1529 results about "Biological pest control" patented technology

The invention relates to a strain of lys-starch bacillus as well its application. The bacterium is separated from the soil on eggplant fields, anning district, Lanzhou, Gansu. The bacterium is identified to be Bacillus amyloliquefaciens. It's preserved in the Chinese typical culture preserving center (CCTCC) with a preserving name of Bacillus amyloliquefaciens I7 and preserving code of M206027. The broth of M206027 and M206027strain, non-cell culture fluid of M206027 strain's broth, and the monomer extracted from the M206027strain, M206027 broth or its non-cell culture fluid can be used in the biological control of peach anthracnose bacterium, pear nigrities artis bacterium, apple brown rot bacterium, Fusarium acuminatum, Fusarium oxysporum, Fusarium semitectum, chilli black rot bacterium, cucumber blight bacterium, tomato early blight bacterium, eggplant early blight bacterium, dry thread Pyrenomycetes, wheat foot rot bacterium, potato thread Pyrenomycetes, potato early blight bacterium, potato late blight bacterium and watermelon downy mildew bacterium. It's easy to get the material of the invention and easy to operate; besides, it can be widely used in full scale production.

The invention discloses Bacillus subtilis, which is named Bacillus subtilis. The Bacillus subtilis was collected in China General Microbiological Culture Collection Center on January 15th, 2009, with a collection number of CGMCC No.2843. The Bacillus subtilis strain disclosed by the invention is screened from tobacco leaf habitats. Experiments prove that the fermentation liquor of the strain has a strong inhibition effect on fungal pathogens such as Alternaria alternate and pepper anthracnose and demonstrates high prevention effect on Alternaria alternate, tobacco black shank, pepper anthracnose, tomato wilt, phytophthora melonis Katsura, verticillium dahlia and the like and has high growth promoting effects on tobacco, pepper, cucumber, tomato and eggplant. Therefore, the Bacillus subtilis disclosed by the invention can be used for preparing biological bactericides for preventing and controlling fungi in tobacco and vegetables and can be used for preparing growth promoting agents for promoting the growth of tobacco or vegetables.

The invention discloses a strain of Streptomyces and application thereof. The biocontrol Streptomyces is blastmycin Streptomyces and its strain number is JZB130180, which is registered in the general microbiological center of China Committee for Culture Collection of Microorganisms with CGMCC No. 13270. The strain had significant antagonistic effect on 14 kinds of plant pathogenic fungi and 6 kinds of plant pathogenic bacteria in agricultural production, and has wide antimicrobial spectrum. The strain produces chitinase, cellulase, protease and selenium, and has the basic characteristics of biocontrol bacteria. The fermentation broth of this strain has strong control effect on tomato gray mold and peach brown rot, and has significant promotion action on the tomato plants, which indicated that the strain had a broad application prospect in the field of biological control of plant diseases.

The invention provides a systematic agricultural production system, in which materials and energy are designed to be circular. The system comprises a solid breeding area, a solid planting area and a biological pest control area. The whole planting and breeding production adopts a solid industrial modular production mode; energy needed for supplementing the temperature and solid light source of the planting area and the breeding area is provided by a biomass energy generation system and an energy supplementary system consisting of solar and wind energy. Leftover bits of the planting area are transformed into feed used in the breeding area after being treated and leftover bits of the breeding area are produced into biological mediums which are supplied to the planting area after being go through the microbial action. Carbon dioxide and oxygen generated between the breeding area and the planting area are recycled mutually and the temperature and the humidity conditions of the breeding area and the planting area are transformed mutually. The system of the invention can carry out effective combination and the reforger of an industrial chain for traditional agriculture, marine agriculture, microorganism and lower living things.

