24 results about "Leaf cell" patented technology

The invention relates to the field of molecular cytogenetics and potato ploidy breeding, in particular to a potato ploidy identification method for marking and counting chromosome centromere based on a potato CENH3 antibody and a ploidy detection kit. According to the method, the chromosome centromere is marked by specifically recognizing the antibody of the potato centromere nucleosome core histone CENH3 and combining the immunofluorescence technology, so that the accurate counting of the chromosome centromere is realized at the single cell level, and the ploidy of potatoes is judged. The method comprises the following steps: preparing a leaf cell suspension, sequentially incubating a potato CENH3 antibody and a labeled secondary antibody after immobilization, and observing and counting by a microscope after redyeing. The method has the advantages of high species specificity, high throughput and low cost, is not limited by special material taking parts of root tips or shoot tips and cell division periods, can accurately identify the uploid and aneuploid of potatoes, and provides an efficient tool for potato germplasm resource evaluation and cross breeding.

The invention provides a digital breeding-oriented Chinese cabbage leaf cell automatic segmentation and phenotype measurement system and method, and relates to the technical field of plant phenotype analysis. According to the system, joint coding of cell contours and tissue semantics is achieved through a feature extraction sub-network, scale alignment, global feature convergence and feature re-calibration, and pixel-level cell instance masks and tissue categories are output at the same time through mask decoding and category decoding. In combination with multi-tissue phenotypic parameter classification, in-tissue phenotypic index summarization and structured output, cell-level and tissue-level automatic quantitative analysis of upper epidermis, lower epidermis, fence tissues, sponge tissues, vascular bundles and the like is realized, and the efficiency and precision of multi-tissue cell screening and phenotypic statistics are remarkably improved. The method solves the problems that in the prior art, high-precision automatic segmentation and systematic phenotypic measurement for multi-tissue cells of the cross section of the Chinese cabbage leaf are insufficient, and digital breeding decision making is difficult to directly serve.

The present application relates to a kind of anti-aging composition and its application of brightening and shrinking pores.The composition includes the following components: halocyprid extract, purple magnolia extract, desert rose leaf cell extract, rain lily labiatae flower extract and white lily extract, and the mass ratio of the halocyprid extract, purple magnolia extract, desert rose leaf cell extract, rain lily labiatae flower extract and white lily extract is (0.2-2):(0.001-0.1):(0.001-0.1):(0.001-0.1):(0.001-0.1).The composition of the present application can effectively improve the clearance rate of collagenase, maintain the elasticity and tightness of skin, have good anti-aging and pore-shrinking effect, and can also effectively remove hydroxyl radicals, have good whitening and brightening effect.

The invention discloses a valve preparation method. The method comprises the following steps: performing decellularization treatment on a bovine pericardium material to be treated; enhancing the porosity of the decellularized bovine pericardium material through a physical and / or chemical method so as to promote the regeneration of native valve leaflet cells and tissues, and obtaining a composite material; and cutting the leaflet of the composite material to obtain the valve. The bovine pericardial valve which is high in durability, free of anticoagulation, excellent in biocompatibility, excellent in calcification resistance and low in immunogenicity can be prepared, and therefore the purpose of treating heart valve diseases is achieved.

The invention relates to an anti-aging, brightening and pore-shrinking composition and application thereof. The composition comprises the following components: an artemia extract, a magnolia liliiflora extract, a desert rose leaf cell extract, a lilium lanceolarium extract and a lilium fortunei extract. The mass ratio of the artemia extract to the magnolia liliiflora extract to the rosa deserticola leaf cell extract to the lilachium lanceolatum extract to the lilium fortunei extract is (0.2 to 2) to (0.001 to 0.1) to (0.001 to 0.1) to (0.001 to 0.1) to (0.001 to 0.1) to (0.001 to 0.1). The composition provided by the invention can effectively improve the collagenase clearance rate, maintain the elasticity and compactness of the skin, achieve good anti-aging and pore-shrinking effects, also can effectively remove hydroxyl radicals, and has good whitening and brightening effects.

