77 results about "Aerobic denitrification" patented technology

The invention discloses a nitrifying bacterium agent suitable for aquarium, fishpond and other aquatic industries, the strain of the nitrifying bacterium agent is Glutamicacter nicotianae TJ-X, the nitrifying bacterium agent is preserved in Guangdong Microbial Culture Collection Center on February 12, 2025, and the preservation number is GDMCC NO.65877. The invention further discloses a preparation method of the nitrifying bacterium agent. The nitrifying bacteria TJ-X strain suitable for the aquarium industry can perform heterotrophic nitrification and aerobic denitrification under the condition that the salt content is 5wt%, under the condition of laboratory pure culture, within 8 hours, the removal rate of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen is 99% or above, the COD degradation rate is 81%, the nitrifying bacteria TJ-X strain is suitable for the environment where organic matter, ammonia nitrogen, nitrite and nitrate coexist, and the nitrifying bacteria TJ-X strain can be applied to the aquarium industry. The microbial agent has the functions of degrading organic matters such as excrement of ornamental fish and fish food, converting organic nitrogen into inorganic nitrogen, and simultaneously degrading inorganic nitrogen such as ammonia nitrogen, nitrite nitrogen and nitrate nitrogen into nitrogen. And the strain is high in biological safety, high in environmental adaptability and high in growth density, can be applied to water industries with high organic matter content and inorganic nitrogen, such as the aquarium industry and aquaculture, and has a wide application prospect.

The invention belongs to the field of microorganisms, and particularly relates to a high-efficiency denitrification heterotrophic nitrification-aerobic denitrification klebsiella and a culture method and application thereof.A biological material of the high-efficiency denitrification heterotrophic nitrification-aerobic denitrification klebsiella is named as Klebsiella oxytoca CT4 and is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on July 21, 2025, the preservation number of the Klebsiella oxytoca CT4 is CGMCC NO.9687, and the preservation number of the Klebsiella oxytoca CT4 is CGMCC NO.987. The preservation address is Institute of Microbiology, Guangdong Academy of Sciences, on the fifth floor of building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou, Guangdong Province; the preservation number of the strain is GDMCCNO: 66723; according to the strain, the ammonia nitrogen removal rate can reach 98.7% through nitrification-denitrification, the nitrite accumulation amount in the whole process is lower than 0.5 mg / L, and accumulation of toxic intermediate products is avoided. The strain has wide environmental adaptability, can keep efficient denitrification activity under the conditions that the pH is 5.0-10.0 and the C / N ratio is 5-30, can tolerate fluctuation of wastewater quality, does not need to repeatedly adjust the pH value when being used for treating aquaculture wastewater with a high C / N ratio (greater than 20), and can still stabilize the total nitrogen removal rate to be 80% or above, so that the addition cost in a conventional process is remarkably reduced.

The utility model belongs to the technical field of biological denitrification of industrial aquaculture water, and particularly relates to a denitrification device suitable for an industrial aquaculture system. Comprising an aerobic nitrification zone, a carbon source zone, an aerobic denitrification zone and a suspended particle removal zone which are communicated in sequence, a filter material is arranged in the aerobic nitrification zone, and the aerobic nitrification zone is used for carrying out biological oxidation on aquaculture water to generate nitrate; the upper part of the carbon source region is filled with carbon sources; the aerobic denitrification area is used for reducing nitrate in the water body into nitrogen; the suspended particle removal area is used for removing suspended particles in the water body. A water inlet pipe is arranged at the bottom of one side wall of the aerobic nitrification area, an aerobic nitrification area overflow port is formed in the upper part of the other side wall of the aerobic nitrification area, and the internal space of the aerobic nitrification area is distributed in a shape; a water outlet pipe is arranged at the bottom of the side wall of the suspended particle removal area. According to the device, aerobic nitrification, aerobic denitrification and suspended particulate matter removal are organically integrated, intensification and modularization are realized, the device is simple in structure and convenient to operate, and the culture denitrification effect is effectively improved.

