1250 results about "Transgenesis" patented technology

The invention provides application of an ECT2 gene in regulating and controlling development of secondary xylem of a poplar. Transgenic poplar with overexpressed ECT2 genes is obtained through genetic transformation, it is found that overexpressed ECT2 genes can increase the width of secondary xylem of the poplar, and the nucleotide sequence of the ECT2 genes is shown as SEQ ID NO: 1. The ECT2 gene has a regulation effect on the development of the secondary xylem of the poplar.

The invention belongs to the field of molecular biology, and particularly relates to a transgenic soybean event LP207-1 and a detection method thereof. The invention provides a nucleic acid sequence for detecting a transgenic soybean event LP207-1, the nucleic acid sequence is selected from one or more of sequences SEQ ID NO: 1-7 or complementary sequences thereof, and the nucleic acid sequence is derived from a plant, a seed or a cell of the transgenic soybean event LP207-1. A representative sample of a seed of the transgenic soybean event LP207-1 is preserved in the China Center for Type Culture Collection on May 23, 2025, and the preservation number is CCTCC NO: P202516. The transgenic soybean event LP207-1 has good resistance to lepidoptera pests and can tolerate agricultural herbicides containing glyphosate and glufosinate-ammonium, economic loss caused by the lepidoptera pests can be avoided, and the yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking a breeding population and transgenic insertion fragments in offspring of the breeding population. The detection method provided by the invention can quickly, accurately and stably identify the existence of the plant material derived from the transgenic soybean event LP207-1.

The invention provides a method for preparing a chicken immortalized cell line. The method comprises the following steps: constructing a plasmid system of conditional over-expression TERT (Telomerase Reverse Transcriptase), carrying out ovarian injection and breeding to obtain a transgenic chicken of the conditional over-expression TERT, and carrying out separation and induction to obtain the chicken immortalized cell line. The obtained chicken immortalized cell line has the characteristics of primary cells and is susceptible to viruses; the method can be used for virus reproduction and detection, influenza virus vaccine production and the like, can obtain immortalized cell lines of all organs and all parts of the chicken in a short time, and has a good application prospect in the vaccine industry.

The invention provides application of a soybean GmWRKY40 gene in soybean breeding, and belongs to the technical field of functional gene breeding. The nucleotide sequence of the soybean GmWRKY40 gene provided by the invention is as shown in SEQ ID NO: 1. According to the invention, the GmWRKY40 gene is constructed into a skeleton expression vector to obtain a recombinant vector, and the recombinant vector is transferred into soybean under the mediation of agrobacterium tumefaciens to obtain a transgenic plant with stable overexpression of the GmWRKY40 gene. Detection results show that overexpression of the GmWRKY40 gene can significantly improve the grain isoflavone content and the protein oil content of the transgenic soybean, improve the soybean quality and increase the soybean grain yield at the same time. Therefore, the soybean GmWRKY40 gene can be used for developing new varieties of high-quality soybeans.

The invention discloses a gene PtoMYB20 for regulating and controlling salt tolerance and drought resistance of poplars. A nucleotide sequence of the gene comprises a sequence as shown in SEQ ID NO. 1. The gene interference fragment is introduced into a plant to obtain a silent transgenic plant, under the conditions of salt stress, drought stress and salt-drought composite stress, the H2O2 and MDA contents of the silent transgenic plant are lower than those of a wild plant, and the SOD and POD activities of the silent transgenic plant are higher than those of the wild plant, so that the damage degree of the plant is effectively reduced; the method is of great significance in improving salt tolerance and drought resistance of the poplar and relieving damage of adversity stress to forestry yield. The invention also discloses a breeding method of the transgenic poplar with strong salt tolerance and drought resistance, provides a new poplar germplasm which not only improves the stress resistance of the poplar but also does not influence the wood yield, and lays a theoretical and gene resource foundation for the stress-resistant molecular breeding of the poplar.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an exogenous gene integration site, a homologous recombinant vector and a construction method of a transgenic pig. According to the present invention, the safety site integrated by the exogenous gene provided by the present invention is the region between the LOC100155859 tubulin alpha-1B chain-like gene and the LOC100517052 histone H3.3-like gene of the pig chromosome 11, and is the region between the LOC100155859 tubulin alpha-1B chain-like gene and the LOC100517052 histone H3.3-like gene of the pig chromosome 11; the safe site is further fully verified on transgenic animals and cells, and the exogenous gene can be stably and efficiently expressed in the passage process and has no side effect on the cells and tissues. Therefore, the safe site provided by the invention has a good application prospect in gene engineering.

