141 results about "Bioconversion" patented technology

The invention relates to the technical field of biological catalysis, and discloses an M88V mutant enzyme for preparing rebaudioside I. The M88V mutant enzyme is obtained by the following mutations generated by UGT76G1: M88V: methionine of the 88th amino acid sequence of UGT76G1 is mutated into valine. Aiming at the technical bottlenecks of low RI yield and poor selectivity in the existing RA biotransformation process, an efficient solution is provided for the UGT76G1 mutant developed by the invention. The mutant can specifically catalyze conversion from RA to RI, and the conversion rate of a target product is remarkably increased to 65% or above from general less than 7%. The excellent catalytic performance of the catalyst is verified in milliliter-level to upgraded reaction systems, the key problems of conversion efficiency and process amplification are solved, and a foundation is laid for large-scale green manufacturing of a high-added-value product RI.

The invention discloses an application of rhodococcus diploides in treatment of waste water containing isophorone, the rhodococcus diploides is classified and named as Rhodococcus tectodiphilus, the strain number is GXGD-YZ-2510, and the preservation number is GDMCC No: 67304. The invention further discloses a preparation method of the rhodococcus diploides. The strain shows good growth metabolism activity and isophorone degradation efficiency in a high-salt aerobic environment. Under the conditions that the system salinity is 1 wt%, the initial isophorone concentration is 200 mg / L, and the dissolved oxygen is 3-5 mg / L, the removal rate of the strain on the isophorone reaches 78.6%. The strain not only has excellent salt stress resistance, but also can realize efficient bioconversion of isophorone under high-salt aerobic conditions, and effectively solves the technical bottleneck that isophorone in high-salt wastewater in the industries of petrochemical engineering, chemical engineering, pharmacy and the like is difficult to biochemically treat; the method has the remarkable advantages of being thorough in degradation, low in cost, environmentally friendly, high in process adaptability and the like, and a novel strain resource and an industrialization technical scheme are provided for biological treatment of high-salinity wastewater of refractory organic pollutants.

The invention discloses a fresh plant directed biotransformation method based on endogenous enzyme activation and a product and application thereof. The core of the preparation method is as follows: fresh plants (tuberous roots, stems or fruits) which are harvested in a ripe state and have metabolism in a dormant state (the respiration rate is lower than 5 mg CO / kg.h) are taken as raw materials, the raw materials are treated in an environment with the specific pressure being 0.02-0.20 MPa, the temperature being 70-121 DEG C and the humidity being 50-95%, a specific impregnating compound can be selectively combined, and the raw materials can be prepared into the plant culture medium. Under the conditions that exogenous microorganisms are not introduced and plant tissue structures are not damaged, the catalytic function of an endogenous metabolic enzyme system is safely and efficiently restarted and maximally promoted, and components of a plant body are driven to realize directional biotransformation. According to the process, conversion of primary metabolites and / or secondary metabolites is remarkably promoted, the content conversion of one or more beneficial components in the primary metabolites and / or the secondary metabolites is improved, and meanwhile, the biological and pharmaceutical structures and inherent metabonomics characteristics of the plants are completely reserved. The biotransformation product can be used as a high-quality raw material to be applied to medicines, common foods, health foods, special diet foods, daily chemical products and disinfection products, and can be further prepared into traditional Chinese medicine decoction pieces, solid preparations, semi-solid preparations and liquid preparations by adding or not adding pharmaceutically acceptable auxiliary materials.

The invention discloses a melanin-producing aureobasidium for co-producing pullulan and converting American ginseng and application, and relates to the technical field of bioengineering fermentation. The melanin producing aureobasidium is aureobasidium melanogenum F8449, and the preservation number of the aureobasidium melanogenum F8449 is CGMCC (China General Microbiological Culture Collection Center) No. 23228. The strain can be used for simultaneously producing pullulan and biologically converting American ginseng extract in liquid, so that efficient utilization of fermentation substances is realized, the fermentation cost is reduced, and the fermentation income is increased.