The invention discloses a method for propagating Phytoseiulus Persimilis, which comprises the steps: planting and cultivation of host plant, inoculation and feeding of Tetranychus cinnabarinus, inoculation and feeding of Phytoseiulus Persimilis as well as collection, package and refrigeration of Phytoseiulus Persimilis. The method takes mass propagation of Phytoseiulus Persimilis as the purpose, provides technical support for feeding Phytoseiulus Persimilis on scale, and provides special predacity natural enemy for Tetranychus pest mites on greenhouse crops for biological control. The invention is a production method in which living hosts (firstly host plant and host Tetranychidae are cultivated) are adopted for feeding Phytoseiulus Persimilis, and makes a series of breakthroughs on key techniques for artificial mass propagation of Phytoseiulus Persimilis. The method of the invention can lead the production cost of Phytoseiulus Persimilis to be stabilized, the production period to be 25 days, and the production scale to reach 10,000/m<2>.

The invention provides a method for propagating Oriusminutus artificially, which comprises the following steps: cultivation of spawning substrate, feeding of host; preparation of instruments of production, preparation of artificial feedstuff and preparation of seeds are carried out; under the conditions of 25 DEG C, 75% of PH and 16L:8D of illumination, kidney bean buds are taken as the spawning substrate, and Tetranychus cinnabarinus or the artificial feedstuff is used for feeding; firstly, the kidney bean buds on which the Oriusminutus spawn is produced are placed in a large tray, and kidney bean seedlings with proper Tetranychus cinnabarinus are placed in the large tray by interpenetration, according to the age period of Oriusminutus, the number of the proper Tetranychus cinnabarinus is confirmed; then the Oriusminutus with the tray are placed in an insect breeding cage for feeding the Oriusminutus. Spawn needs 12-16 days to grow to be imago, and the production period is about 30 days. The method is applicable to feeding with large amount and being promoted, thus laying the solid foundation for producing Oriusminutus with large amount and providing great convenience to biological control.

The invention relates to heavy metal resistance plant growth-promoting bacteria and a preparation applying method thereof, which belongs to the bioremediation field of heavy metal polluted environment. A bacteria strain D54 with a preservation number of CGMCC No. 3223 belongs to the Burkholderia sp. and has higher resistance to a plurality of heavy metals, wherein the resistances to Pb <2+>, Cd <2+>, Cu <2+>, Zn <2+> respectively reach 800 mg/L, 1500 mg/L, 150 mg/L and 2500 mg/L. In addition, the bacteria strain D54 has the plant growth-promoting functions of producing plant growth hormone (IAA), producing 1-amino-1-carboxyl cyclopropane (ACC) deaminase, secreting siderophore, dissolving inorganic phosphate, fixing nitrogen and the like, has the biological prevention functions of antagonizing plant pathogenic bacteria inbreak and the like, and can obviously improve the biomass of the plants applied the invention and improve the resistance to diseases and stresses. The number of the effective viable bacteria in liquid preparation reaches 1-2 billion/ml and the number of the effective viable bacteria in solid preparation reaches 1 billion/g. Soaking seeds for 1-2 hours in the liquidpreparation which is diluted 100 times and irrigating the diluted liquid preparation 1-2 times (10ml/kg) after 2-3 weeks of the sprouting of the seeds can effectively improve plant viable bacteria infection probability.

The invention discloses bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding diseases. The collection number of the Bacillus amyloliquefaciens H47 is CCTCC NO: M2013283. A biological agent which resists the peach bleeding diseases is prepared by carrying out liquid fermentation on the strain can be applied to peach trees; the liquid fermentation process comprises the following steps of: slant strain activation, seed culture and liquid deep fermentation culture. The strain disclosed by the invention and thallus-free spore-free fermentation liquor can both generate a higher antagonistic effect on the pathogen botryosphaeria of the peach bleeding diseases; the biological agent disclosed by the invention achieves an outstanding preventing and controlling effect on peach tree bleeding diseases; compared with the traditional chemical agent, the biological agent disclosed by the invention has the advantages of greenness, safety, environment friendliness, and the like and has wide application prospect on the aspect of biological prevention and control of the bleeding diseases.

