142 results about "Tissue section" patented technology

The invention relates to a CACNA1A-based mental patient heart death intelligent identification system and method, the system comprises an immunohistochemical staining device, an image acquisition unit, an image analysis unit and an intelligent identification unit, the immunohistochemical staining device is used for performing CACNA1A immunohistochemical staining on formalin-fixed paraffin-embedded heart tissue slices; the image acquisition unit is used for shooting and obtaining a heart tissue CACNA1A immunohistochemical staining image; the image analysis unit is used for processing the heart tissue CACNA1A immunohistochemical staining image so as to determine a tissue staining region and image features thereof; and the intelligent identification unit outputs an identification prediction result of heart death of the mental patient according to the image features of the tissue staining region. Compared with the prior art, the mental patient heart death identification system can intelligently and accurately identify mental patient heart death.

The invention discloses a method for researching the specific distribution of a multi-component complex system in animal tissues based on mass spectrum imaging and multivariate statistical analysis, which comprises the following steps: treating the multi-component complex system, performing animal administration for a certain time, killing the animal, taking the animal tissues, slicing the animal tissues, and taking out the sliced animal tissues; and performing mass spectrum imaging analysis and multivariate statistical analysis to obtain a specific distribution result of the multi-component complex system in the animal tissue. On the basis of a frozen section technology and a mass spectrum imaging technology, a DESI-MSI analysis method of the whole mouse tissue section is established, and in-situ distribution characteristics of the active ingredients of the Anefortia divaricata on the brain tissue section are disclosed by combining multivariate statistical analysis.

The invention discloses a bioelectrical impedance tumor identification method based on multi-modal fusion. The method comprises the following steps: S1, acquiring electrical impedance signal data and tissue slice image data of a tumor sample; the tissue slice image data are pathological slice images corresponding to tumor tissues; s2, inputting the electrical impedance signal data and the tissue slice image data into a multi-modal feature coding module for asymmetric feature coding; s3, performing splicing and weighted fusion on the global semantic feature vector and the image semantic feature vector to form a multi-modal feature vector; and S4, inputting the multi-modal feature vector into a decoding network for classification prediction, and outputting a tumor identification result. According to the method, an asymmetric coding structure and a weighted fusion mechanism are constructed by fusing the electric signal modality and the image modality, so that the utilization efficiency and the feature discrimination capability of multi-modal information can be effectively improved, and the accuracy and the reliability of tumor recognition are remarkably improved.

The invention relates to the technical field of frozen slicing, in particular to a mammal skin embedding method for a spatiomics technology, which comprises the following steps: acquiring an in-vitro skin sample which comprises an epidermal layer, a corium layer and optional subcutaneous tissue; shearing the fat in the skin sample under the condition a to remove the fat in the skin sample, and freezing and embedding the fat-removed skin sample. The mammal skin embedding method provided by the embodiment of the invention provides a standardized overall scheme for sample analysis of mammal skin and hair follicle tissues, is beneficial to obtaining tissue slices with high quality, complete morphology and good cell state, and realizes space omics sequencing with high sample utilization rate.

The invention discloses an immunostaining reagent for MHC-II expression detection of a gastric cancer biopsy sample, and belongs to the field of bioengineering. The invention establishes a preparation method of an immunohistochemical staining section. The preparation method specifically comprises the following steps: step 1, early-stage treatment: baking a tissue section, dewaxing, rehydrating, subsequently adding an antigen solution for repairing, adding an endogenous enzyme inhibitor for incubation, adding a sealing agent for sealing and the like; step 2, antibody incubation: adding a first antibody and a second antibody for incubation; and step 3, post-treatment: dyeing, re-dyeing, bluing, sealing and the like. A mixed buffer solution is used as an endogenous enzyme inhibitor, so that the background staining intensity is effectively reduced, and the staining sensitivity and specificity are improved; meanwhile, a staining result interpretation standard is established, it is proved that MHC-II expression is remarkably related to pathological remission of the gastric cancer patient, and an evaluation method is provided for pathological remission of the gastric cancer patient.

