35 results about "Halotolerance" patented technology

The invention belongs to the technical field of saline-alkali soil improvement, and particularly relates to a coastal saline-alkali soil-oriented intelligent pH-responsive controlled-release crop growth-promoting soil conditioner as well as a preparation method and application thereof. The modifier is biochar-based composite microspheres, an inner core is formed by compounding biochar, silicon quantum dots and a nitrogen-phosphorus-potassium compound fertilizer, an outer layer is coated with a pH response coating formed by chitosan, and an integrated system of environmental perception, release regulation and accurate fertilizer supply is constructed. After the fertilizer is applied to soil, a coating layer swells under the rhizosphere micro-domain acidic condition, nutrient diffusion is intelligently regulated and controlled, and leaching loss and volatilization are reduced. The biochar porous structure adsorbs and fixes salt ions, improves aggregate and ventilation and water permeability, and enhances water retention and fertilizer conservation; the silicon quantum dots improve the photosynthetic efficiency and enhance the oxidation resistance; the compound fertilizer is continuously supplied to nitrogen, phosphorus and potassium. The components synergistically improve soil microstructure and water and salt migration, relieve salt ion poison, improve nutrient utilization and crop salt tolerance, promote yield increase and stability and are suitable for saline-alkali soil remediation.

The invention belongs to the technical field of microorganisms, and particularly relates to a salt-tolerant endophytic fungus DZ-Sp-9 and application thereof. The classification name of the salt-tolerant endophytic fungus DZ-Sp-9 is Sedecimilla taiwanensis DZ-Sp-9, the salt-tolerant endophytic fungus DZ-Sp-9 is preserved in the China Center for Type Culture Collection on August 22, 2025, and the preservation number of the salt-tolerant endophytic fungus DZ-Sp-9 is CCTCC (China Center for Type Culture Collection) NO: M 20251874. Experiments prove that the salt-tolerant endophytic fungus DZ-Sp-9 can promote the growth of corn seedlings and improve the salt tolerance of the corn seedlings, and can be developed into a microbial fertilizer in the future to be used for planting crops in saline-alkali soil.

The invention relates to the field of microorganisms, in particular to bacillus subtilis, a microbial agent and application of the microbial agent. The invention provides bacillus subtilis, and the preservation number of the bacillus subtilis is CGMCC (China General Microbiological Culture Collection Center) No.36176. The invention further provides a preparation method of the bacillus subtilis. The bacillus subtilis strain which is high in salt tolerance, stable in function and wide in application range is obtained through screening, and the bacillus subtilis strain has important theoretical significance and practical application value for improving the saline-alkali soil biological improvement effect, expanding the application scene of microbial fertilizer in saline-alkali soil and promoting ecological restoration.

Provided are a marine concrete composition using dechlorination microorganisms capable of easily removing chlorine generated by seawater through an electrical method by allowing electrons emitted from electricity-generating microorganisms to flow through steel fibers incorporated into ultra-high-performance concrete (UHPC) or high-performance fiber reinforced concrete (HPFRCC) through a dechlorination microbial capsule carrier and capable of self-healing concrete crack sites through a self-healing microbial capsule carrier and is also capable of fundamentally solving the problem of reduced durability against salt damage of ultra-high-performance concrete or high-performance fiber reinforced concrete for application in marine construction environments through a dechlorination microbial capsule carrier, and a method for constructing a marine concrete structure using the same.

The invention belongs to the technical field of saline-alkali soil improvement and agricultural biology, and particularly relates to a coastal saline-alkali soil green manure combined desalting and fertilizing method based on multistage screening. According to the method, sand culture and water culture are combined, different NaCl concentration gradients are set, the salt tolerance of green manure crops is evaluated, and green manure varieties suitable for different salinization degrees are screened out; planting the screened green manure varieties in different areas according to the salinization degree of the saline-alkali soil; after the green manure grows to a proper period, turning and returning to the field to realize soil desalination and fertilization. According to the method, multi-dimensional agronomic characters such as the emergence rate, the root length, the plant height and the fresh weight are comprehensively evaluated through a multi-stage screening method combining sand culture primary screening and water culture secondary screening, green manure varieties suitable for mild, moderate and severe salinized soil are scientifically screened out, the defects that in the prior art, green manure selection is blind, and a targeted evaluation system is lacked are overcome, and the method is suitable for large-scale popularization and application. And accurate variety support is provided for biological improvement of the coastal saline-alkali land.

