405results about How to "High biotransformation rate" patented technology

A process for puffing the culture medium of edible fungus, which is prepared from the twigs or the straw or stalk of agricultural crops includes such steps as pulverizing, proportionally mixing it with organic and inorganic auxiliaries, puffing and baking.

The invention provides a coprinus comatus cultivating method and a cultivating medium, which relates to cultivation of an edible mushroom in agriculture. In the coprinus comatus cultivating medium, driers of the cultivating medium is formed by blending 10-20% of cotton seed hulls, 60-80% of fungus chaff of needle mushroom and 8-25% of auxiliary materials, wherein the fungus chaff of the needle mushroom comprises the raw materials of 5-15% of wood powder and 10-30% of wheat stalks. The cultivating method is characterized in that by using the cultivating medium, the cost on cultivating the coprinus comatus is reduced, the residual nutrient contents in the fungus chaff of the needle mushroom are utilized completely, and the pollution of the fungus chaff of the needle mushroom to environment is avoided. The cultivating method is a low-carbon, economical and environment-friendly method for cultivating edible mushrooms, and the biotransformation efficiency can reach 116%.

The invention discloses a method for planting lentinus edodes by using mulberry branch wood chips as a planting material, mainly comprising the following steps: preparing the planting material; sterilizing the planting material at high temperature; inoculating and managing the output of the lentinus edodes; the method reasonably controls the material mixture ratio of the planting material through science and strictly controls technological conditions, such as temperature, humidity and the like in the planting process, therefore, the planted lentinus edodes has short growth period, high biotransformation rate, high yield, uniform individuals and abundant nutrition and belongs to a functional food of a novel generation.

The invention discloses a method for cultivating agaric with mulberry branches. The formula for cultivating agaric with mulberry branches consists of the following raw materials by weight: 80-85 parts of mulberry branch, 1-5 parts of rice bran, 1-5 parts of bran, 1-5 parts of corn meal, 1-5 parts of silkworm excrement, 1-5 parts of gypsum powder, 0.1-1 parts of lime powder, 1-3 parts of sugar, 1-5 parts of cottonseed meal, 1-3 parts of soybean powder and 0.1-1 parts of calcium carbonate. To cultivate agaric with mulberry branches has ample raw material sources, simple technology, low cost and quick effectiveness. Moreover, to cultivate agaric with mulberry branches can solve the contradictions between fungus and forest. To cultivate agaric with mulberry branches instead of forest logs not only has the effect of turning waste into treasure and improving the economic benefit of sericulture production, but also has great significance in the protection of the forest resource.

The invention relates to a Pholiota nameko cultivation medium. The Pholiota nameko cultivation medium is prepared by using the following raw materials, by weight, 30-40 parts of vine of ichosanthes Kirilowii Maxim, 30-40 parts of shell of Snakegourd Fruit, 20-30 parts of bagasse, 12-15 parts of sesame seed crumble, 6-8 parts of fecula, 5-6 parts of sesame seed meal, 8-10 parts of wheat bran, 2-4 parts of corn flour, 2-3 parts of sword bean powder, 8-10 parts of shrimp shell meal, 0.3-0.5 parts of calcium carbonate, 1-5 parts of quicklime, 1-2 parts of gypsum powder, 1-2 parts of plant ash, and 5-10 parts of a sustained-release nutritional particle. The Pholiota nameko cultivation medium has the advantages of wide raw material sources, and the healthcare efficacies of blood circulation invigorating, blood stasis dissolving and face nourishing of cultivated Pholiota nameko are increased.

The invention discloses a straw mushroom culture medium and a method for circularly cultivating straw mushrooms. The straw mushroom culture medium comprises 70 to 90 weight percent of Chinese medicament dreg, 10 to 30 weight percent of straws and a production increasing agent with the dose of 5 to 10 kg / m<3>, wherein the production increasing agent is prepared by mixing the following components in percentage by weight: 0.006 to 0.01 percent of vitamin B1, 15 to 20 percent of superphosphate, 40 to 60 percent of quick lime, 1.5 to 2 percent of magnesium sulfate, 0.2 to 0.4 percent of zinc sulfate, 0.2 to 0.3 percent of boric fertilizer, 0.2 to 0.4 percent of triacontanol and 22 to 37 percent of starch. By the straw mushroom culture medium and the method for circularly cultivating the straw mushrooms, resources can recycled; energy is saved due to short fermentation time of the medium; the medium can increase yield of the straw mushroom, can improve the quality of the straw mushroom and can increase the flavonoid content of the straw mushrooms; and by the multi-time circularly cultivating method, the utilization ratio of the medium is increased and production cost is reduced.