This invention discloses Chinese kale BoaFLZ35 The application of genes in promoting vegetative growth of plants. This invention utilizes a gene-based approach to construct a *Gynostemma pentaphyllum* plant. BoaFLZ35 Heterologous overexpression of the gene in Arabidopsis thaliana was used to observe and analyze its effects on vegetative growth traits such as root elongation and rosette leaf morphology in transgenic plants. The results showed that heterologous overexpression... BoaFLZ35 This gene can significantly increase root cell length, thereby promoting the elongation of the Arabidopsis taproot; simultaneously, it can also increase leaf cell volume, resulting in significantly larger rosette leaves in transgenic plants. Therefore, it is evident that... BoaFLZ35 This is an important gene in the FLZ family of plants that positively regulates vegetative growth. This invention provides new insights into the function of FLZ family genes in the genus Brassica and also provides important candidate genes for high-yield molecular breeding of Brassica vegetables such as Chinese kale.

The invention discloses an exocytosis protein subcellular localization method for mannitol-induced plasm-wall separation, and relates to the technical field of cytobiology, the method comprises the following specific steps: constructing a fusion expression vector: connecting a plant target exocytosis protein gene with a fluorescent protein gene through a gene cloning technology to obtain a fusion expression vector; inserting into a plant expression vector to obtain a recombinant expression vector containing the target extracellular secretory protein-fluorescent protein fusion gene; performing bacterial colony PCR identification and sequencing verification on the recombinant expression vector; by adopting an agrobacterium tumefaciens-mediated plant leaf instantaneous transformation technology, the fusion gene is rapidly expressed in plant cells, subsequent experiments can be carried out after culture is carried out for 2-3 days, the experiment period is greatly shortened, meanwhile, leaf cells are induced to be subjected to plasmon-wall separation through a gradient mannitol solution, positive cell screening and identification are not needed, and the application prospect is wide. And positioning can be realized by directly observing fluorescence signal distribution by using a laser confocal microscope.

A fluid sensor device for measuring properties of a fluid is disclosed. The fluid sensor device includes a leaf cell sensor having a piezoelectric structure acting on a subdomain of the fluid that flows through the piezoelectric structure to create an intrinsic Helmholtz cavity response, and an enclosure enclosing the leaf cell sensor and including (i) a flowthrough shroud having an inlet that allows the fluid to enter the enclosure and pass across the leaf cell sensor, and a Helmholtz cavity wall that couples the intrinsic Helmholtz cavity response with an external acoustic field of the leaf sensor to increase a measurement sensitivity, (ii) a cylindrical housing having an outlet that allows the fluid to exit the enclosure, and (iii) a pressure feedthrough connector that transmits an electrical signal induced by the intrinsic Helmholtz cavity response to represent the properties of the fluid.

The invention discloses a method suitable for evaluating the top-free flooding resistance of a tropical water lily stock plant and a fetal seedling, and relates to the field of adversity stress, and the water lily stock plant or the fetal seedling is subjected to top-free flooding treatment with different time lengths. Determining the damage condition by taking the leaf length, the leaf width, the leaf ear length, the leaf area, the stem length, the stem diameter, the chlorophyll content and the leaf cell structure of the normally growing and waterlogged plant as evaluation indexes, and calculating the leaf rot index and the flood tolerance index of the to-be-detected water lily according to the flood damage leaf rot area to realize evaluation. According to the method, the influence of waterlogging on the external phenotype and internal structure of the water lily is considered, the difference of waterlogging treatment on plant ages is considered, the waterlogging resistance is determined through phenotype and cell structure changes, the waterlogging resistance level of the tropical fetal water lily is determined, and the method has the remarkable advantages of being short in evaluation period, visual, reliable and the like. The method is beneficial for promoting innovation of water lily flooding-resistant germplasm and breeding of a flooding-resistant fetal water lily variety, and a new method is provided for research of a water lily flooding-resistant mechanism.

