60 results about "Tissue section" patented technology

The invention relates to a CACNA1A-based mental patient heart death intelligent identification system and method, the system comprises an immunohistochemical staining device, an image acquisition unit, an image analysis unit and an intelligent identification unit, the immunohistochemical staining device is used for performing CACNA1A immunohistochemical staining on formalin-fixed paraffin-embedded heart tissue slices; the image acquisition unit is used for shooting and obtaining a heart tissue CACNA1A immunohistochemical staining image; the image analysis unit is used for processing the heart tissue CACNA1A immunohistochemical staining image so as to determine a tissue staining region and image features thereof; and the intelligent identification unit outputs an identification prediction result of heart death of the mental patient according to the image features of the tissue staining region. Compared with the prior art, the mental patient heart death identification system can intelligently and accurately identify mental patient heart death.

The invention relates to the technical field of frozen slicing, in particular to a mammal skin embedding method for a spatiomics technology, which comprises the following steps: acquiring an in-vitro skin sample which comprises an epidermal layer, a corium layer and optional subcutaneous tissue; shearing the fat in the skin sample under the condition a to remove the fat in the skin sample, and freezing and embedding the fat-removed skin sample. The mammal skin embedding method provided by the embodiment of the invention provides a standardized overall scheme for sample analysis of mammal skin and hair follicle tissues, is beneficial to obtaining tissue slices with high quality, complete morphology and good cell state, and realizes space omics sequencing with high sample utilization rate.

The invention discloses an optical positioning method and system for papillary thyroid cancer cells of a terahertz near-field system, and relates to the field of terahertz near-field imaging, and the method comprises the following steps: carrying out the imaging observation of a thyroid tissue section under a first magnification factor through a microscope, carrying out low-magnification imaging partitioning on an area where imaging observation is located according to the morphological characteristics of the cells to form partitioned images; selecting an area of suspected papillary thyroid cancer cells, performing observation and comparison under a second magnification, comparing the observed morphology with the known optical characteristics of the papillary thyroid cancer cells, and selecting an area near the area with the suspected characteristics as an optical positioning point and performing marking; and carrying out positioning according to the optical positioning point, and carrying out scanning imaging by using a terahertz near-field system. The problems that a terahertz near-field system is long in time consumption and difficult to locate when finding papillary cancer cells in multiple cells of thyroid tissue slices are mainly solved.

The invention provides a method for carrying out multi-term immunohistochemical staining by using a single tissue slice, which is characterized by comprising the following steps: S1, selecting and preparing a tissue slice to be detected, S2, analyzing pathological tissues and related information of an HE tissue slice, S3, marking, transferring, correcting and punching staining through holes of the HE tissue slice and the tissue slice to be detected, S4, preparing before immunohistochemical staining, and S5, carrying out multi-term immunohistochemical staining by using a single tissue slice. Removing the protective film on the bottom surface of the perforated transparent adhesive sticker, bonding the bottom surface of the transparent adhesive sticker with the wax mask tissues of the to-be-detected tissue slice, exposing the wax mask tissues of the to-be-detected tissue slice at the bottoms of the dyeing through holes corresponding to the transparent adhesive sticker respectively, and removing the protective film on the surface of the transparent adhesive sticker to obtain the to-be-detected tissue slice. And opening or closing the corresponding dyeing through holes by using the silica gel nail covers to form the to-be-detected tissue sample sheet with a plurality of dyeing holes. And more comprehensive detection results of a plurality of items can be obtained on only one tissue slice, so that the application range of immunohistochemical detection is expanded, the immunohistochemical detection time is shortened, the working efficiency is improved, and the detection cost is reduced.

The application discloses a kind of for carcinosarcoma evaluation section and its preparation method and application, belong to pathological technical field.The preparation method includes the following steps: reticular fiber staining: silver staining method is carried out to the reticular fiber in section tissue and is dyed;Immunohistochemical staining: AE1 / AE3 protein in section tissue is marked and dyed by immunohistochemical staining method.The section obtained by the preparation method can present two kinds of staining on a tissue section, improve the expression effect of cancer, sarcoma, carcinosarcoma, reduce the identification difficulty, and be beneficial to tumor accurate typing.