The invention relates to an edible fungus compost, a production method thereof and an edible fungus culture process. The edible fungus compost disclosed by the invention consists of 40 to 90 parts of residual waste (spent mushroom substrate) left over after the harvest of edible fungi, 5 to 40 parts of cottonseed hulls, 3 to 20 parts of bran, 2 to 8 parts of corn meal, 1 to 2 parts of gypsum powder, 0 to 5 parts of quicklime, 0 to 2 parts of superphosphate and 0.05 to 0.8 parts of fungus-strengthening substance, and the pH value is 6 to 9. The invention adopts the residual waste (spent mushroom substrate) left over after the harvest of edible fungi as main material ingredient to produce the edible fungus compost. By implementing the invention, the residual waste of edible fungi cannot pollute the environment any more, and is made profitable, and the resource can be recycled; an edible fungus material resource is added, the economic burden caused by the shortage and higher prices of edible fungus materials is decreased for edible fungus farmers, the production cost of the method is reduced by more than 50 percent in comparison with the production cost of the conventional method, the yield is increased by 10 to 15 percent, the bioconversion rate reaches 80 to 120 percent, and economic income and social benefit are increased. The method is applicable to a variety of edible fungi.

The invention provides a culture medium for cultivating coprinus comatus, which is composed of chestnut shells, crop straws or corncobs, dry livestock manure, calcium superphosphate, quick lime, enzyme bacterium and water. The method utilizing the culture medium to cultivate the coprinus comatus comprises the following steps: first, uniformly stirring all raw materials to produce a base material; second, stacking the base material for fermentation; third, bagging the fermented base material for inoculation so as to produce bacteria sticks; fourth, placing the bacteria sticks in a bacteria cultivating room; fifth, debagging the bacteria sticks so as to allow the bacteria sticks to be stood in a bed and is covered with soil; sixth, carrying out water spray management; and seventh, picking the coprinus comatus. According to the invention, the chestnut shells are basic material of the culture medium which is green and pollution-free, the cultivating process is simple, and the production cost is low; and the coprinus comatus is high in yield, good in quality, and high in biotransformation efficiency.

The invention relates to organic biological feed additives containing nano selenium and zinc oxide, a preparation method and a use method. The product of the organic biological feed additives is russety dry powder with a granularity between 80 and 140 nanometers, and can form suspension with water; the selenium content is between 0.00 and 36.00 percent and the zinc content is between 0.00 and 45.00 percent; the selenium content and the zinc content can not be 0 simultaneously; the organic biological feed additives are prepared by nano selenium organic biological intermediates and nano zinc oxide organic biological intermediates which are respectively prepared by fermentation of selenium dioxide and nano zinc oxide through microorganisms in the presence of various nutrient components; and 2.3 to 11.5 mass times of water is uniformly mixed with the organic biological feed additives containing the nano selenium and the zinc oxide and then sprinkled in feed which is then used for feeding laying hens, milk cows or nanny goats for 5 to 10 days. Compared with contrast feed, the selenium content and the zinc content of produced eggs are respectively 2 to 5 times and 2 to 2.5 times; the selenium content and the zinc content of produced milk are respectively 3 to 6 times and 2 to 4 times; and the selenium content and the zinc content of produced goat milk are respectively 3 to 7 times and 3 to 6.5 times.

The invention relates to edible fungus compost taking pepper stalks as main materials and application of the compost, and belongs to the technical field of edible fungus cultivation. The main components of the edible fungus compost are the pepper stalks in an amount which are 60 to 87 percent of the mass of the edible fungus compost; and bran, sugar, urea, corn meal, cottonseed hull, calcium superphosphate, a cake fertilizer, lime or / and gypsum powder and the like can be used as auxiliary materials. The invention also provides a bag cultivation method, a fermentation cultivation method, a clinker bag cultivation method and the like for cultivating edible fungi. Mushrooms are cultivated by taking the pepper stalks as the main materials, so that the utilization rate of the pepper stalks is improved, and waste is turned into wealth; and an appropriate quantity of auxiliary materials are added into the ground pepper stalks, so that a carbon-nitrogen ratio suitable for the growth of the mushrooms is reached, and the compost has the characteristics of low fungus input, multi-cropping cultivation and harvest and high biological conversion rate in the aspect of planting the mushrooms.

