564 results about "Transgenesis" patented technology

The invention discloses a GmbZIP36 protein related to aluminum resistance of plants in acid soil as well as a coding gene and application of the GmbZIP36 protein, and belongs to the technical field of bioengineering. The amino acid sequence of the GmbZIP36 protein is shown as SEQ ID NO: 2, 3 or 4, and the nucleotide sequence of the coding gene of the GmbZIP36 protein is shown as SEQ ID NO: 1. The invention finds that the expression of the GmbZIP36 gene is induced by aluminum stress, the relative root elongation and root biomass of arabidopsis thaliana and soybean under the aluminum stress can be obviously improved by over-expressing the gene in a plant, and aluminum accumulation is reduced, so that the aluminum resistance of the plant is enhanced. The invention provides a recombinant vector containing the gene, a host cell and application of the recombinant vector and the host cell in regulating and controlling the aluminum resistance of plants. Meanwhile, the invention also provides a method for producing an aluminum-resistant transgenic plant by using the gene, a kit for detecting the gene or protein and a method for carrying out assisted breeding by using the molecular marker linked with the gene. The invention provides a key gene resource for aluminum-resistant molecular breeding of crops, and has important significance for cultivating new varieties of crops adapting to acid soil.

The invention belongs to the technical field of gene engineering, and discloses a gene for regulating tillering, dry weight and yield of rice and application of the gene. The gene is an NIP1 gene and is located on the sixth chromosome of rice, the nucleotide sequence of the NIP1 gene is as shown in SEQ ID NO.1, and the amino acid sequence coded by the NIP1 gene is as shown in SEQ ID NO.2. The NIP1 gene is cloned, a transgenic vector is constructed, an NIP1 overexpression plant is obtained, and the NIP1 overexpression plant can regulate and control tillering, dry weight and yield of rice. Through determination of overexpression plant characters, compared with control group rice, overexpression shows tillering, dry weight and yield increase characters under low-nitrogen and high-nitrogen conditions, which indicates that the NIP1 gene plays an important regulation function in the aspects of tillering, dry weight, yield and nitrogen utilization of rice.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of a BnaWLT8 gene to improvement of waterflooding stress resistance of rape. The nucleotide sequence of the BnaWLT8 gene is as shown in SEQ ID NO. 1. The oilseed rape waterlogging resistance related gene BnaWLT8 provided by the invention provides an excellent gene resource and a new effective way for improving the waterlogging stress resistance of oilseed rape and cultivating a new variety of waterlogging-resistant brassica napus. Through transgenic genetic breeding, the expression level of the BnaWLT8 gene is enhanced, the waterlogging resistance and flooding resistance of the rape in the germination stage and the seedling stage can be remarkably enhanced, and the BnaWLT8 gene has wide application prospects and good social benefits in the aspects of stress resistance improvement of the rape and the like.

The invention discloses application of SmCLE mature peptide in inhibiting plant aging, and belongs to the technical field of plant biology. The invention discloses application of a SmCLE mature peptide in inhibiting plant aging. The amino acid sequence of the SmCLE mature peptide is SEQ ID NO: 1. The CLE peptide is applied to the aspect of inhibiting plant aging, solves the problem that the existing CLE peptide cannot be applied to anti-aging of plants, especially medicinal plants or non-mode crops, has the advantages of small molecular weight, easiness in synthesis, low action concentration and good biocompatibility, can realize accurate regulation and control of leaf aging through exogenous application, and has a wide application prospect. And the safety problem of transgenic organisms is avoided.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention belongs to the field of biology, and particularly relates to a rhododendron molle heat shock protein RmHSP20-4 gene and application thereof. The RmHSP20-4 participates in response of rhododendron molle to high-temperature stress, and can be used for screening high-temperature tolerance germplasm of rhododendron molle and evaluating high-temperature tolerance of sheep. The gene is transferred into a prokaryotic expression strain, the growth of the transgenic strain under the stress of CdCl2 and high temperature is obviously improved, and the RmHSP20-4 gene and a protein coded by the RmHSP20-4 gene provide candidate genes for the cultivation of Rhododendron molle heavy metal resistance, high temperature stress resistance and other related varieties. A new candidate gene resource is provided for cultivating transgenic plants or microorganisms with excellent shapes such as heavy metal resistance and high temperature stress resistance, and an important reference is also provided for mining heat-resistant genes of azaleaceae plants.