The application discloses a salt-tolerant oil-degrading bacterium, a biological preparation and application of the salt-tolerant oil-degrading bacterium in oil degradation, and belongs to the technical field of biological degradation. The strain of the salt-tolerant oil-degrading bacterium is named IURM G72, is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC NO.29726. The salt-tolerant oil-degrading bacterium IURM G72 and the preparation thereof have good salt tolerance and oil-degrading performance, can grow with oil as the only carbon source in the case that the salt concentration is high, and have simple nutritional requirements. The degrading bacterium and the preparation thereof can effectively degrade oil in high-salt kitchen waste, and have important significance for water bodies and soil polluted by waste edible oil.

The invention provides paenibacillus kuchii as well as a fungicide and application thereof, and relates to the field of microorganisms. The paenibacillus kuchii is specifically paenibacillus kuchii M18, and the preservation number of the paenibacillus kuchii M18 is CGMCC (China General Microbiological Culture Collection Center) No. 36293. The strain can be used for dissolving phosphorus and potassium, fixing nitrogen and secreting IAA (Indoleacetic Acid), and is beneficial to promoting plant growth and improving soil quality; the strain can produce a biological membrane, can effectively isolate the contact between a root system and pathogenic bacteria, and plays a crucial role in preventing and treating various soil-borne diseases; the strain can produce protease and cellulase and promote the growth of plants; the strain can produce a urease inhibitor, inhibit the activity of urease in soil and delay hydrolysis of urea. The strain also shows extremely strong salt tolerance, and is beneficial to exerting a growth promoting effect in saline-alkali soil. The microbial inoculum can be applied to the root of a plant by means of root irrigation, so that the strain is colonized at the root of the plant to play a role in promoting the growth of the plant.

The invention discloses a targeted biological bacterium coupling method for comprehensive treatment of coastal vegetable salt damage, heavy metal and pesticide residues and application, and relates to the field of agricultural biotechnology and soil remediation. According to the method, bacillus subtilis, bacillus mucilaginosus, brevibacillus laterosporus, pseudomonas fluorescens, arbuscular mycorrhizal fungi and host original flora extracted from healthy vegetable rhizosphere soil are subjected to coupled symbiotic culture, and target flora with multiple functions is formed. The functional flora is compounded with a nutrient carrier containing potassium humate and fulvic acid, a seaweed extract and a specific salt-resistant additive to prepare the multifunctional water-soluble fertilizer or fungicide. After the compound fertilizer is applied to a coastal vegetable production base, the synergistic effects of degrading soil pesticide residues, passivating heavy metals, enhancing the salt tolerance of vegetables and improving the soil fertility and microbial diversity can be synchronously realized, the compound obstacle problem of coastal vegetable production is fundamentally solved, and the yield, quality and safety of vegetables are guaranteed.

The application discloses a salt-tolerant adenylate kinase mutant and application thereof. Through modification on an amino acid sequence of a wild-type adenylate kinase (adenylate kinase, EC 2.7.4.3) from Bacillus subtilis (strain 168), three salt-tolerant adenylate kinase mutants are obtained; the mutant adenylate kinase has higher salt tolerance compared with the wild-type adenylate kinase, and can tolerate a salt concentration of 300-2000 mmol / L in an adenosine diphosphate reaction system.

The invention belongs to the technical field of agriculture, and particularly relates to application of halomonas DY-S025GC05 to improvement of salt tolerance of plants and / or promotion of plant growth. The invention provides an application of halomonas DY-S025GC05 in improving the salt tolerance of plants and / or promoting the growth of the plants, the halomonas DY-S025GC05 is derived from deep-sea sediments below 3000 meters in a ninety-degree Hailing of Indian Ocean, and the strain can obviously improve the salt tolerance of the plants and promote the growth of the plants; at the low temperature (10 DEG C), the strain still can efficiently play a role in improving the growth performance and the salt tolerance of plants, so that a new source is provided for obtaining efficient functional strains for solving soil problems, and the strain has a huge application prospect in the fields of saline-alkali soil improvement and green biological fertilizers.

