121 results about "Immune state" patented technology

The invention relates to an immune gene prognosis model for predicting hepatocellular carcinoma tumor immune infiltration and postoperative survival time, and belongs to the technical field of biological medicines. The model can be used for evaluating the infiltration degree of immune cells in a tumor in clinical practice by detecting the expression levels of 22 specific immune related genes of ahepatocellular carcinoma patient, so that the model can be used for predicting hepatocellular carcinoma tumor immune infiltration in clinical practice and improve the prediction capability of the liver cancer immunotherapy response. The model can be used for judging the postoperative overall survival risk of a patient and guiding the formulation of a postoperative treatment strategy, and the corresponding microarray chip kit can realize the standardization and convenience of detection. Meanwhile, the immune gene prognosis model provided by the invention can increase the prediction accuracy andthe clinical net income of a hepatocellular carcinoma TNM staging system on the total survival time of three years and five years after operation. As a molecular marker for objectively and accuratelyevaluating the tumor immune state and poor prognosis risk of hepatocellular carcinoma, the model can realize accurate implementation of hepatocellular carcinoma immunotherapy and accurate prognosis prediction.

The invention discloses a preparing method and application of a human mesenchymal stem cell-sourced exosome beautifying preparation. The beautifying preparation comprises 92-108 parts of human umbilical cord mesenchymal stem cell exosome, 8-13 parts of mannitol, 0.42-0.55 part of oligopeptide-32, 1.3-2.5 parts of tetrapeptide-4, 0.7-1.3 parts of palmitoyl tripeptide-1 and 0.8-1.2 parts of palmitoyl pentapeptide-4. The preparation method includes: evenly mixing and stirring the human mesenchymal stem cell exosome, the mannitol, the oligopeptide-32, the tetrapeptide-4, the palmitoyl tripeptide-1and the palmitoyl pentapeptide-4 according to the weight, filtering through a filter membrane, performing split charging, and performing freeze drying to obtain the human mesenchymal stem cell-sourced exosome beautifying preparation. The exosome beautifying preparation can be externally applied to the epidermal layer or guided into an epidermis deep layer and the corium layer through a microneedle, a rolling needle or a hydro-lifting needle. The ImmuReg technology is utilized to bring the optimal repairing effect of freeze-dried powder into play, the skin immune state can be precisely adjusted, and accordingly aged cells can be removed, and skin cells can be rejuvenated.

The invention discloses a network virus transmission behavior modeling method. The method comprises a network initialization process, a virus transmission process, a node detection process, an immunization and immunization failure process, a model evaluation process and an immunization strategy proposing process. When a model runs, an infected state (I) node transmits a virus to a neighbor node at certain probability; an infectious state (S) node is infected into the infected state (I) node by the virus at the certain probability; meanwhile, the infected state (I) node is isolated at the certain probability, and becomes an isolated state (Q) node which does not have the virus transmission capacity and cannot be infected; and an immune state (R) node which cannot be infected by the virus appears in the model through treatment on the isolated state (Q) node and immunization on all nodes. The factors affecting virus transmission in the SIOR model include total node number n, average node degree <k>, initially infected node number n<0>, virus transmission rate alpha, isolating treatment rate beta and isolating recovery rate lambda; and the cured node has immune efficacy probability mu, vaccine injection rate gamma and vaccine failure rate eta. An achievable immunization strategy is proposed on the basis of these factors.

The invention discloses a technology in the aspect of immunology, in particular to a method for amplifying natural killer (NK) cells and CD8 lymphocyte by interleukin 2 and interleukin 15 receptor (IgG1 Fc) and interleukin 2 complex. A polypeptide complex is cultured by an interleukin 2 and the interleukin 15 receptor which are protein having the expression function, and the aim of changing the immune state is achieved by the lymphocyte or a lymphocyte precursor cell activated and amplified by the polypeptide complex. The patient is helped to resist a tumour, viruses and bacteria by improving the immunity through the interleukin 15 receptor (IgG1 Fc) and interleukin 2 complex, and the realizing effect is good. The invention has wide prospect in the aspect of immunology.

