7 results about "Vaccine virus" patented technology

The invention relates to the fields of molecular biology and virology, and provides a method for preparing a specific MHC haplotype-derived cell-binding virus. The method comprises the following steps: firstly, obtaining chick embryos of different haplotypes, separating CEF cells, transiently expressing exogenous fluorescent protein through electrotransfection, then carrying out virus inoculation, carrying out flow sorting to obtain a fluorescent positive cell population carrying MDV, and carrying out quantitative analysis on the cell population to obtain the specific MHC haplotype chicken Marek's Disease Virus (MDV), thereby obtaining the specific MHC haplotype chicken Marek's Disease Virus Marek's Disease Virus Marek's Disease Virus Marek's Disease Virus. And carrying out in-vitro propagation to obtain the purified MDV with a specific haplotype source. The purified virus can provide a test material for related research of haplotype on virus infection, and can be widely applied to in-vitro purification of different cell binding viruses and related research of specific MHC molecule presenting virus peptides. The method disclosed by the invention is beneficial to carrying out related research aiming at the host immune response of the virus and analyzing the hereditary basis of haplotype to MDV differential resistance, and is expected to be applied to preparation of vaccine virus.

The invention discloses a bovine virus diarrhea live vaccine based on suspension culture and a preparation method thereof, and relates to the field of animal vaccines. The bovine viral diarrhea virus vaccine is a freeze-dried vaccine, and each vaccine contains a bovine viral diarrhea virus BVDV attenuated strain antigen and a freeze-drying protective agent; wherein the virus content of the antigen of the BVDV attenuated strain is 105-107 CCID50 / piece, and the BVDV attenuated strain is a cytopathic effect type attenuated strain suitable for suspension culture; the freeze-drying protective agent comprises trehalose, cane sugar, gelatin hydrolysate, sodium glutamate and polyvinylpyrrolidone. The suspension culture technology is adopted, large-scale, efficient and stable production of BVDV antigens is achieved, an optimized freeze-drying formula is combined, the obtained vaccine is high in virus titer, good in immunogenicity, high in stability, good in safety, advanced in production process, controllable in cost and suitable for industrial large-scale production.

This invention discloses PCR primers and probes for detecting ILT live vaccine virus and wild-type virus. The primers include a universal upstream primer: 5'-GCACAAAGTCCAAATCTTCT-3' and a universal downstream primer: 5'-TCAATCACAGACGGTACTTTC-3'. The probes include a vaccine virus-specific probe: 5'-FAM-CAAGTTGATGAATATGCTCAC-MGB-3' and a wild-type virus-specific probe: 5'-ROX-CAAGTTGATGAATGTGCTCA-MGB-3'. The 5' end of the vaccine virus probe is labeled with the FAM fluorescent group, and the 5' end of the wild-type virus probe is labeled with the ROX fluorescent group. The 3' ends of both probes are labeled with the MGB quencher group. Using these primers and probes, ILT live vaccine virus and wild-type virus can be detected and distinguished rapidly and accurately, and can be used for epidemic monitoring in farms.

The application provides a feline calicivirus LZ-2016 strain and a vaccine composition. The feline calicivirus LZ-2016 strain has a high virus titer, the strain has strong pathogenicity to cats, and can cause typical symptoms such as oral ulcer and conjunctivitis. The inactivated product of the strain can produce a good immune response when cats are immunized, has good immunogenicity, can better stimulate the body to produce high-level neutralizing antibodies against FCV, can provide high immunoprotection rate to FCV attack, and has basic potential for developing a vaccine. The strain can be prepared into a vaccine and used for feline calicivirus prevention and treatment, provides an important vaccine virus source for feline calicivirus prevention and control in China, and realizes effective feline calicivirus prevention and control.

The invention relates to the field of virology. In particular, the invention relates to a polypeptide for quantitatively detecting the HA protein of H5N1, a kit containing the polypeptide, and a method for quantitatively detecting the HA protein of H5N1 by using the polypeptide. The method is a quantitative method with high throughput, high selectivity and high sensitivity, and can be used for absolute quantification of HA protein in H5N1 vaccine virus liquid.

The application discloses a PCR primer and probe for identifying serum type I Marek's vaccine virus and wild virus, wherein the primer comprises an upstream primer with a sequence as shown in SEQ ID No:1 and a downstream primer with a sequence as shown in SEQ ID No:2; and the probe comprises a wild virus-specific probe with a sequence as shown in SEQ ID No:3 and a vaccine virus-specific probe with a sequence as shown in SEQ ID No:4. Through the primer and the probe, the serum type I Marek's vaccine virus and the wild virus can be efficiently, conveniently and quickly identified, so that early warning, accurate prevention and control and purification of Marek's disease are realized.

The invention discloses a freeze-drying protective agent of an infectious laryngotracheitis recombinant fowlpox virus genetic engineering vaccine as well as a preparation method and application of the freeze-drying protective agent, and belongs to the technical field of veterinary biological products. In order to reduce the virus loss of the avian infectious laryngotracheitis recombinant fowlpox virus genetic engineering vaccine in the freeze-drying process and improve the virus titer, different freeze-drying protective agent formulas and freeze-drying curves are screened, and a freeze-drying protective agent capable of reducing the virus loss of the vaccine and a matched freeze-drying process of the freeze-drying protective agent are successfully developed. The screened freeze-drying protective agent and freeze-drying curve can reduce the virus loss of the avian infectious laryngotracheitis recombinant fowlpox virus genetic engineering vaccine in the freeze-drying process, greatly improve the feather quantity contained in each bottle of vaccine, and ensure the stability of the vaccine during the vaccine storage period; the production cost can be reduced while the vaccine quality is improved, and actual production requirements are met.