171 results about "Apis cerana" patented technology

A multi-layer overlaying circular frameless apis cerana cerana beehive capable of greatly reducing labor capacity and producing pure primitive ecological apis cerana cerana honey is an integral beehive formed by overlaying a plurality of circular following beehives on a circular base plate machined with a concave natural deslagging slope surface, a deslagging hole, a nest entrance and a feeding hole, placing four metal pads of the following beehives on each following beehive, covering the top following beehive with a circular beehive cover with a vent hole, and vertically penetrating and connecting the base plate and connection holes of a four-corner connection clamp on the box cover through four L-shaped reinforcing bars. Therefore, the apis cerana cerana group can naturally form a sphere apis cerana cerana bulk in the beehives, and nests from the top down; and if the stored honey at the uppermost layer is mature, sealed and covered, the four L-shaped reinforcing bars and the corresponding metal pads are detached, the following beehive at the uppermost layer is transversely cut to take out the honey, and then is additionally arranged on the base plate at the bottom layer; and the survival and propagation of the apis cerana cerana after the honey is taken out are not influenced, the output and quality of apis cerana cerana honey are improved, and the multi-layer overlaying circular frameless apis cerana cerana beehive has a vital significance for apis cerana cerana protection.

Artificial cultivation method of apis cerana queen, first preparing artifical stylobate of apis mellifera, and choosing a group of apis mellifera queen as feeding group for larva of apis cerana queen; putting the artifical stylobate of apis mellifera into a selected feeding group of apis mellifera group, taking the artifical stylobate out after cleaned by worker bee of the apis mellifera, transfering larva of worker bee in mother apis cerana group that is in need of cultivating queen to the artifical stylobate; at last putting the artifical stylobate into generation box of the selected apis mellifera feeding group for feeding larva of apis cerana queen, after 11 to 12 days the apis cerana queen stylobate is mature, taking out the apis cerana queen stylobate before the virgin queen going out the room, introducing the virgin queen to apis cerana mating group for following mating, so as to become a spawning queen. The superiority of the invention is: obtaining large amount of mature apis cerana queen stylobate or virgin queen for one time; because of adequent apis mellifera bee milk, bigger and stronger virgin queen can be cultivated; apis mellifera and apis cerana of different species can generates nutritious heterosis which provides important meaning for apis cerana species cultivation.

The invention discloses an Apis cerana royal jelly antibacterial peptide AccRoyalisin gene and an encoded polypeptide thereof and application thereof. The gene has a DNA sequence shown as SEQ ID No.4; and the encoded protein of the gene has an amino acid sequence shown as SEQ ID No.5. The antibacterial peptide gene can establish a high-expression genetic engineering strain by building an Escherichia coli expression vector, and performing producing and separating and purifying on a recombinant product through induction expression of the fermentation engineering. The recombinant product thereof has an obvious inhibiting effect on a plurality of gram positive bacteria, such as bacillus subtilis, staphylococcus aureus and micrococcus flavus and the like, can be used for preserving and refreshing foods, in particular for preventing food-born bacterial contamination, is harmless to human body and animals, can serve as a substitute of antibiotics and chemical preservatives and is also suitable for preventing and controlling bred animal bacterial diseases. The Apis cerana royal jelly antibacterial peptide AccRoyalisin gene and the encoded polypeptide thereof lay a foundation for the development, the massive industrial production and the application of the Royalisin.

The invention relates to a foundation-free section honey production method of Apis ceranas, which replaces section honey lattices by special section honey frames, replaces queen separation plates by separation plates and replaces purpose-made shallow boxes by standard boxes. The section honey production method of the Apis ceranas comprises the flowing steps of: manufacture of the special section honey frames and the separation plates, obtaining of non-husks natural spleen bases, fixation of spleen bases, fixation of the section honey frames, production of the section honey of the Apis ceranas, section honey cutting and section honey package. The production method is simple and practical, reduces the production cost and has low cost. The section honey has better quality and richer fragrance. The honey yield can be increased because no queen plate is adopted. The production method changes complexity into simplicity, reduces the labor intensity, has low technical requirements, is easy to learn and master by beekeepers and has low labor intensity in the production process. Strong colony is easy to maintain because the space of honey boxes is increased. The nest insect harm is reduced.