The invention discloses dendrobium candidum compound chewable tablets which are characterized by being prepared from the following raw materials in parts by weight: 30-50 parts of dendrobium candidum extract, 5-10 parts of lucid ganoderma, 5-15 parts of fructus lycii, 20-30 parts of sweetening agent, 3-5 parts of flavoring agent, 20-30 parts of hydroxypropyl methyl cellulose, 5-15 parts of maltodextrin and 1-3 parts of lubricant. The invention also discloses a preparation method of the chewable tablets. After the dendrobium candidum compound chewable tablets disclosed by the invention are taken, the functional balance of the internal organs of a human body can be adjusted, the immunity of the human body is improved, the immune state of the human body is effectively and bidirectionally adjusted, and the internal organs are strong and vigorous to prevent disease factors and harmful germs from invading the human body.

The invention discloses T cell subsets in lung cancer and feature genes thereof. A single-cell transcriptome analysis technology is adopted, and by analyzing a single-cell gene expression profile of infiltrating T cells in the lung cancer tissue, the T cell subsets capable of reflecting body tumor immune states of patients with the lung cancer are separated and characterized, namely the exhaustedCD 8+ T cells or regulatory T cells capable of expressing genes including TNFRSF9, TNFRSF18 and LAYN are separated and characterized. Through research, relations between lung cancer prognosis and thenew feature genes which include TNFRSF9, TNFRSF18 and LAYN and are expressed by the cell subsets are further determined. Therefore, the T cell subsets in lung cancer and the feature genes thereof canbe used for diagnosis and monitoring of lung cancer prognosis and serve as new targets for lung cancer immunotherapy.

The invention provides a method for detecting canine rabies virus antibody (IgG) and a detection kit. The kit is composed of a coated plate and a reagent reaction system, and comprises a rabies virus antigen-coated reaction plate, a standard substance, positive contrast serum, a washing lotion (20X), a sample weak solution, an enzyme-marked combination substance, a developer A, a developer B and a stopping solution. The method is characterized in that the method uses an ELISA method to determine that the content of the canine rabies virus antibody (IgG) reaches a absorbance corresponding to a protective level by detecting the standard substance of the kit, and by compared the absorbance of the sample to be detected with the absorbance of the standard substance, the content of the canine rabies virus antibody (IgG) contained in the sample to be detected is judged whether to reach an immunization protective level. The method and the kit can simultaneously detect a lot of samples, and a detection result is remarkably with a result by a neutralization experiment, has high accuracy degree, is suitable for monitoring an immunization inoculation effect and determining an individual immunization state, and can be used for investigating animal eqpidemic diseases.

The invention discloses porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine which is prepared by mixing inactivated porcine parvovirus virus solution and nanometer alumina gel adjuvant according to the ratio of 1 to 2. According to the invention, safety evaluation to the vaccine is performed on suckling mice and pigs, the results show that after immunization, one suckling mouse dies, no sensitization response is caused on a baby pig, as the same, no obvious full body or partial reaction can be observed, so that the inactivated vaccine is fully proved to be safe and reliable. The porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine set has a CD4 +/CD 8 + relative value in the fourth immunization week, which is obviously higher than that of other vaccine set, the relative value is increased to 2.97 from 2.11, which shows that the vaccine enhances the immune state of cells of mice. The porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine provided by the invention can induce the secretory expression of IFN-Gamma, and the vaccine can remarkably enhance the immune response reaction produced by Th 1 type cells, to induce the Th 1 type cells to secrete IFN- Gamma. Therefore, the immune level of cells can be increased, and a stronger cellular immunity response can be produced.

The invention relates to a detection system for detecting a human immune state and belongs to the technical field of gene detection. The detection system provided by the invention is used for detecting 20 genes, including IFI27, ISG15, IFI44L, RSAD2, HERC5, IFITM3, IFI44, IFIT3, EPSTI1, IFIT1, HP, ANXA3, ARG1, FCGR1B, S100A12, MMP9, IL18R1, TLR3, GYG1 AND FCGR1A in peripheral blood; the assessment for the human immune state can be realized in the manner of increasing or reducing the expression level of each gene; and the genes are utilized to guide accurate medical treatment, including disease accurate parting, prognosis, treatment tracking and therapeutic evaluation. The detection system has excellent clinic application values and wide application prospects.