The microbial fertilizer is mixture of fermented liquids separately with photosynthetic bacteria, bacillus, actinomycete and lactic acid bacteria. It is prepared through inoculating photosynthetic bacteria, bacillus, actinomycete and lactic acid bacteria separately to the first stage culture medium, and culturing to obtain the first stage bacterial seeds; inoculating the first stage bacterial seeds separately to second stage culture medium, and culturing to obtain the second stage bacterial seeds; and inoculating the second stage bacterial seeds separately to the fermenting culture medium, and culturing to obtain fermented bacterial liquids; and mixing the fermented bacterial liquids in certain proportion to obtain the microbial fertilizer. The present invention also relates to the application of the microbial fertilizer in controlling diseases of tobacco and trees and the usage.

The invention discloses a method for producing fungi in a strain of plant and the application. Fungi which is produced in plants is Acremonium strictum HND5 CGMCC NO.2192. The bacterial strain and metabolite thereof can inhibit pathogen fungi activity in broad spectrum, can produce activity volatility natural gas chemical compound which can inhibit the activity of pathogenic fungi, the bacterial strain can parasitize banana root-knot nematode, metabolite can poison banana root-knot nematode, the bacterial strain can be widely used in the aspects of biologically preventing and curing plant diseases and pests, arm-shaped grass production, and in the aspects of fungi modified and breeding material.

The invention relates to an overwintering preserving breeding method for an aphidius gifuensis and belongs to the technical field of biological control. The overwintering preserving breeding method for the aphidius gifuensis solves the problem that in the prior art, overwintering preservation is performed on the aphidius gifuensis difficultly, and the heavy demand for the aphidius gifuensis in a tobacco planting season in a coming year is difficult to meet. The overwintering preserving breeding method comprises a first step of greenhouse aphidius gifuensis selection which comprises cultivation of host plants, collecting of an aphidius gifuensis source, aphidius gifuensis inoculation in a greenhouse, and purification and rejuvenation; a second step of winter greenhouse aphidius gifuensis preservation which comprises cultivation of aphidius gifuensis preserving plants, aphidius gifuensis inoculation in the greenhouse, and purification and rejuvenation; and a third step of expanding propagation in the greenhouse. The aphidius gifuensis is successfully accumulated and preserved during the time from finish of massive reproduction of the aphidius gifuensis in August to April in a coming year and before massive reproduction of the aphidius gifuensis, and the aphidius gifuensises with enough quantity and good quality are provided for massive reproduction of the aphidius gifuensis in a next year.

Belonging to the field of biological control of pests, the invention discloses a Bacillus thuringiensis strain that has a broad spectrum and is highly effective on lepidoptera pests, preparation of its wettable powder and application. The Bacillus thuringiensis YX-1 strain involved in the invention is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 5256. Containing a plurality of insecticidal genes, the strain has a highly effective lethal effect on Cydia molesta, cotton bollworm, fall webworms, Adoxophyes orana Fisher von Roslerstamm, Phalera flavescens Bremer et Grey, carpocapsa pomonella, prodenia litura, plutella xylostella, beet armyworm and other lepidoptera pests. The invention also discloses a method for preparation of wettable powder through the YX-1 strain. The Bacillus thuringiensis YX-1 strain and its wettable powder provided in the invention are mainly used for prevention and treatment of lepidoptera pests on various fruit trees and vegetables, also can be used for prevention and treatment of lepidoptera pests on field crops and garden plants, and have the characteristics of broad spectrum, high efficiency, safety to people, livestock as well as animals and plants, and no environmental pollution, etc.