The application discloses a method for high-efficiency degradation of high-salinity wastewater by salt-tolerant bacteria in a composite inorganic salt system, and relates to the technical field of biological treatment of high-salinity industrial wastewater.The method realizes directional strengthening of the salt-tolerant performance of three strains of Staphylococcus xylosus, Alcaligenes faecalis and Bacillus cereus by a gradient-increasing NaCl domestication method.Under the condition of 40-50 g / L high salinity, the growth amount of the domesticated strains is increased by 30-40% compared with that of the non-domesticated strains, effectively relieving the osmotic pressure stress and metabolic enzyme activity inhibition of the microorganisms in the high-salinity environment.Meanwhile, the composite salt-tolerant bacteria agent is compounded based on functional complementation, and the agent has the abilities of efficient denitrification, phosphorus removal and organic matter degradation, and can adapt to the composite inorganic salt system of NaCl, CaCl2 and MgSO4 alone or in combination, and can realize synchronous and efficient removal of COD, ammonia nitrogen, total nitrogen and total phosphorus in a wide salinity range of 10-80 g / L.The method breaks through the technical limitation that the existing salt-tolerant bacteria only adapt to a single salt system and have single degradation function, and greatly widens the applicable scenarios of the biological method in the treatment of high-salinity wastewater.

The application discloses a salt-tolerant and polycyclic aromatic hydrocarbon-degradable Pseudomonas taiwanensis SYSU M80001. The strain is preserved in the China General Microbiological Culture Collection Center on March 3, 2023, and the preservation number is CGMCC No. 26731. The degradation efficiency of the strain to 100 mg / L phenanthrene is 88%, and the strain can tolerate high-concentration phenanthrene and has high-efficiency degradation capacity to high-concentration phenanthrene. Meanwhile, the strain also has good salt tolerance, and can tolerate 5% salinity at most, and has good phenanthrene degradation performance under 0-4% salinity, and the phenanthrene degradation efficiency under 3% salinity is the highest. In addition, the strain also has degradation potential to naphthalene, anthracene and other polycyclic aromatic hydrocarbons. It is shown that the Pseudomonas taiwanensis SYSU M80001 screened in the application can efficiently degrade polycyclic aromatic hydrocarbons, and is especially suitable for PAHs pollution remediation in high-salinity environments such as estuaries.

The invention provides a method for increasing salt tolerance of zebrafish through a gene knockout technology and application, belongs to the technical field of gene engineering and molecular breeding, utilizes a gene editing technology and provides proper gRNA, a circpb gene can be directly knocked out, the zebrafish with salt tolerance is obtained, and the method is expected to be popularized to economic fish.

The invention relates to the field of material science and engineering, and particularly discloses an application method of a drought-tolerant and salt-tolerant field plant water-retaining agent. The water-retaining agent is prepared from acrylic acid, acrylamide, sodium carboxymethyl cellulose, humic acid, a cross-linking agent and an initiator through graft copolymerization. The introduction of humic acid constructs an ion buffer network, and significantly improves the imbibition rate and water retention durability of the product in a saline-alkali environment. The invention also adopts an application method of the water-retaining agent in saline-alkali soil, and the key point is that the water-retaining agent is mixed into a 15-20cm root activity layer through broadcast application and deep ploughing, or the water-retaining agent is covered with soil with the thickness of 3-5cm to serve as an isolating layer during hole application. According to the application method, direct contact between dry glue and the root system is blocked through physical isolation, and the root burning phenomenon caused by reverse water absorption is effectively avoided. The salt tolerance and the application safety of the product are synergistically improved, and the transplanting survival rate and the biomass of plants in the arid and saline-alkali areas are remarkably improved.

The invention discloses bacillus cereus and application thereof in salt tolerance and growth promotion of plants, and belongs to the technical field of microorganisms. The bacillus cereus CZJ1 capable of promoting the plant growth and improving the salt stress resistance of the plant is screened and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36251. The bacillus cereus CZJ1 has the advantages that the bacillus cereus CZJ1 can promote the plant growth and improve the salt stress resistance of the plant; experimental results show that the bacillus cereus CZJ1 has the salt-tolerant characteristic and the functions of fixing nitrogen, dissolving phosphorus, dissolving silicate, producing iron carriers and the like, and can effectively promote absorption and utilization of plants to elements such as nitrogen, phosphorus, iron and the like, so that plant growth is promoted. The bacillus cereus CZJ1 has an obvious improvement effect on the growth of wild barley plants under salt stress and the salt stress tolerance of the wild barley plants. The invention provides a new growth-promoting bacterium for improving the salt tolerance of plants, and provides a new technical means for planting crops in saline-alkali soil.

