43 results about "Insect disease" patented technology

The invention relates to the technical field of disease and insect pest detection, and further relates to a disease and insect pest image multispectral imaging and deep learning intelligent detection system, which comprises a multispectral radiance calibration unit, an image analysis and processing unit and a disease and insect pest probability detection unit, the multispectral radiance calibration unit is used for carrying out multiband imaging on vegetation leaf surfaces in the forest region, deducting dark field digital counts from original digital counts and then converting the original digital counts into radiance of corresponding bands; the image analyzing and processing unit is used for converting the radiance into surface feature apparent reflectivity; multiplying the scab spectrum gradient contrast index by the spectrum heterogeneity index to obtain coupled pest and disease damage characteristics; and the pest and disease damage probability detection unit is used for mapping the convolution output of each layer into a pest and disease damage probability value based on a generalized error function. The accuracy and practicability of disease and pest detection are remarkably improved.

The invention discloses a citrus orchard interplanting pest and disease prevention and control method, which comprises the following steps: installing a drip irrigation facility in a citrus orchard, and uniformly distributing drippers of the drip irrigation facility between each citrus tree and green manure planting rows; soil humidity sensors are buried in multiple positions of the citrus orchard to monitor soil humidity data in real time through the soil humidity sensors; and determining whether to start the drip irrigation facility based on the soil humidity data acquired by the plurality of soil humidity sensors and the position relationship among the plurality of soil humidity sensors. Therefore, whether the irrigation facility is started or not can be automatically decided by monitoring the soil humidity of different positions in the citrus garden in real time, and the intelligent control mode is beneficial to timely discovering and processing the soil water shortage condition; the problems that in a traditional mode, whether irrigation is carried out or not cannot be judged according to the actual humidity condition of soil, and irrigation hysteresis exists are solved, and then a basis is provided for citrus orchard interplanting pest and disease prevention and control management.

The invention provides a plant insect disease image identification method and system based on deep learning, and belongs to the technical field of deep learning, and the method comprises the steps: firstly obtaining a multi-source image set of a plurality of growth stages of a plant, including the whole, leaf and stem images of the plant, the growth stage time, and the shooting angle identification information; constructing a plant image time sequence association network to perform organ-level time sequence alignment processing to obtain an aligned image set and an organ association coefficient, performing cross-growth-stage focus feature association through the association coefficient to obtain a cross-stage focus association set and time sequence evolution information, and performing cross-growth-stage focus feature association; and inputting the association set into an exclusive deep learning model for feature aggregation and type matching to obtain a preliminary result, and finally performing organ adaptation verification on the preliminary result in combination with time sequence evolution information to generate a final identification result and a focus organ time sequence evolution atlas, thereby improving the accuracy and reliability of plant insect disease identification.

Proposed are recombinant entomopathogenic bacteria transformed using promoter replacement technique, a preparation method, and uses thereof for producing pesticides and controlling pests. Provided are recombinant entomopathogenic bacteria in which the promoter of the GXP synthase gene (gxpS gene) is replaced in the entomopathogenic bacteria with an arabinose inducible promoter using a promoter replacement technique. The recombinant entomopathogenic bacteria prepared in the present disclosure can produce large quantities of GXPs, which acts to suppress immunity of pests, by controlling the expression of the GXP synthetase gene (gxpS gene) through an addition of an inducer. The recombinant entomopathogenic bacteria prepared in the present disclosure can also effectively control pests through a response that suppresses the immunity of pests.

A formulation containing an attractant specific for a targeted pest insect and an entomopathogenic bio-insecticide to control pest insect populations. By attracting the insect pest into a trapping device, loading it with the formulation and then, at exiting the trapping device, the insect will disseminate in the field where it will eventually die. Wherever some formulated material will have been distributed in the field, further fellow creatures of the same species will be attracted and infected, thus multiplying the effect of the primary trapping device. The pest insect is used as the vector of its own demise, effectively using a minimal amount and limited applications of pesticides of biological origin.

The present invention relates to microencapsulated preparations comprising biopesticide, bio-fungicide, and plant growth promoting agents and uses thereof, as well as to methods for controlling pests and for producing said preparations for managing insect pests. In particular, the invention relates to microencapsulated preparations comprising at least one insect-pathogenic (entomopathogenic) fungus selected from Metarhizium spp. and Beauveria bassiana, and at least one pathogenic Bacillus spp. bacterial strain, preparations and particles comprising these, their use on plant material, methods for controlling pests therewith, and processes for their production.

