52 results about "Rhodococcus" patented technology

The invention discloses an application of rhodococcus diploides in treatment of waste water containing isophorone, the rhodococcus diploides is classified and named as Rhodococcus tectodiphilus, the strain number is GXGD-YZ-2510, and the preservation number is GDMCC No: 67304. The invention further discloses a preparation method of the rhodococcus diploides. The strain shows good growth metabolism activity and isophorone degradation efficiency in a high-salt aerobic environment. Under the conditions that the system salinity is 1 wt%, the initial isophorone concentration is 200 mg / L, and the dissolved oxygen is 3-5 mg / L, the removal rate of the strain on the isophorone reaches 78.6%. The strain not only has excellent salt stress resistance, but also can realize efficient bioconversion of isophorone under high-salt aerobic conditions, and effectively solves the technical bottleneck that isophorone in high-salt wastewater in the industries of petrochemical engineering, chemical engineering, pharmacy and the like is difficult to biochemically treat; the method has the remarkable advantages of being thorough in degradation, low in cost, environmentally friendly, high in process adaptability and the like, and a novel strain resource and an industrialization technical scheme are provided for biological treatment of high-salinity wastewater of refractory organic pollutants.

The application discloses a microorganism-loaded activated carbon composition, a nitrile-containing wastewater pretreatment process and device and the device, and belongs to the technical field of microorganism culture and wastewater treatment device. In view of the technical problems that the electric conduction capacity and biodegradation efficiency of biological filler are poor when an electric catalysis internal circulation aeration biological filter is used for pretreatment of nitrile-containing wastewater in the prior art, and only the biodegradability of wastewater is improved, and the organic nitrile cannot be converted into available substrate, the application provides a microorganism-loaded activated carbon composition, which comprises activated carbon loaded with Shewanella oneidensis or Rhodococcus globerulus at a mass ratio of 1:2 to 1:3, and the amount of the activated carbon loaded with Shewanella oneidensis or Rhodococcus globerulus is 10 9 ~ 10 10 CFU / g, and the composition has good electric conduction capacity and biodegradation efficiency. The application further provides a nitrile-containing wastewater pretreatment process and device, which comprises the following steps: introducing nitrile-containing wastewater into the device body, and sequentially using electric treatment and a micro-nano bubble generator for treatment, and has the advantages of low power consumption, low treatment cost, convenient and safe operation, green and environment-friendly process and the like.

This invention discloses a method for coupling physicochemical treatment and biodegradation of plastics. The method includes the following steps: S1. Mixing plastics, organic solvents, and a metal catalyst, and reacting to degrade them to obtain organic products. The metal catalyst is cobalt acetate and / or manganese acetate; S2. Biodegrading the organic products from step S1 using a mixed colony, which includes *Pseudomonas*, *Bacillus*, *Flavobacterium*, and *Rhodococcus*. This invention uses cobalt acetate and / or manganese acetate as metal catalysts. The catalytic oxidation of the metal catalyst can efficiently depolymerize traditional plastics, overcoming the bottleneck of C-C bond breaking that is difficult for microorganisms to achieve. It can successfully convert plastics into organic products mainly composed of dicarboxylic acids, benzoic acid, and terephthalic acid, and the mixed colony process completely mineralizes the organic products. This method achieves complete removal of plastics without producing any toxic or harmful substances.

The application provides a strain of Rhodococcus ZJUHULJT1 and application of the strain in prevention and treatment of paratyphaceae root-knot nematodes, and belongs to the technical field of biological prevention and treatment of root-knot nematodes. Rhodococcus The Rhodococcus (sp.) ZJUHULJT1 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251182; the Rhodococcus ZJUHULJT1 can significantly inhibit the infection and reproduction of root-knot nematodes, reduce the number of rice root knots, promote the synthesis of defense-related hormones of rice roots, and improve the disease resistance of rice; through the multiple inhibition of paratyphaceae root-knot nematodes, the Rhodococcus ZJUHULJT1 can be applied to the prevention and treatment of rice root-knot nematodes, has good prevention and treatment effect, and provides a new technical means for the pollution-free reduction of paratyphaceae root-knot nematode diseases.