The application provides a crop water requirement dynamic calculation method and an intelligent irrigation system, and relates to the technical field of agricultural irrigation.The crop water requirement dynamic calculation method adopts a quantum dot fluorescence probe array to accurately obtain soil water activity with a 0.1mm spatial resolution, and the accuracy is improved by 3-5 times compared with that of a traditional sensor; terahertz spectrum technology is used to analyze leaf cell sap osmotic pressure, so that nondestructive detection of crop physiological water requirement signals is realized, destructive sampling is avoided, a multi-modal fusion model is used in combination with transfer learning and a quantum correction factor, the water requirement calculation error is less than or equal to 3%, the dynamic response speed is improved to the hour level, the blockchain technology ensures data authenticity, the flow control accuracy of a magneto-rheological actuator reaches + / - 2% FS, and the water saving rate is more than 30%.

The present application relates to the field of plant molecular biology and genetic engineering technology, and particularly relates to a transient expression method of orchid plants and application. The method provided by the present application successfully breaks through the infection barrier of the thick cuticle of orchid plants by optimizing the core parameters of vacuum-assisted Agrobacterium infection, screening the infection strains and optimizing the composition of the infection solution. The high activity of the leaf cells can be maintained for more than 9 days after infection, and the exogenous genes can realize stable and efficient expression. The method provided by the present application is simple to operate, does not require special instruments and equipment, is convenient to obtain materials, is not limited by the flowering season, can be carried out throughout the year, and the complete experimental cycle can be completed within 7 days. The present application effectively solves the long-standing technical problems in the field of genetic transformation of orchid plants, and provides an efficient and convenient gene function transient verification technology platform for the functional genomics research, gene function verification, promoter activity detection and precise molecular breeding of orchid and other orchid plants.

The application describes a large scale process for the in vitro production of an olive cell culture. The application further describes a composition in a form of a powder comprising olive fruit / leaf cells grown in vitro. The cell culture of olive cells manufactured in vitro in a large scale according to the process of the invention comprises high level of verbascoside with undetectable levels of tyrosol, hydroxytyrosol, and oleuropein.

The application discloses a method for increasing drought adaptability of plant osmotic regulation substance accumulation, and belongs to the field of plant genetic engineering and drought-resistant molecular breeding. First, a plant to be transformed is combined with 13C-glutamic acid and 15N-choline double isotope labels to quantize carbon and nitrogen flow redistribution of proline synthesis pathways and glycine betaine synthesis pathways in leaf cells and vascular bundle companion cells, and to identify rate-limiting enzyme nodes. Second, a multi-cistronic plant expression vector is constructed. A first expression box is driven by a leaf-specific promoter and a drought response cis-element composite promoter to drive P5CS. A second expression box is driven by a companion cell-specific promoter and a drought response cis-element composite promoter to drive BADH. Positive transformants are obtained through Agrobacterium mediation or gene gun transformation and molecular screening. The expression quantity of the positive transformants is increased by 5 to 50 times under stress and falls back to a baseline within 72 hours of rehydration. Root activity is significantly improved, and yield is preserved.

The invention relates to the technical field of plant tissue culture, in particular to a medium-grain coffee embryogenic callus induction method, which comprises the following steps of: firstly, treating substances such as 5-azacytidine in darkness in an epigenetic reprogramming period; then, in a dedifferentiation stage, inducing callus through hormones such as 2, 4-D and the like; then, in the embryogenic induction period, the ratio of hormone to antioxidant is adjusted, and embryogenic differentiation is promoted; and finally, in an embryogenic maintenance period, adding a plurality of antioxidant and active substances, and optimizing culture conditions. According to the method, through multi-stage and multi-component fine regulation and control, the high-quality embryonic callus with the induction rate as high as 98%-99% can be obtained, the epigenetic regulation and control network effectively breaks through the epigenetic obstacle of differentiated leaf cells, and the induction rate is increased to 98%-99% from 30%-50% in the prior art.

