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Bacillus amyloliquefaciens subsp. plantarum ZFH-3 and application thereof

A technology of Bacillus amyloliquefaciens and ZFH-3, applied in the direction of application, bacteria, microorganisms, etc., can solve the problems of unavailable Bacillus amyloliquefaciens products, etc., achieve food safety reduction, strong growth adaptability, and high industrialization feasibility Effect

Active Publication Date: 2015-06-10
珠海真绿色技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few reports on the use of Bacillus amyloliquefaciens in the biological control of postharvest diseases, and there is no marketed Bacillus amyloliquefaciens products for the biological control of postharvest diseases in fruits and vegetables. Therefore, the development of Bacillus amyloliquefaciens for postharvest disease control in fruits Preservation of biological products is of great significance

Method used

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  • Bacillus amyloliquefaciens subsp. plantarum ZFH-3 and application thereof
  • Bacillus amyloliquefaciens subsp. plantarum ZFH-3 and application thereof
  • Bacillus amyloliquefaciens subsp. plantarum ZFH-3 and application thereof

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Experimental program
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Effect test

Embodiment Construction

[0043] 1. Preparation and identification of ZFH-3

[0044] Bacillus amyloliquefaciens subsp. plantarum ZFH-3

[0045] 1. Sample collection

[0046] Collect soil samples from old forests in Datian County, Sanming City, Fujian Province, collect edible fungi of the Basidiomycetes Agaricaceae Russulaceae, and dry them naturally; cut off the roots of edible fungi, grind them into powder, and mix them evenly to obtain root samples of edible fungi; soil samples Grind into powder, mix evenly, and obtain soil samples;

[0047] 2. Isolation of strains and screening and purification of resistant bacteria

[0048] (1) Sample dilution: From the 2 samples (fungus root sample and soil sample) that were mixed uniformly, each weighed 5g and dissolved it in a conical flask filled with 45ml sterile water, and vibrated with a 150rp oscillator to form a uniform 10 -1 diluent, and then use a 1ml pipette gun to draw 1ml of the diluent into a test tube filled with 9ml sterile water, and shake it wel...

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Abstract

The invention discloses a Bacillus amyloliquefaciens subsp. plantarum ZFH-3 and application thereof. The classification designation of the strain is Bacillus amyloliquefaciens subsp. plantarum, and the collection number is CGMCC No.9884. The strain has obvious inhibiting actions on multiple crop pathogens, especially can effectively control postharvest diseases of fruits, is well used for biological control of contact mold of citrus fruit, olive green mould of ditrus fruit, citrus phomopsis stem end rot, sour rot of citrus, anthracnose of citrus, anthracnose of banana, spike rot of banana, gray mold of strawberry, soft rot of strawberry and other pathogen fungi, obviously lowers the possibility of postharvest diseases of fruits, prolongs the storage life and shelf life, and reduces the consumption of chemical reagents. The strain has the advantages of simple culture conditions and high reproduction speed, can easily implement industrial production, popularization and application, and has favorable development and application prospects.

Description

technical field [0001] The invention belongs to the fields of fruit preservation and post-harvest disease prevention, and relates to Bacillus amyloliquefaciens plant subspecies ZFH-3 and its application, especially in the prevention and fresh-keeping of main diseases of fruits such as citrus, banana and strawberry after harvest. Background technique [0002] Postharvest disease of fruit is the main factor affecting the healthy development of fruit industry, and it is also the focus of research on postharvest storage and transportation of fruit. Postharvest diseases of citrus mainly include penicillium, green mold, stalk rot, sour rot and anthracnose. Post-harvest diseases of bananas are mainly shaft rot (crown rot) and anthracnose. Post-harvest strawberries are not storable and easy to rot, mainly gray mold and soft rot. Most of the postharvest diseases of fruits are infectious diseases, most of which invade from the navel, pedicle and wound, and can be cross-infected throu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/07
CPCA23B7/155C12N1/20C12N1/205C12R2001/07
Inventor 吴日章
Owner 珠海真绿色技术有限公司
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