219results about How to "Strong tolerance" patented technology

The invention discloses an antibody for resisting HPV16 type E6 protein and E7 protein, a detection product and application of the antibody and the detection product, and relates to the technical field of monoclonal antibodies. The antibody comprises a heavy chain complementarity determining region in a heavy chain variable region as shown in SEQ ID NO: 1 and a light chain complementarity determining region in a light chain variable region as shown in SEQ ID NO: 2. The antibody can be specifically combined with HPV16 E6 protein and E7 protein, and a detection product for detecting the HPV16 E6 / E7 antigen can be developed based on the antibody provided by the invention. Tests show that a kit developed based on the monoclonal antibody has the advantages of high detection sensitivity, rapidness and strong tolerance on the HPV16 E6 / E7 antigen, can be used for quality control in the production process of the recombinant human HPV16 E6 / E7 antigen, and can efficiently and accurately quantify the antigen content in a vaccine and monitor the quality of the vaccine.

The invention belongs to the field of biological medicine, and relates to an anti-SEZ6 nano antibody or antigen binding fragment and application. The anti-SEZ6 nano antibody or the antigen binding fragment comprises three complementary determining regions, namely, CDR1, CDR2 and CDR3; the sequences of all the regions are respectively shown as SEQ ID NO: 1 to SEQ ID NO: 18. Compared with a conventional antibody, the anti-SEZ6 nano antibody is efficient in expression, simple in purification, small in molecular weight, high in affinity, relatively good in specificity, high in solubility and strong in tolerance, and can be widely applied to the fields of scientific research, diagnosis and treatment.

The invention discloses an antibody for resisting HPV16 and / or HPV18 type E2 protein, an antibody conjugate and application of the antibody conjugate, and relates to the technical field of medical biology. The antibody comprises a heavy chain complementarity determining region in a heavy chain variable region as shown in SEQ ID NO: 1, and a light chain complementarity determining region in a light chain variable region as shown in SEQ ID NO: 2. Tests show that a kit developed based on the monoclonal antibody has the advantages of high detection sensitivity, good specificity, high intermediate precision, high accuracy, consistent linear range of standard curves, fast detection and strong tolerance for the HPV16 and / or HPV18 type E2 antigen, can be used for quality control in the production process of the recombinant human HPV16 and / or HPV18 type E2 antigen, and has wide application prospects. The antigen content in the vaccine can be efficiently and accurately quantified, and the quality of the vaccine can be monitored.

The invention belongs to the field of water ecological restoration, and particularly relates to a riverway water ecological restoration method based on an ecological floating blanket, ecological floating blanket-fish-microorganism is utilized to construct an ecological restoration system to improve a riverway water body, and the ecological floating blanket comprises plants, a carrier, a nylon net, a planting matrix and an inorganic filler; the selected plants are subjected to pollutant degrading bacterium inoculation and tolerance experiment domestication; inorganic filler is hung on the lower part of the ecological floating blanket through a nylon net; the nylon net is subjected to membrane pre-hanging treatment, so that a layer of yellowish-brown biological membrane is formed on the surface of the nylon net; the planting substrate is a combination of multiple of chitosan-potassium alginate coating melamine polyurethane composite foam, biochar, scrap iron and alum sludge ceramsite; the plant provided by the invention is high in tolerance and can efficiently adsorb and degrade pollutants; the planting matrix provided by the invention promotes each other and efficiently adsorbs pollutants; the constructed ecological floating blanket-fish-microorganism ecological planting and breeding technology can promote a receiving river channel to form a food chain system with a healthy water body.

The invention discloses a Klebsiella phage and application thereof, and belongs to the technical field of life science. The Klebsiella bacteriophage is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.46000, the preservation date is July 22, 2024, and the Klebsiella bacteriophage is classified and named as FK9. The bacteriophage provided by the invention is a biological means capable of efficiently killing klebsiella pneumonia, has the characteristics of high efficiency, specificity and small side effect, achieves the effect of preventing and treating pneumonia caused by klebsiella pneumonia on the basis of not destroying in-vivo flora balance, is incomparable to antibiotics and other antibacterial drugs, and has a broad application prospect. And meanwhile, the method contributes to sustainable development of a health medical system.

