20 results about "Cupriavidus" patented technology

The invention belongs to the technical field of biological phosphorus-silicon fertilizers, and particularly relates to a liquid complex microbial inoculant capable of repairing heavy metals and activating phosphorus and silicon and a biological phosphorus-silicon fertilizer thereof. The liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria Cupriavidus and Ochrobactrum, nitrogen-fixing bacteria Azospirillum brasilense, phosphate solubilizing bacteria Bacillus megaterium and Pseudomonas, potassium solubilizing bacteria Bacillus mucilaginosus, growth-promoting bacteria Pseudomonas fluorescens, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants and microbial inoculants. The biocontrol bacteria are trichoderma harzianum and bacillus subtilis, and the synergistic bacteria are saccharomyces cerevisiae; the biological phosphorus-silicon fertilizer is prepared from the liquid complex microbial inoculant. According to the biological phosphorus-silicon fertilizer provided by the invention, through scientific compatibility of various functional strains, heavy metal remediation and soil phosphorus and silicon solubilization are realized, absorption of phosphorus and silicon in soil is promoted, and the crop yield is increased.

The invention discloses a Rogowski eutrophy alkali-producing engineering strain for producing p-coumaric acid as well as a construction method and application of the Rogowski eutrophy alkali-producing engineering strain. The preparation method comprises the following steps: by taking a chemoautotrophic microorganism Ropriavidus alcaligenes H16 as an original strain, firstly, by knocking out a synthetic route of a strain poly-3-hydroxybutyrate (PHB), introducing a tyrosine ammonia lyase gene from Rhodotorula glutinis to construct a basic engineering strain capable of producing p-coumaric acid, and then, carrying out fermentation on the basic engineering strain to obtain the p-coumaric acid. Then, endogenous 3-deoxy-7-phosphoheptanone acid synthase gene aroG1 and chorismate synthase gene aroC are overexpressed, and the total copy is increased, so that the p-coumaric acid synthetic pathway flux of the engineering strain is enhanced; and introducing NAD (P) transhydrogenase gene pntAB from Escherichia coli to improve the supply of cofactors, so as to finally obtain the eutrophy alcaligenes Rosei engineering strain capable of synthesizing p-coumaric acid by using CO2.

A transformed microorganism having an ability to produce a polyhydroxyalkanoate includes a polyhydroxyalkanoate synthase gene and a gene encoding a chaperone belonging to the ClpB family. The gene encoding the chaperone belonging to the ClpB family is a gene which has been introduced into the transformed microorganism or whose expression has been enhanced in the transformed microorganism. The chaperone belonging to the ClpB family may be derived from the genus Cupriavidus, Escherichia, or Saccharomyces. A polyhydroxyalkanoate can be produced by culturing the transformed microorganism.

The invention relates to the field of agricultural microorganism and heavy metal pollution prevention and control, and provides an acid-stress-resistant metal-resistant cupriavidus strain, application thereof and a fungicide. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36356, and the strain has the acid-resistant characteristic of stable growth in an environment with the pH value of 3.0-7.0, and has the capability of reducing the concentration of various heavy metal ions such as chromium, manganese, copper, arsenic, cadmium, lead and the like. In a wheat and rice water culture germination test, the strain can remarkably promote root growth and relieve the inhibition effect of stress of various heavy metals on root development, and the removal rate of heavy metal ions can reach a relatively high level. The method can be used for heavy metal pollution treatment and agricultural green and safe production under the acidic condition.

A process for producing polyhydroxyalkanoates, comprising the steps of: fermenting production medium comprising pongamia oil using bacteria from the Cupriavidus, Burkholderia, or Alcaligenes genera capable of producing polyhydroxyalkanoates; wherein the pongamia oil is extracted from the seeds of Pongamia species and clarified by filtering before the crude, unprocessed pongamia oil is added to the production medium; and extracting the polyhydroxyalkanoates comprising a homopolymer comprising a 3-hydroxybutyrate (3HB) unit amongst other sole PHB polymers from within the bacteria.