The invention discloses a genetically engineered bacterium and a method for preparing furyl organic amine by using the genetically engineered bacterium. The method comprises the following steps: carrying out catalytic reaction in a reaction system containing an amino donor by using a cell carrier for expressing transaminase; according to the catalytic reaction, aldehyde group / carbonyl group of a reaction substrate is reduced and aminated into amino group, so that furyl organic amine is prepared. According to one embodiment of the invention, synthesis of furfuryl amine is catalyzed by utilizing rhodococcus red expressing transaminase, the enzyme activity of free cells of the rhodococcus is far higher than that in escherichia coli and can reach 1.5 times, 100% conversion of a substrate can be realized, and the reaction time is less than half or more of that of the escherichia coli; the substrate tolerance of the free cells of the rhodococcus is far higher than that in escherichia coli and can reach more than 5 times; the product tolerance of the free cells of the rhodococcus is far higher than that in escherichia coli and can reach 2.5 times; compared with 2-3 times of Escherichia coli, the batch of Rhodococcus free cell reaction is increased to 7-9 times.

The application discloses a process for removing pollutants in factory breeding tail water of penaeus vannamei, and belongs to the technical field of breeding tail water treatment. The process comprises the following steps: (1) filtering breeding wastewater to remove solid matters; (2) adding composite bacteria to the filtered breeding wastewater for aeration treatment to obtain aeration wastewater, wherein the composite bacteria comprise, by weight, 1-2 parts of rhodococcus, 2-3 parts of bacillus subtilis, 3-4 parts of halococcus and 0.8-1.5 parts of photosynthetic bacteria; (3) adding a flocculating agent to the aeration wastewater, treating the aeration wastewater at 45-50 DEG C for 0.5-1 h, and filtering to obtain a first filtrate; (4) adding modified activated carbon to the first filtrate, treating the first filtrate at 45-50 DEG C for 1-2 h, filtering to obtain a second filtrate; and (5) flowing and mixing the second filtrate through a macroporous adsorption resin to obtain a third filtrate, and sterilizing the third filtrate. The application realizes efficient removal of pollutants in the breeding tail water of penaeus vannamei through the combination of physical, chemical and biological treatments.

The invention provides a restoration method based on black and odorous bottom mud in-situ chemical oxidation-biological synergy, and belongs to the technical field of environmental engineering. Comprising the following steps: adding a composite chemical oxidant into the black and odorous bottom mud to be repaired, reacting for a preset time, and adding a composite microbial agent into the same area; the complex microbial inoculant comprises microbial flora, growth factors and a composite carrier; wherein the microbial flora comprises pseudomonas putida, thiobacillus denitrificans, halomonas salina and rhodococcus erythropolis. The composite chemical oxidant provides rapid initial pollution reduction, and the composite microbial inoculant has efficient colonization ability, can perform long-term biodegradation and synergistic remediation, ensures the durability of the effect, solves the problem of mutual inhibition of chemical and biological processes, and is environment-friendly. The method is in-situ remediation, large-scale earthwork is not needed, the total cost is reduced, the method is completed mainly by adding chemicals, and the method is easy to apply and popularize in actual engineering.

The application relates to application of Rhodococcus in degradation of benzene[a]pyrene in a water body and belongs to the technical field of microbial engineering. A Rhodococcus activation strain is subcultured with LB liquid culture medium, the cell concentration is adjusted, and the strain is inoculated into liquid culture medium containing benzene[a]pyrene at a volume ratio of 5%, the final concentration of benzene[a]pyrene in the culture medium is 0.5 mM; and the sample is cultured in a shaking table under the conditions that the temperature is 27 DEG C, the pH is 7.0, and the rotating speed is 180 rpm for 5 days. The Rhodococcus can grow and metabolize by taking benzene[a]pyrene as the only carbon source and energy source, and the benzene[a]pyrene can be efficiently degraded. Through systematic optimization of the degradation conditions, the optimal process parameters are determined, the degradation efficiency is obviously improved, and the application has the advantages of low cost, no secondary pollution, high degradation efficiency and the like. The application provides an efficient and environment-friendly technical scheme for biological remediation of benzene[a]pyrene pollution in a water body, and has good ecological benefits and wide application prospect.

