208 results about "Aerobic denitrification" patented technology

The invention relates to the technical field of microorganisms, and provides a low-temperature-resistant heterotrophic nitrification-aerobic denitrification pseudomonas and application thereof, the strain is pseudomonas (Pseudomonas sp.) SW1, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20221797. The low-temperature-resistant heterotrophic nitrification-aerobic denitrification bacterium Pseudomonas sp. SW1 has the characteristic of low temperature resistance, can well grow at 4-10 DEG C, and has relatively strong heterotrophic nitrification-aerobic denitrification nitrogen removal capability, and the enrichment culture of the strain is carried out in a relatively low nitrogen source, and the condition is extremely consistent with the application scene of the strain in domestic sewage.

The invention discloses low-temperature-resistant and high-salt-resistant aerobic pseudomonas kirschner kirschner K2 as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out low-temperature and high-salt enrichment culture; screening and domesticating, and monitoring the concentration of ammonia nitrogen; and after 3-5 times of enrichment culture, diluting the enrichment culture, further culturing until a macroscopic bacterial colony is obtained, and then picking and carrying out plate streaking to separate a pure strain. The invention also relates to application of the strain in preparation or prevention of nitrification and denitrification nitrogen removal drugs in treatment of high-salt nitrogen-containing wastewater. The strain is low-temperature-resistant and high-salt-resistant heterotrophic-aerobic denitrifying bacteria, has the characteristic of low temperature resistance, grows well at low temperature and high salt salinity, has higher heterotrophic nitrification-aerobic denitrification nitrogen removal capacity, is suitable for nitrogen removal treatment of high-salt wastewater such as food processing wastewater and landfill leachate, and has wide application prospects. The superiority is particularly obvious under a low-temperature condition.

The invention belongs to the technical field of microorganisms, and discloses a low-temperature-resistant aerobic denitrifying bacterium and application thereof. The low-temperature-resistant aerobic denitrifying bacterium is Enterobacter ludwigii CW-01, and the preservation number of the low-temperature-resistant aerobic denitrifying bacterium is CGMCC (China General Microbiological Culture Collection Center) No.27126. The low-temperature-resistant aerobic denitrifying bacterium can be used for preparing the The enterobacter lewis CW-01 strain disclosed by the invention can efficiently realize aerobic denitrification degradation of ammonia nitrogen, nitrate and nitrite within the temperature range of 4-35 DEG C, and is especially suitable for wastewater treatment in which the ammonia nitrogen, nitrate and nitrite are difficult to remove due to low temperature; the strain also has good salt resistance, and provides a good microbial material for biological nitrogen removal treatment of low-temperature, high-salt and high-ammonia nitrogen polluted water. The nitrogen is efficiently removed, meanwhile, the use amount of a carbon source is remarkably reduced, the enterprise cost is greatly reduced, and the method has a good application prospect in the field of sewage treatment.

The invention provides an aerobic denitrifying bacterium with zoogloea character and application thereof, and belongs to the technical field of wastewater treatment, the aerobic denitrifying bacterium is specifically acinetobacter baumannii N-1 (Acinetobacter sp.N-1), the acinetobacter baumannii is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20242568. The acinetobacter baumannii N-1 provided by the invention not only can perform wastewater denitrification through aerobic denitrification and has efficient denitrification capacity, but also can realize separation of sludge and purified water by forming zoogloea, so that the acinetobacter baumannii N-1 has good application potential in wastewater treatment by utilizing an activated sludge process.

The invention relates to the technical field of microorganisms, in particular to a seawater efficient heterotrophic nitrification and aerobic denitrification strain and application thereof. The strain is Marinobacter sp. TF26-2, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 27, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.30778. The invention further discloses a preparation method of the strain. The marinobacter TF26-2 provided by the invention has the functions of heterotrophic nitrification and aerobic denitrification at the same time, and can be used in the field of sewage treatment. The strain shows a good denitrification effect under different environmental conditions, is safe to aquatic organisms, and is especially suitable for being used as probiotics or an environmental modifier in the fields of mariculture and the like.

