212 results about "Secretory protein" patented technology

The invention discloses a decomposition preparation containing bacillus licheniformis as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The decomposition preparation provided by the invention comprises bacillus licheniformis and Thermus arcuatus. The bacillus licheniformis is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 27, 2025, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35039. The bacillus licheniformis is separated from coastal saline-alkali soil, has high temperature resistance and salt resistance, and can secrete protease and lipase at the same time. After the bacillus licheniformis and the Thermus arcuatus are mixed, the synergistic effect is remarkable, the duck-derived red mud is effectively promoted to be converted into humic acid, and high-value resource utilization of the duck-derived red mud is achieved.

The invention discloses a pharmaceutical composition for promoting angiogenesis and tissue regeneration and application thereof. The pharmaceutical composition of the present invention comprises an acidic secretory protein rich in cysteine, a disaccharide chain proteoglycan, and an XV-type collagen alpha1 chain. The biological material system loaded with the three types of proteins can promote angiogenesis, and can improve the tissue regeneration efficiency of bone, cartilage and skin defect areas.

The invention provides a method for screening high-activity endogenous antibacterial peptides, which comprises the following steps: (1) collecting antibacterial peptide sequences in an antibacterial peptide database, and constructing an antibacterial peptide prediction model; (2) acquiring pig source proteome data; (3) screening out sequences with the length of 8-50 residues from pig-source proteome data to construct a candidate data set; (4) screening out non-redundant sequences from the candidate data set to construct a prediction data set; (5) predicting endogenous antibacterial peptides in the prediction data set by using the antibacterial peptide prediction model to obtain candidate antibacterial peptides; and (6) carrying out bioinformatics analysis on the candidate antibacterial peptide, comparing the candidate antibacterial peptide with an antibacterial peptide database, and screening out the antibacterial peptide with the similarity of less than 100% and secretory protein characteristics or exosome protein characteristics to obtain the high-activity endogenous antibacterial peptide. The method provided by the invention can be used for efficiently and quickly screening the high-activity endogenous antibacterial peptide, provides a new way for developing novel antibacterial peptide, and has a wide application prospect.

The invention provides a hybridoma cell strain secreting a grass carp reovirus VP4 protein monoclonal antibody as well as the monoclonal antibody and application thereof, and belongs to the technical field of aquatic reovirus detection. The hybridoma cell strain is named as GCRV-VP4-6E7B12, and is preserved in the China Center for Type Culture Collection on December 10, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: C2024160. The grass carp reovirus VP4 protein monoclonal antibody 6E7B12 secreted by the hybridoma cell strain GCRV-VP4-6E7B12 has the advantage of being specifically combined with an antigen. The enzyme-labeled monoclonal antibody is used as a detection antibody, the VP4 protein polyclonal antibody is used as a capture antibody, the content of the grass carp reovirus VP4 protein in a sample to be detected can be quantitatively detected by adopting a double-antibody sandwich ELISA method, the detection range is 1-32 ng / mL, and the lower detection limit is 1 ng / mL. The detection method has the advantages of high sensitivity and wide detection range.

The invention relates to the field of microorganisms, and discloses a P.megaterium and an application thereof in pig farm manure treatment, the P.megaterium is preserved in China Center for Type Culture Collection on December 13, 2022, the preservation address is Wuhan, China, the classification name of the P.megaterium is Pristia megaterium 3144, and the preservation number is CCTCC NO: M20221962. The content of pollutants such as total nitrogen, chemical oxygen demand and the like in pig farm feces is reduced by secreting various degrading enzymes such as protease, lipase and the like through the provided Priestia megaterium 3144 strain, and meanwhile, fecal coliforms and roundworm eggs are killed by the strain through metabolism heat production and antibacterial substance secretion.

The invention discloses an anti-lipopolysaccharide factor rALF-like protein and application thereof, and belongs to the technical field of molecular biology. The anti-lipopolysaccharide factor rALF-like protein provided by the invention belongs to secretory protein, the rALF-like protein can be competitively combined with an LPS structural domain of bacteria to achieve the purpose of inhibiting inflammation, and the expression of the coding gene Pv-ALF-like gene in blood cells, hepatopancreas, gill and intestinal tissues is remarkably up-regulated, so that the anti-lipopolysaccharide factor rALF-like protein can be used for inhibiting inflammation. The rALF-like protein disclosed by the invention has antibacterial and antiviral effects, lays a foundation for developing antibacterial and antiviral drugs and functional feed additives, and plays an important role in improving the resistance link of prawns.

