The invention belongs to the technical field of bioengineering, and particularly relates to a salivary gland
secretory protein gene, double-stranded
RNA thereof and application of the salivary gland
secretory protein gene in reduction of the
survival rate of aphids. Wherein the salivary gland
secretory protein genes are Mp5, Mp11, Mp44, Mp45, MpCOO2 and MpE and are used as dsRNA target genes to be applied to the reduction of the
survival rate of aphids, and the
nucleotide sequences of the genes Mp5, Mp11, Mp44, Mp45, MpCOO2 and MpE are respectively shown as SEQ ID NO. 1-6. The specific dsRNA is designed by targeting the
myzus persicae salivary gland key secretory
protein gene, the
survival rate and the
reproductive capacity of the
myzus persicae are remarkably reduced, and the
myzus persicae salivary gland key secretory
protein gene has efficient
lethality and good targeting property, is safe to non-target organisms, is environment-friendly, can replace chemical pesticides, has the advantages of simplicity and convenience in operation, low cost, easiness in field application and the like, and has a wide application prospect. A brand new
biotechnology solution is provided for green and sustainable prevention and control of aphids.