294 results about "Citrus fruit" patented technology

The invention discloses an application of a CsHCT1 gene in increasing the content of citrus flavonoids, belongs to the technical field of agricultural biological genetic engineering, and provides an application of the CsHCT1 gene in increasing the content of citrus flavonoids, and the nucleotide sequence of the CsHCT1 gene is as shown in SEQ ID NO.1. The invention further discloses an application of the CsHCT1 gene in increasing the content of citrus flavonoids. The invention also discloses a method for improving the flavone compound of the citrus peel by using the CsHCT1 gene. The method comprises the following steps: cloning the citrus CsHCT1 gene; a CsHCT1 overexpression vector is constructed; transforming the overexpression vector to agrobacterium tumefaciens to obtain an agrobacterium tumefaciens liquid; and injecting the CsHCT1 gene into the citrus fruit to obtain the CsHCT1 gene overexpressed citrus fruit. A new choice is provided for increasing the flavonoid compounds in the citrus peel, the content of vicenin-2 and / or hesperidin in the citrus peel is effectively increased, and the application has great application value for cultivating new functional citrus varieties and accelerating the breeding process.

The invention belongs to the field of citrus breeding, and discloses application of a citrus CsERF113 gene in regulation and control of plant fruit abscission. It is found for the first time that the CsERF113 gene can induce plant separation layer formation and fruit abscission, which has a great promotion effect on molecular mechanism research of abscission regulation of plants including organs such as fruits and the like; the citrus CsERF113 gene cloned by the invention has a remarkable induction effect on citrus fruit abscission, which is of great significance to cultivation of overwintering non-abscission late-maturing citrus varieties through a gene editing technology.

The invention discloses an indel molecular marker for identifying color and luster of citrus peel in a seedling stage and application of the indel molecular marker. The corresponding sites of the indel molecular marker of the citrus are positioned in chr7: 18, 398, 798-18, 399 and 289 intervals of a chromosome 7 of an SWO3.0 reference genome; the genome DNA of the citrus seedling stage plant is used as a template, the Indel molecular marker is amplified through PCR, the citrus seedling stage plant can be divided into a homozygous insertion type, a homozygous deletion type and a heterozygous type according to a PCR amplification result, and the three types are associated with the color of the citrus fruit for identifying the color of the citrus peel, so that the early screening of the seedling stage target traits of the citrus breeding population is realized, and the early screening of the seedling stage target traits of the citrus breeding population is realized. The breeding period is remarkably shortened, the breeding cost is reduced, and important technical support is provided for citrus fruit color improvement breeding.

The invention discloses an ecological planting method for remarkably improving the amino acid content of citrus, which comprises the following steps: (1) mixing and fermenting pruned citrus branches, weed straws, livestock and poultry manure and indigenous probiotic ferment powder to prepare a basic microbial fertilizer, and adding a calcium magnesium phosphate fertilizer and monopotassium phosphate to prepare a special microbial fertilizer for citrus, applying as a base fertilizer in winter; (2) after citrus pruning, in key phenological periods such as a flower bud period, a young fruit period and a fruit swelling period, an indigenous probiotic enzyme diluent with a specific dilution ratio is used for systematic foliage spraying on a tree body. The indigenous probiotic enzyme contains bacillus amyloliquefaciens, saccharomycetes, active enzymes and a plurality of nutrient elements. The method can significantly increase the total amino acid content of citrus fruits, greatly increase the yield, improve the taste of the fruits, enhance the tree body resistance, reduce the use of chemical pesticides, improve soil, and realize quality improvement, yield increase and green sustainable development of citrus planting.

The invention provides a composition and application thereof in preparation of a fat-reducing product. The composition comprises ginger and green tea composite micro-capsule powder, raspberry ketone, mango concentrated powder, citrus fruit powder and seaweed powder. The composition comprises the following components in parts by weight: 15-22 parts of fresh ginger and green tea composite microcapsule powder, 4-8 parts of mango concentrated powder, 1-5 parts of raspberry ketone, 1-5 parts of citrus fruit powder and 1-5 parts of seaweed powder. The method can solve the problem of large side effects of fat-reducing products in the prior art, and is suitable for the field of functional foods.

