69 results about "Microdissection" patented technology

In a first aspect, a method for use in biology, histology, and pathology, comprises: providing a digital first image (44) of a first slice (12) of an object (10) comprising biological material; generating a digital second image (46) of a second slice (14) of the object; determining a region of interest (50) in the second image on the basis of a region of interest (48) in the first image; determining a region of interest in the second slice on the basis of the region of interest (50) in the second image; and extracting material from the region of interest in the second slice. In another aspect, an information processing system, for use in biology, histology and pathology, comprises: a predefined set of process identifiers (64); a set of data records (68, 70, 72) associated with an object (10) comprising biological material, wherein each of the data records comprises: a slice identifier identifying a slice (12; 14) of the object, and a process identifier selected from the set of process identifiers, the process identifier indicating a process to which the slice is intended to be subjected; a user interface (52, 56, 58, 60) for enabling a user to select a data record (68) from the set of data records.

A computer implemented method of generating at least one shape of a region of interest in a digital image is provided. The method includes obtaining, by an image processing engine, access to a digital tissue image of a biological sample; tiling, by the image processing engine, the digital tissue image into a collection of image patches; identifying, by the image processing engine, a set of target tissue patches from the collection of image patches as a function of pixel content within the collection of image patches; assigning, by the image processing engine, each target tissue patch of the set of target tissue patches an initial class probability score indicating a probability that the target tissue patch falls within a class of interest, the initial class probability score generated by a trained classifier executed on each target tissue patch; generating, by the image processing engine, a first set of tissue region seed patches by identifying target tissue patches having initial class probability scores that satisfy a first seed region criteria, the first set of tissue region seed patches comprising a subset of the set of target tissue patches; generating, by the image processing engine, a second set of tissue region seed patches by identifying target tissue patches having initial class probability scores that satisfy a second seed region criteria, the second set of tissue region seed patches comprising a subset of the set of target tissue patches; calculating, by the image processing engine, a region of interest score for each patch in the second set of tissue region seed patches as a function of initial class probability scores of neighboring patches of the second set of tissue region seed patches and a distance to patches within the first set of issue region seed patches; and generating, by the image processing engine, one or more region of interest shapes by grouping neighboring patches based on their region of interest scores.

A method of collecting target regions from a target object is described. The method in one embodiment comprises mounting a negatively-charged membrane on a first side of a substrate, mounting a target object on the membrane, positioning a collection material adjacent to the target object, and passing a laser beam from a second side of the substrate, through the substrate, the membrane, and the target object, to dissect target regions from the prepared tissue section, whereby the dissected target regions adhere to the collection material. In another embodiment, the present invention is a system for collecting target regions from a target object. In one embodiment, the system comprises a substrate having a first side and a second side, a negatively-charged membrane adhered to the first side of the substrate, and a collection material mountable adjacent to the membrane. In another embodiment, the system further comprises an inverted microscope, a stage for holding the substrate over the microscope, a generator operable to generate a laser beam to pass through the substrate from the second side and to dissect target regions from a target object mounted on the membrane, whereby the dissected target regions adhere to the collection material. In the preferred embodiments, the target objects are tissues and the target regions are cells.

A single optical fiber having a distal end is optically coupled to the laser and distilling terminated with an axicon lens optically coupled to the single optical fiber to form a microscopic distal tip to provide a spatially shaped elongated laser focused spot for microprocessing and/or microdissection of a microscale object. A pulsed or continuous laser beam or superposition of pulsed and continuous laser beams is generated, controllably spatially shaped, selectively oriented, selectively moved via movement of a single optical fiber terminated with the axicon lens, and the oriented, spatially shaped laser beam applied via the single optical fiber terminated with the axicon lens to a living or nonliving microscopic object for manipulation, micro-dissection, alteration/ablation, and excitation of the living or nonliving microscopic object.

A method of laser forward transfer is disclosed. Photo energy is directed through a photon-transparent support and absorbed by an interlayer coated thereon. The energized interlayer causes the transfer of specific regions of a heterogeneous tissue sample coated thereon across a gap and onto a receiving substrate or into a receiving vessel.

The invention discloses a collection device for collecting cells after laser microdisssection, a method and a system thereof. The device provided by the invention comprises a sample carrier, two sides of which are respectively provided with a transparent film layer and an insulating layer, wherein the transparent film layer fits with the sample carrier and is equipped with cell tissue samples, and a first electrode is disposed between the insulating layer and the sample carrier; a sample collector which is filled with a cell adhesion material for the adhesion of cell tissues and is also provided with a light-sensitive material for photoelectric reaction after laser irradiation; and a power supply device, the positive electrode of which forms break-make stations with the first electrode through a first switch. By the above scheme, the disadvantage that the cell tissue samples during the collection process are polluted and that requirements on materials of the sample carrier and the sample collector are high, carried static charges are inconvenient to preserve and the collection efficiency is poor in the prior art is avoided. According to the cell collection device and the method thereof, functions are stable, and cell tissue samples will not be damaged.