The invention provides a biocontrol bacterial strain for preventing and treating plant diseases and its bacterial agent, belonging to the field of biological control. The strains used are Gram-negative bacteria, identified as Lysobacter enzymogenes, and the strain code is OH11. Strain OH11 has no flagella but has slippage, can produce various extracellular hydrolytic enzymes including chitinase, β-1,3-glucanase and protease, and can effectively inhibit the growth of fungi and bacteria. The antibacterial zone diameters of strain OH11 against Sclerotinia sclerotiorum and Phytophthora capsici were both greater than 22.0 mm; the antagonism against potato ring rot was stronger, and the diameter of the inhibition zone reached 50 mm. The OH11 strain was inoculated into the seed tank, cultivated to the logarithmic growth phase, and the seed liquid was connected to the production tank for cultivation. The medium used in the production tank was the same as that of the seed tank. The liquid fermentation adopts aerobic submerged fermentation and fed-batch process, the dissolved oxygen is 15%-20%, the fermentation temperature is 30°C, the fermentation time is 72h, and the initial pH value is 7.5. After the fermentation is completed, the culture solution is taken out of the tank and directly packed into liquid dosage forms with plastic packaging barrels or packaging bottles, or subpackaged into solid dosage forms with peat adsorption packaging bags. The biocontrol strain OH11 can effectively control plant pathogenic fungi, bacteria, nematodes and other diseases, and the overall control effect is 50%-70%. In the greenhouse pot experiment, the control effects of OH11 on pepper blight and tomato bacterial wilt reached 83.6% and 86.4%, respectively. Strain OH11 has the characteristics of broad antibacterial spectrum, high activity, and environmental safety. In today's serious pesticide pollution, zymolysobacterium and its bacterial agent will be a good substitute.

The present invention is directed to isolated Bacillus subtilis, Pseudomonas putida, and Sporobolomyces roseus which are useful as a biocontrol agent. These organisms are useful in a method of imparting to plants protection against plant pathogens by applying them to plants, plant seeds, or soil surrounding plants under conditions effective to impart disease protection to the plants or plants produced from the plant seeds. The biocontrol agents are also useful in a method of enhancing plant growth which involves applying them to plants, plants seeds, or soil surrounding plants under conditions effective to enhance growth in the plants or plants produced from the plant seeds.

The invention discloses bacillus amyloliquefaciens JK6 and biological fertilizer and application. The serial number of strain preservation is CGMCC No. 10658 and is stored in the China general microbiological culture collection center on March 23, 2015. The bacillus amyloliquefaciens JK6 is mainly used for biological prevention and control of pseudomonas solanacearum and is provided with an iron-production carrier, a biological membrane, protease, catalase, cellulose and IAA characteristics, multiple fungal pathogens of banana wilt pathogen, litchi anthrax germs, litchi frost phytophthora pathogens, rice blast fungus, cucumber wilt pathogen and the like, and growth of lettuce plants is obviously promoted; the K6 strain can be successfully planted, and disease preventing functional genes of yndj, srfAB, fend and ituC are expressed in soil; the strain is high in reproduction speed, simple in production technology, high in adverse resistance, easy to store and favorable for industrialized production, the biological fertilizer is prepared with the strain, soil-born diseases can be prevented and controlled, growth of the plants can be promoted, and application prospect is broad.

The present invention provides a compound for controlling wood parasitism pest as well as a method for preventing wood parasitism pest. That is the present invention provides a compound containing O-ethyl-S-propyl[(2E)-2-(cyano-imino)-3-ethyl-1-imidazolealkyl]thionophosphonates as active component, a method for controlling the wood parasitism pest using said compound, as well as a method for protecting wood using said compound.

The invention relates to a method for controlling pests. The method includes the step that the pests, namely sesamia inferens, are in contact with CrylA protein. The sesamia inferens are controlled in the mode that the CrylA protein capable of killing the sesamia inferens is provided in plants. Compared with an agricultural prevention method, a chemical prevention method and a biological prevention method in the prior art, the method can protect the whole plants during the whole growth period so as to prevent attack of the sesamia inferens, and is free of pollution, free of residual pesticide, stable and complete in effect, simple, convenient to carry out and economical.