The treatment method of the ginseng stem and leaf cell exosome-like vesicles provided by the embodiment of the invention comprises the following steps: step 1, crushing ginseng stems and leaves to prepare homogenate; step 2, sequentially carrying out multi-stage purification on the homogenate to obtain a vesicle layer; 3, based on the vesicle layer, stabilizing treatment is carried out, freeze-dried powder of the ginseng stem and leaf cell exosome-like vesicles is obtained, the freeze-dried powder of the ginseng stem and leaf cell exosome-like vesicles is obtained through stabilizing treatment in the step 3, and the technical effect that a high-stability product is obtained after the ginseng stem and leaf cell exosome-like vesicles are purified is achieved. Technical support is provided for batch production of high-quality ginseng stem and leaf extracellular vesicle products, and the method can be applied to industrial production.

The present application belongs to the technical field of skin care anti-glycation, and discloses a night-overtime skin anti-glycation compound containing nymphaea alba extract, referred to as "overnight ease"; the core active components include nymphaea alba embryo extract liquid, cordyceps militaris and ganoderma lucidum compound fermentation filtrate, nymphaea alba leaf cell culture liquid, nicotinamide, carnosine and ergothioneine; each active component is compounded according to a preset ratio, the anti-glycation effect can be achieved through multi-pathway synergistic action, the plant mucopolysaccharide in the fermentation filtrate can construct a three-dimensional moisturizing network on the skin surface layer, form a continuous hydration microenvironment, and indirectly slow down the glycation reaction rate, so as to adapt to the anti-glycation skin care needs of night-overtime damaged skin. The anti-glycation efficiency of the compound is significantly better than that of conventional single-pathway anti-glycation formula and ordinary plant crude extraction formula, the compound can simultaneously improve the problems of skin color dark yellow, skin relaxation and abnormal sebum secretion induced by night overtime, is mild and non-sensitizing, is suitable for different skin types, and can be widely used in various skin care products.

The invention discloses application of a gene Lp1-SST in improving the drought tolerance of ryegrass, and under the condition of drought stress, the plant height of transgenic Lp1-SST ryegrass is remarkably improved, the tiller number is remarkably increased, and the leaf width is also remarkably improved compared with that of a control group. Under a drought condition, the relative water content of the transgenic ryegrass leaves is obviously higher than that of a control group, which shows that the water loss of the transgenic plant leaves is less. Under a drought condition, the leaf conductivity significance of the transgenic ryegrass is lower than that of a control group, which indicates that the leaf cell membrane ion permeability of the transgenic plant is low, and the damage degree of the cell membrane is lower than that of the control group. Under the drought condition, the chlorophyll content of leaves of the transgenic ryegrass is obviously higher than that of a control group, which indicates that chlorophyll is degraded under the drought stress condition, and the chlorophyll decomposition rate of the transgenic plant can be reduced. In conclusion, the drought tolerance of the transgenic ryegrass is higher than that of a control group, and the drought tolerance of the ryegrass can be improved by transferring the Lp1-SST.