This invention relates to the field of image processing technology, specifically disclosing an AI-based 3D reconstruction method for human organ tissue slices. The method includes: acquiring a sequence of consecutive slice images and preprocessing them to generate a tissue foreground mask; using previously registered slices as references, estimating the homography matrix through a feature matching neural network and transforming it to obtain candidate registration results; calculating the pixel correlation between the candidate and the reference foreground mask, retaining those not less than a threshold and taking the largest to form a registration slice sequence; semantically segmenting the registration sequence to obtain a target structure mask sequence, and stacking it in voxels according to the interlayer spacing to output the 3D reconstruction result. By using multi-reference registration based on foreground correlation for optimal selection, the accumulation of misregistration is suppressed, and the alignment stability is improved; post-registration segmentation and voxel stacking realize multi-structure 3D reconstruction and sub-structure display. This method enhances registration reliability and supports multi-label 3D display.

The invention discloses a tissue slice in-situ hybridization pretreatment liquid and a use method, the tissue slice in-situ hybridization pretreatment liquid is characterized in that the tissue slice in-situ hybridization pretreatment liquid is composed of a compound component A solution and a surfactant component B solution, the compound component A solution and the surfactant component B solution have a proper octanol / water partition coefficient and can effectively remove endogenous fluorescent substances, and a solvent is a hydrophilic solvent; wherein the component A is one or a combination of more of bis (hexamethylene) triamine, dodecyl pyridine chloride, cyclohexylamine, isophorone diamine, N-benzylamino ethanol, N, N, N, N-tetramethyl-1, 6-hexamethylenediamine and 1, 3-bis (aminomethyl) cyclohexane; and the component B is one or a combination of more of triton X-100, tween-20, lauryl sodium sulfate and sodium dodecyl benzene sulfonate. The method is used when the FFPE slice is subjected to fluorescence in-situ hybridization or immunofluorescence experiments, autofluorescence can be reduced in a rapid removal mode, the pretreatment effect is remarkably improved, and the hybridization efficiency of samples difficult to hybridize is improved.

The application relates to a method for rapidly locating and semi-quantitatively analyzing new organic pollutants in zebrafish in vivo by mass spectrometry imaging. The method comprises the following steps: obtaining a whole zebrafish sample after exposure and water flushing; preparing a whole fish frozen section after embedding and freezing by sodium carboxymethyl cellulose (CMC); analyzing the tissue section by using desorption electrospray ionization mass spectrometry imaging (DESI-MSI); simultaneously adding standard samples with a series of concentrations on a blank glass slide, establishing a standard curve based on the average ion intensity; extracting the average ion intensity of a region of interest (ROI) in the tissue section, substituting the average ion intensity into the standard curve to calculate the concentration of the detected substance in the region, and completing semi-quantitative analysis. The application can rapidly and directly locate the distribution of new organic pollutants in different tissues of zebrafish, realize semi-quantitative evaluation of the local concentration of the tissues, and is suitable for ecological toxicology research and risk prediction of new organic pollutants.

The application discloses application of YW2301 in preparation of a medicine for resisting Burkitt lymphoma, utilizes CCK8 cell activity experiment to detect the inhibition ability of YW2301 on Raji and CA46 cell proliferation, constructs a NON / SCID subcutaneous transplantation tumor model by using a Raji cell line, evaluates the therapeutic significance of YW2301 on Burkitt lymphoma, YW2301 can effectively inhibit the tumorigenicity of Burkitt lymphoma cells and the growth speed of the transplantation tumor, and adopts immunohistochemical staining to detect the ALDH18A1 and c-Myc protein expression in a Burkitt lymphoma transplantation tumor tissue section, YW2301 can effectively down-regulate the ALDH18A1 and c-Myc protein expression in a Burkitt lymphoma tissue, and further proves that YW2301 can be used as a candidate small molecule for targeted treatment of Burkitt lymphoma.

The utility model relates to biological tissue sample microscopic imaging technical field provides a slice formula three -dimensional tomography optical microscopic system, it includes ultrathin section module, optical imaging module and data acquisition module, ultrathin section module is used for realizing to sample's scanning formula section, ultrathin section module includes processing groove, cutter assembly, processing liquid circulating device and flush device, cutter assembly has the upper surface of slanting upwards, and the tissue slice obtained after sample is sliced via cutter assembly is exported from the upper surface;The liquid inlet of processing liquid circulating device is set up in the section export direction corresponding the upper surface, and processing liquid circulating device is used for forming the water flow suction field on the upper surface;The water outlet of flush device corresponds the upper end surface of sample and is set up towards cutter assembly's direction, and flush device is used for forming the water flow impact force along section export direction on the upper end surface of sample and the upper surface, the accuracy and reliability of the system to biological sample three -dimensional structure information acquisition are improved.

