34 results about "Chimeric virus" patented technology

The invention discloses a chimeric PEDV (porcine epidemic diarrhea virus) infectious clone plasmid, a PEDV chimeric virus as well as a construction method and application thereof. According to the invention, a PEDV genome is used as a skeleton for the first time, the chimeric virus for expressing the extracellular domain of the TGEV S protein is successfully rescued, and it is found that the TCID50 of the chimeric virus for expressing the extracellular domain of the TGEV S protein can be increased by 6.2 times compared with that of the chimeric PEDV for expressing the TGEV S protein. The recombinant virus is used as an inactivated vaccine for immunizing mice, so that the immunogenicity of TGEV S protein can be greatly improved, a body is stimulated to generate a higher-level neutralizing antibody aiming at TGEV, and the titer of the neutralizing antibody aiming at TGEV is improved by 16.8 times. Therefore, more possibilities are provided for modification of viral genomes, a novel method for improving immunogenicity of the TGEV inactivated vaccine is invented, and a basis is provided for research and development of the TGEV vaccine.

The application discloses a PEDV chimeric virus attenuation identification method based on reverse genetic operation and application of screening potential attenuated vaccine, and the PEDV chimeric virus CHM2013-SP BJ , CHM2013- BJ , CHM2013-SP BJ -ORF3 CHM , CHM2013-SP BJ -E CHM , CHM2013-SP BJ -M CHM , CHM2013-SP BJ -N CHM and CHM2013-(S+ORF3) BJ are constructed and rescued, loss-of-function test and gain-of-function test are carried out, and the attenuated chimeric virus CHM2013-SP BJ -ORF3 CHM and CHM2013-SP BJ -M CHM are obtained, and it is disclosed that the S gene of the PEDV variant BJ2011C promotes virus pathogenicity in cooperation with the ORF3, E and M genes, and the ORF3 gene is the most critical.

The invention relates to a porcine reproductive and respiratory syndrome chimeric virus vaccine strain as well as a construction method and application thereof, and belongs to the technical field of biology. The porcine reproductive and respiratory syndrome chimeric virus provided by the invention is an RNA (Ribonucleic Acid) virus, and the nucleotide sequence of the RNA virus is SEQ ID NO: 1. The chimeric virus has better safety and effectiveness, can relieve lung injury caused by the porcine reproductive and respiratory syndrome virus and promote removal of viruses in piglets, can be used as a vaccine candidate strain of the porcine reproductive and respiratory syndrome virus, and is beneficial to prevention and control of the porcine reproductive and respiratory syndrome.

The invention discloses a recombinant virus vector, a vesicular stomatitis-yellow rice chimeric virus and application of the vesicular stomatitis-yellow rice chimeric virus. The recombinant virus vector is a recombinant vector containing an expression cassette of a vesicular stomatitis-yellow rice chimeric genome; the expression cassette comprises a T7 promoter, a coding sequence of a vesicular stomatitis virus N protein, a coding sequence of a vesicular stomatitis virus P protein, a coding sequence of a vesicular stomatitis virus M protein, a coding sequence of a yellow virus F protein and a coding sequence of a vesicular stomatitis virus L protein which are connected in sequence. The replication-deficient recombinant virus for chimeric expression of the LayV F protein is constructed on the basis of the VSV vector, the safety is good, the immunogenicity is good, a high-titer specific IgG antibody and a neutralizing antibody aiming at LayV can be induced to be generated through intramuscular or subcutaneous injection, a new technical choice is provided for preventing or blocking LayV infection, and the replication-deficient recombinant virus has important use and popularization values.

This invention belongs to the field of biomedical technology, specifically relating to a method for constructing a highly cell-adaptive PEDV GIIc recombinant chimeric virus. Specifically, this invention, based on the PEDV viral S protein determining PEDV's cell adaptability, innovatively elucidates that S2 is the key subunit determining its cell adaptability. On this basis, this invention proposes a scheme that can increase viral titer and thus reduce vaccine production costs when preparing candidate vaccine strains of circulating PEDV strains (especially GIIc). This involves retaining the S2 subunit of the highly cell-adaptive strain and replacing the S1 subunit of the highly cell-adaptive strain with the S1 subunit of the circulating strain, or replacing the S2 subunit of the circulating strain with the S2 subunit of the highly cell-adaptive strain. This scheme can both preserve the immunogenicity of the circulating strain and improve the efficiency of high-titer vaccine production.

