23 results about "Intermediate cell" patented technology

The invention relates to the technical field of photovoltaic, and provides a photovoltaic module and a manufacturing method thereof, which are at least beneficial to improving the efficiency and yield of the photovoltaic module. The photovoltaic module comprises at least two battery strings arranged along a second direction, each battery string comprises a plurality of back contact batteries which are sequentially arranged along a first direction, the first back contact battery and the tail back contact battery in the battery strings are end batteries, and the back contact battery between the end batteries is an intermediate battery; the two ends of the first welding strip are located on the surfaces of the two adjacent back contact batteries in the first direction respectively; the first covering film covers the surface of the middle battery; the second welding strip is positioned on the surface of the end battery; the second covering film covers the surface of the end battery; the first bus bars are located on the middle battery and located on the side, away from the back contact battery, of the first covering film, and the multiple second welding strips are welded to the same first bus bar; and the end parts of the first bus bars of the two battery strings are electrically connected through welding.

The invention relates to an inter-cell cooling device (10) for a battery (12) with a cell stack (18) comprising several battery cells (20) arranged side by side in a stacking direction (S), wherein the inter-cell cooling device (10) comprises an inter-cell cooling unit (14) for arrangement between at least two adjacent battery cells (20) of the battery cells (20) of the cell stack (18), wherein the inter-cell cooling unit (14) has a phase change material (32), a first interior space (28) and a first outer shell (26) enclosing the first interior space (28).The intercellular cooling unit (14) comprises at least one thermal insulation cushion (34) arranged entirely in the first interior space (28), which has a second interior space (38) and a second outer shell (36) enclosing the second interior space (38), wherein the phase change material (32) is arranged in the first interior space (28) outside the at least one thermal insulation cushion (34), and the at least one thermal insulation cushion (34) comprises a fluid (40) arranged in the second interior space (38).

The present invention relates to a method, preferably a computer-implemented method, and to an apparatus for generating at least one intermediate cellular state of a cellular state evolution from an initial cellular state to a corresponding advanced cellular state. The present invention also relates to a computer program for generating at least one intermediate cellular state and to a non-transitory computer readable data medium storing the computer program. Moreover, the present invention relates to a method and to an apparatus for training a model for generating at least one intermediate cellular state of a cellular state evolution from an initial cellular state to a corresponding advanced cellular state. The present invention also relates to a computer program for training a model for generating at least one intermediate cellular state and to a non-transitory computer readable data medium storing the computer program. Furthermore, the present invention relates to a method for diagnosing a disease and forecasting patients' future clinical outcome making use of the method for generating at least one intermediate cellular state. Moreover, the present invention relates to an in-vitro method for transforming the identity of a cell.

The invention belongs to the technical field of biological material preparation, and particularly relates to a preparation method of a self-oxygen-supply hydrogel cell scaffold, an obtained product and application. According to the invention, stable and continuous release of oxygen and three-dimensional (3D) culture of neural stem cell spheres are realized. The method is realized by the following steps: adding compounds P-MA, P-Cys, ColMA and PEG-CaO2 into a PBS (Phosphate Buffer Solution), adding a photoinitiator, and irradiating and curing by ultraviolet light with the wavelength of 365nm to obtain the self-oxygen-supply composite hydrogel. The invention provides a novel method for applying composite hydrogel loaded with oxygen-producing nano-particles to 3D cell sphere culture and continuously releasing O2 in a slightly acidic environment to solve the problems of death of intermediate cells caused by oxygen deficit in the 3D culture process of existing cell spheres, indefinite components of matrigel, high price and the like. The problem of internal oxygen deficit in the cell ball culture process is solved, and the gel component is defined to be used for researching the effect between other components and cells. In addition, the self-oxygen-supply hydrogel material prepared by the technology is expected to be applied to 3D culture of other cell spheres or organoid spheres.

