273 results about "Bioconversion" patented technology

The invention relates to the technical field of biological catalysis, and discloses an M88V mutant enzyme for preparing rebaudioside I. The M88V mutant enzyme is obtained by the following mutations generated by UGT76G1: M88V: methionine of the 88th amino acid sequence of UGT76G1 is mutated into valine. Aiming at the technical bottlenecks of low RI yield and poor selectivity in the existing RA biotransformation process, an efficient solution is provided for the UGT76G1 mutant developed by the invention. The mutant can specifically catalyze conversion from RA to RI, and the conversion rate of a target product is remarkably increased to 65% or above from general less than 7%. The excellent catalytic performance of the catalyst is verified in milliliter-level to upgraded reaction systems, the key problems of conversion efficiency and process amplification are solved, and a foundation is laid for large-scale green manufacturing of a high-added-value product RI.

The invention relates to the technical field of biomass waste reutilization and soil improvement, in particular to a biological polypeptide modified activated carbon soil conditioner and a preparation method thereof. The fertilizer comprises the following components in parts by weight: 5-7 parts of low-value animal waste protein, 5-7 parts of activated carbon, 6-8 parts of a landscaping substrate, 4-8 parts of vermiculite, 2-4 parts of a compound microbial agent and 4-8 parts of chitosan, low-value animal waste protein is prepared into functional polypeptide through a biotransformation technology, the functional polypeptide has the functions of adsorbing cations and chelating elements, then the functional polypeptide is used for modifying activated carbon, the functional polypeptide is organically mixed with other components according to a certain proportion to prepare the soil conditioner, and the functional polypeptide can be used as a fertilizer synergist in garden soil conservation and has a good application prospect. When the soil conditioner is used for treating the soil, nitrogen, phosphorus, potassium and trace elements in the soil can be enriched, the effectiveness of nutrient elements in the soil is improved, and the fixation of cadmium is reduced.

The present invention belongs to the field of enzyme engineering and food engineering technology, and specifically relates to the directed evolution and site-directed mutagenesis of a lactase enzyme molecule and its application in fresh milk. The amino acid sequence of the lactase mutant obtained by the present invention is shown in SEQ ID NO.4. Through the directed artificial evolution of the lactase molecule, a new lactase molecule is obtained that can efficiently convert lactose in fresh milk and synthesize oligosaccharides at a relatively low temperature, and is rapidly inactivated at a subsequent elevated temperature (such as pasteurization). This facilitates the highly nutritious in situ bioconversion of lactose in fresh milk into prebiotic oligosaccharides, further improving the nutritional value of fresh milk and expanding the fresh milk consumer group.

The invention discloses a Lactobacillus paracasei CCFM1455 strain, a polysaccharide prepared from the Lactobacillus paracasei CCFM1455 strain for promoting gastric mucosa repair and application of the Lactobacillus paracasei CCFM1455 strain in synergism with rhizoma polygonati, and belongs to the technical field of microorganisms and medicines. According to the lactobacillus paracasei CCFM1455 exopolysaccharide disclosed by the invention, by regulating an STAT3 / SOCS3 signal channel, the levels of gastric mucosal lesion markers PGE2 and TFF3 are remarkably reduced, the expression of inflammatory factors is regulated, and gastric barrier protection and inflammation relief are realized. In addition, when the strain is used for fermenting polygonatum sibiricum, double synergistic effects can be generated: (1) macromolecular substances such as polygonatum sibiricum saponin are biologically converted, and easily absorbed micromolecular active substances such as oleanolic acid and liukucoumarin are generated; (2) polygonatum polysaccharide is converted into high-activity compound polysaccharide, so that the mucous membrane repairing synergistic effect is enhanced; therefore, the lactobacillus paracasei CCFM1455 and the fermentation derivative thereof provide an innovative solution for targeted intervention of gastric mucosal lesion and modernization of traditional Chinese medicines.

