53 results about "Diamondback moth" patented technology

The present invention relates to a pyrido[1,2-a]pyrimidine mesoionic derivative containing a 1,2,4-oxadiazole structure and a preparation method and use thereof. The compound has a structure of formula (I), has excellent insecticidal activity against bean aphids, white-backed plant hoppers, diamondback moths, fall armyworms, striped stem borers, armyworms, etc., and can be used to prepare drugs or medicaments for preventing and controlling pests such as aphids, rice plant hoppers, diamondback moths, fall armyworms, striped stem borers, armyworms, etc. The compound has a simple structure and preparation process, and has low production cost.

The invention relates to the technical field of compound preparation, in particular to chiral pyrethroid as well as a preparation method and application thereof. The structural general formula of the chiral pyrethroid is shown as a formula I or a formula II. The synthetic method has a wide substrate range, can successfully prepare a single configuration of a pyrethroid product and test the biological activity of the pyrethroid product, is simple and convenient to operate and high in yield, allows an optical pure isomer to reach 85% or more, prepares the pyrethroid product by a one-pot multi-step method, ensures the ee value and yield of the single configuration, and has a wide application prospect. The whole activity of the compound on plutella xylostella and spodoptera frugiperda is shown as 1R-cisgt; rac, cisgt; according to the present invention, the pyrethroid is a pyrethroid product, the 1S-cis is the low-efficiency body or the ineffective body of the pyrethroid product, and has wide application prospects in the academic field and the pesticide industry.

This invention relates to the fields of pesticide formulations and pesticide pest control, specifically to a composite nano-slow-release suspension of emamectin benzoate and chlorantraniliprole, its preparation, and application. The nano-slow-release suspension comprises emamectin benzoate, chlorantraniliprole, a nanocarrier, a wetting agent, a dispersant, a synergist, a thickener, an antifoaming agent, an antifreeze, a preservative, and water. The nanocarrier is an NX-CMCS & MOF composite nanocarrier. Spraying this composite nano-pesticide onto crops improves the adhesion and penetration of the pesticide on leaves, enhances its resistance to rain washout, and effectively kills major crop pests such as bollworms, diamondback moths, cowpea thrips, and rice planthoppers. Compared with traditional pesticide formulations, it has a significant effect of reducing dosage and increasing efficacy, and has broad application prospects in the field of agricultural pest control and promoting green agriculture.

The present invention provides an application of tea tree-derived transcription factor CsWRKY71 in improving plant insect resistance and preparing plant insect-resistant varieties, belonging to the field of biological control technology. The nucleotide sequence of the tea tree-derived transcription factor CsWRKY71 is shown in SEQ ID No. 1, and the gene can play a key role in improving plant insect resistance. Studies have shown that the feeding preference of diamondback moth in Arabidopsis overexpressing the CsWRKY71 gene is significantly reduced; compared with the control, the weight gain of tea geometrid on tea trees overexpressing the CsWRKY71 gene is significantly reduced. That is, the insect resistance of plants overexpressing the CsWRKY71 gene is significantly enhanced. The present invention can provide genetic resources and technical means for the breeding and application of new insect-resistant plant varieties and the green prevention and control of pests, and has potential application prospects and value.

The present invention relates to Brassica oleracea plants resistant to the plant pest diamondback moth Plutella xylostella and, additionally, tolerant to the plant pathogen Xanthomonas campestris pv. campestris. The present invention further relates to methods for identifying the present plants and nucleic acid sequences allowing characterizing, or identifying, the present plants. Specifically, the present invention relates to Brassica oleracea plants resistant to the diamondback moth Plutella xylostella and the plant pathogen Xanthomonas campestris campestris, wherein said resistance to the diamondback moth Plutella xylostella and said tolerance to the plant pathogen Xanthomonas campestris campestris are obtainable from a Brassica oleracea plant deposited under deposit number NCIMB 43822.

This invention discloses a composition containing isoxaflutole and lambda-cyhalothrin, and its uses. The insecticide composition comprises isoxaflutole and lambda-cyhalothrin, with a mass fraction ratio of 1:1-50 between the two active components. Within a certain ratio range, the composition of this invention exhibits a significant synergistic effect against diamondback moth and flea beetles, which is beneficial for achieving reduced pesticide use and increased efficiency, meeting the requirements of the green and environmentally friendly trend in pesticides.