The invention provides a method for identifying the honey of Apis cerana and the honey of Apis mellifera. The method achieves the purpose of identifying the two kinds of honey based on the differenceof alkane components in the two kinds of honey. Honey samples are extracted with petroleum ether and then detected via gas chromatography-mass spectrometry; and detection results show that the alkanecomponents of the honey of Apis cerana and the honey of Apis mellifera are different, the honey of Apis cerana contains C37 alkanes, and the honey of Apis mellifera contains C31 alkanes. It is confirmed that the component difference is only related to bee species after detection and analysis of a large number of samples, and the influence of plant sources on identification is excluded. The differential alkane components are stable in honey and are not affected by concentration processing and shelf life. The method of the invention is reasonable in design, convenient, quick and accurate in identification, and is applicable to both the identification of the sources of raw honey and commercial bee species and the identification of the honey of Apis cerana blended with a certain proportion ofthe honey of Apis mellifera.

The invention provides a method for identifying apis cerana honey and apis mellifera honey, which achieves the purpose of identifying the apis cerana honey and the apis mellifera honey according to the specific band difference between the proteins of the apis cerana honey and the apis mellifera honey. After an apis cerana honey sample is treated, protein electrophoresis is conducted, the apis cerana honey has a 15.0KDa protein band, the apis mellifera honey has a 16.5KDa protein band, and a mixed honey sample of the both has both the 15.0KDa protein band and the 16.5KDa protein band. By differentiating the specific protein bands of the different honeys to achieve the purpose of identification, the method can accurately identify the apis cerana honey and the apis mellifera honey. In the method disclosed by the invention, the target proteins stably exist in the apis cerana honey and the apis mellifera honey, not affected by honey resources, concentration processing and shelf life. The design of the method disclosed by the invention is reasonable and simple, instruments are common, cost is low, identification is accurate, and the method is also suitable for identifying a certain proportion of apis mellifera honey mixed in the apis cerana honey.

The invention discloses an apis cerana queen breeding method and belongs to bee queen breeding methods. By means of the apis cerana queen breeding method, a bred queen can be large in size, strong, meek in temperament and high in egg production capacity. Bowl-shaped queen cells made of bee wax are fixed to a honeycomb frame, and a queen breeding frame is obtained; the queen breeding frame is put in a queen breeding apis cerana swarm, and worker bees are made to clean the queen cells; the inner walls of the queen cells are brushed with fresh bee milk, eggs of other apis cerana swarms are implanted into the queen cells, and the queen breeding frame is coated with apis mellifera honey in a dipping mode and then put back to the queen breeding apis cerana swarm; the queen breeding frame is taken out and put into an apis mellifera swarm for nursing for 12 hours, then the queen breeding frame is taken out and put back to the queen breeding apis cerana swarm again for breeding for 5-7 days, and a mature queen embryo is obtained; a virgin queen can be hatched on the 9th or 10th day. The queen bred through the method is large in size, strong, high in disease resistance and high in egg production capacity, the bee swarm is meek in temperament, and the bee robbing or honey stealing phenomenon can be avoided even bee swarms are away by 10 cm, and the apis cerana queen breeding method is used for breeding apis cerana.

The invention discloses a method for breeding special pollination apis cerana. The method includes steps of 1), selecting breeding materials: selecting a high-quality and high-production apis cerana species from a first-grade breeding apis cerana field of the Zhejiang province, introducing high-quality apis cerana from the Hainan province, and combining the high-quality and high-production apis cerana species, the high-quality apis cerana and local high-quality, high-production and gentle apis cerana difficult in swarming with one another to obtain the breeding materials; 2), performing three-way cross breeding: utilizing the apis cerana of the Hainan province as male parents and Fuchide apis cerana as female parents, performing cross breeding and directive breeding on the male parents and the female parents by an artificial insemination mode, selecting a high-quality, high-production, high-temperature-resistant, high-moisture-resistant and weak-swarming apis cerana species as breeding female parents for a next apis cerana generation, selecting local high-quality apis cerana as male parents and performing cross breeding on the local high-quality apis cerana and the cross breeding female parents; 3), locked breeding: creating 60 high-quality apis cerana colonies with three female parent characteristics, and enabling the high-quality apis cerana colonies to perform natural mating under an isolated natural condition. The method has the advantages that various technical bottlenecks in the aspect of bee pollination can be solved for greenhouse fruit and vegetable growers, and the bee pollination efficiency and the bee pollination quality are improved for greenhouses.