The application belongs to the technical field of genetic engineering, and particularly relates to application of a TaHsfC3-4 gene in salt tolerance improvement of wheat. The gene TaHsfC3-4 has a registration number of OQ680124.1, and plays a significant role in regulating salt tolerance of wheat. By using a CRISPR / Cas9 gene editing technology, the wheat gene TaHsfC3-4 is knocked out to obtain a homozygous mutant. After salt stress treatment, the phenotype and physiological indexes of the TaHsfC3-4 gene knockout mutant are better than those of a wild type Fielder. Specifically, after salt stress treatment, the mutant has less dry leaves, good growth, and lower relative conductivity and malondialdehyde content of leaves, indicating that the leaf cell membrane antioxidant capacity of the wheat TaHsfC3-4 gene mutant is improved, the cell membrane integrity is better, and the salt tolerance of the mutant is enhanced. The application provides a new method for rapidly creating a salt-tolerant new wheat variety.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a TaHsfC3-4 gene in wheat salt tolerance improvement. The registration number of the gene TaHsfC3-4 is OQ680124.1, the gene TaHsfC3-4 has a remarkable effect in regulating and controlling the salt tolerance of wheat, the wheat gene TaHsfC3-4 is knocked out through a CRISPR / Cas9 gene editing technology, a homozygous mutant of the wheat gene TaHsfC3-4 is obtained, after salt stress treatment, the phenotype and the physiological index of the TaHsfC3-4 gene knockout mutant are superior to those of a wild type Fielder, and the gene TaHsfC3-4 gene knockout mutant has the advantages that the gene TaHsfC3-4 gene knockout mutant can be used for regulating and controlling the salt tolerance of wheat. The wheat TaHsfC3-4 gene mutant has the specific expression that dry leaves of the mutant after salt stress treatment are few, the growth vigor is good, and the relative conductivity and malondialdehyde content of the leaves are lower, so that the oxidation resistance of the leaf cell membrane of the wheat TaHsfC3-4 gene mutant is improved, the integrity of the cell membrane is better, and the salt tolerance of the mutant is enhanced. The invention provides a new method for rapidly creating a new salt-tolerant wheat variety.

The invention discloses an application of a gene CcLBD36 in regulating and controlling the size of pepper leaves. The nucleotide sequence of the gene CcLBD36 is shown as SEQ ID No.2. The invention also discloses an application of the gene CcLBD36 in regulating and controlling the size of pepper leaves. The gene provided by the invention realizes accurate regulation and control of leaf length, width and leaf area by regulating and controlling pepper leaf cell proliferation and morphogenesis, and solves the bottleneck problem of the existing pepper leaf character improvement technology.

The application discloses a moisturizing and antioxidant composition containing a malpighia emarginata tree extract and PDNR and belongs to the technical field of cosmetics. The composition comprises the following components: a malpighia emarginata tree bark extract, a chrysophyllum coccineum fruit extract, a adenium obesum leaf cell extract, a cornus officinalis fruit extract and polydeoxyribonucleotide (PDRN), and the composition has good moisturizing and antioxidant effects through the synergistic effect among the five components.

The application discloses a flexible wearable sensor for detecting net photosynthetic rate of plants and a preparation method and application thereof, and the sensor is composed of a fixing layer, a substrate layer, an electrode layer and a conductive layer, and stretchable net structures are formed at both ends of the substrate layer; the electrode layer is a concentric circle sensing electrode, so that a uniform radial alternating current field is generated, and the conductive layer is closely attached to the surface of the leaf to reduce the interface contact impedance. The detection system constructed by the sensor can effectively solve the technical problem that the net photosynthetic rate of plants cannot be monitored in a long-term, non-destructive, real-time and in-situ manner in the prior art according to the high correlation between the alternating impedance parameters of the plant leaves under a specific characteristic frequency and the ion exchange of the leaf cells, by in-situ collection of the impedance change of the leaves, and by combination of environmental parameters, to calculate the net photosynthetic rate.

1. The name of the design product: packaging box (myrrh leaf cell soothing essence). 2. The use of the design product: the design is used for the box of myrrh leaf cell soothing essence. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view. 5. The design claimed contains color.

The application discloses genes LpCbDR3 In the application of improving the salt tolerance and forage quality of rye grass, transgenic RNAi- LpCbDR3 The crude protein content and fat of the rye grass are significantly improved, and the ADF, NDF and Ash contents are significantly reduced. LpCbDR3 The transgenic rye grass has a significantly improved chlorophyll content compared with the control group, which indicates that chlorophyll is degraded under the salt stress condition, and the transgenic plant can reduce the chlorophyll decomposition rate. LpCbDR3 The relative conductivity (EL) is significantly reduced, which indicates that the transgenic plant has a low ion permeability of leaf cell membrane and a low damage degree of cell membrane compared with the control group, and the harm of salt stress to the plant is alleviated. LpCbDR3 The forage quality and salt tolerance of perennial rye grass can be improved.