrasonic waves or microwaves; the preheated even seriflux is quickly heated to 120-300 DEG C, solid acid is added and used as a catalyst, and stirring and catalytic reaction are simultaneously carried out for 15-300 minutes. The use of the ultrasonic waves or microwaves radiation in synergism with solid alkali for catalyzing and hydrolyzing the lignocellulose can greatly reduce the reaction time as well as energy consumption. The solid acid catalyst can be repeatedly used, is easily separated, and has the advantages of high mechanical strength, high durability, certainselectivity, relative singularity of catalytic hydrolyzate, high hydrolyzing efficiency and good market application prospect.

The disclosure relates to a distributed optical fibre sensor having an optical switch arranged to selectively and simultaneously couple each of a plurality of interrogators to each of a plurality of sensing optical fibres.

The invention discloses a Trichoderma viride F4 strain, and applications thereof. The Trichoderma viride F4 strain is stored at China General Microbiological Culture Collection Center on 10th November 2008, and preservation number is CGMCC No.2736. When the bacterial strain is used for producing cellulase taking pretreated lignocellulose as a carbon source and an inducer, the bacterial strain is capable of tolerating a plurality of inhibitors brought by pretreated lignocellulose, and producing enzyme with high efficiency; the secreted cellulase is comprehensive in composition, enzyme activity is high, and enzymolysis adaptability on pretreated lignocellulose substrate is high.

The invention discloses a pseudomonas putida (Pseudomonas putida) WXZ-4 CGMCC No.1798 strain with aerobic denitrifying property and a method to denitrify the waste water; which is characterized by the following: possessing strong tolerance degree for dissolved oxygen; deacidizing nitrate and nitrite with distinctive speed under the condition of 50-98% saturated dissolved oxygen; seeding to active sludge; proceeding enriched culture; realizing the aerobic anti-nitration.

The invention discloses a method for realizing shortcut nitration of high ammonia nitrogen wastewater. The method comprises the following steps of: culturing nitrosation dominant flora; and performing ammonia sewage treatment by using the flora as an inoculum, namely adding the cultured nitrosation dominant flora into an aeration reactor to treat the ammonia wastewater, wherein the adding amount of the cultured nitrosation dominant flora accords with a condition that: sludge concentration is 800-2,000mg / L after the cultured nitrosation dominant flora is added; and the treatment conditions arethat: the temperature is 18-40DEG C, the dissolved oxygen is 0.1-3mg / L, and the pH is 7- 9. The unique nitrosation dominant flora culture method is adopted, stable and high-efficiency ammonia nitrogen wastewater treatment effect can be achieved, and the problem of instability of the shortcut nitration in actual application is solved.

The invention discloses Lactobacillus plantarum ZN-3 and application thereof. The Lactobacillus plantarum ZN-3 provided herein is collected in CCTCC (China Center for Type Culture Collection) under CCTCC NO: M 2017286. The Lactobacillus plantarum ZN-3 provided herein features acid tolerance and cholate tolerance, can be implanted to intestines via the stomach to inhibit zoonotic pathogens, including gram-negative bacteria that includes Escherichia coli, salmonella, Pasteurella multocida, Erysipelothrix rhuriopathiae, Haemophilus parasuis and porcine infectious pleuropneumonia, as well as gram-positive bacteria that include Streptococcus suis, Staphylococcus aureus, and Staphylococcus epidermidis. Compared with antibiotics, the Lactobacillus plantarum ZN-3 has good antibacterial effect for pathogenic bacteria having high drug tolerance.

The invention discloses a method for culturing a nitrosation dominant microflora, which comprises the following three culture stages: a first stage of enriching mixed microflora of nitrosobacteria and nitrobacteria; a second stage of elutriating nitrobacteria by using a high-temperature culture and normal-temperature culture alternating process, gradually improving the dominate position of the nitrosobacteria, and transferring into a third stage of culture when a nitrosation rate is more than 50 percent; and a third stage of changing the dissolved oxygen and pH condition to perform further elutriation of nitrobacteria and the stable domestication of nitrosobacteria till the nitrosation rate is stabilized over 65 percent. Thus, a culture period is finished, a microflora in which the nitrosobacteria take a dominate position is obtained, and the microflora is stored for later used. The method solves the problems of instability and the like in the actual use of short-process nitration, the start time of a nitration process can be shortened, a nitration reaction process can be changed quickly, the application range of short-process nitration can be expanded, and a guarantee is providedfor the actual use of the short-process nitration process in practical projects.