The invention discloses a method for improving the productivity of Apis cerana cerana by organizing strong colonies through a mode of putting three queen bees in the same beehive. The method for improving the productivity of the Apis cerana cerana by organizing the strong colonies through the mode of putting the three queen bees in the same beehive is characterized in that the three queen bees are put into the same beehive and isolated through queen excluders, but colonies are not isolated, and one of the queen bees is prevented from oviposition alternately and is in a state of rest; then the three queen bees have a rest alternately and regularly and achieve oviposition alternately to achieve the optimum state of oviposition and the highest honey yield. According to the method for improving the productivity of the Apis cerana cerana by organizing the strong colonies through the mode of putting the three queen bees in the same beehive, the aim that 17 combs of the strong colonies can obtain a high yield of 90-12kg per strong colony per year can be maintained through the simplest method and the lowest cost. The method for improving the productivity of the Apis cerana cerana by organizing the strong colonies through the mode of putting the three queen bees in the same beehive is very ideal in effect, needs little investment and becomes effective very quickly.

The invention relates to a firm and durable steel-wood structured Apis cerana Smith activity frame which can enable Apis cerana Smith to go up and down a super smoothly, wherein the steel-wood structured Apis cerana Smith activity frame is formed by combining an upper concave metal beam, a lower concave metal beam, two wood edge strips and a fine iron wire by using iron nails; and the upper beam and the lower beam are about 1 mm in thickness respectively. According to the invention, with the all-metal Apis cerana Smith bee-way queen excluder (patent number: 201210142959.x) disclosed by the invention, separation of brood rearing and honey producing of Apis cerana Smith is realized; quality and yield of Apis cerana Smith honey are increased; and the problem of raising Apis cerana Smith by using the super is solved.

The invention discloses an elite breeding method for apis cerana in the southern area of China. A swarm with good fertility performance and uniform shape feature can be kept as a population; by means of four-generation advantageous hybridization propagation, high-fertility of the apis cerana on Changbai mountains, disease-resistance of the apis cerana in Aba county, cold-resistance of the apis cerana in the northern area and heat-resistance of the apis cerana in Hainan province are taken into consideration, and accordingly the population, with good comprehensive characteristics, of the apis cerana in the southern area of China is bred; growth of the apis cerana in the southern area of China is stabilized, and the apis cerana in the southern area of China can still keep good characteristics after inheritance of multiple generations. Parents of good apis cerana are led in from different areas of China, by means of an advantageous hybridization propagation technique, the good population, with disease resistance, high-temperature and low-temperature resistance, high yield and high accumulated nutrition, of the apis cerana in the southern area of China is obtained after four-generation hybridization breeding, good characteristics of offsping of the apis cerana in southern area of China are maintained through concentrated scientific breeding and artificial breeding, and a southern apis cerana breeding technique is developed by summarizing bee breeding techniques and apis cerana breeding techniques in the southern area of China.