The invention relates to the technical field of environmental microbial resource development and sewage denitrification. The invention discloses an efficient denitrification strain Paracoccus sp.N21 with a flocculation characteristic, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34710. The invention further discloses a preparation method of the efficient denitrification strain Paracoccus sp.N21. The strain obtained by the invention can completely remove ammonia nitrogen within 12 hours and thoroughly remove nitrate nitrogen within 48 hours, the removal rate reaches 100%, and ammonia nitrogen is preferentially degraded in a mixed nitrogen source. The method has the unique advantages that the flocculation rate in the nitrification process and the flocculation rate in the denitrification process exceed 93.9%, and the usage amount of a flocculating agent can be greatly reduced. When actual kitchen wastewater is treated, the ammonia nitrogen removal rate reaches 100%, and the removal rate reaches up to 7.51 mg / (L.h). The application of the strain can effectively reduce the volume of a reactor, reduce energy consumption and operation cost, provides a new solution for simplifying a biological denitrification process, is particularly suitable for treating high-ammonia-nitrogen kitchen wastewater, and has remarkable environmental and economic benefits.

The invention discloses Pseudomonas tai H1R1 and application thereof in treatment of mariculture tail water, the Pseudomonas tai H1R1 has excellent high salt resistance and aerobic denitrification function, can directly convert ammonia nitrogen and nitrate into nitrogen and efficiently remove nitrogen, the nitrogen removal rate can reach 80% or above, nitrogen load in water is reduced, the nitrogen removal efficiency is improved, and the application of the Pseudomonas tai H1R1 in treatment of mariculture tail water is improved. The problem of water eutrophication caused by nitrogen can be prevented; the system can survive and breed under various severe environment conditions and treat mariculture tail water. The pseudomonas tai H1R1 disclosed by the invention can utilize organic matters in mariculture tail water as a carbon source, so that the growth speed and the growth amount of bacteria are increased, and organic wastes in a culture process are reduced, so that the strain H1R1 can promote self growth while treating the mariculture tail water; and double benefits of environmental governance and resource cyclic utilization are realized.

A low-temperature-resistant heterotrophic nitrification and aerobic denitrification bacteria is used for wastewater denitrification method, relates to a kind of biological bacteria for wastewater denitrification method, the bacteria is Pseudomonas umsongensis BK17, belongs to Pseudomonas, is preserved in China typical culture preservation center, and the preservation number is CCTCC M 20242924, and the preservation date is December 27, 2024.The strain is a heterotrophic nitrification-aerobic denitrification bacteria, can efficiently remove inorganic nitrogen pollution in water body, and still has good denitrification performance under low temperature stress, and has strong low temperature tolerance.The strain can efficiently treat nitrogen-containing wastewater at 10 DEG C, and the removal efficiency can reach more than 99%.In the inorganic nitrogen pollution treatment and application of various water bodies such as domestic sewage in cold regions of China, it has great potential.

The invention relates to the technical field of soil conditioners and applications thereof, and discloses a soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum and an application thereof in ploughing ability improvement, the soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum comprises an organic pollutant degradation functional flora, a soil carbon sequestration functional flora and a small molecular organic matter production functional flora; the organic pollutant degrading functional flora comprises efficient pollutant degrading bacteria; the soil carbon sequestration functional flora comprises ammonia oxidizing bacteria, ammonia oxidizing archaea, complete ammonia oxidizing bacteria, sulfate reducing bacteria, iron reducing bacteria, rhizobium and photosynthetic flora; the micromolecular organic matter production functional flora comprises heterotrophic nitrification-aerobic denitrification bacteria, zoogloea, phosphorus-accumulating bacteria and photosynthetic bacteria. According to the scheme, multiple florae are specifically arranged in the synergistic agent, and through the synergistic interaction effect of the florae, the effects of the synergistic agent in multiple fields of soil carbon sequestration, pollution reduction and yield increase are effectively improved.