The invention discloses a method for quantifying a tumor immune state based on radiomics, wherein the method comprises the following steps: S1, collecting clinical information, immunohistochemical characteristics, overall survival OS and disease-free survival DFS of tumor patients; S2, obtaining a CT image sample group of solid tumors; S3, randomly dividing the patients into a training set and a test set in proportion; S4, calculating the immunoscore by an immune marker, and dividing the patients into a set with high immune state and a set with low immune state; S5, segmenting an interest region, and extracting radiomics characteristics of the solid tumors in the interest region; S6, screening the radiomics characteristics related to the immune state from the radiomics characteristics in the training set, and training a prediction model; and S7, predicting the image immunoscore of the tumor patients, obtaining the image immune state, and then predicting the DFS and the OS in the patients. The immune state of the solid tumors such as colorectal cancer, lung cancer, gastric cancer and breast cancer is predicted by the CT image characteristics, and the analysis of pathological sections and immunohistochemistry is reduced or avoided.

The invention provides a construction method for an applicability evaluation model of an immune state of T lymphocyte of hepatic diseases of hepatitis B. The method comprises the following steps of (1) detecting T cell activator factors and inhibitory/exhaustion molecules of peripheral blood of a patient through a flow cytometry in order to obtain levels of the T cell factors of each detection sample; (2) collecting population statistics, virology, liver function and liver hardness value data of each case and carrying out correlation analysis on clinical indexes and a T cell immunity evaluation index to find the clinical indexes having the close correlation; (3) utilizing the different clinical indexes to establish a plurality of scored ROC curve models, comparing areas under the ROC curve, flexibilities and specificities of different models and selecting the optimal ROC curve; and (4) obtaining different immuno-clinic score equations according to the optimal ROC curve in order to further optimize the optimal immuno-clinic score equation used for evaluating the overall immune state of the T cell.

The invention relates to an application of PD-L1 gene modified bone marrow mesenchymal stem cells in graft rejection. According to the invention, immune state can be regulated and graft rejection can be inhibited, significant guidance in clinical application of xenogeneic organ transplantation is provided, foundation for inducing peripheral immune tolerance is established, and application prospect is broad.

A method of obtaining a high yield of differentiated human cells and organs includes the steps of providing typed human bone marrow or cord blood stem cells, providing pre-immune non-human mammalian fetuses, implanting the cells into the fetuses, permitting the fetuses to grow for a sufficient time to produce differentiated cells in hybrid organs, and harvesting the differentiated cells from the mammals. A method is presented to produce hybrid functioning human-animal solid organs for clinical transplantations. The method includes obtaining bone marrow mononuclear cells (BMNC) from the patient, obtaining enriched populations of HSC from the BMNC, and transplanting the enriched cells into Preimmune fetal sheep or pigs intraperitoneally to produce functioning donor (patient)-animal hybrid organs. A method is also presented in which enriched HSC isolated from pre-HLA typed normal human fetal liver/bone marrow, cord blood, or bone marrow will be transplanted into preimmune fetal sheep or pigs in order to create functioning human-animal hybrid organs that can be transplanted into compatible patients. Methods are also presented to obtain high yield of different types (e.g. hepatocytes) of donor (patient or HLA-typed normal donors) cells from the human-animal hybrid organs that can be used either for transplant into patients and/or treatment of the patient. Also disclosed is a method of producing purified human proteins that includes providing a non-human, pre-immune mammal into which human bone marrow or cord blood cells has been implanted into the mammal at the pre-immune state, obtaining blood from the non-human mammal, and isolating the human proteins from the mammalian blood.

The invention relates to a preparation method for a monoclonal antibody specifically binding vardenafil and an analog thereof. The preparation method comprises the steps of: synthesizing artificial immunizing antigens and artificial testing antigens, screening mice under immune state and the monoclonal antibody, and conducting structural specificity identification of the monoclonal antibody, specifically, mixing M-BSA with the vardenafil, regulating pH, adding a glutaraldehyde coupling agent, slowly stirring with a magnetic stirrer for reaction, dialyzing with distilled water, freezing and drying under vacuum condition, and synthesizing the artificial immunizing antigens; mixing M-OVA with the vardenafil, regulating pH, adding a glutaraldehyde coupling agent, slowly stirring with a magnetic stirrer for reaction, dialyzing with distilled water, freezing and drying under vacuum condition, and synthesizing the artificial testing antigens; and using a competitive inhibition ELISA method to detect the coasensual reaction between the monoclonal antibody and the vardenafil and piperidenafil and identifying the monoclonal antibody which has high coasensual reaction rate and can specifically bind the vardenafil and the analog thereof. The affinity equilibrium constant of the monoclonal antibody prepared by the method on the vardenafil and the analog thereof is higher than 10M.

