151 results about "Secretory protein" patented technology

The invention discloses a decomposition preparation containing bacillus licheniformis as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The decomposition preparation provided by the invention comprises bacillus licheniformis and Thermus arcuatus. The bacillus licheniformis is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 27, 2025, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35039. The bacillus licheniformis is separated from coastal saline-alkali soil, has high temperature resistance and salt resistance, and can secrete protease and lipase at the same time. After the bacillus licheniformis and the Thermus arcuatus are mixed, the synergistic effect is remarkable, the duck-derived red mud is effectively promoted to be converted into humic acid, and high-value resource utilization of the duck-derived red mud is achieved.

The invention discloses a pharmaceutical composition for promoting angiogenesis and tissue regeneration and application thereof. The pharmaceutical composition of the present invention comprises an acidic secretory protein rich in cysteine, a disaccharide chain proteoglycan, and an XV-type collagen alpha1 chain. The biological material system loaded with the three types of proteins can promote angiogenesis, and can improve the tissue regeneration efficiency of bone, cartilage and skin defect areas.

The invention relates to the field of microorganisms, and discloses a P.megaterium and an application thereof in pig farm manure treatment, the P.megaterium is preserved in China Center for Type Culture Collection on December 13, 2022, the preservation address is Wuhan, China, the classification name of the P.megaterium is Pristia megaterium 3144, and the preservation number is CCTCC NO: M20221962. The content of pollutants such as total nitrogen, chemical oxygen demand and the like in pig farm feces is reduced by secreting various degrading enzymes such as protease, lipase and the like through the provided Priestia megaterium 3144 strain, and meanwhile, fecal coliforms and roundworm eggs are killed by the strain through metabolism heat production and antibacterial substance secretion.

The invention discloses an anti-lipopolysaccharide factor rALF-like protein and application thereof, and belongs to the technical field of molecular biology. The anti-lipopolysaccharide factor rALF-like protein provided by the invention belongs to secretory protein, the rALF-like protein can be competitively combined with an LPS structural domain of bacteria to achieve the purpose of inhibiting inflammation, and the expression of the coding gene Pv-ALF-like gene in blood cells, hepatopancreas, gill and intestinal tissues is remarkably up-regulated, so that the anti-lipopolysaccharide factor rALF-like protein can be used for inhibiting inflammation. The rALF-like protein disclosed by the invention has antibacterial and antiviral effects, lays a foundation for developing antibacterial and antiviral drugs and functional feed additives, and plays an important role in improving the resistance link of prawns.

The invention provides a combined lactobacillus salivarius YS-UR90 and a preparation method of the combined lactobacillus salivarius YS-UR90. The saliva combined lactobacillus provided by the invention can secrete protease and glycosidase, and the HCT and FLS gene expression levels of the saliva combined lactobacillus are high, so that the saliva combined lactobacillus can promote phenolic acid active ingredients to generate biotransformation during fermentation, and the proportion of chlorogenic acid and the like in fermentation liquor is increased. For example, marine fish oligopeptide and casein phosphopeptides are used as a composite nitrogen source, dandelion powder is used as a functional substrate, and a composite fermentation product obtained through fermentation of saliva and lactobacillus has the effect of promoting uric acid excretion and has a certain protection effect on kidney injury. The compound fermentation product has the effect of improving hyperuricemia mainly by inhibiting XOD activity, increasing serum urea nitrogen level, improving defect expression of uric acid synthesis gene HPRT1, inhibiting expression of uric acid reabsorption URAT1 and GLUT9 genes and improving expression of excretion genes OAT1 and ABCG2.

The invention provides application of Ustilaginoidea virens secretory protein UvWMT and a coding gene thereof in prevention and control of plant diseases. The gene sequence of the Ustilaginoidea virens secretory protein UvWMT is researched, found and cloned for the first time, and the new application of the Ustilaginoidea virens secretory protein UvWMT in disease-resistant breeding of crops such as rice is developed. Research finds that the protein is a key pathogenic factor in the process that rice is infected by ustilaginoidea virens, heterologous expression experiments further find that heterologous expression of UvWMT in rice can significantly improve the resistance of crops to various pathogenic bacteria such as ustilaginoidea virens, magnaporthe oryzae and xanthomonas oryzae, and has no significant difference in key agronomic traits compared with a wild type. An important theoretical basis is provided for rice disease-resistant breeding, crops can be safely and effectively endowed with broad-spectrum disease resistance by heterologous expression of the gene, and the gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