A transgenic soybean event, Gm_CSM63717, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gm_CSM63717 are also provided. Recombinant DNA molecules unique to the event Gm_CSM63717, and methods of using and detecting Gm_CSM63717 are also provided. Soybean plants containing the event Gm_CSM63717 exhibit tolerance to PPO inhibitors.

The invention provides an establishment method of a transgenic mouse, and the method comprises the following steps: a transgenic mouse MHC chimeric gene is introduced into an endogenous B2m gene locus of the mouse, the MHC chimeric gene comprises a human B2M gene and a chimeric alpha-chain gene, and the chimeric alpha-chain gene comprises a human alpha1 region, a human alpha2 region and a mouse alpha3 region; the chimeric alpha-chain gene further comprises a mouse transmembrane region and a mouse intracellular region. The transgenic mouse disclosed by the invention can be applied to tumor specific antibody detection, in-vivo evaluation of vaccines, and in-vivo screening of TCR-T and other drugs.

The invention discloses a grape seed development gene VvPHERES1 and an application of the grape seed development gene VvPHERES1. The nucleotide sequence of the developmental gene VvPHERES1 is as shown in SEQ ID NO: 1. The amino acid sequence of the TypeI MADS transcription factor is as shown in SEQ ID NO: 2. According to the invention, the grape seed development related gene VvPHERES1 is cloned for the first time, and the gene function of the grape seed development related gene VvPHERES1 is researched. The method comprises the following steps: constructing a pCAMBIA2300-VvPHERES1 overexpression vector, and carrying out heterologous transformation on a Micro-Tom tomato plant. By comparing the change of seed characters in the overexpressed transgenic tomato and the wild type plant, the result shows that the number of seeds in the overexpressed transgenic tomato is obviously increased. The invention provides a gene related to regulation and control of grape seed development, and a basis is provided for seed selection of new seedless grape varieties.

The invention belongs to the technical field of plant breeding and biology, and particularly relates to how to reduce the cadmium content of rice grains through a gene OsCT1 and application of the gene OsCT1 to creation of new germplasm of low-cadmium-accumulation rice. The invention discloses a nucleotide sequence of a gene OsCT1 coding region, a coded protein sequence, a CRISPR-Cas9 knockout target site nucleotide sequence, a nucleotide sequence of a knockout mutant and a protein sequence of the knockout mutant, as shown in an ST.26 standard sequence nucleotide or amino acid sequence table. After the gene OsCT1 is knocked out by utilizing a CRISPR-Cas9 technology, the cadmium content of grains of the OsCT1-knocked-out transgenic plant is obviously lower than that of a wild type variety. In addition, in the seedling stage, the plant height and the root length of the OsCT1-knocked-out transgenic plant are remarkably larger than those of a wild type variety; after the OsCT1 gene is subjected to overexpression, the grain cadmium content of an overexpressed transgenic plant is slightly higher than that of a wild type variety. In addition, in the seedling stage, the plant height and the root length of the overexpressed OsCT1 transgenic plant are obviously smaller than those of a wild type variety. According to the invention, the OsCT1 gene can be utilized to create a novel rice germplasm with low cadmium content in grains.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a KASP marker for detecting yellow 6106 in transgenic soybeans and application of the KASP marker. The specific sequence where the KASP marker is located comprises ZH6106-2R and / or ZH6106-3R, and the specific sequence where the KASP marker is located comprises ZH6106-2R; the nucleotide sequence of the ZH6106-2R is as shown in SEQ ID NO: 1; the nucleotide sequence of the ZH6106-3R is as shown in SEQ ID NO: 2. Through the KASP marker and the primer, whether a sample to be detected contains the yellow 6106 component in the transgenic soybean or not can be effectively detected, and the detection method is rapid, simple, convenient, accurate and reliable.