The application belongs to the field of environmental governance, and provides a water sodium manganese ore-oxygen releasing agent composite type sediment repair material and a preparation and application method thereof, which comprises modified oxygen releasing agent, water sodium manganese ore, microbial flora and embedding matrix, the embedding matrix comprises calcium alginate, polyvinyl alcohol and water cross-linked formed hydrogel, wherein the microbial flora comprises domesticated aerobic denitrification and phosphorus removal mixed flora.The water sodium manganese ore-oxygen releasing agent composite type sediment repair material utilizes the photoelectric catalytic activity of water sodium manganese ore and the oxygen releasing ability of oxygen releasing compound, improves the oxidation-reduction potential of sediment, enhances the sustained degradation ability of organic pollutants, cooperatively removes nitrogen pollutants in water environment, enhances the in-situ repair effect of sediment, is applied to sediment repair, and is more efficient, environment-friendly and has long-term effect.

The invention relates to the technical field of biological sewage treatment, in particular to a method for realizing heterotrophic nitrification-aerobic denitrification nitrogen removal of wastewater with a low carbon nitrogen ratio. According to the method for realizing heterotrophic nitrification-aerobic denitrification nitrogen removal of the wastewater with the low carbon-nitrogen ratio, an exogenous magnetic field is introduced into a slow-release carbon source system, so that the central magnetic field intensity of an aeration tank is 10-40mT, and the metabolic activity of cellulose degrading bacteria in the system is enhanced and the degradation process of cellulose is promoted by utilizing a magnetic biological effect excited by the magnetic field; the carbon source release efficiency is improved, and the problem of limited carbon release amount of the slow-release carbon source is solved. The enhanced carbon release process effectively increases the carbon nitrogen ratio, provides sufficient organic carbon sources and electron donors for heterotrophic nitrification-aerobic denitrification bacteria, makes the heterotrophic nitrification-aerobic denitrification bacteria have advantages in competition with autotrophic bacteria, further enhances the dominant position of heterotrophic nitrification-aerobic denitrification in the denitrification process without changing other operating parameters, realizes efficient denitrification, and improves the denitrification efficiency. Cost is low, operation is simple, and effect is remarkable.

The invention provides a pseudomonas for denitrification nitrogen removal, a microbial inoculum, an iron composite material combined preparation as well as a method and application thereof, and relates to a biological denitrification technology. The invention provides pseudomonas sp. AH40, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20221280, and the pseudomonas is preserved in the China Center for Type Culture Collection on August 15, 2022 and has the aerobic denitrification capability; the invention also provides an iron composite material, the iron composite material can be combined with pseudomonas AH40 to prepare a combined preparation, and the combined preparation can further improve the nitrogen removal rate in the culture medium.

The present application belongs to the technical field of excrement treatment, and particularly relates to an integrated rural toilet excrement deep separation treatment equipment, aiming at the problems of poor operation stability, and unsatisfactory removal rate of total phosphorus and ammonia nitrogen pollutants of the existing equipment, the present application proposes the following scheme, including a solid-liquid separation module, an anaerobic treatment tank, an aerobic treatment tank, and a liquid phase flocculation unit, the present application separates the solid phase and the liquid phase of the excrement through the solid-liquid separation module, after the liquid phase is flocculated by the liquid phase flocculation unit, the liquid phase and the solid phase are put into the corresponding fermentation zones of the anaerobic treatment tank for anaerobic fermentation treatment, the liquid phase is transported to the aerobic treatment tank for further aerobic treatment, realizing integrated treatment of solid-liquid separation, anaerobic biogas production and aerobic denitrification of the rural toilet excrement, and the solid phase and the liquid phase after the integrated treatment can be further utilized, which is extremely suitable for use in rural areas.