Provided herein is a composition comprising a Pickering emulsion comprising a plurality of core-shell particles encapsulating an entomopathogenic organism. Furthermore, a method for controlling a pest on or within a plant or at the area under cultivation is provided.

Novel strains of the insect-pathogenic fungus, Metarhizium var. anisopliae fungal strain BNL 101 deposited in the CABI UK Centre, United Kingdom, having IMI CC Number 506833; or BNL 102 deposited in the CABI UK Centre, United Kingdom, having IMI CC Number 506834; or a culture having the identifying characteristics thereof, are disclosed. The present invention also discloses methods of using the fungal strains, and spores obtained therefrom, to control insects, and provides a natural pest control preparation. The preparations and compositions comprise several unique and desirable features, such as a wide host range or alternatively a selective host range, and a consistent pathogenicity. The preparations and compositions have a high virulence (insecticidal activity), that is 2 times to 3 times, or greater, mor virulent (insecticidal) than compositions or preparations that do not contain the preparation or composition, and especially that do not contain the BNL 101 or BNL 102 fungal strain, or spores therefrom. The fungal strains also provide for a high spore yield in production, and possess a high stability in the field.

The present disclosure relates to a Beauveria bassiana KNU-101 strain. The Beauveria bassiana KNU-101 strain is entomopathogenic, exhibits an excellent insecticidal effect on controlling thrips, which are soil pests that harm crops, and also has excellent sporulation ability. Thus, the Beauveria bassiana KNU-101 strain may be effectively used as an environmentally friendly means for controlling pests.

The invention provides a secretory protein with insecticidal activity and an application of the secretory protein in pest control, an entomopathogenic fungus beauveria bassiana is used as a research material, and the secretory protein BbTsp1 is identified by using a protein spectrum technology. The protein is prepared and purified by using a prokaryotic expression system. A host blood cavity injection experiment proves that the protein has the activity of killing a host. Through a gene recombination technology, the BbTsp1 gene is introduced into beauveria bassiana, and an overexpression strain of the gene is constructed. Bioassay shows that the insecticidal activity of the strain is remarkably improved. The construction of a recombinant strain of the toxicity related gene identified by the invention improves the pest control efficiency of insecticidal fungi.

The present disclosure provides an entomopathogenic fungi conidia powder, which comprises a powder and conidia attached to the surface of the powder. On the other hand, the present disclosure further provides a method for producing the entomopathogenic fungi conidia powder. Based on the disclosure of the present disclosure, the production method uses a powder to adhere to the entomopathogenic fungi conidia, and allowing them to be stored at room temperature for at least 7 months with a substantially same amount of conidia population. In addition, the production method using solid fermentation reduces production costs.

The invention relates to the technical field of functional fertilizers, and discloses a heavy metal and insect disease double-effect regulation type organic fertilizer and a preparation process thereof, the fertilizer is a particle with a three-layer structure of core-inner layer coating-outer layer coating; the core is prepared from a composite passivation functional body, an organic nutrition matrix and a binder; the composite passivation functional body is a flavone-modified functional biological porous carrier loaded with strontium ion-doped nano-hydroxyapatite, and is used for fixing soil heavy metals and regulating and controlling rhizosphere micro-ecology; the inner-layer coating is prepared from wood vinegar, the outer-layer coating is prepared from chitosan oligosaccharide and sodium alginate, and the layered structure realizes sequential release of immune induction substances and synergistically induces plant resistance. Through specific structural design and component synergy, triple effects of heavy metal fixation, rhizosphere micro-ecological regulation and control and plant disease and insect pest induced resistance are achieved, and the effect is definite.

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

The utility model relates to the technical field of citrus reiculata Blanco insect prevention spraying devices, in particular to a citrus reiculata Blanco planting insect disease prevention spraying device which comprises a water storage barrel, a spraying device is arranged on one side of the water storage barrel, the spraying device comprises a booster pump arranged on one side of the water storage barrel, and the water inlet end of the booster pump is communicated with the water storage barrel through a suction pipe. A conveying pipe is fixedly installed at the water outlet end of the booster pump, a plurality of hoses are fixedly installed on the conveying pipe, an erecting pipe is arranged on one side of each hose, an end pipe is fixedly installed on a pipe body at the bottom end of the erecting pipe, the hoses are connected with the end pipes through flanges, a horizontal pipe is fixedly installed on a pipe body at the top end of the erecting pipe, and a hard pipe is fixedly installed on the horizontal pipe. A plurality of uniform distribution pipes are fixedly mounted on the hard pipe, a plurality of spray heads are fixedly mounted at the bottoms of the uniform distribution pipes, the hard pipe and the horizontal pipe, and a supporting disc is fixedly mounted at the bottom end of the erecting pipe. The spray deinsectization device facilitates spray deinsectization operation and is convenient to use.