A wall-hung D-shaped frameless Apis cerana hive is characterized in that a D-shaped base ring is placed on a D-shaped metal bracket, a concave base plate is mounted below the D-shaped metal bracket, 4-6 D-shaped supers are stacked on the D-shaped base ring, a D-shaped cover covers the supers, and gaps between the D-shaped supers and gaps between the D-shaped supers and the cover are sealed by bee wax. The wall-hung D-shaped frameless Apis cerana hive has the advantages that Apis cerana is allowed to form spherical clusters, viewing, swarming and honey taking are convenient, and honey is prevented from secondary pollution; the Apis cerana hive which accords to natural living habits of Apis cerana is suitable for Apis cerana beekeeping in permanent apiary in scattered families in villages and modern villas, the Apis cerana hive combines advantages of novel Apis cerana hives and traditional Apis cerana hives and overcomes defects of the novel Apis cerana hives and the traditional Apis cerana hives, the Apis cerana hive is free of usual management and feeding, and the honey produced is purely natural, original, and various in kinds; the wall-hung D-shaped frameless Apis cerana hive is simple to mount, convenient to assemble, firm and durable, and long in service life.

The invention relates to a method for distinguishing distinguishing Apis cerana cerana honey from Apis mellifera ligustica honey. The method comprises the steps of (1) weighing a detected amount of honey sample to be detected; (2) detecting volatile hydrocarbon compounds in the honey to be detected by using HS-SPME-GC-MS to obtain a detection spectrogram, and quantifying Cis-9-Tricosene, (C9T), isomers thereof, namely, Iso-9-Tricosene, Cis-9-Pentacosene, (C9P) and an isomer Iso-9-Pentacosene in the honey sample to be detected according to the spectrogram; and (3) identifying the Apis cerana cerana honey and Apis mellifera ligustica honey according to the contents of C9T and C9P in the honey and the proportions of C9T and C9P to respective isomers.

The invention relates to a method for rapidly reproducing Apis cerana by using a movable frame capable of overturning a lower part of a honeycomb. Beewax connected to the honey comb, on the upper part of a circular frame ring is cut while the area of honey stored in the upper part of the circular frame ring, of the Apis cerana is more than 1/3 of the circular frame ring; the circular frame ring is overturned at an angle of 180 degrees, the honey stored in the circular fame ring faces downward, and eggs, larva and pupae in the circular frame ring face upward. The method meets a natural life habit of the Apis cerana, can expand the egg laying range of a queen bee, can rapidly reproduce the Apis cerana, is conductive to protection and development of the Apis cerana, and can breed Apis mellifera and other bee species.

The invention discloses a plant fragrance smell activator formula screening method based on apis cerana odorant binding protein. The method comprises the following process steps: 1) extracting working bee antenna total RNA (Ribonucleic Acid) of apis cerana; 2) designing an apis cerana odorant binding protein gene primer, and acquiring the full length of an apis cerana odorant binding protein gene through RT-PCR (Reverse Transcription-Polymerase Chain Reaction); 3) building a prokaryotic expression vector of the apis cerana odorant binding protein gene; 4) inducing recombinant expression of the apis cerana odorant binding protein through IPTG (isopropyl-beta-d-thiogalactoside), and performing purification through a Ni Sepharose FF affinity column; 5) acquiring a combined response spectrum of the apis cerana odorant binding protein and plant fragrance smell through a competitive fluorescent combination method, and determining the combined response spectrum as the plant fragrance smell activator suitable for the apis cerana when the dissociation constant KD is lower than 20mummol/L.

The invention discloses a method for preparing an Apis cerana MRJP1 eucaryon expression product for promoting cell growth. The method is as follows: firstly, construction of a Bac-to-Bac/BmNPV rhabdovirus expression system is utilized for obtaining recombinant Bacmid-MRJP1 which is then transfected to silkworm cells; secondly, after recombinant viruses are obtained, silkworm larvas are injected; thirdly, after the silkworm larvas are transfected for 96 hours, haemolymph is collected; fourthly, after purification of an affinity column, the high-purity recombinant MRJP1 is obtained. As shown by SDS-PAGE and Western-blotting analysis, the molecular weight of the expression product is approximately 60kDa; molecules are mainly in the state of dissolution; the expression amount is obviously higher than prokaryotic expression. The method establishes certain foundation for industrialized production of the MRJP1 and is hopeful to be applied in the fields such as biomedicine and so on for serving the human health.

