99 results about "Mythimna separata" patented technology

The invention relates to a method for controlling eastern mythimna separata injurious insects. The method comprises the step as follows: the eastern mythimna separata injurious insects contact Cry1A protein. The eastern mythimna separata injurious insects are controlled by producing the Cry1A protein capable of killing the eastern mythimna separate inside a plant body. Compared with an agricultural prevention method, a chemical prevention method and a physical prevention method used in the prior art, the whole growth period and whole plant protection is carried out on the plant, so as to prevent the damage of the eastern mythimna separata injurious insects. The method is free of pollution, free of residue, stable and thorough in effect, simple, convenient and economical.

The invention discloses an insect-fusion-resistant gene, coding protein, a carrier and application thereof. The insect-fusion-resistant gene is formed by using a connecting peptide coding gene to connect a nucleotide sequence of coding BT insecticidal crystal protein Cry1Ab with coding BT trophophase insecticidal protein Vip3A, the connecting peptide amino acid sequence is shown as SEQ ID NO:1. Insecticidal activity of the insect-fusion-resistant gene is improved, and insecticidal spectrum is wider. The insect-fusion-resistant gene can kill main lepidopterous insects like armyworm, Spodoptera litura, Ostrinia furnacalis, cotton bollworm and Spodoptera exigua of paddy and maize. In addition, the insect-fusion-resistant gene can effectively slow down insect resistance.

The present invention discloses a broad spectrum pesticide extracted by using plant composition as raw material and its preparation method. Said plant composition is made from celastrus angulatus, melia azedarach, melia toosendan, acorus calamus, euphorbia fischeriana, aconitum cermichaeli and zanthoxylum armatum, and their extracts include water extract, acid extract, ether extract and alcohol extract, etc. Said invention can effectively kill several pests of grasshopper, red spider, armycworm, pine moth and others. Said extration liquor is acetic acid perfectly, and its extraction temp. is 45-60 deg.C.

Disclosed herein are methods of controlling insect pests which infest crop plants, in particular Spodoptera frugiperda (fall armyworm), Lygus hesperus (western tarnished plant bug), Euschistus heros (neotropical brown stink bug), and Plutella xylostella (diamondback moth), and methods of providing plants resistant to such pests. Also disclosed are polynucleotides and recombinant DNA molecules and constructs useful in such methods, insecticidal compositions such as topical sprays containing insecticidal double-stranded RNAs, and plants with improved resistance to infestation by these insects. Further disclosed are methods of selecting target genes for RNAi-mediated silencing and control of these insect pests.

The invention relates to novel hydrazide derivatives as well as a preparation method and application thereof. The novel hydrazide derivatives have a general formula as shown in the specification, wherein each group is as shown in the claim 1. The novel hydrazide derivatives provided by the invention are improved in insecticidal activity while the previous compound lipid solubility thereof is improved; the derivatives have excellent insecticidal activity; instantly after the derivatives are applied, insects can lose control on muscle and stop eating, and are obviously shrank and paralyzed; at last, the insects can be caused to die, and in the meantime, abnormal ecdysis of insects also can be induced; the novel hydrazide derivatives are very effective especially for lepidoptera pests such as mythimna separata, plutella xylostella and asparagus caterpillar, and thus are pesticides having wide application prospect.

The invention discloses an artificial large-scale armyworm culture method. The method comprises the steps of A, egg mass collection and larva hatch, B, 1-3 age larva feeding, C, 3-5 age larva feeding,D, 5-6 age larva feeding, E, pupation and pupa feeding and F, adult eclosion, mating and spawning. An inventor has been committed to research on the biological nature, predatory function and artificial feeding of natural enemy insects for a long time, under the weather of heavy rain, high humidity and insufficient illumination in Suiyang County of Guizhou province, large-scale armyworm feeding isachieved, the yearly yield reaches 20 million or above, Arma chinensis and Picromerus lewisi Scott in Suiyang County are fed with armyworms, and the Arma chinensis yield reaches one million or aboveand Picromerus lewisi Scott yield reaches 400 thousand or above; the achievement of the natural enemy insects is guaranteed.