The invention discloses a nitrifying bacterium agent suitable for aquarium, fishpond and other aquatic industries, the strain of the nitrifying bacterium agent is Glutamicacter nicotianae TJ-X, the nitrifying bacterium agent is preserved in Guangdong Microbial Culture Collection Center on February 12, 2025, and the preservation number is GDMCC NO.65877. The invention further discloses a preparation method of the nitrifying bacterium agent. The nitrifying bacteria TJ-X strain suitable for the aquarium industry can perform heterotrophic nitrification and aerobic denitrification under the condition that the salt content is 5wt%, under the condition of laboratory pure culture, within 8 hours, the removal rate of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen is 99% or above, the COD degradation rate is 81%, the nitrifying bacteria TJ-X strain is suitable for the environment where organic matter, ammonia nitrogen, nitrite and nitrate coexist, and the nitrifying bacteria TJ-X strain can be applied to the aquarium industry. The microbial agent has the functions of degrading organic matters such as excrement of ornamental fish and fish food, converting organic nitrogen into inorganic nitrogen, and simultaneously degrading inorganic nitrogen such as ammonia nitrogen, nitrite nitrogen and nitrate nitrogen into nitrogen. And the strain is high in biological safety, high in environmental adaptability and high in growth density, can be applied to water industries with high organic matter content and inorganic nitrogen, such as the aquarium industry and aquaculture, and has a wide application prospect.

The invention discloses a heterotrophic nitrification and aerobic denitrification halomonas sp. ZC-1 with low temperature resistance and high salt, and the preservation number of the heterotrophic nitrification and aerobic denitrification halomonas sp. ZC-1 is CCTCC (China Center for Type Culture Collection) M 20241695. The strain ZC-1 screened by the invention can simultaneously tolerate the influence of low temperature and high salt, and still can efficiently remove ammonia nitrogen under double stress conditions, especially under the environment of the temperature of 10 DEG C and the salinity of 10% w / v, the ammonia nitrogen removal rate reaches 94% or above, the TN removal rate reaches 93% or above, and salt-containing wastewater in northern China in winter can be treated in a targeted manner.

The invention discloses a heterotrophic nitrification-aerobic denitrification bacterium resistant to high ammonia nitrogen and high organic matters and application of the heterotrophic nitrification-aerobic denitrification bacterium in landfill leachate treatment. The heterotrophic nitrification-aerobic denitrification bacterium provided by the invention is Paracoccus pantotrophus HNAD-9, the strain is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms on August 22, 2024, and the preservation number of the strain is CGMCC No.31698. The invention further discloses a preparation method of the heterotrophic nitrification-aerobic denitrification bacterium. According to the method, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen can be removed in the same heterotrophic and aerobic system at the same time, accumulation of nitrate nitrogen and nitrite nitrogen is avoided in the ammonia nitrogen removal process, and the method has important application prospects in the field of high-ammonia-nitrogen and high-organic-matter-concentration landfill leachate treatment.

The invention relates to the field of sewage treatment, in particular to a treatment method and system for a sludge hydrothermal carbonization solution. The treatment method provided by the invention comprises the following steps: adjusting the pH value of the sludge hydrothermal carbonization solution to 5-6 to obtain an initially adjusted carbonization solution; carrying out hydrolytic acidification reaction on the primarily adjusted carbonized solution to obtain a hydrolytic acidification carbonized solution; carrying out methanogenesis reaction on the hydrolytic acidification carbonization solution to obtain an anaerobic digestion carbonization solution; performing multi-stage anaerobic-aerobic denitrification treatment on the anaerobic digestion carbonization solution to obtain a denitrified carbonization solution; dephosphorizing the denitrified carbonized solution to obtain a dephosphorized carbonized solution; and carrying out resin adsorption on the dephosphorized carbonized liquid, and then discharging the dephosphorized carbonized liquid. The treatment method provided by the invention can effectively remove organic matters and nitrogen and phosphorus compounds contained in the sludge hydrothermal carbonization liquid. The concentrations of various pollutants in the discharged water meet the water quality standard of sewage discharged into urban sewers, and the problem of secondary pollution to the environment caused by wastewater generated by a sludge hydrothermal carbonization technology is solved.