The application relates to the field of microbiology and presents a salt-tolerant heterotrophic nitrification aerobic denitrifying and phosphorus removal bacterial strain and its application. The strain is Pseudomonas mendocina A4, deposited on Nov. 4, 2022, at the Guangdong Microbial Culture Collection Center, located at Building 59, 5th Floor, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, with the deposit number GDMCC No: 62944. The Pseudomonas mendocina A4 strain provided by the present application possesses both heterotrophic nitrification and aerobic denitrification functions and can be applied in the field of wastewater treatment. It has a strong tolerance to high concentrations of organic carbon while utilizing various organic carbon sources in wastewater and exhibits excellent organic carbon removal capabilities in water. This strain is particularly suitable for treating nitrogen-containing wastewater with a high C / N ratio.

The invention provides a strain for sewage treatment under low-temperature and low-carbon-nitrogen-ratio conditions and application of the strain, and belongs to the technical field of microbial fermentation treatment. The bacterial strain is Pseudomonas sp. HR2, the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No.32795, and the preservation date of the bacterial strain is November 25, 2024. The pseudomonas HR2 has the biggest characteristic of integrating two major functions of heterotrophic nitrification and aerobic denitrification. The ammonia nitrogen and nitrate nitrogen in sewage can be efficiently and synchronously removed under the conditions of low temperature of 2-10 DEG C and low carbon nitrogen ratio of C / N = 3-8. Therefore, the method effectively solves the problem of efficiency attenuation of a traditional denitrification technology in a low-temperature and low-carbon-source environment, and has the remarkable technical effects of process simplification, energy conservation, consumption reduction and cost control.

The invention discloses a bacterial-algae symbiotic integrated culture tail water treatment system and method. The culture tail water treatment system comprises a regulating reservoir, a submersible sewage pump, a multi-stage coupling biochemical treatment unit and a shellfish purification unit, the submersible sewage pump is arranged at an outlet of the adjusting tank; the multi-stage coupling biochemical treatment unit comprises an aeration biochemical pool, a biological filter bed and an aerobic denitrification microalgae biological membrane unit which are sequentially connected in series; the biological filter bed takes a rice husk layer and a pelelith layer as biological carriers; the aerobic denitrification microalgae biological membrane unit comprises microalgae and functional bacteria, the functional bacteria are bacilli weldmanni and escherichia marmoreus, the microalgae fix CO2 and release oxygen through photosynthesis, and the functional bacteria degrade nitrides through heterotrophic nitrification-aerobic denitrification; the shellfish purification unit is arranged at the water outlet end of the multi-stage coupling biochemical treatment unit and is used for filtering residual microalgae and suspended particulate matters in the water body. The invention further provides a tail water treatment method, nitrogen and phosphorus are efficiently removed, energy consumption is reduced, secondary pollution is avoided, and resource utilization can be achieved.

This invention discloses a membrane bioreactor (MBR) treatment method and a field return system for wastewater from sheep slaughterhouses. After three-stage pretreatment, the wastewater undergoes two-stage anoxic-aerobic denitrification, with segmented influent and two-stage internal recirculation. A short-range nitrification window is created in the first-stage aerobic zone with low dissolved oxygen through intermittent aeration, while stable nitrification occurs in the second-stage aerobic zone. The biochemically treated effluent enters a PVDF submerged ultrafiltration (MBR), where the mixed liquor suspended solids concentration and flux are controlled. Backwashing / relaxation and chemical cleaning sequences are triggered by the increase in transmembrane pressure difference per unit time, enhancing shock and fouling resistance. MBR permeate is fed into a field return tank and connected to the field return system. Precise water and fertilizer return and salt control are achieved through rapid nitrogen and phosphorus measurement, application decision-making, and stratified drip irrigation valve control. This scheme can achieve effluent ammonia nitrogen ≤3 mg / L, high total nitrogen removal, and reduced membrane fouling and nitrogen loss.

The invention provides a biological Fenton coupled heterotrophic nitrification-aerobic denitrifying bacterium NMP-containing wastewater treatment method, which comprises the following steps: S1, putting immobilized glucose oxidase and glucose into NMP-containing wastewater to react, and recovering the immobilized glucose oxidase after the reaction is finished to obtain H2O2-containing first wastewater; s2, adding Fe < 2 + > into the first wastewater for reaction to obtain second wastewater; and S3, putting the heterotrophic nitrification-aerobic denitrification bacteria into the second wastewater for reaction.