Methods for laser microdissection isolation of viable cells are provided. Cells of a desired type may be isolated from a diverse population, optionally with detection and exclusion of undesired cells. Desired cells may be isolated from a population that arose from differentiation of pluripotent cells, preferably embryonic stem cells or induced pluripotent stem cells, and undifferentiated stem cells may be detected and excluded from selection including the isolation of RPE cells sleeted based on morphology (e.g., characteristic mottled appearance) from a population of ES cells. The cells isolated by these methods, including RPE cells, may be essentially free of undifferentiated cells and thus suitable for use in cell-based therapies.

A laser microdissection unit for cutting a microscopic sample using a laser beam of a laser includes a microscope and a fluorescence device. The microscope includes an illumination beam path directed onto the sample, and an imaging beam path configured to image the sample. The fluorescence device includes an excitation filter, a dichroic beam splitter, and a blocking filter. The dichroic beam splitter and the blocking filter are spectrally transparent to the laser beam, and the laser beam is directable through the dichroic beam splitter and the blocking filter onto the sample.

The invention discloses technology for washing and recycling a membranate glass slide for microdissection. The technology can be implemented according to the following steps: (1) dipping and shaking the membranate glass slide in mixed solution A of boric acid, alcohol and EDTA; (2) washing the membranate by using double distilled water, and transferring the membranate glass slide into prolease K solution to incubate; (3) placing the glass slide into clean water; slightly touching a sample on the membrane by a finger; and washing the membranate glass slide by the double distilled water; (4) dipping the glass slide in mixed solution B of hydrochloric acid, the alcohol and the EDTA; washing the membranate glass slide by using the double distilled water and absolute ethyl alcohol; and finishing the last washing in the absolute ethyl alcohol; and (5) quickly transferring the membranate glass slide onto a super-clean bench; quickly drying the absolute ethyl alcohol; and carrying out ultraviolet radiation at the same time. The technology removes tissues, nucleic acid, albumen and other micromolecular substances remained on the surface of a consumptive material, and can sufficiently maintain the completeness and adhering effect of the membranate glass slide for microdissection.

The invention relates the microscopic cutting device. The invention overcomes the defects of strong working strength, fatigability and big personal error. The device comprises computer 1, microscope 2, CCD cam 3, free mechanical micro motion desk 4, clamp 5, ultrasonic vibration emitter 6, cutting needle 7, biological section stage 8 and cutter bar. 2 is installed under 8, 3 is connected with 2 to collect the object magnified by 2, the signal output terminal of 3 is connected with signal input terminal of 1, 4 is installed at the side of 8, the signal output terminal of 1 is connected with controlling terminal of 4, 5 is fixed on the 4, 11 is fixed on the 5, one end of 11 is connected with one end of 6, the other end of 6 is connected with the butt of 7, the tip of 7 extends to the upper surface of 8, and the other signal output terminal of 1 is connected with the input terminal of 6.

The invention relates to a laser microdissection system (100), comprising a microscope (10). The microscope comprises a reflected-light device (76), a microscope objective (41), and a laser unit (70). A beam path (b) of a laser beam of the laser unit (70) extends through the reflected-light device (76) and through the microscope objective (41) and intersects with an object plane (15) of the microscope objective (41) at an adjustable point of intersection. An electrophoresis unit (90) having an electrophoresis gel (91) is attached, or can be attached by means of coupling elements (35), below the object plane (15), wherein the electrophoresis gel (91) has at least one gel pocket (92) and the laser microdissection system (100) comprises positioning means (96) for positioning the electrophoresis gel (91) in parallel with the object plane (15) and in relation to a defined reference position, such that dissectates (99) of a sample (51) that can be arranged in the object plane (15), which dissectates are obtained by means of the laser beam of the laser unit (70), can be captured in the at least one gel pocket (92). The invention further relates to a corresponding electrophoresis unit (90) and an examination method.

The invention relates to a chromosome specimen preparation liquid for laser microdissection and application thereof. The specimen preparation liquid comprises methanol, glacial acetic acid and chloroform of which the volume ratio is (5-10):(1-3):(1-3), preferably 8:1:1. The chromosome specimen preparation liquid provided by the invention is used for preparing a chromosome specimen for laser microdissection. In the chromosome specimen for laser microdissection, which is prepared by the chromosome specimen preparation liquid, the chromosomes are well dispersed, thereby facilitating the subsequent microdissection operation. Meanwhile, the preparation method provided by the invention is simple and practical, is easy to operate, and is convenient for common technical personnel to use.