The invention discloses a Chinese gall leaven preparation method based on constant-temperature fermentation, and relates to the technical field of Chinese gall leaven preparation. The preparation method comprises the following steps: respectively and independently cleaning gallnut, distiller's yeast of white spirit and tea water, and removing non-medicinal parts, mildewed medicinal materials and impurities; the method comprises the following steps: uniformly mixing gallnut powder, distiller's yeast of Baijiu and tea leaves according to a mass ratio of (10-15): (0.5-1): (2-4), adjusting the moisture content to 40-45%, and spreading the mixture in a material tray; according to the method, constant-temperature fermentation equipment is introduced, so that the temperature in the fermentation process is always kept within the range of 26 + / -5 DEG C for 60-72 hours, and drying, crushing and briquetting are performed. Compared with the traditional natural fermentation process, the constant-temperature fermentation has the advantages that the tannin biotransformation efficiency is remarkably improved, the tannin content is greatly reduced, the gallic acid content is greatly improved, the curative effect is greatly improved, the sour and astringent taste of the product is remarkably improved, the fermentation time is greatly shortened, the production efficiency is improved, and the energy cost is reduced.

The invention discloses an application of rhodococcus diploides in treatment of waste water containing isophorone, the rhodococcus diploides is classified and named as Rhodococcus tectodiphilus, the strain number is GXGD-YZ-2510, and the preservation number is GDMCC No: 67304. The invention further discloses a preparation method of the rhodococcus diploides. The strain shows good growth metabolism activity and isophorone degradation efficiency in a high-salt aerobic environment. Under the conditions that the system salinity is 1 wt%, the initial isophorone concentration is 200 mg / L, and the dissolved oxygen is 3-5 mg / L, the removal rate of the strain on the isophorone reaches 78.6%. The strain not only has excellent salt stress resistance, but also can realize efficient bioconversion of isophorone under high-salt aerobic conditions, and effectively solves the technical bottleneck that isophorone in high-salt wastewater in the industries of petrochemical engineering, chemical engineering, pharmacy and the like is difficult to biochemically treat; the method has the remarkable advantages of being thorough in degradation, low in cost, environmentally friendly, high in process adaptability and the like, and a novel strain resource and an industrialization technical scheme are provided for biological treatment of high-salinity wastewater of refractory organic pollutants.

The invention relates to the field of environment, resource utilization and agricultural biotechnology, in particular to a method for converting insoluble phosphorus by using enterobacter hormaechei microorganisms, a high-efficiency phosphate-solubilizing bacterial agent and application, and the high-efficiency phosphate-solubilizing bacterial agent comprises phosphate-solubilizing bacteria, bone black and a culture medium; the phosphate solubilizing bacteria are enterobacter hormaechei HS-6. By combining the enterobacter hormaechei HS-6 with the bone black, the phosphorus dissolving capacity of the enterobacter hormaechei HS-6 can be utilized, the phosphorus resource of the bone black can be fully utilized, the surface of the bone black can provide a growth and reproduction carrier for strains, and insoluble phosphorus can be converted into soluble phosphorus. The phosphate-solubilizing bacterial agent has good prospects in the application of biological bacterial fertilizer preparation, agricultural soil improvement, bone charcoal phosphorus resource recovery and crop growth promotion. The method for converting the insoluble phosphorus by using the enterobacter hormaechei microorganisms not only can effectively improve the utilization efficiency of phosphorus resources and reduce the dependence on limited phosphorite resources, but also has important significance for promoting agricultural sustainable development.

The invention provides a complex microbial inoculant and application of the complex microbial inoculant in degradation of kitchen waste in cooperation with hermetia illucens, belongs to the technical field of microorganism application, and provides bacillus subtilis LH-Pika23. The bacillus subtilis LH-Pika23 independently treats the kitchen waste and can remarkably remove odor in the kitchen waste. The complex microbial inoculant obtained by compounding the bacillus subtilis LH-Pika23 and the casei paracasei TDM-2 widens the variety of treatable substrates, and is higher in odor removal rate and more stable and lasting in odor removal effect. The complex microbial inoculant provided by the invention cooperates with the hermetia illucens to treat the kitchen waste, so that the bioconversion efficiency of the hermetia illucens is improved, and meanwhile, the complex microbial inoculant also has a remarkable deodorization effect.

