76 results about "Leaf cell" patented technology

The invention relates to a preparation method of moss scanning electron microscope observation material. The method includes: sorting, to be more specific, sorting moss materials from biological crusts, and stripping leaves after cleaning; fixing, to be more specific, fixing the leaves in glutaraldehyde solution, and washing with buffer solution; dehydrating step by step with alcohol with gradient concentration; replacing, to be more specific, placing the leaves in ethanol-isoamyl acetate mixture for replacing, and placing the leaves in isoamyl acetate solution for replacing; critical point drying; loading onto a table. The method has the advantages that the method is simple and practical, and good in effect, the glutaraldehyde solution serving as the fixing solution is good in fixing effect on polysaccharide, glycoprotein, microtubule and the like, tissue cells do not become brittle after fixing, the influence of leaf cell surface tension can be avoided by the critical point drying, and the microstructures of samples are well preserved; reference is provided for the preparation of ideal moss samples, and the technical blank of related fields in China is filled.

The present invention relates to a transgenic plant which is tolerant to a salt, comprising one or more plant cells transformed with exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant. Also encompassed by the present invention are transgenic progeny and seeds of the transgenic plants described herein. Progeny transgenic plant grown from seed are also described. The present invention also relates to a construct comprising an AVP1 gene operably linked to a chimeric promoter designed to overexpress AVP1 or designed to down regulate endogenous pyrophosphatase. Plant cells (e.g., root cells, stem cell, leaf cells) comprising exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant cell are also the subject of the present invention. Also encompassed by the present invention are methods of making a transgenic plant described herein. Transgenic plants produced by the methods of making a transgenic plant as described herein are also a subject of the present invention. The present invention also relates to a method of bioremediating soil, a method of increasing the yield of a plant, a method of making a plant which is larger than its corresponding wild type plant, and a method of producing a transgenic plant which grows in salt water comprising introducing into one or more cells of a plant nucleic acid which alters expression of vacuolar pyrophosphatase in the plant. The transgenic plants of the present invention can also be used to produce double transgenic plants which are tolerant to a salt.

The invention discloses a preparation method and an application of a jasmine plant leaf cell extract. The preparation method comprises the following steps: (1) selecting jasmine plant leaves as raw materials; (2) weighing ethanol 10-15 times as much as the weight of the jasmine plant leaves, mixing the ethanol with the jasmine plant leaves to prepare feed liquid, and putting the feed liquid in an environment with a vacuum degree of 0.4-0.6 MPa, reacting for 1-15 minutes at a temperature of 50-60 DEG C and ultrasonic power of 420W; (3) filtering and collecting to obtain the jasmine plant leaf cell extract. In the invention, the jasmine plant leaf cell extract is extracted by a vacuum coupling ultrasonic extraction method, the extraction time is shortened, the product yield is high, the working efficiency is high and the product is safe; the jasmine plant leaf cell extract is added in such cosmetics as facial cream to achieving anti-aging and anti-ultraviolet beautifying effects.

The invention discloses a method for inducing flavonoids and terpene lactones to accumulate in plant leaves by using methyl jasmonate and application, and belongs to the technical field of biology. The method comprises the following step: when plant leaf cells are completely mature, spraying a 20-80mg/L methyl jasmonate solution onto the plant leaves for 1-2 times, so that a ginkgo biloba extractwith high active ingredient content can be obtained from the treated plant leaves through a conventional method. Compared with the prior art, the method has the advantages of being simple and easy toimplement, low in cost, free of pollution, stable and efficient; the flavonoids and terpene lactones obtained after regulation and control by the method disclosed by the invention are high in content;wherein the contents of quercetin, kaempferol, isorhamnetin, total flavonoids, ginkgo terpene lactone A, ginkgo terpene lactone B and total terpene lactone are respectively increased by 29.84%, 18.08%, 35.94%, 26.24%, 30.36%, 60.65% and 35.41% compared with a control group.

The invention discloses a separation and purification method for Vaccinium bracteatum Thunb. leaf polysaccharide and blueberry leaf polysaccharide. The method includes the steps that fresh Vaccinium bracteatum Thunb. leaves and blueberry leaves are adopted as raw materials, cell disruption is carried out through the ultrasonic technology, extraction of polysaccharide is carried out with an alkaline solution, crude polysaccharide purification is carried out with composite biological enzymes, and pure natural plant polysaccharide ingredients are obtained. The method includes the four steps of Vaccinium bracteatum Thunb. leaf and blueberry leaf cell wall disruption, primary separation of Vaccinium bracteatum Thunb. leaf polysaccharide and blueberry leaf polysaccharide, purification of Vaccinium bracteatum Thunb. leaf polysaccharide and purification of blueberry leaf polysaccharide. The Vaccinium bracteatum Thunb. leaf polysaccharide and blueberry leaf polysaccharide products obtained after separation and purification with the method mainly have the effects of growth and development promotion, virus resistance, immune function enhancement, regulation and control of the intestinal microecological environment and promotion of development of immune organs on the human body.