This invention discloses an insect pathogen composition for controlling tomato leafminer, its preparation method, and its application, belonging to the field of agricultural technology. The insect pathogen composition for controlling tomato leafminer of this invention includes a compound agent of symbiotic bacterial fermentation products of insect pathogenic nematodes sprayed on tomato leaves, a compound preparation of insect pathogenic nematodes applied in holes during tomato seedling transplanting, and a simulated tomato leaf-bearing moth-attracting ball for trapping tomato leafminer. This invention also discloses the preparation method of this insect pathogen composition for controlling tomato leafminer and its application in controlling tomato leafminer. This invention can reduce the adult insect population density in greenhouses; reduce the opportunity for female tomato leafminer to lay eggs on leaves and fruits, and reduce the overall egg production in greenhouses, thus reducing the larval population density. This invention achieves its control of tomato leafminer through the synergistic effect of multiple components.

The invention discloses an entomopathogenic composition for preventing and treating tomato leaf miners as well as a preparation method and application of the entomopathogenic composition, and belongs to the technical field of agriculture. The entomopathogenic composition for preventing and treating the tomato leaf miner comprises a symbiotic bacterium fermentation product compound agent of entomopathogenic nematodes sprayed on tomato leaves, a compound preparation of the entomopathogenic nematodes applied in holes when tomato seedlings are transplanted, and simulated leaf tomato moth trapping balls for trapping the tomato leaf miner. The invention further discloses a preparation method of the entomopathogenic composition for preventing and treating the tomato leaf miner and application of the entomopathogenic composition in prevention and treatment of the tomato leaf miner. The population density of imagoes in the greenhouse can be reduced; the chance that female tomato leaf miners lay eggs on leaves and fruits is reduced, the total egg laying amount in a shed is reduced, and the population density of larvae is reduced. Through the synergistic effect of a plurality of components, the effect of preventing and treating tomato leaf miners is achieved together.

The application discloses an insect pathogenic preparation for preventing and treating Cylas formicarius, a preparation method and application thereof, and belongs to the field of agricultural technology. The insect pathogenic preparation for preventing and treating Cylas formicarius comprises a corpse compound agent of an insect pathogenic nematode and a sweet potato root protection agent. The corpse compound agent of the insect pathogenic nematode comprises an insect pathogenic nematode corpse agent, an artificial feed of Cylas formicarius, a polyphenol oxidase inhibitor and trehalose (alpha,alpha) dry powder, and the mass ratio is 1-3:6-8:0.25-0.35:0.6-0.8. Each 1L of the sweet potato root protection agent contains 0.03-0.05g of a biocontrol bacterium agent, 3-5g of a Cylas formicarius repellent and 90-110ml of a bonding agent, and the volume is supplemented with water. The application further discloses a preparation method of the insect pathogenic preparation and application thereof in preventing and treating Cylas formicarius. The insect pathogenic preparation can prevent and treat Cylas formicarius through multiple actions, and has a good prevention and treatment effect on adult and larva of Cylas formicarius.

The invention relates to the technical field of asparagus lettuce cultivation, in particular to an asparagus lettuce high-yield cultivation method which comprises the following steps of seedbed preparation, seed treatment, seedling management, field planting, field management and harvesting. In the seedling raising seedbed preparation period, peat of vermiculite is added into an added fertilizer, the air permeability, the water-retaining property and the emergence rate of seeds can be improved, the fresh weight of seedlings can be improved, trichoderma harzianum powder and brown sugar water are added in the seed treatment process, the seeds can be wrapped by flora through trichoderma harzianum, the seeds can be wrapped by the flora through trichoderma harzianum, and the seedling raising effect is improved. The ammonia water fertilizer added in the seedling stage management period can enable the asparagus lettuce to stably grow and prevent the root from being burnt out due to excessive growth, stem cracking can be prevented, the toughness of the epidermis can be improved, the insect disease disaster of the asparagus lettuce can be reduced, and the yield value per mu of the asparagus lettuce can be increased.