The application discloses application of a small-molecule drug indisulam in preparation of a drug for treating autoimmune diseases, and further verifies safety of the molecule, long-term continuous use of the drug does not cause weight loss of animals, and weight change has no difference with normal control mice; tissue sections of multiple organs show that the drug has no obvious toxic side effects on each organ. In addition, the drug can promote differentiation of regulatory T cells, but does not affect functions of other immune cells. Therefore, the drug is used for treating autoimmune diseases, and good effect is found.

The utility model discloses a small animal pathological tissue section bleaching and drying device which comprises an ultrasonic cleaning box, supporting legs are fixedly installed at the four corners of the outer surface of the lower end of the ultrasonic cleaning box, supporting columns are fixedly installed on the left side and the right side of the upper portion of the outer surface of the rear end of the ultrasonic cleaning box, and the number of the supporting columns is two sets. A drying assembly is fixedly mounted on the upper portion between the two supporting columns and comprises a box plate, an air outlet, a fan, a fixing frame, an air inlet pipe, a hollow protective shell, an air outlet pipe, a heater, an air filter element and a protective net. According to the small animal pathological tissue section bleaching and drying device, through the arrangement of the section lifting assembly, a mesh basket containing sections can be conveniently driven to be placed in the ultrasonic cleaning box, use is convenient, the treatment efficiency is improved, the mesh basket is driven to be close to the lower portion of the box plate through the section lifting assembly, and the section lifting assembly is used for lifting the sections. And the slices can be heated and dried through the drying assembly, and use is convenient.

The invention discloses an optical positioning method and system for papillary thyroid cancer cells of a terahertz near-field system, and relates to the field of terahertz near-field imaging, and the method comprises the following steps: carrying out the imaging observation of a thyroid tissue section under a first magnification factor through a microscope, carrying out low-magnification imaging partitioning on an area where imaging observation is located according to the morphological characteristics of the cells to form partitioned images; selecting an area of suspected papillary thyroid cancer cells, performing observation and comparison under a second magnification, comparing the observed morphology with the known optical characteristics of the papillary thyroid cancer cells, and selecting an area near the area with the suspected characteristics as an optical positioning point and performing marking; and carrying out positioning according to the optical positioning point, and carrying out scanning imaging by using a terahertz near-field system. The problems that a terahertz near-field system is long in time consumption and difficult to locate when finding papillary cancer cells in multiple cells of thyroid tissue slices are mainly solved.

The invention discloses an in-situ derivatization mass spectrometry imaging method, which belongs to the technical field of mass spectrometry imaging detection, and comprises the following steps: preparing a sample slice and attaching the sample slice to a conductive glass slide; the method comprises the following steps: dissolving a derivatization reagent and a catalyst in a volatile organic solvent to prepare a derivatization reagent system solution, and spraying the derivatization reagent system solution on the surface of the tissue slice for derivatization reaction; coating a matrix on the surface of the derivatization slice, and identifying and analyzing by using an MALDI-MSI method; the structure of the derivatization reagent is shown in the specification. The derivatization reagent can perform mild and rapid in-situ derivatization on trace carbonyl-containing compounds in biological tissues, significantly improve the mass spectrum signal response of target compound molecules and significantly inhibit the delocalization (diffusion) phenomenon of analytes, and the MALDI-MSI technology is adopted to analyze the derivatized tissue slices. High-spatial-resolution in-situ visualization characterization of trace carbonyl-containing compounds in biological tissue slices is realized, and the problems in the prior art are effectively solved.