The invention discloses a method for preparing a probiotic brown lactobacillus beverage product with large amount of bacteria, which comprises the steps of Maillard reaction, probiotic fermentation and post treatment, wherein the probiotic fermentation step comprises: hydrating and sterilizing defatted milk powder or defatted fresh milk, reacting the defatted milk powder or the defatted fresh milk for 2.5 hours at the temperature of between 95 and 98 DEG C till the milky yellow becomes brown at the end point of the reaction, cooling the reaction product to between 37 and 38 DEG C, inoculating the reaction product to a Lactobacillus casei strain, statically fermenting the strain for 48 to 72 hours at the temperature of between 37 and 38 DEG C till the pH reaches 3.8 to 4.0, and cooling the fermentation product to between 2 and 6 DEG C; and the post treatment steps comprises: preparing base solution by using sugar, high fructose corn syrup, pectin and purified water, wherein the weight ratio of the base solution to the probiotic fermentation solution is 1 to 3; and the pH is adjusted to between 3.5 and 3.6; homogenizing the base solution, cooling the base solution to between 2 and 6 DEG C, canning products after passing detection, maturing the products for 4 to 8 hours at the temperature of between 2 and 6 DEG C, and delivery the products from a storage. The number of the living bacteria in the shelf life of the product can reach 1*108 to 1*1,010cfu / ml; and the product has zero fat and super clear mouthfeel, has no viscosity, has high probiotic detection rate, and can effectively keep or recover the intestinal microbial balance.

The invention discloses a vehicle model dynamic identification method used in an intelligent transportation system. The vehicle model dynamic identification method is characterized by comprising the following steps: a step a is a learning and training step of a vehicle model of a moving vehicle, wherein HOG and GIST characteristics are extracted after a resolution ratio is normalized, then classification learning is conducted through a support vector machine, and a first classifier identification model and a second classifier identification model are obtained correspondingly; a step b is an identification step of the vehicle model of the moving vehicle, wherein the moving vehicle is extracted according to a corresponding moving object partitioning algorithm, the extracted HOG and GIST characteristics are input to a first identification model and a second identification model respectively for primary prediction after the resolution ratio is normalized, and a first primary result and a second primary result are obtained respectively; a step c is a primary result fusion step, wherein the first primary result and the second primary result are input and fused through a D-S evidence theory fusion rule, a maximum probability value is obtained, and the identification of the vehicle model of the moving vehicle is finished. The vehicle model dynamic identification method used in the intelligent transportation system is simple in structure, low in complexity, high in algorithm efficiency, and particularly suitable for being used in the intelligent transportation system.

The invention relates to a composite mineral-leaching bacteria colony and use of the same in biological metallurgy. The composite mineral-leaching bacteria colony has a preserving number of CGMCC No.2395 in the Common Micro-organism Center of China General Microbiological Culture Collection Center. The composite mineral-leaching bacteria colony has strong oxidative activity within a temperature range from 30 to 52 DEG C as well as a working pH value of between 0.8 and 2.25 and an arsenic ion tolerance of 0 to 20 g / L. The composite mineral-leaching bacteria colony is used for extracting and recovering gold or copper, and greatly expands the use range of biological metallurgy in gold mines. Meanwhile, due to the wide temperature adaptation range, the composite mineral-leaching bacteria colony improves the oxidation speed and reduces production cost. Therefore, the composite mineral-leaching bacteria colony has a significant application prospect in biological metallurgy industry.

The invention discloses a dynamic avoidance method for multiple robots in collaborative operation. The method comprises the steps: carrying out the path pre-planning of all tasks in a task pool according to the priorities, and reducing the conflict intervention between robots from a planning layer; monitoring the current operation states of all the robots, dynamically pre-judging the safety distance, generating different types of stop points, coordinating the operation of the robots, and solving the technical problems of collision, delay, interlocking and the like during the cooperative operation of the robots. Overall scheduling is carried out to optimize resource distribution, traffic control and coordinated avoidance are achieved, local peer-to-peer communication solves the overall operation problem after the robot individual is abnormal, and therefore the robustness of the whole scheduling system is guaranteed. The method is suitable for multi-robot scheduling avoidance scenes suchas factory automation and storage commodity transfer.

The invention discloses a method for culturing granular sludge by shortcut denitrification. The method comprises the following steps of putting inoculated sludge into an aerobic reactor, and performing enrichment culture on denitrifying sludge by using high ammonia-nitrogen low COD wastewater; then, performing secondary enrichment screening by wastewater simultaneously containing nitrate nitrogen, nitrite and COD in a facultative anaeration reactor, and performing third enrichment screening by wastewater only containing nitrite and COD; and finally acclimating by wastewater simultaneously containing ammonia-nitrogen, nitrite and COD to obtain the denitrified granular sludge with high removal rate on the nitrite when various forms of nitrogen coexist commonly. By the method, the denitrified granular sludge which can survive in a complicated environment and is capable of effectively treating wastewater nitrous nitrogen under proper condition can be obtained, and the shortcut nitrification-denitrification process can be truly applied to actual engineering conveniently.