The invention discloses a fresh plant directed biotransformation method based on endogenous enzyme activation and a product and application thereof. The core of the preparation method is as follows: fresh plants (tuberous roots, stems or fruits) which are harvested in a ripe state and have metabolism in a dormant state (the respiration rate is lower than 5 mg CO / kg.h) are taken as raw materials, the raw materials are treated in an environment with the specific pressure being 0.02-0.20 MPa, the temperature being 70-121 DEG C and the humidity being 50-95%, a specific impregnating compound can be selectively combined, and the raw materials can be prepared into the plant culture medium. Under the conditions that exogenous microorganisms are not introduced and plant tissue structures are not damaged, the catalytic function of an endogenous metabolic enzyme system is safely and efficiently restarted and maximally promoted, and components of a plant body are driven to realize directional biotransformation. According to the process, conversion of primary metabolites and / or secondary metabolites is remarkably promoted, the content conversion of one or more beneficial components in the primary metabolites and / or the secondary metabolites is improved, and meanwhile, the biological and pharmaceutical structures and inherent metabonomics characteristics of the plants are completely reserved. The biotransformation product can be used as a high-quality raw material to be applied to medicines, common foods, health foods, special diet foods, daily chemical products and disinfection products, and can be further prepared into traditional Chinese medicine decoction pieces, solid preparations, semi-solid preparations and liquid preparations by adding or not adding pharmaceutically acceptable auxiliary materials.

The invention discloses a melanin-producing aureobasidium for co-producing pullulan and converting American ginseng and application, and relates to the technical field of bioengineering fermentation. The melanin producing aureobasidium is aureobasidium melanogenum F8449, and the preservation number of the aureobasidium melanogenum F8449 is CGMCC (China General Microbiological Culture Collection Center) No. 23228. The strain can be used for simultaneously producing pullulan and biologically converting American ginseng extract in liquid, so that efficient utilization of fermentation substances is realized, the fermentation cost is reduced, and the fermentation income is increased.

The invention discloses a co-fermentation method based on sphingomonas and a rhodiola rosea extracting solution and application of a product, and belongs to the technical field of microorganisms. By utilizing a special rich enzyme system of sphingomonas and combining with an early-stage composite enzymolysis process and synergistic effect, the wall breaking efficiency of rhodiola rosea cell walls and the conversion degree of active components of rhodiola rosea are greatly improved, the bottlenecks of low efficiency and low bioavailability of a traditional rhodiola rosea extraction method are successfully overcome, and the rhodiola rosea extraction method is suitable for industrial production. The functional components such as salidroside and flavone can be fully released and converted. Secondly, different from non-symbiotic bacteria (such as lactic acid bacteria and saccharomycetes) which are commonly used for fermentation in the prior art, a potential symbiotic bacterium of sphingomonas XJL-TP07 from a special environment is innovatively selected and has better metabolic suitability with the rhodiola rosea component, the fermentation process is milder and safer (no irritant is produced), and the rhodiola rosea fermentation method is suitable for large-scale production. And a synergistic interaction effect can be generated through a unique biotransformation path.

The invention relates to a method for increasing the yield of glucose biologically converted from corn straws, which comprises the following steps of: carrying out composite pretreatment on the corn straws by using NaOH and urea, and then carrying out step-by-step enzymolysis, namely carrying out enzymolysis on the corn straws by using pleurotus eryngii lysate polysaccharide monooxygenase in the first step, and adding cellulase for further enzymolysis to produce the glucose in the second step, the pleuromutilus rubra lysate polysaccharide monooxygenase is a compound enzyme composed of PdLPMO9A and PdLPMO9B according to a ratio of (1: 2)-(1: 4). According to the method disclosed by the invention, the corn straws are subjected to specific pretreatment, the compound enzyme is formed by adopting the pleurotus eryngii lysate polysaccharide monooxygenase PdLPMO9A and PdLPMO9B according to a specific proportion, and the compound enzyme and the cellulase are used for performing enzymolysis on the corn straws step by step, so that the biological conversion efficiency of the corn straws is effectively improved under the same enzymolysis time, and the yield of glucose is improved and reaches 0.642 g / g of straws.