The invention belongs to the technical field of feces treatment, particularly relates to integrated rural toilet feces deep separation treatment equipment, and aims to solve the problems that existing equipment is poor in operation stability and not ideal in total phosphorus and ammonia nitrogen pollutant removal rate, the integrated rural toilet feces deep separation treatment equipment comprises a solid-liquid separation module, an anaerobic treatment tank, an aerobic treatment tank and a liquid-phase flocculation unit, a solid phase and a liquid phase of feces are separated through the solid-liquid separation module, the liquid phase is flocculated through the liquid phase flocculation unit, the liquid phase and the solid phase are put into corresponding fermentation areas of the anaerobic treatment tank to be subjected to anaerobic fermentation treatment, the liquid phase is conveyed to the aerobic treatment tank to be subjected to further aerobic treatment, and the liquid phase and the aerobic treatment tank are subjected to anaerobic fermentation treatment. The integrated treatment of solid-liquid separation, anaerobic biogas production and aerobic denitrification of rural toilet feces is realized, the solid phase and the liquid phase after the integrated treatment can be respectively subjected to further resource utilization, and the system is extremely suitable for being used in rural areas.

The invention belongs to the field of microorganisms, and particularly relates to a high-efficiency denitrification heterotrophic nitrification-aerobic denitrification klebsiella and a culture method and application thereof.A biological material of the high-efficiency denitrification heterotrophic nitrification-aerobic denitrification klebsiella is named as Klebsiella oxytoca CT4 and is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on July 21, 2025, the preservation number of the Klebsiella oxytoca CT4 is CGMCC NO.9687, and the preservation number of the Klebsiella oxytoca CT4 is CGMCC NO.987. The preservation address is Institute of Microbiology, Guangdong Academy of Sciences, on the fifth floor of building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou, Guangdong Province; the preservation number of the strain is GDMCCNO: 66723; according to the strain, the ammonia nitrogen removal rate can reach 98.7% through nitrification-denitrification, the nitrite accumulation amount in the whole process is lower than 0.5 mg / L, and accumulation of toxic intermediate products is avoided. The strain has wide environmental adaptability, can keep efficient denitrification activity under the conditions that the pH is 5.0-10.0 and the C / N ratio is 5-30, can tolerate fluctuation of wastewater quality, does not need to repeatedly adjust the pH value when being used for treating aquaculture wastewater with a high C / N ratio (greater than 20), and can still stabilize the total nitrogen removal rate to be 80% or above, so that the addition cost in a conventional process is remarkably reduced.

Provided are an aerobic denitrification actinomycete strain and a use thereof. The strain is named Streptomyces venezuelae D18, and the aerobic denitrification actinomycete strain can be used for water body remediation. The aerobic denitrification actinomycete strain has a dissolved organic carbon removal rate of 93.34%, a nitrate removal rate of 99.79%, and a total nitrogen removal rate of 98.44%, and the strain has good denitrification performance, and provides a new insight for developing diversified efficient aerobic denitrification biological resources. When the strain is used for water body remediation, the total nitrogen removal rate can reach 89.16%, and the permanganate removal rate can reach 91.44%. The strain can efficiently realize remediation of lightly polluted water bodies.

The invention relates to the technical field of sewage treatment, in particular to application of a signal molecule C8-HSL in strengthening sewage treatment of algal-bacterial symbiotic granular sludge. In-depth study finds that the signal molecule C8-HSL can improve the TN removal rate of the system; according to the present invention, with the application of the signal molecules, the flora composition is changed, the enrichment of denitrification functional bacteria such as Hydrogenphaga, Pseudoxanthobacteria and the like is promoted, and the abundance of the complex organic matter degrading bacteria Desulfomicrobe is enhanced; meanwhile, due to the increase of denitrifying bacteria such as Hydrogenphaga, Thauera and Zoogloea, the variety of species is optimized, and the nitrogen removal efficiency and the organic matter degradation efficiency are improved; based on PICRUSt2 analysis, the signal molecule C8-HSL can increase the abundance of the napAB gene and promote aerobic denitrification; expression of nirK and nirS genes is enhanced, and nitrite is promoted to be converted into nitrogen; meanwhile, the signal molecule C8-HSL effectively improves the denitrification performance of the system by optimizing a nitrogen metabolism pathway.

