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171 results about "Apis cerana" patented technology
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Apis cerana, the eastern honey bee or the Asiatic honey bee, is a species of honey bee native to southern, southeastern, and eastern Asia. This species is the sister species of Apis koschevnikovi and both are in the same subgenus as the western (European) honey bee, Apis mellifera. A. cerana is known to live sympatrically along with Apis koschevnikovi within the same geographic location. Apis cerana colonies are known for building nests consisting of multiple combs in cavities containing a small entrance, presumably for defense against invasion by individuals of another nest. The diet of this honey bee species consists mostly of pollen and nectar, or honey. Moreover, Apis cerana is known for its highly social behavior, reflective of its classification as a type of honey bee.
Multi-layer overlaying circular frameless apis cerana cerana beehive
InactiveCN102613110AGather sporadic honey sourcesStrong resistance to bee mitesBeehivesApis ceranaEngineering
A multi-layer overlaying circular frameless apis cerana cerana beehive capable of greatly reducing labor capacity and producing pure primitive ecological apis cerana cerana honey is an integral beehive formed by overlaying a plurality of circular following beehives on a circular base plate machined with a concave natural deslagging slope surface, a deslagging hole, a nest entrance and a feeding hole, placing four metal pads of the following beehives on each following beehive, covering the top following beehive with a circular beehive cover with a vent hole, and vertically penetrating and connecting the base plate and connection holes of a four-corner connection clamp on the box cover through four L-shaped reinforcing bars. Therefore, the apis cerana cerana group can naturally form a sphere apis cerana cerana bulk in the beehives, and nests from the top down; and if the stored honey at the uppermost layer is mature, sealed and covered, the four L-shaped reinforcing bars and the corresponding metal pads are detached, the following beehive at the uppermost layer is transversely cut to take out the honey, and then is additionally arranged on the base plate at the bottom layer; and the survival and propagation of the apis cerana cerana after the honey is taken out are not influenced, the output and quality of apis cerana cerana honey are improved, and the multi-layer overlaying circular frameless apis cerana cerana beehive has a vital significance for apis cerana cerana protection.
Owner:张诚
Artificial cultivation method of apis cerana queen
Artificial cultivation method of apis cerana queen, first preparing artifical stylobate of apis mellifera, and choosing a group of apis mellifera queen as feeding group for larva of apis cerana queen; putting the artifical stylobate of apis mellifera into a selected feeding group of apis mellifera group, taking the artifical stylobate out after cleaned by worker bee of the apis mellifera, transfering larva of worker bee in mother apis cerana group that is in need of cultivating queen to the artifical stylobate; at last putting the artifical stylobate into generation box of the selected apis mellifera feeding group for feeding larva of apis cerana queen, after 11 to 12 days the apis cerana queen stylobate is mature, taking out the apis cerana queen stylobate before the virgin queen going out the room, introducing the virgin queen to apis cerana mating group for following mating, so as to become a spawning queen. The superiority of the invention is: obtaining large amount of mature apis cerana queen stylobate or virgin queen for one time; because of adequent apis mellifera bee milk, bigger and stronger virgin queen can be cultivated; apis mellifera and apis cerana of different species can generates nutritious heterosis which provides important meaning for apis cerana species cultivation.
Owner:慈溪市畜牧兽医技术推广中心
Apis cerana cerana health care feed
ActiveCN102356821AImprove palatabilityWide variety of sourcesAnimal feeding stuffAccessory food factorsApis ceranaPotential fecundity
The invention discloses an apis cerana cerana health care feed, which is composed of the following components by total mass fraction 100%, bee pollen 66%-78%, protein power 21%-33%, astragalus polysachain (content 40%) 0.2%-1.5% and riboflavin 0.3%. According to experiments, apis cerana cerana swarms fed by the apis cerana cerana health care feed are good in palatability, and bees like to feed. Compared with swarms fed by common protein feed (defatted soybean powder), fecundity is improved by more than 42.6%, and group potential is improved by 27%, morbidity is reduced by 39%. Compared with apis cerana cerana swarms fed by high-quality bee pollen feed, group potential is improved by 6%, morbidity is reduced by 25%, and effect is obvious. Therefore, the apis cerana cerana health care feed is good in palatability for the apis cerana cerana, bee like to feed, group potential fecundity of the apis cerana cerana can he increased, group potential can be improved, and morbidity can be reduced. Moreover, the apis cerana cerana health care feed is wide in source and simple to prepare and can be widely applied to industrialized culture of bees.
