1004 results about "Transgenesis" patented technology

The invention provides application of an ECT2 gene in regulating and controlling development of secondary xylem of a poplar. Transgenic poplar with overexpressed ECT2 genes is obtained through genetic transformation, it is found that overexpressed ECT2 genes can increase the width of secondary xylem of the poplar, and the nucleotide sequence of the ECT2 genes is shown as SEQ ID NO: 1. The ECT2 gene has a regulation effect on the development of the secondary xylem of the poplar.

The invention belongs to the field of molecular biology, and particularly relates to a transgenic soybean event LP207-1 and a detection method thereof. The invention provides a nucleic acid sequence for detecting a transgenic soybean event LP207-1, the nucleic acid sequence is selected from one or more of sequences SEQ ID NO: 1-7 or complementary sequences thereof, and the nucleic acid sequence is derived from a plant, a seed or a cell of the transgenic soybean event LP207-1. A representative sample of a seed of the transgenic soybean event LP207-1 is preserved in the China Center for Type Culture Collection on May 23, 2025, and the preservation number is CCTCC NO: P202516. The transgenic soybean event LP207-1 has good resistance to lepidoptera pests and can tolerate agricultural herbicides containing glyphosate and glufosinate-ammonium, economic loss caused by the lepidoptera pests can be avoided, and the yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking a breeding population and transgenic insertion fragments in offspring of the breeding population. The detection method provided by the invention can quickly, accurately and stably identify the existence of the plant material derived from the transgenic soybean event LP207-1.

The invention discloses a gene PtoMYB20 for regulating and controlling salt tolerance and drought resistance of poplars. A nucleotide sequence of the gene comprises a sequence as shown in SEQ ID NO. 1. The gene interference fragment is introduced into a plant to obtain a silent transgenic plant, under the conditions of salt stress, drought stress and salt-drought composite stress, the H2O2 and MDA contents of the silent transgenic plant are lower than those of a wild plant, and the SOD and POD activities of the silent transgenic plant are higher than those of the wild plant, so that the damage degree of the plant is effectively reduced; the method is of great significance in improving salt tolerance and drought resistance of the poplar and relieving damage of adversity stress to forestry yield. The invention also discloses a breeding method of the transgenic poplar with strong salt tolerance and drought resistance, provides a new poplar germplasm which not only improves the stress resistance of the poplar but also does not influence the wood yield, and lays a theoretical and gene resource foundation for the stress-resistant molecular breeding of the poplar.

A transgenic soybean event, Gm_CSM63717, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gm_CSM63717 are also provided. Recombinant DNA molecules unique to the event Gm_CSM63717, and methods of using and detecting Gm_CSM63717 are also provided. Soybean plants containing the event Gm_CSM63717 exhibit tolerance to PPO inhibitors.

The invention provides an establishment method of a transgenic mouse, and the method comprises the following steps: a transgenic mouse MHC chimeric gene is introduced into an endogenous B2m gene locus of the mouse, the MHC chimeric gene comprises a human B2M gene and a chimeric alpha-chain gene, and the chimeric alpha-chain gene comprises a human alpha1 region, a human alpha2 region and a mouse alpha3 region; the chimeric alpha-chain gene further comprises a mouse transmembrane region and a mouse intracellular region. The transgenic mouse disclosed by the invention can be applied to tumor specific antibody detection, in-vivo evaluation of vaccines, and in-vivo screening of TCR-T and other drugs.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a KASP marker for detecting yellow 6106 in transgenic soybeans and application of the KASP marker. The specific sequence where the KASP marker is located comprises ZH6106-2R and / or ZH6106-3R, and the specific sequence where the KASP marker is located comprises ZH6106-2R; the nucleotide sequence of the ZH6106-2R is as shown in SEQ ID NO: 1; the nucleotide sequence of the ZH6106-3R is as shown in SEQ ID NO: 2. Through the KASP marker and the primer, whether a sample to be detected contains the yellow 6106 component in the transgenic soybean or not can be effectively detected, and the detection method is rapid, simple, convenient, accurate and reliable.