This invention discloses a method for constructing a biological model of tertiary lymphoid structures, which can rapidly and efficiently induce the generation of in situ TLS, without relying on specific tissues or organs, and without the need for complex and expensive preparation processes such as cell editing and transgenic mice. It can generate stable, mature, and long-lasting TLS structures within 2 to 3 weeks, and has the characteristics of short cycle, low cost, simple operation, reproducibility, safety and effectiveness.

The invention discloses a gene PtoXTH30 capable of improving drought resistance and salt resistance of poplar and application of the gene PtoXTH30, the nucleotide sequence of the gene is shown as SEQ ID NO.2, the gene can accurately regulate and control the drought resistance and salt resistance of the poplar, and a key candidate gene is provided for stress-resistant molecular breeding of the poplar. The invention further discloses application of the PtoXTH30 gene for improving drought resistance and salt resistance of the poplar, and the drought resistance and salt resistance of the poplar can be remarkably enhanced. The invention further discloses a method for regulating and controlling drought resistance and salt resistance of the poplar, the drought resistance and salt resistance of the poplar can be accurately regulated and controlled according to needs, the breeding period is effectively shortened, and breeding of new drought-resistant and salt-resistant transgenic varieties is facilitated.

The invention discloses application of a TaSPL18-5D protein and a related biological material thereof to regulation and control of the ear length and the yield of a plant. According to the invention, a TaSPL18-5D overexpression wheat strain is obtained through a transgenic technology, and then analysis on the TaSPL18-5D overexpression wheat strain finds that compared with wild type wheat KN199, the TaSPL18-5D overexpression wheat strain has the advantages that the ear length is shortened, and the spikelet number and the ear grain number are obviously reduced. Therefore, the TaSPL18-5D protein can negatively regulate the ear length, the spikelet number and the grain number per ear of the plant, a new target is provided for improving the plant type and the yield of the wheat after green revolution, and the TaSPL18-5D protein has important significance for cultivating high-yield wheat varieties in agricultural production.

The invention relates to the technical field of gene engineering, in particular to a transgenic zebrafish system pTo12-efla-tjp1a-P2A-mCherry based on a Tol2 transposition subsystem and a construction method of the transgenic zebrafish system pTo12-efla-tjp1a-P2A-mCherry. The preparation method comprises the following steps: S1, constructing a Tol2 transposon expression vector pTol2-ef1a-tjp1a-P2A-mCherry which contains an ef1a promoter, a tjp1a gene, a P2A peptide sequence and an mCherry reporter gene; s2, carrying out in-vitro transcription to prepare Tol2 transposase mRNA; s3, mixing the expression vector with Tol2 transposase mRNA, and microinjecting the mixture into the single-cell stage embryo of the zebra fish; s4, performing fluorescence screening on the F0 generation embryos surviving after injection to obtain the Founder fish with positive transgenosis; s5, after the F0-generation positive fish is bred to be sexually mature, an F1 generation is obtained through mating, transgenic positive individuals with stable inheritance are screened out after identification, and a transgenic zebrafish strain is established. According to the transgenic zebrafish system pTo12-efla-tjp1a-P2A-mCherry based on the Tol2 transposon system and the construction method of the transgenic zebrafish system pTo12-efla-tjp1a-P2A-mCherry based on the Tol2 transposon system, efficient integration of exogenous genes is achieved by utilizing the Tol2 transposon system, and the transgenic zebrafish line capable of being stably inherited to the F1 generation is successfully obtained.

The invention provides an application of a rape RPM1 interaction protein BnaRIN4 in regulating and controlling the oil content of rape seeds. On the basis of whole genome association analysis and whole transcriptome association analysis, a candidate gene BnaRIN4 for regulating and controlling the oil content is predicted, a homozygous mutant material with deletion of the gene BnaRIN4 function is further created through a transgenic technology, and the gene plays an important role in negative regulation and control of the oil content of the rape seeds, so that the application of the gene in negative regulation and control of the oil content of the rape seeds is realized. By specifically knocking out or mutating the gene, a novel rape germplasm of which the oil content of the seeds is remarkably improved can be obtained. The invention discloses the biological function of the gene in regulating and controlling the oil content of rape seeds for the first time, and provides theoretical basis and technical support for molecular breeding of high-oil-content rape varieties.