The invention discloses a fruit and vegetable grafting wound healing nutrient solution containing polysaccharide complex enzyme and a preparation method, belongs to the technical field of agricultural biochemical preparations, and aims to solve the technical problems of low removal efficiency of necrotic tissues at grafting wounds and high risk of microbial infection. According to the technical scheme, the nutrient solution is characterized by being prepared from the following raw material components in parts by weight: 0.3 to 0.8 part of polysaccharide, 1 to 5 parts of cellulase, 0.5 to 2.5 parts of pectinase, 1 to 5 parts of glycerol and 90 to 100 parts of water. Wherein the pH value of the nutrient solution is adjusted to 5.0-6.5 by using a phosphate buffer agent, the polysaccharide is composed of chitosan and sodium alginate, the mass ratio of the chitosan to the sodium alginate is 1: 0.5-1: 2, the activity of cellulase in the nutrient solution is 100-500 U / mL, and the activity of pectinase in the nutrient solution is 50-250 U / mL. The nutrient solution is mainly used for promoting fruit and vegetable grafting wound healing, and application verifies that the nutrient solution can effectively improve wound healing quality and reduce infection risk in the grafting process of fruits and vegetables such as cucumbers, eggplants, peppers, tomatoes, litchis and oranges, and has a good application effect.

The invention provides an application of a citrus transcription factor CsAGL9 gene and a protein coded by the citrus transcription factor CsAGL9 gene in regulating and controlling the content of citric acid in citrus fruits, the nucleotide sequence of the CsAGL9 gene is shown as SEQ ID NO.1, and the amino acid sequence of the protein is shown as SEQ ID NO.2. The content of citric acid in the fruits can be reduced through overexpression in the oranges; the content of citric acid in fruits can be increased by silencing the gene. It is further verified that the gene promotes the activity of the citrus citric acid transporter CsTCT1. The discovery provides a new gene resource for citrus quality improvement and molecular design breeding, and provides an efficient tool for fruit quality improvement.

The invention discloses a soothing and oil-controlling composition derived from natural plants and application thereof, and belongs to the technical field of daily cosmetics. The composition is formed by compounding an artemisia annua extract, a rehmannia root extract, a calendula extract and a pomelo fruit extract, and the four plant extracts have a synergistic effect, have the effects of repairing, relieving and controlling oil, and can be used for preparing a skin care product special for oil-sensitive skin. The composition provided by the invention can effectively solve the problems of external oil and internal dry, redness and sensitivity, grease accumulation and the like of oily sensitive skin, and meets multiple skin care requirements.

The invention provides a bactrocera minax forked head transcription factor FOXO as well as a coding gene and application thereof, and belongs to the technical field of biological prevention and control of crop pests. A nucleic acid molecule of the citrus fruit fly forked head transcription factor FOXO is cloned, dsRNA for interfering the nucleic acid molecule of the citrus fruit fly coded forked head transcription factor FOXO is synthesized, and the dsRNA is introduced into the citrus fruit fly by using an injection method and a feeding method to carry out RNAi on the citrus fruit fly forked head transcription factor gene FOXO. After the dsRNA is injected or fed to the bactrocera minax, the survival rate, the growth rate, the weight and the overall expressive force of the bactrocera minax are remarkably reduced, and it is indicated that the fork head transcription factor FOXO of the bactrocera minax plays an important role in the growth and development process of insects. The invention provides a new method for molecular regulation and control of pests, and also provides a theoretical basis for deeply understanding the growth and development mechanism of insects and creating a new biopesticide preparation.

