1452 results about "Pathogenicity" patented technology

The invention relates to bacillus, a biocontrol inoculant prepared from the bacillus and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as ZZ1, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the preservation center is numbered as CGMCC No.31374, and the preservation time is July 19, 2024. The bacillus ZZ1 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus ZZ1 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention belongs to the technical field of RNA biological prevention and treatment, and particularly relates to dsRNA for preventing and treating crop anthracnose and application of the dsRNA. The dsRNA is targeted inositol phosphatidylceramide (IPC) synthetase coding gene dsRNA, and is obtained by transcription of a part of sequence (SEQ ID No.1) of an inositol phosphatidylceramide (IPC) synthetase coding gene CaAUR1, the gene segment is a segment of a target gene for preventing and treating crop anthracnose, and the dsRNA obtained by transcription can obviously reduce pathogenicity of mango anthracnose bacteria, and has the effects of keeping mango fresh, preventing and treating anthracnose. And the compound can be used for preventing and treating the crop anthracnose or preparing products for preventing and treating the crop anthracnose.

The invention provides lactobacillus plantarum NSL0125 and application thereof in preparation of a microbial preparation for promoting calcium supplement and growth and development. The lactobacillus plantarum NSL0125 is classified and named as Lactobacillus plantarum and is preserved in the China Center for Type Culture Collection, and the preservation number of the lactobacillus plantarum NSL0125 is CCTCC (China Center for Type Culture Collection) NO: M 2024609. The lactobacillus plantarum NSL0125 is high in safety, free of pathogenicity and capable of fermenting various nutrient substances to supply energy for growth. Experiments prove that the lactobacillus plantarum NSL0125 can be colonized in intestinal tracts for a long time, and meanwhile, the lactobacillus plantarum NSL0125 has unique effects on the aspects of adsorption, transfer, absorption promotion and the like of calcium ions, so that calcium absorption, growth and development of organisms are effectively promoted. The lactobacillus plantarum NSL0125 is high in fermentation level and suitable for large-scale industrial production. A large number of experimental studies prove that the lactobacillus plantarum NSL0125 and the microbial preparation thereof have remarkable effects of promoting calcium supplement and growth and development, have good application value and have good market application prospects.

The invention relates to the field of prevention and treatment of plant filamentous fungal diseases, in particular to an application of a compound NSC-32 (N-[(5Z)-4-oxo-5-(phenylmethylidene-1, 3-thiazolidin-3-yl) pyridin-4-carboxamide) in prevention and treatment of magnaporthe oryzae, and a preparation method of the compound NSC-32 (N-[(5Z)-4-oxo-5-(phenylmethylidene-1, 3-thiazolidin-3-yl) pyridin-4-carboxamide). MoPex19 protein is used as a target spot, a compound NSC-32 is obtained through screening, and it is proved that the compound can inhibit growth of magnaporthe oryzae and reduce pathogenicity of the magnaporthe oryzae. In addition, the application object, the use method, the target specificity and the action mechanism of the compound NSC-32 are further explored, and a new green pesticide and a new thought are provided for preventing and treating the magnaporthe oryzae.

The invention relates to bacillus, a biocontrol inoculant prepared from the bacillus and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as QY2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31373, and the preservation time is July 19, 2024. The bacillus QY2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus QY2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention relates to bacillus velezensis, a biocontrol inoculant prepared from the bacillus velezensis and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus velezensis provided by the invention is bacillus velezensis and is named as JK1, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31375, and the preservation time is July 19, 2024. The bacillus JK1 disclosed by the invention has relatively excellent bacteriostatic action on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus JK1 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention relates to bacillus amyloliquefaciens, a biocontrol inoculant prepared from the bacillus amyloliquefaciens and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus amyloliquefaciens provided by the invention is bacillus amyloliquefaciens and is named as JK2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CMCC No.31376, and the preservation time is July 19, 2024. The bacillus amyloliquefaciens JK2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus amyloliquefaciens JK2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention discloses application of GGTI298 in prevention and control of rice blast, and belongs to the technical field of rice blast prevention and control. According to the technical scheme, the novel medical application of the GGTI298 is disclosed, and the GGTI298 has good antibacterial activity on magnaporthe oryzae and can inhibit conidia germination and appressorium formation of the magnaporthe oryzae so as to influence pathogenicity. The GGTI298 has a prospect of preventing and treating magnaporthe oryzae infection, and a reference basis is provided for development of antibacterial drugs based on a GGTI298 pilot structure.