The application provides a mass spectrometry imaging detection method for cholesterol in a tissue slice and application thereof, and belongs to the technical field of mass spectrometry detection. The method at least comprises the following steps: synthesizing a derivatization reagent, preparing a derivatization system, spraying a cholesterol extraction solvent and a derivatization system solution to the surface of a prepared tissue slice to obtain a derivatization product of cholesterol; and performing matrix spraying on the tissue slice after derivatization and performing MALDI mass spectrometry imaging analysis. The application can effectively improve the detection sensitivity of cholesterol in a tissue, realizes visual analysis of cholesterol in a biological tissue, and therefore has good practical popularization and application value.

This invention belongs to the field of renal pathology testing technology and discloses a method for detecting type IV collagen expression in renal biopsy tissue, comprising the following steps: dewaxing and hydrating paraffin sections of renal biopsy tissue to obtain processed tissue sections; adding the processed tissue sections to a repair solution, heating to boiling, and then cooling to room temperature to obtain repaired tissue sections; adding protease to the repaired tissue sections for enzymatic digestion to obtain enzymatically digested tissue sections; adding a primary antibody to the enzymatically digested tissue sections for preliminary incubation, followed by adding a fluorescein-labeled secondary antibody against the primary antibody for further incubation. This method for detecting type IV collagen expression in renal biopsy tissue is applicable to cases where renal biopsy tissue lacks glomeruli or has too few glomeruli to detect type IV collagen expression. It has high specificity and can provide a new detection method for Alport syndrome in cases where renal biopsy tissue lacks glomeruli.

The utility model relates to kit technical field, and disclose a kind of tissue section hyaluronic acid staining kit, including kit, the top of the kit is provided with box cover, the inside of the kit is provided with sponge pad, the inner wall of the kit is provided with fixing device;The fixing device includes hydraulic cylinder, the bottom of the hydraulic cylinder is fixedly connected in the inside of sponge pad, the inside of one end of the hydraulic cylinder is slidably connected with force bar by piston, the inside of the other end of the hydraulic cylinder is slidably connected with hydraulic ram by another piston.The utility model through fixing device, so that rubber bar will be because the extrusion compression spring of reagent bottle moves to through slot when rubber bar contacts reagent bottle, and then one end of rubber bar can be fixed to reagent bottle when it is against through slot, different number of reagent bottles can be fixed by telescopic rod, to prevent from shaking when carrying and make reagent bottle collide, cause breakage.

This invention relates to the field of ultrasound imaging, and in particular to a wearable ultrasound transducer with adjustable detection angle. The transducer includes a transducer housing, a connecting ball within the housing, and multiple elastic clamping members arranged horizontally around the connecting ball. The connecting ball has an upper connecting rod at its upper part and a lower connecting rod at its lower part, with an ultrasound transducer at the end of the lower connecting rod. An upper and lower isolation membrane are located in the lower part of the transducer housing, with the upper isolation membrane positioned between the connecting ball and the ultrasound transducer. Gaps exist between the ultrasound transducer and both the upper and lower isolation membranes, and these gaps are filled with a medium whose acoustic impedance coefficient is close to that of human tissue. When worn, the ultrasound transducer structure of this invention allows for manual adjustment of the imaging angle with internal human tissue, enabling continuous monitoring of a specific tissue section and avoiding the monitoring interruption problem of traditional wearable ultrasound transducers due to human movement and changes in body position.

This invention discloses a preprocessing method for the spatial transcriptome of maize anthers based on morphological restoration and precise permeation. The method includes: attaching and fixing maize anther tissue sections onto a spatial transcriptome capture chip; staining with a preferred concentration (1 / 200) of fluorescent whitening agent (FB28), followed by moist incubation to allow the anther exowall cells to absorb water and reshape; imaging and localization while maintaining chip surface hydration throughout the process; precise permeation treatment for 7 minutes using a permeation reagent; and finally, in situ cDNA synthesis. This invention effectively overcomes the localization challenge caused by maize anther tissue shrinkage through the synergistic effect of staining and restoration processes. Combined with optimized permeation parameters, it solves the permeation problem at different developmental stages of anthers, significantly improving the spatial resolution of sequencing data and making it suitable for spatial transcriptome studies of the entire developmental cycle of maize anthers.