The utility model belongs to the technical field of biological denitrification of industrial aquaculture water, and particularly relates to a denitrification device suitable for an industrial aquaculture system. Comprising an aerobic nitrification zone, a carbon source zone, an aerobic denitrification zone and a suspended particle removal zone which are communicated in sequence, a filter material is arranged in the aerobic nitrification zone, and the aerobic nitrification zone is used for carrying out biological oxidation on aquaculture water to generate nitrate; the upper part of the carbon source region is filled with carbon sources; the aerobic denitrification area is used for reducing nitrate in the water body into nitrogen; the suspended particle removal area is used for removing suspended particles in the water body. A water inlet pipe is arranged at the bottom of one side wall of the aerobic nitrification area, an aerobic nitrification area overflow port is formed in the upper part of the other side wall of the aerobic nitrification area, and the internal space of the aerobic nitrification area is distributed in a shape; a water outlet pipe is arranged at the bottom of the side wall of the suspended particle removal area. According to the device, aerobic nitrification, aerobic denitrification and suspended particulate matter removal are organically integrated, intensification and modularization are realized, the device is simple in structure and convenient to operate, and the culture denitrification effect is effectively improved.

The invention relates to a high-efficiency phenol degrading bacterium with an aerobic denitrification characteristic and application thereof, and belongs to the technical field of coking wastewater treatment and microorganisms. The strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M20242868, and the preservation time is December 20, 2024. The strain can grow and breed by taking phenol as a unique carbon source, complete degradation of 1250mg / L phenol is realized, and phenol with the concentration range of 1000mg / L can be rapidly degraded within 36 hours. The strain PD-1 can also realize efficient and synchronous removal of phenol and a nitrogen source under the condition that NH4 < + >-N, NO2 <->-N or NO3 <->-N is used as the only nitrogen source. The strain PD-1 provided by the invention can quickly degrade high-concentration phenol in a water body, has the advantages of high efficiency, low energy consumption and no secondary pollution, and has important theoretical and application values.

The invention relates to the technical field of desulfurization and regeneration of waste rubber, in particular to a regeneration and desulfurization method of waste rubber, which comprises the following steps: soaking waste rubber powder in an ammonium bicarbonate solution, heating to 60 DEG C to enable waste rubber particles to form dense micropores, and adding a heterotrophic nitrification-aerobic denitrification bacteria solution to react with zinc oxide in the waste rubber so as to realize the regeneration and desulfurization of the waste rubber. Zinc oxide in waste rubber is separated through the cell surface adsorption effect, thermococcus thermophilic archaea and waste rubber powder are blended and subjected to a fermentation reaction in a closed environment at the temperature of 90-94 DEG C, a fermentation product can be broken with sulfur bonds in the waste rubber, and due to the fact that the thermococcus thermophilic archaea can survive at the higher temperature, the sulfur bonds in the waste rubber can be broken. The reaction is more violent than that of strains surviving at normal temperature, the fermentation product can better penetrate through the waste rubber particles, the fermentation product can more completely break sulfur bonds of the waste rubber particles, and the tensile strength and the elongation at break of the desulfurized rubber are greatly improved.

