31 results about "Cupriavidus" patented technology

The invention relates to a heterotrophic nitrification-aerobic denitrification compound bacterial agent for low-temperature high ammonia-nitrogen removal and application. The compound bacterial agentis formed by compounding of 10-20% of cupriavidus SWA1, 5-20% of alcaligenes faecalis, 10-30% of acinetobacter and 20-50% of ochrobacterum TAC-2. The compound bacterial agent has advantages of high ammonia-nitrogen resistance, low-temperature resistance, heavy metal resistance and synchronous nitrification and denitrification; by synergistic effects of four microorganisms, efficient denitrification of high ammonia-nitrogen wastewater can be realized in an aerobic environment under low-temperature conditions.

The invention relates to a cupriavidus sp. IDO capable of aerobic degrading indole and the application thereof, and belongs to the field of microbial biotechnology. The bacterial strain is separated from soil samples at the seaside of Black Reef in Dalian City, Liaoning Province, China is preserved in the China General Microbiological Culture Collection Center, CGMCC on June 4th, 2014, and the preservation number of the bacterial strain is CGMCC No.9265. The bacterial strain IDO is a Gram-negative bacterium and is in a short-rod shape, and through the determination of a 16SrRNA gene sequence, the bacterial strain IDO and the cupriavidus have higher likeness, so that the bacterium is classified and named as Cupriavidus sp. IDO, and the GenBank accession number of the16SrRNA gene sequence of the bacterial strain is KJ875862. The bacterial strain uses various aromatic compounds as a unique carbon source for growth, has a higher degrading capacity to the indole, also has better pH and temperature tolerance, and shows the application prospects in the treatment of wastewater containing high-concentration indole.

The invention discloses a method for preparing polyhydroxybutyrate (PHB), which is implemented by taking cupriavidus strains sh-1 (preservation No.: CGMCC No. 4815) as strains for preparing PHB. The method comprises the following steps: inoculating the strains in a slant culture medium, then culturing the strains for 24 hours at a temperature of 30 DEG C; taking slant cultures, then inoculating the slant cultures in a conical flask filled with a seed culture medium, and under the condition of shaking the conical flask at a rotating speed of 180 r/min, culturing the slant cultures for 24-36 hours at a temperature of 30 DEG C; inoculating the obtained seed liquid in a fermentation medium according to an inoculation quantity of 10%, and stirring the obtained product at a rotating speed of 300-500 r/min, then after fermenting the seed liquid 72 hours at a ventilatory capacity of 50-200 L/min and a fermentation temperature of 28-35 DEG C, stopping the operation of fermentation; and in the process of fermentation, when the dissolved oxygen content of the fermentation medium is increased to 80% of saturated dissolved oxygen content, adding a glucose solution (concentration: 10-300 g/L) into the fermentation medium, then when the dissolved oxygen content of the obtained solution drops to 50%, stopping the operation of adding. By using the method disclosed by the invention, the high-density fermentation and high yield of polyhydroxybutyrate can be realized.

Escherichia coli for detecting lead is disclosed. Escherichia coli for detecting lead is disclosed. The invention provides a construction method of an escherichia coli engineered strain for detecting heavy metal lead and establishment of a method for detecting lead in a water body. The gene engineering strain is PpbrA-pbrR-PpbrA::DsRed-express2/E.coli delta zntR delta zntA, named as E.coli WMC-008p CGMCC No.9748, wherein zntA and zntR genes undergo deletion mutation so as to be inactivated, and a pbr operon originated from a Cupriavidus metallidurans CH34 strain is knocked-in to express the red fluorescence protein DsRed-express2. In comparison with a reporter plasmid-containing E. coli reporter strain, the engineering strain provided by the invention has advantages of simple operation, low fluorescence background value, stable signal and the like. The engineering strain can reflect bio-availability of lead in a water body and provides a basis for objective evaluation of bio-toxicity effects of lead in a water body.