The invention discloses application of a brassica juncea gene BjuABCG42-A03 in regulating and controlling pollen germination and seed setting amount of brassica juncea, and relates to the technical field of plant genetic engineering, the amino acid sequence of the protein BjuABCG42-A03 is as shown in SEQ ID No.1, and meanwhile, the invention provides a method for cultivating a transgenic plant capable of improving the pollen germination rate or seed setting amount. The method comprises the following steps: constructing a recombinant plant expression vector containing a gene for coding protein BjuABCG42-A03; the constructed recombinant plant expression vector is transformed into agrobacterium and then transformed into a plant or a plant cell, and the gene of the coded protein BjuABCG42-A03 is over-expressed in the plant, so that the pollen germination rate or the seed setting amount of the plant is increased. Based on gene information of arabidopsis ABCG42 (At4g15233), a BjuABCG42-A03 gene is homologous cloned in a mustard genome, in-vivo and in-vitro germination experiments show that the BjuABCG42-A03 gene affects the elongation length and seed bearing quantity of a pollen tube, and a research direction is provided for solving the problem of low pollen fertility or low seed setting quantity of mustard subsequently.

The invention belongs to the technical field of agricultural biology, and particularly relates to a plant constitutive promoter OsSULTR2. 2pro and an application thereof. The promoter is characterized in that the promoter is OsSULTR2; the nucleotide sequence of the 2pro is as shown in SEQ ID NO. 1. The promoter provided by the invention is OsSULTR2; 2pro is a rice endogenous constitutive promoter, can drive a target gene to realize efficient and stable expression in plant callus, roots, stems, leaves, young ears, seeds and other tissues in a vegetative growth period, and can replace an existing non-plant-source promoter. The method has important application value in the field of plant genetic engineering, and can effectively reduce potential safety risks of transgenic plants caused by introduction of exogenous DNA.

The invention discloses application of an OsGIT2 gene in rice blast resistance of rice. The applicant constructs a CRISPR carrier of the gene and transforms a japonica rice variety Kitaake of rice to obtain a transgenic line material, and the resistance of the transgenic line material to magnaporthe oryzae is obviously higher than that of a wild type material. The mutant rice is obtained by an applicant through a CRISPR-Cas9 technology, the mutant shows resistance to rice blast, and the technical scheme of the invention is of great significance to creation of broad-spectrum disease-resistant high-yield excellent new germplasm.

This invention belongs to the field of molecular genetics and discloses a method and application for controlling fish fertility using in vivo gene editing technology. The applicant, for the first time, cloned a carp-specific U6 promoter and used this promoter to construct the U6 gene. MOVIE gRNA transgenic vectors and water The Cas9 vector allows for the establishment of an in vivo editing system. This strategy enables the heritability of sterility, a reproductive control trait, through hybridization of fertile parents to produce sterile offspring. The operation is highly efficient, simple, and environmentally friendly. Furthermore, since high-copy-rate short, scattered repetitive sequences exist in all fish species, this strategy has broad applicability across species.

The invention relates to the technical field of plant breeding, in particular to a primer combination for detecting a regulatory element and an exogenous gene of a transgenic plant and application of the primer combination. The primer combination comprises a nucleotide sequence as shown in SEQ ID NO. 22-63. The primer combination comprises a nucleotide sequence as shown in SEQ ID NO. The application comprises: (1) transgenic plant variety germplasm supervision or transgenic plant component screening in non-transgenic varieties; (2) screening transgenic ingredients of the plant product; (3) auxiliary screening in the plant breeding process; and (4) tracing plant varieties. The invention provides a primer combination which can be applied to detection of regulatory elements and exogenous genes of transgenic plants, can realize detection of a plurality of regulatory elements and exogenous genes in a reaction system, and can meet high-throughput screening of components of the transgenic plants. The method is used for monitoring illegal planting of transgenic plants and detecting transgenic components of related plant products.

The invention belongs to the technical field of biological genetic engineering, and particularly relates to a mouse model construction scheme capable of reproducing a key phenotype of hemophagocytic syndrome, which comprises the following steps: inserting a loxP-STOP-loxP-LMP1 conditional expression cassette into a Rosa26 safe port site in a fixed point manner to obtain an Lmp1cKI mouse strain; and hybridizing the mouse with an Ncr1-Cre transgenic mouse of NK cell specific expression Cre recombinase. In a double transgenic offspring (Lmp1Ncr1-Cre), STOP fragments are cut off by Cre enzyme, and continuous high expression of EB virus LMP1 protein in NK cells is driven. The model does not need chemical induction or immune intervention, and typical hemophagocytic syndrome phenotypes, namely serum IL-6, TNF-alpha and IFN-gamma, spontaneously appear within 7-9 days after young rats are born; the body weight is reduced and the survival rate is obviously reduced; the proportion of lymphocytes is sharply reduced; and NK cells are depleted. The invention provides a stable and reliable tool for hemophagocytic syndrome pathogenesis research, drug screening and treatment evaluation.