The invention relates to a preparation method of Parasin I protein or Parasin I fermentation liquor. The preparation method comprises the following steps: obtaining a yeast strain capable of secreting the Parasin I protein; inoculating the saccharomycetes strain into a specific liquid culture medium; fermenting the yeast strain and obtaining Parasin I fermentation liquor; the fermentation liquor comprises Parasin I protein; wherein the pH value of the specific culture medium is 6-9, the inoculum size of the saccharomycetes strain in the specific culture medium is 0.005-0.015 OD / mL, and the temperature of the fermentation saccharomycetes is 28-33 DEG C. The antibacterial peptide Parasin I protein or Parasin I fermentation liquor is simple in preparation method and high in yield, the immunity of fishes can be remarkably improved, and the survival rate of the fishes infected by pathogens is increased.

The application discloses a humification preparation containing bacillus licheniformis and a preparation method and application thereof, and belongs to the technical field of microorganisms. The humification preparation comprises the bacillus licheniformis and thermus arciformis. The bacillus licheniformis is preserved in the China General Microbiological Culture Collection Center on June 27, 2025, and the address of the preservation center is No. 3, Xili Beichen, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35039. The bacillus licheniformis is separated from a coastal saline-alkali soil, has high-temperature resistance and salt tolerance, and can secrete protease and lipase. After the bacillus licheniformis and the thermus arciformis are mixed, the synergistic effect is obvious, duck-derived red mud can be effectively converted into humic acid, and resourceful high-value utilization of the duck-derived red mud is realized.

The invention belongs to the technical field of genetic engineering and agricultural biology, and particularly relates to a plant pest prevention and control method based on an RNA interference technology. The invention provides application of dsRNA for specifically inhibiting expression of an RpSP1834 gene in preparation of a pesticide. The nucleotide sequence of the dsRNA is as shown in SEQ ID NO.7. The dsRNA has a nucleotide sequence as shown in SEQ ID NO.3. The dsRNA has a nucleotide sequence as shown in SEQ ID NO.4. The invention further provides a prevention and control method for the Eriomycorus cerinus. According to the invention, the pathogenicity of the apis cerana on the soybeans is reduced.

Methods of identifying and assays designed to identify at least one biomolecule from a patient are disclosed that comprise: collecting at least one biofluid from a patient, isolating at least one exosome from the biofluid, and identifying at least one biomolecule from the at least one exosome, wherein the at least one biomolecule is bound to the at least one exosome and is locally released from the patient. In some embodiments, the at least one biomolecule comprises a secretory protein, a neurotrophic factor, a growth factor, a cytokine, a chemokine, a pre-toxic molecule, a toxic molecule, or a combination thereof.

The invention discloses an IV-type secretory effect protein recognition method and system based on multi-modal information, and belongs to the technical field of bioinformatics and secretory protein recognition. The method comprises the following steps: firstly, extracting amino acid residue characteristics of protein by using a protein language model, and constructing a spatial adjacency graph of the protein by using a three-dimensional structure predicted by a protein structure prediction model; respectively extracting sequence features and structural features of the protein through a deep sequence module and a hierarchical graph module; meanwhile, a contrast learning module is introduced to realize cross-modal alignment of the same protein in a potential space; and performing bidirectional interaction on the sequence features and the structural features by using a cross attention module to obtain joint features, and finally outputting a classification result after nonlinear transformation by a gating linear unit for predicting the IV-type secretion effect protein. According to the invention, efficient coordination of sequence and structure bimodal information is realized, and the identification accuracy of the IV-type secretory effect protein is remarkably improved.

The application discloses a high-salt-tolerant protease-producing Arthrobacter protophormiae and application thereof, and belongs to the technical field of environmental microorganisms.The application provides the Arthrobacter protophormiae A1 which is preserved in the China Center for Type Culture Collection on May 23, 2024, and the preservation number is CCTCC NO: M 20241041.The Arthrobacter protophormiae provided by the application can tolerate 8.0% salinity, and can effectively secrete protease under high-salt stress.The bacteria can provide effective strain resources for the anaerobic biological treatment of reinforced kitchen waste and other high-salt high-protein waste water, thereby significantly reducing the treatment cost, and have important application value.