The present invention discloses a plurality of indolocarbazole alkaloids having strong insect disinfestation activities, and a production strain of the indolocarbazole alkaloids. The indolocarbazole alkaloid compound production strain CQT14-24 is a marine Streptomyces nitrosporeus CQT14-24 strain, wherein the strain is subjected to artificial culture, and the obtained fermentation product is extracted by the conventional chemical method so as to obtain four indolocarbazole alkaloids and an anthraquinone compound. According to the present invention, the indolocarbazole alkaloids and the one anthraquinone compound provide strong killing effects on a variety of cultivated plant pests, particularly the pests having characteristics of spreading in agriculture and forestry production and serious harm and comprising Pieris rapae, Brevicoryne brassicae, Hyphantria cunea, Plutella xyllostella, Mythimna separate and Helicoverpa armigera, wherein the relative mortality rate achieves 81.9-100%, the polypide is seriously reduced, the growth inhibition rate is 100%, and the imago pupation rate is reduced by 70.4-86.7%; and the strain is subjected to acclimation through the artificial culture, and has characteristics of stable feature, easy culture, simple and feasible operation method, no environmental pollution, short period, cheap raw materials, low production cost, good development prospect, and easy industrialization achieving.

The invention relates to the field of disease and pest control, and provides a green and safe disease and pest control method. Staging control is carried out according to different growth periods of plants. The specific control sequences and steps are as follows: (1), disease and pest control in the early stage comprises: control on damping-off, sheath blight and stubby root nematode before soil is not cultivated, and control on pests having relatively high mobility or needing adults to lay eggs and hatch, such as locusts, armyworms and moths, before plants are planted; (2), disease and pest control in seedling raising and seedling stages comprises: selection and use of disease-resistant varieties and health seedlings, disinfection of seeds and substrates, and control on whiteflies, flea beetles and cutworms in the seedling stage; and (3), disease and pest control in field planting and after field planting comprises: environmental management, water and fertilizer management, and adoption of different botanical fungicides and methods for different diseases and pests. Besides, the invention specifically discloses specific disease and pest control methods of Chinese cabbages, tomatoes, strawberries and hami melons. The green and safe disease and pest control method in the invention has the benefits that: a disease and pest control system is formed; and the method is wide in application range, good in effect and free from pesticide residue.

The invention provides an artificial breeding production method of trichogramma japonicum. The method is high in production efficiency, short in breeding cycle and beneficial to large-scale production of the richogramma japonicum. The method comprises the following steps that 1, sticky eggs are produced; 2, the sticky eggs are dissolved into single particles from an oviposition medium with a neutral protease solution with the concentration being 0.2%-0.5% (g / ml); 3, the sticky eggs treated in the step 2 are used for breeding the trichogramma japonicum. According to the method, the trichogramma japonicum bred artificially is used for preventing and treating agricultural insects, and the agricultural insects mainly comprise rice-stem borer, tryporyza incertulas and rice leaf folder. Compared with the prior art, the method has the advantages that armyworms are simple in source and easy and convenient to breed; the method is high in production efficiency and short in breeding period, large-scale breeding is convenient, and large-scale production of the trichogramma japonicum is promoted.

The invention discloses an insecticidal fusion protein which comprises IPD072 protein and Vip3 protein. Compared with the previous IPD072 protein and Vip3 protein, the designed artificial protein molecule formed by fusing IPD072 protein and Vip3 toxin has the following advantages: the insecticidal spectrum is broad, multiple important pests (such as laphygma exigua, armyworm and diabrotica virgifera) of lepidoptera and coleoptera can be controlled at the same time, and the insecticidal efficiency is as high as 50-100%; and moreover, the joint utilization of proteins with different functions and other insect-resistant proteins (such as ICPs) is promoted, the insecticidal spectrum is further expanded, and the generation of insect resistance is delayed.

The invention relates to a nitrogen (sulfur) containing bridge ring compound with insecticidal activity, a preparation method and an application. Specifically, the invention discloses a compound with a general formula I or an optical isomer, a cis-trans-isomer or an agricultural pharmacology acceptable salt of the compound, and a preparation method thereof, wherein R1, R2, R3, R4, R5, R6, R, m, X and Z are respectively as defined in the specification. The invention also discloses an agricultural composition comprising the above compound and an application thereof. The above compound has high insecticidal activity for homoptera and lepidoptera agriculture and forestry insects, such as aphids, plant hoppers, whiteflies, cotton bollworms, prodenia litura, armyworm, etc. The general formula 1 is shown in the specification.