The invention relates to an intermediate cell cooling device (10) for arrangement in a gap (16) between two cells (14) arranged adjacent to each other in a stacking direction (x), comprising: a first side wall (18) having a first outer side (18a) for arrangement on a first cell side (14b); a second side wall (20) opposite the first side wall in the first direction and having a second outer side surface (20a) for arrangement on a second cell side surface (14a); and a heating device (24) designed as a heating layer (24) for heating the intermediate cell cooling device. At least one cooling channel (30a, 30b) through which a coolant can flow in the first direction between the first side wall and the second side wall is designed as part of a central intermediate cell cooling region (12) of the intermediate cell cooling device, and the heating layer is designed to heat the coolant (32) located in the at least one cooling channel.

The invention relates to the technical field of cell culture, in particular to nano liver stem cell peptide as well as a preparation method and application thereof. The preparation method of the nano liver stem cell peptide comprises the following steps: (1) mixing induced pluripotent stem cells with a first induced differentiation culture medium, and culturing for 3-5 days to obtain an intermediate cell 1; (2) inoculating the intermediate cell 1 into a second induced differentiation culture medium, and culturing for 3-5 days to obtain an intermediate cell 2; and (3) inoculating the intermediate cell 2 into a third induced differentiation culture medium, and culturing for 3-5 days to obtain the liver stem cell, and (4) after the liver stem cells are subjected to ultrafiltration, taking substances of which the molecular weight cutoff is less than or equal to 50kDa, centrifuging, taking precipitates, and drying to obtain the liver stem cell peptide. The liver stem cell peptide prepared by the method has the particle size of 1-20nm, can target the liver, has a good effect in treatment of liver injury, and does not generate drug resistance.

To provide a method for producing a sheet-like cell culture showing a three-layer structure including a cell layer of superficial cells, a cell layer of intermediate cells, and a cell layer of basal cells, in this order. Provided is a method for producing a sheet-like cell culture that includes culturing a progenitor cell population in a second compartment separated from a first compartment via a porous member to form a sheet-like cell culture in the second compartment. The sheet-like cell culture includes a cell layer of superficial cells derived from the progenitor cell population, a cell layer of intermediate cells derived from the progenitor cell population, and a cell layer of basal cells derived from the progenitor cell population, in this order. The first compartment holds an induction medium containing retinoic acid, bone morphogenetic protein (BMP), and fibroblast growth factor (FGF).

The present application belongs to the technical field of biomaterial preparation, and particularly relates to a preparation method of a self-oxygen-supplying hydrogel cell scaffold, the obtained product and application. The present application realizes stable and continuous release of oxygen and three-dimensional (3D) culture of neural stem cell spheres. The method is realized through the following steps: compounds P-MA, P-Cys, ColMA and PEG-CaO2 are added to a PBS solution, a photoinitiator is added, and a'self-oxygen-supplying' composite hydrogel is obtained by irradiation with ultraviolet light of a wavelength of 365 nm. The present application provides a composite hydrogel loaded with oxygen-producing nanoparticles for 3D cell sphere culture and a new method for continuous release of O2 in a slightly acidic environment, aiming at the problems of intermediate cell death caused by hypoxia in the process of 3D culture of existing cell spheres, unclear matrigel composition, high price and the like. The present application solves the problem of internal hypoxia in the process of cell sphere culture, and clearly defines the gel composition for studying the interaction between other components and cells. In addition, the self-oxygen-supplying hydrogel material prepared by the technology is expected to be applied to 3D culture of other cell spheres or organoid spheres.

The invention relates to a heterojunction cell structure and a preparation method thereof. The heterojunction cell structure comprises an intermediate cell structure which is N-type monocrystalline silicon; the front cell structure comprises a front intrinsic amorphous silicon film and N-type amorphous silicon, the front intrinsic amorphous silicon film is stacked on one side of the middle cell structure, and the N-type amorphous silicon is stacked on one side, away from the middle cell structure, of the front intrinsic amorphous silicon film; the back cell structure comprises a back intrinsic amorphous silicon film and P-type amorphous silicon, the back intrinsic amorphous silicon film is stacked on the other side of the middle cell structure, and the P-type amorphous silicon is stacked on the side, away from the middle cell structure, of the back intrinsic amorphous silicon film; the thickness of the front intrinsic amorphous silicon film and the thickness of the back intrinsic amorphous silicon film are 5-10 nm, the thickness of the N-type amorphous silicon and the thickness of the p-type amorphous silicon are 15-20 nm, and the technical problem that the production cost is increased when a PECVD film making machine and vacuum equipment are used is solved.