The invention belongs to the technical field of plant genetic engineering, particularly relates to application of a transcription factor CitNAC71 in regulation and control of synthesis of citrus withered water and juice sac secondary walls, and discloses application of the transcription factor CitNAC71 or a biological material for regulating and controlling expression of the transcription factor CitNAC71 in regulation and control of synthesis of citrus withered water and / or secondary walls, and the amino acid sequence of the transcription factor CitNAC71 is shown as SEQ ID NO: 1. The invention discovers that the gene expression level of the coding gene CitNAC71 of the transcription factor CitNAC71 has a significant negative correlation trend with the juice cell withering degree and the cellulose and hemicellulose content, and experiments prove that the CitNAC71 is a conservative fruit hardness regulation factor. The invention lays a theoretical foundation for preventing and controlling the occurrence of dry water before and after the harvesting of the citrus, and provides a potential target for improving the texture of the citrus fruit by a genetic engineering technology.

The invention relates to the technical field of citrus detection in agricultural automation, in particular to a citrus detection method based on a frequency domain aggregation attention mechanism and a multi-scale encoder. According to the method, an innovative detection framework is provided for the problem of small target and occlusion target detection, and the detection framework comprises a frequency aggregation attention network (FAN) and a multi-scale Transform encoder. The frequency aggregation attention network decomposes the feature map through two-dimensional discrete wavelet transform, and enhances the frequency domain features of the small target and the shielding target; the multi-scale Transform encoder is combined with convolution feature pyramid operation, and high-frequency detail information is reserved through a wavelet fusion module. In addition, an IoU is adopted to perceive query selection and optimize a loss function, so that the detection precision is remarkably improved. Through frequency domain feature enhancement and multi-scale feature fusion, high-precision detection of citrus fruits, especially small targets and sheltered targets, is realized, and the method is suitable for fruit grading, yield statistics and other scenes in the field of intelligent agriculture.

The present invention belongs to the technical field of extracting effective pharmaceutical ingredients, and particularly relates to a composition containing citrus polyphenols and citrus powder for burning fat and losing weight, and a preparation method and application thereof. The composition comprises a spray-dried powder prepared from citrus fruit filtrate, Flammulina velutipes fiber, soy protein isolate, aronia berry extract, and black tea extract, and a compound probiotic, wherein the compound probiotic comprises Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium lactis, and Lactobacillus casei. The present invention adopts compound spraying of Flammulina velutipes fiber, soy protein isolate, aronia berry extract, black tea extract, and citrus fruit filtrate to improve the stability of total polyphenols and Vc in citrus fruits. The obtained citrus powder is further mixed with a compound probiotic to prepare a composition with pharmacologically active ingredients, which can effectively burn fat and lose weight and can be applied to weight loss drugs.

The invention relates to the technical field of agricultural automatic intellectualization, in particular to a citrus picking and obstacle detection method and system, and the method comprises the following steps: building a detection image evaluation model, and carrying out the evaluation and analysis of original citrus detection image data, and obtaining citrus target detection image information; determining analysis characteristic parameters of the citrus detection image according to citrus picking and obstacle detection requirements, and further constructing a citrus detection image multi-level analysis system; setting calculation prediction models of different feature analysis layers in the orange detection image multi-level analysis system according to the analysis feature parameters, and analyzing the target detection image information by combining the multi-level analysis system and the calculation prediction models to obtain a multi-level analysis result of the orange detection image; on the basis of a multi-level analysis result and citrus target detection image information, citrus fruit types and obstacle conditions are analyzed, and different citrus picking schemes are formulated. According to the invention, automation of citrus picking and obstacle detection is realized, and agricultural intelligent development is facilitated.