The invention discloses a culture base material for lepista sordida rich in strontium, zinc and germanium as well as a preparation method and application of the culture base material, and relates to the technical field of edible mushrooms. The culture base stock is prepared from the following raw materials: a nitrogen source, a carbon source, inorganic salt, spirulina, polyglutamic acid and compound bacteria, the compound bacteria comprise aspergillus niger, lactobacillus cavings-like and candida utilis; the culture base stock comprises trace elements; the trace elements comprise at least one of strontium, zinc and germanium. The culture base stock can effectively shorten the growth cycle of lepista sordida, improve the yield and the biotransformation rate, reduce the cost and promote enrichment of trace elements such as strontium, zinc and germanium in lepista sordida.

The invention relates to the technical field of sludge treatment, in particular to a sludge biological treatment system which comprises a fermentation box, the device further comprises an anaerobic fermentation area, an aerobic fermentation area, a precipitation tank plate, an aeration assembly, a transfer tank, a biological tank and a sludge outlet auger, the precipitation tank plate is in a V shape and installed in the middle of the fermentation box, the fermentation box is divided into the anaerobic fermentation area, the aerobic fermentation area and the precipitation area by the precipitation tank plate, the aeration assembly is installed at the bottom of the aerobic fermentation area, and the transfer tank is installed in the sludge outlet auger. The transfer tank is installed outside the fermentation box and communicated with a sludge outlet of the fermentation box, the lower end of the transfer tank is connected with the input end of the biological tank, the transfer tank is used for evaporating sludge moisture, a biological conversion bed is arranged in the biological tank, the biological tank is used for biological treatment of sludge, and the sludge outlet auger is installed outside the biological tank. The input end of the sludge outlet auger is connected with the output end of the biological tank; the high-integration fermentation box is adopted, the fermentation speed is high, fermentation is more sufficient, sludge can be preliminarily separated, and the subsequent biological treatment efficiency is improved.

The invention belongs to the technical field of fungus cultivation, and particularly relates to a cultivation method of high-yield and high-quality phellinus igniarius. The cultivation method comprises the following steps: (1) inoculating: preparing a culture medium, putting the culture medium into a plastic bag, sterilizing, cooling, and inoculating by using a phellinus igniarius strain; (2) spawn running management: culturing the inoculated plastic bag in a dark place; after 30-35 days of spawn running management, longitudinally extruding and shortening the fungus bags, continuing to culture, and entering fruiting management; (3) fruiting management: transferring the maturely cultured phellinus igniarius fungus bags into a fruiting greenhouse, marking rectangular fruiting openings in the middle parts of the fungus bags, and carrying out phellinus igniarius fruiting management; and (4) harvesting: obtaining the phellinus igniarius sporocarp 100-120 days after cutting. According to the cultivation method for the high-yield and high-quality phellinus igniarius, the biotransformation efficiency and the content of effective components of the phellinus igniarius are remarkably improved by optimizing the components and the proportion of the culture medium, and the technical problems that in traditional cultivation, the yield is low, and the content of the effective components is low are solved.

This invention relates to the fields of environmental protection, resource utilization, and agricultural biotechnology, specifically to a method for converting insoluble phosphorus using *Enterobacter horneri* microorganisms, as well as a highly efficient phosphorus-solubilizing agent and its applications. The highly efficient phosphorus-solubilizing agent comprises phosphorus-solubilizing bacteria, bone char, and a culture medium; the phosphorus-solubilizing bacteria is *Enterobacter horneri* HS-6. By combining *Enterobacter horneri* HS-6 with bone char, both the phosphorus-solubilizing ability of *Enterobacter horneri* HS-6 and the phosphorus resources of bone char can be fully utilized. The surface of bone char provides a carrier for the growth and reproduction of the microorganisms, and its insoluble phosphorus can be converted into soluble phosphorus. This phosphorus-solubilizing agent shows great promise in the preparation of bio-fertilizers, agricultural soil improvement, bone char phosphorus resource recovery, and the promotion of crop growth. This method for converting insoluble phosphorus using *Enterobacter horneri* microorganisms not only effectively improves the utilization efficiency of phosphorus resources and reduces dependence on limited phosphate rock resources, but also has significant implications for promoting sustainable agricultural development.