Owner:INST OF ZOOLOGY GUANGDONG ACAD OF SCI
Apis cerana royal jelly antibacterial peptide AccRoyalisin gene and encoded polypeptide thereof and application thereof
InactiveCN101705231AGood inhibitory effectAvoid pollutionAntibacterial agentsPeptide/protein ingredientsEscherichia coliApis cerana
The invention discloses an Apis cerana royal jelly antibacterial peptide AccRoyalisin gene and an encoded polypeptide thereof and application thereof. The gene has a DNA sequence shown as SEQ ID No.4; and the encoded protein of the gene has an amino acid sequence shown as SEQ ID No.5. The antibacterial peptide gene can establish a high-expression genetic engineering strain by building an Escherichia coli expression vector, and performing producing and separating and purifying on a recombinant product through induction expression of the fermentation engineering. The recombinant product thereof has an obvious inhibiting effect on a plurality of gram positive bacteria, such as bacillus subtilis, staphylococcus aureus and micrococcus flavus and the like, can be used for preserving and refreshing foods, in particular for preventing food-born bacterial contamination, is harmless to human body and animals, can serve as a substitute of antibiotics and chemical preservatives and is also suitable for preventing and controlling bred animal bacterial diseases. The Apis cerana royal jelly antibacterial peptide AccRoyalisin gene and the encoded polypeptide thereof lay a foundation for the development, the massive industrial production and the application of the Royalisin.
Owner:ZHEJIANG UNIV
Method for measuring content of apis mellifera MRJP1 protein in honey by using liquid chromatography-tandem mass spectrometry
InactiveCN110596262AMaintain healthy developmentStrong specificityComponent separationBiological testingApis ceranaProtein mass spectrometry
The invention provides a method for measuring the content of apis mellifera MRJP1 protein (major royal jelly protein 1) in honey by using liquid chromatography-tandem mass spectrometry. The method comprises the steps of selecting characteristic peptides, preparing a standard curve, pre-treating a sample, performing liquid chromatography separation, and detecting and quantifying tandem mass spectrometry. The method for detecting the content of apis mellifera MRJP1 in honey provided by the invention has the advantages of strong specificity, high sensitivity, good accuracy and precision, and is suitable for accurate quantification of apis mellifera MRJP1 in honey. Since the content of MRJP1 in honey is relatively constant, the method can be used for evaluating the authenticity of honey basedon the content and assisting in identifying whether honey is adulterated or not. In addition, apis cerana honey does not contain apis mellifera MRJP1 and thus can be used for identifying whether apismellifera is mixed in the apis cerana honey. The method is of great significance for maintaining the healthy development of the honey consumption industry and the rights and interests of honey consumers.
Owner:BEE RES INST CHINESE ACAD OF AGRI SCI
Foundation-free section honey production method of Apis ceranas
The invention relates to a foundation-free section honey production method of Apis ceranas, which replaces section honey lattices by special section honey frames, replaces queen separation plates by separation plates and replaces purpose-made shallow boxes by standard boxes. The section honey production method of the Apis ceranas comprises the flowing steps of: manufacture of the special section honey frames and the separation plates, obtaining of non-husks natural spleen bases, fixation of spleen bases, fixation of the section honey frames, production of the section honey of the Apis ceranas, section honey cutting and section honey package. The production method is simple and practical, reduces the production cost and has low cost. The section honey has better quality and richer fragrance. The honey yield can be increased because no queen plate is adopted. The production method changes complexity into simplicity, reduces the labor intensity, has low technical requirements, is easy to learn and master by beekeepers and has low labor intensity in the production process. Strong colony is easy to maintain because the space of honey boxes is increased. The nest insect harm is reduced.