This application relates to the field of biotechnology, and particularly to primer pairs, primer-probe compositions, PCR premixes, kits, and methods for detecting multiple respiratory pathogens. Nucleic acid sequences are shown in primer pairs as indicated by SEQ ID NO: 1-2, 4-5, 7-8, 10-11, 13-14, 16-17, 19-20, 22-23, 25-26, 28-29, 31-32, 34-35, 37-38, and 40-41. For target respiratory pathogens, this application designs specific amplification primer pairs, paired with suitable probes, enabling the simultaneous detection of eight targets. Furthermore, the detection process avoids interference from heme, trimethoprim, sulfamethoxazole, amphotericin B, itraconazole, fluconazole, azithromycin, adrenaline, and lidocaine hydrochloride in the test sample. It exhibits good anti-interference properties and shows no cross-reactivity with *Rhodococcus equi*, *Candida tropicalis*, and *Candida krusei*.

The application belongs to the technical field of environmental microorganisms, and particularly relates to a strain of Rhodococcus and application thereof. Rhodococcus qingshengii The strain is Rhodococcus qingshengii (abbreviated as strain R), which has been preserved in the China General Microbiological Culture Collection Center on May 13, 2025, and the strain preservation number is CGMCC No.34527. The high-efficiency fermentation iron reduction ability of the strain under low-temperature (4 DEG C) and strict anaerobic conditions can not only be applied to the repair of contaminated sites in cold regions, especially those deep anaerobic environments with low-temperature stress, such as high-latitude groundwater, polar soil and even glacial sediment environments, but also help to understand the influence of extreme low temperature on the fermentation iron reduction process of microorganisms. This study helps to develop innovative in-situ bioremediation technology to solve a series of trivalent iron pollution problems in high-cold anaerobic environments.

This invention discloses a microbial agent for regulating the microecology of aquatic bodies and its preparation method. The method includes: inoculating Rhodococcus erythropolis into a liquid culture medium, activating and expanding the culture at 28°C to obtain a Rhodococcus erythropolis fermentation broth with an effective viable count of 2.0×10⁹–2.8×10⁹ CFU / mL; then subjecting the broth to stepwise low-temperature adaptation at 15°C, 10°C, and 5°C to obtain a low-temperature adapted Rhodococcus erythropolis bacterial solution; culturing Bacillus glacialis and Bacillus belesii at 37°C and mixing them at a viable count ratio of 1:1 to obtain a Bacillus bacterial solution; subsequently, compounding the low-temperature adapted Rhodococcus erythropolis bacterial solution and the Bacillus bacterial solution at a viable count ratio of 1:(0.5–4), adding survival protection components and dispersion stabilizing components, and mixing thoroughly to obtain the microbial agent.

The invention relates to the technical field of wastewater treatment, and particularly discloses a deep treatment method for coking wastewater. The invention discloses a deep treatment method for coking wastewater. The deep treatment method comprises the following steps: pretreatment, catalytic ozonation, buffer regulation, biological activated carbon treatment and sand filtration. In catalytic ozonation, the catalyst is prepared by fixing Cu-Mn composite metal on the surface of a gamma-Al2O3 ceramic ball porous carrier through a dipping-calcining method; the molar ratio of Cu to Mn in the Cu-Mn composite metal is 1: (1-4); the biological activated carbon is obtained by dipping activated carbon in a microbial bacteria solution for reaction; the microbial liquid consists of flavobacterium with the concentration of 1 * 10 < 7 >-5 * 10 < 7 > CFU / mL and rhodococcus with the concentration of 5 * 10 < 8 >-3 * 10 < 9 > CFU / mL. According to the technical scheme, the coking wastewater is treated, and the removal rate of COD, ammonia nitrogen, volatile phenol and suspended solids in the coking wastewater is effectively increased.