The invention belongs to the technical field of microorganisms and application thereof, and particularly relates to a low-temperature-resistant toprazole fluridone degrading bacterium and application thereof. The strain is Cupriavidus, the Cupriavidus is preserved in the China General Microbiological Culture Collection Center on July 22, 2025, and the preservation number of the Cupriavidus is CGMCC (China General Microbiological Culture Collection Center) No.35332. The strain and the fungicide can accelerate the degradation process of the toprazole fluridone under a low-temperature condition, and are beneficial to enriching microbiological treatment means for degrading toprazole fluridone residues in farmland soil. According to the present invention, the purified strain CS1 is prepared into the efficient pesticide degradation bacterial agent, such that the topramezone can be effectively degraded in the low temperature environment, the degradation of the pesticide residue in the farmland soil is promoted, and the migration risk of the pesticide to crops, underground water and human beings is reduced. Compared with traditional methods such as soil thermal desorption, chemical oxidation and soil dressing methods, the method is higher in efficiency, lower in cost and easier to operate, and an effective microbial technical solution is provided for degradation of the topramezone under the low-temperature condition.

The invention discloses a flora combination for in-situ enrichment of earthworms in soil, a culture medium and application of the flora combination, and the flora combination comprises Cupriavidus necator, a bacterial strain YTR-C1 with the preservation number of CGMCC27358, Sinorhizobium sp., a bacterial strain YTR-S1 with the preservation number of CGMCC20681, Mesorhizobium amorphae and a bacterial strain YTR-M3 with the preservation number of CGMCC17532, and the bacterial strain YTR-C1 with the preservation number of CGMCC27358, Sinorhizobium sp., Sinorhizobium sp., Sinorhizobium sp., Sinorhizobium sp., Sinorhizobium sp., Sinorhizobium sp., the flora composition is preserved in China General Microbiological Culture Collection Center on May 16th, 2023, September 18th, 2020 and April 9th, 2019, the flora composition and the culture medium can increase the number of the far-caffeilus in the red soil, field tests find that the effective phosphorus content and the phosphatase activity in the red soil are remarkably increased by adding the far-caffeilus, and the effective phosphorus content and the phosphatase activity of the red soil are remarkably increased by adding the far-caffeilus. The yield and the quality of the corn and the rape are improved.

The application provides a phenol degradation method, comprising the following steps: inoculating Cupriavidus basellens in a first culture medium with a phenol concentration of 200-800 mg / L, culturing at a temperature of 26-36 DEG C under a rotating speed of 120-150 rpm, and realizing phenol degradation through lysis growth of the Cupriavidus basellens; wherein the initial volume ratio of the Cupriavidus basellens to the first culture medium is 5-10%, and the pH value of the first culture medium is 5-11. The application can efficiently and highly degrade phenol, has a remarkable effect, and is worth promoting.

The application discloses cupriavidus capable of producing polyhydroxyalkanoate by using various carbon sources such as ethanol and application thereof. Cupriavidus The strain sp.ZWJ01 has a preservation number of CGMCC No.37462 in the China General Microbiological Culture Collection Center. The strain can grow and accumulate poly-3-hydroxybutyric acid ester by using various substances such as ethanol, volatile fatty acid and sugar as carbon sources; can grow by using mixed carbon sources of ethanol+propionic acid or ethanol+valeric acid and accumulate poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid ester) in cells; and can grow by using mixed carbon sources of ethanol+gamma-butyrolactone or ethanol+1,4-butanediol and accumulate poly(3-hydroxybutyric acid-co-4-hydroxybutyric acid) in cells, and has a good application prospect.

To provide alternative protein-rich food products that are not derived from animals, but are free of unhealthy meat-related components such as saturated fatty acids and cholesterol, are free of the deleterious environmental effects of animal husbandry, and provide similar texture and flavor characteristics and functional properties as animal meat.SOLUTION: Structured food compositions, such as meat substitute compositions, comprising microbial-derived protein products, such as microbial-derived protein hydrolysates, are provided. Methods of making such products are also provided. Also provided are flavor potentiator compositions comprising heme-containing polypeptides, such as microbial-derived flavohemoglobin, and food compositions, such as meat substitute compositions, comprising the heme-containing flavor potentiator compositions. In one aspect, the flavor potentiator comprises a heme-containing polypeptide from a hydrogen-oxidizing microorganism, wherein (i) the hydrogen-oxidizing microorganism is a Cupriavidus microorganism or (ii) the hydrogen-oxidizing microorganism grows under microaerobic conditions.SELECTED DRAWING: None

The present invention relates to the field of aerobic gas-substrate fermentation, in particular for Cupriavidus through a fully flooded vertical loop reactor system. The present invention further relates to a fully flooded vertical loop bioreactor (100) for aerobic gas-substrate fermentation. The present invention further relates to a method for aerobic gas-substrate fermentation using the fully flooded vertical loop bioreactor (100) of the invention.