The invention belongs to the technical field of microorganisms and aquaculture, and discloses a high-environmental-adaptability nitrification and denitrification bacillus cereus strain, a microbial agent and application of the high-environmental-adaptability nitrification and denitrification bacillus cereus strain, the high-environmental-adaptability nitrification and denitrification bacillus cereus strain is bacillus cereus HCCN1 and is preserved in Guangdong Microbial Culture Collection Center on November 13, 2024, and the preservation number is GDMCC No: 65477. The invention also discloses a microbial agent containing the strain HCCN1 as an active component and application of the microbial agent. The strain HCCN1 has high stress resistance and high environmental adaptability, has heterotrophic nitrification and aerobic denitrification functions at the same time, can efficiently degrade nitrogen and phosphorus under the severe environmental conditions of wide pH value range, temperature range, high salt and alkali concentration and the like, performs purification treatment on aquaculture water, and is high in biological safety and low in cost. The industrial requirements of efficient, healthy and sustainable aquaculture can be met.

The invention discloses a combined enhanced denitrification device and method based on a magnetic field-modified polyurethane composite material, the device comprises a water inlet area, a gravel filler area, an aeration area, a polyurethane filler tray area, a sludge discharge area, a water outlet area and a PLC controller, nZVI-coated SiO2, gravel and a strong magnet are configured in the gravel filler area, anaerobic denitrification is carried out on micro-polluted water under the action of a strong magnetic field, and then the micro-polluted water is subjected to anaerobic denitrification under the action of the strong magnetic field; degrading part of organic matters and nitrate; an aeration disc is arranged in the water inlet area for back flushing; and a modified polyurethane sponge filler plate is arranged in the polyurethane filler plate area, and is used for carrying out aerobic denitrification and microbial denitrification on the aerated and oxygenated water body to generate sludge precipitate. The problems that an inorganic electron donor is unstable in release speed, poor in synergistic effect with microorganisms and the like are solved, flora loss is avoided, the later biofilm formation time is shortened, the biological activity of the microorganisms is promoted, the enhanced nitrogen removal process of the water body is more effectively promoted, and efficient nitrogen removal of the micro-polluted water body is achieved.

An Aspergillus sp. DH4 with aerobic denitrification enhanced by an inorganic electron donor and its application are provided, which relates to the field of microbial technologies. The Aspergillus sp. DH4 is preserved at China Center for Type Culture Collection (CCTCC), a preservation address is Wuhan University, 299 Bayi Road, Wuchang District, Wuhan City, China, a preservation number is CCTCC NO: M20232690, and a preservation date is Dec. 27, 2023.

The invention relates to the technical field of environmental microbial resource development and sewage denitrification. The invention discloses an efficient denitrification strain Paracoccus sp.N21 with a flocculation characteristic, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34710. The invention further discloses a preparation method of the efficient denitrification strain Paracoccus sp.N21. The strain obtained by the invention can completely remove ammonia nitrogen within 12 hours and thoroughly remove nitrate nitrogen within 48 hours, the removal rate reaches 100%, and ammonia nitrogen is preferentially degraded in a mixed nitrogen source. The method has the unique advantages that the flocculation rate in the nitrification process and the flocculation rate in the denitrification process exceed 93.9%, and the usage amount of a flocculating agent can be greatly reduced. When actual kitchen wastewater is treated, the ammonia nitrogen removal rate reaches 100%, and the removal rate reaches up to 7.51 mg / (L.h). The application of the strain can effectively reduce the volume of a reactor, reduce energy consumption and operation cost, provides a new solution for simplifying a biological denitrification process, is particularly suitable for treating high-ammonia-nitrogen kitchen wastewater, and has remarkable environmental and economic benefits.

The present invention belongs to the field of microbial technology, and discloses a low-temperature-tolerant aerobic denitrifying bacterium and its application; the low-temperature-tolerant aerobic denitrifying bacterium is Enterobacter ludwigii CW-01, and its preservation number is CGMCC No. 27126. The Enterobacter ludwigii CW-01 strain of the present invention can efficiently achieve aerobic denitrification to degrade ammonia nitrogen, nitrate, and nitrite within the range of 4-35 °C, and is particularly suitable for wastewater treatment where it is difficult to remove ammonia nitrogen, nitrate, and nitrite due to low temperature; this strain also has good salt tolerance, providing a good microbial material for the biological denitrification treatment of low-temperature, high-salt, and high-ammonia-nitrogen polluted water bodies; moreover, while efficiently removing nitrogen, it also significantly reduces the usage amount of carbon sources, greatly reducing the costs of enterprises, and having good application prospects in the field of sewage treatment.