The invention belongs to the technical field of microbial agents for soil improvement, and particularly relates to application of a microbial agent for promoting carbon immobilization of a red soil rice field. The microbial inoculum is rhodococcus erythropolis or a synthetic microbial inoculum of rhodococcus erythropolis and pseudomonas aeruginosa, and after the rice seedlings are transplanted to red soil for 3 days, 25mL of suspension liquid of the related microbial inoculum is inoculated to the rhizosphere of each rice seedling. According to the application, the rhizosphere microenvironment is improved, input of plant photosynthetic carbon into soil is activated, meanwhile, the carbon utilization efficiency and conversion capacity of soil in-situ microbial communities are remarkably improved, accumulation of microbial source carbon is promoted, the molecular composition structure of soluble organic matter is remodeled, and finally formation and accumulation of a soil carbon library are promoted and stabilized.

The application relates to a method for bioconversion of naringenin to produce apigenin and dihydroquercetin, belonging to the field of biotechnology and food engineering, and comprising the following steps: (1) screening and mutagenic breeding of high-yield strains; (2) high-density fermentation for converting naringenin into apigenin and dihydroquercetin; and (3) extraction and refining to obtain high-purity apigenin and dihydroquercetin. Rhodococcus opacus OMK-104 bioconversion of cheap and easily available naringenin into high-value apigenin and dihydroquercetin has high conversion rate and high titer of apigenin and dihydroquercetin, and has a positive effect on reducing the production cost of apigenin and dihydroquercetin and promoting large-scale commercial application of apigenin and dihydroquercetin.

The invention provides an engineering bacterium capable of efficiently degrading aflatoxin B1 as well as a construction method and application of the engineering bacterium. The engineering bacterium PD630:: lac103sp is obtained by taking rhodococcus turbid PD630 as an original strain and expressing a modified fmb-rL103 protein coding gene, and the nucleotide sequence of the modified fmb-rL103 protein coding gene is as shown in SEQ ID NO: 1. Compared with wild rhodococcus turbid PD630, the engineering bacterium PD630:: lac103sp provided by the invention has the advantages that the degradation efficiency of the engineering bacterium PD630:: lac103sp on aflatoxin B1 is obviously improved, the problems of insufficient degradation efficiency and poor application stability of an existing strain on aflatoxin B1 are solved, and a novel functional strain and a technical support are provided for pollution abatement of aflatoxin B1.

This invention provides a method for promoting tobacco growth and preventing bacterial wilt. Rhodococcus qingshengii This invention relates to the field of microbial technology, specifically to Rhodococcus faecalis strain FJ5, with the preservation number CGMCC No. 32631. The FJ5 strain exhibits a wide adaptability range, growing well within a temperature range of 16–34°C and a pH range of 5.0–8.0. The FJ5 strain promotes early growth and enhances stress resistance in tobacco by mitigating aluminum stress, increasing the activity of tobacco defense enzymes, improving tobacco growth hormone secretion and carbon and nitrogen metabolism, while simultaneously improving acidic soil fertility and controlling tobacco bacterial wilt. The Rhodococcus faecalis FJ5 provided by this invention has significant development value for high-quality tobacco development under acidified soil conditions in rice-tobacco rotation, and is also important for improving plant stress resistance and the biological control of root and stem diseases.