The invention discloses an antioxidant repairing composite composition containing a picea norvegensis leaf extract and a preparation method thereof, and relates to the field of skincare products, the picea norvegensis leaf extract, leontopodium alpinum and citrus fruit extract are used as main materials, and the picea norvegensis leaf extract and leontopodium alpinum can play a role in the whole pathway of Nrf2 and NF-kappa B, so that the antioxidant repairing composite composition has the advantages that the antioxidant repairing composite composition is free of toxic and side effects; and the citrus fruit extract can inhibit the activation of TRPV1 and relieve the discomfort of the sensitive skin, so that the composition not only can improve the skin barrier of the sensitive skin, but also can inhibit the activity of the TRPV1, and the TRPV1 can be activated only by breaking the barrier and damaging the barrier under the two actions, so that the skin barrier can be well repaired. And the vicious circle of barrier injury can be aggravated by the excessive activation of the TRPV1, so that the stabilization and repair of the skin are realized, and the emulsion provided by the invention has a relatively good DPPH clearance rate and relatively good oxidation resistance.

This invention discloses a biomass asphalt hard carbon material, negative electrode, battery, and preparation method from waste peels of the citrus fruit. It belongs to the technical field of biomass hard carbon materials for sodium-ion batteries. The preparation of the asphalt hard carbon includes: using waste peels of the citrus fruit and asphalt as raw materials, activating them separately with sodium dodecyl sulfate and naphthalene, then performing pre-carbonization and pre-oxidation, followed by mixing, acidification, and carbonization treatments to obtain biomass asphalt hard carbon. This invention efficiently recycles and utilizes the widely available and high-density waste peels of the citrus fruit, turning waste into treasure. Simultaneously, it employs a green and simple preparation method to obtain biomass asphalt hard carbon with a large interlayer spacing, small specific surface area, and abundant closed-cell volume, significantly improving carbon recovery.

The invention belongs to the technical field of fermentation, and particularly relates to a method for preparing an enzyme through multiple fermentation of citrus fruits and the enzyme. The invention relates to a method for preparing enzymes through multiple fermentation of citrus fruits. The method comprises the following steps: S1, pretreatment of raw materials; s2, performing multiple fermentation; and S3, performing post-treatment on the enzyme. According to the present invention, through the complex enzyme synergistic pretreatment, the multi-strain compounding three-stage gradient fermentation and the low-temperature membrane concentration technology, the active components such as vitamin C, flavonoid and the like in the prepared enzyme are efficiently released and most of the active components are retained, the cellulose decomposition rate and the viable count of probiotics are significantly increased, the sensory evaluation is high, and the shelf life is long. The invention provides an innovative solution for industrial production of the citrus enzyme.

The invention discloses a citrus growth promoting fertilizer and a fertilization method thereof, and relates to the technical field of chemical fertilizer production, the citrus growth promoting fertilizer comprises 40-60 parts of macroelements, 15-25 parts of medium elements, 8-12 parts of trace elements, 10-18 parts of an organic active agent, and 5-10 parts of a slow release agent. Therefore, firstly, the nano calcium carbonate is used for replacing a traditional calcium source, and a polylactic acid-fucoidan slow-release membrane technology is combined, so that the dissolved state keeping time of calcium ions in the soil is prolonged by 30-50%, the calcium absorption rate is increased to 35% or above from the average industrial 20-30%, and the fruit cracking rate of citrus is effectively reduced; the bacillus subtilis and the seaweed extract in the organic active agent have a synergistic effect, through biological antagonism and induced resistance, the canker incidence rate is reduced to 1 / 4 of that of a control group, meanwhile, the soil microenvironment is improved through humic acid, and the root system health degree is improved by 40%.