Owner:ZUNYI NORMAL COLLEGE
ELISA detection kit for detecting Apis cerana Smith honey and Apis mellifera honey
ActiveCN110376384AAccurate detectionMeet the needs of fast and convenient detectionNucleic acid vectorBiological testingApis ceranaProtein target
The invention provides an ELISA detection kit for detecting Apis cerana Smith honey and Apis mellifera honey. The MRJP2(major royal jelly protein 2) is selected as the target protein for Apis cerana Smith honey and Apis mellifera honey detection, the Apis cerana Smith MRJP2 and the Apis mellifera MRJP2 are obtained through a Prokaryotic expression purification, and preparing and purifying to obtain the specificity and identity antibody of the Apis cerana Smith MRJP2 and the Apis mellifera MRJP2, and the Apis cerana Smith honey and the Apis mellifera detection kit is developed by using a doubleantibody sandwich method. The ELISA detection kit disclosed by the invention has high sensitivity, the developing condition can be observed through the naked eyes, 1% of Apis mellifera honey doping in the Apis cerana Smith can be identified within 45min, the convenient and fast detection requirement is satisfied, and the detection kit is a reliable tool for Apis cerana Smith honey and Apis mellifera honey detection.
Owner:ZHEJIANG UNIV
Method for identifying honey of Apis cerana and honey of Apis mellifera
InactiveCN108362784AAccurate identificationTo achieve the purpose of identificationComponent separationAlkaneApis cerana
The invention provides a method for identifying the honey of Apis cerana and the honey of Apis mellifera. The method achieves the purpose of identifying the two kinds of honey based on the differenceof alkane components in the two kinds of honey. Honey samples are extracted with petroleum ether and then detected via gas chromatography-mass spectrometry; and detection results show that the alkanecomponents of the honey of Apis cerana and the honey of Apis mellifera are different, the honey of Apis cerana contains C37 alkanes, and the honey of Apis mellifera contains C31 alkanes. It is confirmed that the component difference is only related to bee species after detection and analysis of a large number of samples, and the influence of plant sources on identification is excluded. The differential alkane components are stable in honey and are not affected by concentration processing and shelf life. The method of the invention is reasonable in design, convenient, quick and accurate in identification, and is applicable to both the identification of the sources of raw honey and commercial bee species and the identification of the honey of Apis cerana blended with a certain proportion ofthe honey of Apis mellifera.
Owner:ZHEJIANG UNIV
Method for identifying apis cerana honey and apis mellifera honey
InactiveCN105548321AAccurate identificationSimple designMaterial analysis by electric/magnetic meansApis ceranaProtein target
The invention provides a method for identifying apis cerana honey and apis mellifera honey, which achieves the purpose of identifying the apis cerana honey and the apis mellifera honey according to the specific band difference between the proteins of the apis cerana honey and the apis mellifera honey. After an apis cerana honey sample is treated, protein electrophoresis is conducted, the apis cerana honey has a 15.0KDa protein band, the apis mellifera honey has a 16.5KDa protein band, and a mixed honey sample of the both has both the 15.0KDa protein band and the 16.5KDa protein band. By differentiating the specific protein bands of the different honeys to achieve the purpose of identification, the method can accurately identify the apis cerana honey and the apis mellifera honey. In the method disclosed by the invention, the target proteins stably exist in the apis cerana honey and the apis mellifera honey, not affected by honey resources, concentration processing and shelf life. The design of the method disclosed by the invention is reasonable and simple, instruments are common, cost is low, identification is accurate, and the method is also suitable for identifying a certain proportion of apis mellifera honey mixed in the apis cerana honey.
Owner:ZHEJIANG UNIV
Method for determining apis cerana Fabricius MRJP1 protein content in honey by utilizing liquid chromatography tandem mass spectrum
InactiveCN110596261AMaintain healthy developmentStrong specificityComponent separationBiological testingBiotechnologyChromatographic separation
The invention provides a method for determining apis cerana Fabricius MRJP1 protein content (major royal jelly protein 1) in honey by utilizing liquid chromatography tandem mass spectrum. The method comprises the following steps: selecting characteristic peptide fragment, manufacturing a standard curve, pretreating the sample, performing liquid chromatographic separation and tandem mass spectrum detection quantitation and like steps. The detection method of apis cerana Fabricius MRJP1 content in honey has high accuracy, precision and sensitivity, and strong in stability, and suitable for the accurate quantitation of the apis cerana Fabricius MRJP1. The method further can be used for the truth evaluation of the honey, thereby assisting to identify whether the honey is adulterated, and is ofgreat significance for maintaining the healthy development of the honey consumption industry and the rights and interest of the honey consumer.