Disclosed herein is a mutant, non-naturally occurring or transgenic plant or plant part having reduced or inhibited expression or activity of NtNTP2-T, or reduced or inhibited expression or activity of NtNTP2-T and NtNTP2-S, the NtNTP2-T and NtNTP2-S comprising or consisting of: (i) a polynucleotide sequence of NtNTP2-S comprising at least one of a nucleotide sequence of NtNTP2-S and a nucleotide sequence of NtNTP2-S comprising at least one of a nucleotide sequence of NtNTP2-S, a nucleotide sequence of NtNTP2-S, a nucleotide sequence of NtNTP2-S, and a nucleotide sequence of NtNTP2-S comprising at least one of a nucleotide sequence of NtNTP2-S, and a nucleotide sequence of NtNTP2-S; a nucleotide sequence comprising, consisting or consisting essentially of a sequence having at least 70% sequence identity to SEQ ID NO: 3; or (ii) an NtNTP2-T polynucleotide sequence comprising, consisting or consisting essentially of a sequence having at least 70% sequence identity to SEQ ID NO: 11; or (iii) a polypeptide encoded by the polynucleotide shown in (i) or (ii); or (iv) an NtNTP2-S polypeptide having at least 77% sequence identity to SEQ ID NO: 7; or (v) an NtNTP2-T polypeptide having at least 77% sequence identity to SEQ ID NO: 12; wherein the expression or activity of NtNTP2-T or the expression or activity of NtNTP2-T and NtNTP2-S is reduced or inhibited as compared to a control plant.

The invention belongs to the technical field of biology, and particularly relates to application of a soybean GmSRO5 gene in promoting generation of leguminous plant root nodules. A pZP211 overexpression vector is utilized, a transgenic plant is rapidly obtained through agrobacterium tumefaciens-mediated soybean genetic transformation, GmSRO5 is successfully overexpressed, root nodule number statistics is carried out on a chimera transgenic plant, and a result shows that the GmSRO5 gene can positively regulate and control the number of soybean root nodules and has a remarkable effect. The invention proposes that SRO5 can positively regulate and control the soybean nodulation process for the first time, and proves that the soybean GmSRO5 gene overexpression can significantly increase the number of soybean root nodules, and the soybean GmSRO5 gene overexpression can be applied to soybean production and molecular breeding through transgenosis, molecular marking and other means in the future, and has very important application value.

The invention discloses a GmbZIP36 protein related to aluminum resistance of plants in acid soil as well as a coding gene and application of the GmbZIP36 protein, and belongs to the technical field of bioengineering. The amino acid sequence of the GmbZIP36 protein is shown as SEQ ID NO: 2, 3 or 4, and the nucleotide sequence of the coding gene of the GmbZIP36 protein is shown as SEQ ID NO: 1. The invention finds that the expression of the GmbZIP36 gene is induced by aluminum stress, the relative root elongation and root biomass of arabidopsis thaliana and soybean under the aluminum stress can be obviously improved by over-expressing the gene in a plant, and aluminum accumulation is reduced, so that the aluminum resistance of the plant is enhanced. The invention provides a recombinant vector containing the gene, a host cell and application of the recombinant vector and the host cell in regulating and controlling the aluminum resistance of plants. Meanwhile, the invention also provides a method for producing an aluminum-resistant transgenic plant by using the gene, a kit for detecting the gene or protein and a method for carrying out assisted breeding by using the molecular marker linked with the gene. The invention provides a key gene resource for aluminum-resistant molecular breeding of crops, and has important significance for cultivating new varieties of crops adapting to acid soil.