The invention discloses lactobacillus reuteri with antioxidant, anti-inflammatory and anti-hypoxia capabilities, the name of the lactobacillus reuteri is QM11, the classification name of the lactobacillus reuteri is CGMCC NO.31541, the preservation number of the lactobacillus reuteri is CGMCC NO.31541, the preservation date of the lactobacillus reuteri is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing on August 5, 2024, and the preservation number of the lactobacillus reuteri is CGMCC NO.31541, the preservation number of the lactobacillus reuteri is CGMCC NO.31541, the preservation number of the lactobacillus reuteri is CGMCC NO.31541, and the preservation number of the lactobacillus reuteri is CGMCC NO.31541. The preservation unit is China General Microbiological Culture Collection Center (CGMCC). The lactobacillus reuteri is separated from faeces of healthy yaks in Qinghai province, has relatively strong acid and cholate tolerance, has relatively good antioxidant and anti-inflammatory capacities, and has a relatively strong inhibition effect on pathogenic bacteria such as yak-derived pathogenic escherichia coli, staphylococcus aureus and salmonella typhimurium; the sodium dextran sulfate induced mouse colitis symptom is relieved, and the hypoxia tolerance of the mouse is improved. The lactobacillus reuteri disclosed by the invention can be used for preparing medicines or microecological preparations for resisting oxidation, inflammation and hypoxia, and has a very wide application prospect.

The invention discloses lactobacillus brevis JS231 for high yield of gamma-aminobutyric acid and application of the lactobacillus brevis JS231, and belongs to the technical field of biology, the lactobacillus brevis JS2311 is preserved in Guangdong Microbial Culture Collection Center on August 30, 2024, the preservation address is building 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC 65079. The invention also provides any one of the following applications of the lactobacillus brevis JS231: (1) application in production of gamma-aminobutyric acid; (2) application in preparation of a fermentation product with antioxidant activity; (3) application in inhibiting the activity of pathogenic bacteria; (4) application in degradation of nitrite; and (5) application in development of lactic acid bacteria products rich in gamma-aminobutyric acid. The lactobacillus brevis JS231 provided by the invention provides an original material for the development of lactic acid bacteria resources.

The utility model discloses a denitrification treatment system for wastewater containing high ammonia nitrogen and refractory organics, and belongs to the technical field of wastewater treatment. The system comprises a coarse / fine grid, an adjusting tank, an anaerobic reaction tank, an anaerobic sedimentation tank, a Fenton reaction tank, an iron ammoxidation adjusting tank, an iron ammoxidation reaction tank, an iron ammoxidation sedimentation tank, a liquid alkali sedimentation tank, a sulfur autotrophic adjusting tank and a sulfur autotrophic tank which are sequentially communicated along the wastewater treatment direction, at least part of sludge in the anaerobic sedimentation tank flows back to the anaerobic reaction tank, at least part of sludge in the iron ammonia oxidation sedimentation tank flows back to the iron ammonia oxidation reaction tank, and at least part of sludge in the liquid caustic soda sedimentation tank flows back to the Fenton reaction tank; and residual sludge in the anaerobic sedimentation tank, the iron ammonia oxidation sedimentation tank, the liquid caustic soda sedimentation tank and the sulfur autotrophic tank sequentially enters a sludge concentration tank and sludge dewatering equipment. On the premise of meeting the production and environmental protection requirements, the operation and sludge treatment cost is reduced, and the operation stability of the treatment system is improved.

The invention discloses a fermentation process and a preparation of lactobacillus reuteri L-01, and belongs to the field of microorganism preparation.After artificial domestication, bile salt tolerance of the lactobacillus reuteri L-01 is enhanced, the lactobacillus reuteri L-01 can be cultured in gastrointestinal fluid, and the survival rate of 98% or above can still be kept for a long time; the lactobacillus reuteri L-01 disclosed by the invention can play an excellent probiotic function, is beneficial to improving the balance of intestinal flora, has an inhibiting effect on pathogenic bacteria, and reduces the prevalence rate. Besides, after the components of the culture medium are optimized, the domesticated lactobacillus reuteri L-01 also has a certain anti-oxidation effect and is rich in selenium, so that the use value of the lactobacillus reuteri L-01 is increased. The lactobacillus reuteri L-01 fermentation broth is obtained, after a protective agent is added, the more excellent probiotic effect can be guaranteed, the stability of the lactobacillus reuteri L-01 in processing application and transportation storage is enhanced, and the lactobacillus reuteri L-01 fermentation broth has a certain slow-release effect and has a longer acting effect.