The application discloses cupriavidus CUP3, a combination of cupriavidus CUP3 and raoultella ornithinolytica RAO1 and application of the combination in prevention and control of crop bacterial wilt. Cupriavidus necator Cupriavidus pinatubonensis Cupri The application discloses cupriavidus CUP3, a combination of cupriavidus CUP3 and raoultella ornithinolytica RAO1 and application of the combination in prevention and control of crop bacterial wilt. The application discloses cupriavidus CUP3, a combination of cupriavidus CUP3 and raoultella ornithinolytica RAO1 and application of the combination in prevention and control of crop bacterial wilt. The application discloses cupriavidus CUP3, a combination of cupriavidus CUP3 and raoultella ornithinolytica RAO1 and application of the combination in prevention and control of crop bacterial wilt.

The present application relates to the field of microbial technology, and particularly relates to a rhizosphere basing cupriavidus basellens - 10 and application thereof in dissolving phosphorus and promoting growth. The rhizosphere basing cupriavidus basellens - 10 provided by the present application has a preservation number of GDMCC No. 65501, and the rhizosphere basing cupriavidus basellens - 10 can increase the contents of chlorophyll a, chlorophyll b, carotenoids and total chlorophyll in leaves of cinnamomum chekiangense seedlings, increase the plant height, ground diameter, total fresh weight, root fresh weight, stem fresh weight, leaf fresh weight, total root length and thick root number of the cinnamomum chekiangense seedlings, and promote the accumulation of soluble sugar and effective phosphorus in roots and leaves.

This invention discloses a composition of *Brachystomum clavatum* CUP3, *Brachystomum clavatum* CUP3, and *Raoultella ornithine-lysinogen* RAO1, and its application in controlling bacterial wilt in crops. *Brachystomum clavatum* CUP3 is classified as... Cupriavidus yeoncheonensis The invention discloses the application of *Cup3*, a copper-loving bacterium, in the control of soil-borne bacterial wilt in crops. The invention also discloses a bacterial composition containing *Cup3* and *Rauvolfia ornithine-releasing* RAO1, or a fermentation broth containing *Cup3* and *Rauvolfia ornithine-releasing* RAO1; wherein the preservation number of *Rauvolfia ornithine-releasing* RAO1 is GDMCC NO:67513. Both *Cup3* and the bacterial composition can reduce the occurrence of bacterial wilt in tomatoes, especially the bacterial composition, which can significantly reduce the occurrence of bacterial wilt in tomatoes.

The present application relates to the field of agricultural microorganisms and heavy metal pollution prevention, and provides an acid-stress-tolerant metal-tolerant cupriavidus strain, application and microbial agent thereof.The strain is preserved in the China General Microbiological Culture Collection Center, has the acid-tolerant property of stable growth in a pH 3.0 to 7.0 environment, and has the ability to reduce the concentration of various heavy metal ions such as chromium, manganese, copper, arsenic, cadmium and lead.In the wheat and rice hydroponic germination test, the strain can significantly promote root growth and alleviate the inhibition of various heavy metal stresses on root development, and the heavy metal ion removal rate can reach a high level.The present application can be used for heavy metal pollution treatment and agricultural green and safe production under acidic conditions.

The invention belongs to the technical field of rice field cadmium pollution treatment, and particularly relates to a multi-dimensional cadmium-control ecological regulating agent and application thereof in rice planting in cadmium-polluted soil. The multi-dimensional cadmium-controlling ecological regulating agent comprises waste activated carbon containing micromolecule polypeptide, macromolecular polypeptide, molasses and a rice endophyte solution, the rice endophyte solution is rice endophytic metal-resistant cupriavidus CML2, and the volume L of the rice endophyte solution is 1-5% of the volume L of the rice endophytic metal-resistant cupriavidus CML2. The ratio of the waste activated carbon containing the micromolecule polypeptide to the macromolecular polypeptide to the molasses to the rice endophyte solution is 16: 32: 1: 1. The multi-dimensional cadmium-controlling ecological regulating agent provided by the invention can reduce cadmium in a multi-dimensional manner by means of soil in-situ passivation, micro-ecological combination, rice endophyte prevention and control, porous activated carbon adsorption and the like, so that yield increase and restoration of rice are effectively realized at the same time, and the cadmium content of the produced rice is lower than the national highest limit standard 0.2 mg / kg.