The invention discloses a method of producing a protein feed by a liquid-solid two-step fermentation method. The method comprises the following steps: performing liquid culture on corynebacterium glutamicum, candida utilis, bacillus subtilis, lactobacillus plantarum and aspergillus oryzae so as to obtain corynebacterium glutamicum fermentation liquor, candida utilis fermentation liquor, bacillus subtilis fermentation liquor, lactobacillus plantarum fermentation liquor and aspergillus oryzae fermentation liquor; mixing the corynebacterium glutamicum fermentation liquor, the candida utilis fermentation liquor, the bacillus subtilis fermentation liquor, the lactobacillus plantarum fermentation liquor and the aspergillus oryzae fermentation liquor so as to obtain mixed bacterium liquor; loading raw materials of soybean meal, cluster bean meal, cottonseed meal and the like into an explosion vat for performing steam explosion; after steam explosion, mixing materials, the mixed bacterium liquor and molasses in proportion, and adding an enzyme preparation so as to obtain mixed materials; conveying the mixed materials into a special modularized solid-state biological raw material continuous fermentation device for enzymolysis and fermentation, and drying the fermented materials so as to obtain the protein feed. According to the method disclosed by the invention, the modularized automatic fermentation device is adopted, the materials after explosion, the fermented bacterium liquor and the enzyme preparation are mixed, then the mixed materials are loaded into the automatic fermentation device for enzymolysis and biological fermentation, so that requirements for automatic temperature control, automatic dampness control and automatic ventilation control are met, the bioconversion rate is high, the fermentation period is short, the consumption is low, the cost is low, and mechanized and large-scale production requirements can be met.

The invention relates to a coprinus comatus culture base material prepared from the following raw materials in parts by weight: 30-40 parts of corncob particles, 20-30 parts of erianthus arundinaceus powder, 20-25 parts of food factory leftovers, 30-40 parts of cotton stalk particles, 10-12 parts of peanut cakes, 4-6 parts of onion powder, 2-3 parts of calcium superphosphate, 1-2 parts of quicklime, and 5-10 parts of slow-release nutrition particles. As the slow-release nutrition particles are added into the culture material, coprinus comatus cultured by the base material is high in bioconversion rate and rich in nutrition, the healthcare effect of reducing three highs is improved at the same time, and the coprinus comatus eaten frequently can achieve an effect of prolonging life.

The invention discloses a large-field high-yield cultivation method for stropharia rugoso-annulata, which comprises the following steps: a, manufacturing a cultivation material; b, selecting a cultivation place and setting a seeding area in the cultivation place; c, setting a cultivation layer in the seeding area and seeding strains; d, vertically covering a soil layer and a straw layer to the seeded cultivation material layer; and e, preserving heat and humidity for the cultivation material layer till mushrooms grow out. The large-field high-yield cultivation method for the stropharia rugoso-annulata directly carries out stockpiling treatment and strain cultivation in a large field under the outdoor condition, strictly and scientifically controls the process conditions of material proportion, temperature and the like during the cultivation, and has little pollution and low cost; and the cultivated stropharia rugoso-annulata has high biological conversion rate, short growth period, high hypha survival rate, large and even seed individuals, good quality and enough mushroom delayed effect.

The invention discloses a culture material for industrial cultivation of Pleurotus eryngii. The culture material is made from the following raw materials by weight: 17 parts of white poplar twood chips, 17 parts of corn cobs, 10 parts of Jujun grass or elephant grass, 10 parts of bagasse, 20 parts of wheat bran, 10 parts of soybean meal, 10 parts of corn flour, 5 parts of light calcium carbonate and 1 part of lime. The Pleurotus eryngii product cultivated by the culture material provided by the invention is detected to meet the requirements of green fool, the fruiting speed is fast, and the fruiting cycle is shortened. A cycle lasts for 46 days, wherein spawn running accounts for 26 days, and the fruiting accounts for 20 days. The product quality is good, and the biological conversion rate is high and is up to over 70%. The finished product mushroom has a high proportion, which accounts for more than 85% of the yield.