According to the preparation method of the Chinese honeylocust fruit extract botanical surfactant, the quality and the yield of Chinese honeylocust fruit powder are remarkably improved through the processes of smashing, decolorizing and the like, and the prevention and treatment effect of Oxazol sufu on plutella xylostella and brown planthopper is improved through a composition with the obtained Chinese honeylocust fruit extract and Oxazol sufu as active ingredients.

This invention discloses aryl heterocyclic compounds containing substituted unsaturated hydrocarbons, their preparation methods, and uses. The compounds have the chemical structure shown in Formula I. The compounds of this invention are used as insecticides, showing particularly good control efficacy against armyworms, diamondback moths, cotton bollworms, corn borers, and rice leaf rollers, and are expected to become novel insecticides.

The present invention belongs to the technical field of pharmaceutical synthesis, and particularly relates to epiandrosterone pyrazole derivatives, their synthesis methods and applications. The epiandrosterone pyrazole derivatives have a chemical structure represented by the general formula (1), wherein R is an alkyl group or an aromatic ring-containing group. The alkyl group is an aliphatic chain or a cycloalkane, and the aromatic ring-containing group is a phenyl group or a substituted phenyl group. The synthesis method of the epiandrosterone pyrazole derivatives uses epiandrosterone as a basic raw material and obtains the epiandrosterone pyrazole derivatives of the present invention through a series of different reactions. The epiandrosterone pyrazole derivatives disclosed in the present invention are a series of brand-new compounds, which are proposed for the first time. The synthesis method has a high preparation yield, and the obtained product is easy to separate. It is confirmed by biological assays that the epiandrosterone pyrazole derivatives exhibit good insecticidal activity against pests such as aphids, oriental armyworms, diamondback moths and whiteflies, and can be applied to the control of plant pests.

The present invention provides a chitin synthesis inhibitor compound-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is buprofezin, lufenuron, cyromazine, diafenthiuron, diflubenzuron, chlorbenzuron, hexaflumuron or chlorfluazuron, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially brown planthopper, beet armyworm or liriomyza sativae. The invention verifies that the binary composition of the inhibitor insecticide synthesized from the flufenutraniliprole and the chitin has a remarkable synergistic effect, effectively improves the control effect, reduces the dosage and expands the insecticidal spectrum. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I).

This application discloses a Penicillium strain with stress resistance, growth promotion, and insecticidal functions, and its applications. The preservation number of this Penicillium strain is CGMCC No. 42545. This Penicillium strain naturally integrates four functions: salt tolerance, drought tolerance, plant growth promotion, and insecticidal activity against lepidopteran pests. It can grow normally in a 15% NaCl concentration medium, significantly improving plant survival rate under drought stress. Simultaneously, it promotes growth indicators such as root length and root fresh weight in crops like peas. It exhibits highly effective lethal activity against young larvae of lepidopteran pests such as diamondback moth and beet armyworm, and has high safety against non-target organisms. This Penicillium strain can be prepared into solid or liquid inoculants, showing promising application prospects in stress resistance, growth promotion, and pest control for crops grown in saline-alkali / arid areas. It realizes an integrated solution of "stress adaptation - crop growth promotion - pest control," replacing the combined use of multiple single-function agents and reducing agricultural production costs.

The invention discloses an insecticidal preparation based on rosemary-mint mixed oil and a preparation method and application thereof, and belongs to the crossing field of green agriculture and biotechnology. The mixed essential oil is efficiently extracted through an ultrasonic-subcritical coupling extraction process, the rosemary oil extraction rate can reach 12.1%-12.4%, the menthol retention rate reaches 95.2%-97.1%, and the solvent residue is lower than 0.01 mg / kg; a full-plant-source ternary compound system (mixed essential oil, sophora alopecuroide extract and citrus peel oil) is designed, the synergistic coefficients of 50 formulas are all larger than or equal to 1.25, and six types of pests including thrips, red spiders, aphids, whiteflies, plutella xylostella and leafhoppers can be covered; a special application technology for eight types of crops (including tea leaves and traditional Chinese medicinal materials) is established, the quality of the crops reaches the standard after pesticide application, for example, the water extract of the tea leaves is increased by 1.2%, and the astragaloside content meets the standard of Chinese Pharmacopoeia 2020 edition; the storage stability is optimized, the control effect retention rate can reach 94.7% after 6 months of normal-temperature storage, and the requirement of 2-year industrial shelf life can be met; finally, the average reduction rate of the chemical pesticide is 90.3%, and the preparation cost is less than or equal to 80 yuan / kg. The composition can replace chemical pesticides and is suitable for green prevention and control of insect pests of fruits and vegetables, fields, commercial crops and traditional Chinese medicinal materials.

