28 results about "Biological pest control" patented technology

The invention discloses a sex attractant for bactrocera dorsalis and application of the sex attractant. The invention provides a sex attractant for bactrocera dorsalis. The sex attractant for bactrocera dorsalis comprises ethyl laurate, ethyl myristate, cis-9-hexadecenoic acid ethyl ester and ethyl palmitate. The sex attractant provided by the invention can effectively reduce the population density of male adult bactrocera dorsalis in the field, reduce successful mating of female adult bactrocera dorsalis, and reduce the reproduction rate of bactrocera dorsalis. The sex attractant developed by the invention is a novel bactrocera dorsalis attractant, can enrich compound resources for biological prevention and control of bactrocera pests and promote the development of a pest comprehensive prevention and control strategy towards the directions of high efficiency, economy, greenness and the like, and has very high application value.

The invention relates to application of a yeast containing Arabic gum component in feeding of Olin Bayesian mites and predatory mites and a breaking method of the Olin Bayesian mites and the predatory mites. The method is characterized in that high-efficiency feeding of the Olin mites can be realized by utilizing yeast containing Arabic gum, but the fed Olin mites are not beneficial to the high-efficiency production of the predatory mites, so that the high-efficiency production of the predatory mites cannot be realized; yeast just containing Arabic gum plays a role in inhibiting and limiting production of the predatory mites, and even leads to absolutely species of the predatory mites is applied to feeding of the Olin Bayesian mites and the predatory mites. The invention not only provides a method capable of limiting and removing the predatory mites without influencing mass feeding of the Olin Bayesian mites, but also provides a solution for solving the problems caused by the fact that the Olin Bayesian mites fed by the yeast containing the Arabic gum component are not beneficial to the production of the predatory mites. The invention mainly aims to provide a brand new thought and scheme for biological control of pests and pesticide reduction or avoid unnecessary loss of related enterprises.

The utility model relates to the technical field of biological control, in particular to a pest biological control throwing device suitable for grassland, which is characterized in that a reciprocating structure is arranged at the top of a base, power structures are uniformly arranged on the periphery of the bottom of the base, and the base is connected with a pushing plate through the reciprocating structure; and the pushing plate is fixedly connected with pushing pipes which are evenly distributed, soil blocking structures are arranged in the pushing pipes, the reciprocating structure comprises a first supporting column fixedly connected with the base, and the top of the first supporting column is rotationally connected with a driven gear. When the device is used, a microbial preparation is placed in the pesticide containing box, a water pump in the pesticide containing box is used for conveying the pesticide to the pushing pipe from the hose through the pushing plate, the reciprocating structure is started to drive the pushing plate, the pushing plate drives the pushing pipe to prick into soil, and pesticide release is completed through the soil blocking structure in the pushing pipe. The biological control effect is achieved.

The invention relates to the technical field of biological prevention and control of pests, in particular to a breeding method of natural enemy insects, and particularly relates to a prevention and control method of a bee colony juvenile beauty disease, which comprises the following steps: filling a mixed solution of a cinnamon leaf aqueous solution and an oxytetracycline aqueous solution into a sprayer, and spraying to a sick bee colony until the body surfaces of larvae are wet without dripping water. According to the scheme, the mixed solution of the cinnamon leaves and the oxytetracycline has a good prevention and treatment effect on the meloidosis of the wasp colony, the loss caused by diseases to the wasp colony can be greatly retrieved, the success rate of the primary wasp colony can be greatly increased, and the mixed solution has small side effects on wasps and is low in cost.

The present invention in a first aspect relates to a system for releasing beneficial arthropods. Further aspects relate to methods for biological pest control and the use of the system in pest control. The present invention further relates to a system for storing beneficial arthropods and a method for manufacturing such a system. All aspects of the present invention are useful in biological pest control, particularly in crop protection.

