1 results about "Sepharose" patented technology

This invention belongs to the field of biotechnology. Specifically, it discloses a method for preparing recombinant protein using rice seeds. The method involves dehulling rice seeds to obtain rice flour, which is then enzymatically hydrolyzed using cellulase and neutral protease in a buffer solution. This process efficiently hydrolyzes the cellulose in the rice cell wall and some impurities. The buffer solution reduces protein salting out and increases the dissolution rate of recombinant human lactoferrin. Activated carbon is used to initially adsorb and remove impurities such as natural pigments and phenolic substances from the rice seeds. The mixture is then subjected to chromatography using CM Sepharose FF, SP Sepharose XL, and CM Sepharose FF columns, effectively improving the purity and recovery rate of the recombinant human lactoferrin. Simultaneously, the enzymatic hydrolysis and fractionation chromatography effectively reduce the iron saturation in the recombinant human lactoferrin.