The invention discloses a co-fermentation method based on sphingomonas and a rhodiola rosea extracting solution and application of a product, and belongs to the technical field of microorganisms. By utilizing a special rich enzyme system of sphingomonas and combining with an early-stage composite enzymolysis process and synergistic effect, the wall breaking efficiency of rhodiola rosea cell walls and the conversion degree of active components of rhodiola rosea are greatly improved, the bottlenecks of low efficiency and low bioavailability of a traditional rhodiola rosea extraction method are successfully overcome, and the rhodiola rosea extraction method is suitable for industrial production. The functional components such as salidroside and flavone can be fully released and converted. Secondly, different from non-symbiotic bacteria (such as lactic acid bacteria and saccharomycetes) which are commonly used for fermentation in the prior art, a potential symbiotic bacterium of sphingomonas XJL-TP07 from a special environment is innovatively selected and has better metabolic suitability with the rhodiola rosea component, the fermentation process is milder and safer (no irritant is produced), and the rhodiola rosea fermentation method is suitable for large-scale production. And a synergistic interaction effect can be generated through a unique biotransformation path.

A probiotic and prebiotic-embedded synbiotic system for targeted delivery and colonization of functions, comprising a probiotic core; an inner layer of protein-polysaccharide / oligosaccharide complex wrapped around the probiotic surface, which acts as both an adhesion promoter, promoting the attachment of probiotics to the intestinal epithelial cells, and as a prebiotic, promoting the growth of probiotics upon release; and an outer layer of a natural emulsifying system on the synbiotic complex, which minimizes water infiltration, thereby effectively embedding and protecting the probiotics. The present synbiotic system is suitable for production in powder form using freeze-drying technology, and is suitable as a dietary supplement or for addition to any food in dry powder form. The system has the ability to bioconvert soy isoflavones and release bioactive phenolic acids from grains to promote body health.

The present invention relates to a bioconversion for elongating gluco-oligosaccharides with a glucosyl moiety. The present invention also relates to the elongated gluco-oligosaccharides obtained by the process. The elongated gluco-oligosaccharides obtained by the process can be used in edible products, for instance as a dietary fiber or a (low calorie) bulking agent, a prebiotic, in sugar replacement or in calorie reduction. The elongated gluco-oligosaccharides obtained by the process present a decreased digestibility compared to known gluco-oligosaccharides.

The application discloses an improved plant liquid culture medium, which comprises inorganic nutrients required by plant basic growth and does not comprise compounds containing an EDTA ligand skeleton and iodide. The application also discloses application of the improved plant liquid culture medium in mercury compound metabolism research. The application is based on the technical problem of finding that a culture medium substrate mediates abiotic transformation and causes error defects, and by eliminating the compounds containing the EDTA ligand skeleton and the iodide which induce abiotic transformation of mercury compounds, the application is applied to block the abiotic transformation path of methyl mercury in the mercury compound metabolism research, and eliminate the 'false positive' error in the metabolism research.

The invention relates to the field of solid waste resource utilization and soil remediation, and discloses industrial equipment for graded utilization of solid wastes, which comprises a raw material pretreatment unit, a multi-stage bioconversion unit, an intelligent regulation and control unit, a flue gas purification unit, a sewage treatment unit and a finished product treatment unit, the multi-stage bioconversion comprises an anaerobic fermentation tank I stage, an aerobic composting bin II stage, an after-ripening aging tank III stage and 45-degree inclined flow guide pipes which are connected in series according to the volume ratio of 1: 2: 1; the intelligent regulation and control comprises multi-parameter online monitoring, LoRa transmission and PID (Proportion Integration Differentiation) regulation; through the design, continuous and efficient conversion of solid waste, zero emission in the whole process and output of standard soil and remediation materials are realized by combining microbial metabolic flux regulation and control, so that the problems of landfill saturation, secondary pollution caused by incineration, low single-stage treatment efficiency, lack of intelligent regulation and separation of solid waste treatment and soil remediation in the existing solid waste treatment are solved.

The invention discloses a method for regulating and controlling efficient conversion of methanol based on (p) ppGpp signal molecules. Specifically, by regulating synthesis and hydrolysis balance of (p) ppGpp signal molecules in microbial cells and combining promoter engineering and nutrition condition optimization, methanol metabolic pathway key gene expression and amino acid metabolic flux are enhanced, and the methanol utilization efficiency and target product synthesis capacity of microorganisms are remarkably improved. The regulation and control mechanism of the (p) ppGpp on methanol conversion under the stress of a low nitrogen source is defined for the first time, efficient conversion of methanol to organic acids such as acetic acid and butyric acid is realized, a brand new strategy is provided for preparation of high-added-value chemicals through methanol bioconversion, and the method has the advantages of being economical, environmentally friendly and high in conversion efficiency and has a wide application prospect.