The invention relates to the technical field of soil improvement. The invention provides a saline-alkali soil improving microbial agent and a preparation method thereof. The saline-alkali soil improving microbial agent is prepared from the following raw materials in parts by weight: 15 to 20 parts of bacillus megatherium, 10 to 15 parts of bacillus subtilis, 8 to 12 parts of bacillus mucilaginosus, 5 to 8 parts of pseudomonas fluorescens, 6 to 10 parts of trichoderma harzianum, 5 to 8 parts of arbuscular mycorrhizal fungi and 3 to 5 parts of rhizobium. Through cooperative use of the strains, the salt content of the coastal salinized soil can be effectively reduced, the pH value can be adjusted, the nutrient index of the salinized soil can be improved, the content of microorganisms in the soil can be improved, and the root development of crops can be promoted. Meanwhile, the sectional fermentation process can solve the problem of strain antagonism, and the salt tolerance and viable count of the strain are improved in cooperation with a special composite culture medium, so that the saline-alkali soil improvement effect is further enhanced.

The invention provides a salt-tolerant bacillus, a microbial agent and application. The salt-tolerant bacillus has excellent salt tolerance, can stably survive and breed in a high-salt environment, and can activate soil nutrients, improve soil fertility, promote decomposition of organic matters of plants and provide sufficient nutrition sources for plant growth through the effects of nitrogen fixation, potassium decomposition, sulfur decomposition and the like; meanwhile, the strain or the microbial agent can enhance the stress resistance of the plants, help the plants to resist adverse environmental influence and improve the growth state; in the aspect of a biocontrol function, the salt-tolerant bacillus and the microbial agent have a remarkable inhibition effect on plant fungal diseases, and the survival rate, the biomass and the yield of crops are improved.

This invention discloses a Gobi abnormal coccus ( Deinococcus gobiensis Genes encoding ferritin in I-0 DgfE2 The nucleotide sequence of this gene is shown in SEQ ID NO. 1. The protein DgfE2 encoded by this gene has salt-tolerant function and can be used to cultivate salt-tolerant microorganisms, thus providing a reference for cultivating plants with salt-tolerant traits. The amino acid sequence of this protein is shown in SEQ ID NO. 2. After being subjected to 0.6 mol / L NaCl stress for 5 h, it contains... DgfE2 Salt-tolerant recombinant strain pET28a- DgfE2 The growth of the / BL21 strain was better than that of the control pET28a / BL21 strain containing only an empty plasmid, indicating that this recombinant strain has the ability to tolerate salt stress. DgfE2 DgfE2 The gene has the function of improving the salt tolerance of E. coli.

The application discloses a bacillus megaterium strain for increasing adversity tolerance of aquatic animals and application thereof. The bacillus megaterium strain is named bacillus megaterium SCAU-Ohb1, the strain has been preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO.62119, and the preservation date is December 13, 2021. The bacillus megaterium SCAU-Ohb1 is isolated from fish intestinal tracts and is safe to fish bodies. After the fermentation liquor of the bacillus megaterium SCAU-Ohb1 is mixed with a basic daily ration and is used for feeding freshwater fish, the activity of important antioxidant enzymes in the fish bodies can be significantly improved, the salt tolerance of gill tissues of the freshwater fish under acute salt stress can be increased, and the survival rate of the fish bodies can be improved. The application provides a new method for improving adversity tolerance of aquatic animals, has important significance for the aquaculture industry, and is expected to improve production efficiency and reduce losses.

The present disclosure relates to the use of a composition for improving the tolerance of a plant or algae to salt, comprising a molecule, wherein the molecule inhibits an expression of a specific nucleotide sequence. Furthermore, the present disclosure relates to a method and kit to determining the tolerance of a plant or algae to salt.

The invention discloses bacillus atrophaeus and application thereof in salt tolerance and growth promotion of plants, and belongs to the technical field of microorganisms. The preservation number of the bacillus atrophaeus is CGMCC (China General Microbiological Culture Collection Center) Experimental results show that the bacillus atrophaeus provided by the invention has the salt-tolerant characteristic and the functions of fixing nitrogen, dissolving phosphorus, dissolving silicate, producing siderophores and the like, and can effectively promote absorption and utilization of plants to elements such as nitrogen, phosphorus, iron and the like so as to promote plant growth. The bacillus atrophaeus has an obvious improvement effect on the growth of wild barley plants under the stress of high-concentration salt and the endurance capacity of the wild barley plants, and can also remove heavy metals in high-salt wastewater. The invention provides a new growth-promoting bacterium for improving the high salt tolerance of plants, and provides a new technical means for high-concentration saline-alkali soil crop planting and wastewater heavy metal pollution treatment.