A method of designing a logic circuit includes providing a leaf cell having at least one transistor. The leaf is suitable for use as a 1-cell or a 0-cell in the logic circuit. A first array of abutting leaf cells is tiled using at least one 1-cell and at least one 0-cell to define at least one logical expression by the relative positions of the array cells. Length optimized interconnects are added to the array. Each length optimized interconnect terminates at a last leaf cell in the array to which the interconnect makes contact. The leaf cell may be a floating leaf cell in which any pair of abutting cells are electrically isolated from one another until the length optimized interconnects are added to the design. The leaf cell array likely includes a set of rows and a set of columns in which the leaf cells in each row and the set of columns each correspond to an input of the logical expression.

The invention discloses a method for making high-fermentation elder leaf black tea. The method comprises the following steps: taking elder leaves of Wuyi rock tea trees as raw materials, picking fresh leaves, performing secondary wilting, performing alternative operations on three-time rolling and three-time fermentation, drying and the like. According to the invention, the unique rolling and wilting process is adopted, so that the elder leaf cells are uniformly and fully destroyed, and due to long-term fermentation, lots of substances contained in the elder leaves are fully oxidized and transformed, chemical changes of principle polyphenols and subsidiary transformation and accumulation of substances such as free amino acids, theanine, polysaccharides and the like are formed, the sufficiency and uniformity of tea leaf fermentation are effectively guaranteed, and the color, aroma, taste and shape of the tea leaves are improved, so that the made elder leaf black tea is tight and uniform in strip, oily in color, orange red and bright in soup, similar to dry aroma of longan, mellow in taste and obvious in sweetness after drinking tea.

The invention provides a method for extracting genome DNA from a dry suriana maritime leaf and belongs to the biotechnology field. Specifically, the extraction method provided by the invention comprises the following steps: grinding and crushing dry leaf cells, extracting DNA by adopting a two-step process, removing impurities and purifying the DNA. By adopting the extraction method provided by the invention, secondary metabolism products, such as polyphenol and polysaccharide, rich in a dry tissue sample can be effectively removed, and finally the high-quality genome DNA is obtained. The DNA extraction method provided by the invention has the advantages that polyvinylpyrrolidone is added, polyphenol substances can be more effectively removed, and quality of the genome DNA is obviously improved. The DNA extraction method provided by the invention is applicable to extraction of genome DNA from dry leaves of trees such as suriana maritime.

The present invention relates to a transgenic plant which is tolerant to a salt, comprising one or more plant cells transformed with exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant. The present invention also relates to a transgenic plant with increased Pi uptake, comprising one or more plant cells transformed with exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant. Also encompassed by the present invention are transgenic progeny and seeds of the transgenic plants described herein. Progeny transgenic plant grown from seed are also described. Plant cells (e.g., root cells, stem cell, leaf cells, flower cells, fruit cells and seed cells) comprising exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant cell are also the subject of the present invention. Also encompassed by the present invention are methods of making a transgenic plant described herein. Transgenic plants produced by the methods of making a transgenic plant as described herein are also a subject of the present invention. The present invention also relates to a method of bioremediating soil, a method of increasing the yield of a plant, a method of making a plant which is larger than its corresponding wild type plant, a method of producing a transgenic plant which grows in salt water, and a method of producing a transgenic plant with increased Pi uptake. The transgenic plants of the present invention can also be used to produce double transgenic plants which are tolerant to a salt, or have increased Pi uptake.