The invention belongs to the technical field of probiotics, and particularly relates to pediococcus acidilactici with acid resistance, bile salt resistance and antibacterial activity and application of the pediococcus acidilactici. The pediococcus acidilactici TR19 has the preservation number of CGMCC NO.31330 and is classified and named as pediococcus acidilactici according to microorganisms. The strain has good acid resistance and bile salt resistance, and can be used for preparing probiotics for preventing and treating bacteria. Meanwhile, the strain has a relatively strong bacteriostatic effect on escherichia coli, bacillus cereus, staphylococcus aureus and salmonella typhimurium, in addition, the strain has a relatively good degradation capability on nitrite and cholesterol, and the applicability is relatively strong.

The invention belongs to the technical field of microorganisms and genetic engineering, and particularly relates to an alkaline protease mutant and application thereof in leather unhairing. According to the invention, alkaline protease derived from Virgibacillus byunsanensis and alkaline protease derived from Ordinitinibacillus caprae are subjected to directed evolution in vitro, so that an alkaline protease mutant of which the enzyme activity and the stability are remarkably improved is obtained, and active extracellular expression of a coding gene of the mutant is realized in an engineering bacterium bacillus subtilis WB600. The optimal reaction temperature of the alkaline protease mutant is 45 DEG C, the optimal pH value of the alkaline protease mutant is 8, the residual enzyme activity of the alkaline protease mutant can be kept at 90% or above after the alkaline protease mutant is incubated for 12 h under the conditions that the pH is 8 and the temperature is 50 DEG C or below or the temperature is 45 DEG C and the pH is 7-9, and the alkaline protease mutant has good thermal stability and pH stability, has high tolerance to NaCl, is applied to unhairing of salt wet sheepskin subjected to preservative treatment through NaCl with the concentration smaller than or equal to 100 g / L, and has good application prospects. Under the conditions that the pH is 7-9 and the temperature is 40-50 DEG C, the unhairing time is shortened to 1.5-2 hours.

The invention belongs to the technical field of microorganisms, and relates to an aroma-producing yeast XW2-1 and application thereof. The aroma-producing yeast XW2-1 disclosed by the invention is clavipora lusitaniae and is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on June 14, 2024, and the preservation number of the aroma-producing yeast XW2-1 is CGMCC (China General Microbiological Culture Collection Center) No. 30947. The aroma-producing yeast XW2-1 disclosed by the invention is high in growth speed, relatively strong in tolerance and outstanding in aroma-producing effect, and can be used for tobacco aroma enhancement. The strain can ferment tobacco powder in a liquid state, the variety and content of aroma components in fermentation liquor are remarkably increased, and the total amount of the aroma components is increased by two times or above. The tobacco flavor produced by fermentation of the strain has typical aroma characteristics, the aroma enhancement effect is remarkable, the smokeability is high, and the smoking quality of tobacco products can be improved.

The invention discloses a microbial agent for promoting cucumber growth and a preparation method and application thereof, and belongs to the technical field of agricultural microorganisms. The microbial agent is prepared from the following components: achromobacter denitrificans, bradyrhizobium angularis and Vilnelandan nitrogen-fixing bacteria in a volume ratio of 2 to 1 to 1. Bacteria with a growth promoting effect are reasonably combined for use, so that the growth promoting effect of the mixed flora on cucumbers is better than that of a single strain, and the adaptability of the synergistic effect of the strains in the actual soil rhizosphere complex environment is higher. By determining the number of strains and mixing according to a specific proportion, germination of cucumber seeds is stably and effectively promoted, root elongation is promoted, the flowering and fruit setting rate is increased, and meanwhile the plant height, plant biomass and chlorophyll content of cucumbers in the seedling stage are increased. Besides, the microbial agent disclosed by the invention is beneficial to improving the growth quality and yield of plants and reducing environmental pollution, has wide application potential in the fields of agriculture and ecology, and is worthy of popularization.

The invention discloses rahnella and application thereof, and belongs to the technical field of microbiology and biology, the rahnella S5-11 strain provided by the invention is a new species of rahnella, and the preservation number of the rahnella is GDMCC No: 63732. The strain S5-11 has relatively strong tolerance to selenate and selenite, the selenite and the selenite can be reduced into nano elemental selenium, a method for preparing a large amount of nano selenium by utilizing a bioreactor is established, and the strain S5-11 can be used for production of nano selenium and bioremediation of selenium-polluted environment and has a wide application prospect. Besides, the strain has good plant growth promoting ability, can promote crop growth in low-selenium soil, and can relieve stress of selenium on plants in high-selenium soil.