The utility model provides a kind of coupling temperature control device and salt-tolerant artificial wetland system, the system includes water inlet channel, filler pool and water outlet channel in turn, the filler pool includes pool body of four around and bottom and filler structure inside pool body, the filler structure is multilayer composite structure, from bottom to top in turn is temperature control layer, salt-tolerant sandy filler layer, salt-tolerant microorganism immobilization carrier layer and salt-tolerant plant planting layer;The temperature control layer is embedded with geothermal circulation pipe and solar energy heat collection pipe;The salt-tolerant sandy filler layer is divided into three layers from bottom to top in the order of filler particle size from big to small, and wave-shaped guide vane is equipped between each layer;The salt-tolerant microorganism immobilization carrier layer is formed by embedding carrier with salt-tolerant microorganism, and magnetic biochar module is also embedded therein;Salt-tolerant plant is also planted on the salt-tolerant plant planting layer;The utility model realizes the efficient, stable treatment of industrial wastewater plant salt-containing tail water in northern cold region, conducive to promoting the achievement of resource utilization and low-carbon target.

The invention discloses a sophorolipid composition for a slickwater fracturing fluid as well as a preparation method and application of the sophorolipid composition, and relates to the technical field of slickwater fracturing fluids. The main component of the sophorolipid composition for the slick water fracturing fluid is sophorolipid produced by fermentation of Candida bombycla, and the sophorolipid composition also comprises a thickening agent, a drag reducer and a clay stabilizer. The slick water fracturing fluid is simple in preparation method and low in use cost, main active ingredients are biologically sourced, and drag reduction, temperature and salt resistance, emulsifying activity and environmental protection requirements are considered, so that the drag reduction rate is increased, the recovery efficiency is increased, and the slick water fracturing fluid has a wide application prospect in oil field hydraulic fracturing.

The application provides a stone cultural relic crack grouting method based on a high-pH-resistant microbial mineralization process. The method comprises small-scale crack and large-scale crack grouting repair; the small-scale crack is treated by grouting with a microbial slurry, and the microbial slurry is prepared from urea, a high-pH-resistant microbial liquid and hydraulic lime; the large-scale crack is sealed and protected by hot melt adhesive and paper adhesive tape before grouting, grouted by microbial mortar prepared from stone powder, hydraulic lime, urea and the high-pH-resistant microbial liquid, and subjected to surface shaping treatment after the grouting and curing are completed; and the high-pH-resistant microbial liquid is obtained by domesticating non-toxic and harmless microorganisms. The application provides a technology and method suitable for cracks of stone cultural relics of multiple scales, can improve the early strength of the hydraulic lime, has the advantages of good salt resistance, green environmental protection, simple operation, low cost, wide application range and the like.

The utility model discloses a heterotrophic denitrification device and a heterotrophic denitrification system for deep denitrification of petrochemical high-salinity wastewater, aims to solve the problem of inhibition of a high-salinity environment on a heterotrophic denitrification nitrogen removal process, and provides an efficient and stable wastewater nitrogen removal solution. The device mainly comprises a salt-tolerant denitrifying flora immobilization system, a carbon source slow-release module and a reaction parameter monitoring system. The biological stability of the salt-tolerant heterotrophic denitrifying flora in a high-salt environment is improved through an immobilization technology, and sufficient nutrition supply is provided through a carbon source slow release system. The online monitoring system monitors key parameters such as pH value, dissolved oxygen, oxidation-reduction potential, salinity and pressure difference in real time to ensure optimal reaction conditions. Compared with the prior art, the device disclosed by the utility model can be used for efficiently and stably removing nitrogen pollutants in the wastewater in a high-salinity environment, is relatively low in operation cost and is widely applied to deep denitrification of high-salinity wastewater in industries such as petrochemical industry and the like.