Owner:BEE RES INST CHINESE ACAD OF AGRI SCI
Method for breeding bees using roxburgh rose as flower source
InactiveCN106234316AIncrease productionImprove egg production performanceAnimal feeding stuffAccessory food factorsApis ceranaDisease
The invention relates to a method for breeding bees using roxburgh rose as flower source comprising following steps: (1) selecting breeding fields; (2) preparing beehives; (3) arranging the beehives; (4) breeding bees (5) fine selecting bee colonies; (6) cleaning the honey; (7) promoting collecting honey. According to the method for breeding bees using roxburgh rose as flower source, Apis cerana is selected as seedling bees, through artificial cultivation, queen bees are grown up; the bees are bred with special bee forages which can increase the vitality of worker bees, the egg production capacity of queen bees and disease resistance of bees so that the bees can grow rapidly, fecundity is high, the bee colonies are enhanced and honey production is increased. More honey can be collected during the blooming period of roxburgh rose, wherein the honey has a variety of medicinal and health benefits, can enrich the variety and quality of honey to improve the economic benefits of beekeeping.
Owner:仁怀市长干山土蜂养殖专业合作社
Apis cerana queen breeding method
The invention discloses an apis cerana queen breeding method and belongs to bee queen breeding methods. By means of the apis cerana queen breeding method, a bred queen can be large in size, strong, meek in temperament and high in egg production capacity. Bowl-shaped queen cells made of bee wax are fixed to a honeycomb frame, and a queen breeding frame is obtained; the queen breeding frame is put in a queen breeding apis cerana swarm, and worker bees are made to clean the queen cells; the inner walls of the queen cells are brushed with fresh bee milk, eggs of other apis cerana swarms are implanted into the queen cells, and the queen breeding frame is coated with apis mellifera honey in a dipping mode and then put back to the queen breeding apis cerana swarm; the queen breeding frame is taken out and put into an apis mellifera swarm for nursing for 12 hours, then the queen breeding frame is taken out and put back to the queen breeding apis cerana swarm again for breeding for 5-7 days, and a mature queen embryo is obtained; a virgin queen can be hatched on the 9th or 10th day. The queen bred through the method is large in size, strong, high in disease resistance and high in egg production capacity, the bee swarm is meek in temperament, and the bee robbing or honey stealing phenomenon can be avoided even bee swarms are away by 10 cm, and the apis cerana queen breeding method is used for breeding apis cerana.
Owner:CHANGSHUN CHICHENG PLANTING & BREEDING FARMER SPECIALIZED COOP
Fused heterocyclic compounds and application thereof
ActiveCN108003162AGood killing effectEnhance killing activityBiocideOrganic chemistryApis ceranaChemical compound
The present invention discloses fused heterocyclic compounds as well as optical isomers, cis-trans isomers or agrochemically acceptable salts thereof and an application of a composition. The fused heterocyclic compounds have a structure shown in a formula I in the description. The fused heterocyclic compounds as well as the optical isomers, the cis-trans isomers or the agrochemically acceptable salts thereof provided by the present invention have high killing activity against agricultural and forestry pests, animal parasitic pests, sanitary pests and the like, delayed effects on invasive solenopsis invicta, and lower toxicity to environmental organisms such as apis cerana and bombyx mori.