The invention relates to a microcapsule embedding preparation method of a Lactobacillus casei Zhang microcapsule Zhang CGMCC No.1697, comprising the following steps: activating Lactobacillus casei strain, preparing culture medium, preparing strain fermentation liquor, preparing microcapsule wall material, preparing microcapsule embedding solution and microcapsule.Compared with Lactobacillus casei which is not embedded, the Lactobacillus casei microcapsule prepared by the method of the invention, after being processed in artificial digestive juice, can have viable count increased by one magnitude order.After being preserved for 30 days at 37 DEG C, the Lactobacillus casei microcapsule can have viable count increased by more than one magnitude order.Therefore, the Lactobacillus casei microcapsule prepared by the method of the invention can greatly improve efficacy of probiotics in the process of processing, storage and transportation.

The invention discloses a salinivibrio strain which can carry out biological denitrification under high-saline conditions, and an application thereof. The salinivibrio strain provided by the invention is collected in China General Microbiological Culture Collection Center (CGMCC) on 29th March, 2012 with a collection number of CGMCC No.5946. With a salinity (calculated according to NaCl) below 3-10%, the strain is placed in nitrogen-containing wastewater with dissolved oxygen of 2-6mg / L, such that biological denitrification can be carried out. The strain has high tolerance to high salinity, and grows well in a high-saline condition. The strain has both heterotrophic nitrification and aerobic denitrification capacities. Under a high-saline aerobic condition, the strain can carry out synchronous nitrification and denitrification, such that total nitrogen in sewage can be completely removed with high efficiency. Therefore, a major problem of biological denitrification under high-saline conditions is effectively solved, and a good application prospect is provided.

The invention relates to a high-density culture method of bifidobacterium lactis V9 and a preparation method of a freeze-dried powder of the bifidobacterium lactis V9, and belongs to the field of probiotics fermentation. The bifidobacterium lactis V9 (Bifidobacterium animalis subsp.lactis V9) is acid-resistant and cholate-resistant probiotics which are separated from the intestinal tract of a healthy Mongolian child, and the collection number of the bifidobacterium lactis V9 is CGMCC No.5470. High-density fermentation of the bacteria of the bifidobacterium lactis V9 is realized under the control of an optimized culture medium and optimized fermentation conditions; the viable count in a fermentation liquor can be more than 2.0*10<10>cfu / ml, and the viable count in the freeze-dried powder of the bifidobacterium lactis prepared by using the fermentation liquor can be more than 6.0*10<11>cfu / g, and therefore, the freeze-dried powder of the bifidobacterium lactis can be applied to a probiotic leavening agent or a probiotic preparation.

The invention relates to a heterotrophic nitrification-aerobic denitrification compound bacterial agent for low-temperature high ammonia-nitrogen removal and application. The compound bacterial agentis formed by compounding of 10-20% of cupriavidus SWA1, 5-20% of alcaligenes faecalis, 10-30% of acinetobacter and 20-50% of ochrobacterum TAC-2. The compound bacterial agent has advantages of high ammonia-nitrogen resistance, low-temperature resistance, heavy metal resistance and synchronous nitrification and denitrification; by synergistic effects of four microorganisms, efficient denitrification of high ammonia-nitrogen wastewater can be realized in an aerobic environment under low-temperature conditions.

A process for producing biological diesel oil by fixed enzyme method is carried out by taking sodium alginate, kaoline, glutaraldehyde, gelatin and lactose as co-fixer, fixing the lipase, piling the spherical fixed enzyme in filled bed columnar reactor naturally, alcoholyzing animal and plant oil fatty with biological diesel oil as solvent and producing biological diesel oil. It is cheap, has less consumption and by product and better quality. It can be used for continuous production.

The invention provides a preparation method of N / S-codoped carbon microspheres with high oxygen reduction catalytic activity. The preparation method comprises the following steps: adding a carbon source, a nitrogen source, a sulfur source and an analytically pure surfactant into a stainless steel reaction kettle in sequence under nitrogen protection, stirring, sealing, heating in a crucible furnace, and taking out a mixture when the reaction kettle is cooled naturally to room temperature; washing and filtering the mixture; drying obtained powder in a vacuum drying oven; uniformly mixing an obtained product with analytically pure potassium hydroxide powder, activating in an argon atmosphere; washing with distilled water; and finally, drying in vacuum in the vacuum drying oven. The preparation method has the advantages of simple process, mild reaction condition, high repeatability and low cost; in an alkaline environment, the generated product has excellent oxygen reduction catalytic property, is relatively high in catalytic activity, good in stability and relatively good in tolerance to methyl alcohol and carbon monoxide and has huge application potential on the aspect of fuel cell catalysts.

The invention discloses lactobacillus plantarum and application thereof. A strain of the lactobacillus plantarum is PA01 and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 15660. The lactobacillus plantarum has high safety and strong tolerance, can be colonized on intestines and can grow and reproduce on the intestines; the lactobacillus plantarum isused for adjusting the balance of intestinal flora and expressing a probiotic function; the lactobacillus plantarum can be used for preparing foods or feed additives, regulators for regulating intestinal diseases of animals, animal antibiotic substituting agents, immunoregulators and pathogenic bacterium bacterium-inhibition agents, and also can be used for improving the production performance ofbroiler chickens; the lactobacillus plantarum has a good effect, is safe and has no side effect.