The invention discloses an insect disease prevention and control tree trunk punching and pesticide injection tool, and belongs to the technical field of insect disease prevention and control. The device comprises a body and a fixing block, the fixing block is rotationally connected with a fixing pipe in a penetrating mode, the fixing pipe is rotationally connected with a conveying hose, the conveying hose is rotationally connected with the body, the fixing pipe is provided with a plurality of medicine injection holes, the fixing block is provided with a clamping assembly, and the fixing pipe is rotationally connected with a fixing rod. The fixing rod is fixedly connected with a threaded drill bit, the fixing pipe is provided with a control assembly, the control assembly comprises a threaded cylinder slidably connected to the outer portion of the fixing pipe in a sleeving mode, and the threaded cylinder is in threaded connection with a control panel. When punching and medicine injection operation needs to be carried out, after the whole device is moved to a proper punching position, a motor is started, punching is started, after punching operation is completed, medicine injection operation can be automatically carried out, a threaded drill bit does not need to be moved, the whole work is automatically carried out, and the working efficiency is high.

The invention provides a pine wood nematode disease identification method and device based on the inspection view angle of an unmanned aerial vehicle and a medium, and belongs to the field of forestry disease monitoring and prevention, and the method comprises the steps: setting the height, flight speed and shooting angle of the unmanned aerial vehicle, and obtaining an image shot by the unmanned aerial vehicle in the inspection process in a preset region; inputting the image into an insect disease detection model to obtain an insect disease detection result; wherein the insect disease detection model is obtained by training in a mode of adjusting model parameters by adopting a stochastic gradient descent optimizer and a complete intersection-to-union ratio loss function. According to the identification method provided by the embodiment of the invention, data preprocessing is performed on the picture shot by the unmanned aerial vehicle, the pine wood nematode disease is automatically identified by using the deep learning algorithm, and manual field detection is replaced by automatic identification, so that the problems that manual inspection consumes a large amount of manpower and is influenced by the environment are avoided, the manpower resource waste is reduced, and the detection efficiency is improved.

Disclosed are methods for increasing infectivity of entomopathogenic nematodes in any target area for the purposes of insect control, said method comprising:(a) mixing an aqueous nematode infectivity increasing composition with (i) entomopathogenic nematodes to activate said entomopathogenic nematodes for increased infectivity prior to application of said entomopathogenic nematodes to said target area, or (ii) seeds to produce coated seeds, and(b) applying said entomopathogenic nematodes to said target area or planting said coated seeds in said target area;wherein said aqueous nematode infectivity increasing composition is produced by methods disclosed herein.

The invention provides application of beauveria bassiana HFBb18 in preparation of a green peach aphid biological control entomopathogenic fungus agent, and belongs to the technical field of agricultural biology. The inventor acquires and identifies three new strains of beauveria bassiana from the field, then selects three strains of beauveria bassiana preserved in a laboratory, and finds that the newly found HFBb18 strain has high toxicity to green peach aphids through indoor toxicity determination. On the basis, the influence of infection of the HFBb18 strain on the activities of detoxifying enzyme and protecting enzyme of green peach aphid is determined. Furthermore, the wettable powder taking the HFBb18 strain conidia as a main active ingredient is prepared, the potting pesticide effect test control effect of the wettable powder on green peach aphids is defined, and a foundation is laid for development and application of a novel fungal aphid killing agent.

The utility model discloses an insect trapping device for preventing insect diseases and insect pests for tea planting, which belongs to the technical field of tea planting and is characterized by comprising an insect trapping box, an insect killing mechanism is fixedly connected to the top of the insect trapping box, a control mechanism is fixedly connected to the inner side of the insect trapping box, and a collecting box is slidably connected to the bottom of the inner side of the insect trapping box. The pest killing mechanism is provided with an ultraviolet light trap lamp and a blue light trap lamp, light with different wavelengths can attract various pests with different habits and preferences of different spectrums, the pest trapping range is expanded, attraction to various pests in a tea garden is increased, accordingly, the number of the pests is more effectively reduced, it is guaranteed that tea planting is prevented from being invaded and disturbed by the pests, and the tea planting quality is improved. The control mechanism monitors the humidity condition in the insect trapping box in real time, when the humidity exceeds a suitable range, the ventilation exhaust fan is controlled to work, internal and external exchange of air is achieved through the ventilation holes formed in the two sides of the rear side of the insect trapping box, the humidity in the box is adjusted, and the humidity in the box is kept at the level beneficial to insect trapping and normal operation of equipment.