The invention relates to the field of microbial technology, and discloses a strain with low-temperature resistance and aerobic denitrification function and an application thereof. The strain is named Pseudomonas sp. BWFA71, and the preservation unit is Guangdong Microbial Culture Collection Center (GDMCC), the preservation date is February 22, 2022, and the preservation number is GDMCCNO:62257. The strain of the invention has strong applicability and a wide range of temperature tolerance. The maximum and minimum tolerance temperatures are both wider than those of the same genus strains in the prior art. The strain can have good denitrification ability under conditions of 6 to 30°C, and the dissolved oxygen in the water has no obvious effect on its denitrification process. The strain is particularly suitable for sewage treatment and denitrification systems in areas with relatively large temperature changes, and for total nitrogen control in ecological restoration projects such as aquaculture water bodies and landscape water bodies where anaerobic denitrification cannot be performed.

The invention discloses Pseudomonas tai H1R1 and application thereof in treatment of mariculture tail water, the Pseudomonas tai H1R1 has excellent high salt resistance and aerobic denitrification function, can directly convert ammonia nitrogen and nitrate into nitrogen and efficiently remove nitrogen, the nitrogen removal rate can reach 80% or above, nitrogen load in water is reduced, the nitrogen removal efficiency is improved, and the application of the Pseudomonas tai H1R1 in treatment of mariculture tail water is improved. The problem of water eutrophication caused by nitrogen can be prevented; the system can survive and breed under various severe environment conditions and treat mariculture tail water. The pseudomonas tai H1R1 disclosed by the invention can utilize organic matters in mariculture tail water as a carbon source, so that the growth speed and the growth amount of bacteria are increased, and organic wastes in a culture process are reduced, so that the strain H1R1 can promote self growth while treating the mariculture tail water; and double benefits of environmental governance and resource cyclic utilization are realized.

The invention relates to the field of microorganisms, and discloses a stress-resistant heterotrophic nitrification-aerobic denitrification nitrogen removal bacterium and application thereof. The pseudomonas is named as H1, the microorganism classification name of the pseudomonas is Pseudomonas sihuiensis, the preservation number of the pseudomonas is CGMCC (China General Microbiological Culture Collection Center) NO: 33481, the preservation date of the pseudomonas is January 20, 2025, and the preservation place of the pseudomonas is China General Microbiological Culture Collection Center. The pseudomonas H1 disclosed by the invention can still realize efficient denitrification in a heterotrophic nitrification-aerobic denitrification way in a high-salt and high-nitrogen water body environment containing toxic pollutants with benzene ring functional groups, so that the pseudomonas H1 has great application potential in biological treatment of nitrogen-containing wastewater.

The present invention belongs to the field of sewage treatment, and specifically relates to a micro-aerobic denitrification wastewater treatment process. Bacillus is introduced into a homogenizing tank, and a biological activation complex is added; the wastewater enters the homogenizing tank and is evenly mixed with the return sludge and the mixed liquid, and then enters a high-efficiency denitrification reactor; the dissolved oxygen in the micro-aerobic biochemical tank is gradually reduced from the water inlet to the water outlet, and the effluent of the high-efficiency denitrification reactor enters the micro-aerobic biochemical tank for further reaction; the nitrification liquid is refluxed to the high-efficiency denitrification reactor and the micro-aerobic biochemical tank, and the sludge after precipitation in the sedimentation tank is discharged to the sludge tank, and refluxed to the high-efficiency denitrification reactor and the micro-aerobic biochemical tank, and the wastewater that meets the standards is discharged. The process of the present invention can realize synchronous short-range nitrification and denitrification in the system, solves the problem of biological phase interference caused by the reflux of the nitrification liquid of the traditional activated sludge method, and at the same time greatly reduces the amount of additional carbon source required for denitrification compared with the traditional process.