The invention discloses a specific antibody capable of combining with a coronavirus SARS-CoV-2 and an SARS-CoV spike protein (SARS-CoV spike protein), and an application of the specific antibody capable of combining with the coronavirus SARS-CoV-2 and the SARS-CoV spike protein. The sequences of the CDRs of the antibody disclosed by the invention are as shown in SEQ ID NO. 1 to SEQ ID NO. 6. The antibody provided by the invention comprises CDRs with a specific sequence, can be specifically combined with SARS-CoV-2 and SARS-CoV spike protein, and has a relatively strong binding reaction with spike protein of another highly pathogenic coronavirus MERS-CoV at the same time. The antibody provided by the invention is strong in targeting property and high in binding activity, and the antibody prepared by the preparation method provided by the invention is high in purity and can be prepared on a large scale. On the whole, the antibody disclosed by the invention can be used for developing and optimizing an SARS-CoV and SARS-CoV-2 infection detection method.

The invention discloses a high-pathogenicity porcine reproductive and respiratory syndrome virus mRNA vaccine composition and application thereof. The vaccine composition is selected from any one of the following combinations: (1) an mRNA vaccine composition consisting of a PRRSV GP3 mRNA (messenger ribonucleic acid), a PRRSV GP4 mRNA (messenger ribonucleic acid) and a PRRSV GP5 mRNA (messenger ribonucleic acid); or (2) an mRNA vaccine composition consisting of a PRRSV GP5 mRNA, a PRRSV membrane protein (M) mRNA and a PRRSV nucleocapsid protein (N) mRNA. According to the invention, a high-pathogenicity PRRSV JX-A1 strain is used as a template, and mRNAs capable of synthesizing a plurality of fusion proteins GP345, GP5M and GP5MN and single structural proteins GP3, GP4, GP5, M and N are respectively constructed. After liposome encapsulation, mice are inoculated for immunological evaluation, and results show that humoral immunity and cellular immunity levels of a single mRNA-LNPs mixed group are higher than those of recombinant mRNA-LNPs, and GP5 + M + N and GP3 + 4 + 5 have relatively best effects. The invention provides a new technical means for preventing PRRSV (Porcine Reproductive and Respiratory Syndrome Virus).

The invention relates to the field of biological control, and discloses screening of entomogenous fungi with high pathogenicity to prodenia litura and a compound application of the entomogenous fungi and tetraniliprole. The beauveria bassiana strain with high toxicity to the prodenia litura is collected from a tobacco field in polder town in Jianghua County, Yangzhou City, Hunan Province. The invention further provides an efficient, pollution-free and residue-free insecticide combining the tolfenpyrad and the beauveria bassiana, and the insecticide has a synergistic effect. Research on the compounding effect of the tetratolfenpyrad and the beauveria bassiana shows that the insecticide can show a remarkable synergistic effect after being compounded, the prevention and treatment effect is improved, the use dosage of a single chemical pesticide can be obviously reduced, environmental pollution is reduced, and the problems that an existing pesticide is prone to generating resistance, not obvious in pesticide effect and the like are effectively solved.

The present invention relates to a primer set for discrimination of bacterial species in a probiotic composition and a method for discriminating bacterial species using the same. Specifically, in the present invention, a primer set targeting the ITS (internal transcribed spacer) region between the 16S rRNA and 23S rRNA of probiotic species and pathogenic bacterial species has been constructed, it has been confirmed that 21 probiotic species and 4 pathogenic bacterial species can be discriminated with high resolution and accuracy by performing metagenomic analysis of a probiotic composition using the same, and the primer set can be usefully used to discriminate bacterial species in a probiotic composition.

The invention relates to bacillus subtilis, a biocontrol inoculant prepared from the bacillus subtilis and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as KC2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31372, and the preservation time is July 19, 2024. The bacillus KC2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus KC2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention provides a Villonella enteritidis strain and an application of the Villonella enteritidis strain. The preservation number of the Villonella enteritidis strain is GDMCC No: 65234. The invention also provides a preparation containing the Wehonor enteritidis, a culture method and application of the Wehonor enteritidis as a labeled microorganism or a target spot in preparation of drugs for screening diseases caused by the Wehonor enteritidis. According to the present invention, the virulence gene and the pathogenicity of the Wehonor enteritidis are analyzed, such that the Wehonor enteritidis has the potential pathogenicity for aggravating the inflammatory bowel disease, and the strain provides specificity in the aspects of biological characteristics, pathogenicity and the like compared with the Wehonor micrococcus, such that the cognition of the Wehonor micrococcus in the intestinal tract is new improved, and the Wehonor micrococcus enteritidis can be used for the treatment of the inflammatory bowel disease. Moreover, a new view angle and a new direction are provided for research on the effect of the Wehonor coccus in the intestinal diseases such as IBD (Infectious Bursal Disease).