The invention belongs to the field of environmental microbiological technology and wastewater biological treatment, and particularly discloses a synchronous nitrification and denitrification acid-eating bacterium and application thereof in wastewater treatment. The strain is Acidovorax sp. LM14, and is preserved in China Center for Type Culture Collection, called CCTCC for short, the preservation address is Wuhan University Life Science College, Wuchang District, Wuhan City, Hubei Province, the preservation date is November 10, 2025, and the preservation number is CCTCC M 20252521. The strain has a heterotrophic nitrification-aerobic denitrification capability, can synchronously and efficiently remove ammonia nitrogen, nitrite nitrogen and nitrate nitrogen under an aerobic condition, and still can efficiently remove wastewater with the ammonia nitrogen concentration of 500mg / L. Meanwhile, the strain can effectively degrade aromatic pollutants such as phenol, and the degradation rate exceeds 90% when the concentration of phenol is as high as 500mg / L. In addition, the LM14 still keeps high denitrification activity under the condition of low carbon nitrogen ratio, and has a wide application range to pH and temperature.

The invention relates to the field of environmental microorganisms, and discloses a heterotrophic nitrification aerobic denitrification nitrogen and phosphorus removal strain with a salt-tolerant characteristic, a microbial inoculum and application, the strain is Acinetobacter haemolticus GE10 which is preserved in the Guangdong Microbiological Culture Collection Center on May 12, 2025, the preservation address is the fifth floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City, Guangdong Province, and the preservation number is CGMCC NO.987. The invention further discloses a preparation method of the heterotrophic nitrification aerobic denitrification nitrogen and phosphorus removal strain with the salt-tolerant characteristic. The preservation number of the strain is GDMCC NO. 66303. The acinetobacter hemolyticus strain GE10 domesticated by the invention has the functions of salt resistance, heterotrophic nitrification and aerobic denitrification at the same time, has better water organic carbon removal capacity, and is particularly suitable for treatment of high-salinity nitrogen-containing sewage.

A strain of antibiotic-resistant marine heterotrophic ammonia assimilation-aerobic denitrifying bacteria and its application, belongs to the field of water treatment of aquaculture. The seawater antibiotic-resistant high-efficiency nitrogen removal Marinobacter nitratireducens LAN01 can use polyhydroxyalkanoate as the sole carbon source, and can realize heterotrophic ammonia assimilation and aerobic denitrification function under the stress of different concentrations of sulfamethoxazole antibiotics, and simultaneously transform phosphate. The strain has multiple resistances such as high salinity, high alkalinity and antibiotic, and can be used for seawater aquaculture effluent treatment, seawater recirculating aquaculture water treatment, high-salinity wastewater treatment and saline-alkali soil bioremediation, etc. To realize the simultaneous recovery of nitrogen, phosphorus and other nutrients in the seawater aquaculture environment.

This invention relates to the fields of environmental microbiology and wastewater treatment technology, specifically to a highly salt-tolerant Acinetobacter baumannii strain for heterotrophic nitrification and aerobic denitrification of wastewater, a microbial agent, and its application. The strain is Acinetobacter baumannii. Acinetobacter baumannii M4, deposited on May 12, 2025, at the Guangdong Provincial Microbial Culture Collection Center, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, with accession number GDMCC No: 66302. This invention utilizes the *Acinetobacter baumannii* strain M4, domesticated using a multi-round salt-beating method. It can grow in high-salt-alkali environments and exhibits both heterotrophic nitrification and aerobic denitrification capabilities. It can be widely applied to the biological denitrification and phosphorus removal treatment of high-salt-alkali nitrogen and phosphorus wastewater, including wastewater from aquaculture, pickling, and industrial processes.