The present invention discloses the application of rice leaf extract in insecticidal use. The present invention provides the application of rice leaf extract in any one of the following: insecticidal use; preparation of insecticides. The rice leaf extract provided by the present invention has a certain control effect on aphids, diamondback moths and spider mites. The present invention has important significance for controlling these three pests on crops.

The invention discloses an Enterobacter holmesii A3 strain and its application, and the strain is preserved in the Guangdong Provincial Microbial Culture Collection Center, and the biological preservation number is: GDMCC NO: 64603. The Enterobacter holmesii A3 strain of the invention can improve the production of resistance of diamondback moth, and the strain can survive in a culture medium containing only bromofenac or chlorfenapyr as the only nutrient source, and can degrade the pesticide in vitro; the Enterobacter holmesii A3 strain of the invention provides a basis for the study of the resistance mechanism of diamondback moth, and at the same time, because it has the application in pesticide degradation, it provides a basis for the application of biological control.

The invention provides application of mustard oil in preparation of a pesticide for preventing and treating cruciferous pest plutella xylostella. The invention further provides the Pickering emulsion gel containing the mustard oil, and the invention further provides the Pickering emulsion gel containing the mustard oil. According to the preparation method of the green pesticide mustard oil Pickering emulsion gel provided by the invention, the mustard oil is entrapped in the nano-carrier to form the stable Pickering emulsion gel. The compound is simple in preparation process, green and environment-friendly, high in safety and stability and relatively high in killing power to pests, has antibacterial activity as a green pesticide, is green and efficient, is easily degraded by the environment, can remarkably improve the pesticide efficiency and reduce the environmental influence, and solves the problems of high toxicity, difficulty in degradation and low utilization rate of the existing pesticide.

The invention belongs to the technical field of gene engineering, and particularly relates to a method for knocking out a brassica napus BnaFAH gene by utilizing a CRISPR-Cas9 system and application. Two copies of the brassica napus BnaFAH gene are knocked out at a fixed point by using a CRISPR-Cas9 system, two target fragments are selected from conserved regions of fourth and sixth exons of the two copies of the brassica napus BnaFAH gene, BnaFAH-Cas9 plant expression vectors are respectively constructed, the BnaFAH-Cas9 plant expression vectors are introduced into brassica napus hypocotyl callus through an agrobacterium infection method, and seedlings are regenerated. Two copies of the BnaFAH gene are subjected to double-strand shearing under the combined action of two sgRNAs and Cas9 nuclease, random deletion on a target gene segment is finally realized through a cell self-repairing function, gene knockout is completed, a mutant plant is obtained, the mutant plant obtained after the BnaFAH gene is knocked out is verified to enhance the resistance to plutella xylostella, and the mutant plant has a good application prospect. Therefore, the gene is a key gene for regulating and controlling the insect resistance of the brassica napus, and has important significance for breeding new insect-resistant varieties of the brassica napus.

This invention provides a method for preparing and applying a controlled-release agent of emamectin benzoate, belonging to the field of nanopesticide technology. This invention uses PFC-1, a hydrogen-bonded organic framework material with a porous structure, as a drug carrier. After loading emamectin benzoate through its porous structure, the loaded system is further modified by surface encapsulation with sodium lignosulfonate. The resulting nano-formulation with controlled-release function is called emamectin benzoate nano-controlled-release agent. The prepared drug-loaded system exhibits good insecticidal activity against diamondback moth. The preparation method includes a method for preparing emamectin benzoate-loaded agents. The prepared system has a high loading capacity of 19.6%. The drug-loaded system provided by this invention has good insecticidal effect against diamondback moth and exhibits pH-selective release effect in the midgut environment of the diamondback moth.

The invention relates to the technical field of preparation of phenylisoxazole derivatives, in particular to phenylisoxazole derivatives containing pyrazole or triazole amide as well as a preparation method and application of the phenylisoxazole derivatives containing pyrazole or triazole amide. The phenylisoxazole derivative containing pyrazole or triazole amide provided by the invention has good insecticidal activity on lepidoptera pests such as plutella xylostella, spodoptera frugiperda, beet armyworm, cotton bollworm and the like, and can be used as a pesticide active component to be applied to prevention and control of agricultural pests and preparation of insecticides.