The present invention relates to polynucleotides comprising a sequence of a live, infectious, attenuated Flavivirus wherein a nucleotide sequence encoding at least a part of a Filovirus glycoprotein is located at the intergenic region between the E and NS1 gene of said Flavivirus, such that a chimeric virus is expressed, characterised in that the encoded sequence C terminally of the E protein of said Flavivirus and N terminally of the signal peptide of the NS1 protein of said Flavivirus comprises in the following order: a further signal peptide of a Flavivirus NS1 protein, a filovirus glycoprotein wherein the N terminal signal peptide is absent, a TM domain of a flaviviral E protein.

Provided are a chimeric virus expressing a porcine reproductive and respiratory syndrome virus (PRRSV)-derived peptide and a use thereof as a vaccine. The chimeric virus has an excellent immune stimulating effect and is useful as a vaccine that can effectively protect against PRRSV by suppressing viral amplification in target cells.

The present invention relates to a multivalent HPV immunogenic composition for preventing human papillomavirus (HPV) related diseases or infections and uses thereof. Said multivalent HPV immunogenic composition comprises: HPV virus-like particles assembled from L1 proteins of HPV Types 6, 11, 16, 18, 31, 33, 45, 52, and 58; and one or more HPV virus-like particles assembled from L1 proteins of other pathogenic HPV types. In one embodiment, said one or more other pathogenic HPV types are selected from HPV Types 35, 39, 51, 56 and 59. In one embodiment, at least one of said HPV virus-like particles is a chimeric HPV virus-like particle, and said chimeric HPV virus-like particle comprises one or more chimeric HPV L1 proteins.

The invention belongs to the technical field of genetic engineering, and particularly relates to a chimeric virus-like particle vaccine of feline coronavirus and feline panleucopenia virus and application of the chimeric virus-like particle vaccine. The invention provides chimeric virus-like particles of feline coronavirus and feline panleucopenia virus. The chimeric virus-like particles contain feline coronavirus type I S protein and feline panleucopenia virus VP2 protein. The invention also provides a preparation method and application of the chimeric virus-like particle. After the vaccine prepared from the chimeric virus-like particles is used for immunizing mice and cats, a high-titer antibody level can be generated, and the organism is stimulated to generate cellular immunity; the immunized cat can effectively resist invasion of cat coronavirus and cat panleucopenia virus. The chimeric virus-like particle has good immunogenicity and can be used for preventing and controlling cat infectious diseases.

The application discloses a chimeric Kunjin virus of tamabi virus prM-E and a preparation method and application thereof. On the basis of a reverse genetic manipulation system of the Kunjin virus KUNV, the KUNV prM / E gene is replaced by the DTMUV prM / E gene to construct a chimeric DTMUV virus infectious clone plasmid with the KUNV as a skeleton, which is named as pUC19-KUNV-DTMUV prM / E. Then, the chimeric tamabi virus prM-E Kunjin virus is obtained through virus rescue, and is named as rKUNV-CHv-prM / E (CHv). Analysis on the virus characteristics shows that the CHv can effectively proliferate in BHK-21 and DEF cells, and the duck embryo virulence is weaker than that of the KUNV and the DTMUV. The animal experiment results show that the CHv only detects obvious virus levels in the brain, and does not produce viremia; the CHv after immunization can produce similar IgG antibody levels to those after immunization of the KUNV and the DTMUV. Therefore, the recombinant virus of the chimeric DTMUV prM / E with the KUNV as a skeleton prepared by the application can induce the duckling to produce sufficient immune protective neutralizing antibody levels, and can be applied to preparation of a duck tamabi virus vaccine.

The present specification relates to a chimeric virus expressing an avian influenza antigen and a vaccine composition comprising same.

The application discloses a bovine viral diarrhea virus chimeric virus expressing porcine circovirus type 3 capsid protein and a preparation method thereof. In the experiment, a gene sequence for encoding a porcine circovirus type 3 capsid protein and a His tag fused with the C terminal of the protein is inserted into different sites of a bovine viral diarrhea virus genome through a circular polymerase extension reaction, and the chimeric virus is prepared through virus rescue of the obtained circular DNA. The His tag sequence in the gene sequence and the bovine viral diarrhea virus genome are connected through a 2A peptide sequence, so as to realize the target of effectively releasing the antigen through the co-expression of the heterologous protein. The chimeric virus prepared by the application retains the infectivity and replication capacity of the parent virus, can stably replicate in cells and efficiently express the target antigen, and provides an efficient and flexible virus carrier platform for the research and development of a porcine circovirus type 3 vaccine.