The invention belongs to the technical field of molecular biology, and particularly relates to a nucleic acid sequence combination for simultaneously detecting various pathogenic vibrios, an RT-PCR (reverse transcription-polymerase chain reaction) kit and a use method of the RT-PCR kit, and the nucleic acid sequence combination is shown as SEQ ID No.1-SEQ ID No.19. The RT-PCR kit comprises RT-PCR primer probes, the primer probes comprise forward and reverse amplification primers and detection probes respectively aiming at vibrio cholerae, vibrio parahaemolyticus, vibrio fluvialis, vibrio mimicus, vibrio vulnificus and vibrio alginolyticus, and corresponding sequences in SEQ ID No.1-SEQ ID No.19 are adopted. The kit disclosed by the invention can be used for rapidly detecting and identifying the six vibrios at the same time; the kit has high accuracy, specificity and sensitivity, and is suitable for clinical detection of infected samples.

The present invention relates to a computer-implemented multi-module classifier method and system for providing a pathogenicity classification score of a variant of a protein of interest. The classifier comprises a sequence module based on a protein language model (PLM); a structure module based on a graph neural network (GNN); a property module; and a unified head module based on a machine learning model. The invention further relates to methods for preparing, training, and implementing the multi-module classifier system.

The invention belongs to the technical field of genetic engineering and agricultural biology, and provides application of dsRNA for specifically inhibiting RpHsp22.6 gene expression in preparation of insecticides, and the nucleotide sequence of the dsRNA is shown as SEQ ID NO.12. The invention further provides a preparation method of the dsRNA. The invention also provides a method for relieving soybean blue syndrome and provides a small heat shock protein RpHsp22.6 of Emblycoris lividipennis. According to the invention, the problem of how to more effectively and more environmentally prevent and treat the plant diseases and insect pests of the Adelphocoris indica is solved, and the effect of reducing the pathogenicity of the Adelphocoris indica to the soybeans is achieved.

The invention relates to the technical field of biomedical data analysis, and discloses a data analysis method and system for genetic disease gene detection and a storage medium, and the method comprises the following steps: collecting a patient sample, and carrying out high-throughput sequencing to obtain original data; performing quality control and comparison processing on the data to generate variation detection data, and calculating variation information amount; suspicious variation sites are automatically screened, variation information amount is analyzed based on information entropy, and key variation is screened according to a preset threshold; performing Bayesian inference analysis on the key variation, calculating pathogenicity probability, and performing pathogenicity judgment based on an ACMG standard; calculating the matching degree of the key variation and the phenotype in combination with the phenotype information of the patient, and screening the variation conforming to phenotype characteristics; submitting the screening result to a doctor for auditing, and generating a final screening result; and generating a standardized gene detection report according to a final result. According to the method, pathogenic variation is efficiently and accurately recognized, and the automation level and clinical application value of genetic disease gene detection are improved.

The invention discloses an evidence optimization method and device based on a Bayesian model, computer equipment and a storage medium, and relates to the technical field of medical care informatics. The method comprises the following steps: acquiring a text file containing a variation classification result; calculating posterior probabilities corresponding to 17 evidence combinations given by the ACMG / AMP guide, and when selected evidence combination rules meet posterior probability classification, obtaining theoretical pathogenicity probabilities corresponding to the evidence intensity grades; finely classifying the clinical significance unknown variation VUS into six levels; determining thresholds corresponding to different evidence intensity grades by comparing the positive likelihood ratio or the local positive likelihood ratio with the theoretical pathogenicity probability corresponding to each evidence intensity grade; and the variation is marked with an ACMG / AMP evidence label again, and a pathogenicity classification result of the variation is given. According to the method, the evidence optimization difficulty can be reduced, and the accuracy and consistency of genetic variation interpretation in clinical gene detection can be improved.