This invention provides a multimodal microscopic imaging integrated detection system, relating to the field of microscopic optical detection technology. It includes a bright-field microscopic imaging module sharing a tissue section sampling optical path assembly, and at least two of three modules: a polarized light microscopic imaging module, a fluorescence microscopic imaging module, and a micro-Raman spectroscopy detection module. The bright-field, polarized light, and fluorescence microscopic imaging modules share an imaging optical path assembly. The micro-Raman spectroscopy detection module includes a Raman spectrometer and a laser for irradiating tissue sections to excite Raman scattering light. The beneficial effect of this invention is that, based on bright-field microscopic imaging, it introduces multiple characteristic imaging or spectroscopic technologies such as polarized light imaging, fluorescence imaging, and Raman spectroscopy. Through multimodal information complementarity, it enriches the differential information between cancerous tissues and normal, inflammatory lesions, etc., significantly improving the speed and efficiency of pathological examination and enabling rapid and accurate intraoperative cancer diagnosis.

The invention provides repair liquid suitable for a full-automatic immunohistochemical staining instrument and a use method of the repair liquid, and particularly relates to the technical field of immunodetection. The repairing liquid is prepared from the following substances in percentage by mass: 0.1 to 1 percent of Tris, 0.01 to 0.1 percent of EDTA (Ethylene Diamine Tetraacetic Acid), 0.5 to 1 percent of glucan-20, 15 to 25 percent of ethylene glycol, 0.01 to 0.1 percent of Tween-20 and the balance of pure water; the temperature is set to be 60-100 DEG C in a computer program, the adding amount of the repairing liquid is set to be 150-250 microliters, and the reaction time is set to be 30-50 min; a tissue slice sample is used, and the instrument automatically runs; the preparation method is not easy to volatilize, environment-friendly, simple in storage condition, easy to clean, ideal in preparation strength and background when matched with instruments, and capable of meeting the high-throughput and high-quality preparation requirements of hospitals.

The present application relates to the technical field of image recognition, and particularly relates to a virtual staining method for immunohistochemical section images, comprising: obtaining a pair of training images, extracting a set of two-dimensional coordinates of cell nucleus centers and a normalized gray integral vector, and taking the set of two-dimensional coordinates of cell nucleus centers and the normalized gray integral vector as edge distribution constraints to obtain a set of two-dimensional displacement vectors of cells; determining a local adjacency relationship based on the center coordinates, combining the set of displacement vectors to extract a set of adjacent coordinate offset difference scalars, and reconstructing the set of adjacent coordinate offset difference scalars into a two-dimensional spatial misplacement cost matrix; converting the cost matrix into a pixel-level weight mask, locally truncating a model prediction error to train a generation model; and finally converting an H&E image into a virtual IHC image by using the model. The present application effectively blocks an error gradient return caused by physical misplacement, eliminates false positive coloring, and significantly improves clinical reliability.

The invention discloses a method for synchronously marking germ cells and interstitial cells in paralichthys olivaceus testis tissues, which comprises the following steps: cloning paralichthys olivaceus MIR17HG-a (long non-coding RNA) by using a probe primer, and extracting plasmids to obtain target plasmids; the method comprises the following steps of: synthesizing a paralichthys olivaceus MIR17HG-a probe through SP6 / T7 RNA polymerase, preparing paralichthys olivaceus testis tissues into tissue slices, and performing fluorescence in-situ hybridization on the tissue slices by using the paralichthys olivaceus MIR17HG-a probe, so that the paralichthys olivaceus MIR17HG-a probe can synchronously mark germ cells and interstitial cells in the paralichthys olivaceus testis tissues. The defect that a traditional marking technology needs combined marking or multi-time step-by-step marking is overcome, and the blank of synchronous marking of germ cells and interstitial cells in paralichthys olivaceus testis tissue at present is filled.

Techniques for obtaining a synthetic histochemically stained image from a multiplexed immunofluorescence (MPX) image may include producing an N-channel input image that is based on information from each of M channels of an MPX image of a tissue section, where M and N are positive integers and N is less than or equal to M; and generating a synthetic image by processing the N-channel input image using a generator network, the generator network having been trained using a training data set that includes a plurality of pairs of images. The synthetic image depicts a tissue section stained with at least one histochemical stain. Each pair of images of the plurality of pairs of images includes an N-channel image, produced from an MPX image of a first section of a tissue, and an image of a second section of the tissue stained with the at least one histochemical stain.