The invention discloses an illumination cage-removing probe as well as a preparation method and application thereof. The illumination cage-removing probe has a structure as shown in a formula I; the illumination cage removal probe has a very high illumination cage removal rate in a non-polar solvent, and has a very low illumination cage removal rate in a water phase. The solvent polarity-sensitive illumination decaging probe can be used for proximity labeling of lipid droplet surrounding proteins in fatty liver pathological tissue slices. # imgabs0 #

The invention provides a method for carrying out multi-term immunohistochemical staining by using a single tissue slice, which is characterized by comprising the following steps: S1, selecting and preparing a tissue slice to be detected, S2, analyzing pathological tissues and related information of an HE tissue slice, S3, marking, transferring, correcting and punching staining through holes of the HE tissue slice and the tissue slice to be detected, S4, preparing before immunohistochemical staining, and S5, carrying out multi-term immunohistochemical staining by using a single tissue slice. Removing the protective film on the bottom surface of the perforated transparent adhesive sticker, bonding the bottom surface of the transparent adhesive sticker with the wax mask tissues of the to-be-detected tissue slice, exposing the wax mask tissues of the to-be-detected tissue slice at the bottoms of the dyeing through holes corresponding to the transparent adhesive sticker respectively, and removing the protective film on the surface of the transparent adhesive sticker to obtain the to-be-detected tissue slice. And opening or closing the corresponding dyeing through holes by using the silica gel nail covers to form the to-be-detected tissue sample sheet with a plurality of dyeing holes. And more comprehensive detection results of a plurality of items can be obtained on only one tissue slice, so that the application range of immunohistochemical detection is expanded, the immunohistochemical detection time is shortened, the working efficiency is improved, and the detection cost is reduced.

A device for preparing a tissue for analysis includes a first conduit adapted to transport at least one solvent, the first conduit having a distal opening that delivers the at least one solvent to a selected location on the tissue, a second conduit adapted to collect a sample comprising the at least one solvent and an analyte / metabolite from the tissue and transport the sample to at least one of a waste disposal, a storage container, and a sample analyzer. The device may be configured to directly sample an embedded tissue, and may include a heater that heats the at least one solvent to a temperature that melts the embedding medium, wherein the collected sample comprises the at least one solvent, liquified embedding medium, and an analyte / metabolite. The device allows direct analysis of the tissue at the selected location, without removing the embedding medium from the entire tissue section.

The application discloses a kind of for carcinosarcoma evaluation section and its preparation method and application, belong to pathological technical field.The preparation method includes the following steps: reticular fiber staining: silver staining method is carried out to the reticular fiber in section tissue and is dyed;Immunohistochemical staining: AE1 / AE3 protein in section tissue is marked and dyed by immunohistochemical staining method.The section obtained by the preparation method can present two kinds of staining on a tissue section, improve the expression effect of cancer, sarcoma, carcinosarcoma, reduce the identification difficulty, and be beneficial to tumor accurate typing.

A method for detecting a target of interest in a tissue sample is disclosed. The method includes: a) obtaining or having obtained a tissue sample, the tissue sample comprising DNA, non-RNA molecules, and RNA, the RNA comprising the target of interest; b) permeabilizing the tissue sample; c) substantially degrading DNA in the tissue sample; d) introducing primers or probes specific for the target of interest into the tissue sample, wherein the primers or probes bind to the target of interest; and e) detecting the target of interest in the tissue sample by detecting the primers or probes bound to the target of interest. A method for producing a hydrogel within the tissue sample is also disclosed.

The present application relates to a kind of ganoderma lucidum spore as carrier paclitaxel ganoderma lucidum spore oil self-nanoemulsion composite microparticle and its preparation method, belong to medical technology field.The pretreated ganoderma lucidum spore (GLS) is obtained by process screening, and it is used as drug carrier, and the paclitaxel ganoderma lucidum spore oil self-nanoemulsion (PGS) is loaded therein, and paclitaxel composite microparticle (PGS@GLS) is obtained.Release curve in vitro shows that PGS@GLS has the characteristics of slow release after reaching intestinal tract;In vivo pharmacodynamics experiment shows that PGS@GLS can significantly inhibit the development and metastasis of colorectal cancer in mice;Tumor tissue section immunofluorescence analysis shows that PGS@GLS can effectively exert the therapeutic effect on colon cancer.Paclitaxel self-nanoemulsion ganoderma lucidum spore composite microparticle promotes cancer immune cycle by inducing immunogenic cell death and immune regulation, significantly improves the treatment effect of colon cancer, and has good biological safety.