The invention discloses paracoccus martensii, a saline-alkaline tolerant growth-promoting microbial agent and application thereof, and belongs to the technical field of microorganisms. The paracoccus martensii CZJ5 capable of promoting the plant growth and improving the salt stress resistance of the plant is screened out, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36255. Experimental results show that the paracoccus martensii CZJ5 has the salt-tolerant characteristic and the functions of nitrogen fixation, phosphorus dissolution, silicate removal, siderophore production and the like, and can effectively promote absorption and utilization of plants to elements such as nitrogen, phosphorus and iron, so that plant growth is promoted. The Paracoccus martensii CZJ5 has an obvious improvement effect on the growth of wild barley plants under salt stress and the salt stress tolerance of the wild barley plants. The invention provides a new growth-promoting bacterium for improving the salt tolerance of plants, and provides a new technical means for planting crops in saline-alkali soil.

The invention discloses low-temperature salt-tolerant hydrolase sourced from marine metagenome and capable of efficiently degrading PET (Polyethylene Terephthalate) plastic and application thereof. The hydrolase NH937 can degrade polyethylene glycol terephthalate (PET) at the temperature of 40 DEG C, the catalytic efficiency of the hydrolase NH937 is dozens of times higher than that of reported IsPETase, and the hydrolase NH937 has remarkable PET degradation activity in a 0-5.3 M NaCl buffer solution with the wide PH range of 5.0-11.0 and natural seawater and is a PET hydrolase with high salt resistance in a low-temperature environment at present. The NH937 can change the surface morphology of PET, can enable the surface of a PET film to show obvious holes, and can be used as a candidate enzyme for marine in-situ degradation of PET plastics. The PET hydrolase disclosed by the invention is high in activity, low-temperature-resistant and high-temperature-resistant, is a seawater environment-like PET plastic, and can provide theoretical guidance and technical support for degradation of marine micro-plastics in the future.

The invention provides a bio-based filtrate reducer as well as a preparation method and application thereof. The environment-friendly zwitterionic bio-based filtrate reducer is obtained by taking waste small dragon shrimp and crab shells as raw materials, treating the raw materials by adopting an acid-alkali method to obtain glycosaminoglycan, performing carboxylation modification on the glycosaminoglycan through monochloroacetic acid, and copolymerizing the glycosaminoglycan with a propenyl monomer, a quaternary ammonium salt monomer and 2-acrylamide-2-methylpropanesulfonic acid. The bio-toxicity EC50 value of the bio-based filtrate reducer provided by the invention is greater than 100000 mg / L, the biodegradability BOD / CODcr value is greater than 0.05, and the bio-based filtrate reducer belongs to a non-toxic and easily degradable level; the prepared filtrate reducer has good filtrate reduction property and temperature resistance at normal temperature and 100-200 DEG C, and still has excellent temperature resistance and salt resistance after being added into brine base slurry or saturated brine; the prepared filtrate reducer is wide in raw material source and low in price, can be applied to geological drilling operation to maintain the stability of a well wall, and provides guarantee for smooth proceeding of the geological drilling operation.

This invention belongs to the field of molecular biology and discloses a SNP marker associated with salt tolerance in largemouth bass and its application. This invention obtains an SNP molecular marker related to the strength of salt tolerance in largemouth bass by performing correlation analysis on the salt tolerance of extreme and sensitive individuals. The SNP molecular marker, as shown in SEQ ID NO:1, is located at position 501 and has three genotypes: TT, TG, and GG. The GG genotype largemouth bass exhibits a significantly longer survival time under salinity stress than the TG and TT genotypes. By detecting the genotype of this SNP molecular marker, largemouth bass parents with strong salt tolerance can be accurately selected, which can be effectively used for marker-assisted breeding of largemouth bass.

This invention relates to genetically improved Eastern oyster (Crassostrea virginica) lines developed by the Morgan State University Patuxent Environmental and Aquatic Research Laboratory. Derived from Maryland wild populations, these diploid, triploid, and tetraploid lines exhibit enhanced tolerance to low salinity, rapid growth, and disease resistance. Genetic improvement was achieved through multiple approaches including phenotype-based, genomic, and marker-assisted selection. Several diploid low-salinity-tolerant lines (LS2019, LS2025, LS H-GEBV) and a fast-growing line (FG H-GEBV) have been produced and are undergoing field evaluation, while MSX-resistant lines are under development through field challenge testing. Multi-trait genomic selection enables integration of growth, salinity tolerance, and disease resistance into single lines based on trait correlations and production needs. Triploid lines (3nD-20, 3nE-20, 3 nF-20, 3nW-25) generated by chemical induction are used to create tetraploids, ultimately producing superior commercial triploid seed. These distinct oyster lines improve aquaculture productivity and restoration success in low-salinity Chesapeake Bay environments.