Embodiments of the present disclosure generally relate to the field of biological sample processing, and in particular to an automatic pathological tissue sectioning machine, comprising a section stripping device, the section stripping device is provided with a first temperature control device, the control temperature of the first temperature control device is not higher than 20 DEG C; a section transmission device, comprising an adhesive tape for carrying a target section in an unwound state, the adhesive tape is composed of a material that can be dissolved in water or alcohol, and the adhesive tape comprises a support layer and an adhesive layer with a temperature-sensitive adhesive, the adhesive force of the temperature-sensitive adhesive at a temperature lower than 20 DEG C is higher than the adhesive force of the temperature-sensitive adhesive at a temperature of 35 DEG C to 45 DEG C; and a section separation device, provided with a second temperature control device, the control temperature of the second temperature control device is 35 DEG C to 45 DEG C. The wax block embedded with pathological tissue can be automatically sectioned to produce a white section, replacing the traditional water bath sectioning, fishing sectioning and baking sectioning steps, simplifying the white section production process and reducing manual operation.

The present invention relates to an ion source (00) for high-resolution mass spectrometry imaging and a mass spectrometry imaging system. The ion source (00) comprises a first stage (001), a second stage (002), an ionization electrode (003), a high-voltage power supply (004), a scanning probe (005), a microfluidic pump (006), and a control device (007); a first holder (011) is provided on the first stage (001); the first holder (011) is used for holding a glass slide (110); a tissue section sample (220) on the front surface of the glass slide (110) held by the first holder (011) faces a sample inlet (330) of a mass spectrometer (33); a second holder (012) is provided on the second stage (002); the second holder (012) is used for holding the scanning probe (005); the scanning probe (005) is connected to the microfluidic pump (006) by means of a conduit (008); the ionization electrode (003) directly faces the sample inlet (330) of the mass spectrometer (33), and the ionization electrode (003) is located on the back surface of the glass slide (110) held by the first holder (011); the ionization electrode (003) is electrically connected to the high-voltage power supply (004); and the control device (007) is separately electrically connected to the first stage (001), the second stage (002), the high-voltage power supply (004), and the microfluidic pump (006). In this way, a picoliter droplet desorption electrospray ion source (00) having higher sensitivity and spatial resolution in a mass spectrometry imaging technique is realized.

The invention discloses a gynecological tumor sample tissue slicing device which is characterized in that a sliced tissue main body comprises a working table, a blade moving seat, a slicing knife, a material receiving cover, a control table and a loading moving seat, the blade moving seat is slidably mounted in the middle of the upper surface of the working table, and the material receiving cover is slidably mounted on the inner side of the blade moving seat in an embedded manner; the control table is fixedly installed on the upper surface of the workbench. A supporting frame is fixedly installed in the middle of the upper surface of the workbench. The sealed purification mechanism is fixedly mounted on the upper surface of the workbench; and the slice cleaning mechanism is connected to the side positions of the object carrying moving seat and the blade moving seat. According to the gynecological tumor sample tissue slicing device, the novel structural design is adopted, gas in the slicing environment can be purified and sterilized through the device, a slicing knife cleaning treatment structure is arranged in the device, continuous habitual slicing processing of the device is achieved, and the slicing processing efficiency is improved.

The invention belongs to the technical field of biomedicine, and particularly relates to CCL25 and application of the CCL25 combined with CD8 + T cells and M1 macrophages to evaluation of a liver cancer immune microenvironment and prediction of a liver cancer immunotherapy curative effect. Based on TCGA liver cancer tissue transcriptome data, liver cancer tissue slice multiple immunohistochemistry, liver cancer tissue MRS score calculation and ROC curve multiple verification, it is proved that the chemotactic factor CCL25 expression level can serve as a biomarker for evaluating the liver cancer immune microenvironment. Furthermore, by analyzing data of liver cancer patients receiving anti-PD-1 treatment, a combined prediction model containing the CCL25 expression level, the CD8 + T cell infiltration level and the M1 macrophage infiltration level is established, and the prediction effect of the combined prediction model is better than that of independent use of immune checkpoint indexes. The technical scheme of the invention provides a potential new method for predicting the curative effect of immunotherapy of clinical liver cancer patients, and has a certain clinical application prospect.