This invention discloses a highly active Lactobacillus acidophilus strain that produces high levels of organic selenium, belonging to the field of microbial technology. The Lactobacillus acidophilus CCFM1304 obtained by screening in this invention exhibits high activity and can efficiently enrich inorganic selenium, converting it into organic selenium for better absorption and utilization by the body. Specifically: (1) Lactobacillus acidophilus CCFM1304 can efficiently enrich inorganic selenium, converting it into organic selenium with an organic selenium content of 95.3%, and the content of selenocysteine ​​in the organic selenium is as high as 67.8%; (2) Lactobacillus acidophilus CCFM1304 has high activity, and the viable count of the freeze-dried bacterial powder can reach 6.5 × 10⁻⁶. 11 CFU / g. The highly active Lactobacillus CCFM1304 of this invention can efficiently enrich inorganic selenium, and has great application prospects in the field of inorganic selenium biotransformation.

Method to produce an upgraded oil using a lignin-rich feedstock, said method comprises the following steps: - providing a lignin-rich feedstock, wherein said lignin-rich feedstock comprises more than 60 wt% of lignin-based compounds obtained from delignification of biomass, where said lignin-based compounds are selected from the group consisting of: lignin-derived monomers, lignin-derived dimers, lignin-derived oligomers and combinations thereof; - providing an acidic composition having a pH of less than 1, said acidic composition comprising:  an acid selected from the group consisting of: sulfuric acid; an alkylsulfonic acid; and an arylsulfonic acid; and  an alcohol selected from the group consisting of C1-C8 linear alcohol and C3-C8 branched alcohol and mixtures thereof; - combining said lignin-rich feedstock with said acidic composition into a reaction mixture; - heating up said mixture to a temperature ranging from 25oC to 120oC; and - allowing sufficient time of reaction to convert at least a portion of said lignin-derived material into said at least one esterified lignin derivative; - separating said at least one esterified lignin derivative from said acidic composition; - performing a hydrodeoxygenation reaction on said at least one esterified lignin derivative, wherein the hydrodeoxygenation reaction is carried out in the presence of a hydrogen-rich source at a temperature ranging from 250oC to 400oC under a H2 pressure ranging from 15 to 50 bar, more preferably 35 bar, in the presence of a catalyst adapted for HDO reactions, for a period of time sufficient to result in an upgraded oil having a TAN of about 2.5 mg KOH / g and viscosity of 3.4 cP.

The invention belongs to the technical field of biological bacterial fertilizer preparation, and relates to a composite wormcast biological bacterial fertilizer and a preparation and use method thereof. Mixing and pre-fermenting the modified cow dung and the modified corn straw; performing gradient inoculation on adult and young earthworms for biotransformation to obtain composite wormcast; carrying out enzymolysis on an organic nitrogen source, and compounding with humic acid to prepare a stable nitrogen source solution; and finally, mixing the composite wormcast, a nitrogen source liquid and a specific bacillus amyloliquefaciens inoculant in proportion, and carrying out stepped temperature-controlled fermentation to obtain a finished product. According to the method, through raw material biological modification, earthworm gradient conversion, nutrient enzymolysis compounding and functional bacterium directional fermentation multi-stage cooperation, the survival rate and activity of functional bacteria in the product and the soil colonization capacity are remarkably improved, nutrient slow release and supply and demand matching are achieved, and the functions of nutrient supply, growth promotion and disease resistance and soil improvement are integrated.

This invention relates to the field of insect resource utilization and agricultural waste treatment technology, specifically to a high-linoleic acid black soldier fly oil, black soldier fly larvae, and their applications. The method for raising black soldier fly larvae includes the following steps: black soldier fly eggs are hatched and placed in a hatching substrate for rearing; after obtaining larvae, they are inoculated into a culture feed and then harvested to obtain black soldier fly larvae; high-linoleic acid black soldier fly oil is further extracted. This invention provides a novel insect-based source for obtaining linoleic acid by precisely matching the feed substrate and harvesting time, without relying on traditional plant oil crops. During the high-linoleic acid oil harvesting window, the crude protein content of black soldier fly larvae remains at a relatively high level of 58%~65%. The wheat bran and cassava residue used in this invention are inexpensive and widely available agricultural processing by-products, resulting in low production costs and achieving resource utilization of waste through bioconversion.