The invention discloses a cupriavidus strain capable of transforming heavy metals and application of the strain. The strain is named as cupriavidus metallidurans Paddy-2, which is preserved in China General Microbiological Culture Collection Center of Institute of Microbiology of Chinese Academy of Sciences on No.3, No. 1 Yard, West Beichen Road, Chaoyang District, Beijing on June 9, 2015, with preservation number of CGMCC No. 11028. The strain, which has a relatively strong ferrous oxidization capacity and is capable of achieving mineralization by ferrous oxidization in a nitrate environment, is applicable to soil improvement; and the strain also has a relatively strong reduction effect on arsenic and the reduction effect on the arsenic is stronger under a condition free from nitrate and ferrous ions; therefore, the strain is suitable for repairing arsenic-contaminated soil.

The invention provides Cupriavidus alkaliphilus (Cupriavidus alkaliphilus) with functions of passivating heavy metal cadmium and promoting plant growth and application of the Cupriavidus alkaliphilus (Cupriavidus alkaliphilus), and relates to the technical field of microorganisms. The basophilic Cupriavidus strain KY678 is separated and screened by using a high-throughput method, and the strain has a relatively high function of passivating heavy metal cadmium, can reduce the absorption of the heavy metal cadmium by plant root systems and stem and leaf parts, and remarkably reduces the harm of the heavy metal cadmium in soil to plants; in addition, the strain also has a function of promoting plant growth, and has good popularization and application values.

The present invention provides a microorganism that synthesizes a high-molecular-weight PHA, and a method for producing a high-molecular-weight PHA, which have a productivity of at least 100 g/L. The provision is achieved by controlling the specific activity of a PHA synthase in cells of a microorganism that belongs to the genus Cupriavidus and is capable of producing a PHA, to 0.1 U/mg-protein at most. The microorganism and the method enable industrially efficient production of a PHA with a weight average molecular weight of at least 4,000,000.

The invention relates to the field of microorganisms, in particular to cupriavidus and application of the cupriavidus. The cupriavidus is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation date is September 25, 2017, and the preservation number is CCTCC M 2017546. Bacterial strain obtained by the invention can degrade 2-chloro-4-nitrophenol by taking phloroglucinol as a ring-opening substrate, and the strain provided by the invention is gram-negative bacteria capable of metabolizing 2-chloro-4-nitrophenol by a 1,2,4-benzenetriol way. On the other hand, the prepared remediation microbial inoculum can completely degrade 100 ppm of 2-chloro-4-nitrophenol in contaminated soil within 12 days. Therefore, the strain has good application prospects in the biological treatment of 2-chloro-4-nitrophenol pollution.

The invention relates to the technical field of agricultural microorganisms, and particularly discloses a microbial agent for promoting growth of oilseed rape and an oilseed rape planting method thereof. The microbial agent provided by the invention comprises the following bacteria: a) at least one of corynebacterium, paracoccus, chromobacterium, achromobacter, acidithiobacillus, acidovorax, alcaligenes, arthrobacter, bacillus, cupriavidus, derxia, herbaspirillum, hydrogenobacter, hydrogenophaga, pseudomonas, pseudonocardia, rhizobium, rhodococcus, rhodopseudomonas, rhodospirillum, thiocapsa, xanthobacter, flavobacterium and wautersia; B) acetobacter, and c) azospirillum. The microbial agent provided by the invention improves the yield of oilseed rape, and is matched with a specific oilseed rape planting method to increase the yield of oilseed rape per mu, reduce the nitrogen fertilizer demand, reduce harmful germs, restore the number of microorganisms in soil and increase the diversity of the microorganisms.

