Preparation method and application of immobilized agent bag of efficient denitrifier
A technology of denitrification bacteria and bacterial agents, applied in the field of water pollution control, can solve the problems of large amount of bacterial agents used, low utilization efficiency, and inability to recycle, and achieve the effects of efficient removal, high treatment costs, and increased residence time
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Embodiment 1
[0027] Sampling the sediments of a mariculture area, the concentration of ammonia nitrogen in the sediments was detected to be 238mg / L, and the concentration of nitrate was 254mg / L. The immobilized bacterial agent package was used to test the ammonia nitrogen and Nitrate nitrogen inhibition experiment, including the following steps:
[0028] First, screen out efficient heterotrophic nitrifying and aerobic denitrifying bacteria: Weigh 10g of seabed sediment with a dry weight, add it to a 250mL Erlenmeyer flask filled with 100mL enrichment medium, and set the temperature at 150r / min at 28°C After several days of shaking culture, the supernatant was taken into the separation medium, and shaking culture was carried out under the above conditions for several days. Then, take 10 mL of the supernatant to a fresh separation medium, shake and culture at 150r / min, 28°C for several days, repeat the above steps for 3 times to increase the concentration of heterotrophic nitrifying and aero...
Embodiment 2
[0035] Sampling the sediments of a mariculture area, the concentration of ammonia nitrogen in the sediments was detected to be 46mg / L, and the concentration of nitrate was 39mg / L. Nitrate nitrogen removal experiment, including the following steps:
[0036] First, screen out efficient heterotrophic nitrifying and aerobic denitrifying bacteria: weigh 20g of seabed sediment with a dry weight, add it to a 250mL Erlenmeyer flask filled with 100mL enrichment medium, and set the temperature at 200r / min, 30°C After several days of shaking culture, the supernatant was taken into the separation medium, and shaking culture was carried out under the above conditions for several days. Then, take 10 mL of the supernatant to a fresh separation medium, shake and culture it at 200r / min, 30°C for several days, repeat the above steps for 3 times to increase the concentration of heterotrophic nitrifying and aerobic denitrifying bacteria, and finally Obtain heterotrophic nitrification aerobic den...
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