105 results about "Rhodococcus" patented technology

The present invention belongs to the field of biomedicine, and specifically relates to an antimicrobial peptide derived from lactoferrin and its application. The antimicrobial peptide derived from lactoferrin, whose amino acid sequence is shown in any one of SEQ ID NOs: 1 to 98, has a broad-spectrum antimicrobial effect, especially significant antibacterial effect against Pseudomonas putida, Rhodococcus erythropolis, Bacillus subtilis, Malassezia furfur, Cryptococcus neoformans, and Candida albicans, and has great application value.

The invention discloses a bacterial synbiotic beneficial to growth promotion of pasture in saline-alkali soil and application of the bacterial synbiotic. The bacterial synbiotic is a combination of bacterial strains NAU-RHO, NAU-MIC2 and NAU-FLA4 with 4-guanidinobutyric acid. The bacterial strains NAU-MIC2, NAU-FLA4, NAU-RHO are respectively classified and named as microbacterium laeviniform, Flavobacterium anhuiense and Rhodococcus oxyrhenium, and the research result shows that the bacterial synbiotics can be used for improving the salt tolerance and growth promoting effects of the bacteria on the medicago sativa and promoting the high yield of the medicago sativa in the saline-alkali soil.

The invention discloses an application of rhodococcus diploides in treatment of waste water containing isophorone, the rhodococcus diploides is classified and named as Rhodococcus tectodiphilus, the strain number is GXGD-YZ-2510, and the preservation number is GDMCC No: 67304. The invention further discloses a preparation method of the rhodococcus diploides. The strain shows good growth metabolism activity and isophorone degradation efficiency in a high-salt aerobic environment. Under the conditions that the system salinity is 1 wt%, the initial isophorone concentration is 200 mg / L, and the dissolved oxygen is 3-5 mg / L, the removal rate of the strain on the isophorone reaches 78.6%. The strain not only has excellent salt stress resistance, but also can realize efficient bioconversion of isophorone under high-salt aerobic conditions, and effectively solves the technical bottleneck that isophorone in high-salt wastewater in the industries of petrochemical engineering, chemical engineering, pharmacy and the like is difficult to biochemically treat; the method has the remarkable advantages of being thorough in degradation, low in cost, environmentally friendly, high in process adaptability and the like, and a novel strain resource and an industrialization technical scheme are provided for biological treatment of high-salinity wastewater of refractory organic pollutants.

The invention relates to the technical field of microbial engineering, and particularly discloses a compound microbial agent for treating cyanobacterial bloom, bacillus velezensis provided by the invention destroys algae cells and inhibits the activity of related biological enzymes by secreting heterocyclic algal killing active substances, and simultaneously causes the leakage of cellular contents (such as algal toxins, polysaccharides and lipids); an extracellular enzyme system (such as alkaline phosphatase and beta-glucosidase) of the sphingomonas decomposes free algal toxins into low-toxicity linear peptide fragments and hydrolyzes beta-1, 4-glucosidic bonds in EPS at the same time, so that algae-derived organic fragments are converted into small-molecular soluble substances, and a substrate is provided for follow-up metabolism of rhodococcus; lipase and esterase secreted by rhodococcus can hydrolyze triglyceride and waxy components, fatty acid is converted into energy and a biosurfactant through a beta-oxidation pathway, and hydrophilicity of frond fragments is enhanced to promote overall degradation efficiency.

The invention belongs to the technical field of symbiotic colonization of microorganisms and plants, and particularly relates to a fungicide for relieving phosphorus starvation of rice as well as a preparation method and application of the fungicide, including fungus mortierella which can effectively adapt to a soil flooding environment and is slightly influenced by anaerobic environments such as water logging when symbiotic colonization occurs between the mortierella and rice; on the basis, a bacterial mixed group of rhodococcus erythropolis and pseudomonas aeruginosa is further introduced, acid-aluminum stress of rice rhizosphere can be effectively reduced, stiffness of root tip cell walls is relieved, symbiotic colonization of mortierella to rice is further promoted, abundance of mortierella in rice rhizosphere soil is improved, and rice yield is increased. Therefore, the utilization capability of the rice on phosphorus nutrients is improved.