A combined n-shaped queen-isolating nest door is composed of an n-shaped queen-isolating hole (1), a worker bee one-way queen-isolating hole (2), a deformed channel (3), a nest door foot-pedal (4) and a nest door cover-plate (5). The n-shaped queen-isolating nest door has the following advantages: pollen will not fall when field bees pass by; the nest door can be used for isolating the queen bee and preventing natural enemies such as drone, sphingid, hornet and the like from entering the box; the nest door has good ventilation when closed; field bees do not leave during transitions and steal can be prevented; the queen bee can freely come in and out so as to raise collecting amounts of field bees; the nest door is multifunctional and multipurpose, is convenient to use, and can be adopted to minimize labor intensity of bee keepers, increase earnings of bee keepers and protect Apis cerana cerana.

The invention discloses a two-bee-specie dual queen mixed group construction, and mixed royal jelly and a production method thereof. The two-bee-specie dual queen mixed group construction method includes: S1, selecting an Apis mellifera group or an Apis cerana group as an original bee group; and selecting an Apis cerana comb adjustment group and an Apis mellifera comb adjustment group; S2, putting a sealed sub comb to the original bee group, and allowing bees in the selected sealed sub comb and a sealed sub comb of the original bee group to naturally fly out of the combs; S3, adjusting the proportion of the number of Apis cerana worker bee individuals and the number of Apis mellifera worker bee individuals; and S4, putting an Apis cerana queen and an Apis mellifera queen to the whole bee group, and acquiring an Apis cerana and Apis mellifera mixed group. The Apis cerana and Apis mellifera mixed group can be effectively established, and has two queens of the two bee species; a strong group can be maintained for long time, and respective advantage roles of the Apis cerana and the Apis mellifera can be fully played; various kinds of mixed royal jelly can be produced, the production efficiency of the mixed royal jelly can be improved, and the quality of the mixed royal jelly is ensured.

The invention discloses a yeast expression method for recombining a major protein AccMRJP1 of apis cerana royal jelly and product application. The method comprises the following steps of: screening out a clone containing the full length of an AccMRJP1 gene from an apis cerana brain cDNA library by utilizing a genetic engineering means; obtaining a target gene through PCR (Polymerase Chain Reaction) amplification; optimizing the gene to be linked to an Escherichia coli-yeast shuttle vector pPTC9k; linearizing and introducing to a yeast cell; culturing the yeast cell under the condition of inducing AccMRJP1 expression to finally obtain an expression product; and purifying through affinity chromatograph to obtain a product with the same glycosylation degree and biological activity with natural proteins. The recombined AccMRJP1 obtained in the invention can be used for vitro serum-free culture of passage and primary insects as well as human and animal cells, eliminates the risk of numerous unknown cell factors, such as polluted exogenous viruses and pathogenic factors in blood serum and the interference of antigen, antibody, hormone, and the like, and has wide application in the fields, such as life sciences, medicine, biomedicine, and the like.

The invention discloses a construction method and identification application of fingerprint spectra of natural apis cerana honey of lychee flowers and natural apis mellifera honey of lychee flowers. GC/MS fingerprint spectra of the natural apis cerana honey of lychee flowers and natural apis mellifera honey of lychee flowers are separately constructed by a headspace solid-phase microextraction-gaschromatography-mass spectrometry method and a traditional Chinese medicine fingerprint similarity evaluation system; and it determines that the GC/MS fingerprint spectrum of the natural apis cerana honey of lychee flowers contains 19 common feature volatile components, and the GC/MS fingerprint spectrum of the natural apis mellifera honey of lychee flowers contains 16 common feature volatile components. By comparison of similarity and chemical composition difference of a total ion chromatogram of a to-be-detected natural honey sample of lychee flowers and a constructed GC/MC fingerprint spectrum, the natural apis cerana honey of lychee flowers and the natural apis mellifera honey of lychee flowers are accurately distinguished and identified. The method has the characteristics of small useamount of the sample, high characteristics and reliable result, the problem that the natural apis cerana honey of lychee flowers and the natural apis mellifera honey of lychee flowers in markets aredifficult to distinguish is solved quite beneficially, and the social benefit is quite obvious.