The application discloses a kind of marker for predicting oocyte copy number variation and application thereof, the marker includes the combination of CLEC11A, P4HB, EFEMP2, IL32, FTL, FLNA, COL6A3, ACPP and APOO protein.By the marker of the application, the expression level of granulosa cell secretory protein can be determined by granulosa cell transcriptome sequencing, and the genomic CNV condition of corresponding oocyte can be inferred.The detection object of the technical solution is discarded granulosa cell in assisted reproductive technology, so it will not have any impact on oocyte, and it is non-invasive;The present study is a transcriptome and methylation group detection from single cell level, with high-throughput characteristics, and all granulosa cells and oocytes are one-to-one matched, which can reflect the difference between single cell level COCs.

The invention specifically discloses application of VGF as a diagnostic biomarker for prostatic cancer, and relates to the technical field of biological medicines. The secretory protein VGF is identified to be remarkably up-regulated in prostatic fluid of a prostatic cancer patient before puncture for the first time, high expression of the secretory protein VGF is proved to be independently related to shorter progression-free lifetime and total lifetime through a public database, and a brand-new liquid biopsy marker is provided for PCa non-invasive early screening and prognosis evaluation.

The application provides a WNT5B-promoting tooth differentiation biological product and an application thereof. Compared with the prior art, the application has the following advantages: the patent integrates human embryonic tooth germ spatial transcriptome sequencing and epithelial cell and interstitial cell extracellular protein group sequencing data, screens signal molecules WNT5B and CTNNB1 secreted in the development process of tooth germ epithelium, and confirms the important role of WNT5B protein in tooth germ development for the first time. Compared with other WNT family members, WNT5B has stronger tooth-forming ability of promoting tooth-derived stem cells; the effective concentration of WNT5B is low, and 10 ng / ml is the optimal concentration of WNT5B for promoting tooth-derived stem cells to form teeth; and the role of WNT5B and CTNNB1 in extracellular secretion of tooth germ epithelial cells and the synergistic effect of WNT5B and CTNNB1 in promoting tooth differentiation of tooth germ mesenchymal cells are further determined, that is, the combined low-concentration use effect of the two is better than the single high-concentration use.

An engineered cell and its application are provided. The engineered cell has nucleic acid encoded by a secreted protein specifically integrated at a B2M locus. This provides MSCs cells that can achieve long-term stable expression of secreted proteins, thereby ensuring the sustained therapeutic effect of MSCs cells and reducing the cost of medication for patients.

Disclosed are methods and compositions for generating B lineage cells, such as plasmablasts, plasma cells, and protein-producing or protein-secreting B cells. The methods may relate to stage-specific differentiation from stem cells or stem-cell derived hematopoietic progenitors through one or more intermediates to B lineage cells, such as plasmablasts, plasma cells, and protein-producing or protein-secreting B cells.

The invention discloses a nanometer antibody of mycobacterium tuberculosis early secretory protein ESAT-6 and application thereof, relates to the technical field of antibody recombination and genetic engineering antibodies, and is characterized by providing two nanometer antibodies AEN1 and AEN5 which are derived from a total synthesis alpaca nanometer antibody yeast display library and are combined with ESAT-6 in high affinity, DNA sequences for coding the AEN1 and the AEN5, a carrier containing the DNA, a carrier containing the carrier containing the DNA, and a carrier containing the carrier containing the carrier containing the DNA. The invention relates to a vector, a host cell containing the vector, and a method for preparing AEN1 and AEN5 by utilizing genetic engineering. And the method for quantitatively detecting the ESAT-6 by using the double-antibody sandwich ELISA is established. Detection on clinical specimens of tuberculosis shows that the sensitivity of the diagnosis method is 94.9%, and the specificity is 100%. The antibody is used for preparing gold-labeled rapid test paper, and the ESAT-6 can be rapidly detected from serum of a mycobacterium tuberculosis infected patient.

The invention provides a secretory protein with insecticidal activity and an application of the secretory protein in pest control, an entomopathogenic fungus beauveria bassiana is used as a research material, and the secretory protein BbTsp1 is identified by using a protein spectrum technology. The protein is prepared and purified by using a prokaryotic expression system. A host blood cavity injection experiment proves that the protein has the activity of killing a host. Through a gene recombination technology, the BbTsp1 gene is introduced into beauveria bassiana, and an overexpression strain of the gene is constructed. Bioassay shows that the insecticidal activity of the strain is remarkably improved. The construction of a recombinant strain of the toxicity related gene identified by the invention improves the pest control efficiency of insecticidal fungi.