Provided is a nucleic acid sequence, comprising one or more selected from sequences SEQ ID NOs: 1-7 or complementary sequences thereof. The nucleic acid sequence is derived from a transgenic soybean event LP086-1, and the preservation number of a representative sample of seeds of the transgenic soybean event LP086-1 is CCTCCNO: P202325. The transgenic soybean event LP086-1 has the following advantages: avoiding the economic loss caused by lepidopteran pests; being resistant to a common commercial herbicide, i.e., glyphosate; not reducing the soybean yield; enhancing the breeding efficiency; and being capable of using molecular markers to track transgenic insertion fragments in breeding populations and offsprings thereof. In addition, the provided detection method can quickly, accurately and stably identify the presence of plant materials derived from the transgenic soybean event LP086-1.

Transgenic rodents and rodent cells that express components of the human immune system, and their uses in generating human T cell receptors (TCRs). A genetically modified rodent comprising a TCR alpha gene locus comprising unrearranged human TCR alpha variable gene segments and a human TCR alpha constant region, and a TCR beta gene locus comprising unrearranged human TCR beta variable gene segments and a human TCR beta constant region. Use of hTCR transgenic rodents to generate antigen-specific CD8+ T cell responses for the discovery of fully human TCR sequences with therapeutic potential.

The invention discloses a gene RBOHB and application of the gene RBOHB in regulating and controlling waterlogging stress to induce cucumber adventitious root formation, a novel gene RBOHB for inducing cucumber adventitious root formation is identified, the adventitious root formation capability of an edited mutant of the gene RBOHB is weakened, the extension of the adventitious root is obviously inhibited, and the adventitious root formation capability of an overexpressed mutant is enhanced. The gene RBOHB disclosed by the invention is used for regulating and controlling the waterlogging tolerance of cucumbers, and has important application value in screening and improving waterlogging-tolerant germplasm resources of the cucumbers and breeding waterlogging-tolerant varieties of the cucumbers. The cucumber is subjected to transgenic genetic transformation through a gene editing technology and a gene overexpression technology, the cucumber waterlogging tolerance regulation gene RBOHB and application of the cucumber waterlogging tolerance regulation gene RBOHB in waterlogging tolerance cucumber varieties are provided, and the cucumber waterlogging tolerance regulation gene RBOHB has important application value in the field of breeding of resistant cucumbers.

The present invention relates to transgenic ornamental fish, as well as methods of making such fish by m vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

The invention belongs to the technical field of gene engineering, and discloses a gene for regulating tillering, dry weight and yield of rice and application of the gene. The gene is an NIP1 gene and is located on the sixth chromosome of rice, the nucleotide sequence of the NIP1 gene is as shown in SEQ ID NO.1, and the amino acid sequence coded by the NIP1 gene is as shown in SEQ ID NO.2. The NIP1 gene is cloned, a transgenic vector is constructed, an NIP1 overexpression plant is obtained, and the NIP1 overexpression plant can regulate and control tillering, dry weight and yield of rice. Through determination of overexpression plant characters, compared with control group rice, overexpression shows tillering, dry weight and yield increase characters under low-nitrogen and high-nitrogen conditions, which indicates that the NIP1 gene plays an important regulation function in the aspects of tillering, dry weight, yield and nitrogen utilization of rice.

The invention provides a method for producing borneol, the method comprising culturing the transgenic microorganism in a culture medium, and the microorganism comprises or expresses borneol diphosphate synthetase and phosphatase.