The present invention relates to the field of microbial self-assembling protein fibrils as novel bionanomaterials. More specifically, the present invention relates to protein nanofibril materials comprising Bacillus endospore appendage (ENA) proteins, which are spontaneously folding helix hairpin subunits assembling into alpha-helical multimeric (A-ENA) fibrils with a hydrophobic core and covalently connected by one or more isopeptidic bonds, providing for a nanofibril of high stability and tensile strength. The invention further relates to said protein nanofibrils which are engineered or modified to provide for functionalized bionanomaterials. In particular, the invention relates to methods for recombinant production of said self-assembling protein nanofibrils, and their use in modifying bacterial endospores and endospore activity or pathogenicity.

The invention relates to a fusion protein, an immunogenic composition and a recombinant degenerate vaccine for preventing and treating infection of various pathogenic streptococci, as well as a molecular architecture design, application and the like. According to the invention, three immune antigens, namely an elongation factor Tu (Tuf), a molecular chaperone DnaK and an elongation factor G (fusA), are screened, and it is proved that fusion protein molecules of the three antigens can significantly inhibit tissue lesions caused by infection of different serotypes and different types of streptococci, have good immunogenicity, play roles in immune protection and effective prevention and treatment, and have good application prospects. The bacillus subtilis has the characteristics of broad-spectrum and high-efficiency prevention of streptococcus bacterial infection, and has a wide application prospect.

The invention discloses an identification and analysis method for unknown pathogenic microorganisms, which comprises the following steps: filtering out genome sequences with low integrity, pollution and tag errors, and establishing a high-quality virus identification database; constructing a virus host prediction model through a machine learning algorithm; unknown pathogenic microorganisms are identified and analyzed, potential hosts or pathogenicity of the unknown microorganisms are identified, and whether the unknown microorganisms are unknown pathogenic viruses or bacteria or not is further judged. On the basis of metagenome data analysis, potential unknown pathogenic microorganisms in samples of human bodies, environments and the like can be identified more accurately.

The invention belongs to the technical field of biological control, and particularly relates to a streptomyces bacteriophage St01 and application thereof. The streptomyces bacteriophage St01 is separated from a soil environment, has the advantages of relatively good propagation performance, short outbreak period, relatively good environmental stability, good genetic stability and good safety, can specifically infect and split pathogenic streptomyces without killing other bacteria, does not cause additional influence on normal flora, and has a good application prospect. As a bacterial virus, the bacteriophage is not influenced by bacterial drug resistance, does not cause the problems of drug residues and the like in application, and has the characteristics of high safety and environmental friendliness; the bacillus subtilis can efficiently kill pathogenic streptomycete in plants and in a soil environment, and has good application prospects in killing the pathogenic streptomycete in the environment, preventing and treating pathogenic streptomycete infection and preventing and treating plant diseases caused by the pathogenic streptomycete; meanwhile, the method can also be used for detecting pathogenic streptomyces and / or identifying plant diseases caused by the pathogenic streptomyces.

The invention relates to the technical field of microorganisms, in particular to animal-derived pediococcus acidilactici and application thereof. The pediococcus acidilactici is classified and named as pediococcus acidilactici, the strain number of the pediococcus acidilactici is 12NN1, the pediococcus acidilactici is preserved in Guangdong Microbial Culture Collection Center on January 2, 2025, and the preservation number of the pediococcus acidilactici is GDMCC No: 65706. The strain is resistant to acid and cholate, has a remarkable inhibiting effect on growth and reproduction of pathogenic escherichia coli and staphylococcus aureus, is safe and non-toxic, does not have pathogenicity, and provides beneficial strain resources and biological materials for development of corresponding microbial preparations. Therefore, the compound can be used for preparing a biocontrol preparation for inhibiting the growth of pathogenic bacteria, preparing animal feed and preparing a preparation for preventing, improving and / or treating gastrointestinal tract pathogenic bacteria infection, so that the compound has a wide application prospect.

The invention discloses a method for enhancing Candida albicans biofilm resistance of azole drugs by using a zinc ion chelating agent, through the combined action of the zinc ion chelating agent N, N, N ', N'-tetra (2-picolyl) ethylenediamine (TPEN) and fluconazole, the activity of the Candida albicans biofilm can be reduced, and the Candida albicans biofilm can be enhanced. The early-stage adhesion process, the medium-stage yeast-to-hypha conversion process and the late-stage maturation process formed by the candida albicans biological membrane are inhibited, the structure of the candida albicans biological membrane is destroyed, then the high drug resistance of the candida albicans biological membrane to fluconazole is reversed, and the pathogenicity of the fluconazole is reduced; the problems of high drug resistance and sustained infection caused by the formation of a candida albicans biofilm are hopefully solved, and a direction is provided for the treatment of clinical fungal infection and the research and development of drugs.