The invention belongs to the technical field of biology, and particularly relates to application of a soybean GmSRO5 gene in promoting generation of leguminous plant root nodules. A pZP211 overexpression vector is utilized, a transgenic plant is rapidly obtained through agrobacterium tumefaciens-mediated soybean genetic transformation, GmSRO5 is successfully overexpressed, root nodule number statistics is carried out on a chimera transgenic plant, and a result shows that the GmSRO5 gene can positively regulate and control the number of soybean root nodules and has a remarkable effect. The invention proposes that SRO5 can positively regulate and control the soybean nodulation process for the first time, and proves that the soybean GmSRO5 gene overexpression can significantly increase the number of soybean root nodules, and the soybean GmSRO5 gene overexpression can be applied to soybean production and molecular breeding through transgenosis, molecular marking and other means in the future, and has very important application value.

The invention discloses a GmbZIP36 protein related to aluminum resistance of plants in acid soil as well as a coding gene and application of the GmbZIP36 protein, and belongs to the technical field of bioengineering. The amino acid sequence of the GmbZIP36 protein is shown as SEQ ID NO: 2, 3 or 4, and the nucleotide sequence of the coding gene of the GmbZIP36 protein is shown as SEQ ID NO: 1. The invention finds that the expression of the GmbZIP36 gene is induced by aluminum stress, the relative root elongation and root biomass of arabidopsis thaliana and soybean under the aluminum stress can be obviously improved by over-expressing the gene in a plant, and aluminum accumulation is reduced, so that the aluminum resistance of the plant is enhanced. The invention provides a recombinant vector containing the gene, a host cell and application of the recombinant vector and the host cell in regulating and controlling the aluminum resistance of plants. Meanwhile, the invention also provides a method for producing an aluminum-resistant transgenic plant by using the gene, a kit for detecting the gene or protein and a method for carrying out assisted breeding by using the molecular marker linked with the gene. The invention provides a key gene resource for aluminum-resistant molecular breeding of crops, and has important significance for cultivating new varieties of crops adapting to acid soil.

The invention belongs to the technical field of gene engineering, and discloses a gene for regulating tillering, dry weight and yield of rice and application of the gene. The gene is an NIP1 gene and is located on the sixth chromosome of rice, the nucleotide sequence of the NIP1 gene is as shown in SEQ ID NO.1, and the amino acid sequence coded by the NIP1 gene is as shown in SEQ ID NO.2. The NIP1 gene is cloned, a transgenic vector is constructed, an NIP1 overexpression plant is obtained, and the NIP1 overexpression plant can regulate and control tillering, dry weight and yield of rice. Through determination of overexpression plant characters, compared with control group rice, overexpression shows tillering, dry weight and yield increase characters under low-nitrogen and high-nitrogen conditions, which indicates that the NIP1 gene plays an important regulation function in the aspects of tillering, dry weight, yield and nitrogen utilization of rice.

The invention provides a method for producing borneol, the method comprising culturing the transgenic microorganism in a culture medium, and the microorganism comprises or expresses borneol diphosphate synthetase and phosphatase.