The invention relates to a multi-PCR detection method of trace DNA, which can avoid the competition inhibition problem due to the excessive primers of the multi-PCR technology by changing the concentration and the adding mode of the primers, so the multi-PCR detection method can be used for the very limited DNA of an initial sample, in particular to a plurality of very valuable clinical samples, such as, fertilized eggs and the obtained cells by laser capture and microdissection, etc. The whole detection proposal of the invention has simple operation process, high specificity, very high sensitivity and PCR efficiency, easy automation and wide application prospect in practical application.

The invention provides a dripping piece preparation method of plant chromosome. The dripping piece preparation method includes steps of placing a plant organ in nitrous oxide gas for pretreatment, and then fixing the plant organ after pretreatment by stationary liquid, and performing enzymolysis on a cell wall of the plant organ by enzyme, and breaking the plant organ after enzymolysis, and preparing the cell suspension liquid; dripping the cell suspension liquid on a glass slide or a cover glass; forming a plant chromosome specimen. The reagent applied to the method is low in toxicity; the crack obtained through treatment is dispersed in a circular or ellipse form by comparing with regular piece making method, the distribution is uniform, and chromosome intersection is few, and dispersing degree is good; a complete chromosome can be obtained; the preparation method can prepare the chromosome slice with stable quality, clear chromosome and good dispersity; the prepared chromosome slice can be applied to cytological identification, manufacturing of permanent slice, hybridization in-situ, microdissection, and microscope separation, and so on.

The invention provides a method for evaluating physical and mechanical properties of wood based on microscopic morphological characteristics of wood, which is characterized by comprising the followingsteps: 1, sampling: selecting wood at the bottom end of a to-be-analyzed wood section; 2, testing: testing the micromorphological characteristics of the selected wood; 3, evaluation: establishing a regression model for the bending strength, the bending elasticity modulus and the tensile strength of the wood according to the tested data, and evaluating the parameters according to the regression model. The invention provides a method for evaluating the physical and mechanical properties of wood based on the micromorphological characteristics of wood wherein stepwise regression analysis is carried out by taking the bending strength, the bending elasticity modulus and the microsection tensile strength of China fir as dependent variables and microdissection indexes as independent variables, and a regression equation is established; therefore, the method can predict the bending strength, the bending elasticity modulus and the tensile strength of the cunninghamia lanceolata, provides a theoretical basis for reasonable processing and utilization of wood, directional cultivation of forest trees and the like, and has important significance for improving the utilization rate of the wood.

About 70% to 80% of breast cancers express estrogen receptor-α (ERα), and estrogens play important roles in the development and growth of hormone-dependent tumors. Together with lymph node metastasis, tumor size and histological grade, ER status is considered one of the prognostic factors in breast cancer, and an indicator for hormonal treatment. 147 genes and 112 genes with significant P-value and having significant differential expression between ER+ and ER− tumors were identified from the LCM data set and bulk tissue data set, respectively. 61 genes were found to be common in both data sets, while 85 genes were unique to the LCM data set and 51 genes were present only in the bulk tumor data set. Pathway analysis with the 85 genes using Gene Ontology suggested that genes involved in endocytosis, ceramide generation, Ras/ERK/Ark cascade, and JAT-STAT pathway may play roles related to ER. The gene profiling with LCM-captured tumor cells provides a unique approach to characterize and study epithelial tumor cells and to gain an insight into signaling pathways associated with ER.

The invention provides an identification method of nucleated cells and an application thereof, the identification method of the nucleated cells can be applied in a single-cell separation detection platform with the combination of the micromanipulator capture method and LV-PCR and the single-cell separation detection platform with the combination of the laser microdissection method and the LV-PCR,and the method comprises the following steps: placing a sample containing the nucleated cells in a centrifugal tube containing buffer solution, oscillating the centrifugal tube to lead the cells on the sample to fall off, and obtaining cell suspension of the cells of the sample; and adding gentian violet or haematoxylin into the cell suspension for dyeing, wherein the final concentration of the gentian violet is 0.1-0.5 mu g/mu l, the dyeing time is 2-7 minutes, the final concentration of the haematoxylin is 1-7 mu g/mu l, and the dyeing time is 3-7 minutes. The use of the cell identificationmethod in the two single-cell separation detection platforms can realize effective identification of the cells of the sample, lead the follow-up LV-PCR amplification inhibition to be weak and obtain good DNA typing result.