Disclosed are Schistosoma japonicum eggs and the ingredients of secretions and excretions thereof, comprising eggs, egg culture supernatant, and egg secreted proteins. (hereinafter referred to as “Schistosoma eggs and secretions and excretions thereof” for short). A Schistosoma infection can significantly inhibit metastatic tumors of organs such as lungs, and an anti-tumor effect is mediated by the “Schistosoma eggs and secretions and excretions thereof”, which have the effect of inhibiting various host tumors, comprising lung cancer, liver cancer, melanoma, and hematological tumors. Two egg secreted proteins having an anti-tumor effect are further identified. The “Schistosoma eggs and secretions and excretions thereof” exert an anti-tumor effect by activating natural immunity such as alveolar macrophages. The “Schistosoma eggs and secretions and excretions thereof” have a potential application value in prevention and treatment of tumors in humans.

The invention belongs to the field of biomedicine, and particularly relates to a construction method of an in-situ biological 3D printing artificial liver patch for treating hepatic failure. The main component of the artificial liver patch disclosed by the invention is composite hydrogel, and the composite hydrogel comprises hyaluronic acid (HA), gelatin (Gelatin), sodium alginate (sodium alginate) and methacrylated gelatin (GelMA). The composite hydrogel has good 3D printing performance, large-scale construction of the artificial liver patch can be achieved through biological 3D printing, and a potential solution is provided for transplantable biological artificial liver tissue. The artificial liver patch adopts an extrusion type printing mode, the forming speed is high, internal liver cells can still keep high activity, reconstruction of liver basic functions (glycogen storage, drug metabolism, secretory protein synthesis and the like) can be guaranteed, and the artificial liver patch has important potential in treatment of liver failure diseases.

The invention discloses a feline leukemia virus p27 protein monoclonal antibody, a hybridoma cell strain and application thereof, and relates to the technical field of biology, the amino acid sequence of a heavy chain variable region of the monoclonal antibody is as shown in SEQ ID NO.1 in a sequence table, and the amino acid sequence of a light chain variable region of the monoclonal antibody is as shown in SEQ ID NO.2 in the sequence table. According to the invention, a cell fusion technology is utilized to establish a hybridoma cell strain p27-3A5 capable of secreting a p27 protein monoclonal antibody, and a specific monoclonal antibody 3A5 is obtained. And the minimum linear epitope identified by the antibody is identified, so that the establishment of subsequent preparation of a diagnostic kit such as a colloidal gold test strip is facilitated.

This invention discloses a method and application for efficient synthesis of human lactoferrin through multi-copy integration, relating to the fields of genetic engineering and bioengineering. The invention provides a method for synthesizing human lactoferrin, including the steps of fermenting and culturing a recombinant Aspergillus niger strain, followed by isolation and purification to obtain human lactoferrin; the construction method of the recombinant Aspergillus niger strain includes the step of integrating the hLF gene into a gene locus in the host strain genome to construct the recombinant Aspergillus niger strain. This invention constructs a recombinant Aspergillus niger strain capable of recombinantly expressing human lactoferrin, initiating protein expression through a strongly inducible promoter, fusing endogenous high-secretion proteins, and optimizing the multi-copy integration method to integrate the lactoferrin expression cassette, achieving high-level synthesis of human lactoferrin, laying the foundation for the industrial production of lactoferrin or high-density fermentation of other food functional proteins using Aspergillus niger.

The invention discloses a method for enriching and extracting soluble protein in a tissue sample, which comprises the following steps: adding the tissue sample into a low-salt buffer solution, homogenizing, centrifuging, collecting supernatant precipitate, resuspending the precipitate by using Triton, and obtaining the supernatant to obtain an extracting solution; adding anhydrous acetonitrile to precipitate protein and collecting precipitate; carrying out proteolysis reductive alkylation and quenching reaction treatment on the precipitate to obtain a peptide fragment sample; desalting by using a peptide fragment sample desalting cleaning solution; after washing, adding a desalted eluent for eluting, collecting an eluent containing the peptide fragment, and drying to obtain a desalted peptide fragment; performing reversed-phase fractionation chromatography under an alkaline condition; and finally, LC-MS / MS detection is carried out. Under the condition that a strong denaturing agent is not introduced, secretory protein, cytoplasm protein and membrane protein can be selectively enriched, meanwhile, most of cell nucleus protein and tissue interstitial fibrin are effectively removed, and therefore the detection capacity of follow-up mass spectrometric detection on extremely-low-abundance circulating markers is remarkably improved.