The invention discloses a construction method and application of a mouse model for specifically tracing heart valve cells, and belongs to the technical field of animal model construction. The model construction method comprises the following steps: (1) constructing a Wid1-Cre hybrid mouse before inserting a Cre recombinase coding gene into a termination codon of a mouse Wid1 gene; and (2) hybridizing the Wid1-Cre hybrid mouse with the Rosa26-tdTomato homozygous report mouse, so as to obtain the double transgenic mouse which simultaneously carries the Wid1-Cre gene and the Rosa26-tdTomato gene. According to the invention, on the basis of the expression characteristic that Wif1 is only limited to a valve area in the heart, Wif1-Cre is constructed; according to the Rosa26-tdTomato mouse model, specific tracing of heart valve cells is achieved, and an important experimental tool is provided for mechanism research of heart valve diseases and development of therapeutic drugs.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The invention relates to the technical field of life science, in particular to a method for constructing an atrophic gastritis-based SPEM lesion model, which comprises the following steps: S1, constructing an Slc7a11 conditional knockout mouse strain: carrying out cage hybridization on an Slc7a11 flox / flox mouse and an ATP4b-Cre transgenic mouse, and knocking out a gastric wall cell specific Slc7a11 gene; s2, carrying out genotyping identification; the identification method based on the atrophic gastritis SPEM lesion model and the construction method based on the atrophic gastritis SPEM lesion model comprise the following steps: S1, pathological model verification: taking a mouse gastric mucosa tissue slice, quantitatively analyzing the proportion of an SPEM region by adopting an immunofluorescence double-standard technology, and confirming Slc7a11 knockout induced SPEM pathological characteristics in combination with the existence of SPEM in the gastric mucosa; and S2, long-term pathology evolution monitoring. Through gastric wall cell specific Slc7a11 gene knockout and multiple PCR identification technologies, the initial response SPEM state after gastric mucosa damage is successfully simulated, and the kit is used for researching atrophic gastritis pathological mechanisms and evaluating drug intervention.

The invention relates to cloning of a stress-resistant gene LpbHLH75 of Lilium pumilum DC (Lilium pumilum DC.) and application of the stress-resistant gene LpbHLH75 of Lilium pumilum DC. The lilium tenuifolium stress resistance key genes are mined through transcriptome data analysis, and the functions of the lilium tenuifolium stress resistance key genes are studied. Comprising the following steps: (1) cloning a target gene; (2) constructing a plant overexpression vector; and (3) detecting physiological indexes of the transgenic plant. According to the experiment, lilium tenuifolium is taken as a research object, and the LpbHLH75 gene is cloned by applying an RT-PCR technology; transforming tobacco by constructing an overexpression vector; the function of overexpression of the LpbHLH75 on tobacco salt and alkali and drought stress is identified through transgenic tobacco, the function of the lilium tenuifolium bHLH gene under adversity stress is disclosed, a new gene source is provided for lilium tenuifolium genetic engineering breeding, a reference is provided for later lilium tenuifolium stress-resistant germplasm resource research, and the lilium tenuifolium bHLH gene is developed. And a foundation is laid for exploring a gene capable of enhancing stress resistance in the lilium tenuifolium and a stress resistance mechanism of the gene.

The invention belongs to the field of antibody development, and discloses a screening method and application of a human-mouse protein high homologous target antibody based on a fully humanized antibody mouse. Aiming at the problem of weak antibody response caused by immune tolerance of human-mouse high homologous targets (protein homology is greater than or equal to 95%), the following scheme is provided: in embryonic stem cells (ES cells) of HUGO-Mabfully humanized antibody transgenic mice, a mouse target gene (such as ACVR2A) is knocked out through a Turbo Knockout technology, and homozygous knockout ES clones are screened; carrying out microinjection on the clones to the whitened B6 mouse blastocyst, and transplanting a pregnant mouse to obtain a Founder mouse; the Founder mouse is subjected to target antigen immunization for more than or equal to 4 times (the Freund's complete adjuvant is used for the first time), and the titer of the serum antibody is detected. According to the invention, 100% homozygous knockout chimeric efficiency is realized in the Founder stage, the mouse construction period is shortened from traditional 8-10 months to 3-4 months, and the diversity and affinity of the antibody are significantly improved (titer reaches 1: 729,000). The obtained antibody can be used for preparing medicines for treating tumors or autoimmune diseases.