The invention discloses a construction method and application of a mouse model for specifically tracing heart valve cells, and belongs to the technical field of animal model construction. The model construction method comprises the following steps: (1) constructing a Wid1-Cre hybrid mouse before inserting a Cre recombinase coding gene into a termination codon of a mouse Wid1 gene; and (2) hybridizing the Wid1-Cre hybrid mouse with the Rosa26-tdTomato homozygous report mouse, so as to obtain the double transgenic mouse which simultaneously carries the Wid1-Cre gene and the Rosa26-tdTomato gene. According to the invention, on the basis of the expression characteristic that Wif1 is only limited to a valve area in the heart, Wif1-Cre is constructed; according to the Rosa26-tdTomato mouse model, specific tracing of heart valve cells is achieved, and an important experimental tool is provided for mechanism research of heart valve diseases and development of therapeutic drugs.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The invention relates to the technical field of life science, in particular to a method for constructing an atrophic gastritis-based SPEM lesion model, which comprises the following steps: S1, constructing an Slc7a11 conditional knockout mouse strain: carrying out cage hybridization on an Slc7a11 flox / flox mouse and an ATP4b-Cre transgenic mouse, and knocking out a gastric wall cell specific Slc7a11 gene; s2, carrying out genotyping identification; the identification method based on the atrophic gastritis SPEM lesion model and the construction method based on the atrophic gastritis SPEM lesion model comprise the following steps: S1, pathological model verification: taking a mouse gastric mucosa tissue slice, quantitatively analyzing the proportion of an SPEM region by adopting an immunofluorescence double-standard technology, and confirming Slc7a11 knockout induced SPEM pathological characteristics in combination with the existence of SPEM in the gastric mucosa; and S2, long-term pathology evolution monitoring. Through gastric wall cell specific Slc7a11 gene knockout and multiple PCR identification technologies, the initial response SPEM state after gastric mucosa damage is successfully simulated, and the kit is used for researching atrophic gastritis pathological mechanisms and evaluating drug intervention.

The invention relates to cloning of a stress-resistant gene LpbHLH75 of Lilium pumilum DC (Lilium pumilum DC.) and application of the stress-resistant gene LpbHLH75 of Lilium pumilum DC. The lilium tenuifolium stress resistance key genes are mined through transcriptome data analysis, and the functions of the lilium tenuifolium stress resistance key genes are studied. Comprising the following steps: (1) cloning a target gene; (2) constructing a plant overexpression vector; and (3) detecting physiological indexes of the transgenic plant. According to the experiment, lilium tenuifolium is taken as a research object, and the LpbHLH75 gene is cloned by applying an RT-PCR technology; transforming tobacco by constructing an overexpression vector; the function of overexpression of the LpbHLH75 on tobacco salt and alkali and drought stress is identified through transgenic tobacco, the function of the lilium tenuifolium bHLH gene under adversity stress is disclosed, a new gene source is provided for lilium tenuifolium genetic engineering breeding, a reference is provided for later lilium tenuifolium stress-resistant germplasm resource research, and the lilium tenuifolium bHLH gene is developed. And a foundation is laid for exploring a gene capable of enhancing stress resistance in the lilium tenuifolium and a stress resistance mechanism of the gene.

The invention belongs to the field of antibody development, and discloses a screening method and application of a human-mouse protein high homologous target antibody based on a fully humanized antibody mouse. Aiming at the problem of weak antibody response caused by immune tolerance of human-mouse high homologous targets (protein homology is greater than or equal to 95%), the following scheme is provided: in embryonic stem cells (ES cells) of HUGO-Mabfully humanized antibody transgenic mice, a mouse target gene (such as ACVR2A) is knocked out through a Turbo Knockout technology, and homozygous knockout ES clones are screened; carrying out microinjection on the clones to the whitened B6 mouse blastocyst, and transplanting a pregnant mouse to obtain a Founder mouse; the Founder mouse is subjected to target antigen immunization for more than or equal to 4 times (the Freund's complete adjuvant is used for the first time), and the titer of the serum antibody is detected. According to the invention, 100% homozygous knockout chimeric efficiency is realized in the Founder stage, the mouse construction period is shortened from traditional 8-10 months to 3-4 months, and the diversity and affinity of the antibody are significantly improved (titer reaches 1: 729,000). The obtained antibody can be used for preparing medicines for treating tumors or autoimmune diseases.