By the present invention it has now been found that certain mixtures of 4-(trifluoromethylnicotinamide) and active compound compositions comprising such mixtures are suitable for controlling nuisance insects, disease-carrying insects or hematophagous (blood-feeding) insects, parasites including diptera, triatomine mosquitoes and parasitic insects of the genus kimicidus by expression suppression or by inhibition of blood feeding. In one embodiment, the diptera parasites are selected from flies, gnats and mosquitoes, including insecticide-resistant flies, gnats and mosquitoes, as well as flies, gnats and mosquitoes carrying pathogenic disease. The target parasitic insects of the genus kimicid are selected from bed mites. The target parasites of the genus kimicid are selected from kissing mites. Other aspects of the present invention will also be apparent from the detailed description that follows.

The invention discloses a praziquantel derivative PT-28 as well as a preparation method and application thereof. According to the praziquantel derivative PT-28, a thymol precursor and amino-praziquantel react to generate the derivative. The praziquantel derivative PT-28 can be used as a drug for resisting third-generation insect diseases, and compared with the national standard anti-parasitic drug praziquantel, the safety index-therapeutic index (TI value) of the drug is improved by 8.89 times. The compound has the characteristics of being safe to fishes, low in toxicity and good in killing effect on third-generation insects, and has wide application prospects in development of novel insect-resistant fishery drugs.

The present disclosure relates to insect pest control. Particularly, the present disclosure relates to a method of controlling insect pests with an entomopathogenic fungus. More particularly, the present disclosure relates to a method of controlling insect pests with strains of Beauveria bassiana or their spores. Also provided herein, is the composition comprising said strains of Beauveria bassiana or their spores and use of said composition for controlling or preventing insect pests.

The present invention provides stable, non-aqueous biopesticides. The present invention relates to biopestic formulations of entomopathogenic fungi or their spores. In particular, the present invention discloses oil dispersions containing entomopathogenic fungi or their spores, especially Beauveria bassiana. The present invention also relates to methods for preparing the formulations and their use.

The invention discloses a Beauveria bassiana strain and a method for controlling grassland caterpillars thereof, and relates to the field of insect pathogenic microorganism control. The Beauveria bassiana GC23620 strain of the present invention is isolated from the body surface of grassland caterpillar larvae and is classified and named Beauveria bassiana. Beauveria bassiana , the deposited name is Beauveria bassiana ( Beauveria bassiana ) strain GC23620, deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, the address of the deposit unit is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, the deposit date is January 29, 2024, the strain deposit number is CGMCC: NO.41056, the above-mentioned Beauveria bassiana GC23620 strain has high pathogenicity to grassland caterpillar larvae and significant insecticidal effect. This strain is an insect pathogenic fungus and can be used as a living biological pesticide; it is safe and efficient during the application process, environmentally friendly, pollution-free and residue-free, and has very strong application potential in the biological control of grassland caterpillars.

The present invention is related in general, to the field of biotechnology, especially to the use of genetic engineering in the development of entomopathogenic fungus with desirable traits to increase efficiency in the biocontrol of nematode and arthropod pests, such as insects and mites, with wide application in agriculture as an environmental friendly alternative to the use of chemical pesticides, therefore contributing to sustainable agriculture by reducing reliance on said chemical pesticides. The present invention teaches that, surprisingly, a genetic modification of a transcription factor-encoding gene, preferably Bbsmr1 or a functional homologue thereof, allowed increases virulence against pests. The invention also provides a method of controlling a pest, a seed treated with said genetically engineered fungi as well as a plant germinated therefrom.

The invention provides a double-root-system kiwi fruit grafted seedling cultivation method which comprises the following steps: S1, stock acquisition: respectively selecting one annual actinidia chinensis seedling and one delicious kiwi fruit seedling as stocks, pruning main roots of the two stocks to be 10-15cm in length, and reserving 4-5cm stems; a branch of a delicious kiwi fruit variety is selected as a scion, the length of the scion is 8-12 cm, the upper end of the scion is sealed with wax or a wound healing agent, and a notch in the lower end of the scion is cut into a smooth plane; s3, grafting: reversely grafting the two stocks at the lower end of the same scion by adopting a bark grafting or cleft grafting mode, and winding and wrapping a grafting part by using a grafting film to form a kiwi fruit seedling grafting body. Grafted seedlings cultivated by the method have two sets of root systems of actinidia valvata and actinidia deliciosa, so that the actinidia valvata and actinidia deliciosa grafted seedlings are high in waterlogging resistance, drought resistance and disease and pest resistance and can absorb comprehensive nutrients, and produced fruits are good in quality and high in yield and have high economic benefits.