The invention relates to the technical field of animal genetic breeding and genomics, and discloses a reference genome of a chromosome level of a Zhaotong Wujin pig and application of the reference genome. The high-quality chromosome level reference genome of the Zhaotong Wujin pig is constructed for the first time. The total length of the genome is about 2.66 Gb, the chromosome anchoring rate is more than 98%, Contig N50 is about 102Mb, and the integrity evaluation of the genome is more than 98%; 21507 protein coding genes are annotated in total. Compared with the existing reference sequence, the genome disclosed by the invention contains specific sequence variation and gene family information of local pig breeds, and provides a more accurate and comprehensive genetic blueprint. The genome resource can be widely applied to molecular mechanism research related to traits such as meat quality, reproductive capacity, growth speed, disease resistance and the like, molecular marker development, breeding chip design, quantitative trait locus (QTL) positioning, genetic evaluation, gene editing, transgenic breeding, germplasm resource protection and the like. The method has important application value in genetic improvement of Chinese local pig breeds.

The present application relates to the field of biological genetic engineering technology, in particular to the application of silencing miR529b in the positive regulation of rice resistance to brown planthopper. The silencing vector of miR529b is obtained by short tandem target mimic (STTM) technology, which silences miR529b in the receptor rice, and obtains the transgenic plant STTM-miR529b with silenced miR529b. The T2 generation homozygous transgenic plants are identified for brown planthopper resistance by seedling group method. Compared with the transgenic receptor parent Nipponbare (control group, marked as NIP, same below), STTM-miR529b is more resistant to pests, which confirms that miR529b is related to the resistance of rice to brown planthopper, and can be used for creating new germplasm of rice resistance to brown planthopper.

The invention relates to a DNA (Deoxyribose Nucleic Acid) for regulating and controlling a resistance gene LTPL160 of rice bacterial blight and breeding utilization of a protein of the DNA. According to the invention, the gene OsLTPL160 for regulating and controlling the resistance of the rice bacterial leaf blight is cloned by virtue of a non-gel quantitative proteomics analysis and transcription analysis method. The OsLTPL160 knockout and overexpression material is obtained by adopting a gene editing and transgenic technology. The OsLTPL160 is knocked out, so that the bacterial blight resistance of the rice is remarkably improved; and overexpression of the OsLTPL160 obviously reduces the resistance of the bacterial blight of the rice. The cloning of the OsLTPL160 gene and the construction of the material provide material and technical support for the bacterial blight resistance of the rice.

The invention discloses an establishment method of a cherry rootstock Gisela 6 leaf in-vitro regeneration system and transgenic application thereof, and belongs to the field of plant biotechnology and genetic engineering. According to the regeneration system established by optimizing the formula of the culture medium, the regeneration frequency of the 'Gisela 6' leaf is stabilized at a relatively high level, and the quality of the regenerated material is remarkably improved. The callus generated by induction of the method provided by the invention is compact and strong in browning resistance, and the transgenic callus capable of stably expressing RUBY is obtained by a leaf disc transformation method; meanwhile, vitrification of regenerated buds is effectively inhibited, robust plants easy to transplant are obtained, and technical support is provided for precise improvement and breeding speed.

The invention discloses a construction method of a conditional knock-down dynactin animal model of astrocytes and an application of the animal model of the conditional knock-down dynactin animal model of the astrocytes. According to the method, a Dctn1LoxP gene knock-in mouse is hybridized with an Aldh1l1-Cre / ERT2 transgenic mouse, a target genotype mouse is obtained through three rounds of breeding, 100mg / kg Tamoxifen is continuously injected into the intraperitoneal cavity of the 2-month-old mouse for 5 days, and specific knock-down of dynactin in brain and spinal astrocytes is realized, including knockout of p150Glue and reduction of DCTN4, p50 and Arp1alpha protein levels. The model has the advantages of being high in specificity, permanent in intervention aging and capable of covering multiple life stages, the defects of a traditional model are overcome, the model can be used for researching the influence of dynactin on astrocyte neurobiological functions, a reliable tool is provided for screening related targets of nervous system diseases, and the model has important application value.