The invention belongs to the technical field of biology, and relates to a barley germination period salt-tolerant gene HvHCT1, a protein and an application of the protein in salt damage stress resistance, and the protein comprises an amino acid sequence shown as SEQ ID NO: 2. According to the method, genetic loci related to the salt tolerance of the barley in the germination period are mined through a whole genome association analysis method, then transcriptome sequencing is used for revealing barley salt tolerance differential gene expression changes after salt damage stress, and in combination with association analysis and transcriptome sequencing data, salt tolerance candidate genes in the germination period of the barley are determined. The transgenic arabidopsis thaliana is used for verification; the research provides an excellent germplasm material and a new salt-tolerant gene for barley salt-tolerant breeding research, and also provides a theoretical basis for barley salt-tolerant molecular marker-assisted breeding in the future.

The invention discloses a herbicide-resistant protein GAT hybridoma cell strain, an antibody generated by the herbicide-resistant protein GAT hybridoma cell strain and an application of the herbicide-resistant protein GAT hybridoma cell strain. The herbicide-resistant protein GAT hybridoma cell strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.46333. The preparation method comprises the following steps: a) purifying prokaryotic expression to obtain GAT recombinant protein; b) immunizing animals: immunizing BALB / c mice by taking the GAT recombinant protein as an antigen; c) cell fusion: splenocytes of the immune BALB / c mice are collected and fused with SP2 / 0 cells; and d) cell strain establishment: carrying out subcloning through a limited dilution method, carrying out ELISA detection after 5-7 days of subcloning until a hybridoma cell strain capable of stably secreting a positive antibody is screened out, and carrying out amplification, re-culture and storage. The monoclonal antibody secreted by the hybridoma cell strain lays a foundation for detection of herbicide-resistant protein GAT in transgenic crops, so that development, seed production and seed conservation of transgenic gat crops are supported, and stable industrial promotion of the transgenic gat crops and sustainable application of related transformants are guaranteed.

The present disclosure relates to the expression of recombinant viral antigens using transgenic fungi. Compositions, production and administration of vaccines comprising those viral antigens are also disclosed. In some embodiments, these viral antigens can be used to formulate vaccines against Newcastle disease. These vaccines may be administered, for example, by intramuscular injection.

The invention relates to the field of biological medicine, in particular to an adeno-associated virus as well as a preparation method and application thereof. The capsid protein of the adeno-associated virus AAV-ACM205 provided by the invention comprises a wild type AAV2 capsid protein or a variant of the wild type AAV2 capsid protein, the wild type AAV2 capsid protein or the variant of the wild type AAV2 capsid protein comprises peptide fragments with amino acid sequences as shown in SEQ ID NO.6 and SEQ ID NO.7, and a random peptide fragment is inserted between the SEQ ID NO.6 and the SEQ ID NO.7. The adeno-associated virus vector AAV-ACM205 provided by the invention can mediate a target gene to be specifically and efficiently expressed in hair cells in cochlea of an adult mouse under the condition of semicircular canal injection; the adeno-associated virus vector AAV-ACM205 mediated target gene expression is more flexible and efficient than that of a traditional transgenic method, is convenient to apply and low in cost, and has a wide prospect in ear disease gene therapy.

The invention provides a salt-tolerant gene, namely a PrABC1 gene and application thereof, and also provides a vector containing the gene, an expression cassette, a recombinant cell, a transgenic plant and a method for producing the transgenic plant. Specifically, the gene has a nucleotide sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 2. The plant growth regulator can be used for improving the tolerance of plants to a salt stress environment, relieving the adverse effects of a high-salt environment on the germination rate and root length of the plants, reducing the water loss rate of the plants under the high-salt stress condition and reducing the reduction of the chlorophyll content.