The utility model belongs to the technical field of citrus fruit processing, and particularly relates to a citrus peeling device. The device comprises a support, a first cutter and a second cutter are sequentially and fixedly arranged on the support from top to bottom, and the device further comprises a push rod which is vertically arranged, can move in the axial direction and is used for carrying oranges to move. The push rod can be inserted into the citrus to carry the citrus to move; the multiple first cutters are distributed in the circumferential direction and extend from top to bottom, so that when the push rod carries the citrus to move downwards, the first cutters can conduct peel breaking on multiple positions of the citrus in the circumferential direction; the multiple second cutters are distributed in the circumferential direction, the second cutters and the first cutters are distributed in a staggered mode, and each second cutter is in a barb shape with a downward opening, so that the second cutters can hook the peel in the process that the push rod carries the citrus to the position below the second cutters and then moves upwards. The first cutter and the second cutter in the device are both fixedly arranged, the orange can be peeled only by driving the orange to do linear motion through the push rod, the device is simple in structure, and the manufacturing cost and the maintenance cost are reduced.

The invention discloses a nutrition regulating agent for regulating the mature period of citrus fruits and application of the nutrition regulating agent. The nutrition regulating agent is prepared from potassium nitrate, monopotassium phosphate, magnesium sulfate, ferric sodium ethylenediaminetetraacetate, zinc sodium ethylenediaminetetraacetate, boric acid, ammonium molybdate, gibberellin, abscisic acid and diatomite which is supplemented to 100 parts. The invention further provides a preparation method of the nutrition regulating agent and application of the nutrition regulating agent in regulating the mature period of citrus fruits, and the application mode is that when the diameter of the citrus fruits is 40-50 mm, the preparation is diluted by 600-800 times to be sprayed on leaf surfaces, once every 7 days and twice continuously. Through the synergistic effect of mineral nutrient elements and low-concentration hormones, bidirectional precise regulation and control of the citrus mature period are achieved, fruits can be promoted to mature in advance or later for 7-15 days, the sugar-acid ratio and color of the fruits can be remarkably improved, meanwhile, the problems of tree vigor decline and quality decline caused by single hormone treatment are solved, and the method is suitable for large-scale popularization and application. The method has the characteristics of high efficiency, safety and simple operation.

The tearing and peeling device comprises a rack, a positioning base arranged on the rack and used for bearing citrus fruits, and a lifting main frame capable of ascending and descending in the vertical direction, and the lifting main frame is provided with a cutting knife assembly and a tearing clamping assembly. According to the tearing and peeling device for dried orange peel manufacturing, through the transverse traction action of the tearing clamping assembly, peel is pulled outwards to be separated from pulp in advance based on a longitudinal cutting mark to form a peeling opening, and a variable-curvature guide groove of a guide template is used for limiting a peeling plate to generate a profiling movement track attached to the fruit contour; and the peeling plate is accurately inserted into a skin-flesh gap to complete peeling. According to the device, mechanical reduction of manual flaring and stripping along the cambered surface is achieved, the integrity of three petals of the dried orange peel is guaranteed, meanwhile, extrusion damage to internal pulp caused by rigid cutting is avoided, and the technical problem that fruit shape adaptability and pulp protection are difficult to achieve at the same time through mechanical stripping is solved.

The invention discloses a bactrocera dorsalis point mutation gene editing method based on CRISPR-Cas9 and homologous directional repair, and relates to the technical field of gene editing. The method comprises the following steps: designing high-specificity sgRNA for a PAM sequence adjacent region of a target gene; extending the length of the homologous arm to 100bp, and designing a single-stranded DNA (deoxyribonucleic acid) containing a mutation site as a homologous repair template; after a mixture containing the high-specificity sgRNA, the homologous repair template and Cas9 protein is injected into a bactrocera dorsalis fertilized egg in a microinjection mode, incubation and incubation are carried out, and G0-generation imagoes are obtained through culture; hybridizing the G0-generation imago with wild bactrocera dorsalis, and screening to obtain a G1-generation hybrid mutant; and carrying out selfing by using the G1-generation heterozygous mutant, and screening to obtain a G2-generation homozygous mutant. By adopting the method, the mutation efficiency and embryo survival rate of bactrocera dorsalis can be effectively improved.