The invention discloses an alpha-amino phosphonate compound with a 2-amino-1, 3, 4-thiadiazole structure and a preparation method and application of alpha-amino phosphonate compound. The alpha-amino phosphonate compound has the sterilizing effect of alpha-amino phosphonate compounds and has a pesticide effect by introducing the 2-amino-1, 3, 4-thiadiazole group, and the alpha-amino phosphonate compound is wide in application range and good in sterilizing and pesticide effect by combining the alpha-amino phosphonate compound with the 2-amino-1, 3, 4-thiadiazole group. The preparation method is few in step, simple to operate, high in yield and productivity, capable of saving a large amount of time and cost in actual production and good in economic benefit. The 2-amino-1, 3, 4-thiadiazole has an inhibition effect on the growth and proliferation of alternaria solani and on the growth of mythimna separata and can be used as the pesticide intermediate to apply to the preparation of different agricultural fungicide.

The present invention relates to an oxadiazole compound and a preparation method thereof, wherein the molecular formula of the compound is as the follow, R1 is halogen, hydrogen, C1-C4 alkyl or alkoxy, R2 is halogen, hydrogen or alkyl, and R3 is methylamine, dimethylamine, isopropylamine, propylamine, butylamine, morpholine, piperidine or imidazole. Compared with the oxadiazole compound and the preparation method in the prior art, the oxadiazole compound and the preparation method of the present invention have the following characteristics that: the process is simple, the application range is wide, and the oxadiazole compound is suitable for prevention and control of health pests such as flies, mosquitoes, fleas and the like and agricultural pests such as lissorhoptrus oryzophilus, spodoptera exigua hiibner, mythimna separata (walker) and the like, can be provided for inhibiting growth of insects, especially mosquito larvae, and is an insecticide with an application prospect.

The invention provides Bt proteins with pesticidal activity against mythimna separata, and an application thereof. The protein is sourced from bacillus thuringiensis, and is at least one selected from Cry1Ab protein, Cry1Bb protein, Cry1Be protein, Cry2Ab protein, Cry9Aa protein, Cry9Eb protein and Cry9Ee protein. The Bt proteins with high toxicity against mythimna separata assist in filling the lack of mythimna separata controlling resources in the current market. Also, the above proteins provide a novel gene source for the preparation of anti-mythimna separata transgenic crops. The proteins can be used in controlling pest drug resistance against Cry1A products, and in delaying the resistance.

The invention discloses a pyrethroid compound having a structure represented by general formula (I). The invention also discloses a preparation method of the compound, and uses of the compound. The method comprises the following steps: utilizing a chiral adjuvant induced asymmetric syntheses technology to construct a novel pyrethroid key intermediate chiral carboxylic acid, and condensing the chiral carboxylic acid with active molecules comprising plant essential oil micromolecules, substituted cyanohydrin, fluorine-containing benzyl alcohol and the like to obtain the novel pyrethroid compound. The pyrethroid compound is used for preparing pesticides or pesticide active components for killing important agricultural pests comprising cotton bollworms, diamondback moths, aphids, beet armyworms, armyworms and the like, and healthy pests comprising houseflies, Culex pipiens pallens and the like, and can be widely used for the comprehensive control of crop pests.

The invention belongs to the field of toxicological activity of pest control, and particularly relates to a new drug for controlling mythimna separata and a use method of the new drug. The new drug iscyclosporin A, wherein the mythimna separata refers to 2-year-old mythimna separata. The method for preventing and treating the mythimna separata by using the new drug comprises the step of mixing the new drug with an artificial feed to feed the mythimna separata. According to the invention, the cyclosporin A is mixed with the mythimna separata artificial feed, and by setting different doses of the cyclosporin A, the prevention and treatment effect on the mythimna separata is detected after 7 days. The prevention and treatment effect of the cyclosporin A is evaluated, and the prevention and treatment dosage of the cyclosporin A is determined. And a new active functional group is provided for the design of a standby insecticide or new drug for preventing and treating the mythimna separata.

The invention provides preparation of a pyrrole and pyrrolin fused ring compound with insecticidal activity and use of the pyrrole and pyrrolin fused ring compound with insecticidal activity. Specifically, the invention relates to a compound (shown as the following formula) with the general formula (A) or the optical isomer, the cis-trans-isomer or the agriculturally pharmaceutically acceptable salt of the compounds, and a preparation method of the compound. The invention further relates to an agricultural composite containing the compound, or the optical isomer, the cis-trans-isomer or the agriculturally pharmaceutically acceptable salt of the compound and the use of the agricultural composite. The compound and the derivative of the compound are high in insecticidal activity to agriculture and forestry pests with the same mecoptera, lepidoptera and the like such as the aphid, the plant hopper, the aleyrodid, the leafhopper, the thrips, the cotton bollworm, the cabbage caterpillar, the plutella xylostella, the prodenia litura, the armyworm and the like.