The invention relates to the technical field of biological prevention and control of pests, in particular to an shRNA (short hairpin Ribonucleic Acid) nanocomposite, preparation thereof and application of the shRNA nanocomposite to prevention and control of spodoptera frugiperda. The shRNA nano-composite provided by the invention comprises an shRNA related element and a cell penetrating disulfide polymer, the shRNA related element is selected from shRNA, a recombinant vector containing the shRNA and a recombinant cell containing the recombinant vector containing the shRNA; wherein the shRNA (short hairpin ribonucleic acid) is selected from one or two of shCHSB (short hairpin CHSB) or shCOPIbeta (short hairpin COPIbeta); wherein the cell-penetrating disulfide polymer is used for protecting the shRNA and delivering the shRNA at the same time, so that the effective prevention and control of the spodoptera frugiperda are successfully realized. The shRNA nanocomposite provided by the invention realizes relatively high silencing efficiency aiming at the CHSB gene and the COPIbeta gene of the spodoptera frugiperda, and generates a remarkable inhibition effect in the subsequent growth and development process of the spodoptera frugiperda.

This invention relates to the field of artificial breeding technology for natural enemy insects, and in particular to a method for the artificial breeding of the assassin bug *Triplophysa pulcherrima*. The invention employs steps including seed source collection and screening, rearing environment control, feed supply and management, mating and reproduction management, oviposition and egg mass collection, nymph hatching and age-differentiated rearing, overwintering management, and cannibalism control. By providing specialized rearing management devices, temperature and humidity conditions, standardized feed feeding, age-differentiated rearing density control, and moisture-controlled egg mass hatching, it solves the problems of high mortality rates among young nymphs, unstable mating success rates, low egg mass hatching rates, and the lack of a year-round breeding system in existing technologies. The method of this invention is simple to operate, has a high survival rate (nymphal survival rate exceeding 85%), an egg mass hatching rate exceeding 90%, and can achieve 4-5 generations per year, enabling large-scale, year-round production of *Triplophysa pulcherrima*, providing high-quality natural enemy resources for the biological control of agricultural and forestry pests.

The application discloses a pest trapping device and method based on biological control, which comprises a mobile vehicle body and a pest trapping assembly arranged on the mobile vehicle body; the pest trapping assembly comprises two groups of frame body assemblies, a transmission ring arranged between the two groups of frame body assemblies, and two winding rollers rotatably arranged in the transmission ring; the transmission ring is in a cylindrical shape and is provided with a through groove at the top; the two winding rollers are both provided with rewettable adhesive tapes; the rewettable adhesive tapes are attached to the outer walls of two semicircular sections formed by the peripheral wall of the transmission ring to form trapping adhesive areas; and the rewettable adhesive tapes are continuously unwound, the design of the trapping channel groove mechanism and the spraying assembly for spraying liquid is used, the flowability and replaceability of the adhesive areas are realized, and the adverse effects caused by the continuous replacement of the adhesive tapes are avoided.

The invention belongs to the technical field of pest biological control, and particularly relates to a full-open type monochamus alternatus head mite releaser and a releasing method.The full-open type monochamus alternatus head mite releaser comprises a top cover, a cross-shaped baffle, a monochamus alternatus collecting funnel and a monochamus alternatus lure, the upper end and the lower end of the cross-shaped baffle are fixedly connected with the top cover and the monochamus alternatus collecting funnel respectively, and the upper end and the lower end of the cross-shaped baffle are fixedly connected with the top cover and the monochamus alternatus lure respectively; a hollow part is arranged in the middle of the cross baffle plate, a monochamus alternatus lure is arranged at the hollow part, and a monochamus alternatus head mite attractant is injected into the monochamus alternatus lure. According to the invention, large-scale accurate release of the small head mites of the monochamus alternatus can be realized, the control effect of the monochamus alternatus is improved, and the method is suitable for further popularization and application.