Owner:SOUTH CHINA AGRI UNIV
Method for breeding special pollination apis cerana
InactiveCN103283683AImprove pollination efficiencyQuality improvementAnimal husbandryApis ceranaGreenhouse
The invention discloses a method for breeding special pollination apis cerana. The method includes steps of 1), selecting breeding materials: selecting a high-quality and high-production apis cerana species from a first-grade breeding apis cerana field of the Zhejiang province, introducing high-quality apis cerana from the Hainan province, and combining the high-quality and high-production apis cerana species, the high-quality apis cerana and local high-quality, high-production and gentle apis cerana difficult in swarming with one another to obtain the breeding materials; 2), performing three-way cross breeding: utilizing the apis cerana of the Hainan province as male parents and Fuchide apis cerana as female parents, performing cross breeding and directive breeding on the male parents and the female parents by an artificial insemination mode, selecting a high-quality, high-production, high-temperature-resistant, high-moisture-resistant and weak-swarming apis cerana species as breeding female parents for a next apis cerana generation, selecting local high-quality apis cerana as male parents and performing cross breeding on the local high-quality apis cerana and the cross breeding female parents; 3), locked breeding: creating 60 high-quality apis cerana colonies with three female parent characteristics, and enabling the high-quality apis cerana colonies to perform natural mating under an isolated natural condition. The method has the advantages that various technical bottlenecks in the aspect of bee pollination can be solved for greenhouse fruit and vegetable growers, and the bee pollination efficiency and the bee pollination quality are improved for greenhouses.
Owner:LANXI HUAYAN MIFANG AGRI SCI & TECH CO LTD
Method for improving productivity of Apis cerana cerana by organizing strong colonies through mode of putting three queen bees in the same beehive
The invention discloses a method for improving the productivity of Apis cerana cerana by organizing strong colonies through a mode of putting three queen bees in the same beehive. The method for improving the productivity of the Apis cerana cerana by organizing the strong colonies through the mode of putting the three queen bees in the same beehive is characterized in that the three queen bees are put into the same beehive and isolated through queen excluders, but colonies are not isolated, and one of the queen bees is prevented from oviposition alternately and is in a state of rest; then the three queen bees have a rest alternately and regularly and achieve oviposition alternately to achieve the optimum state of oviposition and the highest honey yield. According to the method for improving the productivity of the Apis cerana cerana by organizing the strong colonies through the mode of putting the three queen bees in the same beehive, the aim that 17 combs of the strong colonies can obtain a high yield of 90-12kg per strong colony per year can be maintained through the simplest method and the lowest cost. The method for improving the productivity of the Apis cerana cerana by organizing the strong colonies through the mode of putting the three queen bees in the same beehive is very ideal in effect, needs little investment and becomes effective very quickly.
Owner:青海青藏华峰中蜂蜂业有限公司
Steel-wood structured Apis cerana Smith activity frame
The invention relates to a firm and durable steel-wood structured Apis cerana Smith activity frame which can enable Apis cerana Smith to go up and down a super smoothly, wherein the steel-wood structured Apis cerana Smith activity frame is formed by combining an upper concave metal beam, a lower concave metal beam, two wood edge strips and a fine iron wire by using iron nails; and the upper beam and the lower beam are about 1 mm in thickness respectively. According to the invention, with the all-metal Apis cerana Smith bee-way queen excluder (patent number: 201210142959.x) disclosed by the invention, separation of brood rearing and honey producing of Apis cerana Smith is realized; quality and yield of Apis cerana Smith honey are increased; and the problem of raising Apis cerana Smith by using the super is solved.
Owner:张诚
Elite breeding method for apis cerana in southern area of China
Owner:湖南蜜蜂哥哥蜂业有限公司
Wall-hung D-shaped frameless Apis cerana hive
A wall-hung D-shaped frameless Apis cerana hive is characterized in that a D-shaped base ring is placed on a D-shaped metal bracket, a concave base plate is mounted below the D-shaped metal bracket, 4-6 D-shaped supers are stacked on the D-shaped base ring, a D-shaped cover covers the supers, and gaps between the D-shaped supers and gaps between the D-shaped supers and the cover are sealed by bee wax. The wall-hung D-shaped frameless Apis cerana hive has the advantages that Apis cerana is allowed to form spherical clusters, viewing, swarming and honey taking are convenient, and honey is prevented from secondary pollution; the Apis cerana hive which accords to natural living habits of Apis cerana is suitable for Apis cerana beekeeping in permanent apiary in scattered families in villages and modern villas, the Apis cerana hive combines advantages of novel Apis cerana hives and traditional Apis cerana hives and overcomes defects of the novel Apis cerana hives and the traditional Apis cerana hives, the Apis cerana hive is free of usual management and feeding, and the honey produced is purely natural, original, and various in kinds; the wall-hung D-shaped frameless Apis cerana hive is simple to mount, convenient to assemble, firm and durable, and long in service life.