The invention discloses preparation and application of an ecological and efficient chlorogenic acid-producing composite microbial inoculant. The invention provides a combined bacterium or a complex microbial inoculant containing the combined bacterium. The combined bacterium is prepared from Sicirculus novus Q7228 (CGMCC (China General Microbiological Culture Collection Center) No.35444) and Penicillium cutaneum YY2521 (CGMCC No.42146), and the complex microbial inoculant is prepared from Sicirculus novus, Penicillium cutaneum, Sicirculus novus, Sicirculus novus, Sicirculus novus, Sicirculus novus, Sicirculus novus, Sicirculus novus and Penicillium cutaneum. The novel Rhodococcus sicca Q7228 provided by the invention is a novel species. Microbial fermentation screening experiments confirm that chlorogenic acid can be produced through respective single-bacterium fermentation of the penicillium pecocinum YY2521 and the new drying rhodococcus Q7228, and the yield of green source acid can be obviously increased through co-fermentation of the penicillium pecocinum YY2521 and the new drying rhodococcus Q7228. The method can realize ecological and efficient production of chlorogenic acid.

The present disclosure relates to the technical field of biological treatment of exhaust gases, in particular to preparation of a complex microbial inoculant, an apparatus for preparing the complex microbial inoculant and application thereof. The preparation of the complex microbial inoculant is to select kitchen wastes, wood chips and EM fungus chaff as aerobic composting raw materials, and add contaminant degrading bacteria Pseudomonas mendocin NX-1, Stenotrophomonas sp. HY-2, Rhodococcus sp. YZ-1, Ralstonia sp. XZW-1, and Pseudomonas oleovorans DT4 during the aerobic composting process for joint culture until a compost pile becomes thoroughly decomposed. The complex microbial inoculant provided by the present disclosure can simultaneously degrade n-hexane, pentane, chlorobenzene and tetrahydrofuran, and has the remarkable advantages of simple preparation process, low cost, low carbon, environmental friendliness and the like. The formed microbial inoculant that is rich in efficient degrading bacteria does not need to adjust pH, has the advantages of short starting cycle, and stable and efficient exhaust gas purification, has stronger environmental adaptability, and lays a foundation for engineering application of purifying waste water and exhaust gases containing n-hexane, pentane, chlorobenzene and tetrahydrofuran by a biological method.

The invention relates to the technical field of environmental microbiological technology and agricultural bioremediation, and discloses a rhodococcus bacterium X-20 and application thereof. The Rhodococcus sp. X-20 can efficiently degrade various pesticides in liquid and soil environments, including carbendazim, dinotefuran, atrazine, pendimethalin and cyhalothrin, and especially shows specific efficient degradation ability to carbendazim. In addition, the strain has various plant probiotic functions: the strain can secrete indole-3-acetic acid (IAA), generate iron carriers, dissolve inorganic phosphorus and antagonize phytophthora nicotianae, and the growth of tobacco seedlings can be remarkably promoted. Field experiments show that the strain can relieve growth inhibition of compound pesticide pollution on tobacco, and the strain can be used for preparing microbial agents and applied to bioremediation of pesticide-polluted soil and green agriculture.

The invention provides a blood microorganism-based autism prediction model and application thereof, the prediction model takes blood microorganisms as markers, and the detection rate or relative abundance of the blood microorganisms is reduced; the risk that the blood sample suffers from autism is predicted by increasing the detection rate of blood microorganisms II or enhancing the relative abundance; the first blood microorganisms comprise ochrobactrum, pseudoalteromonas sp.3J6, rhodococcus equi, parabacteroides dieldrii, trichoderma harzianum, enterobacter hormaechei, wolbachia, yersinia enterocolitica, treponema pallidum and Elizabeth mosquito-repellent bacteria; the blood microorganisms II comprise Klebsiella compacticola and bdellovibrio bacteriovorus. According to the method, the model is constructed based on the blood metagenome data of 1946 standard autism four families, the advantages of blood microorganisms are brought into full play, disease signals are sensitively and accurately captured, the method can be applied to clinical autism prediction, and early risk assessment and layering of diseases are achieved.