The invention discloses an antibody for resisting HPV16 type E6 protein and E7 protein or an antigen binding fragment of the antibody, an HPV16 detection product and application of the HPV16 detection product, and relates to the technical field of medical biology. The antibody provided by the invention has the advantages of high detection sensitivity, rapidness and strong tolerance on the HPV16 E6 / E7 antigen, can be used for quality control in the production process of the recombinant human HPV16 E6 / E7 antigen, and can efficiently and accurately quantify the antigen content in a vaccine and monitor the quality of the vaccine. And the kit can also be directly used for detecting HPV16 type E6 protein and / or E7 protein.

The invention discloses halomonas for efficiently synthesizing nano-selenium as well as a method for preparing the nano-selenium and application of the halomonas, and belongs to the technical field of biology. The Halomonas sp. CCTCC NO: M 20242266 disclosed by the invention has strong tolerance to high-concentration selenite, can efficiently convert the selenite into nano-selenium, and is simple in required culture condition, low in cost and easy to operate; the nano-selenium particles produced by utilizing the strain disclosed by the invention are stable in property and high in biological activity. The Halomonas sp. CCTCC NO: M 20242266 or a microbial agent of the Halomonas sp. CCTCC NO: M 20242266 or nano-selenium produced by utilizing the strain has wide development prospects in the fields of functional agriculture including planting industry and breeding industry, functional food processing, health food processing, medical product processing and the like.

The invention relates to the technical field of electronic products, and discloses a mounting assembly for a middle frame and electronic equipment. The mounting assembly for the middle frame comprises a mounting piece and an elastic piece, one part of the mounting piece is accommodated in the accommodating groove of the middle frame and is used for mounting a key of the middle frame; and when the mounting piece is accommodated in the accommodating groove, at least one part of the elastic piece is elastically extruded between the mounting piece and the groove side wall of the accommodating groove. According to the mounting assembly provided by the invention, the key can be firmly mounted in the middle frame without shaking, meanwhile, the processing requirement on the size of the middle frame is low, and the mounting assembly is convenient to disassemble and assemble.

The invention discloses an Escherichia coli phage microcapsule causing sheep diarrhea and a preparation method of the Escherichia coli phage microcapsule. The preparation method comprises the following steps: by taking sheep-derived pathogenic escherichia coli as host bacteria, separating from an environmental sewage sample to obtain bacteriophage, and carrying out microencapsulation on the bacteriophage to obtain the bacteriophage microcapsule. Experiments show that the bacteriophage microcapsule has a relatively good treatment effect on sheep-derived pathogenic escherichia coli infection, has relatively strong tolerance and release property to a digestion environment, and has a good application prospect in the aspects of research and development of oral bacteriophage preparations and the like.

The invention discloses a preparation method and application of a pyrazole compound, and relates to a compound with a novel structure and a composition of the compound, and the pyrazole compound shows better activity in vivo and does not show obvious toxicity. The compound provided by the invention can be developed into drugs for protecting multiple diseases such as nerve cells, cardiovascular diseases and the like. # imgabs0 #

The invention belongs to the technical field of high polymer material preparation, and relates to a preparation method and application of antibacterial transparent nylon. The preparation method comprises the following steps: adding nylon, a cross-linking agent and a catalyst into a modified cyclodextrin solution for grafting reaction to obtain cyclodextrin grafted nylon; adding the cyclodextrin grafted nylon into a nano-scale metal ion solution, and then filtering, washing and drying to obtain the antibacterial transparent nylon. According to the invention, a cyclodextrin grafting process is adopted to weaken the crystallinity of the nylon material so as to improve the light transmittance, and meanwhile, nano metal ions are successfully captured and fixed by virtue of a special structure of cyclodextrin, so that the material is endowed with excellent antibacterial performance and does not need to depend on a traditional coupling agent. It is ensured that the nylon material still has good overall performance after antibacterial modification, and a new thought is provided for developing an efficient compatibility technology and exploring and innovating a modification strategy for the nylon material.