The invention discloses a herbicide-resistant protein GAT hybridoma cell strain, an antibody generated by the herbicide-resistant protein GAT hybridoma cell strain and an application of the herbicide-resistant protein GAT hybridoma cell strain. The herbicide-resistant protein GAT hybridoma cell strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.46333. The preparation method comprises the following steps: a) purifying prokaryotic expression to obtain GAT recombinant protein; b) immunizing animals: immunizing BALB / c mice by taking the GAT recombinant protein as an antigen; c) cell fusion: splenocytes of the immune BALB / c mice are collected and fused with SP2 / 0 cells; and d) cell strain establishment: carrying out subcloning through a limited dilution method, carrying out ELISA detection after 5-7 days of subcloning until a hybridoma cell strain capable of stably secreting a positive antibody is screened out, and carrying out amplification, re-culture and storage. The monoclonal antibody secreted by the hybridoma cell strain lays a foundation for detection of herbicide-resistant protein GAT in transgenic crops, so that development, seed production and seed conservation of transgenic gat crops are supported, and stable industrial promotion of the transgenic gat crops and sustainable application of related transformants are guaranteed.

The invention provides a salt-tolerant gene, namely a PrABC1 gene and application thereof, and also provides a vector containing the gene, an expression cassette, a recombinant cell, a transgenic plant and a method for producing the transgenic plant. Specifically, the gene has a nucleotide sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 2. The plant growth regulator can be used for improving the tolerance of plants to a salt stress environment, relieving the adverse effects of a high-salt environment on the germination rate and root length of the plants, reducing the water loss rate of the plants under the high-salt stress condition and reducing the reduction of the chlorophyll content.

The invention relates to a wheat stem rot regulatory gene Tatrx-m, and an encoding protein, a recombinant vector and an application of the wheat stem rot regulatory gene Tatrx-m. The genomic sequence of the gene is SEQ ID NO.1, the CDS sequence of the gene is SEQ ID NO.2, and thioredoxin shown as SEQ ID NO.3 is encoded. The invention further provides a specific primer pair used for gene identification, a silent vector (Tatrx-m-VIGS) and an overexpression vector (LGY-OE3-Tatrx-m) are constructed, and the function of the primer pair is verified through a virus-induced gene silencing (VIGS) technology and agrobacterium-mediated transformation of wheat. Experiments show that silence of Tatrx-m results in significant reduction of wheat stem rot resistance, and overexpression of the gene can improve resistance. Based on hypha quantification, DAB staining, H2O2 content and cell death analysis, Tatrx-m is revealed to enhance wheat resistance by regulating active oxygen removal and cell wall strengthening pathways. The gene TaTrx-m has the positive regulation effect in wheat stem rot for the first time, can be applied to disease-resistant molecular marker development, gene editing and transgenic breeding, provides core genetic resources for disease-resistant variety breeding, and has theoretical and application values.

The invention discloses a gynostemma pentaphyllum transcription factor GpMYC65 and application thereof. The nucleotide sequence of the gynostemma pentaphyllum transcription factor GpMYC65 is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of the gynostemma pentaphyllum transcription factor GpMYC65. The amino acid sequence of the protein encoded by the gynostemma pentaphylla transcription factor GpMYC65 is as shown in SEQ ID NO. 2. The heavy recombinant vector contains a gynostemma pentaphylla transcription factor GpMYC65. The invention relates to application of a Gynostemma pentaphyllum transcription factor GpMYC65 in regulating and controlling the content of dammarane type triterpenoid saponin in Gynostemma pentaphyllum. The application comprises the following steps: step 1, constructing an overexpression vector containing a transcription factor GpMYC65 encoding gene; 2, transforming the overexpression vector into gynostemma pentaphyllum leaves by using an agrobacterium-mediated method to obtain genetic transformation hairy roots, and screening and identifying positive hairy roots of an overexpression transcription factor GpMYC65; and 3, carrying out liquid culture on the screened positive hairy roots to obtain transgenic hairy roots of which the dammarane type triterpenoid saponin content is higher than that of the wild hairy roots. The content of dammarane type triterpenoid saponin can be remarkably increased by overexpressing the transcription factor GpMYC65 in the fiveleaf gynostemma herb hairy roots.