The invention discloses a sex attractant for controlling mythimna separata and application thereof. A medicinal component of the sex attractant for controlling the mythimna separata comprises cis-11-hexadecenal and cis-11-hexadecene acetate according to a weight ratio of 96:4, and further comprises cis-9-hexadecenal, wherein the weight ratio of components of the cis-11-hexadecenal, the cis-11-hexadecene acetate and the cis-9-hexadecenal in the medicinal component is 96:4:(0.5-5). The sex attractant can be applied to dynamic monitoring and control of a mythimna separata population; by such a monitoring method, the nature of a migratory insect source can be clearly determined; the monitoring method has the superiority that cannot be achieved by other monitoring methods; during application tothe control, the using amount of a chemical pesticide can be saved, natural enemies are not killed, the environment is not polluted, the ecological balance is not destroyed, and promotion of sustainable control of the mythimna separata in China is facilitated.

The invention discloses an insecticidal gene wherein the nucleotide sequence of the gene is SEQ ID NO: 1. The invention also discloses the insecticidal protein coded by the insecticidal gene, the amino acid sequence of the protein is SEQ ID NO: 2. The insecticidal protein has rather high insecticidal activity to the main agricultural pest insects including Ostrinia furnacalis, beet armyworm and Pyralidae.

The invention discloses an application of a benzamide or acylhydrazone derivative containing a 5-trifluoromethyl-4-pyrazole amide structure in the aspect of insecticidal activity. The general structure formulas are as shown in A and B in the specification, wherein R1 refers to phenyl and 3-chloropyridyl; R2-R5 refer to hydrogen atom, alkyl, hetroatom-containing alkyl, phenyl, benzyl, heterocyclyl and substituted heterocyclyl. The benzamide or acylhydrazone derivative has broad-spectrum insecticidal activity and has excellent preventing and controlling effects on diamondback moth, heliothis armigera, ostrinia nubilalis, armyworm and sanitary pests such as culex pipiens.

The invention relates to an insecticidal composition containing spinetoram and cyantraniliprole. The insecticidal composition is formed by compounding active ingredients including spinetoram and cyantraniliprole in a binary manner, wherein the mass ratio of the spinetoram to the cyantraniliprole is (1:40):(40-1), the total mass of the active ingredients including the spinetoram and the cyantraniliprole in a preparation accounts for 1%-80%, the residual is auxiliary components which are allowable and acceptable in an insecticide, and the insecticidal composition can be prepared into forms such as emulsifiable solution, suspending agent, wettable powder, water dispersible granule, emulsion in water and microemulsion. The insecticidal composition is effective to lepidoptera, diptera, coleopteran, hemiptera, thysanoptera and homoptera insects such as tobacco bud worms, cotton aphid, tetranychus urticae, beet mythimna separata, cabbage looper, sweetpotato whitefly, leaf rollers, cotton bollworms and the like.

The invention discloses a simulated phytotron for Arma chinensis breeding, comprising a house body which is closed inside. A Mythimna separata larva raising device, an Arma chinensis larva breeding device, an Arma chinensis adult feeding device, a temperature control device and a humidity control device are arranged in the house body. The simulated phytotorn aims to solve the problem of low survival rate of Arma chinensis in artificial raising in the prior art.

The invention discloses a high-efficiency attractant for mythimna lorgi and an application of the high-efficiency attractant. The high-efficiency attractant for the mythimna lorgi is prepared from thefollowing components in percentage by mass: 50 to 90 percent of cis-9-tetradecenyl acetate and 10 to 50 percent of cis-7-dodecenyl acetate. The high-efficiency attractant can trap and kill male mythimna lorgi more effectively, reduces the interspecific reproduction rate, is small in dosage and environmentally friendly, can be accurately applied to insect condition forecasting, mass trapping and mating interference, and has important significance in comprehensive treatment on the mythimna lorgi.