The invention relates to the technical field of biological control, in particular to a method for screening high-toxicity locust pathogenic strains based on carbon and nitrogen metabolism and application of the high-toxicity locust pathogenic strains based on carbon and nitrogen metabolism. And a strain with high toxicity to migratory locust is screened out. ITS (ribosome DNA spacer) sequences of fungi are amplified through ITS1 and ITS4 primers, sequence comparison and phylogenetic analysis are carried out through MEGA 7.0 software, and it is ensured that the strain is metarhizium ledebusii; the method can be used for systematically screening high-toxicity metarhizium ledebusii strains, can be used for biological control of pests, and provides a scientific basis for development of efficient and sustainable pest control strategies.

The utility model provides a biological prevention and control device for stored insect pests, which belongs to the technical field of biological prevention and control and comprises a bin shell, a fixing groove formed in the side wall of the bin shell, an insect repelling block clamped in the center of the fixing groove and a dismounting component arranged on the inner wall of the insect repelling block. The multiple fixing grooves are formed, and the multiple insect repelling blocks correspond to the multiple fixing grooves. According to the utility model, through the matched layout of the plurality of detachable insect expelling blocks and the fixing grooves, the device can flexibly adjust the prevention and control point positions according to the storage space; the innovative dismounting assembly adopts a spring clamping block mechanism, so that one-key type quick replacement of the insect expelling block is realized, and the maintenance difficulty is greatly reduced; double locking of the elastic block and the clamping groove ensures installation stability, and looseness caused by long-term use is avoided; the operation component is linked with the pull rod through the bearing seat, so that the replacement process is labor-saving and convenient; the whole structure is good in sealing performance, volatilization of insect repelling components is effectively delayed, and the technical problems that a traditional device is fixed in layout, difficult to maintain, poor in sealing performance and the like are solved.

The invention belongs to but is not limited to the technical field of pest biological control, and discloses a method for relieving reproductive decline of harmonia axyridis, which is suitable for indoor long-term breeding populations incapable of avoiding inbreeding, in particular to rare homozygous mutants needing long-term conservation. The method specifically comprises the following steps: feeding offspring new eclosion adults which show reproductive decline after being fed with aphids as food with artificial feed prepared by mixing pork liver homogenate, rape pollen, cane sugar, yeast powder, honey and the like for 3-4 days, and then feeding the adults with the aphids; in the feeding process after the ladybirds start to lay eggs, 7-day aphids and 1-day artificial feed are adopted for feeding circularly, and high reproductive power is kept. In addition, when severely reproductive decline occurs to adults fed with the aphids in daily life, the adults are fed with artificial feed for 3 days in time, then aphid feeding is recovered, and the egg laying amount and the egg hatching rate can be remarkably increased within a short time. According to the method, the reproductive decline of the harmonia axyridis bred indoors for a long time is effectively relieved through short-term intermittent artificial feed feeding.

The application provides a Chinese trichopoda japonica attractant and a preparation method and application thereof, and belongs to the technical field of biological pest control. The active ingredient of the Chinese trichopoda japonica attractant is composed of volatile substances of potentilla chinensis, and the volatile substances include (Z)-3-hexen-1-ol 10-15 parts, gamma-terpinene 10-20 parts, trans-beta-myrcene 60-70 parts and nerolidol 0.5-1 part in volume fraction. The Chinese trichopoda japonica attractant as the effective component is combined with a diluent to further obtain an attractant preparation. The volatile substances in the potentilla chinensis are used to perform olfactory behavior response on the Chinese trichopoda japonica and are applied, the obtained attractant or attractant preparation has obvious attracting effect, is harmless to other natural enemies and is environment-friendly, and provides a new reference means for biological control.

The invention relates to the technical field of agriculture, belongs to a pest biological prevention and control technology, and relates to a method for prevention and control of sorghum aphids and cultivation of aphis gossypii. The prevention and control method for the sorghum aphids comprises the steps that aphis gossypii gossypii is released in a field with the sorghum aphids, the aphis gossypii gossypii is used for preventing and controlling the sorghum aphids, and the ratio of the released aphis gossypii gossypii to the field sorghum aphids is larger than or equal to 1: 50. According to the method, the aphis gossypii is adopted to prevent and control the sorghum aphids, so that the situation that pesticides are used for removing the aphids on the sorghum is avoided, the sorghum pest control cost is saved, pesticide residues of the sorghum are reduced or eradicated, and the safety of the sorghum serving as a food source is improved.

