335 results about "Rhodococcus" patented technology

The invention discloses a multifunctional microorganism soil repairing agent as well as preparation and application thereof and belongs to the fields of biological techniques and environment protection. The multifunctional microorganism soil repairing agent is made of the following raw materials in parts by weight through uniform mixing: 30-60 parts of nano chitosan, 10-20 parts of humic acid, 10-20 parts of nano charcoal and 20-30 parts of a composite microbial agent, wherein the composite microbial agent is prepared from fresh benzene bacillus, B.sonorensis, lysinibacillus sphaericus, bacillus licheniformis, bacillus circulans, bBacillus thuringiensis, achromobacter denitrificans, saccharothrix australiensis, Rhodococcus zopfii, pseudonocardia sulfidoxydans and pseudonocardia dioxanivorans through purification, optimization, fermentation and drying dehydration. The multifunctional microorganism soil repairing agent disclosed by the invention is capable of replacing conventional physical and chemical repairing techniques, is capable of degrading complex pollutants, is wide in repairing range, is capable of preventing soil degradation and inhibiting diseases and insects in environments, and has the advantages of being good in repairing treatment effect, low in cost and economic and environment-friendly.

The invention discloses a pyridine-degrading bacterium as well as a composite bacterial agent, a preparation method and uses thereof. The newly-separated Rhodococcus pyridinovora KT-J002 CGMCC No.2789 and Cellulomonas sp KT-J007 CGMCC No.2788 not only can effectually degrade the high concentration pyridine, but also has tolerance or degradation ability for toxic substances such as benzene, phenol, dimethylbenzene, quinoline, cyanide and the like; the composite bacterial agent composed of Rhodococcus pyridinovora KT-J002 CGMCC No.2789, Cellulomonas sp KT-J007 CGMCC No.2788, Paracoccus d enitrificans W12 CGMCC No.1673, Micrococcus luteus ATCC 49442 and Arthrobacter crystallopoietes ATCC 15481 is used for environmental improvement, can effectually degrade the pyridine and is particularly suitable for the biological treatment of coking waste water.

The present invention is directed to methods for delaying a plant development process comprising exposing a plant or plant part to one or more bacteria or enzymes. In specific embodiments, the one or more bacteria are selected from the group consisting of Rhodococcus spp., Pseudomonas chloroaphis, Brevibacterium ketoglutamicum, and a mixture comprising any combination of these bacteria. Apparatuses for delaying a plant development process comprising a catalyst that comprises one or more of the above bacteria.

The invention discloses rhodococcus ruber and application of same as an immunologic adjuvant in preparing vaccine. The rhodococcus ruber is also called rhodococcus ruber RDC-01, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO. 16640. The rhodococcus ruber disclosed by the invention has the function of increasing and regulating the body immunity andis capable of nonspecifically enhancing the activity of TB (Tuberculosis) lymphocyte, macrophagocyte and NK cells and inducing multiple cell factors such as interferon, and the rhodococcus ruber canbe used as the immunologic adjuvant after being inactivated so as to be added in an oil-adjuvant inactive vaccine, so that generation of an animal antibody induced by the vaccine can be obviously promoted; compared with single use of the oil-adjuvant inactive vaccine, a high-titre antibody can be generated, the use is safe, long-term and short-term toxic and side effects are not generated, and anapplication prospect in the field of preparation of vaccines for animals is good.

The invention relates to a biological agent for processing industrial sewage, a preparation method and application. The biological agent is prepared by the following raw materials in percentage by weight; 25% of rhodococcus, 20% of bacillus subtilis, 15% of pseudomonas aeruginosa, 10% of saccharomyces cerevisiae, 10% of nitrosobacteria, 10% of bacillus megatherium, 5% of aspergillus niger and 5% of paracoccus denitrificans. According to the biological agent provided by the invention, the strains are reasonably matched, symbiotic and coordinative and out of antagonism; after being added to a sewage treatment system, the trains are good in effect of degrading macromolecular toxic and harmful substances which are hardly degraded, such as phenols, aromatichydrocarbons, ammonia nitrogen and phosphorus; and the biological agent has unique effect of processing high-concentration organic sewage and high-ammonia nitrogen sewage which are hardly processed by conventional activated sludge process.