This invention relates to the field of image processing technology, specifically disclosing an AI-based 3D reconstruction method for human organ tissue slices. The method includes: acquiring a sequence of consecutive slice images and preprocessing them to generate a tissue foreground mask; using previously registered slices as references, estimating the homography matrix through a feature matching neural network and transforming it to obtain candidate registration results; calculating the pixel correlation between the candidate and the reference foreground mask, retaining those not less than a threshold and taking the largest to form a registration slice sequence; semantically segmenting the registration sequence to obtain a target structure mask sequence, and stacking it in voxels according to the interlayer spacing to output the 3D reconstruction result. By using multi-reference registration based on foreground correlation for optimal selection, the accumulation of misregistration is suppressed, and the alignment stability is improved; post-registration segmentation and voxel stacking realize multi-structure 3D reconstruction and sub-structure display. This method enhances registration reliability and supports multi-label 3D display.

The invention discloses a method and device for identifying helicobacter pylori based on artificial intelligence fluorescence imaging, and relates to the technical field of artificial intelligence medical detection. Comprising the following steps: S1, carrying out slide preparation treatment on a cytological sample or a tissue slice sample, and processing the slide preparation through an immunofluorescent staining reagent to obtain a helicobacter pylori fluorescent staining picture; s2, outputting and obtaining a helicobacter pylori picture with a rectangular frame identifier through a helicobacter pylori identifier neural network model; and S3, according to the coordinate information of the rectangular frame, obtaining the coordinate information of the helicobacter pylori in the fluorescent staining picture, and splicing the helicobacter pylori pictures to obtain a spliced picture. According to the method, the helicobacter pylori is marked automatically through the helicobacter pylori identification neural network model, the problems of subjectivity and time consumption of traditional manual microscopic examination are avoided, meanwhile, the positions of thalli are accurately marked, positive probability data are output, and then the detection efficiency is improved.

The invention discloses a tissue slice in-situ hybridization pretreatment liquid and a use method, the tissue slice in-situ hybridization pretreatment liquid is characterized in that the tissue slice in-situ hybridization pretreatment liquid is composed of a compound component A solution and a surfactant component B solution, the compound component A solution and the surfactant component B solution have a proper octanol / water partition coefficient and can effectively remove endogenous fluorescent substances, and a solvent is a hydrophilic solvent; wherein the component A is one or a combination of more of bis (hexamethylene) triamine, dodecyl pyridine chloride, cyclohexylamine, isophorone diamine, N-benzylamino ethanol, N, N, N, N-tetramethyl-1, 6-hexamethylenediamine and 1, 3-bis (aminomethyl) cyclohexane; and the component B is one or a combination of more of triton X-100, tween-20, lauryl sodium sulfate and sodium dodecyl benzene sulfonate. The method is used when the FFPE slice is subjected to fluorescence in-situ hybridization or immunofluorescence experiments, autofluorescence can be reduced in a rapid removal mode, the pretreatment effect is remarkably improved, and the hybridization efficiency of samples difficult to hybridize is improved.

The present application describes a method for reducing background fluorescence in single molecule fluorescence in situ hybridization applications, wherein first Sudan Black B is applied to formalin-fixed, paraffin-embedded tissue sections and thereafter the single molecule fluorescence in situ hybridization application is carried out. Furthermore, there is described the use of Sudan Black B for reducing background fluorescence in single molecule fluorescence in situ hybridization applications.