The invention provides Rhodococcus qingshengii FJ5 capable of promoting tobacco growth and preventing and treating bacterial wilt and application of the Rhodococcus qingshengii FJ5, and belongs to the technical field of microorganisms. The invention provides a rhodococcus FJ5 strain, and the collection number of the rhodococcus FJ5 strain is CGMCC No.32631. The FJ5 strain is wide in application range, and can well grow under the condition that the temperature is 16-34 DEG C and the pH value is 5.0-8.0. The FJ5 strain promotes early growth of tobaccos and enhances stress resistance by relieving acid-aluminum stress, improving defensive enzyme activity of the tobaccos and improving secretion of growth hormones of the tobaccos and the carbon and nitrogen metabolism level of the tobaccos, and meanwhile, improves fertility of acid soil and prevents and treats bacterial wilt of the tobaccos. The Rhodococcus FJ5 provided by the invention has important development value in high-quality development of tobacco under the condition of rice-tobacco rotation acidified soil, and also has important significance in improvement of stress resistance of plants and biological control of root and stem diseases.

The invention relates to a biomarker for individual recognition and application thereof, and relates to the technical field of biology. The biomarker comprises at least one of the following microorganisms: propionibacterium acnes, staphylococcus epidermidis, marine paracoccus, streptococcus, rhodococcus fence celebrating, staphylococcus, pseudomonas, streptococcus sanguineus, achromobacter xylosoxidans, acinetobacter calcoaceticus, acinetobacter johnsonii, staphylococcus lentus, pseudomonas toralaris and brevundimonas vesicularis. Or enterococcus faecium. The biomarker can provide analysis with higher resolution in the aspect of differential strain information, has a better distinguishing effect, higher accuracy and sensitivity on individual recognition, and can be used as a new technical means for supplementing forensic individual recognition.

The application discloses a microorganism-loaded activated carbon composition, a nitrile-containing wastewater pretreatment process and device and the device, and belongs to the technical field of microorganism culture and wastewater treatment device. In view of the technical problems that the electric conduction capacity and biodegradation efficiency of biological filler are poor when an electric catalysis internal circulation aeration biological filter is used for pretreatment of nitrile-containing wastewater in the prior art, and only the biodegradability of wastewater is improved, and the organic nitrile cannot be converted into available substrate, the application provides a microorganism-loaded activated carbon composition, which comprises activated carbon loaded with Shewanella oneidensis or Rhodococcus globerulus at a mass ratio of 1:2 to 1:3, and the amount of the activated carbon loaded with Shewanella oneidensis or Rhodococcus globerulus is 10 9 ~ 10 10 CFU / g, and the composition has good electric conduction capacity and biodegradation efficiency. The application further provides a nitrile-containing wastewater pretreatment process and device, which comprises the following steps: introducing nitrile-containing wastewater into the device body, and sequentially using electric treatment and a micro-nano bubble generator for treatment, and has the advantages of low power consumption, low treatment cost, convenient and safe operation, green and environment-friendly process and the like.

The invention discloses a haloalkane dehalogenase mutant with high stereoselectivity and application thereof.The haloalkane dehalogenase mutant F131M / F168M / I246L is obtained by modifying haloalkane dehalogenase DhaA31 coded by a rhodococcus dhaA gene, the haloalkane dehalogenase mutant has high degradation efficiency on haloalkane pollutants 1, 2, 3-tribromopropane, and the haloalkane dehalogenase mutant has high stereoselectivity on haloalkane pollutants 1, 2, 3-tribromopropane under the water-phase normal-temperature condition. The degradation rate of the alkyl halide dehalogenase mutant to 10 mM of 1, 2, 3-tribromopropane within 60 min reaches 100%, and the degradation rate of the alkyl halide dehalogenase mutant to 10 mM of 1, 2, 3-tribromopropane reaches 100%. The haloalkane dehalogenase mutant has high stereoselectivity on a high-value product (S)-2, 3-dibromo-1-propanol, and the e.r. Value of the haloalkane dehalogenase mutant reaches 98: 2. The haloalkane dehalogenase mutant provided by the invention has high catalytic efficiency and high stereoselectivity, and has a good application prospect in bioremediation and recycling of emerging haloalkane pollutants.