The present invention provides a multifunctional Bacillus thuringiensis LT2016 and its application, belonging to the technical field of biocontrol bacteria. The Bacillus thuringiensis LT2016 of the present invention is deposited in the General Microbiology Center of the China Microorganism Culture Collection Committee with a deposit number of CGMCC No. 28725 and a deposit date of October 24, 2023. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The Bacillus thuringiensis LT2016 of the present invention has three functions: preventing and controlling lepidopteran pests such as diamondback moth, inhibiting fungal growth, and promoting corn growth and increasing yield. The microbial agent prepared using the biocontrol bacteria can reduce the amount of other chemical pesticides, reduce the cost of corn disease prevention and control, promote corn growth, and has good promotion value.

The application discloses a class of evodiamine derivatives targeting insect ryanodine receptors and preparation and application thereof. The compound of the application has a structural formula as shown in I, wherein R, R 1 , R 2 are defined in claim 1. The compound of the general formula I of the application has medium to excellent insecticidal activity on pests of Lepidoptera such as oriental armyworm and diamondback moth, and research on the insecticidal mechanism finds that it is a class of insect ryanodine receptor activators. The application synthesizes a class of insecticides targeting insect ryanodine receptors, which can be used alternatively with existing insecticides to avoid or delay the generation of resistance, has conventional preparation conditions, simple subsequent treatment and is easy to realize industrialization, and is an insecticide with wide application prospect.

This invention belongs to the field of pesticide chemistry. This invention provides an isoxazoline pyridazine derivative, which is a compound represented by general formula I or II or its stereoisomers, polymorphs, isotope labels, or pharmaceutically acceptable salts. The isoxazoline pyridazine derivative of this invention exhibits significant insecticidal activity and broad spectrum, achieving a corrected mortality rate of 23.81%–100.00% against diamondback moth, 17.78%–88.89% against Asian corn borer, and 20.00%–93.33% against peach aphid. The isoxazoline pyridazine derivative of this invention also shows LC50 activity against zebrafish. 50 ≥10 mg / L, which falls under the low-risk category for aquatic organisms and has low environmental risk.

The application provides an isoxazoline oxime ester derivative, a preparation method and application thereof, and the isoxazoline oxime ester derivative is a compound represented by formula I, or a stereoisomer, a tautomer, an isotopic derivative and a pesticide-acceptable salt thereof. The isoxazoline oxime ester derivative has high insecticidal activity, still has good insecticidal effect at a low dose, is more conducive to environmental protection, and has very excellent killing effect on lepidopteran pests, such as diamondback moth.

The present invention relates to the technical field of pesticides, and in particular to an insecticide comprising methoxazole and pyrimidine and an application thereof. The mass ratio of methoxazole to pyrimidine is 2:8-7:3. The insecticide provided by the present invention has an obvious synergistic effect on cabbage aphids and diamondback moths, can reduce the amount of medicaments used, reduce costs, reduce harm to the environment, and can effectively delay the generation of pest resistance.

The present invention relates to the technical field of pharmaceutical synthesis, and specifically relates to diene thiazole derivatives and their synthesis methods and applications. The diene thiazole derivatives disclosed in the present invention are a series of brand-new compounds, which are proposed for the first time. The diene thiazole derivatives have the chemical structure shown in general formula (1). The synthesis method of the diene thiazole derivatives of the present invention uses 16-DPA as the basic raw material and obtains the diene thiazole derivatives of the present invention through a series of different reactions. The synthesis method has a high preparation yield, and the obtained product is easy to separate. It has been confirmed by biological assays that the diene thiazole derivatives exhibit good insecticidal activity against pests such as aphids, oriental armyworms, diamondback moths, and whiteflies, and can be used to prepare single-agent agricultural insecticides or mixed preparations containing the diene thiazole derivatives. #imgabs0#