The compositions and methods are described for generating an immune response to an antigen. The compositions and methods described herein relate to a modified vaccinia Ankara (MVA) vector encoding one or more viral antigens as a fusion product with a viral glycoprotein and matrix protein for generating a protective immune response to a subject to which the vector is administered. The compositions and methods of the present invention are useful both prophylactically and therapeutically and may be used to prevent and / or treat diseases.

The invention relates to the field of bioengineering, and discloses a DNA molecule for coding a chimeric virus as well as a preparation method and application of the DNA molecule. The DNA molecule contains a 5'non-coding region sequence, a coding sequence of capsid protein C, a coding sequence of prM-E protein, a coding sequence of non-structural protein NS and a 3 'non-coding region sequence which are connected in series; wherein the coding sequence of the prM-E protein is derived from Uu figure virus, and the 5'non-coding region sequence, the coding sequence of the capsid protein C, the coding sequence of the non-structural protein NS and the 3 'non-coding region sequence are derived from encephalitis B virus. The DNA molecule and / or the chimeric virus have / has a good application prospect in the aspects of preventing Uusu virus infection and promoting secretion of Th1 cell factors.

A composition includes a contraceptive chimeric virus-like particle with an antigenic carrier domain and one or more antigenic regions from a sperm cell in the antigenic carrier domain, with the antigenic carrier domain including human papillomavirus L1 capsid protein and the antigenic regions including one or more structural elements of the Catsper ion channel complex. When administered to a patient, the contraceptive vaccine stimulates production of anti-sperm antibodies that, upon binding to a sperm cell, inhibit the sperm cell's motility and thus inhibit the ability of the sperm cell to fertilize an egg cell. The induced immunoinfertility of the composition can be reversed for brief or extended lengths of time by overdosing the patient with a reversal agent lacking the antigenic carrier domain but having a protein sequence substantially identical to that of the one or more antigenic regions to sequester the anti-sperm antibodies.

Disclosed herein are compositions and methods for the treatment of cancer in a human subject comprising administration of a recombinant chimeric vector. The recombinant chimeric vector comprise a cancer-specific CD8+ T cell epitope which may induce an inflating memory CD8+ and / or CD4+ T cell response in the subject.

The present disclosure relates to a recombinant chimeric virus-like particle (cVLP) vaccine composition against rotavirus infection. The vaccine comprises a virus-like particle fragment including a hepatitis B core (HBc) protein functioning as an adjuvant, into which a heterologous immunogenic domain derived from the rotavirus VPS* protein is inserted within the major immunodominant region (MIR) of HBc. The construct is encoded by SEQ ID NO. 1, and expressed in a prokaryotic system. The cVLPVPS* vaccine composition of the present disclosure offers a non-replicating, protein-based alternative to live attenuated vaccines, and the platform may help address challenges related to safety, strain specificity, and antigen delivery.

The invention belongs to the technical field of biological medicine, and particularly relates to a VSV-WEEV recombinant virus, a composition containing the VSV-WEEV recombinant virus, a nucleic acid molecule and application. The VSV-WEEV recombinant virus is constructed by replacing a vesicular stomatitis virus G protein with a western equine encephalitis virus structural protein E3E2E1. The recombinant virus is identified and subjected to immunogenicity evaluation, serum of a mouse immunized by the recombinant virus shows high antibody titer aiming at the WEEV, and a plaque reduction neutralization experiment also proves that the serum can effectively inhibit cell infection caused by another chimeric virus, namely SINV-WEEV, replacing a WEEV wild strain.