The present invention relates to a cell intermediate element (2) with gas-filled or evacuated internal cavities (14) and to a method for producing such a cell intermediate element (2). The invention particularly relates to sealing the cavities (14) against the environment. The cell intermediate element (2) comprises: - a first covering layer (3), - a second covering layer (4), - an elastically deformable elastomer intermediate layer formed between the first cover layer (3) and the second cover layer (4). The intermediate cell element (2) comprises an outer shell made of a flexible film or a coating (12) is applied to seal the cavities (14) against the environment.

The present application discloses a single-welded cell and its preparation method, a cell string, and a photovoltaic module, relating to the technical field of solar cells. The preparation method comprises: providing a cell, the cell comprising a substrate and a conductive layer bonded to at least one surface of the substrate; heating the cell to a temperature T1, maintaining the temperature for a period of time t, and then cooling the temperature to a temperature T2 to obtain an intermediate cell; and placing a soldering tape on the conductive layer of the intermediate cell to obtain a single-welded cell. The heating and cooling processes provide the cell with a buffer period to adapt to temperature T and room temperature. The combined effects of the heating, maintaining, and cooling processes facilitate the full volatilization of moisture from the cell, reduce the internal stress of the conductive layer, prevent oxidation of the conductive layer, enhance the strong bonding between the substrate and the conductive layer, and thereby enhance the welding tension of the conductive layer grid lines on the single-welded cell; and then performing single welding with the soldering tape to prepare for the series connection of the cell and enhance the conductivity of the battery.

A process for anaerobically treating a feed of manure that contains liquid and suspended solid in a reaction that uses a multicell lagoon reactor (MCLR) wherein the reaction system has an input cell, an output cell, and one or more intermediate cell all in series and located at least partially below the ground. A partition separates the cells to restrict a gravity induced flow of liquid and solids retained cell. The solids retention time exceeds the hydraulic retention time in each cell. Enclosing the cells enables collecting of biogas from the cells. At least an input cell operates as an anaerobic sequencing batch reactor that undergoes periodic mixing. An output cell provides treated liquid for recovery.

The application discloses a novel dye-sensitized solar cell optimization method, and relates to the technical field of semiconductor devices, and comprises the following steps: on the basis of a dye-sensitized solar cell model, first, the material of a hole transport layer of the dye-sensitized solar cell model is changed, a plurality of first cell models are obtained correspondingly, and performance simulation is performed; based on the performance simulation result, an intermediate cell model is selected from the plurality of first cell models; then, the electrode material of the intermediate cell model is changed, a plurality of second cell models are obtained correspondingly, and performance simulation is performed; based on the performance simulation result, a to-be-adjusted cell model is selected from the plurality of second cell models; finally, a plurality of adjustable parameters of the to-be-adjusted cell model are optimized, and a target cell model is obtained. Through optimization of the material of the hole transport layer, the material of the electrode and the numerical value of part of the adjustable parameters of the dye-sensitized solar cell model, the performance of a solar cell corresponding to the dye-sensitized solar cell model can be improved.

The invention discloses a preparation method and application of renal progenitor cells. The method specifically comprises the following steps: inducing pluripotent stem cells to be differentiated into an intermediate mesoderm cell stage through a GSK-3alpha / beta inhibitor, and inducing cells in the intermediate mesoderm cell stage to be differentiated into a cap-like mesenchymal stage by using a nuclear receptor agonist containing FGF-2 and RAR / RXR, so as to obtain the renal progenitor cells with obvious specific characteristics and potentials of the cap-like mesenchymal. According to the method, the high-purity and high-quality renal progenitor cells can be obtained without separation and purification, the purity can reach 99.9%, and the obtained renal progenitor cells have the differentiation potential of kidney-like organs, basically have no PSCs residues, have the differentiation potential of kidney-like organs and are suitable for clinical cell therapy; and the operation is simple, stable and controllable, and safe, effective, high-purity and homogeneous renal progenitor cells can be prepared on a large scale.