The invention relates to a rhodococcus ruber and a method for preparing 5-cyanovaleramide by utilizing the same. The strain needs oxygen, does not need power, spores and gram-positive bacteria, is already stored in the Common Microorganism Center of Chinese Microorganism Strain Storage Management Committee and has storage data of Jun. 4, 2009 and a preservation number of CGMCC No.3090. The method for preparing the 5-cyanovaleramide by utilizing the rhodococcus ruber comprises the following steps: (1) culturing seeds; (2) fermenting and culturing; (3) preparing a rhodococcus ruber suspension; (4) preparing a crude product of 5-cyanovaleramide; and (5) purifying the 5-cyanovaleramide. The invention provides a method for generating the 5-cyanovaleramide by selectively hydrolyzing adiponitrile in a rhodococcus ruber region, has high catalytic efficiency, high adiponitrile conversion rate up to 100 percent and 5-cyanovaleramide regioselectivity up to 100 percent, does not generate a side product of adipamide and simplifies the separation purification process of the 5-cyanovaleramide.

This invention provides ZJB-064(Rhodococcus rubber ZJB-064) of a new microbial strain selected from soil and its application in preparing p-hydroxyben acetamide, in which, said ZJB-064 was preserved in China tipical cultivated subject center in April 14, 2006 with the preservation number of CCTCCNo: M206040 and is used in sybthesizing p-hydroxyben acetamide.

The invention discloses rhodococcus ruber FQ-2 and an application thereof in degradation of acetone and other common industrial organic pollutants. The application method comprises the steps: an inorganic salt culture medium containing acetone and other common industrial pollutants is inoculated with the rhodococcus ruber FQ-2, a degradation reaction is carried out under the conditions of the temperature of 30 DEG C and the rotating speed of 160 r/min, and thus the organic pollutants are degraded; the common organic pollutants comprise acetone, n-hexane, carbon disulfide, chlorobenzene, butylacetate, ethyl acetate or alpha-pinene. The rhodococcus ruber FQ-2 is taken from activated sludge of an aeration tank of a pharmaceutical factory in Zhejiang province, has good degradation effect on VOCs organic pollutants, especially acetone, and can completely convert acetone into harmless substances such as CO2, H2O, cell biomass and the like; at the same time, the bacterial strain also can degrade common industrial pollutants such as carbon disulfide and chlorobenzene in different extent, so the bacterial strain has broad application prospects in biological purification of industrial wastegas and wastewater.

Genes have been isolated from Rhodococcus erythropolis AN12 strain encoding the isoprenoid biosynthetic pathway. The genes and gene products are the first isolated from a Rhodococcus strain. The genes and gene products of the present invention may be used in a variety of ways for the production of isoprenoid compounds in a variety of organisms.

The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.

The invention relates to the field of microorganisms, in particular to deodorant predominant bacteria, a composite microbial inoculum and application. In the invention, the strains obtained by sieving are Rhodococcus (collection center accession serial number: China General Microbiological Collection Center (CGMCC) No. 3276), Pseudomonas (collection center accession serial number: CGMCC No. 3277) and Saccharomyces (collection center accession serial number: CGMCC No. 3278). After the combination of the three strains with CODs (Chemical Oxygen Demand) of 88.1%, 81.2% and 76.5% respectively is removed, the processing effect is 90.1%; and after the combination of the three strains with TNs of 68.1%, 63.2% and 50.3% respectively is removed, the processing effect is 69.8 percent. The deodorant effect of the deodorant bacteria is remarkable, and the removal rate of organics can reach more than 65% at the same time. Compared with other processing technologies, the combined bioremediation treatment technique better accords with national policies of recycling economy, and energy saving and emission reduction. The combined bioremediation treatment technique can save a great deal of operating cost for water areas of various regions and realize the resource treatment of waste water.

The present invention provides a method for preparing a hybrid gene. The method includes a step of amplifying a P450 gene fragment contained in a sample using primers designed on the basis of regions of a plurality of P450 in which amino acid sequences are highly conserved and a step of preparing the hybrid gene using the amplified fragments and a known P450 gene. The method includes no culturing step or a step of normalizing extracted DNAs and is useful in isolating a P450 gene from various microbial resources.

The present invention further provides a fused cytochrome P450 monooxygenase containing a peptide which is linked to the C-terminus of a P450 protein with a linker portion disposed therebetween and which has the same function as that of a reductase domain contained in a cytochrome P450 monooxygenase originating from Rhodococcus sp. strain NCIMB 9784. This enables the construction of a high-efficiency electron transfer system useful for various P450 proteins and also enables the production of an active P450 monooxygenase.