The invention provides a recombinant protein, a biological material, an avian influenza and Newcastle disease chimeric virus-like particle, a vaccine and a preparation method and application thereof, the recombinant protein comprises an SOC protein, a Newcastle disease virus HN protein and an avian influenza virus HA protein, the amino acid sequence of the recombinant protein is shown as SEQ ID NO.1, the amino acid sequence of the SOC protein is shown as SEQ ID NO.2, the amino acid sequence of the Newcastle disease virus HN protein is shown as SEQ ID NO.1, and the amino acid sequence of the Newcastle disease virus HN protein is shown as SEQ ID NO.2. The amino acid sequence of the Newcastle disease virus HN protein is as shown in SEQ ID NO.3, and the amino acid sequence of the avian influenza virus HA protein is as shown in SEQ ID NO.4. The avian influenza and Newcastle disease chimeric virus-like particle is formed by assembling the recombinant protein in vitro. Compared with active avian influenza virus and Newcastle disease virus, the virus-like particle prepared by the invention can induce effective cell and humoral immune response under the condition of no adjuvant, has strong immunogenicity, does not have the risk of incomplete inactivation or virulence recovery, and can be prepared into a safe, stable, multivalent and efficient bivalent subunit vaccine.

The present disclosure relates to a chimeric virus expressing an infectious bursal disease antigen and a vaccine composition comprising same.

This disclosure describes a chimeric respiratory syncytial virus encoding a chimeric RSV and hMPV F protein and uses of the chimeric virus or components therein in a vaccine. In certain embodiments, this disclosure describes a live attenuated vaccine comprising an RSV backbone substituting the F proteins of RSV, for a chimeric RSV and hMPV F protein.

Chimeric viruses having a vesicular stomatitis virus (VSV) background where the VSV G protein is supplemented or replaced with an alphavirus glycoprotein(s), or a functional fragment(s) thereof, are provided. A preferred alphavirus is Chikungunya virus. In particular embodiments, the glycoprotein(s) is or includes E3, E2, K6, and E1 proteins of an alphavirus, preferably Chikungunya virus. Methods of using the chimeric viruses for treatment of cancers, particularly brain cancers and metastasis thereof are also provided. In some embodiments, the chimeric viruses retain superior oncolytic activity to infect and destroy cancer cells selectively, such as glioblastoma and intracranial melanoma metastases. In some embodiments, the chimeric viruses have reduced toxicity to e.g., heathy cells relative to a control such as the parent VSV with the VSV G protein.

Provided herein are live attenuated viruses for protection against respiratory syncytial virus (RSV) and / or coronavirus Sars-CoV-2. The live attenuated chimeric virus strains utilize a master backbone based on a live attenuated influenza virus (LAIV), which includes a deletion of the viral virulence element, the NS1 (non-structural protein 1) (DeLNS1). These chimeric strains are engineered to express one or more antigens of RSV alone or in combination with Sars-CoV-2. The chimeric virus strain can protect a subject in need thereof against a challenge from any of RSV, Sars-CoV-2, influenza, or a combination thereof. This viral vector system offers an important strategy for developing highly attenuated and immunogenic live attenuated vaccines with the capacity to induce protective immunity against the three respiratory infections.

The present disclosure relates to a recombinant chimeric virus-like particle (cVLP) vaccine composition against rotavirus infection. The vaccine comprises a virus-like particle fragment including a hepatitis B core (HBc) protein functioning as an adjuvant, into which a heterologous immunogenic domain derived from the rotavirus VPS* protein is inserted within the major immunodominant region (MIR) of HBc. The construct is encoded by SEQ ID NO. 1, and expressed in a prokaryotic system. The cVLPVPS* vaccine composition of the present disclosure offers a non-replicating, protein-based alternative to live attenuated vaccines, and the platform may help address challenges related to safety, strain specificity, and antigen delivery.

The invention discloses a Kujin virus embedded with tembusu virus prM-E as well as a preparation method and application of the Kujin virus. According to the invention, on the basis of a reverse genetic operating system of a KUNV (Kumphu virus), a DTMUV prM / E gene is used for replacing a KUNVprM / E gene, and a chimeric DTMUV virus infectious clone plasmid taking the KUNV as a skeleton is constructed, and is named as pUC19-KUNV-DTMUV prM / E. Then, virus rescue is carried out, so that the kumpu virus embedded with the tembusu virus prM-E is obtained, and the kumpu virus is named as rKUNV-CHv-prM / E (CHv). The analysis of virus characteristics shows that CHv can effectively proliferate in BHK-21 and DEF cells, and the virulence of duck embryos is weaker than that of KUNV and DTMUV. Animal experiment results show that the CHv only detects an obvious virus level in the brain and does not generate viremia; after CHv immunization, an IgG antibody level similar to that after KUNV and DTMUV immunization can be generated. Therefore, the recombinant virus which takes the KUNV as the skeleton and is embedded with the DTMUVprM / E can induce the duckling to generate a neutralizing antibody level with enough immune protection, and can be applied to preparation of a duck tembusu virus vaccine.