Owner:CHEGNGDU CHENG KE TROTHER APICULTURE COMPANY
Application of 3-amino-2-naphthoic acid in identification of apis cerana honey and apis mellifera honey
ActiveCN111398499AAccurate identificationProtection of legal rightsComponent separationBiotechnologyApis cerana
The invention relates to the field of food detection, and particularly discloses an application of 3-amino-2-naphthoic acid in identification of apis cerana honey and apis mellifera honey. Meanwhile,the invention provides a method for identifying the apis cerana honey and the apis mellifera honey by taking the content of the 3-amino-2-naphthoic acid as an identification basis. The method comprises the steps of: if the content of 3-amino-2-naphthoic acid in a honey sample is between 17.59-620.01 mu g / kg, judging that the honey sample is apis cerana honey; and if the content of 3-amino-2-naphthoic acid in the honey sample is between 0.04-12.00 mu g / kg, judging that the honey sample is apis mellifera honey. The method provided by the invention is simple, convenient, accurate and convenient to operate and popularize, and has important practical significance for protecting legal rights and interests of honey consumers and maintaining healthy development of the honey consumption industry.
Owner:BEE RES INST CHINESE ACAD OF AGRI SCI
Production technique for comb foundation-free honey of Apis cerana
InactiveCN104351084AIncrease the number ofImprove secretion capacityBeehivesHoney collectionApis ceranaQueen bee
The invention provides a production technique for comb foundation-free honey of Apis cerana. The production technique comprises the following steps: manufacturing a beehive with two queen bees in one beehive; replacing comb honey bee lattices in one month before each blooming period, feeding honey bees with a nutritional feed in 5-30 days before the blooming period, adopting sealing plates to seal comb honey bee nests on the comb honey bee lattices during the process, and timely taking out to place into a sterilized thermostat container for storage stopping feeding the honey bees with the nutritional feed within 5 days before the blooming period; when the blooming period begins, examining the sealing condition of the comb honey bees at any time, and replacing the comb honey bee lattices provided with sealed comb honey by the comb honey bee lattices stored in the thermostat container. The production technique realizes two queen bees in one beehive through the unique beehive, and increases the amount of worker bees in the beehive, so that the speed of building nests and collecting honey of the Apis cerana is higher; meanwhile, by feeding the Apis cerana with the nutritional feed, integral health of the honey bees is improved, the secretion capability of the worker bees is enhanced, and the speed of building nests is accelerated; by the adoption of the production technique, the speed of producing comb honey is high, and the amount of comb honey is increased.
Owner:仁怀市土蜂养殖专业合作社
Method for distinguishing Apis cerana cerana honey from Apis mellifera ligustica honey
ActiveCN112595784AProtection of legitimate rights and interestsSimple and fast operationComponent separationApis ceranaHydrocotyle bowlesioides
The invention relates to a method for distinguishing distinguishing Apis cerana cerana honey from Apis mellifera ligustica honey. The method comprises the steps of (1) weighing a detected amount of honey sample to be detected; (2) detecting volatile hydrocarbon compounds in the honey to be detected by using HS-SPME-GC-MS to obtain a detection spectrogram, and quantifying Cis-9-Tricosene, (C9T), isomers thereof, namely, Iso-9-Tricosene, Cis-9-Pentacosene, (C9P) and an isomer Iso-9-Pentacosene in the honey sample to be detected according to the spectrogram; and (3) identifying the Apis cerana cerana honey and Apis mellifera ligustica honey according to the contents of C9T and C9P in the honey and the proportions of C9T and C9P to respective isomers.