The present invention discloses a bacterium that degrades benzene series, a biological detoxification agent, and its use. The deposit number of the bacterium LH-HF0020 is CGMCC No. 27555. The bacterium LH-HF0020 can grow normally in the NaCl range of 0% to 10%, has strong salinity tolerance, and can help degrade benzene series and COD in a 3% high-salt environment. Cr The present invention provides a simple, rational, and quick method for preparing the Rhodococcus etheriferous fermentation broth. It significantly improves COD removal and total nitrogen degradation in the biochemical system without changing the structure. The method has strong BTEX detoxification capabilities, high-salt COD removal, and total nitrogen degradation, demonstrating promising prospects for industrialization.

This invention discloses a method for coupling physicochemical treatment and biodegradation of plastics. The method includes the following steps: S1. Mixing plastics, organic solvents, and a metal catalyst, and reacting to degrade them to obtain organic products. The metal catalyst is cobalt acetate and / or manganese acetate; S2. Biodegrading the organic products from step S1 using a mixed colony, which includes *Pseudomonas*, *Bacillus*, *Flavobacterium*, and *Rhodococcus*. This invention uses cobalt acetate and / or manganese acetate as metal catalysts. The catalytic oxidation of the metal catalyst can efficiently depolymerize traditional plastics, overcoming the bottleneck of C-C bond breaking that is difficult for microorganisms to achieve. It can successfully convert plastics into organic products mainly composed of dicarboxylic acids, benzoic acid, and terephthalic acid, and the mixed colony process completely mineralizes the organic products. This method achieves complete removal of plastics without producing any toxic or harmful substances.

The invention relates to the technical field of genetic engineering and bioengineering, in particular to a Rhodococcus lipolytica engineering bacterium and application thereof in preparation of sclareol, the Rhodococcus lipolytica engineering bacterium comprises SsLPPS, SsSCS, nucleotide for encoding polypeptide interaction tags RIAD and RIDD, and the nucleotide for encoding polypeptide interaction tags RIAD and RIDD is used for encoding polypeptide interaction tags RIAD and RIDD. The Yarrowia lipolytica engineering bacterium overexpresses an MVA pathway key gene and a GGPP synthesis related gene, and the MVA pathway key gene is selected from erg8, erg10 and erg20; the GGPP synthesis related gene is selected from tPaGGPPS and / or SsGGPPS, and the GGPP synthesis related gene is selected from the group consisting of tPaGGPPS and / or The promoter of the erg9 gene of the Yarrowia lipolytica engineering bacterium is a truncated promoter, and the length of the promoter is 40-110 bp. According to the method, the sclareol is efficiently synthesized in the Yarrowia lipolytica, and the final shake flask yield reaches 1.5 g / L.

The invention discloses a raw lacquer sensitization removal method based on a rhodococcus degradation technology, which belongs to the technical field of biological desensitization, and comprises the following steps: step a, inoculating a cryopreserved strain of rhodococcus erythropolis with the strain number of CGMCC (China General Microbiological Culture Collection Center) 4.1814 into an inorganic culture medium containing beta-caryophyllene for shake culture, centrifugally collecting the thalli subjected to shake culture, and finally resuspending the collected thalli by using sterile normal saline to obtain a bacterial suspension; step b, homogenizing natural raw lacquer, subpackaging the homogenized natural raw lacquer into a sterile container, adding an inorganic culture medium and the bacterial suspension into the sterile container, and mixing by adopting a sterile magnetic stirrer to ensure that thalli are uniformly dispersed so as to obtain an inoculated raw lacquer reaction solution; and step c, putting the sterile container containing the inoculated raw lacquer reaction liquid into a constant-temperature shaking table under the condition of maintaining an aerobic environment, and carrying out shaking culture. The raw lacquer desensitizing agent can degrade various allergic components, realizes efficient desensitization, does not influence the performance of raw lacquer, and is also high in environmental protection property.