The invention discloses bacillus Witzmannii for inhibiting phytopathogen and application of the bacillus Witzmannii, relates to the field of biological prevention and control, and aims to solve the problem that chemical prevention and control methods of phytopathogen cause negative effects on ecological environment and human health. The bacillus wiedmannii is bacillus wiedmannii SJZ and is preserved in the China Center for Type Culture Collection, the preservation date is December 23, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20242884. The bacillus vetzmannii disclosed by the invention can be used for inhibiting sclerotinia sclerotiorum and solanum nigrum. And protease, cellulase and chitinase can be secreted. The bacillus vetzmannii is used for nitrogen fixation and can secrete siderophores and IAA (indoleacetic acid).

The invention provides a combined lactobacillus salivarius YS-UR90 and a preparation method of the combined lactobacillus salivarius YS-UR90. The saliva combined lactobacillus provided by the invention can secrete protease and glycosidase, and the HCT and FLS gene expression levels of the saliva combined lactobacillus are high, so that the saliva combined lactobacillus can promote phenolic acid active ingredients to generate biotransformation during fermentation, and the proportion of chlorogenic acid and the like in fermentation liquor is increased. For example, marine fish oligopeptide and casein phosphopeptides are used as a composite nitrogen source, dandelion powder is used as a functional substrate, and a composite fermentation product obtained through fermentation of saliva and lactobacillus has the effect of promoting uric acid excretion and has a certain protection effect on kidney injury. The compound fermentation product has the effect of improving hyperuricemia mainly by inhibiting XOD activity, increasing serum urea nitrogen level, improving defect expression of uric acid synthesis gene HPRT1, inhibiting expression of uric acid reabsorption URAT1 and GLUT9 genes and improving expression of excretion genes OAT1 and ABCG2.

The present disclosure relates to methods for increasing recovery and purity of a secreted protein of interest, recovered proteins from the methods, and uses of the proteins.

The invention provides application of Ustilaginoidea virens secretory protein UvWMT and a coding gene thereof in prevention and control of plant diseases. The gene sequence of the Ustilaginoidea virens secretory protein UvWMT is researched, found and cloned for the first time, and the new application of the Ustilaginoidea virens secretory protein UvWMT in disease-resistant breeding of crops such as rice is developed. Research finds that the protein is a key pathogenic factor in the process that rice is infected by ustilaginoidea virens, heterologous expression experiments further find that heterologous expression of UvWMT in rice can significantly improve the resistance of crops to various pathogenic bacteria such as ustilaginoidea virens, magnaporthe oryzae and xanthomonas oryzae, and has no significant difference in key agronomic traits compared with a wild type. An important theoretical basis is provided for rice disease-resistant breeding, crops can be safely and effectively endowed with broad-spectrum disease resistance by heterologous expression of the gene, and the gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

The invention relates to a preparation method of Parasin I protein or Parasin I fermentation liquor. The preparation method comprises the following steps: obtaining a yeast strain capable of secreting the Parasin I protein; inoculating the saccharomycetes strain into a specific liquid culture medium; fermenting the yeast strain and obtaining Parasin I fermentation liquor; the fermentation liquor comprises Parasin I protein; wherein the pH value of the specific culture medium is 6-9, the inoculum size of the saccharomycetes strain in the specific culture medium is 0.005-0.015 OD / mL, and the temperature of the fermentation saccharomycetes is 28-33 DEG C. The antibacterial peptide Parasin I protein or Parasin I fermentation liquor is simple in preparation method and high in yield, the immunity of fishes can be remarkably improved, and the survival rate of the fishes infected by pathogens is increased.

The application discloses a humification preparation containing bacillus licheniformis and a preparation method and application thereof, and belongs to the technical field of microorganisms. The humification preparation comprises the bacillus licheniformis and thermus arciformis. The bacillus licheniformis is preserved in the China General Microbiological Culture Collection Center on June 27, 2025, and the address of the preservation center is No. 3, Xili Beichen, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35039. The bacillus licheniformis is separated from a coastal saline-alkali soil, has high-temperature resistance and salt tolerance, and can secrete protease and lipase. After the bacillus licheniformis and the thermus arciformis are mixed, the synergistic effect is obvious, duck-derived red mud can be effectively converted into humic acid, and resourceful high-value utilization of the duck-derived red mud is realized.