The present invention relates to polynucleotides comprising a sequence of a live, infectious, attenuated Flavivirus wherein a nucleotide sequence encoding at least a part of a arenavirus glycoprotein protein is located at the intergenic region between the E and NS1 gene of said Flavivirus, such that a chimeric virus is expressed, characterised in that the encoded sequence C terminally of the E protein of said Flavivirus and N terminally of the signal peptide of the NS1 protein of said Flavivirus comprises in the following order: —a further signal peptide of a Flavivirus NS1 protein, —an arenavirus Glycoprotein protein lacking the N terminal signal sequence and the GP2 transmembrane domain, —a TM1 and TM2 domain of a flaviviral E protein.

The invention relates to the technical field of biological medicines, in particular to an optimized SARS-CoV-2 chimeric virus-like particle based on an influenza virus skeleton and an application of the optimized SARS-CoV-2 chimeric virus-like particle. The amino acid sequence of the spike protein of the virus-like particle comprises an extracellular domain amino acid sequence; the amino acid sequence of the extracellular domain has S-6P mutation (F817P, A892P, A899P, A942P, K986P and V987P) and Frelin restriction enzyme cutting site substitution (RRAR-GSAS). The optimization mode can maintain natural trimer conformation before S protein fusion and effectively improve the stability of the S protein, so that the dissociation phenomenon of the protein is effectively improved, and the influence of the dissociation phenomenon on effective epitopes is avoided. In order to improve the secretory expression efficiency of the VLPs, the protein expression is optimized by using a signal peptide derived from an insect cell-baculovirus expression system. The immunogenicity and immune protection efficacy of the vaccine can be effectively improved.

The invention relates to a vaccine, in particular to a chimeric virus-like particle vaccine based on an echovirus 30 type key epitope as well as a preparation method and application of the chimeric virus-like particle vaccine. The invention provides a method for screening antigen epitopes, which comprises the following steps: firstly, predicting the antigen epitope of capsid protein VP1 through bioinformatics analysis, and then screening epitopes which can be effectively combined with anti-VP1 complete sequence serum through enzyme-linked immunosorbent assay. Results of an enzyme-linked immunosorbent assay show that the expressed antigen epitope peptide E30-6 (SEQ ID NO: 2) can be strongly combined with E30 VP1 antiserum, so that one high-immunogenicity antigen epitope peptide is screened based on the complete sequence of the echovirus 30 VP1, which has important guiding significance for research and development of echovirus 30 vaccines. Besides, the HBc-E30-6 vaccine is designed and prepared based on the epitope, the HBc-E30-6 vaccine is high in purity and can be self-assembled into VLP nanoparticles, and more importantly, serum immunized by the vaccine can effectively neutralize the echovirus 30.

The present application relates to the technical field of veterinary biological products, and particularly relates to a chimeric recombinant plasmid pPRRSV-VP2-Y, a chimeric virus thereof and application thereof. The present application inserts a VP2-Y gene derived from porcine parvovirus between ORF1b and ORF2a of a high pathogenicity PRRSV cell-attenuated strain HuN4-F112 to construct a chimeric recombinant plasmid pPRRSV-VP2-Y; the virus rescued after transfecting MARC-145 cells with the chimeric recombinant plasmid constructed by the present application has similar biological characteristics to a parent virus vHuN4-F112, and meanwhile, the chimeric recombinant vaccine strain constructed based on the above-mentioned recombinant plasmid has good safety to pigs, can effectively induce an immune response of the body after immunization, can produce a high level of PRRSV N protein antibodies similar to the parent vaccine, and can induce PPV VP2 antibodies.

Disclosed herein are compositions and methods for the treatment of cancer in a human subject comprising administration of a recombinant chimeric vector. The recombinant chimeric vector comprise a cancer-specific CD8+ T cell epitope which may induce an inflating memory CD8+ and / or CD4+ T cell response in the subject.