The invention belongs to the technical field of traditional Chinese medicines, and discloses an immature bitter orange-derived extracellular vesicle-like nanoparticle, which is characterized in that the immature bitter orange-derived extracellular vesicle-like nanoparticle is prepared by the steps of crushing immature bitter orange, filtering, centrifuging and resuspending, the average particle size is 60-120 nm, and the particle size of the immature bitter orange-derived extracellular vesicle-like nanoparticle is 20-30 nm. The extracellular vesicle-like nanoparticles are used for entrapment of at least one of naringin, neohesperidin, naringenin and hesperidin. The extracellular vesicle-like nanoparticles derived from immature bitter oranges are simple in preparation method, have liver targeting and good stability, and can be used for preparing medicines for preventing and / or treating liver injury, depression and breast cancer.

This invention discloses an anti-hair loss hair root mask, its preparation method, and its application, relating to the field of cosmetic technology. The anti-hair loss hair root mask comprises the following components by weight percentage: 4.0%~9.0% anti-hair loss composition; the anti-hair loss composition comprises the following raw materials: plant extracts, enzyme activators, and a reconstitution system; the enzyme activator is selected from at least one of the following: β-glucosidase derived from Aspergillus niger, β-glucosidase derived from almonds, cellulase complex enzyme, malt extract, and citrus fruit fermentation broth; the plant extract includes Platycladus orientalis leaf extract. The anti-hair loss hair root mask, its preparation method, and its application of this invention achieve a significant increase in raw material utilization and anti-hair loss efficacy through targeted enzymatic release of active ingredients; the innovative product form ensures stable enzyme activity and precise efficacy; it also has scalp oil control and soothing effects and is easily mass-produced industrially.

The application discloses a kind of prebiotic oil citrus juice with oil absorption and defecation promoting effect and preparation method and application thereof, comprising the following steps: S1, after oil citrus fruit is cored, it is soaked, and oil citrus pulp is obtained;S2, juice is extracted, and oil citrus juice and oil citrus pomace are obtained;S3, oil citrus juice is debittered;S4, oil citrus pomace is zymolyzed, and oil citrus residue zymolyzate is obtained;S5, debittered oil citrus juice, oil citrus residue zymolyzate is mixed, then composite prebiotic, white kidney bean extract, enoki mushroom powder, deionized water are added, and after being stirred uniformly, citric acid, vitamin C, lactitol are added, and are mixed uniformly, and it is obtained. By debittering treatment to oil citrus juice, zymolysis and composite additive treatment to oil citrus pomace to improve flavor and extract effective components, then the two are compounded with specific proportion composite prebiotic and other auxiliary materials, not only effectively improve the original bitter and astringent flavor of oil citrus juice, improve the taste of product, but also realize the comprehensive and efficient utilization of oil citrus fruit resources.

The application discloses application of a CsERF017 gene or a protein coded by the same in regulation of resistance of navel orange fruits to sour rot, and belongs to the technical field of molecular biology and fruit and vegetable preservation. A nucleotide sequence of the CsERF017 gene is shown in SEQ ID NO. 2, and an amino acid sequence of the protein coded by the same is shown in SEQ ID NO. 3. The application finds through experiments that the CsERF017 gene is up-regulated after infection of a citrus sour rot pathogen Geotrichum candidum, and silencing or knocking out the CsERF017 gene can enhance the resistance of navel orange fruits to sour rot, and the specific performance is that, compared with a control, the transgenic fruits after inoculation of the G. candidum have reduced symptoms of sour rot, and both a disease index and a TA content are reduced. The application provides an important gene bank and new insights for breeding of citrus disease-resistant varieties and development of molecular markers, and is helpful to fundamentally reduce the dependence on chemical synthetic pesticides in postharvest preservation treatment of citrus fruits.