This invention discloses a chemoautotrophic alkaloid-producing strain of *Alcaligenes rocheborella*, its construction method, and its application. The strain is based on the chemoautotrophic microorganism *Alcaligenes rocheborella* H16 (… Cupriavidus necator Using H16 as the starting strain, the poly-3-hydroxybutyrate (PHB) synthesis pathway of the strain was first knocked out, and then a strain from Rhodotorula glutinis (H16) was introduced. Rhodotorula glutinis ) tyrosine ammonia lyase gene tal A basic engineered strain capable of producing p-coumaric acid was constructed. Subsequently, the throughput of the p-coumaric acid synthesis pathway in the engineered strain was enhanced by overexpressing the endogenous 3-deoxy-7-phosphate heptanulate synthase gene aroG1 and the branching acid synthase gene aroC, and by increasing the tal copy number. Further enhancement was achieved by introducing strains from *Escherichia coli* (…). Escherichia coli By improving the supply of cofactors through the NAD(P) transhydrogenase gene pntAB, a strain of Alcaligenes roximatelye that can synthesize p-coumaric acid using CO2 was finally obtained.

The present application belongs to the technical field of microorganisms and their applications, and particularly relates to a low-temperature-resistant benzofluor degrading bacterium and application thereof. The strain is Cupriavidus, and was preserved in the China General Microbiological Culture Collection Center on July 22, 2025, with a preservation number of CGMCC No. 35332. The present application can accelerate the benzofluor degradation process under low-temperature conditions, and is helpful to enrich the microbial treatment means for benzofluor residue degradation in farmland soil. The present application can effectively degrade benzofluor under low-temperature conditions by using the purified strain CS1 to prepare an efficient pesticide degrading bacterium, promote pesticide residue degradation in farmland soil, and reduce the migration risk of pesticides to crops, groundwater and humans. Compared with traditional methods such as soil thermal desorption, chemical oxidation and guest soil method, the present application has higher efficiency, lower cost and is easier to operate, and provides an effective microbial technical solution for the degradation of benzofluor under low-temperature conditions.

The application discloses a strain of Cupriavidus sp. and application thereof in degrading benzohydroxamic acid. The application separates a BHA degrading bacterium, Cupriavidus sp. HY21, from soil in a mining area, and the bacterium is preserved in the Guangdong Provincial Microbial Culture Collection Center on September 11, 2024, with a preservation number of GDMCC No: 65117. The optimal degrading condition is that the pH is 5.5, the temperature is 30.5 DEG C, and the inoculation amount is 1.0 (OD600nm). Under the condition, the degrading rate of 100 mg / L BHA can reach more than 97 % only in 24 hours. In addition, the bacterium also has good degrading capacity for hydroxamic acid collectors and benzoic acid, salicylic acid, phenol and / or o-diphenol, and can be used for BuX pollution remediation.

The invention discloses a hymexazol degrading gene cluster, an expression vector of the hymexazol degrading gene cluster, hymexazol degrading bacteria and application of the hymexazol degrading bacteria. The gene cluster comprises an orf2179 gene with a DNA (Deoxyribose Nucleic Acid) sequence as shown in SEQ ID No. 1, an orf2181 gene with a DNA sequence as shown in SEQ ID No. 3 and an orf2182 gene with a DNA sequence as shown in SEQ ID No. 4. An expression vector and recombinant bacteria containing the gene cluster are further constructed, the degradation function of the gene cluster on the hymexazol is verified, and the result shows that the orf2180 gene does not participate in the degradation effect, but the orf2179 gene, the orf2181 gene and the orf2182 gene participate in the preliminary degradation of the hymexazol, and the results are indispensable. The Cupriavidus recombinant bacterium for expressing the orf2174-2183 gene cluster, which is constructed in the invention, has better hymexazol degradation performance and can be used for efficiently repairing a hymexazol polluted environment.