An in vitro method of producing a population of cells comprising the steps of introducing a source of cells into the first cell culture chamber of an in vitro cell culture vessel comprising three cell culture chambers arranged in a series, each chamber being separated from the next chamber in the series by a semipermeable barrier; contacting the cells in the first cell culture chamber to a first composition of one or more cell growth factors, cell activating factors, cell differentiation factors, cytokines and cell attractants so as to promote migration of at least a subset of cells from the first cell culture chamber into an intermediate cell culture chamber of the series; and contacting cells in the intermediate cell culture chamber of the series to a further composition of one or more cell growth factors, cell activating factors, cell differentiation factors, cytokines and cell attractants so as to promote migration of at least a subset of cells from the intermediate cell culture chamber to the final cell culture chambers in the series. Also related cell culture vessels, cell populations and methods of using cell populations.

The invention relates to the technical field of cell culture, in particular to hepatic stem cells obtained by directional induced differentiation of mesenchymal stem cells as well as a preparation method and application of the hepatic stem cells. The induction method comprises the following steps: (1) inoculating mesenchymal stem cells into an induction culture medium, and culturing for 5-7 days to obtain intermediate cells 1; (2) inoculating the intermediate cell 1 into a differential medium, and culturing for 3-6 days to obtain an intermediate cell 2; and (3) inoculating the intermediate cell 2 into a mature culture medium, culturing for 3-6 days, filtering, centrifuging, collecting precipitate, and drying to obtain the liver stem cell. According to the method disclosed by the invention, the mesenchymal stem cells can be directionally converted into the liver stem cells. The liver stem cell obtained by the invention can be used for treating liver cirrhosis, has a good curative effect and no side effect, and provides a basis for treating liver cirrhosis.

Cell block (1) for a battery (2), comprising a plurality of electrochemical individual cells (3) electrically connected in series and / or parallel, which are designed as flat cells, are aligned parallel to each other and arranged one behind the other in the cell block (1) and are clamped together by means of at least one clamping element (11), wherein an intermediate cell holder (9) made of an electrically insulating material is arranged between each pair of adjacent individual cells (3), which has a partition (22) that runs between the respective two adjacent individual cells (3), and wherein a temperature control plate (16) is arranged on at least one longitudinal side of the cell block (1), with which the individual cells (3) are thermally coupled.wherein a cross-section of the partitions (22) of the intermediate cell holders (9) in an edge region (28) facing the temperature control plate (16) is enlarged compared to a cross-section in a central region (29) of the respective partition (22), with at least one end cell holder (10) made of an electrically insulating material at an end face of the cell block (1), wherein the end cell holder (10) has a partition (22) which covers an adjacent single cell (3), wherein a cross-section of the partition (22) in an edge region (28) facing the temperature control plate (16) is enlarged compared to a cross-section in a central region (29) of the respective partition (22), wherein this cross-sectional enlargement extends in the direction of the adjacent single cell (3), characterized in thatthat the intermediate cell holders (9) have side edges (23) on the edges running perpendicular to the edge region (28) facing the temperature plate (16), which each project beyond half of the adjacent individual cells (3), so that the respective side edges (23) of adjacent intermediate cell holders (9) touch, and wherein the end cell holder (10) has side edges (23) on the edges running perpendicular to the edge region (28) facing the temperature plate (16), which project beyond half of the adjacent individual cells (3), so that the side edges (23) of the end cell holder (10) and of the adjacent intermediate cell holder (9) touch.