The invention discloses a Bacillus siamensis strain for preventing and treating pear ring rot and soft rot and application thereof. The Bacillus siamensis strain is separated from peels of Laiyang pears with sterilized surfaces, the strain has broad-spectrum resistance for various types of phytopathogens and has a good effect on preventing and treating the pear fruit rot caused by pear ring rot and soft rot. The strain comes from pear fruits and is safe to pear fruits; the strain is produced on a large scale, is low in raw material cost, convenient to operate and stable in prevention and treatment effect, and can effectively solve the problems of agricultural residues and environment pollutions caused by pear fruit disease and insect pesticide prevention and treatment. The strain has huge application potential on the aspects of biological prevention and treatment of after-harvest diseases and insects of pears, and the excellent basic strain is provided for research and development of microbial pesticides.

The invention discloses application of streptomyces griseoflavus in biological prevention and control of plant diseases, particularly streptomyces griseoflavus NMG6-3-9 CGMCC No. 3441 in biological prevention and control of plant diseases. The provided strains have good antagonism effect on pathogenic bacteria of alfalfa root rot, can be used for the biological prevention and control of alfalfa root rot and other plant diseases, and have wide application potential in the field of biological prevention and control of plant diseases. The invention lays a foundation for the biological prevention and control of alfalfa root rot and further provides a scientific basis for the development and the application of a biological prevention actinomycete preparation.

The invention provides efficient phosphate solubilizing growth-promoting bacteria and a microbial agent prepared from the same. The active ingredients of the microbial agent are pantoea ananatis P21 and hirsutella minnesotensis. According to the invention, the widely-applicable efficient phosphate solubilizing bacteria pantoea ananatis P21 and fungi for efficiently preventing nematodiasis are organically combined; and by utilizing the composite agent provided by the invention, the soil phosphorus is efficiently used, the utilization rate of phosphate fertilizer is improved, the function of microorganism in preventing nematodiasis is sufficiently realized, and an effect of increasing yield by solubilizing phosphate and preventing diseases is realized, thereby having great significance to sustainably increasing grain yield of the country, saving phosphate fertilizer resource, reducing nematodiasis and protecting the ecological environment of farmland.

The invention discloses a plant pathogenic fungus antagonistic bacterium strain and application thereof in preventing and treating plant diseases. The antagonistic bacterium is Bacillus subtilis ZSH-1, and the collection number is CGMCC NO.9025. The Bacillus subtilis ZSH-1 is separated from poplar root soil, and can prevent and treat plant diseases caused by poplar anthracnose bacterium, poplar rot bacterium, willow rot bacterium, apple rot bacterium, birchleaf pear rot bacterium, Fusarium oxysporum and date corky core bacterium. The antibacterial volatile matters generated by the antagonistic bacterium can also prevent and treat plant diseases caused by poplar anthracnose bacterium, poplar rot bacterium, willow rot bacterium, apple rot bacterium, birchleaf pear rot bacterium, Fusarium oxysporum and date corky core bacterium. The Bacillus subtilis ZSH-1 is a biological control potential strain with high prevention effect, wide preventing and treating range and high environmental safety, and has favorable development and application prospects.

The invention provides a rice-duck culture method. The method comprises the following steps of 1, working duck selection, 2, duck releasing condition and time selection, 3, duck releasing site and area selection, 4, natural enemy protective wall building, 5, rice field parcel selection and building, 6, rice variety selection, 7, transplanting, 8, biological control of insect pests and weeds, 9, water management, 10, fertilization and 11, duck catching and rice harvesting. Compared with the prior art, the appropriate working ducks are selected, the ducks are released in good time, and a foreign protective wall is built based on the specific releasing density; an appropriate field parcel is selected, an appropriate transplanting method is built, the appropriate water management and fertilization technology are built, finally, the duck and rice yield and quality are improved, and the ecological benefits of the rice field are remarkably improved.