The present invention relates to a novel bacterium Pseudomonas sp. 14og as deposited into the NRRL Patent Culture Collection on 3 Dec. 2020 and designated NRRL accession no. B-67999. The present invention also relates to insecticidally active substances obtained or derived from Pseudomonas sp. 14og. The insecticidal substances find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, Hemipteran, Tetranychidae, fungal, nematode and mite pest populations and for producing compositions with insecticidal activity. The present invention relates to a novel bacterium and use for biocontrol of insect pests. More particularly, the invention relates to a newly discovered species of Pseudomonas bacterium that exhibits insecticidal activity. The invention also relates to proteins and / or peptides and / or metabolites obtained from the strain that possess insecticidal activity and to insecticidal compositions comprising cultures of the novel strain and / or supernatants, filtrates, and extracts obtained from the strain, and use thereof to control insect pests. The present invention relates to a novel bacterium and uses thereof. In particular, the present invention relates to applications concerned with the biopesticidal activity of the novel bacterium and of its mutant or variant strains. The present invention also concerns proteinaceous substances produced by this novel bacterium and of its mutant or variant strains obtained or derived from the aforesaid bacteria.

The present invention according to a first aspect relates to a system for releasing beneficial arthropods. Further aspects relate to a method for biological pest control and the use of the system in pest control. The invention furthermore relates to a system for storing beneficial arthropods and a method for producing such a system. All aspects of the invention have utility in biological pest control, in particular in crop protection.

The orchard pest trapping and natural enemy conservation device comprises a pest collecting groove, a supporting piece, a flashing board and a lure, the pest collecting groove is used for being filled with trapping and killing liquid, and the lure is used for being filled with insect sex attractants. When the orchard pest trapping and natural enemy conservation device is used, the pest collecting groove and the flashing board can be arranged on a trunk in a sleeving mode, then the trapping core is filled with insect sex attractants, the pest collecting groove is filled with trapping and killing liquid, and amphoteric adults of various pests can be trapped and killed at the same time through the insect sex attractants; the trapping and killing efficiency is greatly improved, and a scientific basis is provided for precise prevention and control of pests such as fruit borers in orchards; the orchard pest trapping and natural enemy conservation device can also trap larvae and pupae of various pests, and the number of orchard pests is remarkably reduced. Meanwhile, the method can provide nutrition support for natural enemies of pests, and the biological pest control function can be better played. Compared with a traditional prevention and treatment scheme, the use cost is lower, the prevention and treatment effect is better, and environment friendliness is achieved.

The application belongs to the technical field of pest control, and discloses application of ceramide synthase 6 gene as a target point in pest control, and specifically discloses application of the CerS6 gene as a target point in control of insects in the order Lepidoptera. The application discloses, for the first time, application of the ceramide synthase CerS6 gene as a target point in control of insects in the order Lepidoptera, and proves importance of the ceramide synthase CerS6 to growth and development and reproductive capacity of pests by screening of a target gene and knocking out the ceramide synthase CerS6 of Spodoptera litura through the CRISPR / Cas9 technology, so as to make a great contribution to biological pest control.

The present invention relates to the technical field of biological pest control, and provides the application of a sex attractant analogue in trapping Spodoptera. The present invention uses trans-9-tetradecene-2,2-difluoroacetate as a sex attractant analogue to trap Spodoptera, which has the advantages of strong trapping ability, small dosage, good stability, and long lasting effect. In addition, the preparation method of trans-9-tetradecene-2,2-difluoroacetate is simple, low cost, and suitable for large-scale use. At the same time, the sex attractant analogue has strong specificity, does not produce resistance, does not harm natural enemies, is non-toxic to humans and animals, and is environmentally friendly. When applied to the prevention and control or monitoring of Spodoptera, accurate Spodoptera insect infestation monitoring can be performed, and a large number of trapping and mating interference can be achieved, which is of great significance for the comprehensive management of Spodoptera.