The invention discloses a preparation method of a composite microbial inoculant for degrading aflatoxin B1 and a composite microbial inoculant prepared by the method. The method comprises the following steps: respectively culturing pseudomonad, Flavobacterium, Rhodococcus, Stenotrophomonas and Bacillus to obtain a pseudomonad solution, a Flavobacterium solution, a Rhodococcus solution, a Stenotrophomonas solution and a Bacillus solution; mixing the pseudomonad solution, Flavobacterium solution, Rhodococcus solution, Stenotrophomonas solution and Bacillus solution to obtain a composite bacterium solution; and putting the composite bacterium solution into a composite bacterium culture medium, and carrying out mixed culture fermentation to obtain the composite microbial inoculant for degrading aflatoxin B1. The composite microbial inoculant can efficiently degrade aflatoxin B1, and has the advantages of low cost and no secondary pollution.

The invention discloses an insecticide for tomatoes. The insecticide comprises the following components in parts by weight: 12-13 parts of pseudomonas powder, 8-10 parts of ammonium dihydrogen phosphate, 6-8 parts of deltamethrin, 13-16 parts of tetramethrin, 0.15-0.18 part of green muscardine fungus powder, 1.8-2.3 parts of veratrine, 2-2.3 parts of toosedarin, 3-3.3 parts of C30H45O5, 2-2.3 parts of saponin nicotine, 2.4-3.0 parts of veratrine, 1.9-2.3 parts of phage powder, 2.6-3.3 parts of rhodococcus powder, 12-18 parts of nicotine, 19-23 parts of tripterygium wilfordii, 15-20 parts of agrimonia pilosa, 3-5 parts of stellera chamaejasme, 7-10 parts of Chinaberry fruit, 1.2-1.6 parts of bacillus thuringiensis powder, 16-23 parts of Chinaberry bark, 19-25 parts of leech, 14-19 parts of wild chrysanthemum, and 100-200 parts of water.

The invention relates to a method for treating or preventing (including immunising against) post-weaning multisystemic wasting syndrome (PMWS) and/or porcine dermatitis and nephropathy syndrome (PDNS) in a subject comprising administering an effective amount of a pharmaceutical composition or immune modulator composition comprising a whole cell of a bacterium from one or more of the genera Rhodococcus, Gordonia, Nocardia, Dietzia, Tsukamurella and Nocardioides, to said subject. In addition the method relates to the use of an immune modulator composition or a pharmaceutical composition comprising a whole cell of a bacterium from the genera Rhodococcus, Gordonia, Nocardia, Dietzia, Tsukamurella and Nocardioides, in the manufacture of a medicament for the treatment or prevention of post-weaning multisystemic wasting syndrome (PMWS) and/or porcine dermatitis and nephropathy syndrome (PDNS).

The invention provides a mixed bactericide for restoring places polluted by polynuclear aromatic hydrocarbons, a preparation method thereof, belonging to the field of environment pollution restoration engineering. The strains used by the bactericide are Bordetella, Ochrobactrum, Rhodococcus, Achcromobacter, Herbaspirillum and Microbacterium which are mixed according to the ratio of 21-101:40-200:22-102:20-100:1:2 for fermentation to obtain a bacterial liquid, and the bacterial liquid is mixed with solid carriers according to the mass ratio of 0.5-1:1 to obtain a solid bactericide which has the effective living bacteria count of 108-1011/g. The mixed bactericide in the invention can effectively remove polynuclear aromatic hydrocarbons polluting soils and water, and has the advantages of high activity, rapid growth and propagation, strong adaptability, no generation of secondary pollution and the like.

The invention belongs to the field of wastewater treatment and discloses a treatment process applicable to industrial wastewater. The treatment process applicable to the industrial wastewater comprises the following steps: 1) preparing complex microbial inoculant; 2) precipitating wastewater; 3) purifying the wastewater; 4) preparing bacterial manure. The prepared complex microbial inoculant comprises 5-6 parts of micrococcus luteus, 4-5 parts of bacillus mucilaginosus, 3-4 parts of paracoccus denitrificans, 2-3 parts of rhodococcus, 2-3 parts of acidithiobacillus ferrooxidans, 1-2 parts of bacillus megaterium and 1-2 parts of bacillus cereus. The invention also discloses bacterial manure prepared by utilizing the treatment process applicable to the industrial wastewater. The treatment process applicable to the industrial wastewater is simple and feasible, the industrial wastewater can be effectively treated, and the bacterial manure is prepared by utilizing the treatment process applicable to the industrial wastewater.