The invention discloses a black soldier fly larva biotransformation treatment method of blue-green algae biomass and a pre-fermentation mixed substrate. The method comprises the following steps: mixing blue-green algae biomass with cow dung to obtain a mixed substrate; adjusting the mass ratio of carbohydrate to protein in the mixed substrate to 3: 1-5: 1 according to the dry basis of the mixed substrate, and adjusting the water content of the mixed substrate to 70%-90%; placing the mixed substrate in a closed container, and pre-fermenting for 5-15 days under an anoxic or anaerobic condition to obtain a pre-fermented mixed substrate; inoculating hermetia illucens larvae into the pre-fermented mixed substrate for feeding transformation; and after the feeding transformation is finished, separating to obtain polypide bodies and residual substrates. The stability of feeding transformation can be improved and the dependence on exogenous additives can be reduced by regulating and controlling the nutritional structure on the mixed substrate level and implementing closed pre-fermentation. The pre-fermented mixed substrate is obtained by pre-fermenting the mixed substrate.

The invention relates to the technical field of lignocellulose biomass pretreatment, in particular to a method for quickly and efficiently hydrolyzing lignocellulose biomass by utilizing biological micro-aerobic treatment. According to the method, a specific dosage of calcium peroxide (CaO2) is added into a mixed system containing lignocellulose biomass and inoculated sludge, and a micro-aerobic condition created by decomposition of the calcium peroxide and a synergistic effect of microbial communities in the inoculated sludge are utilized, so that facultative hydrolytic bacteria are directionally enriched, and the activity of hydrolase is up-regulated; the complex structure of the lignocellulose is efficiently destroyed. Compared with an existing micro-aerobic pretreatment technology depending on a high-dose chemical oxidizing agent, the method has the advantages that microbial regulation is taken as a core, the dosage of a chemical agent and residual oxidizing substances are greatly reduced while efficient hydrolysis of lignocellulose is realized, inhibition of the chemical agent on the subsequent biotransformation process is effectively avoided, and the method is suitable for industrial production. And an efficient, economic and environment-friendly pretreatment solution is provided for full-chain resource utilization of lignocellulose biomass.

This invention relates to the field of edible fungi technology, providing a method for breeding enoki mushrooms. The method includes: collecting enoki mushroom strain samples from different regions; conducting morphological observations on the collected strains; culturing the strains on standard culture media; testing the strains' resistance to diseases and pests; selecting strains with fast growth rates, high bioconversion rates, and strong resistance to diseases and pests for hybridization; screening the hybrid offspring to select individuals with round caps, straight stems, and pure white color; purifying the selected individuals through self-pollination; and expanding the cultivation of the finally selected superior strains for wider application and promotion. Through precise control of environmental parameters and strict selection criteria, enoki mushroom varieties with superior traits can be bred more efficiently and accurately, resulting in significant improvements in growth rate, morphological quality, and stress resistance.

This invention concerns a household organic waste processing technology that integrates aerobic fermentation and solid residue bioconversion processes in one system. This invention is a liquid organic fertilizer reactor based on used drums equipped with an aeration system, a liquid and solid phase separator, and a liquid organic fertilizer outlet valve. Organic waste is fermented aerobically with the help of a bioactivator to produce liquid organic fertilizer, while the resulting solid residue is used as a cultivation medium for Hermetia illucens maggots. This integration enables zero-waste organic waste processing, producing economically valuable products in the form of liquid organic fertilizer, maggot biomass, and solid residue as organic fertilizer. This invention is simple, economical, easy to apply on a household scale, and environmentally friendly.

The invention discloses a food waste cooperative processing system based on multi-objective optimization. The food waste cooperative processing system comprises a data acquisition and processing module, a multi-objective optimization decision module and a process cooperative control module, the data acquisition and processing module is used for collecting real-time operation data of a plurality of treatment processes, and the real-time operation data at least comprises gas production rate of anaerobic digestion, temperature change data of a composting process, feed output rate of bioconversion of hermetia illucens, regional food waste characteristic data, policy requirements and market demand data; based on the data acquired by the data acquisition and processing module, establishing a multi-objective optimization model taking the minimization of the total greenhouse gas emission of the system and the maximization of the total economic income of the system as objectives, and outputting a garbage distribution scheme; a multi-objective optimization decision module dynamically adjusts the garbage distribution amount of each treatment process according to the garbage distribution scheme output by the multi-objective optimization model; and through intelligent resource allocation, the existing treatment processes are optimally combined, and the garbage co-treatment efficiency is improved.