The invention discloses a pseudomonas stutzeri SND-1 strain as well as a culture method and application thereof. According to the invention, a broad-salt synchronous nitrification and denitrification functional strain SND-1 capable of efficiently degrading nitrogen concentration in an aquaculture water body is separated from a prawn aquaculture pond, and the strain is identified as Stutzeria stutzeri according to colonial morphology and 16S rDNA gene sequencing analysis and phylogenetic analysis, the pseudomonas stutzeri SND-1 strain is preserved in the China Center for Type Culture Collection (the preservation number is CCTCC NO: M 20242216) on October 15, 2024, and the pseudomonas stutzeri SND-1 strain is preserved in the China Center for Type Culture Collection (CCTCC). The pseudomonas stutzeri SND-1 strain belongs to heterotrophic ammonia oxidizing bacteria, has a heterotrophic nitrification-aerobic denitrification function, can efficiently degrade ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and total nitrogen in fresh water, seawater and aerobic environments, has no toxic effect on aquatic animals, and can be applied to ecological restoration of fresh water or seawater water environments.

The embodiment of the invention discloses aspergillus niger NO1 and application thereof. The Aspergillus niger NO1 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center on November 21, 2025, the preservation address is the 5th floor, No.59 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 67338. According to the invention, the bottom mud and high-enrichment culture sewage of sea farms in Zhangzhou Dragon Sea as well as Zhangzhou Dragon Sea are used as samples, and are subjected to primary screening and secondary screening culture to obtain a strain with excellent growth performance and good utilization performance on sodium nitrite. Through identification, the strain is aspergillus niger NO1, has an efficient aerobic denitrification function, shows excellent degradation efficiency on nitrite in aquaculture water, is good in safety, does not generate adverse effects on aquaculture organisms and water environment, and has a wide practical application prospect in the field of aquaculture water quality purification.

The present application belongs to the technical field of microbial denitrification, and particularly relates to a method for improving the denitrification performance of biological bacteria. The biological bacteria are cultured in an environment containing carbon quantum dots, inorganic salts, a carbon source and a nitrogen source at 28 DEG C to 32 DEG C for 10 h to 16 h. The addition of 4 mu g / L to 6 mu g / L of carbon quantum dots can significantly improve the activity of heterotrophic ammonia assimilation and aerobic denitrification bacteria, increase the heterotrophic ammonia assimilation efficiency by 27.6%, increase the aerobic denitrification efficiency by 23.08%, and improve the denitrification efficiency.

The application relates to the field of environmental microorganism technology and discloses a salt-tolerant aerobic denitrification bacterium and a method for simultaneously realizing denitrification and carbonate precipitation and carbon fixation. Zobellella denitrificans A36, which is preserved in the Guangdong Microbial Culture Collection Center and has a preservation number of GDMCC No: 66789 and a preservation date of August 7, 2025. The Zobellella denitrificans A36 has complete denitrification key functional genes and carbonic anhydrase related gene clusters; can induce the formation of carbonate precipitation in a salt-containing heterotrophic denitrification system, fixes CO2 generated by the system into inorganic carbonate, and effectively reduces CO2 emission. Under preferred conditions, the removal rate of nitrate nitrogen can reach 93.8%, and stable carbonate carbon fixation effect is realized.

The invention discloses a pseudomonas strain, the pseudomonas strain is a heterotrophic nitrification and aerobic denitrification bacterium DHZK147, the pseudomonas strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO: 34640. The strain obtained through screening and separation has the efficient denitrification capacity and can achieve synchronous nitrification and denitrification. Compared with the traditional anaerobic denitrifying bacteria, the method has the advantages that the treatment time and cost of the process are saved in the biological denitrification process, and the effective removal of ammonia nitrogen is realized.

The application discloses a heterotrophic nitrification-aerobic denitrification bacterium and application thereof in sewage denitrification treatment, and belongs to the technical field of microorganisms. Enterobacter asburiae The heterotrophic nitrification-aerobic denitrification bacterium is Enterobacter asburiae WZCH (WZCH), which was preserved in the China Center for Type Culture Collection on June 16, 2025, and the preservation number is CCTCC NO: M 2025272. The strain has high reduction capacity, can convert ammonia nitrogen, nitrate nitrogen and the like in sewage into gaseous nitrogen, has no nitro nitrogen accumulation, is green and environment-friendly, and has high practical application value.