The invention discloses an effect protein E56, a coding gene and application of the effect protein E56 to improvement of southern rust resistance of corn, and belongs to the technical field of agricultural biology. The amino acid sequence of the effector protein E56 is as shown in SEQ ID NO. 1. A reverse genetics method is utilized to analyze the Puccinia polystachys effect protein E56 gene, and the E56 gene is up-regulated in expression in the Puccinia polystachys infection process. The E56 gene is silenced by adopting a host-mediated gene silencing technology, and the toxic function of the E56 gene in the infection process of the southern rust disease is determined. A specific fragment of the E56 gene is cloned to an RNAi interference vector, a corn immature embryo is transformed by using an agrobacterium tumefaciens-mediated transgenic technology, and an obtained transgenic corn plant shows resistance to the corn southern rust disease. The E56-RNAi transgenic plant shows resistance to puccinia polypoda, so that the effect protein E56 can be used for creating a new strain for resisting the southern rust disease.

The invention relates to the technical field of molecular genetics, and particularly discloses a Yr6 gene, protein, a recombinant vector and application of the Yr6 gene, the protein and the recombinant vector, the full-length sequence of the Yr6 gene is shown as SEQ ID NO.1, and the CDS sequence of the Yr6 gene is shown as SEQ ID NO.2. Yr6 is overexpressed in a transgenic mode to improve wheat stripe rust resistance. The Yr6 gene provided by the invention is beneficial to improving the resistance of wheat to stripe rust, and provides support for breeding of stripe-rust-resistant wheat.

There is disclosed a mutant, non-naturally occurring or transgenic tobacco plant or part thereof having modulated expression or activity of lysine ketoglutarate reductase (LKR) comprising, consisting of, or consisting essentially of: (i) a polynucleotide, the present invention relates to a protein comprising, consisting or consisting essentially of a sequence having at least 88% sequence identity to SEQ ID NO: 1 (NtLKR-S) and / or a sequence having at least 86% sequence identity to SEQ ID NO: 3 (NtLKR-T); (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide comprising, consisting or consisting essentially of a sequence having at least 89% sequence identity to SEQ ID NO: 2 (NtLKR-S) and / or a sequence having at least 88% sequence identity to SEQ ID NO: 4 (NtLKR-T); or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i) wherein the plant or part thereof comprises at least one modification as compared to a control plant or part thereof wherein the expression of the polynucleotide or the activity of the polypeptide is not modified, the at least one modification is capable of modulating: (a) expression of the polynucleotide in the plant or part thereof; or (b) the activity of the polypeptide in the plant or part thereof.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of a BnaWLT8 gene to improvement of waterflooding stress resistance of rape. The nucleotide sequence of the BnaWLT8 gene is as shown in SEQ ID NO. 1. The oilseed rape waterlogging resistance related gene BnaWLT8 provided by the invention provides an excellent gene resource and a new effective way for improving the waterlogging stress resistance of oilseed rape and cultivating a new variety of waterlogging-resistant brassica napus. Through transgenic genetic breeding, the expression level of the BnaWLT8 gene is enhanced, the waterlogging resistance and flooding resistance of the rape in the germination stage and the seedling stage can be remarkably enhanced, and the BnaWLT8 gene has wide application prospects and good social benefits in the aspects of stress resistance improvement of the rape and the like.

The invention provides the cassava low-temperature-resistant MeSR45-2 gene cloned from cassava for the first time, the expression of the MeSR45-2 gene in cassava is inhibited under low-temperature stress, and research shows that the gene can significantly improve the low-temperature resistance of cassava, reduce the malondialdehyde content, proline cumulant and the like in cassava, reduce leaf damage under low-temperature treatment and improve the yield of cassava. Terminal buds under low-temperature treatment are not affected and can normally grow, and expression of various protein genes is regulated and controlled. The invention provides a new candidate gene for research on improvement of plant low temperature resistance and the like, and also provides a new gene resource for cultivation of a new variety of low temperature resistant cassava and construction of a new variety of transgenic cassava.