This invention discloses an anti-hair loss composition, its preparation method, and its application, relating to the field of cosmetic technology. The anti-hair loss composition comprises the following raw materials: plant extracts, enzyme activators, and a reconstitution system; the enzyme activator is selected from at least one of the following: β-glucosidase from Aspergillus niger, β-glucosidase from almonds, cellulase complex enzyme, malt extract, and citrus fruit fermentation broth; the plant extract includes Platycladus orientalis leaf extract; the reconstitution system includes a moisturizing stabilizer, a chelating agent, a buffer system, and deionized water. The anti-hair loss composition, its preparation method, and its application of this invention, through targeted enzymatic hydrolysis technology, efficiently convert inactive glycoside precursors in Platycladus orientalis leaf extract into free flavonoids with targeted anti-hair loss activity, significantly improving raw material utilization and active ingredient yield, achieving a technological breakthrough from "passively adding raw materials" to "actively generating active substances."

Activable pectin-containing citrus fiber, characterized in that the activatable pectin-containing citrus fiber has a yield stress II (crossover) in a 2.5 wt% fiber suspension in demineralized water of 0.1 - 1.0 Pa and a dynamic Weissenberg number in a 2.5 wt% fiber suspension in demineralized water of 4.5 - 8.0, wherein the activatable pectin-containing citrus fiber is obtained by a process comprising: a. Providing a raw material containing cell wall material of an edible citrus fruit; b. Digestion of the raw material by incubation of an aqueous suspension of the raw material at an acidic pH value; c. Single- or multi-stage separation of the digested material from step (b) from the aqueous liquid; d. Washing the material separated in step (c) with an aqueous solution, wherein the aqueous solution is water, deionized water or a salt solution with an ionic strength of l < 0.2 mol / l, and separation of coarse or undigested particles; e. Separation of the washed material from step (d) from the aqueous liquid; f. Washing the separated material from step (e) at least twice with an organic solvent, with subsequent separation of the washed material from the organic solvent each time; g. Drying of the material from step (f) comprising drying at normal pressure to obtain the activatable pectin-containing citrus fiber; wherein the activatable pectin-containing citrus fiber obtained has less than 10 wt.% water-soluble pectin and a yield stress l (rotation) in a 2.5 wt.% fiber dispersion in demineralized water of 1.0 to 4.0 Pa.

The invention belongs to the technical field of citrus pest control, and particularly relates to an insecticidal composition for improving the citrus pest control effect. The insecticidal composition for improving the citrus pest control effect is composed of bis (demethoxycurcumin) and a compound I according to the mass ratio of (1-300): (300-1), and the compound I is pyridaben. According to the pesticide composition, the action mechanism of the bisdemethoxycurcumin to target pests is different from that of other components, the bisdemethoxycurcumin and the other components have a synergistic effect on the panonychus citri when being compounded, and compared with a single effective component, the prevention and control effect on the panonychus citri can be improved, the dosage of pesticide can be reduced, and the pesticide cost is reduced. The pesticide residue of the pesticide on the citrus fruits is reduced, the environmental pollution is reduced, and a support can be provided for seeking a novel efficient medicament for preventing and treating the citrus panonychus citri.

The invention relates to the field of detection, in particular to a method for determining the residual quantity of methoprene and methoprene in citrus through pre-column derivatization liquid chromatography-tandem mass spectrometry, and the method comprises the following steps: S1, preparation of a standard sample and a to-be-detected sample: S11, preparation of the standard sample, and S12, preparation of the to-be-detected sample; s2, preparation and derivation of a standard sample solution and a to-be-detected sample solution: S21, extraction, S22, purification and S23, derivation; s3, measuring; s4, establishing a linear equation; and S5, analyzing the sample to be detected. The technical problems that in the prior art, the ionization efficiency of methoprene and methoprene in the electrospray process is low due to the non-polar property of methoprene and methoprene, and electrospray ionization tandem mass spectrometry detection of methoprene and methoprene lacks the sensitivity required by trace analysis can be solved. The detection sensitivity of methoprene and methoprene in citrus fruits can be obviously improved.