The invention discloses a method for screening a high-performance mixed carbon source for sewage treatment, and belongs to the technical field of sewage treatment, and the method comprises the following steps: taking original sludge, and carrying out aerobic denitrification domestication; adding nitrate and carbon sources into the domesticated sludge to determine specific denitrification rates and denitrification COD utilization rates under different carbon sources; determining aerobic denitrification performance scores of different carbon source combinations based on the specific denitrification rate, the denitrification COD utilization rate and the unit COD price; fitting models by taking aerobic denitrification performance scores of different carbon source combinations as response values, and screening out an optimal fitting model; and calculating an optimal solution based on the optimal fitting model so as to determine the carbon source combination. The invention solves the problem that a single carbon source is difficult to optimize performance indexes such as denitrification rate, denitrification chemical oxygen demand utilization rate, cost and the like of the aerobic denitrification process for sewage treatment at the same time.

The invention relates to a bacillus altitudinis ZT61 (bacillus altitudinis ZT61) with an aerobic denitrifying and phosphorus accumulating function and application of the bacillus altitudinis ZT61 in treatment of wastewater with high nitrogen content and phosphorus content. The strain has the capability of synchronously removing ammonia nitrogen and phosphorus, can finally convert nitrogen pollutants into gas products, is suitable for treating high-nitrogen-content organic wastewater, has obvious environmental adaptability and interspecific competitive advantages, can efficiently grow at 15-35 DEG C, and can remove nitrogen and phosphorus. Compared with a traditional denitrification process, the strain is high in denitrification efficiency, low in energy consumption, simple to operate and high in stability, and application of the strain provides a certain basis for sewage treatment.

This invention belongs to the field of biotechnology and discloses a heterotrophic nitrifying-aerobic denitrifying Acinetobacter pittii AP-3 and its applications. The Acinetobacter pittii AP-3 has the accession number GDMCC No: 68038, taxonomical name Acinetobacter pittii, accession date April 2, 2026, and is deposited at the Guangdong Provincial Microbial Culture Collection Center, located at 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou. The Acinetobacter pittii AP-3 provided by this invention exhibits good heterotrophic nitrification-aerobic denitrification performance. Under conditions of 30℃, C / N ratio of 25, sodium succinate as the carbon source, 5% inoculum size, and pH 7, when treated with a heterotrophic nitrification-aerobic denitrification medium (SNDM), NO2... ‑ The removal rate of NO3- concentration reached 98.52% after 24 hours. ‑ -N concentration removal rate reaches over 95%, NH4 + The nitrogen removal rate reached 100%. Moreover, the AP-3 strain has high tolerance to pH, and exhibits good denitrification effect in the pH range of 5 to 9.

The application discloses a kind of based on magnetic field-modified polyurethane composite combined strengthening denitrification device and method, including water inlet area, gravel packing area, aeration zone, polyurethane packing tray area, sludge discharge area, water outlet area and PLC controller, nZVI is configured with gravel and strong magnet in gravel packing area @SiO2, under the action of strong magnetic field, anaerobic denitrification is carried out to slightly polluted water, and part of organic matter and nitrate is degraded;And in water inlet area, aeration tray is configured to backflush;In polyurethane packing tray area, modified polyurethane sponge packing tray is configured, and aerobic denitrification and microbial denitrification are carried out to aeration oxygenated water body, and sludge is generated and precipitated.The application solves the problems of unstable release speed of inorganic electron donor, poor synergistic effect with microorganisms and other problems, avoids the loss of bacterial flora, slows down the biofilm formation time in the later stage, promotes the biological activity of microorganisms, more effectively promotes the process of enhanced denitrification of water body, and realizes efficient denitrification of slightly polluted water body.