The invention belongs to the technical field of agricultural production, and particularly relates to a cold-resistant agent for brassica rapa L. The cold-resistant agent for the brassica rapa L. seedlings is mainly characterized by comprising raw materials as follows: 15-25 mg/L of ABA (abscisic acid) and the balance of water, wherein the purity of the ABA (abscisic acid) is higher than or equal to 99.9%. The cold-resistant agent aims to solve the problem that the brassica rapa L. in the northern cold and arid regions is vulnerable to low-temperature freezing damage, growth of the overground part and the root system of the brassica rapa L. is promoted with chemical control means, especially the thickening growth of roots, the outstanding performance refers to increase of dry matter accumulation, and safe wintering of the brassica rapa L. is guaranteed; meanwhile, permeability (relative electrical conductivity) of leaf cell membranes as well as accumulation of membrane lipid peroxidation malonaldehyde can be reduced, so that the low-temperature freezing degree of leaves of the brassica rapa L. is reduced, the cold resistance of the brassica rapa L. is remarkably improved, and the cold-resistant agent has great practical significance on brassica rapa L. production.

The invention belongs to the field of nano material biological effect research, and discloses a magnesium-nitrogen-doped carbon dot, a preparation method thereof and application of the magnesium-nitrogen-doped carbon dot in improvement of plant photosynthesis. The preparation method comprises the following steps: dissolving citric acid, ethanolamine and magnesium hydroxide in ultrapure water, carrying out ultrasonic treatment, pouring the treated system into a reaction kettle, and conducting reacting at 200 DEG C for 6 hours; after the reaction is finished, conducting cooling to room temperature, filtering a product by using a filter head with the pore diameter of 0.22 [mu]m, and then conducting dialyzing for 12 hours; and conducting freeze-drying to obtain the magnesium-nitrogen doped carbon dots. After the carbon dots are compounded with chloroplast, the excitation energy can be absorbed by the chloroplast for photosynthesis, so that the photosynthetic activity is improved. After the carbon dots are applied to rice plants through foliage spraying, Mg,N-CDs can be uniformly distributed in leaf cells, expression of related enzyme genes is regulated and controlled, synthesis and metabolism of chlorophyll in leaves are promoted, the activity of chlorophyll molecules is kept at a high level, and therefore the photosynthetic activity of the leaves is improved. Finally, the plant growth is obviously improved.

The invention relates to a method of promoting transplanted crops to absorb nutrients on the basis of high-voltage electrostatic fields. The method includes the following steps: 1 preparing seedling samples; 2 applying high voltage electric fields at different strengths to the samples; 3 measuring activity of seedling roots, penetration of leaf cell membranes and ionic concentration change of absorption liquid; 4 determining the optimum field intensity and treatment time which are favorable for nutrient absorption by the crops. The method of promoting transplanted crops to absorb nutrients on the basis of the high-voltage electrostatic fields can treat various different seedlings, determines the optimum field intensity and action time which can promote absorption of different nutrients for every kind of crop, and has the characteristics of being wide in application and simple to operate.

The invention discloses a method for determining a plant leaf cell conveying capacity, and belongs to the technical field of agricultural engineering and crop information detection. A determining device comprises a support, foam plates, electrode plates, lead wires, an iron block and a plastic rod; the foam plates with the inlaid electrode plates are adhered onto the bottom end of the support andthe plastic rod; when in use, the method comprises the steps of connecting the electrode plates and an LCR tester through the lead wires; clamping a plant leaf to be measured through the two electrodeplates, determining the physiological resistance, physiological impedance and physiological capacitance of the plant leaf through a parallel-connection mode, and calculating physiological capacitivereactance according to the physiological capacitance of the plant leaf; further calculating physiological inductive reactance of the plant leaf; adopting a physiological resistance reciprocal of the plant leaf as a reference, and obtaining a relative electric capacity and a relative carrying capacity of a plant leaf cell; further acquiring a conveying capacity of the plant leaf cell. The method provided by the invention can be used for quickly, losslessly and quantitively detecting the conveying capacities of different plant leaf cells on line, filling the blank of representing a cell conveying capacity by utilizing biological physical indexes, and providing a mode for the quantitation of a cell membrane function.

The invention discloses a preparation method of Dianhong Gongfu tea. The method comprises the following steps: a. withering, b. rolling, c. fermentation, d. drying. The premise of forming the excellent quality of black tea. On the one hand, rolling is to form cords, and on the other hand, it is to break leaf cells. It is an important process for shaping the shape and forming the inner quality of Gongfu black tea. Fermentation is a process in which the tea polyphenols in tea are oxidized violently under the action of enzymes, and at the same time affect the changes of other ingredients in the tea, forming the unique color and aroma of black tea. Fermentation is the key process to form the quality of Gongfu black tea. Drying is the last process to fix and improve the quality of black tea, which has a great influence on the quality. These four key steps directly affect the quality of tea. By refining the production process of Dianhong Gongfu tea, it can obtain a better taste and save costs.