A probiotic and prebiotic-embedded synbiotic system for targeted delivery and colonization of functions, comprising a probiotic core; an inner layer of protein-polysaccharide / oligosaccharide complex wrapped around the probiotic surface, which acts as both an adhesion promoter, promoting the attachment of probiotics to the intestinal epithelial cells, and as a prebiotic, promoting the growth of probiotics upon release; and an outer layer of a natural emulsifying system on the synbiotic complex, which minimizes water infiltration, thereby effectively embedding and protecting the probiotics. The present synbiotic system is suitable for production in powder form using freeze-drying technology, and is suitable as a dietary supplement or for addition to any food in dry powder form. The system has the ability to bioconvert soy isoflavones and release bioactive phenolic acids from grains to promote body health.

The utility model relates to the technical field of machine waste bioconversion, in particular to hermetia illucens kitchen waste treatment equipment which comprises an incubation box body, a feeding hopper is arranged in the middle of the top of the incubation box body, a rectangular guide groove is formed in the inner wall of the rear side of the incubation box body, and driving blocks capable of moving transversely are symmetrically arranged in the rectangular guide groove. The outer side of the driving block is rotationally connected with a movable scattering plate, a built-in overhead plate is arranged at the bottom of the incubation box body, the four corners of the bottom of the built-in overhead plate are connected with the inner bottom wall of the incubation box body through supporting columns, and dynamic ventilation mechanisms are arranged on the two sides of the incubation box body. According to the hermetia illucens kitchen waste treatment equipment, uniform distribution and good ventilation of kitchen waste in the hatching area can be achieved, the feeding efficiency and growth speed of hermetia illucens larvae are remarkably improved, meanwhile, equipment maintenance is simplified, and the stability and efficiency of the treatment process are enhanced.

The present invention relates to a bioconversion for elongating gluco-oligosaccharides with a glucosyl moiety. The present invention also relates to the elongated gluco-oligosaccharides obtained by the process. The elongated gluco-oligosaccharides obtained by the process can be used in edible products, for instance as a dietary fiber or a (low calorie) bulking agent, a prebiotic, in sugar replacement or in calorie reduction. The elongated gluco-oligosaccharides obtained by the process present a decreased digestibility compared to known gluco-oligosaccharides.

The present invention provides a construction method of Halomonas J9U and its derivatives, a PHA preparation method and application, and belongs to the field of genetic engineering technology. The present invention connects the upstream and downstream homology arm sequences of the upp gene with the suicide plasmid pK18mobsacB to obtain a targeting vector pK18Δupp, which is transferred into the aspiring Halomonas J9, and two homologous recombinations occur to obtain J9U. The homologous recombination efficiency of this construction method is extremely high, up to 100%. The maximum yield of PHA produced by the J9UΔxylD‑P8xylA strain using xylose as the sole carbon source is 2.81g / L, and the strain is an advantageous chassis strain in the co-utilization of xylose and glucose. It has superior growth and PHA yield advantages in lignocellulose bioconversion, greatly reducing production costs.

The application discloses an improved plant liquid culture medium, which comprises inorganic nutrients required by plant basic growth and does not comprise compounds containing an EDTA ligand skeleton and iodide. The application also discloses application of the improved plant liquid culture medium in mercury compound metabolism research. The application is based on the technical problem of finding that a culture medium substrate mediates abiotic transformation and causes error defects, and by eliminating the compounds containing the EDTA ligand skeleton and the iodide which induce abiotic transformation of mercury compounds, the application is applied to block the abiotic transformation path of methyl mercury in the mercury compound metabolism research, and eliminate the 'false positive' error in the metabolism research.

