2 results about "Sepharose" patented technology

The application provides a detection reagent for frozen semen of Huai pig, and first acquires the raw semen, and then carries out the following steps: ammonium sulfate fractionation precipitation, DEAE cellulose filter gel column chromatography, CM Sepharose FastFlow gel chromatography and Sephadex G-100 chromatography to separate and purify the NAGase of the semen of the Huai pig, and monoclonal antibodies JN21 and JN37 are prepared by taking the NAGase as an antigen, and the monoclonal antibodies can specifically recognize the NAGase of the Huai pig. The prepared double-antibody sandwich ELISA kit can rapidly and quantitatively detect the NAGase content in the semen of the Huai pig, and provides a new thought for Huai pig breeding and purification and rejuvenation. The kit is convenient to operate, low in use cost, good in repeatability and suitable for being widely applied.

This invention belongs to the field of biotechnology. Specifically, it discloses a method for preparing recombinant protein using rice seeds. The method involves dehulling rice seeds to obtain rice flour, which is then enzymatically hydrolyzed using cellulase and neutral protease in a buffer solution. This process efficiently hydrolyzes the cellulose in the rice cell wall and some impurities. The buffer solution reduces protein salting out and increases the dissolution rate of recombinant human lactoferrin. Activated carbon is used to initially adsorb and remove impurities such as natural pigments and phenolic substances from the rice seeds. The mixture is then subjected to chromatography using CM Sepharose FF, SP Sepharose XL, and CM Sepharose FF columns, effectively improving the purity and recovery rate of the recombinant human lactoferrin. Simultaneously, the enzymatic hydrolysis and fractionation chromatography effectively reduce the iron saturation in the recombinant human lactoferrin.