The invention discloses a composite microbial preparation as well as a preparation method and an application thereof. The composite microbial preparation comprises Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae. The composite microbial preparation is used for fermentation process of microbial silage forage, and can be used for effectively improving fiber degradation rate and biotransformation rate of the forage, improving NDF digestibility, increasing crude protein content, inhibiting breeding of harmful bacterium, and shortening fermentation period with wide application prospects and market values.

The invention discloses a glutathione producing strain, in which a GSH related synthetic enzyme Gamma-L-glutamyl-L-cysteine synthetic enzyme gene GSH I, a glutathione synthetic enzyme gene GSH II, and a cysteine related synthetic enzyme cystathionine synthetic enzyme gene CYS3 and a cystathionine-Gamma-lyase CYS4 gene are introduced. The glutathione producing strain of the invention has the advantages of high density and low cost.

The invention discloses a method of cultivating stropharia mushrooms by using ramie stalks. The method comprises the following steps that the ramie stalks are collected and dried in the sun; the ramie stalks are soaked; the ramie stalks are drained naturally, wheat bran and unslaked lime are added to the ramie stalks, mixed evenly and piled up, and fermentation is performed; compost is flatly laid on a fungus bed cultivation field, flattened and compacted, three layers of the compost are laid, and fungus seeds are sowed; soil covering and fruiting management are performed; pest control is performed; when gills of fruiting bodies are not fractured or are just fractured and caps are in a bell shape, the stropharia mushrooms are in the optimum period of harvest, and after the stropharia mushrooms are picked, holes left in fungus beds need to be filled up flatly, and remaining mushrooms need to be cleared away. The method of cultivating the stropharia mushrooms by using the ramie stalks makes full use of the ramie stalks, improves comparative benefits of ramie farmers, stabilizes the ramie planting area, integrates biological characteristics of the stropharia mushrooms, lowers labor cost, improves product quality and biotransformation efficiency of the stropharia mushrooms, and improves the yield. The method provides a new way of development and utilization of the ramie stalks, reduces pollution to the environment caused by incineration of the ramie stalks, makes use of waste, and achieves two things at one stroke.

The invention discloses a high-yield bagged material culture method of tea tree mushrooms, comprising the following steps of bagged material making, inoculation, mycelium culture and fruiting body culture. Bagged material has the advantages of scientific material matching and reasonable ratio and can supply essential nutrition to myceliums and fruiting bodies. The technological conditions of temperature, humidity and the like are controlled strictly in the culture process to accelerate mycelium growth and fruiting body primordium differentiation; thus, the invention has the advantages of good fruiting body development, high biological transformation rate, little misshapen mushroom production, uniform individual fruiting body, high quality, long mushroom production period, high yield, little pollution and low cost and further enhances the utilization ratio of cultural materials.

The invention discloses a corn straw fermentation microorganism agent which consists of phanerochaete chrysosporium, trichoderma koningii, bacillus subtilis, bacillus licheniformis and monilia tropicalis. The corn straw fermentation microorganism agent disclosed by the invention has the beneficial effects that the microorganism agent is reasonable in formula and convenient to use; the straw rotting speed is high; the corn straw fermentation microorganism agent has the characteristics of new combination, stable structure, high enzyme activity, high microbial content and the like. The corn straw fermentation microorganism agent can be used for performing high-efficiency and environment-friendly fermentation on corn straw materials, so that a feed can contain a large amount of microorganism live cells, clastic enzymes and other active components, and the utilization rate of the corn straw materials can be effectively increased. The practical significance of solving the problem of environment pollution is achieved; furthermore, the actual application values such as the protein content and the nutritional value in the corn straw materials can be improved; good preconditions are created for recycling application of corn straws.