The invention relates to the technical field of microorganisms, in particular to acinetobacter YYL-1 and application, and the preservation number of the acinetobacter YYL-1 is CGMCC (China General Microbiological Culture Collection Center) No.28247. The acinetobacter YYL-1 has heterotrophic nitrification-aerobic denitrification one-stop denitrification performance, can realize high-efficiency removal of ammonia nitrogen by a single bacterium in a medium and low temperature environment, especially can realize effective colonization in activated sludge, and enhances the denitrification performance of the activated sludge in a low temperature environment.

The invention belongs to the technical field of microorganisms and aquaculture, and discloses a Bacillus cereus strain with high environmental adaptability for nitrification and denitrification, a microbial agent and its application. Bacillus cereus HCCN1 was deposited in the Guangdong Provincial Microbial Strain Collection Center on November 13, 2024, with a deposit number of GDMCC No: 65477. The present invention also discloses a microbial agent comprising the strain HCCN1 as an active ingredient and its application. The strain HCCN1 of the present invention has high stress resistance and high environmental adaptability, and has heterotrophic nitrification and aerobic denitrification functions. It can efficiently degrade nitrogen and phosphorus under harsh environmental conditions such as a wide pH range, temperature range, and high salt and alkali concentrations, and purify aquaculture water bodies. It has high biosafety and can meet the industry needs of efficient, healthy and sustainable aquaculture.

The invention discloses Pseudomonas hunanensis N6-2 and application thereof, and belongs to the technical field of environmental microorganisms, the Pseudomonas hunanensis N6-2 is separated from the wetland of the Yellow River Delta, can grow by taking ammonia nitrogen, nitrate nitrogen and nitrite nitrogen as a unique or mixed nitrogen source, and has the functions of heterotrophic nitrification and aerobic denitrification at the same time. The strain has very high treatment efficiency on sewage with the nitrogen concentration of 500 mg / L or below, can tolerate high-salt-alkali sewage with the salinity of 50 g / L and the pH value of 10, has wide environmental adaptability, is non-toxic and harmless in the treatment process and eco-friendly, and has great application potential.

A low-temperature-resistant heterotrophic nitrification and aerobic denitrification bacteria is used for wastewater denitrification method, relates to a kind of biological bacteria for wastewater denitrification method, the bacteria is Pseudomonas umsongensis BK17, belongs to Pseudomonas, is preserved in China typical culture preservation center, and the preservation number is CCTCC M 20242924, and the preservation date is December 27, 2024.The strain is a heterotrophic nitrification-aerobic denitrification bacteria, can efficiently remove inorganic nitrogen pollution in water body, and still has good denitrification performance under low temperature stress, and has strong low temperature tolerance.The strain can efficiently treat nitrogen-containing wastewater at 10 DEG C, and the removal efficiency can reach more than 99%.In the inorganic nitrogen pollution treatment and application of various water bodies such as domestic sewage in cold regions of China, it has great potential.

The invention relates to the technical field of soil conditioners and applications thereof, and discloses a soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum and an application thereof in ploughing ability improvement, the soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum comprises an organic pollutant degradation functional flora, a soil carbon sequestration functional flora and a small molecular organic matter production functional flora; the organic pollutant degrading functional flora comprises efficient pollutant degrading bacteria; the soil carbon sequestration functional flora comprises ammonia oxidizing bacteria, ammonia oxidizing archaea, complete ammonia oxidizing bacteria, sulfate reducing bacteria, iron reducing bacteria, rhizobium and photosynthetic flora; the micromolecular organic matter production functional flora comprises heterotrophic nitrification-aerobic denitrification bacteria, zoogloea, phosphorus-accumulating bacteria and photosynthetic bacteria. According to the scheme, multiple florae are specifically arranged in the synergistic agent, and through the synergistic interaction effect of the florae, the effects of the synergistic agent in multiple fields of soil carbon sequestration, pollution reduction and yield increase are effectively improved.