The invention discloses rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof. The invention provides a strain namely rhodococcus ruber YMHL-1 capable of degrading residual nicosulfuron in wastewater, and the identification shows that the strain belongs to Rhodococcus. The strain is preserved in China General Microbiological Culture Collection Center, CGMCC in February 9th, 2015; and the preservation number is CGMCC No. 10542. The main biological characteristics are as follows: the Gram staining reaction is negative; the bacterial colony is in an orange yellow color, is in a round shape, and is opaque, the surface of the bacterial colony is rough and dry; the cell is in a sphere shape or short bar shape, does not have any flagellum, is motionless, and does not generate any spore; the strain is aerobic and chemoheterotrophic and cannot liquefy gelatin and hydrolyze starch; the enzyme contact reaction is positive, the V.P. reaction is positive, the methyl red reaction is negative; the strain cannot degrade casein, cannot reduce nitrates; and the strain can produce acids from glucose, can utilize glucose, fructose, and citrates, cannot utilize mannose, arabinose, and raffinose, and can reduce more than 90% of antibiotics in water through direct application.

The invention relates to a special rhodococcus ruber (CGMCC NO. 17012) fermentation method, a preparation process of adjuvants with different dosage forms and an application as an adjuvant in animalvaccines. Rhodococcus ruber strains are separated from a farm and obtained through single colony cloning purification and identification, the invention provides a special fermentation process where the strain inactivation product is used as an animal vaccine adjuvant, and provides a preparation process of different dosage forms of adjuvants containing the strain and the product. Animal experimentsprove that when the adjuvant product is used for univalent or multivalent animal vaccines, particularly Newcastle disease inactivated vaccine, avian influenza inactivated vaccine and swine fever livevaccine, definite non-specific immune enhancement effect can be provided, specifically, the antibody peak value level induced by the animal vaccine is obviously improved, the time for producing the protective antibody level is advanced, the antibody maintenance period is prolonged, and the immune effect of the animal vaccines is enhanced.

The invention provides a compound microorganism preparation. The compound microorganism preparation is composed of Bacillus cereus, bacillus licheniformis, halophilic pseudomonades, Lactobacillus acidophilus, Rhodococcus erythropolis, Rhodopseudomonas palustris and enterococcus faecium. The viable count of the compound microorganism preparation is as high as 8*1011CFU/g, and the compound microorganism preparation can be widely applied to the aquaculture water areas of fresh water and seawater. Ammonia nitrogen and nitrite in the water can be effectively degraded and water blooms can be restrained under the synergistic effect of the bacterial strains, therefore, the aquatic water can be purified, growing environment for breeding animals can be improved, and productivity can be improved.

The present invention provides novel compostions and methods of using the same for genetic manipulation of a variety of bacterial strains such as Rhodococcus.

The invention relates to a preparation for trinitrotoluene-contained sewage treatment and a using method of the preparation. The preparation is formed of raw materials, by weight, 20% of nitrosobacteria, 20% of bacillus subtilis, 15% of alcaligenes faecalis, 10% of pseudomonas aeruginosa, 10% of brewer's yeast, 10% of rhodococcus fascians, 10% of bacillus megatherium and 5% of aspergillus niger. Various raw materials of the preparation are reasonably prepared and symbiotically coordinated without antagonism, and accordingly, the preparation is applicable to industrial sewage treatment, treatment quality and treatment water quality can be improved, running cost can be reduced and discharge up to standards is promoted.

The invention relates to a method for preparing biochemical preparations for treating threonine fermentation wastewater. The method includes preparing physical adsorbents and complex microbial inoculants. The complex microbial inoculants comprise, by volume, 6-7 parts of yeast, 5-6 parts of arthrobacter, 5-6 parts of pseudomonas, 4-5 parts of rhodococcus, 4-5 parts of bacillus amyloliquefaciens, 4-5 parts of clostridium and 3-4 parts of scenedesmus obliquus. The method has the advantages that the biochemical preparations contain diversified microorganisms with excellent degradation capacity for refractory pollutants, various strains are reasonably compatible with one another, accordingly, excellent degradation effects can be realized, and the method has a broad application prospect.

The invention discloses Rhodococcussp.CCZU10-1 and a method for synthesizing benzoyl formic acid through nitrile hydrolysis by using the same, and belongs to the technical field of microbes. The Rhodococcussp.CCZU10-1 has the collection number of CGMCC (China General Microbiological Culture Collection Center) No.4911. The method comprises the following steps of: performing amplification culture on benzoyl carbonitrile serving as an initiative raw material in a fermentation medium to obtain Rhodococcussp.CCZU10-1 cells; and performing hydrolysis on the benzoyl carbonitrile under certain conditions by using the Rhodococcussp.CCZU10-1 cells as a biocatalyst to obtain the product of benzoyl formic acid. The method has the characteristics of simple process route, mild reaction conditions, no pollution and the like.