The invention relates to the field of solid waste resource utilization and soil remediation, and discloses industrial equipment for graded utilization of solid wastes, which comprises a raw material pretreatment unit, a multi-stage bioconversion unit, an intelligent regulation and control unit, a flue gas purification unit, a sewage treatment unit and a finished product treatment unit, the multi-stage bioconversion comprises an anaerobic fermentation tank I stage, an aerobic composting bin II stage, an after-ripening aging tank III stage and 45-degree inclined flow guide pipes which are connected in series according to the volume ratio of 1: 2: 1; the intelligent regulation and control comprises multi-parameter online monitoring, LoRa transmission and PID (Proportion Integration Differentiation) regulation; through the design, continuous and efficient conversion of solid waste, zero emission in the whole process and output of standard soil and remediation materials are realized by combining microbial metabolic flux regulation and control, so that the problems of landfill saturation, secondary pollution caused by incineration, low single-stage treatment efficiency, lack of intelligent regulation and separation of solid waste treatment and soil remediation in the existing solid waste treatment are solved.

The present application relates to the technical field of traditional Chinese medicine, in particular to a preparation method of a tea-substitute formula for treating insomnia in young and middle-aged people, which comprises the following steps: crushing fried jujube seeds, cleaning and draining dried yam, dried red mulberry and mixing them, mixing and stirring the plant lactobacillus powder and the medicinal materials, constant temperature fermentation and low temperature drying after sealing, coarse grinding after drying, mixing with sweet hibiscus and sweet marigold to obtain the tea-substitute formula. The tea-substitute formula for treating insomnia in young and middle-aged people and the preparation method thereof mainly use fried jujube seeds to nourish the heart and calm the nerves, yam to calm the heart and calm the nerves, and fuling to invigorate the spleen and calm the heart as auxiliary, and combine dried red mulberry to nourish yin and tonify blood, sweet hibiscus to relieve depression and calm the nerves, and sweet marigold to harmonize the taste, so as to regulate the balance of neurotransmitters from multiple targets, regulate sleep as a whole, and use the enzymes produced by microorganisms to destroy the cell wall of traditional Chinese medicine, so as to effectively improve the dissolution rate and biological conversion rate of sleep-aiding effective components such as jujube saponin and lily alkaloids.

The invention discloses a method for regulating and controlling efficient conversion of methanol based on (p) ppGpp signal molecules. Specifically, by regulating synthesis and hydrolysis balance of (p) ppGpp signal molecules in microbial cells and combining promoter engineering and nutrition condition optimization, methanol metabolic pathway key gene expression and amino acid metabolic flux are enhanced, and the methanol utilization efficiency and target product synthesis capacity of microorganisms are remarkably improved. The regulation and control mechanism of the (p) ppGpp on methanol conversion under the stress of a low nitrogen source is defined for the first time, efficient conversion of methanol to organic acids such as acetic acid and butyric acid is realized, a brand new strategy is provided for preparation of high-added-value chemicals through methanol bioconversion, and the method has the advantages of being economical, environmentally friendly and high in conversion efficiency and has a wide application prospect.

A process includes providing a gaseous substrate comprising CO2 to a bioreactor; providing acetogenic bacteria and medium to the bioreactor to provide a fermentation broth; providing sodium ions to the bioreactor through one or more sodium ion sources; fermenting the gaseous substrate with the acetogenic bacteria in the fermentation broth to produce one or more organic acids; and controlling a butyric acid to an acetic acid ratio by controlling the pH of the fermentation broth. In one aspect, butyric acid to acetic acid ratio increases when the pH of the fermentation broth decreases, and the ratio of butyric acid to acetic acid concentration decreases when the pH of the fermentation broth increases. The acetogenic bacteria includes a sodium translocating ATPase that is active during fermentation in the bioreactor. The sodium ions are provided so that Na+ is maintained between 1000 to 11000 ppm (g / g) in culture broth.