The invention belongs to the technical field of genetic engineering and agricultural biology, and particularly relates to a plant pest prevention and control method based on an RNA interference technology. The invention provides application of dsRNA for specifically inhibiting expression of an RpSP1834 gene in preparation of a pesticide. The nucleotide sequence of the dsRNA is as shown in SEQ ID NO.7. The dsRNA has a nucleotide sequence as shown in SEQ ID NO.3. The dsRNA has a nucleotide sequence as shown in SEQ ID NO.4. The invention further provides a prevention and control method for the Eriomycorus cerinus. According to the invention, the pathogenicity of the apis cerana on the soybeans is reduced.

Methods of identifying and assays designed to identify at least one biomolecule from a patient are disclosed that comprise: collecting at least one biofluid from a patient, isolating at least one exosome from the biofluid, and identifying at least one biomolecule from the at least one exosome, wherein the at least one biomolecule is bound to the at least one exosome and is locally released from the patient. In some embodiments, the at least one biomolecule comprises a secretory protein, a neurotrophic factor, a growth factor, a cytokine, a chemokine, a pre-toxic molecule, a toxic molecule, or a combination thereof.

The invention discloses a method for increasing the passage frequency of a mammary epithelial cell line and application of the method, and belongs to the technical field of mammary epithelial cell lines. It is found for the first time that an insulator-stent attachment area exogenous fragment HS4-SAR fragment is connected with a p-Ef1a-SV40LT-Puro vector or a pEF1a-eGFP vector, commercial immortalized cell lines and primary cell lines are transfected, the passage number of mammary gland epithelial cell lines can be remarkably increased, and the mammary gland epithelial cell lines can be obtained. After 120 generations of passage, the cell morphology is still normal, malignant transformation is avoided, and the gene expression level is still not reduced, so that immortalization is realized; the established milk goat mammary gland epithelial cell line still has the capability of synthesizing and secreting protein; the provided method for increasing the passage frequency of the mammary epithelial cell line is simple and easy to operate, has a remarkable effect on an immortalized cell line and a primary cell line, and is expected to increase the passage frequency of stable passage of various mammary epithelial cell lines and reduce the cell cost of related research.

The invention discloses high-salt-tolerance arthrobacter vitriomyzae capable of producing protease and application of the arthrobacter vitriomyzae, and belongs to the technical field of environmental microorganisms. The invention provides an arthrobacter pseudomonas A1 which is preserved in the China Center for Type Culture Collection on May 23, 2024, and the preservation number of the arthrobacter pseudomonas A1 is CCTCC (China Center for Type Culture Collection) NO: M 20241041. The arthrobacter sp. Can tolerate the salinity of 8.0%, and can still effectively secrete protease under the stress of high salt. The strain can provide an effective strain resource for strengthening anaerobic biological treatment of high-salt and high-protein wastewater such as kitchen wastewater, so that the treatment cost is remarkably reduced, and the strain has important application value.

The invention discloses an IV-type secretory effect protein recognition method and system based on multi-modal information, and belongs to the technical field of bioinformatics and secretory protein recognition. The method comprises the following steps: firstly, extracting amino acid residue characteristics of protein by using a protein language model, and constructing a spatial adjacency graph of the protein by using a three-dimensional structure predicted by a protein structure prediction model; respectively extracting sequence features and structural features of the protein through a deep sequence module and a hierarchical graph module; meanwhile, a contrast learning module is introduced to realize cross-modal alignment of the same protein in a potential space; and performing bidirectional interaction on the sequence features and the structural features by using a cross attention module to obtain joint features, and finally outputting a classification result after nonlinear transformation by a gating linear unit for predicting the IV-type secretion effect protein. According to the invention, efficient coordination of sequence and structure bimodal information is realized, and the identification accuracy of the IV-type secretory effect protein is remarkably improved.

The application discloses a high-salt-tolerant protease-producing Arthrobacter protophormiae and application thereof, and belongs to the technical field of environmental microorganisms.The application provides the Arthrobacter protophormiae A1 which is preserved in the China Center for Type Culture Collection on May 23, 2024, and the preservation number is CCTCC NO: M 20241041.The Arthrobacter protophormiae provided by the application can tolerate 8.0% salinity, and can effectively secrete protease under high-salt stress.The bacteria can provide effective strain resources for the anaerobic biological treatment of reinforced kitchen waste and other high-salt high-protein waste water, thereby significantly reducing the treatment cost, and have important application value.