The invention discloses an exocytosis protein subcellular localization method for mannitol-induced plasm-wall separation, and relates to the technical field of cytobiology, the method comprises the following specific steps: constructing a fusion expression vector: connecting a plant target exocytosis protein gene with a fluorescent protein gene through a gene cloning technology to obtain a fusion expression vector; inserting into a plant expression vector to obtain a recombinant expression vector containing the target extracellular secretory protein-fluorescent protein fusion gene; performing bacterial colony PCR identification and sequencing verification on the recombinant expression vector; by adopting an agrobacterium tumefaciens-mediated plant leaf instantaneous transformation technology, the fusion gene is rapidly expressed in plant cells, subsequent experiments can be carried out after culture is carried out for 2-3 days, the experiment period is greatly shortened, meanwhile, leaf cells are induced to be subjected to plasmon-wall separation through a gradient mannitol solution, positive cell screening and identification are not needed, and the application prospect is wide. And positioning can be realized by directly observing fluorescence signal distribution by using a laser confocal microscope.

The present invention relates to improved microbial enzymes with transfructosylation activity for efficient and cost-effective production of fructo-oligosaccharides. More specifically, the invention is directed towards obtaining mutant FTase family of genes from genus Aspergillus. Nucleic acids, peptide sequences, mutant proteins, vectors and host cells for recombinant expression of novel FTases are also provided. Various mutations, such as but not limited to point mutations and deletion mutations as well as combinations thereof are presented herein. The invention also relates to a process for the expression of a novel recombinant FTase mutants as a secreted protein. The enzymes exhibit high purity after filtration, which eliminates the need for costly chromatographic procedures.

The invention belongs to the field of gene engineering and the technical field of molecular biology, and particularly relates to double promoters for expressing sucrose phosphorylase and application of the double promoters. The double promoters comprise any one of the following sequences: a nucleotide sequence as shown in SEQ ID NO.1 or a complementary sequence of the nucleotide sequence; 1, and a nucleotide sequence as shown in SEQ ID NO. 2 or a complementary sequence thereof. The sucrose phosphorylase is synthesized in bacillus subtilis by adopting a double-promoter expression system, the effect of the sucrose phosphorylase is obviously superior to the expression effect of a single promoter P43, the extracellular enzyme activity is improved by 403%, and efficient production is realized. Meanwhile, the DNA fragment with the promoter and RBS functions provided by the invention can realize secretory expression of protein genes under the condition of not adding an inducer, provides an effective means for secretory expression of protein by bacillus subtilis by applying the DNA fragment to expression of sucrose phosphorylase, and has a very good development prospect.

The present invention provides the application of the secretory protein CTSB (cathepsin B) in predicting the risk of vascular lesions in patients with diabetic foot ulcers (DFUs), belonging to the field of biomedicine technology. Through single-cell sequencing studies, the present invention found that the secretory protein CTSB is significantly enriched in vascular endothelial cells in the blood vessels of DFU patients, with high PPI connectivity and low expression; CTSB overexpression / knockout experiments found that CTSB is associated with the proliferation and tubulogenesis of vascular endothelial cells in human blood vessels; ELISA experiments also found that the level of secretory protein CTSB in serum can be used as a marker for predicting the risk of vascular lesions in DFU patients, and that the expression level of CTSB is negatively correlated with the Wanger grade of diabetic foot; the method can predict the risk of peripheral vascular lesions in DFU patients, has the advantages of simple operation and high sensitivity, can reduce false detection and missed detection, and has stable and reliable test results, making it suitable for large-scale detection.

The invention relates to a bacillus licheniformis host cell for increasing production of secretory protein. In particular, the present invention relates to a Bacillus licheniformis host having a genetic modification that results in an increased production of heterologous, non-natural, secretory enzymes. Specifically, the present invention relates to a Bacillus licheniformis host cell which expresses a) a first polypeptide having peptidyl-prolyl cis-trans isomerase (EC 5.2. 1.8) activity comprising the amino acid sequence as shown in SEQ ID NO: 1, or at least 81% identical to SEQ ID NO: 1, and b) a second polypeptide having amylase activity comprising the amino acid sequence as shown in SEQ ID NO: 1, or at least 81% identical to SEQ ID NO: 2. Wherein the second polypeptide is heterologous to the Bacillus licheniformis host cell.