This invention discloses a highly active Lactobacillus acidophilus strain that produces high levels of organic selenium, belonging to the field of microbial technology. The Lactobacillus acidophilus CCFM1304 obtained by screening in this invention exhibits high activity and can efficiently enrich inorganic selenium, converting it into organic selenium for better absorption and utilization by the body. Specifically: (1) Lactobacillus acidophilus CCFM1304 can efficiently enrich inorganic selenium, converting it into organic selenium with an organic selenium content of 95.3%, and the content of selenocysteine ​​in the organic selenium is as high as 67.8%; (2) Lactobacillus acidophilus CCFM1304 has high activity, and the viable count of the freeze-dried bacterial powder can reach 6.5 × 10⁻⁶. 11 CFU / g. The highly active Lactobacillus CCFM1304 of this invention can efficiently enrich inorganic selenium, and has great application prospects in the field of inorganic selenium biotransformation.

The invention relates to the technical field of functional foods, in particular to a fermented cereal formula food for controlling sugar and a preparation method thereof, three strains synergistically ferment cereals, and are compounded with a high-activity white hyacinth bean alpha-amylase inhibitor, high-purity mulberry leaf superfine powder and chromium-enriched yeast to jointly construct a multi-way blood sugar regulation and control system; according to the formula, three proteins of soybeans, whey and peas are combined with dietary fibers and prebiotics, the nutritional structure is optimized, satiety is enhanced, traditional herbal essences such as bitter gourds and Chinese yams are supplemented, the dual goals of scientific sugar control and nutrition balance are achieved through precise proportioning, deep biotransformation of cereal nutritional ingredients is achieved through a three-strain staged synergistic fermentation technology, and the nutritional value of the whole grain is improved. According to the present invention, the deep biological conversion of cereal nutritional components is achieved through the protein hydrolysis and glycoside hydrolysis ability of the lactobacillus plantarum, the glutamic acid decarboxylation ability of the bifidobacterium breve, and the vitamin synthesis and chromium enrichment ability of the saccharomyces cerevisiae;

The present application belongs to the technical field of microorganisms, and particularly relates to a Limosilactobacillus reuteri HP-B1251 and application thereof. The Limosilactobacillus reuteri HP-B1251 provided by the present application has been preserved in the China Center for Type Culture Collection on August 28, 2024, and the preservation number is CCTCC NO: M 20241872. The strain has good gastric acid tolerance, high temperature tolerance, bile salt tolerance, good acid production capacity, physiological and biochemical capacity suitable for the gastrointestinal tract of the body, and the ability to convert common ginsenosides into rare ginsenosides. At the same time, the biological conversion method has the advantages of strong reaction selectivity, mild conditions, few by-products, simple subsequent treatment process, small environmental pollution, and the like, and is an important way for industrialized production of rare ginsenosides.

Method to produce an upgraded oil using a lignin-rich feedstock, said method comprises the following steps: - providing a lignin-rich feedstock, wherein said lignin-rich feedstock comprises more than 60 wt% of lignin-based compounds obtained from delignification of biomass, where said lignin-based compounds are selected from the group consisting of: lignin-derived monomers, lignin-derived dimers, lignin-derived oligomers and combinations thereof; - providing an acidic composition having a pH of less than 1, said acidic composition comprising:  an acid selected from the group consisting of: sulfuric acid; an alkylsulfonic acid; and an arylsulfonic acid; and  an alcohol selected from the group consisting of C1-C8 linear alcohol and C3-C8 branched alcohol and mixtures thereof; - combining said lignin-rich feedstock with said acidic composition into a reaction mixture; - heating up said mixture to a temperature ranging from 25oC to 120oC; and - allowing sufficient time of reaction to convert at least a portion of said lignin-derived material into said at least one esterified lignin derivative; - separating said at least one esterified lignin derivative from said acidic composition; - performing a hydrodeoxygenation reaction on said at least one esterified lignin derivative, wherein the hydrodeoxygenation reaction is carried out in the presence of a hydrogen-rich source at a temperature ranging from 250oC to 400oC under a H2 pressure ranging from 15 to 50 bar, more preferably 35 bar, in the presence of a catalyst adapted for HDO reactions, for a period of time sufficient to result in an upgraded oil having a TAN of about 2.5 mg KOH / g and viscosity of 3.4 cP.