The application provides a strain of Rhodococcus ZJUHULJT1 and application of the strain in prevention and treatment of paratyphaceae root-knot nematodes, and belongs to the technical field of biological prevention and treatment of root-knot nematodes. Rhodococcus The Rhodococcus (sp.) ZJUHULJT1 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251182; the Rhodococcus ZJUHULJT1 can significantly inhibit the infection and reproduction of root-knot nematodes, reduce the number of rice root knots, promote the synthesis of defense-related hormones of rice roots, and improve the disease resistance of rice; through the multiple inhibition of paratyphaceae root-knot nematodes, the Rhodococcus ZJUHULJT1 can be applied to the prevention and treatment of rice root-knot nematodes, has good prevention and treatment effect, and provides a new technical means for the pollution-free reduction of paratyphaceae root-knot nematode diseases.

The invention belongs to the technical field of soil remediation, and particularly relates to an alpine mining area root-promoting bacterium solution and a preparation method and application thereof. The strains in the root-promoting bacterium liquid disclosed by the invention comprise sphingomonas, arthrobacter, bacillus, motility coccus and red bacillus. Results of the embodiment of the invention show that the root system and plant height of elymus dahuricus hydroponically cultured by using the root-promoting bacterium solution are obviously increased. Furthermore, the combination of the root-promoting bacterial liquid and elymus dahuricus can significantly improve the soil components of the high and cold mining area, thereby promoting the restoration of the barren soil of the high and cold mining area.

The present application relates to a method for inducing synchronous emergence of fresh corn seeds, belonging to the field of agricultural technology, wherein the fresh corn seeds are soaked in a complex microbial inoculum containing red-pink red ball bacteria, methylobacterium extorquens and argumentous bacteriophage liquid microbial inoculum at a volume ratio of 1:2-3:1-2 after disinfection and air drying, and then sowed and germinated after re-drying treatment, which can effectively induce synchronous emergence of fresh corn, improve the uniformity of emergence, and facilitate unified management and harvesting in the later planting process.

The invention discloses a genetically engineered bacterium and a method for preparing furyl organic amine by using the genetically engineered bacterium. The method comprises the following steps: carrying out catalytic reaction in a reaction system containing an amino donor by using a cell carrier for expressing transaminase; according to the catalytic reaction, aldehyde group / carbonyl group of a reaction substrate is reduced and aminated into amino group, so that furyl organic amine is prepared. According to one embodiment of the invention, synthesis of furfuryl amine is catalyzed by utilizing rhodococcus red expressing transaminase, the enzyme activity of free cells of the rhodococcus is far higher than that in escherichia coli and can reach 1.5 times, 100% conversion of a substrate can be realized, and the reaction time is less than half or more of that of the escherichia coli; the substrate tolerance of the free cells of the rhodococcus is far higher than that in escherichia coli and can reach more than 5 times; the product tolerance of the free cells of the rhodococcus is far higher than that in escherichia coli and can reach 2.5 times; compared with 2-3 times of Escherichia coli, the batch of Rhodococcus free cell reaction is increased to 7-9 times.

The invention relates to the technical field of microorganisms and environmental protection, and discloses a BTEX facultative anaerobic degrading bacterium and application thereof, the BTEX facultative anaerobic degrading bacterium is Rhodococcus sp. HB3, and the preservation number of the BTEX facultative anaerobic degrading bacterium is GDMCC No: 63282. The rhodococcus HB3 can efficiently degrade benzene, methylbenzene, dimethylbenzene and ethylbenzene, and is good in universality; the rhodococcus HB3 can rapidly grow in a micro-aerobic (2-8%) environment at 10-37 DEG C, the salt tolerance can reach 3.0%, and the rhodococcus HB3 can adapt to a low-oxygen and salinized oil sludge polluted soil environment, so that the strain has a good removal effect on benzene series in oil sludge polluted soil and has an engineering application prospect.