The application relates to a method for rapidly locating and semi-quantitatively analyzing new organic pollutants in zebrafish in vivo by mass spectrometry imaging. The method comprises the following steps: obtaining a whole zebrafish sample after exposure and water flushing; preparing a whole fish frozen section after embedding and freezing by sodium carboxymethyl cellulose (CMC); analyzing the tissue section by using desorption electrospray ionization mass spectrometry imaging (DESI-MSI); simultaneously adding standard samples with a series of concentrations on a blank glass slide, establishing a standard curve based on the average ion intensity; extracting the average ion intensity of a region of interest (ROI) in the tissue section, substituting the average ion intensity into the standard curve to calculate the concentration of the detected substance in the region, and completing semi-quantitative analysis. The application can rapidly and directly locate the distribution of new organic pollutants in different tissues of zebrafish, realize semi-quantitative evaluation of the local concentration of the tissues, and is suitable for ecological toxicology research and risk prediction of new organic pollutants.

The invention relates to a kit for simultaneously detecting angiogenesis and plasma cell infiltration in lymphatic tissue and a preparation method and application of the kit, the kit comprises quantum well-antibody conjugates CQWs-525-VEGF, CQWs-525-ACTA2 and CQWs-585-CD138 and CQWs-625-Mum1, and the kit can be used for staining a lymphatic tissue section; by marking expression conditions of VEGF, ACTA2, CD138 and Mum1, the angiogenesis and plasma cell infiltration degree in the lymphatic tissue is evaluated. The quantum sheet has the characteristics of wide absorption spectrum, narrow light-emitting spectrum, high brightness and difficulty in quenching, and is used for marking blood vessels and plasma cells in tissues after being coupled with an antibody, so that quantitative analysis can be realized and spatial position distribution can be observed at the same time, the immune microenvironment of the tissues can be more accurately and effectively detected, and the immune state and immune function of the organism can be further evaluated.

The invention relates to the technical field of cell tissue sampling, and discloses a cancer tissue slice sampling device for tumor immunoassay, the cancer tissue slice sampling device comprises a case, a wax coating constraint assembly, a slice shifting assembly, a slice linear driver, a slice cutter, a feeding linear motor and a lifting bracket, and the wax coating constraint assembly is located in the case. The invention particularly provides a cancer tissue slice sampling device for tumor immunoassay, which can stably position a wax coated sample and can take slices one by one after integral cutting.

The application discloses application of YW2301 in preparation of a medicine for resisting Burkitt lymphoma, utilizes CCK8 cell activity experiment to detect the inhibition ability of YW2301 on Raji and CA46 cell proliferation, constructs a NON / SCID subcutaneous transplantation tumor model by using a Raji cell line, evaluates the therapeutic significance of YW2301 on Burkitt lymphoma, YW2301 can effectively inhibit the tumorigenicity of Burkitt lymphoma cells and the growth speed of the transplantation tumor, and adopts immunohistochemical staining to detect the ALDH18A1 and c-Myc protein expression in a Burkitt lymphoma transplantation tumor tissue section, YW2301 can effectively down-regulate the ALDH18A1 and c-Myc protein expression in a Burkitt lymphoma tissue, and further proves that YW2301 can be used as a candidate small molecule for targeted treatment of Burkitt lymphoma.

The invention relates to methods of in situ detection of a nucleic acid variation of a target nucleic acid in a sample, including single nucleotide variations, multi-nucleotide variations or splice sites. The method can comprise the steps of contacting the sample with a probe that detects the nucleic acid variation or splice site and a neighbor probe; contacting the sample with pre-amplifiers that bind to the nucleic acid variation probe or splice site probe and neighbor probe, respectively; contacting the sample with a collaboration amplifier that binds to the pre-amplifiers; and contacting the sample with a label probe system, wherein hybridization of the components forms a signal generating complex (SGC) comprising a target nucleic acid with the nucleic acid variation or splice site, the probes and amplifiers; and detecting in situ signal from the SGC on the sample. The invention also provides samples, tissue slides, and kits relating to detection of nucleic acid variations, including single nucleotide variations, multi-nucleotide variations or splice sites, of a target nucleic acid.