The invention discloses an application of an overexpressed CrOASTL1 gene in improving the soluble protein content of leguminous forage, and belongs to the technical field of biology. 1, and the CDS sequence of the CrOASTL1 gene is as shown in SEQ ID NO. 2. The method comprises the following steps: cloning a CrOASTL1 gene segment, constructing a plant overexpression vector, carrying out plant transgenosis operation, and introducing into leguminous forage plant cells to obtain a leguminous forage transgenosis line with the overexpressed CrOASTL1 gene. The CrOASTL1 gene is over-expressed in medicago sativa, and the soluble protein content of leaves of the medicago sativa is remarkably increased by about 19%. The CrOASTL1 gene of chlamydomonas reinhardtii is applied to quality improvement of leguminous forage for the first time, the technical obstacle that the genetic relationship of species is relatively far is overcome, a brand new gene resource and an efficient technical approach are provided for cultivating a new variety of leguminous forage with high protein and high nutritional value, and the method has important significance in promoting sustainable development of grass and animal husbandry.

The present disclosure relates to animal models that express human tryptophanyl-tRNA synthetase (hWARS) knock-in. These animal models are highly susceptible to enterovirus infection, including but not limited to EV-D68 and EV-A71 infection. Also disclosed are screening methods and compounds that utilize these animal models.

The invention discloses a rice laodelphax striatellus resistance gene OsSAMS1 and application thereof. The gene provided by the invention is a DNA (Deoxyribonucleic Acid) molecule as shown in SEQ ID NO.2 and SEQ ID NO.3. The invention also provides a method for preparing the Corresponding proteins coded by the gene provided by the invention are proteins as shown in (a) and (b): (a) an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is formed by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID NO.1, is related to rice laodelphax striatellus resistance and is derived from SEQ ID NO.1. The rice insect-resistant related protein provided by the invention affects the insect resistance of plants. Insect-sensitive plants can resist insects by improving expression of the protein coding gene, and laodelphax striatellus-resistant transgenic plants can be cultivated by silencing the gene. The gene and the encoding protein thereof can be applied to plant genetic improvement.

The invention relates to the technical field of biology, in particular to application of an RhUNE10 gene to regulation and control of root cancer resistance of Chinese roses and a regulation and control method. The nucleotide sequence of the RhUNE10 gene is as shown in SEQ ID NO. 1. The application mode is that the RhUNE10 gene is silenced and knocked out, or the expression of the protein coded by the RhUNE10 gene is inhibited; and the amino acid sequence of the protein coded by the RhUNE10 gene is as shown in SEQ ID NO. 3. After the RhUNE10 gene is silenced, the resistance of the Chinese rose to agrobacterium tumefaciens is remarkably reduced, which indicates that the RhUNE10 gene has important biological functions, is beneficial to promoting Chinese rose functional genomics research, gene editing breeding and transgenic breeding research, excavates and cultivates new varieties of high-resistance Chinese roses, and has wide application prospects. And a certain theoretical basis and key gene resources are provided for comprehensive prevention and treatment of root cancer and cultivation of a new variety of Chinese rose.

The application belongs to the technical field of genetic engineering, and particularly discloses AtDi19 Application of the gene in drought resistance and high-oil rape breeding AtDi19 The gene is first transferred into the rape by using the transgenic technology, and the transgenic rape obtained has significantly improved drought resistance compared with the wild type rape; and the oil content of the rape seed is significantly increased under the drought stress. Therefore, the application provides a new gene resource for drought-resistant and stable high-oil rape breeding.

The application relates to a construction method of a Mettl3 transgenic mouse model with specific knockout, enriches the understanding of the function and action mechanism of Mettl3 in mature pancreatic islet beta cells, and provides a new thought and target for the pathogenesis and treatment of diabetes.

The present invention describes the synthesis and biological evaluation of new substituted pyridine derivatives as foxj1 activity enhancers. In embodiments, novel pyridine derivatives were synthesized and the ability thereof to enhance foxj1 activity was evaluated. The novel pyridine derivatives synthesized in the present invention not only exhibited excellent activity but also exhibited excellent metabolic safety and pharmacokinetic profile in a Tg (foxj1: egfp) transgenic zebrafish animal model. In addition, the novel pyridine derivatives synthesized in the present invention enhanced cilia-promoting capability in a motile ciliated cell differentiation experiment in which mouse tracheal epithelial cells (mTECs) were isolated from the airways of mice and cultured by ALI. As a result, a series of foxj1 activity enhancers with substituted pyridine skeleton has the potential to be developed as preventive and therapeutic agents for respiratory diseases.