The invention relates to a shiitake cultivation medium. The shiitake cultivation medium is prepared through using the following raw materials, by weight, 30-40 parts of a bean straw particle, 30-40 parts of a Corchorus capsularis L stalk particle, 20-30 parts of husk, 10-20 parts of sugarcane leaf powder, 10-15 parts of walnut shell flour, 15-16 parts of corn flour, 8-10 parts of vinegar residue, 10-12 parts of bean dregs, 4-5 parts of Phaseolus radiatus L., 8-10 parts of fishbone meal, 1-2 parts of lactose, 0.3-0.5 parts of calcium carbonate, 2-3 parts of quicklime, 1-2 parts of gypsum powder, 0.5-1.0 part of calcium superphosphate, 0.2-0.3 parts of potassium dihydrogen phosphate and 5-10 parts of a sustained-release nutritional particle. The shiitake cultivation medium has the advantages of wide raw material sources, increase of the heat clearing and exterior syndrome relieving effects of shiitake cultivated through the medium, immunity improvement, and low raw material cost.

The invention provides a bamboo fungus cultivation method. The raw material formula and the processes are as follows: (1) the raw material formula comprises 500 kilograms of broad-leaved tree wood chips, crop straw or a corncob smashing material, 100 kilograms of bran, rice bran or corn flour, 1.5 kilograms of compound fertilizer, 0.8 kilogram of carbamide, 8 kilograms of lime powder, 4 kilograms of land plaster, 0.6 kilogram of potassium dihydrogen phosphate, 5 kilograms of white sugar and 330-350 kilograms of water; adding 3-5 kilograms of a 'Gymboree edible mushroom leavening agent' produced by the Beijing China Healthhead Science & Technology Co., Ltd; (2) fermenting; (3) conducting sterilization on a fermented material; (4) performing inoculation and cultivation; (5) performing cultivation in workshops or in rural cooperatives. By the skillful application of the biological fermentation technology, fundamental innovation and improvement of the zhijin bamboo fungus raw material and the seed production and cultivation technology are realized, so that the production cost is decreased by more than 50%; the biotransformation rate of the raw material to fresh zhijin bamboo fungus reaches more than 100:50, so that the key technical problem of industrialized cultivation of zhijin bamboo fungus is solved and the market need is fulfilled.

The invention relates to a cultivation method of imitated wild ganoderma lucidum in a cedar forest. The method comprises the following steps: (1) selecting a strain; (2) preparing mushroom-sticks; (3) carrying out cultivation and management; (4) controlling diseases and pests. After the method is adopted, the defects that the existing greenhouse ganoderma lucidum cultivation technology is not scientific and reasonable enough in formula of a culture medium, and is low in content of effective components of sporocarp, low in quality and high in input cost, pollution by infectious microbe is easily caused, and the like can be overcome.

The invention relates to the field of novel cultivation techniques of edible fungi, in particular to a cultivation method of large Ganoderma lucidum. The method includes the steps of using broad-leaved wood 6-30cm in diameter as raw material in October to November each year, sawing the wood into short segments, chopping the short segments into irregular wood blocks (sheets), bagging, inoculating, and cultivating fungus cylinders; performing earthing cultivation on the fungus cylinders in a light environment which can be 70% shady and 30% shinny in March of the next year; setting 15 fungus cylinders in a group during cultivation, and cultivating one mushroom of large Ganoderma lucidum with cap diameter up to 80-120cm by all mycelia and nutrients in the 15 fungus cylinders; after the first mushroom of large Ganoderma lucidum is picked, cultivating a second mushroom of large Ganoderma lucidum with the cap diameter up to 40-60cm in the same management way as the first mushroom of large Ganoderma lucidum. Through the application of the method, fruiting bodies of the cultivated large Ganoderma lucidum are large and magnificent with high quality and benefits produced are extraordinarily significant.

The invention provides a standardized green planting method of selenium-rich peppers and relates to the technical field of crop planting. The standardized green planting method of the selenium-rich peppers comprises the following steps of 1 intensive soil preparation, 2 variety selection, 3 rational close planting, 4 intertillage strengthening, 5 timely field ponding discharging, 6 formulated fertilization, 7 chemical regulation and control, 8 comprehensive insect disease prevention and control, 9 timely harvesting and the like. The problem that at present, peppers produced by adopting a traditional peppers cultivation technique are low in selenium content is effectively solved. By means of the technical scheme, the organic selenium content in the peppers is greatly improved, the organic selenium of the peppers cultivated by means of the standardized green planting method is easily absorbed by a human body, and the standardized green planting method has the advantages of being safe in eating and high in biotransformation rate.

