11 results about "Cupriavidus" patented technology

A process for producing polyhydroxyalkanoates, comprising the steps of: fermenting production medium comprising pongamia oil using bacteria from the Cupriavidus, Burkholderia, or Alcaligenes genera capable of producing polyhydroxyalkanoates; wherein the pongamia oil is extracted from the seeds of Pongamia species and clarified by filtering before the crude, unprocessed pongamia oil is added to the production medium; and extracting the polyhydroxyalkanoates comprising a homopolymer comprising a 3-hydroxybutyrate (3HB) unit amongst other sole PHB polymers from within the bacteria.

The application provides a phenol degradation method, comprising the following steps: inoculating Cupriavidus basellens in a first culture medium with a phenol concentration of 200-800 mg / L, culturing at a temperature of 26-36 DEG C under a rotating speed of 120-150 rpm, and realizing phenol degradation through lysis growth of the Cupriavidus basellens; wherein the initial volume ratio of the Cupriavidus basellens to the first culture medium is 5-10%, and the pH value of the first culture medium is 5-11. The application can efficiently and highly degrade phenol, has a remarkable effect, and is worth promoting.

The application discloses cupriavidus capable of producing polyhydroxyalkanoate by using various carbon sources such as ethanol and application thereof. Cupriavidus The strain sp.ZWJ01 has a preservation number of CGMCC No.37462 in the China General Microbiological Culture Collection Center. The strain can grow and accumulate poly-3-hydroxybutyric acid ester by using various substances such as ethanol, volatile fatty acid and sugar as carbon sources; can grow by using mixed carbon sources of ethanol+propionic acid or ethanol+valeric acid and accumulate poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid ester) in cells; and can grow by using mixed carbon sources of ethanol+gamma-butyrolactone or ethanol+1,4-butanediol and accumulate poly(3-hydroxybutyric acid-co-4-hydroxybutyric acid) in cells, and has a good application prospect.

The present invention relates to the field of aerobic gas-substrate fermentation, in particular for Cupriavidus through a fully flooded vertical loop reactor system. The present invention further relates to a fully flooded vertical loop bioreactor (100) for aerobic gas-substrate fermentation. The present invention further relates to a method for aerobic gas-substrate fermentation using the fully flooded vertical loop bioreactor (100) of the invention.

The application discloses cupriavidus CUP3, a combination of cupriavidus CUP3 and raoultella ornithinolytica RAO1 and application of the combination in prevention and control of crop bacterial wilt. Cupriavidus necator Cupriavidus pinatubonensis Cupri The application discloses cupriavidus CUP3, a combination of cupriavidus CUP3 and raoultella ornithinolytica RAO1 and application of the combination in prevention and control of crop bacterial wilt. The application discloses cupriavidus CUP3, a combination of cupriavidus CUP3 and raoultella ornithinolytica RAO1 and application of the combination in prevention and control of crop bacterial wilt. The application discloses cupriavidus CUP3, a combination of cupriavidus CUP3 and raoultella ornithinolytica RAO1 and application of the combination in prevention and control of crop bacterial wilt.

This invention discloses a composition of *Brachystomum clavatum* CUP3, *Brachystomum clavatum* CUP3, and *Raoultella ornithine-lysinogen* RAO1, and its application in controlling bacterial wilt in crops. *Brachystomum clavatum* CUP3 is classified as... Cupriavidus yeoncheonensis The invention discloses the application of *Cup3*, a copper-loving bacterium, in the control of soil-borne bacterial wilt in crops. The invention also discloses a bacterial composition containing *Cup3* and *Rauvolfia ornithine-releasing* RAO1, or a fermentation broth containing *Cup3* and *Rauvolfia ornithine-releasing* RAO1; wherein the preservation number of *Rauvolfia ornithine-releasing* RAO1 is GDMCC NO:67513. Both *Cup3* and the bacterial composition can reduce the occurrence of bacterial wilt in tomatoes, especially the bacterial composition, which can significantly reduce the occurrence of bacterial wilt in tomatoes.

The present application relates to the field of agricultural microorganisms and heavy metal pollution prevention, and provides an acid-stress-tolerant metal-tolerant cupriavidus strain, application and microbial agent thereof.The strain is preserved in the China General Microbiological Culture Collection Center, has the acid-tolerant property of stable growth in a pH 3.0 to 7.0 environment, and has the ability to reduce the concentration of various heavy metal ions such as chromium, manganese, copper, arsenic, cadmium and lead.In the wheat and rice hydroponic germination test, the strain can significantly promote root growth and alleviate the inhibition of various heavy metal stresses on root development, and the heavy metal ion removal rate can reach a high level.The present application can be used for heavy metal pollution treatment and agricultural green and safe production under acidic conditions.