The application discloses a 7beta-hydroxysteroid dehydrogenase mutant and application thereof, relates to the field of bioengineering technology, and is designed and reformed according to the amino acid sequence of wild-type 7beta-hydroxysteroid dehydrogenase derived from Collinsella aerofaciens, that is, 17 single-mutation sites or 7 combined-mutation sites of 7beta-hydroxysteroid dehydrogenase are provided, the obtained 7beta-hydroxysteroid dehydrogenase mutant has the thermal stability increased by 2-21.5 DEG C and the activity increased by nearly 1.2-2.24 times compared with the wild-type 7beta-hydroxysteroid dehydrogenase. According to the mutation sites with obvious increase in thermal stability and activity, three preferred mutant proteins are further provided, the three preferred mutant proteins have higher yield, activity and thermal stability compared with the wild-type 7beta-hydroxysteroid dehydrogenase, have wider application conditions, are more suitable for the efficient production of ursodeoxycholic acid by the bioconversion method, and are favorable for large-scale production and industrial application.

The application belongs to the technical field of bioactive substance preparation and agricultural application, and discloses a beta-amino butyric acid, a whole cell conversion preparation method thereof and application thereof. The method uses Paenibacillus halophilus as a catalyst to perform biological conversion in a reaction system containing a substrate, so as to obtain the beta-amino butyric acid. The method has mild reaction conditions, the bacterial cells can be repeatedly used, the conversion rate is high, and the green, efficient and low-cost production of the beta-amino butyric acid is realized. The application also provides application of the beta-amino butyric acid prepared by the above method in improving salt stress resistance of crops and in plant disease control as a pesticide synergist. Experiments show that the beta-amino butyric acid can significantly alleviate the inhibition of salt stress on the growth of wheat, and improve the chlorophyll content and biomass accumulation; the beta-amino butyric acid has a synergistic effect on grape diseases when used in combination with anilazodifen and fluoroacetamide, and meanwhile, the fruit quality and storage resistance are improved.

The application discloses high-temperature-resistant engineered methanotrophs with high glutamic acid yield, a construction method and application thereof, and belongs to the field of industrial biotechnology. Methylotuvimicrobium buryatense 5GB1 as the host strain, transcriptional inhibition of the SOS response system by LexA protein is removed by knocking out a lexA gene in the genome of the host strain, expression of DNA damage repair related genes in the cell is activated, and high-temperature-resistant engineered methanotrophs MBΔ lexA that can stably grow in a high-temperature environment of 40-45 DEG C are obtained. The strain can still maintain normal growth and metabolism with methane as the sole carbon source under the condition of 40-45 DEG C, and the glutamic acid synthesis capacity of the strain is significantly better than that of the original strain at normal temperature. The high-temperature-resistant engineered methanotrophs MBΔ lexA provide important strain resources for the production of amino acid products by methanotroph cell factories in a high-temperature industrial scene, and are expected to improve the economy and sustainability of methanotrophic bioconversion.

Disclosed herein are methods for oxidative catalytic depolymerization of polystyrene (PS) can produce benzoic acid, but the annual consumption of benzoic acid is about 40 times lower than PS. For this catalytic oxidation method to be a viable means to manage PS waste, benzoic acid should be converted to higher-volume chemicals. We demonstrate a hybrid chemical and biological process that uses PS as feedstock for production of adipic acid, a high-volume co-monomer for nylon 6,6 via benzoic acid. Mn / Br co-catalyzed autoxidation of PS to benzoic acid proceeds with a yield of up to 94% in a solvent mixture of benzoic acid and water. The PS-derived benzoic acid undergoes bioconversion at near-quantitative yield to muconic acid, which is readily converted to adipic acid through catalytic hydrogenation.

The present invention relates to novel steviol glycosides R6-1, R6-2A, R6-2B, R6-4A, R6-4B and R7-2 and the production of these novel steviol glycosides, such as through enzymatic bioconversion. The use of these novel steviol glycosides as sweeteners and in orally consumable products are also provided.