The invention discloses a psychrobacter haeli PN-Ta3 derived from turbot intestinal tracts and application of the psychrobacter haeli PN-Ta3, and belongs to the technical field of microorganisms. The psychrophilus haeli PN-Ta3 is separated from the intestinal tract of a healthy cultured turbot, and is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan, China, the preservation date is January 25, 2024, and the preservation number is CCTCC NO: M2024201. The psychrobacter nivalis PN-Ta3 disclosed by the invention has the beneficial effects that the psychrobacter nivalis PN-Ta3 has no pathogenicity to healthy turbots and has good colonization performance, and after the psychrobacter nivalis PN-Ta3 is prepared into freeze-dried bacterial powder and is mixed with and added to feed the turbots, not only can the growth and intestinal development of the cultured turbots be promoted, but also the intestinal microbial structure is optimized; the composition can also delay the morbidity and death of turbots caused by infection of aeromonas salmonicida BHAS-1.

The invention discloses an application of an sscle13g095080 gene and a protein coded by the sscle13g095080 gene in regulation and control of pathogenicity of sclerotinia sclerotiorum. The nucleotide sequence of the sscle13g095080 gene is as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The invention discovers that the sclerotinia sclerotiorum sscle13g095080 gene is related to the pathogenicity of sclerotinia sclerotiorum, and the pathogenicity of sclerotinia sclerotiorum can be obviously reduced by knocking out the sclerotinia sclerotiorum sscle13g095080 gene Therefore, the sscle13g095080 gene can be used as an effective target for preventing and treating the sclerotiniose, compounds capable of preventing gene expression and protein expression, modification and positioning of the gene can be screened, occurrence of the sclerotiniose can be effectively controlled, and development of novel bactericides is facilitated.

The invention belongs to the technical field of microorganisms, and relates to a riemerella anatipestifer phage and application thereof. The riemerella anatipestifer bacteriophage provided by the invention is RA-XHX1, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M 20241268, and is classified and named as the riemerella anatipestifer bacteriophage. The bacteriophage is double-stranded DNA (Deoxyribose Nucleic Acid) and myuroviridae, can form transparent plaques on a solid culture medium, has no halo ring around and is clear and regular in edge; the bacteriophage has a relatively good killing effect on riemerella anatipestifer in a space environment, particularly high-pathogenicity riemerella anatipestifer, and can realize a broad-spectrum antibacterial effect when being applied to preparation of medicines for preventing and treating riemerella anatipestifer infection.

The invention discloses trichoderma asperellum TS7-1 capable of efficiently degrading macrolide antibiotics and application of the trichoderma asperellum TS7-1, and belongs to the technical field of microorganisms. The preservation number of the trichoderma asperellum TS7-1 is CGMCC (China General Microbiological Culture Collection Center) No. 41372. The Trichoderma asperellum TS7-1 (CGMCC No.41372) provided by the invention has high-efficiency degradation capability on various macrolide antibiotics (erythromycin, roxithromycin and azithromycin), the removal rates of the Trichoderma asperellum TS7-1, roxithromycin and azithromycin in 36h all reach 85% or above, and the Trichoderma asperellum TS7-1 has high-concentration antibiotic tolerance, degradation rate and degradation spectrum obviously superior to those of other strains in the prior art. The trichoderma asperellum TS7-1 is directly separated from a natural soil body which is not polluted by antibiotics, has no animal and plant pathogenicity hidden danger, has no microbial toxicity to degradation products of macrolide antibiotics, avoids secondary pollution, and has excellent environmental safety.

The invention discloses an ADF protein from phytophthora as well as a coding gene and application thereof. According to the present invention, the PsADF protein provided by the present invention is the protein represented by sequences 2 and 5 in Phytophthora sojae (Phytophthora sojae); the coding gene is shown as a sequence 3 and a sequence 4. Tests prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, specifically, after the 64-site mutation of the key amino acid functional site of the protein, the sporangium abnormality and zoospore quantity of the phytophthora sojae are reduced, and the pathogenicity is reduced. The protein provided by the invention has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and provides a molecular target for research and development of novel bactericides in the future. The corresponding ADF inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora sojae, phytophthora capsici and phytophthora infestans. The protein provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed based on the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.