The application discloses a process for removing pollutants in factory breeding tail water of penaeus vannamei, and belongs to the technical field of breeding tail water treatment. The process comprises the following steps: (1) filtering breeding wastewater to remove solid matters; (2) adding composite bacteria to the filtered breeding wastewater for aeration treatment to obtain aeration wastewater, wherein the composite bacteria comprise, by weight, 1-2 parts of rhodococcus, 2-3 parts of bacillus subtilis, 3-4 parts of halococcus and 0.8-1.5 parts of photosynthetic bacteria; (3) adding a flocculating agent to the aeration wastewater, treating the aeration wastewater at 45-50 DEG C for 0.5-1 h, and filtering to obtain a first filtrate; (4) adding modified activated carbon to the first filtrate, treating the first filtrate at 45-50 DEG C for 1-2 h, filtering to obtain a second filtrate; and (5) flowing and mixing the second filtrate through a macroporous adsorption resin to obtain a third filtrate, and sterilizing the third filtrate. The application realizes efficient removal of pollutants in the breeding tail water of penaeus vannamei through the combination of physical, chemical and biological treatments.

The invention provides a restoration method based on black and odorous bottom mud in-situ chemical oxidation-biological synergy, and belongs to the technical field of environmental engineering. Comprising the following steps: adding a composite chemical oxidant into the black and odorous bottom mud to be repaired, reacting for a preset time, and adding a composite microbial agent into the same area; the complex microbial inoculant comprises microbial flora, growth factors and a composite carrier; wherein the microbial flora comprises pseudomonas putida, thiobacillus denitrificans, halomonas salina and rhodococcus erythropolis. The composite chemical oxidant provides rapid initial pollution reduction, and the composite microbial inoculant has efficient colonization ability, can perform long-term biodegradation and synergistic remediation, ensures the durability of the effect, solves the problem of mutual inhibition of chemical and biological processes, and is environment-friendly. The method is in-situ remediation, large-scale earthwork is not needed, the total cost is reduced, the method is completed mainly by adding chemicals, and the method is easy to apply and popularize in actual engineering.

The present invention discloses a highly stereoselective haloalkane dehalogenase mutant and its application, which is effective for the treatment of Rhodococcus isA The genetically encoded haloalkane dehalogenase DhaA31 was modified to obtain the haloalkane dehalogenase mutant F131M / F168M / I246L. The haloalkane dehalogenase mutant has a high degradation efficiency for the haloalkane pollutant 1,2,3-tribromopropane. Under normal temperature conditions in an aqueous phase, the haloalkane dehalogenase mutant has a degradation rate of 100% for 10 mM 1,2,3-tribromopropane within 60 minutes; the haloalkane dehalogenase mutant has high stereoselectivity for the high-value product (S)-2,3-dibromo-1-propanol, with an er value of 98:2. The haloalkane dehalogenase mutant provided by the present invention has high catalytic efficiency and high stereoselectivity, and has good application prospects in the bioremediation and resource utilization of emerging haloalkane pollutants.

The application relates to application of Rhodococcus in degradation of benzene[a]pyrene in a water body and belongs to the technical field of microbial engineering. A Rhodococcus activation strain is subcultured with LB liquid culture medium, the cell concentration is adjusted, and the strain is inoculated into liquid culture medium containing benzene[a]pyrene at a volume ratio of 5%, the final concentration of benzene[a]pyrene in the culture medium is 0.5 mM; and the sample is cultured in a shaking table under the conditions that the temperature is 27 DEG C, the pH is 7.0, and the rotating speed is 180 rpm for 5 days. The Rhodococcus can grow and metabolize by taking benzene[a]pyrene as the only carbon source and energy source, and the benzene[a]pyrene can be efficiently degraded. Through systematic optimization of the degradation conditions, the optimal process parameters are determined, the degradation efficiency is obviously improved, and the application has the advantages of low cost, no secondary pollution, high degradation efficiency and the like. The application provides an efficient and environment-friendly technical scheme for biological remediation of benzene[a]pyrene pollution in a water body, and has good ecological benefits and wide application prospect.