The invention relates to a black fungus cultivation base material. The black fungus cultivation base material is prepared by using the following raw materials, by weight, 30-60 parts of leftover chip from a wood working factory, 20-30 parts of a jute stalk particle, 10-15 parts of reed powder, 8-10 parts of soybean meal, 10-12 pars of broken rice, 0.5-0.8 parts of calcium carbonate, 1-2 parts of quicklime, 0.5-0.8 parts of calcium superphosphate and 5-10 parts of a sustained-release nutrition particle. The raw materials of the black fungus cultivation base material have wide sources, and black fungi cultivated through using the base material have the healthcare efficacies of heat clearing, detoxifying, mind tranquillizing and pressure reducing.

A high-yield cultivation method for selenium-enriched peppers includes the following steps of: land selection; variety selection; seed treatment; nutrient soil preparation; sowing; planting and transplanting; flower period management of the selenium-enriched peppers; and pepper harvesting. The step of seed treatment is characterized in that: selected seeds are put into an organic selenium solution to soak for 6 to 12 hours, wherein the temperature of the organic selenium solution is 25 to 30 DEG C, and the seeds can absorb a part of organic elements after being soaked in the organic selenium solution, so that the seed nutrients can be improved, the germination rate of the seeds can be increased, and diseases and pests can be prevented; the seeds are washed through clear water, and then are soaked in warm water being at the temperature of 30 DEG C for 10 to 12 hours, the seeds are put into a germination box to germinate, the temperature during germination is controlled to be 28 to 35 DEG C, and the seeds are sown when 70% of the seeds germinate. The step of flower period management of the selenium-enriched peppers is characterized in that: during a flower period of the selenium-enriched peppers, the organic selenium solution is sprayed into leaves of the selenium-enriched peppers for 2 to 3 times, the amount of the organic selenium solution is 10 to 15 Kg per mu, and a time interval between the adjacent two fertilizations is 10 to 12 days. According to the high-yield cultivation method for the selenium-enriched peppers, the content of organic selenium in the planted peppers can reach 4.0 mg / Kg; and compared with the yield of the conventional cultivation method for the selenium-enriched peppers, the yield of the selenium-enriched peppers is increased by greater than 18.3%.

The present invention provides one method of using methanol utilizing yeast, especially transferred recombinant saccharomycete with ectogenic S-adenosyl-menthionine (SAM) synthetase expression vector, to produce SAM and the method of constituting the expression vector and transferred cell. The recombinant saccharomycete is used in fermentation of culture medium with methanol, proper amount of methionine and certain nitrogen source, high yield of SAM may be obtain and SAM content in cell may reach 10-20 % of dry cell weight. The present invention also provides one methanol utilizing Pachia pastoris sacchromycete strain with preserving number of CGMCC No.0648.

The present invention relates to high quality edible fungus culturing material and its production process and edible fungus cultivating process. The edible fungus culturing material is compounded with organic matter containing cellulose and lignin, auxiliary nitrogen source, long acting nitrogen source, quick acting nitrogen source and water, and through natural fermentation and cooling to room temperature. The present invention has wide edible fungus culturing material source, low edible fungus producing cost,shortened edible fungus producing period and high edible fungus yield. The present invention is suitable for all kinds of edible fungus.

The invention relates to a hericium erinaceus culture base material prepared from the following raw materials in parts by weight: 30-50 parts of rape straw particles, 30-40 parts of broomcorn straw particles, 10-15 parts of lotus seed shell particles, 16-18 parts of wheat bran, 8-10 parts of overdue milk powder, 4-6 parts of pepper seeds, 8-10 parts of eggshell meal, 0.3-0.5 part of calcium carbonate, 1-5 parts of quicklime and 5-10 parts of slow-release nutrition particles. Hericium erinaceus cultured by the culture base material is high in bioconversion rate and rich in nutrition, the healthcare effect of dispersing the liver depression is improved at the same time, and the hericium erinaceus eaten for a long time has healthcare effects of dispersing the liver depression and reducing the disease incidence of cardiovascular and cerebrovascular diseases.