The invention belongs to the technical field of rice field cadmium pollution treatment, and particularly relates to a multi-dimensional cadmium-control ecological regulating agent and application thereof in rice planting in cadmium-polluted soil. The multi-dimensional cadmium-controlling ecological regulating agent comprises waste activated carbon containing micromolecule polypeptide, macromolecular polypeptide, molasses and a rice endophyte solution, the rice endophyte solution is rice endophytic metal-resistant cupriavidus CML2, and the volume L of the rice endophyte solution is 1-5% of the volume L of the rice endophytic metal-resistant cupriavidus CML2. The ratio of the waste activated carbon containing the micromolecule polypeptide to the macromolecular polypeptide to the molasses to the rice endophyte solution is 16: 32: 1: 1. The multi-dimensional cadmium-controlling ecological regulating agent provided by the invention can reduce cadmium in a multi-dimensional manner by means of soil in-situ passivation, micro-ecological combination, rice endophyte prevention and control, porous activated carbon adsorption and the like, so that yield increase and restoration of rice are effectively realized at the same time, and the cadmium content of the produced rice is lower than the national highest limit standard 0.2 mg / kg.

This invention discloses a chemoautotrophic alkaloid-producing strain of *Alcaligenes rocheborella*, its construction method, and its application. The strain is based on the chemoautotrophic microorganism *Alcaligenes rocheborella* H16 (… Cupriavidus necator Using H16 as the starting strain, the poly-3-hydroxybutyrate (PHB) synthesis pathway of the strain was first knocked out, and then a strain from Rhodotorula glutinis (H16) was introduced. Rhodotorula glutinis ) tyrosine ammonia lyase gene tal A basic engineered strain capable of producing p-coumaric acid was constructed. Subsequently, the throughput of the p-coumaric acid synthesis pathway in the engineered strain was enhanced by overexpressing the endogenous 3-deoxy-7-phosphate heptanulate synthase gene aroG1 and the branching acid synthase gene aroC, and by increasing the tal copy number. Further enhancement was achieved by introducing strains from *Escherichia coli* (…). Escherichia coli By improving the supply of cofactors through the NAD(P) transhydrogenase gene pntAB, a strain of Alcaligenes roximatelye that can synthesize p-coumaric acid using CO2 was finally obtained.

The present application belongs to the technical field of microorganisms and their applications, and particularly relates to a low-temperature-resistant benzofluor degrading bacterium and application thereof. The strain is Cupriavidus, and was preserved in the China General Microbiological Culture Collection Center on July 22, 2025, with a preservation number of CGMCC No. 35332. The present application can accelerate the benzofluor degradation process under low-temperature conditions, and is helpful to enrich the microbial treatment means for benzofluor residue degradation in farmland soil. The present application can effectively degrade benzofluor under low-temperature conditions by using the purified strain CS1 to prepare an efficient pesticide degrading bacterium, promote pesticide residue degradation in farmland soil, and reduce the migration risk of pesticides to crops, groundwater and humans. Compared with traditional methods such as soil thermal desorption, chemical oxidation and guest soil method, the present application has higher efficiency, lower cost and is easier to operate, and provides an effective microbial technical solution for the degradation of benzofluor under low-temperature conditions.

The invention discloses a hymexazol degrading gene cluster, an expression vector of the hymexazol degrading gene cluster, hymexazol degrading bacteria and application of the hymexazol degrading bacteria. The gene cluster comprises an orf2179 gene with a DNA (Deoxyribose Nucleic Acid) sequence as shown in SEQ ID No. 1, an orf2181 gene with a DNA sequence as shown in SEQ ID No. 3 and an orf2182 gene with a DNA sequence as shown in SEQ ID No. 4. An expression vector and recombinant bacteria containing the gene cluster are further constructed, the degradation function of the gene cluster on the hymexazol is verified, and the result shows that the orf2180 gene does not participate in the degradation effect, but the orf2179 gene, the orf2181 gene and the orf2182 gene participate in the preliminary degradation of the hymexazol, and the results are indispensable. The Cupriavidus recombinant bacterium for expressing the orf2174-2183 gene cluster, which is constructed in the invention, has better hymexazol degradation performance and can be used for efficiently repairing a hymexazol polluted environment.