This invention discloses a strain that degrades various lignin-derived aromatic compounds and its application in detoxification of hydrolysate. The strain is classified as *Rhodococcus ethereans*. Rhodococcus ether-eating Strain N1, deposited at the China Center for Type Culture Collection (CCTCC) on August 11, 2022, with accession number CCTCC NO: M 20221270, can degrade p-hydroxybenzoic acid, p-coumaric acid, ferulic acid, vanillin, coniferyl alcohol, and syringaldehyde. After lignin depolymerization, derivative phenolic inhibitors are formed, mainly divided into three types: S-type (syringaldehyde), G-type (ferulic acid), and H-type (p-coumaric acid), which affect the fermentation of the strain. The Rhodococcus bacterium in this invention can efficiently degrade SGH-type monomers, achieving a removal efficiency of up to 68.4% when applied to the biological detoxification of hydrolysate inhibitors. Strain N1 has significant application value in improving the conversion efficiency of lignocellulose hydrolysate and reducing the detoxification cost in industrial production.

The present invention belongs to the fields of genetic engineering and enzyme engineering, and particularly relates to a petroleum hydrocarbon degradation gene and its application. The present invention provides a gene alkane-1-monooxygenase gene that can be used for degrading petroleum hydrocarbons alk B1_3, and the alkane-1-monooxygenase gene alk B1_3 is derived from the petroleum hydrocarbon-degrading bacterium Rhodococcus sp. RHZ01, and the alk nucleotide sequence of B1_3 is shown in SEQ IN NO.1. The present invention constructs a recombinant vector pMBP1- alk B1_3 containing the alkane-1-monooxygenase encoding gene alk B1_3, and expresses it in the prokaryotic host cell Escherichia coli BL21(DE3). Experiments prove that after the gene is expressed in the prokaryotic host cell, the obtained purified monooxygenase has the activity of catalyzing the hydrolysis of alkane substrates, and has great application potential in petroleum hydrocarbon degradation and bioremediation of contaminated soil.

The invention relates to an application of carboxylesterase TfdI in degradation of phenoxy carboxylic acid herbicides. The invention provides application of rhodococcus pyridinivorans Y6 in degrading phenoxy carboxylic acid herbicides, a novel phenoxy carboxylic acid herbicide degrading enzyme TfdI is cloned from the rhodococcus pyridinivorans Y6, the rhodococcus pyridinivorans Y6 has excellent catalytic action on phenoxy carboxylic acid herbicides, the degradation rate on 2, 4-D isooctyl ester with the concentration of 100 mg / L within 1 h is 100%, and the degradation effect is stable. The excellent degrading enzyme provided by the invention has good application potential in bioremediation of the environment polluted by the phenoxy carboxylic acid herbicide, a new scheme is provided for solving the problem of residual pollution of the 2, 4-D isooctyl ester herbicide in agricultural production, and meanwhile, a herbicide degradation gene resource library is enriched.