Owner:秦皇岛海关技术中心
Method for rapidly reproducing Apis cerana by using movable frame capable of overturning lower part of honeycomb
The invention relates to a method for rapidly reproducing Apis cerana by using a movable frame capable of overturning a lower part of a honeycomb. Beewax connected to the honey comb, on the upper part of a circular frame ring is cut while the area of honey stored in the upper part of the circular frame ring, of the Apis cerana is more than 1 / 3 of the circular frame ring; the circular frame ring is overturned at an angle of 180 degrees, the honey stored in the circular fame ring faces downward, and eggs, larva and pupae in the circular frame ring face upward. The method meets a natural life habit of the Apis cerana, can expand the egg laying range of a queen bee, can rapidly reproduce the Apis cerana, is conductive to protection and development of the Apis cerana, and can breed Apis mellifera and other bee species.
Owner:CHEGNGDU CHENG KE TROTHER APICULTURE COMPANY
Smell activator screening method based on apis cerana odorant binding protein
The invention discloses a plant fragrance smell activator formula screening method based on apis cerana odorant binding protein. The method comprises the following process steps: 1) extracting working bee antenna total RNA (Ribonucleic Acid) of apis cerana; 2) designing an apis cerana odorant binding protein gene primer, and acquiring the full length of an apis cerana odorant binding protein gene through RT-PCR (Reverse Transcription-Polymerase Chain Reaction); 3) building a prokaryotic expression vector of the apis cerana odorant binding protein gene; 4) inducing recombinant expression of the apis cerana odorant binding protein through IPTG (isopropyl-beta-d-thiogalactoside), and performing purification through a Ni Sepharose FF affinity column; 5) acquiring a combined response spectrum of the apis cerana odorant binding protein and plant fragrance smell through a competitive fluorescent combination method, and determining the combined response spectrum as the plant fragrance smell activator suitable for the apis cerana when the dissociation constant KD is lower than 20mummol / L.
Owner:CHINA JILIANG UNIV
Method for preparing MRJP1 eucaryon expression products of China honey bee for promoting growth of cells
InactiveCN101289664APromote growthImprove securityTissue cultureGenetic engineeringApis ceranaBiotechnology
The invention discloses a method for preparing an Apis cerana MRJP1 eucaryon expression product for promoting cell growth. The method is as follows: firstly, construction of a Bac-to-Bac / BmNPV rhabdovirus expression system is utilized for obtaining recombinant Bacmid-MRJP1 which is then transfected to silkworm cells; secondly, after recombinant viruses are obtained, silkworm larvas are injected; thirdly, after the silkworm larvas are transfected for 96 hours, haemolymph is collected; fourthly, after purification of an affinity column, the high-purity recombinant MRJP1 is obtained. As shown by SDS-PAGE and Western-blotting analysis, the molecular weight of the expression product is approximately 60kDa; molecules are mainly in the state of dissolution; the expression amount is obviously higher than prokaryotic expression. The method establishes certain foundation for industrialized production of the MRJP1 and is hopeful to be applied in the fields such as biomedicine and so on for serving the human health.
Owner:ZHEJIANG UNIV
Combined n-type queen-isolating nest door
A combined n-shaped queen-isolating nest door is composed of an n-shaped queen-isolating hole (1), a worker bee one-way queen-isolating hole (2), a deformed channel (3), a nest door foot-pedal (4) and a nest door cover-plate (5). The n-shaped queen-isolating nest door has the following advantages: pollen will not fall when field bees pass by; the nest door can be used for isolating the queen bee and preventing natural enemies such as drone, sphingid, hornet and the like from entering the box; the nest door has good ventilation when closed; field bees do not leave during transitions and steal can be prevented; the queen bee can freely come in and out so as to raise collecting amounts of field bees; the nest door is multifunctional and multipurpose, is convenient to use, and can be adopted to minimize labor intensity of bee keepers, increase earnings of bee keepers and protect Apis cerana cerana.
