69 results about "Haematoxylin" patented technology

A green making technology of animal tissue paraffin section disclosed in the invention aims at providing a technique of making a paraffin section of animal tissue applied to the agricultural university medicine and other majors, characterized by that the type of chemical reagents that are used is limited, the chemical reagents have no toxicity, and dehydrate the tissue thoroughly, accelerate making sections but not lead to excessive contraction and increasement of hardness of the tissue, and substitute dimethyl benzene used in the traditional making technology of the paraffin section which isharmful to human body, so as to eliminate the occupational diseases and environmental pollution caused by dimethyl benzene. The making technology comprises the following steps: carrying out fixation with a fixative solution (100ml of the fixative solution comprises 91 ml 85% alcohol, 4 ml methanol and 5 ml glacial acetic acid ), dehydrating the tissue with graded ethanol, then putting the fixative and dehydrated tissue in a mixed transparent reagent (comprising 14wt% of analytically pour n-butanol, 29wt% of acetone and 57wt% of absolute alcohol ) for standing for 3 hours, then carrying out normal waxing and embedding, using analytically pour turpentine (60 DEG C) for section staining, carrying out dewaxing twice, carrying out hydration under normal temperature, using haematoxylin for staining nuclei, decoloration and bluing, and using eosin for staining, dehydrating until get absolute alcohol, then carrying out air drying, and directly using the gum diluted by turpentine to sealing the section.

The invention provides a medical tissue section staining method which comprises the following steps that: olefin is sliced into sheets, dewaxed and hydrated; sliced sheets are put into solution of picric acid, formaldehyde and glacial acetic acid after being heated, taken out and washed by running water; the sliced sheets are put into a 5 percent sodium thiosulfate solution to soak and washed by distilled water; the sliced sheets are put into an alcian blue solution to soak and washed by the running water; the sliced sheets are put into a preheated alkaline ethanol solution to soak and washed by the running water; under the lighttight condition, the sliced sheets are put into a haematoxylin working solution and washed by the running water and the distilled water; the sliced sheets are put into a saffron/ acid fuchsine working solution and washed by the distilled water; the sliced sheets are put into a phosphotungstic acid solution to soaked, then transferred to the glacial acetic acid to soak and washed by the distilled water; the sliced sheets are dehydrated by ethanol; the sliced sheets are put into an ethanol saffron solution to soak, then dehydrated by the ethanol and sealed. The medical tissue section staining method has low cost and vivid staining, shortens the time for a staining flow and also increases the application range.

The invention discloses a preparation method and an application of a tissue slice for observing temporal-spatial distribution of early embryo development in vivo. The preparation method comprises the following steps that 4% paraformaldehyde fixing, upward gradient ethanol dehydration, wax dipping, embedding, serial section, baking, dewaxing and downward gradient ethanol rehydration are performed in sequence on oviducts or uterine tissues which contain mice embryos in every period, and finally, after haematoxylin-eosin staining is performed on the tissues in the slice, neutral gum is used for sealing the slice, or after immunofluorescence histochemical staining is performed on the slice, a fluorescence resistant quenching sealing agent is used for sealing the slice. The tissue slice disclosed by the invention can be used for manufacturing a map of early mice embryo development and detecting the expression of Crb3 in the mice embryos in every period of development in vivo. The preparation method has the advantages that positions of all organs in the embryos can be relatively fixed, so that the position change of embryo cells in a genital tract and the continuity of embryo development can be conveniently observed, the structure is clear, and the tissue slice is convenient to store.

The invention discloses a hematoxylin-eosin staining pathological image segmentation method based on unsupervised deep learning, and the method comprises the steps: carrying out the preprocessing andfeature extraction after an HE staining pathological image is obtained, dividing pixels into five types through Kmeans clustering, using a given training sample full-automatic grabbing strategy for carrying out traversal grabbing on category label images obtained through clustering to obtain reliable training samples, then using a training set for training a semantic segmentation model Unet, and designing different training strategies before, in the middle and after training; segmenting the to-be-segmented image into the size conforming to model input in an overlapping manner, putting the to-be-segmented image into the trained model to obtain a prediction result, and splicing the prediction result to obtain a segmentation result of the cell nucleus foreground; and finally, carrying out accurate kernel boundary segmentation on the cell nucleus part by using a hybrid watershed segmentation method to obtain a complete segmentation result. According to the method, the high efficiency of unsupervised learning and the high precision of deep learning are organically combined, and the precision and the efficiency of cell nucleus region segmentation in a pathological image segmentation taskare remarkably improved.