The embodiment of the invention discloses a rhodococcus equi antigen protein, a recombinant expression vector, a recombinant protein, a subunit vaccine and a preparation method. The coding gene sequence of the rhodococcus antigen protein VapA is as shown in SEQ ID NO: 001, and the coding gene sequence of the rhodococcus antigen protein VapG is as shown in SEQ ID NO: 002; the recombinant expression vector contains a coding gene of the rhodococcus equi antigen protein VapA or VapG; the recombinant expression vector pPICZ [alpha] A-VapA or the recombinant expression vector pPICZ [alpha] A-VapG is constructed by respectively cloning a coding gene of a rhodococcus equi antigen protein VapA or VapG into a pichia pastoris expression vector pPICZ [alpha] A; the recombinant expression vector pPICZ alpha A-VapA or pPICZ alpha A-VapG is further subjected to transformation, amplification, induced expression and purification to respectively obtain recombinant proteins VapA and VapG, and the Rhodococcus equi subunit vaccine prepared from the recombinant proteins VapA and / or VapG has a good prevention effect on Rhodococcus equi infection.

This application relates to the field of biotechnology, and particularly to primer pairs, primer-probe compositions, PCR premixes, kits, and methods for detecting multiple respiratory pathogens. Nucleic acid sequences are shown in primer pairs as indicated by SEQ ID NO: 1-2, 4-5, 7-8, 10-11, 13-14, 16-17, 19-20, 22-23, 25-26, 28-29, 31-32, 34-35, 37-38, and 40-41. For target respiratory pathogens, this application designs specific amplification primer pairs, paired with suitable probes, enabling the simultaneous detection of eight targets. Furthermore, the detection process avoids interference from heme, trimethoprim, sulfamethoxazole, amphotericin B, itraconazole, fluconazole, azithromycin, adrenaline, and lidocaine hydrochloride in the test sample. It exhibits good anti-interference properties and shows no cross-reactivity with *Rhodococcus equi*, *Candida tropicalis*, and *Candida krusei*.

The application belongs to the technical field of environmental microorganisms, and particularly relates to a strain of Rhodococcus and application thereof. Rhodococcus qingshengii The strain is Rhodococcus qingshengii (abbreviated as strain R), which has been preserved in the China General Microbiological Culture Collection Center on May 13, 2025, and the strain preservation number is CGMCC No.34527. The high-efficiency fermentation iron reduction ability of the strain under low-temperature (4 DEG C) and strict anaerobic conditions can not only be applied to the repair of contaminated sites in cold regions, especially those deep anaerobic environments with low-temperature stress, such as high-latitude groundwater, polar soil and even glacial sediment environments, but also help to understand the influence of extreme low temperature on the fermentation iron reduction process of microorganisms. This study helps to develop innovative in-situ bioremediation technology to solve a series of trivalent iron pollution problems in high-cold anaerobic environments.

The invention discloses a composite water purifying agent capable of rapidly removing COD (Chemical Oxygen Demand), and relates to the technical field of microbial water purifying agents. Comprising the following raw materials in parts by weight: 10-15 parts of bacillus subtilis, 8-12 parts of rhodococcus, 5-8 parts of sphingomonas, 3-5 parts of lactic acid bacteria, 5-10 parts of rhodopseudomonas palustris, 3-5 parts of magnetic modified nitrogen-fixing cyanobacteria, 27.5-30.5 parts of a multi-layer compound enzyme carrier and 0.1-0.2 part of trehalose. The composite water purifying agent capable of rapidly removing COD, prepared by the invention, has excellent COD removal capability, environmental adaptability, capability of treating refractory substances and ecological safety.

This invention discloses a strain of *Rhodococcus gordoniae* DLY03 and its applications. This strain was deposited on April 25, 2022, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M 2022479. The *Rhodococcus gordoniae* DLY03 provided by this invention achieved a degradation rate of 60.68% for an initial concentration of 50.0 mg / L of oxadiazon within 3 days and 82.06% within 5 days in a selective inorganic salt medium (cadmium 20 mg / L). When cultured in inorganic salt media containing different cadmium concentrations, the strain exhibited a maximum tolerance concentration of cadmium up to 100 mg / L. This indicates that *Rhodococcus gordoniae* DLY03 not only efficiently degrades oxadiazon but also possesses good cadmium tolerance, providing strong technical support for the remediation of combined oxadiazon and cadmium pollution and holding significant importance for solving complex environmental pollution problems.