The invention discloses a virtual immunohistochemical staining method, device and equipment for a tissue slice and a medium, and relates to the field of computational pathology counting. E, constructing a cell map from the dyed image to generate corrected IHC semantic features of each node; generating a history Hamp by using an image generator; e, dyeing a historical virtual IHC image corresponding to the image; using the historical real IHC image and the historical virtual IHC image to optimize the parameters of the double-branch discriminator; optimizing image generator parameters by using a discrimination result of the historical virtual IHC image output by the discriminator, the corrected IHC semantic features and semantic features in the historical virtual IHC image to obtain a new image generator network, and performing optimization for multiple times to obtain a target image generator; outputting the Hamp of the tissue section to be dyed by using the generator; and E, dyeing the target virtual IHC image of the image. And performing virtual IHC with clear texture and accurate pathological features on the tissue slices.

The present application relates to a kind of tissue slice electrostatic collection device and tissue slice collection system, including section transmission component, the section transmission component includes the polymer film transmission belt for electrostatically adsorbing tissue slice and transporting tissue slice;Charging device, the charging device is close to the polymer film transmission belt arrangement, for making the polymer film transmission belt on static electricity.Based on the tissue slice electrostatic collection device and tissue slice collection system of the present application, using the nature of electrostatically adsorbing small object, electrostatic generation technology is combined with the polymer film transmission belt that can be stably charged and applied to the field of biological tissue slice collection, the collection of tissue slice is realized, and the secondary transfer of biological tissue slice is facilitated, and the damage to sample is small, and pollution is less.

The application discloses a hyperspectral microscopic imaging analysis system for label-free tumor tissue, comprising a super surface slide, a hyperspectral imaging system and a medical optical analysis algorithm system connected in sequence; the super surface slide comprises a glass substrate, a super surface microstructure and a biocompatible coating arranged in sequence from bottom to top, and a tissue slice is placed on the super surface microstructure. The application aims at the problems of high cost, low sensitivity, difficulty in early diagnosis and excessive dependence on doctors' experience of the current traditional cancer diagnosis technology, realizes the differentiation and identification of tumor tissue in a label-free manner in the visible light range, develops a spectral imaging system with fast, dynamic and high spectral resolution together with an image sensor, extracts spectral information in the form of image position to assist doctors in pathological diagnosis, and reduces the possibility of missed diagnosis or misdiagnosis.

The utility model discloses antigen repair equipment. The antigen repair equipment comprises a heating cavity and an incubation cavity, two symmetrically arranged and movable semiconductor chilling plate modules are arranged in the heating cavity, and the semiconductor chilling plate modules can move relatively; when the antigen repair equipment is in a heating working condition, the two semiconductor chilling plate modules move in opposite directions, so that the second sides are tightly attached to the two sides of a container filled with repair liquid outside the tissue slice, and the container is directly heated; the air collecting shell is used for collecting cold air of the semiconductor chilling plate module and conveying the cold air to the incubation cavity through the first hose, and the incubation cavity is used for pre-cooling or refrigerating tissue slices in the incubation cavity. According to the utility model, the antigen repair efficiency can be greatly improved, the film making probability can be reduced, and the antigen repair quality is improved.

The invention discloses a multiple fluorescent staining method for multiple protein targets, and relates to the technical field of multiple immunofluorescent staining. According to the method provided by the invention, the number of dyeable protein targets in the tissue slice can be greatly increased, multiple fluorescent staining steps can be simplified, and by adopting the method provided by the invention, imaging can be carried out after all the targets are directly stained on the basis of one tissue slice. The number of tissue slices required by dyeing imaging is reduced, dyeing of more than 6 protein targets can be completed by only one tissue slice, and errors caused by slice continuity differences are avoided. In addition, repeated elution and re-dyeing are not needed, so that the problems of antigen loss and weakening and the like caused by repeated elution are avoided.

The application provides a mass spectrometry imaging detection method for a compound containing an ortho-diphenol hydroxyl functional group in a tissue and application thereof, and belongs to the technical field of mass spectrometry detection. The method at least comprises: synthesizing a derivatization reagent in a solution, spraying the derivatization reagent solution to the surface of a prepared tissue section to obtain a derivatization product of the compound containing the ortho-diphenol hydroxyl functional group; and performing matrix spraying on the tissue section after derivatization, and then performing MALDI mass spectrometry imaging analysis. The application can effectively improve the detection sensitivity of the compound containing the ortho-diphenol hydroxyl functional group in the tissue, realizes the visual analysis of the compound containing the ortho-diphenol hydroxyl functional group in a biological tissue, such as an endogenous metabolite and an exogenous drug, and therefore has good practical popularization and application value.