This invention relates to the field of biomedical detection technology, specifically to plasma circulating microbial biomarkers for early screening of gastric cancer and their applications. The plasma circulating microbial biomarkers include: *Ralstonia solanacearum*, *Staphylococcus aureus*, *Alternaria metachromatica*, *Porphyromonas gingivalis*, *Lactobacillus reuteri*, *Rhodococcus erythropolis*, *Akkermansia muciniphila*, *Lactobacillus gasseri*, *Bifidobacterium bifidum*, and *Neisseria gonorrhoeae*. This invention screens and, for the first time, combines 10 specific microbial biomarkers with Low Pass technology. WGS technology can achieve detection results at low depths, significantly reducing screening costs while ensuring detection sensitivity, and providing a brand-new solution for early gastric cancer screening.

This invention discloses the application of *Rhodococcus etherealis* in the conversion of cholesterol to 4-androstenedione. Experiments have demonstrated that *Rhodococcus etherealis* has a highly efficient function in converting cholesterol to 4-androstenedione, and the product is easy to extract and separate, with high purity in the final product. The yield of 4-androstenedione was found to be above 0.62 mg / L. The method for obtaining 4-androstenedione is simple, environmentally friendly, and inexpensive.

The invention provides Rhodococcus qingsheng and application thereof in relieving continuous cropping obstacles of crops, and belongs to the technical field of microorganisms. The Rhodococcus qingsheng JA11 is separated and screened from the nature for the first time, phenolic acid autotoxic substances generated by ginseng continuous cropping obstacles can be efficiently degraded, the degradation rate of benzoic acid can reach 91.51%, various phenolic acids such as vanillic acid and cinnamic acid can be effectively degraded, and the degradation spectrum is wide; when the Rhodococcus qingsheng JA11 is applied under field conditions, the content of benzoic acid in soil for continuous cropping of ginseng can be effectively reduced, meanwhile, the root diseases of ginseng can be controlled to a certain extent, the growth indexes such as the survival rate and the root length of ginseng are remarkably improved, and a certain yield increasing effect is achieved.

The application discloses application of a broad-spectrum type III esterase derived from Rhodococcus in phthalate ester pollution bioremediation, and overcomes the limitation of low degradation efficiency of a natural strain; through genetic engineering technology, the esterase 5359 gene is cloned into a Rhodococcus-E.coli shuttle vector pNV18 to construct a recombinant Rhodococcus engineering bacterium WT-pNV18-5359 overexpressing the esterase. Experimental results show that the engineering bacterium significantly improves the degradation performance: in single PAEs degradation test, the degradation rates of long-chain PAEs such as DEHP, DnOP and DiNP are respectively increased by 15.71%, 13.99% and 12.82% compared with the wild type; in a mixed PAEs system, the degradation of long-chain PAEs is obviously superior, and the engineering bacterium has good genetic stability and does not affect growth. Through genetic engineering to strengthen the application of known enzymes, efficient and stable PAEs pollution bioremediation is realized.

The invention discloses a petroleum hydrocarbon degrading bacterium and microalgae co-culture system, a construction method and application, and belongs to the technical field of marine environment bioremediation, the system is formed by mixing and embedding alcanivorans, rhodococcus, dunaliella salina and chlorella in a dry weight ratio of 3: 1 in sodium alginate gel microspheres, photosynthetic oxygen supply of microalgae meets the aerobic degradation requirement, and the photosynthetic oxygen supply of microalgae meets the aerobic degradation requirement. COs generated by metabolism of degrading bacteria are used for carbon sequestration of microalgae, self-sufficiency metabolic circulation is formed, bacteria and algae space layering and day and night metabolism complementation are achieved through gel microspheres, the 21-day degradation rate of the co-culture system to diesel oil reaches 82% or above and is increased by 35% compared with single bacteria treatment, exogenous nutritive salt and aeration are not needed, and the co-culture system is suitable for in-situ remediation of marine oil spill.