The invention discloses a deep learning-based intelligent detection method for nuclear division images in gastrointestinal stromal tumor. The method comprises the following steps: preprocessing an obtained hematoxylin-eosin staining pathological image; taking EfficientDet-D0 as a deep learning detection model, and carrying out training; using U-Net as a deep learning segmentation model, and training the deep learning segmentation model; constructing a deep learning classification model; training the deep learning classification model; detecting the hematoxylin-eosin staining pathological imageof the testee by using the trained deep learning detection model; segmenting the pathological image by using a deep learning segmentation model, and detecting the segmented result; and comparing thenuclear division images detection result based on the deep learning detection model with the nuclear division images detection result based on the deep learning segmentation model to obtain a final classification result. According to the invention, the input hematoxylin-eosin staining image is analyzed, and the number of nuclear division images is detected, so that the judgment on the risk degreeof gastrointestinal stromal tumor is realized.

The invention discloses a preparation method of a hyriopsis cumingii pallium tissue slice. The preparation method comprises the following steps of: carrying out fixation, upstream gradient ethanol dehydration, vifrification, waxdip, embedding, sectioning, baking, dewaxing and downstream gradient ethanol rehydration on pallium tissue on cumingii pallium, and mounting on the slice tissue by adopting neutral resins after carrying out hematoxylin-eosin on the slice tissue. The tissue slice prepared by the invention can be used for observing the histology characteristic of the pallium, and detecting expression of related genes forming the shell on the positions of the hyriopsis cumingii pallium; the method provided by the invention can guarantee the relative stationarity between positions of each layers of tissues of the pallium and the cell; and compared with the common preparation method of the tissue slice, the preparation method provided by the invention has the advantages that time is saved, the problem of tissue fracture of the tissue slice for the pallium manufactured by the common method is overcome, and the repeatability is better.

The present invention relates to methods of visualizing targets in histological samples, e.g. biopsy samples, wherein the methods comprise staining of the sample with (i) one or more target specific immunochemical stains, and (ii) a histological stain for specific tissue components e.g. iron, mucins glycogen, amyloid, nucleic acids, etc., e.g. hematoxilyn and/or eosin stains or the like, that is used to enhance contrast in the microscopic image of a tissue sample, highlight morphologic structures in the sample for viewing, define and examine tissues, cell populations, or organelles within individual cells. Methods may further comprise evaluation of expression of one or more targets in the sample. The disclosed methods are useful for medical diagnostics.

An electrochemical sensor capable of simultaneously measuring contents of hematoxylin and brazilin is disclosed, which is characterized in that CV (coefficient of variation) curves in hematoxylin with different concentrations and brazilin with different concentrations are measured in a buffering solution with the pH value of 3-11 by using a three-electrode system with a poly(p-aminobenzene sulfonic acid)/platinum nanometer wire-carbon nanotube composite-film modified electrode as a working electrode, wherein the temperature is 20-80 DEG C, and the scanning speed is 30-600mv/s, so as to obtain a linear response relationship, lower detectable limit and the like. The electrochemical sensor can simultaneously measure the contents of the hematoxylin and the brazilin.

The invention relates to a hematoxylin-eosin staining pathological image hierarchical segmentation method. The method comprises steps of obtaining a pathological image according to color intensity information of pixels in the pathological image; carrying out preprocessing and feature selection on the original image; gradually carrying out segmentation of three levels of K-means clustering, naive Bayesian classification and watershed segmentation, obtaining the precise boundary between the cell nucleuses, wherein accurate boundary segmentation of cell nucleuses in pathological images is a difficult point in pathological image segmentation, and the method significantly improves the segmentation precision of cell nucleus boundaries of hematoxylin-eosin staining pathological images while ensuring correct segmentation of regions, thereby improving the accuracy of cell nucleus counting results and morphological feature measurement.