The invention relates to a 177Lu-DOTATATE peptide receptor radionuclide curative effect prediction model and application thereof, the model is used for performing multicolor immunofluorescent staining on tissue slices of a neuroendocrine tumor (NET) patient, and the model at least comprises specific staining antibodies of cytokeratin (CK +) cells, CD8 cells and TIM-3 molecules; then carrying out image acquisition and analysis, and identifying two positive cells, namely CK < + > and CD8 < + > TIM-3 < + >; calculating an under-curve area difference AUC (CK) (CD8 + TIM-3 +) between an observation curve of a nearest neighbor distance accumulation function (G function) between CK + and CD8 + TIM-3 + and a Poisson distribution curve according to an R language software package, determining a response scoring formula and threshold division based on single-factor logistic regression of PRRT response degree of case data, and substituting the difference into the response scoring formula, and the response score can be used for curative effect prediction.

The utility model discloses a container box for immunohistochemical antigen repair, which relates to the technical field of biomedicine and comprises a box body and a sealing cover, the box body is used for accommodating tissue slices and antigen repair liquid, and the sealing cover is connected to a top opening of the box body. A supporting frame used for supporting tissue slices is arranged in a cavity of the box body. The box body, the sealing cover and the supporting frame are all made of materials suitable for ultrasonic conduction. The device is reasonable in layout, compact in structure and convenient to use, the ultrasonic treatment requirement is met through material selection, the antigen repair efficiency and the dyeing quality can be effectively improved, the operation process is simplified, and the experiment cost and the reagent loss are reduced.

The utility model relates to biological tissue sample microscopic imaging technical field provides a slice formula three -dimensional tomography optical microscopic system, it includes ultrathin section module, optical imaging module and data acquisition module, ultrathin section module is used for realizing to sample's scanning formula section, ultrathin section module includes processing groove, cutter assembly, processing liquid circulating device and flush device, cutter assembly has the upper surface of slanting upwards, and the tissue slice obtained after sample is sliced via cutter assembly is exported from the upper surface;The liquid inlet of processing liquid circulating device is set up in the section export direction corresponding the upper surface, and processing liquid circulating device is used for forming the water flow suction field on the upper surface;The water outlet of flush device corresponds the upper end surface of sample and is set up towards cutter assembly's direction, and flush device is used for forming the water flow impact force along section export direction on the upper end surface of sample and the upper surface, the accuracy and reliability of the system to biological sample three -dimensional structure information acquisition are improved.

The invention discloses a multiple immunofluorescence color development kit and application thereof, the multiple immunofluorescence color development kit comprises a fluorescent material, the structure of the fluorescent material is as shown in formula (I), the fluorescent material in the multiple immunofluorescence color development kit has a weak fluorescence signal in the absence of HRP enzyme catalysis, and does not generate non-specific adsorption staining on tissue slices, so that the problem of non-specific staining in the use process of existing immunofluorescence histochemistry can be solved.

The present invention discloses a staining method for co-staining of three fibrous tissues, which includes the steps of successively using Victoria blue staining and Gordon-Sweets silver ammonia solution staining on the processed tissue sections; the staining method of the present invention combines Victoria blue staining and silver ammonia solution staining, and can clearly present the staining of three fibrous tissues on the same section, clearly show the relationship between the cell nucleus structure and elastic fibers, significantly improve the diagnostic accuracy of the degree of tumor invasion, reduce the demand for tissue samples, and the staining method of the present invention has the advantages of clear color contrast, rich levels, accurate positioning, simple operation, etc., and has important significance for clinical pathological diagnosis and is worthy of popularization and application.