The application discloses a kind of marker for predicting oocyte copy number variation and application thereof, the marker includes the combination of CLEC11A, P4HB, EFEMP2, IL32, FTL, FLNA, COL6A3, ACPP and APOO protein.By the marker of the application, the expression level of granulosa cell secretory protein can be determined by granulosa cell transcriptome sequencing, and the genomic CNV condition of corresponding oocyte can be inferred.The detection object of the technical solution is discarded granulosa cell in assisted reproductive technology, so it will not have any impact on oocyte, and it is non-invasive;The present study is a transcriptome and methylation group detection from single cell level, with high-throughput characteristics, and all granulosa cells and oocytes are one-to-one matched, which can reflect the difference between single cell level COCs.

The invention specifically discloses application of VGF as a diagnostic biomarker for prostatic cancer, and relates to the technical field of biological medicines. The secretory protein VGF is identified to be remarkably up-regulated in prostatic fluid of a prostatic cancer patient before puncture for the first time, high expression of the secretory protein VGF is proved to be independently related to shorter progression-free lifetime and total lifetime through a public database, and a brand-new liquid biopsy marker is provided for PCa non-invasive early screening and prognosis evaluation.

The application provides a WNT5B-promoting tooth differentiation biological product and an application thereof. Compared with the prior art, the application has the following advantages: the patent integrates human embryonic tooth germ spatial transcriptome sequencing and epithelial cell and interstitial cell extracellular protein group sequencing data, screens signal molecules WNT5B and CTNNB1 secreted in the development process of tooth germ epithelium, and confirms the important role of WNT5B protein in tooth germ development for the first time. Compared with other WNT family members, WNT5B has stronger tooth-forming ability of promoting tooth-derived stem cells; the effective concentration of WNT5B is low, and 10 ng / ml is the optimal concentration of WNT5B for promoting tooth-derived stem cells to form teeth; and the role of WNT5B and CTNNB1 in extracellular secretion of tooth germ epithelial cells and the synergistic effect of WNT5B and CTNNB1 in promoting tooth differentiation of tooth germ mesenchymal cells are further determined, that is, the combined low-concentration use effect of the two is better than the single high-concentration use.

An engineered cell and its application are provided. The engineered cell has nucleic acid encoded by a secreted protein specifically integrated at a B2M locus. This provides MSCs cells that can achieve long-term stable expression of secreted proteins, thereby ensuring the sustained therapeutic effect of MSCs cells and reducing the cost of medication for patients.

Disclosed are methods and compositions for generating B lineage cells, such as plasmablasts, plasma cells, and protein-producing or protein-secreting B cells. The methods may relate to stage-specific differentiation from stem cells or stem-cell derived hematopoietic progenitors through one or more intermediates to B lineage cells, such as plasmablasts, plasma cells, and protein-producing or protein-secreting B cells.

The invention discloses a wheat scab disease-resistant protein TaSnRK1alpha and fusarium graminearum secretory protein Osp24 interaction key site and application thereof, and relates to the technical field of bioengineering. According to the invention, TaSnRK1alpha protein structural domain distribution and sequence characteristics are analyzed, the TaSnRK1alpha protein is divided into multiple forms, and a yeast two-hybrid test is utilized to identify that the 479th valine of the TaSnRK1alpha protein is a key site for determining interaction with secretory protein Osp24. After the sites are mutated, the targeting of the fusarium graminearum secretory protein Osp24 is blocked. Therefore, an ideal candidate editing target spot is provided for accurately modifying TaSnRK1 alpha by utilizing a CRISPR-Cas9 technology, the targeting and inhibition of fusarium graminearum on TaSnRK1 alpha can be effectively avoided, the gibberellic disease resistance is improved, and a gibberellic disease resistant wheat material is created.