The invention belongs to the technical field of insect attractants. More specifically, the invention provides an attractant for bactrocera dorsalis, which comprises ethyl acetate, butyl butyrate and dipentene. The attractant provided by the invention can effectively reduce the population density of adult citrus fruit flies in the field. The attractant developed by the invention is a novel bactrocera dorsalis attractant, the raw materials of the attractant are low in cost, wide in acquisition channel, simple in formula and simple and convenient in preparation process, and the attractant has an excellent attraction effect on bactrocera dorsalis. The attractant provided by the invention can provide powerful support for the control work of bactrocera dorsalis, enriches compound resources for biological control of bactrocera dorsalis pests, promotes the comprehensive control strategy of pests to develop towards the directions of high efficiency, economy, greenness and the like, and has very high application value.

The present application relates to the field of biological pest control, and particularly relates to a method for artificially mass and rapid propagation of Drosophila suzukii Coptera. The method comprises the following steps: 1) preparation of Drosophila biarmipes and Drosophila suzukii Coptera feeding tank; 2) collection of Drosophila biarmipes pupae; 3) parasitization of Drosophila suzukii Coptera on Drosophila biarmipes pupae; 4) eclosion and feeding of Drosophila biarmipes pupae parasitized by Drosophila suzukii Coptera; 5) feeding of Drosophila suzukii Coptera adults; and 6) repeating steps 1) to 5), so that a large amount of Drosophila suzukii Coptera can be obtained in a short period of time. The method provided by the present application is time-saving, labor-saving, space-saving, and requires simple devices, and can mass propagate Drosophila suzukii Coptera in a short period of time, so as to meet the application requirements of biological characteristic research test or field control of Drosophila suzukii.

The invention relates to the technical field of biological prevention and control of pests, and provides application of a sex attractant analogue in trapping argyrogramma agnata. According to the invention, trans-9-tetradecene-2, 2-difluoroacetate is adopted as a sex attractant analogue to trap the argyrogramma agnata, so that the sex attractant has the advantages of strong trapping capability, small dosage, good stability and long lasting period; moreover, the trans-9-tetradecene-2, 2-difluoroacetate is simple in preparation method, relatively low in cost and suitable for large-scale use; meanwhile, the sex attractant analogue is high in specificity, free of resistance, harmless to natural enemies, non-toxic to people and livestock and environmentally friendly, when the sex attractant analogue is applied to prevention or monitoring of the argyrogramma agnata, the insect situation of the argyrogramma agnata can be accurately measured and reported, mass trapping and mating interference can be achieved, and the sex attractant analogue has important significance on comprehensive treatment of the argyrogramma agnata.

The present invention provides a method for raising and controlling the natural enemy of bark beetles, the flat grain beetle, which relates to the technical field of pest biological control and solves the technical problem that the flat grain beetle is difficult to raise on a large scale. The raising method includes a raising device, which is equipped with a breeding matrix. The materials of the breeding matrix include wheat, flour, oats, glutinous rice, bran and distiller's yeast, wherein: the wheat is cracked wheat, the volume ratio of wheat is 20-40%, the volume ratio of flour is 20-30%, the volume ratio of oats is 20-30%, the volume ratio of glutinous rice is 20-30%, the volume ratio of bran is 5-10%, and the volume ratio of distiller's yeast is 0.5-5%. In the raising device, the humidity of the breeding matrix is ​​easy to control and not easy to rot, which is conducive to the large-scale breeding of the flat grain beetle; when the pest bark beetle occurs before or when the number is small, the predatory natural enemy flat grain beetle is slowly released in stages and batches, which can keep the pest population under control at a low level for a long time.