The invention discloses a computer virus spreading source tracing method based on a complex network, and relates to the technical field of computer virus network spreading. The source tracing method comprises the following steps: (1) building a computer virus spreading network diagram; (2) building a virus infection state vector; (3) building an immune state vector; and (4) building a network time sequence state matrix. Through adoption of the computer virus spreading source tracing method based on the complex network, an effective strategy is provided for the control of computer virus spreading; a computer virus defense system is further enriched; and the security of a computer is improved.

The invention discloses a method and a system for selecting an individualized tumor neoantigen based on an expected curative effect. The method comprises the following steps: establishing and optimizing a cell selectivity model; establishing and optimizing a treatment effect model, carrying out auxiliary selection on individualized tumor neoantigens and the like, and can maximize the utilization of biological experiments to carry out trial and error, establish the most accurate cell selectivity model and maximize the utilization of the biological experiments to improve the effect on individual patients under the condition of not involving the patients; the method further considers the correlation between the selectivity of new antigen cells and the immune state of patients, builds a complete curative effect prediction model, evaluates the applicability of a specific new antigen to a specific patient according to an actual curative effect, provides high-value reference information for a doctor to select an appropriate new antigen for immunotherapy, utilizes the actual effect feedback of doctor treatment to the maximum extent, and continuously improves the prediction precision of the overall effectiveness of new antigen immunotherapy.

The invention provides application of salmonella pullorum secreted protein SpiC. The salmonella pullorum secreted protein SpiC can be taken as a biological marker for diagnosis when salmonella infects a host, and is used for preparing materials for pullorum disease immunodetection or materials for diagnosis. According to the invention, the protein is further utilized to establish materials applied to pullorum disease ELISA (enzyme-linked immuno sorbent assay) of a SpiC antibody generated in the host, and a pullorum disease ELISA method is correspondingly given. The protein-based ELISA method for detecting the SpiC antibody generated in the host is good in specificity and high in flexibility, and can be applied to the evaluation of organism immune states and the research relevant to infectious disease diagnosis.

The invention discloses a green immunopotentiator for pigs. The green immunopotentiator has various functions of prevention, healthcare, treatment, immunoregulation and the like. The green immunopotentiator for the pigs is an ideal, green, environment-friendly, safe and novel feed additive replacing an antibiotic drug. Compound Chinese herbal medicines such as houttuynia cordata, kudzu vine roots, gynostemma pentaphylla, isatis roots, glossy privet fruits, enteromorpha, liquorice, radix scutellariae, acanthopanax, folium isatidis, Chuanxiong rhizome, honeysuckle and stevia are subjected to 'Chinese herbal medicine ultrasonic wave-assisted extraction, multi-strain aerobic fermentation and anaerobic fermentation combined two-step fermentation' to obtain the fermented Chinese herbal medicine feed additive. The fermented Chinese herbal medicine feed additive has double advantages of the Chinese herbal medicines and probiotics, health of pig intestinal tracts can be efficiently conditioned, both principal and secondary aspects of diseases are treated, vital essence is reinforced, primordial qi is strengthened, intestinal micro-ecological balance and optimal immune state are kept, the digestion and absorption rate of nutrient substances is increased, pig body immunity is enhanced, raising income is increased, raising risk is reduced, and the feed additive replaces antibiotics, has no negative effect on the pigs, avoids the problems of drug residues, drug resistance and the like, and has a wide market prospect.

The invention discloses a preparation method and application of a Chinese herbal immunostimulant for flatfish. The fermented Chinese herbal immunostimulant for the flatfish has multiple effects of prevention, health, treatment, immune regulation and the like, and is an ideal, green, environment-friendly, safe and novel feed additive for substituting antibiotics. The fermented Chinese herbal feed additive is prepared from hericium erinaceus, artemisia capillaris, codonopsis pilosula, honeysuckle, poria cocos, selfheal, liquorice, astragalus membranaceus, bighead atractylodes rhizome, fructus forsythiae, ledebouriella seseloides wolff., hawthorn, radix stemonae and other compound Chinese herbs by a process of ultrasound-assisted Chinese herb extraction and two-step fermentation of multi-strain aerobic fermentation and multi-strain anaerobic fermentation. The fermented Chinese herbal immunostimulant for the flatfish has dual advantages of Chinese herbs and probiotics, and can efficiently condition intestinal health of the flatfish, treat both symptoms and root causes, strength the foundation and invigorate Qi, maintain the intestinal microecological balance and an optimal immune state, improve digestion and absorption of nutrients, improve the body immunity of the flatfish, increase the farming income and reduce farming risks; as a substituent for the antibiotics, the fermented Chinese herbal immunostimulant has no adverse effect on the flatfish and is safe to use and free of drug residue, drug resistance and other problems; therefore, the fermented Chinese herbal immunostimulant has a broad market prospect.