The invention provides a plutella xylostella fibroin light chain gene, which has a nucleotide sequence as shown in SEQ ID NO: 1 and an amino acid sequence as shown in SEQ ID NO: 2. The invention further provides application, and the plutella xylostella fibroin light chain gene is used for preventing and treating plutella xylostella mediated by a CRISPR / Cas9 gene editing system and is used for developing a pesticide target gene. After the plutella xylostella fibroin light chain gene is used for inducing the mutation of a plutella xylostella target gene by using a CRISPR / Cas9 technology, the plutella xylostella is not cocooned into naked pupa, which indicates that the fibroin light chain gene plays a key role in the synthesis of fibroin of the plutella xylostella. The plutella xylostella silk fibroin light chain gene mutation is induced based on the CRISPR / Cas9 technology, the synthesis of the plutella xylostella silk protein is blocked, and meanwhile, the death rate of larvae is increased. The gene not only can be used for pest control mediated by a CRISPR / Cas9 gene editing system, but also can be used for research and development of a novel insecticide target gene.

The present invention discloses a pesticide insecticide containing brofenac and fenthiocarb. The pesticide insecticide includes active ingredients, wherein the active ingredients are brofenac and fenthiocarb; the mass ratio of brofenac to fenthiocarb is 1:20 to 20:1. Due to the specific selection of brofenac and fenthiocarb for compounding, the two can produce a synergistic effect, thereby making the formed pesticide insecticide have good insecticidal effects and a wide control spectrum, especially for lepidopteran pests such as diamondback moth, gypsy moth, and dipteran Culex pipiens pallens.

The invention provides a method for performing a bioassay experiment on plutella xylostella by using artificial feed, and belongs to the technical field of pesticides. The bioassay experiment method comprises the following steps: preparing an artificial feed; the method is actually used for operation steps of plutella xylostella. According to the experiment, after the artificial feed is prepared, the artificial feed is prepared into small blocks by a puncher, and the small blocks are put into a solution to be soaked, so that compared with a traditional bioassay experiment method, the effect that the plutella xylostella larvae eat actual soaked substances in a bioassay cup can be ensured, and the accuracy of the experiment is improved.

The invention relates to the field of insecticides, in particular to a benzodioxane compound and / or salt thereof as well as a preparation method and application of the benzodioxane compound and / or salt thereof. The invention provides a benzodioxane compound which is novel in structure and good in comprehensive performance. The benzodioxane compound has the structure as shown in the formula I in the specification. The benzodioxane compound has an insecticidal function and can be used for preventing and treating main pests in agriculture, forestry and gardening. The benzodioxane compound provided by the invention is used as an active component as an insecticide, and has an excellent control effect on pests such as plutella xylostella and the like when the benzodioxane compound is used at a low concentration content. # imgabs0 #

The invention belongs to the technical field of microorganisms, and particularly discloses bacillus cereus D9 and application thereof in prevention and treatment of lepidoptera pests. According to the bacillus cereus D9 provided by the invention, the Latin name is bacillus cereus, and the preservation number of the bacillus cereus D9 is CGMCC (China General Microbiological Culture Collection Center) No.37192. The strain can significantly reduce the survival rate of second-stage larvae of tomato leaf miners and weaken the oviposition preference of imagoes in host tomatoes; the obvious inhibition effect is also realized on the survival of second-stage larvae of the plutella xylostella. Through synergistic interaction of killing larvae and weakening the oviposition preference of adults, efficient prevention and control of lepidoptera pests can be achieved, chemical pesticide application can be reduced, the requirements for green prevention and control and agricultural sustainable development are met, and the method has important practical value for guaranteeing the yield and quality of crops and protecting the ecological environment of farmlands.

The present invention discloses N-(1-arylpyrazolylmethyl-substituted pyridin-2-ylidene)amide compounds, a preparation method thereof and an application thereof. The general structural formula of the compounds is shown in Formula I, wherein R 1 is hydrogen, a C1-C5 saturated or unsaturated aliphatic hydrocarbon group, a halogen, a haloalkyl group, a cyano group or a nitro group, etc. R 2 is hydrogen, a halogen mono-substituted or multi-substituted at the 1, 2 or 3 position, a hydroxyl group, a methoxy group, a haloalkyl group, a cyano group or a nitro group, etc. R 3 is a haloalkyl group, a C1-C5 alkyl group, a C1-C5 alkoxymethyl group, an aryl group or a substituted aryl group, etc. Insecticidal activity experiments show that the arylpyrazole compounds shown in Formula (I) have high control efficacy against crop pests such as planthoppers and diamondback moths, and can be used as plant insecticides.