The invention provides a preparation method of a microbial fermentation five-flavor mannan medicated bath lotion, and belongs to the technical field of fermentation processes. The preparation method comprises the following steps: crushing Wuwei ganlu medicated bath raw materials, and carrying out wall breaking treatment through steam explosion to obtain a steam explosion substrate; adjusting the water content of the steam explosion substrate, inoculating enzyme-producing fungi to carry out aerobic solid-state fermentation, and carrying out enzymolysis on the substrate by utilizing an extracellular enzyme system secreted by the fungi to obtain a solid-state fermentation product; adding water into the solid-state fermentation product, inoculating transformed bacteria and saccharomycetes, carrying out anaerobic liquid-state fermentation, forming a water-alcohol-acid mixed system through microbial metabolism, and transforming and extracting active components to obtain fermented mash; and carrying out solid-liquid separation on the fermented mash, and carrying out membrane separation, filtration, sterilization and impurity removal on the obtained liquid to obtain the microbial fermentation five-flavor mannan medicated bath liquid. Through steam explosion physical modification and solid-liquid composite fermentation biotransformation, effective dissociation of medicinal material cell walls and improvement of substrate utilization rate are realized.

The invention discloses a method for producing 11 alpha-hydroxycanrenone by efficiently converting canrenone, which comprises the following steps: inoculating recombinant pichia pastoris into a shake flask filled with a seed culture medium, and culturing until the OD600 value of a bacterial solution reaches 8-10 to obtain a seed solution; inoculating the seed solution into a fermentation tank filled with a fermentation culture medium according to the inoculum size of 3-8%, and carrying out fermentation culture; the fermentation culture medium contains inositol; carrying out fermentation culture until dissolved oxygen rises again, and starting to add methanol in a fed-batch manner to carry out induced expression; and then adding canrenone into the fermentation tank, and ending fermentation until the canrenone conversion rate reaches 90% or above. Recombinant pichia pastoris microorganisms are adopted to convert canrenone to prepare 11 alpha-hydroxy canrenone, meanwhile, components of a fermentation culture medium are optimized, the production cycle can be greatly shortened, particularly, the effect of shortening the production cycle with high feeding concentration is remarkable and is obviously lower than that in the prior art, and therefore the production efficiency is greatly improved, and the production cost is reduced. And the time cost is reduced.

The invention discloses a method for synthesizing 11 alpha hydroxyprogesterone by using cytochrome P450 enzyme recombinant bacteria, and belongs to the technical field of biology. The method comprises the following steps: firstly, constructing CYP219A1 gene and reduction chaperone Pdx / Pdr gene expression vectors from neosphingosinearomaphilus, and transferring the CYP219A1 gene and reduction chaperone Pdx / Pdr gene expression vectors into escherichia coli to obtain whole-cell transformed recombinant bacteria; and after culture, feeding a substrate progesterone by using a resting cell method for biotransformation to obtain a 11 alpha-hydroxyprogesterone product. According to the CYP219A1 mutant strain provided by the invention, the conversion rate of progesterone is as high as 99%, and the 11 alpha site selectivity is 74%.

The invention discloses bifidobacterium animalis subsp. Animalis BN16 for stabilizing emotion and relieving anxiety and a metagen, the metagen is prepared by fermenting a bifidobacterium animalis subsp. Animalis BN16 and probiotic fermentation composition, and the composition is prepared from the following materials in parts by weight: 35 parts of milled rice with embryo, 30 parts of corn kernels, 17 parts of lotus seeds, 10 parts of walnut kernels and 8 parts of white granulated sugar. And 400 parts of purified water. The bifidobacterium animalis subsp. Animalis BN16 is fermented and then processed to prepare the metagen, nutritional ingredients in the metagen are directly, effectively and fully biotransformed, tryptophan absorption can be promoted, the situations of insufficient nutrition transformation, insufficient absorption and the like caused by poor intestinal digestion ability of people with sub-health emotions are avoided, emotion stabilization is facilitated, and the health-care effect is achieved. Anxiety is relieved, and the anxiety period is shortened.

This invention provides a method for preparing fermented pig feed based on bamboo powder and mycelium residue, belonging to the field of livestock and poultry feed preparation technology. Using bamboo powder and mycelium residue, a byproduct of edible fungi cultivation with bamboo powder as the core substrate, as raw material, the invention achieves efficient degradation of lignocellulose and bioconversion of nutrients in the bamboo powder and mycelium residue through core steps such as enzymatic pre-cell wall disruption pretreatment, segmented solid-state fermentation with compound functional microbial agents, and precise nutrient compounding. The resulting fermented pig feed is low in crude fiber, high in crude protein, palatable, and rich in bioactive substances. It can replace 10%-30% of traditional corn-soybean meal diets, significantly reducing feed costs for pig farming, improving pig growth performance and pork quality, and simultaneously constructing a closed-loop circular economy model of bamboo-fungi-livestock, possessing significant economic, ecological, and social benefits.