The invention discloses an antigen protein of avibacterium paragallinarum and application of the antigen protein. Amino acid sequences of the antigen protein are shown as SEQ ID NO.1. The invention further provides a reagent kit for detecting antibodies of the avibacterium paragallinarum and a vaccine preparation for the avibacterium paragallinarum. The reagent kit can be used for detecting the antibodies induced by outer membrane proteins of A-type thalli, B-type thalli and C-type thalli of the avibacterium paragallinarum or assisting in detecting the antibodies induced by the outer membraneproteins of the A-type thalli, the B-type thalli and the C-type thalli of the avibacterium paragallinarum. The antigen protein, the application, the reagent kit and the vaccine preparation have the advantages that immune states of chicken can be judged by the aid of detection results, so that corresponding measures can be adopted by farmers in the poultry industry; the reagent kit contains negative control serum and positive control serum, and accordingly the accuracy and the reliability of the detection results can be guaranteed; the vaccine preparation can be used for being vaccinated in thechicken, and accordingly the chicken can be effectively protected against infection of the A-type, B-type and C-type avibacterium paragallinarum.

The invention provides a microblog-based information dissemination power system, a construction method and device and a medium. The construction method comprises the following steps: dividing the states of users in the microblogs into a susceptible state, a forwarding state or/and a reading state and an immune state; constructing an information propagation dynamic model according to the change ofthe user state; and predicting the number and change trend of users in each state in the future by monitoring the accumulated forwarding quantity or/and the accumulated reading quantity of the microblogs according to the information transmission dynamic model. The microblog-based information dissemination power system, the construction method thereof, the device thereof and the medium analyze theforwarding or/and reading information published to the outside by the microblog, and predict the public opinion.

This invention provides a kind of mouse immunity state test reagent, this reagent is PBS suspension of mouse spleen cell in two inbreeding system which is stained by CFSE, the total concentration of the two kind of mouse spleen cell is 0.5-1 multiply 10 to the power 8 per ml, the proportion is 1:1, fluorescence tinction differential concentration is 1:20. Mainline the reagent in the model mouse; it can judge the immunity state of the model mouse by the change of the proportion of the two cell in this reagent when letting blood to test.

Compositions and methods for the diagnosis, prevention and treatment of immune states and disorders amenable to treatment through modulation of T cell activation are provided. In accordance with preferred embodiments, oligonucleotides are provided which are specifically hybridizable with nucleic acids encoding B7 proteins.

The invention discloses a nasal cavity Chinese medicinal washing fluid. The washing fluid is characterized by comprising tea polyphenol extract, ganoderma lucidum polysaccharides, chrysanthemum extract, biond magnolia flower extract and asarum extract, wherein the components are respectively weighed in a weight ratio of (1.8-2.2):(1.8-2.2):(1.8-2.2):1:1; and 0.9 to 2.3 percent of sodium chloride solution is added. According to the nasal cavity Chinese medicinal washing fluid, immunopathological reaction can be inhibited, bacterium and virus growth can be inhibited, nasal cavity tissue repair is promoted, and local immune state Chinese medicinal extract is improved; and sodium chloride and sodium bicarbonate are used to regulate PH value and osmotic pressure, so that the Chinese medicinal washing fluid is more in accordance with the nasal cavity regional anatomy physiological characteristics and nasal cavity mucociliary physiological characteristics. Therefore, the nasal cavity Chinese medicinal washing fluid has excellent curative effect, is suitable for various types of patients with nasal diseases, has remarkable relieving effect to acute cold stuffy nose, running nose, dizziness and other symptoms, and can shorten the disease duration and remarkably improve the living quality.