The invention discloses a synthetic insecticidal gene mVip3Aa for transgenic insect-resistant plants and a protein for insect-resistant plants. The amino acid sequence of the protein is SEQ ID NO: 1,and the nucleic acid sequence of the coding gene is SEQ ID NO 3. The invention also discloses corresponding vectors and applications of proteins and genes in insect resistance. After the modified mVip3Aa gene of the invention is transferred into a plant, the negative effects of high expression of Vip3Aa gene on plant traits are significantly reduced in the obtained transgenic plant with high expression of mVip3Aa protein, the ratio of high-quality transformants is increased, and the pollen is less affected. Meanwhile, the obtained transgenic plants with high expression of mVip3Aa protein havethe resistance to Mythimna separata, which is better than that with Vip3Aa.

The invention belongs to the field of toxicological activity of pest control, and particularly relates to a drug synergist for preventing and treating mythimna separata and a using method of the drugsynergist. The drug synergist for preventing and treating mythimna separate is cyclosporin A, wherein a medicine is spinosad, wherein the mythimna separata is a two-year-old larva. The method for preventing and treating the mythimna separata by utilizing the drug synergist comprises the following steps: mixing the spinosad with a certain concentration with cyclosporin A, then mixing with an artificial feed of the mythimna separata again, and then feeding. The cyclosporin A disclosed by the invention has a remarkable effect of preventing and treating mythimna separata by the spinosad, and can be used as a synergist for preventing and treating mythimna separata by the spinosad so as to improve the prevention and treatment effect of agricultural pests by the spinosad.

The invention belongs to the field of pharmacy, and relates to a natural sesquiterpene guaiol compound, in particular to a natural sesquiterpene guaiol compound of a formula (I) and a preparation method thereof and application in preparation of a botanical insecticide. Insecticidal experiment results display that the guaiol compound shows eating refusal, contact killing, fumigation and juvenile hormone analog activity on experimental insects, and the acting mode of the guaiol compound is closely related with the using dose; the contact effect LD50 on 4-age armyworms and 3-age plutella xylostella is respectively 0.072 mg / piece, 24 hours and 8.920 mg / piece, 24 hours; the fumigation virulence LC50 on houseflies and 4-age adult armyworms is respectively 3.52 muL / L, 12 hours and 16.95 muL / L, 12 hours; and the results show that the compound has high insecticidal activity and can be used as an insecticidal active ingredient for preparing the botanical insecticide in a form of spray or fumigant and the like.

The invention relates to a 4-phenyl para-aldoxime ether-containing oxazoline compound as shown in the general formula (I), preparation of the compound and an application of the compound in pesticides. The compound represents a novel concise, wide-spectral and high-efficiency insecticidal anti-mite bactericidal herbicide structure type. When used as a novel insecticidal anti-mite bactericidal herbicide, the 4-phenyl para-aldoxime ether-containing oxazoline compound can greatly control oriental armyworm, cotton bollworm, corn borer, plutella xylostella, beet armyworm and mosquito larvae, can greatly control Tetrangychus cinnabarinus, can greatly inhibit ten pathogenic bacteria including cucumber fusarium wilt, Cercospora arachidicola, Physalospora piricola Nose, Alternaria solani, Fusarium graminearumt, late blight of potato, sclerotinia sclerotioru, Botrytis cinerea, rice sheath blight and phytophthora capsici, and can control and eliminate weeds including rape, amaranth, barnyard grass and crab grass, wherein the meaning of R is as shown in the specification.

The invention provides a pinellia ternata extract containing plant pesticide special for corns in saline and alkaline land and a preparation method thereof. The pesticide is prepared from, by mass, 13-20 parts of profenofos, 1-4 parts of pinellia ternata, 0.7-2.5 parts of narrow-leaf mohonia fortunei extract, 1.2-2.5 parts of diethofencarb, 0.5-1.6 parts of scopolamine, 0.7-2.5 parts of copper sulfate, 3-7 parts of an emulsifier, 5-10 parts of an activating agent and 16-23 parts of an organic solvent. The invention further provides application of the pesticide in saline-alkali land for corn planting. The pinellia ternata extract containing plant pesticide has the advantages that the prepared pesticide has the effect of preventing and controlling pests in the corn planting land and has the remarkably preventing effect on common pests in the corn planting land, such as European corn borers, mythimna separata, aphids, corn leaf mites and cotton bollworm. The prepared pesticide has the same preventing and controlling effect on corn plant diseases while preventing and controlling the pests in the corn planting land.