A transgenic soybean event, Gm_CSM63717, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gm_CSM63717 are also provided. Recombinant DNA molecules unique to the event Gm_CSM63717, and methods of using and detecting Gm_CSM63717 are also provided. Soybean plants containing the event Gm_CSM63717 exhibit tolerance to PPO inhibitors.

The invention discloses application of SmCLE mature peptide in inhibiting plant aging, and belongs to the technical field of plant biology. The invention discloses application of a SmCLE mature peptide in inhibiting plant aging. The amino acid sequence of the SmCLE mature peptide is SEQ ID NO: 1. The CLE peptide is applied to the aspect of inhibiting plant aging, solves the problem that the existing CLE peptide cannot be applied to anti-aging of plants, especially medicinal plants or non-mode crops, has the advantages of small molecular weight, easiness in synthesis, low action concentration and good biocompatibility, can realize accurate regulation and control of leaf aging through exogenous application, and has a wide application prospect. And the safety problem of transgenic organisms is avoided.

Disclosed herein are non-human animals (e.g., rodents, e.g., mice or rats) genetically engineered to express a human or humanized T cell receptor (TCR) from a human or humanized TCR locus comprising a non-human TCR non-coding sequence, and optionally a humanized T cell co-receptor (e.g., humanized CD4 and / or CD8 (e.g., CD8α and / or CD8β)), and / or a human or humanized major histocompatibility complex that binds the humanized T cell co-receptor (e.g., human or humanized MHC II (e.g., MHC II α and / or MHC II β chains) and / or MHC I (e.g., MHC Iα) respectively, and optionally human or humanized β2 microglobulin). Also provided are embryos, tissues, and cells expressing the same. Methods for making the genetically engineered animals are also provided. Methods for using the genetically engineered animals for developing human therapeutics are also provided.

The invention relates to a method for cultivating insect-resistant transgenic Xiaoblack poplar, in particular to a method for cultivating insect-resistant transgenic Xiaoblack poplar. The invention aims to solve the problem of low insect resistance of the populus similis. The cultivation method comprises the following steps: 1, synthesizing a Cry1Ac gene, wherein the nucleotide sequence of the gene is shown as Seq ID No: 1 in a sequence table; 2, a plant expression vector pBI121-Cry1Ac expressed by the Cry1Ac gene is constructed, and the plant expression vector pBI121-Cry1Ac is constructed; 3, preparing an infection solution; 4, cultivating the Cry1Ac-transgenic small black poplar by using an agrobacterium-mediated leaf disc transformation method, the Cry1Ac-transgenic poplar is created, the average concentration of leaf Cry1Ac protein is more than 200ng / g, the insect resistance of the transgenic poplar can be improved, all fall webworms die after being fed for 5 days, and the corrected death rate reaches 100%. The method is applied to the field of molecular breeding.

The invention belongs to the technical field of microorganisms, and particularly relates to transgenic chlamydomonas capable of secreting antibacterial peptide outside cells as well as a construction method and application of the transgenic chlamydomonas. Specifically, the invention provides transgenic chlamydomonas capable of secreting antibacterial peptide outside cells. Tests prove that not only can the water quality be improved and the immunity of fishes be improved, but also the step of purifying the antibacterial peptide protein can be effectively omitted. The zebra fish experiment is carried out, and it is proved that the survival rate of the zebra fish can be increased. Meanwhile, compared with an escherichia coli and yeast expression system, the chlamydomonas reinhardtii can also be used as bait of aquatic organisms such as fishes and shrimps, can also be cultured in a closed culture pond, and has important application potential in the aquaculture industry.

The invention relates to a genetic transformation method of mulberry positive embryogenic calluses and a preparation method of the mulberry positive embryogenic calluses by researching the infection mode, infection strain, infection liquid concentration and the like of mulberry. An effective transgenic platform is provided for subsequent research of mulberry, and a solid foundation is laid for cultivation from mulberry positive calluses to positive seedlings.