The invention discloses a nanometer antibody of mycobacterium tuberculosis early secretory protein ESAT-6 and application thereof, relates to the technical field of antibody recombination and genetic engineering antibodies, and is characterized by providing two nanometer antibodies AEN1 and AEN5 which are derived from a total synthesis alpaca nanometer antibody yeast display library and are combined with ESAT-6 in high affinity, DNA sequences for coding the AEN1 and the AEN5, a carrier containing the DNA, a carrier containing the carrier containing the DNA, and a carrier containing the carrier containing the carrier containing the DNA. The invention relates to a vector, a host cell containing the vector, and a method for preparing AEN1 and AEN5 by utilizing genetic engineering. And the method for quantitatively detecting the ESAT-6 by using the double-antibody sandwich ELISA is established. Detection on clinical specimens of tuberculosis shows that the sensitivity of the diagnosis method is 94.9%, and the specificity is 100%. The antibody is used for preparing gold-labeled rapid test paper, and the ESAT-6 can be rapidly detected from serum of a mycobacterium tuberculosis infected patient.

The invention provides a secretory protein with insecticidal activity and an application of the secretory protein in pest control, an entomopathogenic fungus beauveria bassiana is used as a research material, and the secretory protein BbTsp1 is identified by using a protein spectrum technology. The protein is prepared and purified by using a prokaryotic expression system. A host blood cavity injection experiment proves that the protein has the activity of killing a host. Through a gene recombination technology, the BbTsp1 gene is introduced into beauveria bassiana, and an overexpression strain of the gene is constructed. Bioassay shows that the insecticidal activity of the strain is remarkably improved. The construction of a recombinant strain of the toxicity related gene identified by the invention improves the pest control efficiency of insecticidal fungi.

The invention provides a WNT5B dental differentiation promoting biological product and application thereof. Compared with the prior art, the application has the following advantages: by integrating human embryo tooth embryo space transcriptome sequencing data and epithelial cell and interstitial cell exocrine proteome sequencing data, signal molecules WNT5B and CTNNB1 secreted in the tooth embryo epithelial development process are screened out, the important effect of the WNT5B protein in tooth embryo development is confirmed for the first time, and the application has a wide application prospect. Compared with other WNT family members, the WNT5B has higher capability of promoting the tooth formation of the odontogenic stem cells; the onset concentration of the WNT5B is low, and the onset concentration is the optimal concentration of the WNT5B for promoting the odontogenic stem cells to form teeth when the onset concentration is 10ng / ml; the effects of the WNT5B and the CTNNB1 in tooth embryo epithelial cell exocrine and the synergistic effect of the WNT5B and the CTNNB1 in promoting tooth embryo mesenchymal cell odontogenic differentiation are further determined, namely the combined low-concentration use effect of the WNT5B and the CTNNB1 is superior to that of single high-concentration use of the WNT5B and the CTNNB1.

Disclosed are Schistosoma japonicum eggs and the ingredients of secretions and excretions thereof, comprising eggs, egg culture supernatant, and egg secreted proteins. (hereinafter referred to as “Schistosoma eggs and secretions and excretions thereof” for short). A Schistosoma infection can significantly inhibit metastatic tumors of organs such as lungs, and an anti-tumor effect is mediated by the “Schistosoma eggs and secretions and excretions thereof”, which have the effect of inhibiting various host tumors, comprising lung cancer, liver cancer, melanoma, and hematological tumors. Two egg secreted proteins having an anti-tumor effect are further identified. The “Schistosoma eggs and secretions and excretions thereof” exert an anti-tumor effect by activating natural immunity such as alveolar macrophages. The “Schistosoma eggs and secretions and excretions thereof” have a potential application value in prevention and treatment of tumors in humans.

The invention belongs to the field of biomedicine, and particularly relates to a construction method of an in-situ biological 3D printing artificial liver patch for treating hepatic failure. The main component of the artificial liver patch disclosed by the invention is composite hydrogel, and the composite hydrogel comprises hyaluronic acid (HA), gelatin (Gelatin), sodium alginate (sodium alginate) and methacrylated gelatin (GelMA). The composite hydrogel has good 3D printing performance, large-scale construction of the artificial liver patch can be achieved through biological 3D printing, and a potential solution is provided for transplantable biological artificial liver tissue. The artificial liver patch adopts an extrusion type printing mode, the forming speed is high, internal liver cells can still keep high activity, reconstruction of liver basic functions (glycogen storage, drug metabolism, secretory protein synthesis and the like) can be guaranteed, and the artificial liver patch has important potential in treatment of liver failure diseases.