The invention belongs to the technical field of microorganisms and aquaculture, and discloses a high-environmental-adaptability nitrification and denitrification bacillus cereus strain, a microbial agent and application of the high-environmental-adaptability nitrification and denitrification bacillus cereus strain, the high-environmental-adaptability nitrification and denitrification bacillus cereus strain is bacillus cereus HCCN1 and is preserved in Guangdong Microbial Culture Collection Center on November 13, 2024, and the preservation number is GDMCC No: 65477. The invention also discloses a microbial agent containing the strain HCCN1 as an active component and application of the microbial agent. The strain HCCN1 has high stress resistance and high environmental adaptability, has heterotrophic nitrification and aerobic denitrification functions at the same time, can efficiently degrade nitrogen and phosphorus under the severe environmental conditions of wide pH value range, temperature range, high salt and alkali concentration and the like, performs purification treatment on aquaculture water, and is high in biological safety and low in cost. The industrial requirements of efficient, healthy and sustainable aquaculture can be met.

The invention relates to the field of solar cells, and discloses a repair method and an evaluation method for edge cutting loss of a solar cell. Firstly, according to the repairing method, ozone water is adopted for cooling the cut surface of the silicon wafer subjected to laser treatment, and the cut surface can be heated to release oxygen while being cooled, so that an oxide layer is formed on the cut surface of the silicon wafer, and the cut surface of the silicon wafer is protected in time; the sulfonic acid compound mixed solution with the hydrogen peroxide as the solvent serves as the organic / inorganic composite passivation solution, and compared with a conventional passivation film, a formed passivation film is higher in high temperature resistance and better in passivation durability. Secondly, according to the evaluation method disclosed by the invention, various environment and test error factors except experimental conditions of the same silicon wafer can be excluded in the test process, meanwhile, the test time and the use amount of sample wafers can be reduced, and a more accurate test result can be obtained in a shorter time.

The invention discloses an active polypeptide of protoporphyrinogen oxidase and application, the active polypeptide not only has the activity of the protoporphyrinogen oxidase, but also has better tolerance to PPO inhibitor herbicides by transferring a nucleotide sequence for coding the active polypeptide. The herbicide tolerance is wide. The active polypeptide disclosed by the invention shows relatively high tolerance to PPO inhibitor herbicides, so that the active polypeptide has a wide application prospect in plants. The herbicide tolerance is high. The active polypeptide disclosed by the invention has strong tolerance to a PPO inhibitor herbicide, and shows high tolerance to saflufenacil, sulfentrazone, flumioxazin and lactofen which are 4 times of field concentration. The weed control method provided by the invention can effectively reduce the occurrence of resistant weeds, has broad-spectrum herbicide resistance, effectively reduces the cost, improves the control efficiency, and also can greatly avoid the occurrence of weed resistance.

The invention relates to the technical field of nuclear fuel, and particularly discloses a cooperative flow control high-reliability fuel assembly which comprises a lower pipe seat, a framework and an upper pipe seat which are sequentially arranged in the flowing direction of a coolant, and a fuel rod, a guide pipe and an instrument pipe which are inserted into the framework, the lower tube seat is a low-outlet transverse flow down tube seat; a plurality of axially bent space through slender runners with the width smaller than the distance between the fuel rods are arranged on the lower tube seat connecting plate; and a peripheral flow channel area outlet of the space through slender flow channel, which is positioned at the periphery of the lower tube seat connecting plate, is higher than a middle flow channel area outlet in the middle of the lower tube seat connecting plate, so that outlet transverse flow between assemblies is reduced. According to the invention, collaborative innovation design is carried out on the whole fuel assembly, and the high thermotechnical safety margin of the fuel assembly is guaranteed.

The invention discloses enterococcus faecalis YL-EF32 and application thereof, and belongs to the technical field of microorganisms. The collection number of the enterococcus faecalis YL-EF32 provided by the invention is CCTCC (China Center For Type Culture Collection) NO: M 20242241. The strain can effectively inhibit the proliferation of chicken-derived clostridium perfringens, has better tolerance to cholate, artificial gastric juice and phenol, and can metabolize dietary fibers such as pectin, glucan, cellobiose and the like. Enterococcus faecalis YL-EF32 is added into daily ration of broiler chickens, necrotic enteritis induced by coccidiosis and clostridium perfringens can be remarkably improved by means of enhancing intestinal barriers, balancing intestinal flora stability, relieving inflammatory response and the like, and the enterococcus faecalis YL-EF32 can be used for resisting clostridium perfringens infection in livestock and poultry breeding.