Owner:陈日宝
Two-bee-specie dual queen mixed group construction, mixed royal jelly and production method thereof
ActiveCN107047486AEffective establishmentIncrease productivityAnimal husbandryFood scienceApis ceranaMixed group
The invention discloses a two-bee-specie dual queen mixed group construction, and mixed royal jelly and a production method thereof. The two-bee-specie dual queen mixed group construction method includes: S1, selecting an Apis mellifera group or an Apis cerana group as an original bee group; and selecting an Apis cerana comb adjustment group and an Apis mellifera comb adjustment group; S2, putting a sealed sub comb to the original bee group, and allowing bees in the selected sealed sub comb and a sealed sub comb of the original bee group to naturally fly out of the combs; S3, adjusting the proportion of the number of Apis cerana worker bee individuals and the number of Apis mellifera worker bee individuals; and S4, putting an Apis cerana queen and an Apis mellifera queen to the whole bee group, and acquiring an Apis cerana and Apis mellifera mixed group. The Apis cerana and Apis mellifera mixed group can be effectively established, and has two queens of the two bee species; a strong group can be maintained for long time, and respective advantage roles of the Apis cerana and the Apis mellifera can be fully played; various kinds of mixed royal jelly can be produced, the production efficiency of the mixed royal jelly can be improved, and the quality of the mixed royal jelly is ensured.
Owner:JIANGXI AGRICULTURAL UNIVERSITY
Beehive and method for increasing apis cerana comb honey yield by beehive
ActiveCN109548706AReduced insulation performanceReduce going outAccessory food factorsBeehivesApis ceranaClamp connection
The invention discloses a beehive in the technical field of apis cerana breeding. The beehive comprises a base and a beehive body formed by a front plate, a rear plate, a bottom plate, a cover and side plates. A thermal preserving layer fitting the bottom plate is in slide connection between the base and the bottom plate; two partition plate groups are slidably connected to the upper surface of the base; each partition plate group comprises a thermal insulating plate, a thermal preserving plate and a retaining plate in sequential lamination, wherein one side of the retaining plate fits the corresponding side plate; grooves are uniformly distributed in one side of each side plate; a two-shaft motor is arranged in the base, two output shafts of the two-shaft motor are fixedly connected withrotating shafts in rotational connection with two gears, and the three gears are in clamping connection with the rotating shafts and meshed with the thermal insulating plates, the thermal preserving plates and the retaining plates through meshing teeth; a thermal preserving cover is in slide connection in a sleeve which is arranged on the base, the thermal preserving cover is positioned right above the cover, a connection rod is fixedly connected to the thermal preserving cover, and a rack meshed with one of the gears is hinged to the connection rod. By adoption of the beehive for apis ceranabreeding, the comb honey yield can be remarkably increased.
Owner:GUIZHOU WUCHUAN YONGHUI APICULTURE TECH CO LTD
Yeast expression method for recombining major protein AccMRJP1 of apis cerana royal jelly and product application
The invention discloses a yeast expression method for recombining a major protein AccMRJP1 of apis cerana royal jelly and product application. The method comprises the following steps of: screening out a clone containing the full length of an AccMRJP1 gene from an apis cerana brain cDNA library by utilizing a genetic engineering means; obtaining a target gene through PCR (Polymerase Chain Reaction) amplification; optimizing the gene to be linked to an Escherichia coli-yeast shuttle vector pPTC9k; linearizing and introducing to a yeast cell; culturing the yeast cell under the condition of inducing AccMRJP1 expression to finally obtain an expression product; and purifying through affinity chromatograph to obtain a product with the same glycosylation degree and biological activity with natural proteins. The recombined AccMRJP1 obtained in the invention can be used for vitro serum-free culture of passage and primary insects as well as human and animal cells, eliminates the risk of numerous unknown cell factors, such as polluted exogenous viruses and pathogenic factors in blood serum and the interference of antigen, antibody, hormone, and the like, and has wide application in the fields, such as life sciences, medicine, biomedicine, and the like.
Owner:ZHEJIANG UNIV
Construction method and identification application of fingerprint spectra of natural apis cerana honey of lychee flowers and natural apis mellifera honey of lychee flowers
ActiveCN109470779AFix the problem of indistinguishableHigh detection sensitivityComponent separationApis ceranaSocial benefits
Owner:INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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