The invention discloses a method for extracting residual nucleic acid from an HE (haematoxylin eosin) dyeing piece, and the method comprises the following steps of: decoloring the HE dyeing piece by using low concentration hydrochloric acid alcohol as a decoloring agent first, then, bleaching by an oxalate solution, and then extracting nucleic acid by enriching target cells. Compared with the prior art, the method provided by the invention can be used for effectively removing basic dye haematoxylin and acidic dye eosin, reducing the degradation of nucleic acid in the extracting and storing process effectively, and enriching and detecting needed target cells so as to extract residual nucleic acid by using all regular HE dyeing pieces such as a puncture tissue, a cell smear (including sputum and urea), gastrointestinal endoscope and the like and perform subsequent molecular neuropathologic detection, so that the method provided by the invention is good in practicality, and can be used for achieving good economical benefit and social benefit.

The invention provides a zooplankton microscopic slide specimen preparation technology. The zooplankton microscopic slide specimen preparation method comprises the following steps: with a concave slide as a carrier, carrying out formalin fixation, haematoxylin dyeing, acid alcohol color separation, tap water bluing, alcohol dewatering, glycerin hyalinizing, and neutral balsam sealing, thereby preparing a zooplankton microscopic slide specimen. The long-time storage of zooplankton materials is realized, the morphological structures of the materials can be kept maximally, and the observation clarity and contrast of the specimen can be improved.

The invention discloses a three-dimensional dyeing method for amygdalus communis l anther cells. According to the three-dimensional dyeing method, an amygdalus communis l anther slice treated with a conventional paraffin slicing method is dyed with an iron haematoxylin solution prepared 10 min in advance, such that the pollen granulocytes, the tapetal cells, the intermediate cells, the connective cells, the endothecium cells, the epidermic cells, the cells at the connection tissue position and the filaments of the amygdalus communis l anther can be clearly colored, and the coloring degrees among the cells at different parts are different so as to provide the three-dimensional dyeing effect. The three-dimensional dyeing method can be used for the study on the three-dimensional dyeing method internal structure change and the anther development process.

The invention discloses a method for preparing a smooth muscle cell carrier based on a small intestine submucosa acellular matrix. Vagina smooth muscle cells are successfully cultured in vitro, and the cultured vagina smooth muscle cells are seen to be in a long fusiform and gathered on a culture dish to form a typical peak and valley structure when invertedly placed under a microscope. The submucosa acellular matrix is white in appearance and translucent and has certain tenacity. Hematoxylin-eosin dying is conducted, and no cell component exists. After a vagina smooth muscle cell-intestine submucosa specimen section is dyed by hematoxylin-eosin, the number of visible cells under a light microscope is gradually increased, and the visible cells grow from the surface to the deep layer. After the vagina smooth muscle cell-intestine submucosa specimen section is dyed by an anti-rabbit smooth muscle alpha-actin monoclonal antibody in an immunohistochemical mode, positive cells of anti-rabbit alpha-Actin can be seen. The result preliminarily proves that the pig intestine submucosa acellular matrix can be taken as the smooth muscle cell carrier.

The invention discloses natural healthy hair dye containing hematoxylin and catechin. The natural healthy hair dye comprises 60-65% of hematoxylin, 30-35% of catechin and 5-10% of water-soluble ferrous salt. An extraction method of hematoxylin includes: smashing dry hematoxylon, and disposing smashed dry hematoxylon in a container; adding ultra-pure water according to a material-liquid ratio of hematoxylon to ultra-pure water of 1:30; adding sodium hydroxide into the container to adjust pH value of material liquid to 11; water-bath stirring and heating the container at 100 DEG C for 65 min; cooling the material liquid, and using filter paper to filter out impurities to obtain hematoxylin. An extraction method of catechin includes: smashing dry catechu, and disposing smashed dry catechu in a container; adding ultra-pure water according to a material-liquid ratio of catechu to ultra-pure water of 1:40; adding sodium hydroxide into the container to adjust pH value of material liquid to 11; water-bath stirring and heating the container at 90 DEG C for 45 min; cooling the material liquid, and using filter paper to filter out impurities to obtain catechin. The natural healthy hair dye is unitary in ingredient and good in hair dyeing effect.