A method for producing eriodictyol and dihydroquercetin by biotransformation of naringenin belongs to the field of biotechnology and food engineering, and comprises the following steps: (1) screening and mutation breeding of high-yield strains; (2) converting naringenin into eriodictyol and dihydroquercetin through high-density fermentation; and (3) extracting and refining to obtain high-purity eriodictyol and dihydroquercetin. According to the method disclosed by the invention, the Rhodococcus opacus OMK-104 subjected to multiple rounds of ARTP mutation breeding is used for biologically converting the naringenin which is low in price and easy to obtain into the eriodictyol and the dihydroquercetin with high additional value, the conversion rate is high, the titers of the eriodictyol and the dihydroquercetin are high, and the method has a good application prospect in reducing the production cost of the eriodictyol and the dihydroquercetin. And the method plays a positive role in promoting large-scale commercial application of eriodictyol and dihydroquercetin.

The invention belongs to the technical field of aquatic micro-ecological preparations, and particularly relates to application of rhodococcus coryneform CGMCC No.23799 in improvement of COD (Chemical Oxygen Demand) of a water body and denitrification. The rhodococcus coryneform HQ40 disclosed by the invention can be used for efficiently degrading organic pollutants in a low-oxygen environment and synchronously realizing total nitrogen removal; the adaptability to 0%-3% salinity (NaCl) is high, and the growth and COD degradation performance is stable; the compound has the characteristics of oxidizing sulfides, relieving bottom mud acidification and not changing the pH value of the water body, is safe and non-toxic to cultured animals, and is suitable for repairing the cultured water body.

The invention belongs to the technical field of biology, and particularly relates to a metolachlor and chloroacetamide herbicide degrading bacterium, an enzyme and application. According to the present invention, the degrading bacterium Rhodococcus sp. L-9 capable of efficiently degrading metolachlor and other chloroacetamide herbicides is found through screening, and the degrading bacterium Rhodococcus sp. L-9 can be used for efficiently degrading metolachlor and other chloroacetamide herbicides. Furthermore, according to the invention, a gene cluster metABD for degrading the metolachlor and other chloroacetamide herbicides is cloned from a strain L-9, and the gene cluster metABD can encode three-component cytochrome P450 oxidase MetABD to catalyze the degradation of the metolachlor and other five chloroacetamide herbicides (alachlor, acetochlor, propisochlor, pretilachlor and butachlor); and enantiomer selectivity is achieved in catalysis of chiral herbicide metolachlor, and good social and economic benefits are achieved.

This invention discloses a PAEs transporter system from Rhodococcus and its application in enhancing phthalate degradation. The system comprises four transporters: 0620 (amino acid sequence as shown in SEQ ID NO:1), 3572 (amino acid sequence as shown in SEQ ID NO:2), 4497 ​​(amino acid sequence as shown in SEQ ID NO:3), and 5299 (amino acid sequence as shown in SEQ ID NO:4). These transporters work synergistically and exhibit differentiated PAEs substrate preferences. The transporters provided by this invention have a broad substrate spectrum and high efficiency, offering core components and application strategies for constructing highly efficient PAEs bioremediation engineered bacteria.

The application discloses a strain of thermophilic Rhodococcus RA1 capable of efficiently degrading phenolic acid and application of the strain in preparation of a crop biocontrol microbial agent. The technical solution of the application is characterized by the following: the strain of thermophilic Rhodococcus RA1 capable of efficiently degrading phenolic acid is preserved in the China General Microbiological Culture Collection Center on December 26, 2022, and has a preservation number of CGMCC No. 26299; the phenolic acid is single phenolic acid or mixed phenolic acid; the application further specifically discloses a separation and purification method of the thermophilic Rhodococcus and application of the thermophilic Rhodococcus in the crop biocontrol microbial agent. The thermophilic Rhodococcus RA1 can efficiently degrade single phenolic acid or mixed phenolic acid with a concentration of up to 1200 mg / L, thereby relieving direct stress of the phenolic acid on plant growth, and can inhibit growth of plant pathogenic fungi, thereby preventing the plant pathogenic fungi from invading the plants, and provides a new strain resource for development of the crop biocontrol microbial agent.