The invention relates to the technical field of application of bioremediation microbial agents to environmental remediation, and particularly provides a bioremediation microbial agent containing rhodococcus and application of the bioremediation microbial agent. The bioremediation microbial agent comprises rhodococcus fermentation liquor and a stabilizer. The bioremediation microbial agent is used for degrading cyanide, can be particularly used for degrading cyanide in sewage and soil containing various cyanide and / or high-concentration cyanide, has the degradation efficiency obviously higher than that of a traditional chemical remediation agent, does not cause secondary environmental pollution, is more environment-friendly and has stable performance.

The present invention relates to the field of nuclear fuel technology, and specifically discloses a high-reliability fuel assembly with coordinated flow control, including a lower tube seat, a skeleton, an upper tube seat, and fuel rods, guide tubes, and instrument tubes inserted into the skeleton, which are sequentially arranged along the flow direction of the coolant; the lower tube seat is a low-outlet lateral flow lower tube seat; on the lower tube seat connecting plate, there are a number of space-through slender flow channels with a width less than the fuel rod spacing and axial bending; the outlets of the surrounding flow channel areas of the space-through slender flow channels located on the outer periphery of the lower tube seat connecting plate are higher than the outlets of the middle flow channel areas located in the middle of the lower tube seat connecting plate, so as to reduce the lateral flow of the outlets between assemblies. The present invention carries out a coordinated and innovative design of the fuel assembly as a whole, and ensures a high thermal safety margin of the fuel assembly.

The invention discloses a chicken circular RNA (Ribonucleic Acid) for promoting chicken primary myoblast proliferation and application of the chicken circular RNA, the chicken circular RNA is gal-SVIL0013, and the invention also provides a detection primer of the chicken circular RNA. According to the present invention, the existence of the annular transcript gal-SVIL0013 of the NFIA is found, and the strong tolerance of the gal-SVIL0013 on the RNase R excision enzyme is detected at the same time; fluorescent quantitative PCR analysis finds that gal-SVIL0013 has no obvious differential expression in pectoralis of 11, 16 and 18 embryonic ages of Dalian pheasants, and the expression of gal-SVIL0013 in 11, 16 and 18 embryonic ages of white feather broilers is significantly increased. In the primary myoblast of the chicken, the gal-SVIL0013 can be used for promoting the proliferation of the cells.

The utility model relates to a leakage-proof water-blocking through-wall wiring device, which comprises two waterproof clamping plates and a long-axis bolt, wherein a connecting hole is formed in the center of each waterproof clamping plate; a long-axis bolt penetrates through the connecting hole in the center of one waterproof clamping plate, the through-wall hole of the wall body and the two ends of the connecting hole in the center of the other waterproof clamping plate, and the inner side disc faces of the waterproof clamping plates are compacted on the wall face through nuts; a patch board is arranged in an inner area and an outer area around a center hole of the waterproof clamping plate, a plurality of binding posts are distributed on the patch board and penetrate through the patch board, only binding posts are reserved on the inner side and the outer side of the waterproof clamping plate, a protective cover is arranged outside the waterproof clamping plate, and a cable threading hole is formed in the protective cover. According to the utility model, the requirements of leakage prevention and water resistance can be met, and the construction amount can be reduced.

The invention discloses a high-efficiency liquid organic fertilizer and a preparation method thereof, and belongs to the technical field of organic fertilizer. It is made of the following raw materials in parts by weight: 200-300 parts of livestock and aquaculture wastewater, 50-80 parts of plant straw, 10-20 parts of pretreatment agent, 10-20 parts of EM bacteria, 20-30 parts of composite microbial agent, 3-10 parts of promoter, 1-3 parts of potassium dihydrogen phosphate, and 0.05-0.2 parts of trace elements; the promoter is composed of sunflower disk extract and sodium lauryl alcohol polyether sulfate in a mass ratio of 3:1; the pretreatment agent is a biochar-TiO2 composite material. The present invention realizes the resource utilization of livestock and aquaculture wastewater, converts wastewater into liquid organic fertilizer rich in nutrient elements, provides high-quality fertilizer resources for agricultural production, effectively removes harmful substances in wastewater through the combination of biological treatment and physical and chemical treatment technology, ensures the safety and environmental protection of organic fertilizer, and the obtained organic liquid fertilizer has a significant effect on improving potato yield and quality, and has good application prospects.