The invention discloses application of ginsenoside Rg3 in combination with GP as an antitumor drug. A mouse Lewis lung cancer cell line (LLC) is selected and inoculated to the right armpit of a C57BL/6N mouse, and the influence of ginsenoside Rg3 and Rg3 in combination with GP on Lewis lung cancer is observed; hematoxylin-eosin staining is performed on the liver, kidney, spleen and tumors, and pathological changes of ginsenoside Rg3 in combination with GP on the liver, kidney, spleen and tumors of mice with the Lewis lung cancer are detected; and tumor tissues are subjected to immunohistochemical staining, and the density of blood vessels in tumors and the expression of tumor platelet surface activation markers are detected. The conclusion is that the ginsenoside Rg3 in combination with GPcan enhance the effects of ginsenoside Rg3 of inhibiting the tumor growth of the mice with the Lewis lung cancer and inhibiting tumor angiogenesis, and the tumor inhibition effect of the ginsenosideRg3 is possibly and closely related to CD62P expression reduction; and the ginsenoside Rg3 in combination with GP can achieve the synergistic and toxicity-reducing effects.

The invention relates to a rapid cast-off cell sample dyeing agent and a preparation method thereof. The rapid cast-off cell sample dyeing agent comprises the following components in parts by weight: 3.5 parts of haematoxylin, 45 parts of absolute ethyl alcohol, 50 parts of aluminum potassium sulfate dodecahydrate, 500 parts of distilled water, 2 parts of mercuric oxide and 6 parts of glacial acetic acid. The preparation method of the rapid cast-off cell sample dyeing agent comprises the following steps: firstly putting aluminum potassium sulfate dodecahydrate into distilled water, heating to about 80 DEG C for dissolving aluminum potassium sulfate dodecahydrate, and cooling to normal temperature, so that an aluminum potassium sulfate dodecahydrate dissolved solution is obtained; and adding haematoxylin into absolute ethyl alcohol, dissolving, mixing with the aluminum potassium sulfate dodecahydrate dissolved solution, and then sequentially adding mercuric oxide and glacial acetic acid, so that the rapid cast-off cell sample dyeing agent is obtained. A cast-off cell smear sample is obtained by dyeing for 30+/-s by utilizing the rapid cast-off cell sample dyeing agent and washing for 10+/-s with flowing water. Compared with the prior art, the rapid cast-off cell sample dyeing agent has the beneficial effects that the dyeing time is shortened to 1 minute from 1 hour, the clinical diagnosis time is much earlier, a method is simple and easy to learn, and the cost of used raw materials is low; meanwhile, the time consumed by inspection personnel is less, the labour cost is low, and the financial burden of a patient is low.

The invention discloses a method for detecting OGN protein expression and application thereof in gastric cancer auxiliary diagnosis. The method comprises the following steps: taking pathological tissues of a patient with gastric cancer, carrying out paraffin embedding slicing, and dewaxing paraffin sections to water; primarily treating the dewaxed slices, soaking the dewaxed slices in a hydrogen peroxide solution in a dark place, and rinsing the dewaxed slices with pure water; repairing with a citric acid buffer solution and a microwave antigen, cooling to room temperature, rinsing with pure water, putting the slices into a wet box, performing immunohistochemical stroke circle marking, dropwise adding serum derived from a secondary antibody, and sealing; incubating the primary antibody, the secondary antibody and the SABC; carrying out light re-dyeing, dehydration and transparency on hematoxylin, carrying out microscopic examination after sealing, and taking brown yellow or light yellow particles appearing on cytoplasm or cytomembrane as positive expression. The expression condition of OGN in gastric cancer tissue can be detected through immunohistochemical staining of pathologicaltissue of a gastric cancer patient, the method is easy and convenient to operate and accurate in detection, and a basis is provided for diagnosis, treatment and evaluation of the gastric cancer patient.

The invention discloses a staining method using fluorescence quenching time difference. The staining method comprises the following steps of: dewaxing a histopathological section to obtain a section to be treated, and carrying out fluorescence staining on the section to be treated at least once by using a fluorescence staining reagent with a preset antibody to obtain fluorescence staining sectionscomprising preset fluorescence labels; quenching the preset fluorescence labels generated in the previous fluorescence staining before carrying out the next fluorescence staining; and scanning each fluorescence staining section to obtain a fluorescence image; quenching the preset fluorescence labels, coloring and staining the section to be treated by using a hematoxylin eosin staining method to obtain a colored and stained section, and scanning the colored and stained section to obtain a colored image; and performing image superposition fusion processing on the at least one fluorescence imageand the colored image to obtain a cross-staining fusion image. Therefore, the staining method can meet requirements of the stained image on the observation range and the staining specificity. In addition, the invention further provides a staining device and staining equipment using the fluorescence quenching time difference, and a medium.