The invention relates to an energy storage device (1) for storing electrical energy for a motor vehicle (14), comprising several stacked storage cells (3), preferably prismatic storage cells (3). An intermediate cell layer (2) is arranged between each pair of adjacent storage cells (3). The intermediate cell layer has a spacer (4) and a compression element (5), preferably a compression pad, wherein the spacer (4) has a higher thermal conductivity and a lower compressibility compared to the compression element (5). The compression element (5) is dimensioned and arranged relative to the spacer (4) such that at least one side (6) of the spacer (4), which faces one of the two adjacent storage cells (3), is completely covered by the compression element (5). The invention further relates to a motor vehicle (14) comprising such an energy storage device (1).

One embodiment of the semiconductor device comprises a first circuit cell and a second circuit cell, the latter adjoining the first circuit cell at an intermediate cell boundary. The first circuit cell has one or more first conductive traces in a first metallization region of a first metallization layer and has one or more first vias beneath the first metallization layer. The second circuit cell has one or more second conductive traces in a second metallization region of the first metallization layer and has one or more second vias beneath the first metallization layer. The first metallization region and the second metallization region are spaced apart by a common space and extend along the cell boundary.The one or more first via structures and the one or more second via structures are located within an area with a zigzag structure along the cell boundary.

The present invention discloses a portable energy storage battery structure, comprising an upper bracket, a lower bracket, and a cell mechanism embedded in the upper bracket and the lower bracket. The cell mechanism comprises side cell groups located on both sides and a plurality of intermediate cell groups located in the middle. The side cell groups on both sides are respectively connected to the total positive electrode or the total negative electrode of the cell mechanism via a first nickel sheet. The first nickel sheet has a bent double-layer structure, and the plurality of intermediate cell groups of the cell mechanism are respectively connected to a second nickel sheet. By designing the first nickel sheet as a bent double-layer structure, the nickel sheet converges evenly, so that the current of the total positive electrode or the total negative electrode converges toward the bend of the first nickel sheet. This bent double-layer process ensures that the cell will not be overloaded, and the temperature of the cell and the nickel sheet does not exceed the maximum temperature of the cell, thereby ensuring the reliability of the entire battery system.

The invention relates to a culture method of TIL from pleural effusion and / or ascites. Specifically, the invention provides the in-vitro culture method of the pleural effusion and / or ascites derived TIL, and the method comprises the following steps: 1) obtaining TIL seed cells from pleural effusion and / or ascites, and 2) sorting the TIL seed cells to obtain intermediate cells which are CD45RA-cells, CD39 + cells, or CD45RA-CD39 + cells.

Systems and methods for secure portals between virtual worlds are provided. In some embodiments, the systems and methods include deploying a secure portal between two virtual worlds. This is performed by rendering a top cell for a first virtual world. Rendering includes a transparent region overlapping a portal. Then an intermediate cell is layered underneath the top cell. This intermediate cell comprises the portal. A message is received from the top cell. The message includes a minimum rendering size, shape and at least one transform. This message is transferred to a bottom cell for a second virtual world, where it is rendered (in part) responsive to the message. The top cell, the intermediate cell and the bottom cell are then layered to generate a scene. In some embodiments, the rendered portion of the bottom cell comports to the minimum rendering size, and the intermediate cell is transparent. In some cases, the cells are either iFrames, or separate applications.

The invention provides a recombinant VSV pseudovirus as well as a preparation method and application thereof, and belongs to the field of pseudovirus synthesis. The method comprises the following steps: constructing packaging cells, helper plasmids and skeleton plasmids, separately transfecting the packaging cells with the helper plasmids and the skeleton plasmids, transfecting only one plasmid each time in the transfection process, transfecting the obtained cells with the next plasmid, and carrying out transfection for multiple times to obtain the recombinant VSV pseudovirus. And further performing pseudovirus amplification by continuously transfecting a cell strain expressing the outer membrane protein of the virus. The transfection process can be completed in the six-hole cell culture plate, the required reagent amount is small, and the cost is not obviously increased; the success rate is high when the delta G-VSV virus is packaged in the later period, the titer of the obtained pseudovirus is high, and the experiment repetition rate is obviously reduced; the intermediate cell strain obtained in the transfection process can be cryopreserved and can be directly used for packaging after being recovered, and the construction process does not need to be repeated.