The invention relates to a method for extracting tannin from wild persimmon leaves by an ultrasonic wave auxiliary semi-bionic method, which is mainly characterized in that the de-enzymed wild persimmon leaves are dried and smashed, ethanol and water as used as extraction solvents, and backflow extraction is carried out 3 times at the temperature of 50-60 DEG C by simulating the pH value of a gastrointestinal tract; and in the process of each extraction, ultrasonic waves are adopted for auxiliary treatment; and extracts in three times are combined and centrifuged, and then the obtained extract is concentrated and dried in a vacuum mode at the temperature of 50-60 DEG C to obtain the wild persimmon leaf tannin. In the semi-bionic extraction method of the invention, ultrasonic wave auxiliary extraction is adopted to make wild persimmon leaf cell tissues be wall-broken or deformed so as to increase the tannin yield, shorten the extraction time and keep the activity of the tannin.

The invention discloses a preparation method for peony leaf tea. A processing technology of a mechanical method and a biological enzymolysis processing technology are comprehensively utilized. The methods, such as, stirring and ultrasonically vibrating, are firstly utilized to break the cell walls of microorganisms so as to release the beneficial matters from the cells, and then the biological enzymolysis technology is utilized to extract the beneficial matters, such as, flavonoids, polyphenols, polysaccharides and alkaloids in the peony leaf cells. According to the preparation method, the peony leaves and the blades with healthcare values are perfectly combined with each other. The peony leaf tea has various benefits to the human body as follows: excellent effects of relieving the symptoms, such as, throat itching, dry throat and irritable cough caused by pharyngitis and effects of detoxifying, beautifying, nourishing, relieving pigmentation, relieving fatigue, resisting against ageing, diminishing inflammation, preventing cancers, preventing and treating cardiovascular and cerebrovascular diseases, and the like.

The present invention discloses novel application of ascorbic acid to improvement of plant photosynthesis efficiency. The invention is as below: using ascorbic acid solution with concentration gradient of 0.1,0.5,2,5,10 mmol / L to treat different varieties of plants for 24 hours, and respectively measuring physiological and biochemical parameters of leaves; stressing by using AlCl3 with different aluminum concentration gradients and different aluminum concentration gradients containing 2 mmol / L-ascorbic acid for 24 h, and measuring the photosynthetic characteristic parameters and physiological and biochemical indices of leaves respectively; and comparing the experimental results with the control plants. After ascorbic acid administration, H2O2 accumulation in aluminum sensitive soybean and Al tolerant soybean leaf cells decrease, and H + - pump activity and stomatal aperture and photosynthetic parameters are restored and improved; and these changes help promote plant stomatal opening and enhance photosynthesis and respiration of soybean, thereby enhancing the resistance to aluminum stress of plants.

The invention belongs to the technical field of cosmetics, and provides a whitening and moisturizing mask and a preparation method thereof. The whitening and moisturizing mask comprises the followingcomponents in parts by weight: 2-5 parts of arbutin, 0.7-1.5 parts of tranexamic acid, 0.8-1.5 parts of a desert rose leaf cell extract, 1-3 parts of nicotinamide, 0.5-2 parts of a glycyrrhiza glabraroot extract, 5-20 parts of a ceramide nanoemulsion, 1.7-3.5 parts of ascorbic acid, 1-5 parts of a rhizoma menispermi extract, 0.9-2.5 parts of a luffa root extract, 0.5-2 parts of a manyflower gueldenstaedtid herb extract, 0.1-0.3 part of polyphosphate double salt, 0.9-2.5 parts of polyacrylic acid, 0.1-0.5 part of butanediol, 0.9-2.5 parts of glycerinum and 25-43 parts of water, wherein the ceramide nanoemulsion consists of the following components in parts by weight: 1-5 parts of ceramide, 2-10 parts of olive oil, 5-15 parts of glycerol, 3-8 parts of castor oil polyoxyethylene ether, 1.5-4parts of poloxamer and 35-62 parts of water. By adopting the technical scheme, the problem that in a mask in the prior art, ceramide is poor in dissolution and stability is solved.

The present invention refers to the use of an extract of soluble proteins from plant leaf cells for the preparation of subunit vaccines comprising said extract as adjuvant, to the vaccines and to the kit comprising said extract as adjuvant and to the processes for the preparation thereof. the present invention further refers to a plant obtainable by the step of expressing in soluble from a protein antigen in leaf cells at a concentration greater than or equal to 3 mug of protein per gram of leaf.