The utility model discloses a small pest pathogenic bacteria spreading device, and relates to the technical field of pest biological control. Comprising a container 1, a cylindrical cover 2 or a conical cover 3, a pathogenic bacteria carrier frame 4, an attractant carrier mesh bag 5, a moisturizing device 6 and a suspension bracket 7. The device is high in pertinence, target pests are attracted to enter the device through a specific attractant, and the influence on non-target organisms is reduced; the insecticidal composition has a good lasting effect, can be spread in pest populations by contacting pests with pathogenic bacteria, and can control the pests for a long time. The device can be used as a part of comprehensive pest control (IPM), is combined with other prevention and control strategies of pests, and can effectively improve the prevention and control effect of the pests.

The invention belongs to the technical field of biological insect prevention, and particularly relates to a plutella xylostella repellent and application thereof. The coumarin, the 6, 7-dihydroxy coumarin and the trans-anethene provided by the invention have oviposition repelling and repelling activity on the plutella xylostella, in addition, the repelling duration of the trans-anethene can reach 8 days, and a basic support is provided for developing a chemical repellent preparation for field control of the plutella xylostella. According to the scheme, a monitoring tool based on chemical information is improved, and direct and efficient pest control is achieved. The research highlights the important effect of the plant-source volatile matter in controlling the plutella xylostella population, and the potential application value of the plant-source volatile matter as a pesticide material. In future research, behavior regulation and control characteristics of functional traditional Chinese medicinal materials need to be deeply explored, and a chemical ecological mechanism of a three-level nutrition relationship of traditional Chinese medicinal materials, pests and natural enemies needs to be analyzed, so that the effectiveness of a pest control strategy is remarkably improved.

The invention discloses a microbial ecological planting method for rice, and particularly relates to the technical field of rice planting, which comprises the steps of seedbed weeding, biological seed dressing, sowing and seedling raising, seedling raising management, weeding before rice transplanting, wheat straw decomposition, base fertilizer application, topdressing, biological disease control and biological pest control. A functional microbial agent is introduced to replace a chemical pesticide, a soil-crop synergistic micro-ecological regulation and control system is constructed, on one hand, nitrogen-fixing bacteria, phosphate solubilizing bacteria and other microbial communities can activate soil nutrients, and the use amount of chemical fertilizer is reduced by 30% or above; on the other hand, the antagonistic bacteria group inhibits pathogenic bacteria breeding through a space occupying effect, and soil-borne diseases are prevented and controlled from the source.

The invention relates to the technical field of biological prevention and control of pests, and discloses an artificial breeding method of brachycera majorana and a method for biological prevention and control of teak camel moths. According to the method, the Helicoverpa armigera pupae are selected as the alternative hosts of the Brachymelaasus, and meanwhile, the breeding conditions are regulated and controlled, so that the artificial large-scale breeding of the Brachymelaasus is realized, and the Brachymelaasus is artificially released to prevent and control teak camel moths.

The utility model relates to the technical field of biological control of tobacco diseases and insect pests, and particularly discloses an automatic mummified aphid collecting production line which comprises an automatic feeding line, an automatic mummified aphid picking and selecting machine, a mummified aphid output machine, a mummified aphid mixing machine and an automatic mummified aphid racking machine. An output shaft of the first gear motor is rotatably connected with a roller of the partition transmission belt, the first cylinder is arranged on one side of the partition transmission belt, the end of a piston rod of the first cylinder is connected with the push plate, and the extending direction of the piston rod of the first cylinder is perpendicular to the conveying direction of the partition transmission belt. The automatic mummified aphid picking and selecting machine comprises a feeding component, a screen drum, a first brush, a waste seedling outlet, a mummified aphid collecting component and a mummified aphid output port, the feeding component is arranged on the side, away from the first air cylinder, of the partition plate transmission belt, a feeding port of the screen drum is communicated with the feeding component, and the screen drum is driven to rotate through the arranged transmission component.