The invention relates to a hydrolysis saccharification process of alternanthera philoxeroides. Metal substance pollutants (iron, cobalt and nickel) in alternanthera philoxeroides raw materials are absorbed by adopting magnetic force, so that subsequent mechanical abrasion and contents of metal ions in treating fluid are reduced, and a good fermentation condition is created for detoxification of selected cupriavidus metallidurans; then solid-liquid separation is realized by adopting an extrusion method, hydrolysis on lipids and protein contained in liquid-state materials is carried out by adopting an NaOH solution, and then hydrolysis treatment is further carried out by adopting trypsase, so that the hydrolysis is more completely carried out; meanwhile, extruded solids are dried and crushedand then dropped into a sulfuric acid solution to carry out acidolysis; through electrorheological fluid shear stress effect treatment, cellulase is then added and enzymatic treatment is carried out,so that the effect is good and energy consumption is low. According to the hydrolysis saccharification process of the alternanthera philoxeroides disclosed by the invention, the raw material treatment efficiency is improved to the maximum extent, and finally, saccharified hydrolytic solutions rich in nutrient substances such as glucose, amino acid, small molecule chain lipids and the like are obtained, so that the glucose content is high, and the glucose can be conveniently supplied for further fermentation, and the production efficiency is improved.

The invention provides a method for improving the electric transformation efficiency of genus cupriavidus bacteria with high GC content. The method comprises the following steps: after activating thegenus cupriavidus bacteria with high GC content, inoculating into a diluted eutrophic culture medium containing a surfactant and pectinase; after culturing for a period of time, centrifuging and collecting thalli; then re-suspending and washing with sterile de-ionized water for a plurality of times; centrifuging and collecting thallus sediment; re-suspending in a pre-cooled glycerol water solutionto prepare competent cells and carrying out electric transformation. According to the method provided by the invention, the culture medium is improved to find out that extracellular polymers of the genus cupriavidus bacteria can be effectively reduced by reducing the content of nutrient substances in the culture medium and adding a proper amount of the surfactant and pectinase, so that the electric transformation efficiency of the competent cells is greatly improved. The invention develops a stable and efficient electric transformation method aiming at the characteristics of the genus cupriavidus bacteria with high GC content, and provides powerful technical supports for deeply researching and analyzing the genus cupriavidus bacteria.

It is an object of the present invention to develop a novel vector. Preferably, the object is to develop a novel vector which can be stably retained in bacteria of the genus Ralstonia, Cupriavidus or Wautersia without any antibiotic-due selective pressure and has no transferability by conjugation. Another object is to provide a strain which can stably produce polyhydroxyalkanoate using the vector, and a method for producing a polyhydroxyalkanoate using the strain.

The present invention provides a novel recombinant vector which contains an origin of DNA replication functioning in bacteria of the genus Ralstonia, Cupriavidus or Wautersia. Particularly, the transformant, which is obtained by using a recombinant vector which contains the origin of DNA replication functioning in bacteria of the genus Ralstonia, Cupriavidus or Wautersia and contains a region for a recombinant vector stabilization (par region) can make the vector to be stably retained in bacteria, and can efficiently produce a polyhydroxyalkanoate.

The invention discloses a preparation method of sodium alginate immobilized bacterial combination for polycyclic aromatic hydrocarbon pollution remediation. According to the method, benzo[a]pyrene isselected as a representative model of polycyclic aromatic hydrocarbon, sodium alginate pellets are adopted as carriers for immobilizing eight bacteria including rhodobacter P1, flavobacterium P2, rhizobium P3, Mucilaginibacter P4, flavobacterium P5, cupriavidus P6, cupriavidus P7 and cupriavidus P8, the sodium alginate pellets containing the eight bacteria are mixed in a mass ratio of P1, P2, P3,P4, P5, P6, P7 and P8 being 1:1:1:1:1:12:12:1, and a bacterial combination is obtained. The bacterial combination is obtained with a method of first immobilization and later mixing, and a ratio of strains in the bacteria combination is guaranteed while polycyclic aromatic hydrocarbon is prevented from being introduced in a culture process; bacteria are immobilized with a sodium alginate embeddingmethod, an antagonistic effect among the strains in the bacterial combination is reduced, and the efficiency of degrading polycyclic aromatic hydrocarbon by the bacteria combination is improved.