52 results about "Providencia" patented technology

The invention discloses an ammonia nitrogen-removed compound microorganism microbial agent. The ammonia nitrogen-removed compound microorganism microbial agent is prepared from Providencia rettgeri, Microbacterium lactium, Lactobacillus delbrueckii and Rhodococcus coprophilus in a volume ratio of 5 to 2.5 to 1 to 5. The invention further discloses application of the compound microorganism microbial agent in ammonia nitrogen-containing wastewater treatment. The four bacteria are reasonably matched and are combined by virtue of an adsorption method and an embedding method to form immobilized cell particles, so that the ammonia-nitrogen degradation capacity and stability of the compound microorganism microbial agent are remarkably enhanced; a dominant bacterial community can be immediately formed after the compound microorganism microbial agent is applied to denitrification and treatment process of sewage, river way landscape water and the like, the treatment time is short, and ammonia and nitrogen indexes of the sewage are rapidly decreased; and meanwhile, the compound microorganism microbial agent has an aerobic denitrification function and further has relatively good effect of removing COD, and accumulation of nitrite and nitrate is avoided.

The invention discloses a Providencia rettgeri strain and application thereof. The Providencia rettgeri YL is collected in China General Microbiological Culture Collection Center (CGMCC) on April 28th, 2013, and the collection number is CGMCC No.7544. The Providencia rettgeri is used for treating nitrogenous organic wastewater, and can effectively remove organic nitrogen, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen while removing organic carbon, and thus, the whole organic carbon and nitrogen synchronous removing process can be implemented only by one aerobic stage. The strain has the heterotrophic nitrification-aerobic denitrification function, can synchronously remove the organic carbon and nitrogen under aerobic conditions, can greatly reduce the occupied area and construction investment, can shorten the system start-up time, reduces the treatment cost, and enhances the treatment efficiency.

The invention discloses a root-knot nematode biocontrol bacterium, an antibiological inoculant and application of the biocontrol bacterium and the antibiological inoculant. The biocontrol bacterium is a Providencia rettgeri strain UN06, and the preservation serial number of the biocontrol bacterium is CGMCC No. 10246. Fermentation liquor obtained through fermentation of the Providencia rettgeri strain UN06, adsorbing carriers such as mushroom dreg and grass carbon, and nutrient substance urea required by bacterium growth are evenly mixed to be granulated to form UN06 granules. The control efficiencies of 100 kilograms of the applied UN06 granules in each mu to tomato root-knot eelworms, cucumber root-knot nematodes and tobacco root-knot nematodes are 90.17 percent, 88.66 percent and 85.98 percent. The root-knot nematode antibiological inoculant has the advantages of being low in cost, high in control efficiency, free of residual and safe to people and livestock, and is an efficient biopesticide for preventing and treating root knot nematode diseases.

Belonging to the technical field of enzyme preparations and wine brewing additives, the invention relates to a preparation method and application of a natural material composite system immobilized bifunctional enzyme used for wine. The preparation method includes: subjecting the free bifunctional enzyme that is extracted from Providencia sp. JNB815 and has the activity of degrading urea and ethyl carbamate (EC) to chitosan adsorption, gelatin embedding and genipin crosslinking, thus obtaining the immobilized bifunctional enzyme. When the immobilized enzyme takes urea as the substrate, the optimum temperature is 40DEG C, and the optimum pH is 4.0; when EC is adopted as the substrate, the optimum temperature is 60DEG C, and the optimum pH is 4.5. The storage half-life periods of two enzyme activity are 54d and 45d respectively. The invention also relates to application of the immobilized bifunctional enzyme, which can reach a urea removal rate of 93.03% and an EC removal rate of 56.60%. According to the invention, the process conditions of original fermented wine cannot be changed, the wine flavor is not changed, and the immobilized bifunctional enzyme can be reused, and has more advantages in the application of removing trace urea and EC from yellow wine.

The invention discloses a strain for degrading a pendimethalin pesticide and a fungicide and an application of the strain. The strain is Providencia rettgeri JZB42C003 which is preserved at CGMCC on 17th, November, 2014, wherein the preservation number is CGMCC No. 9977. The JZB42C003 strain is good in degrading effect on pendimethalin or beta-cypermethrin left in soil, fruits, vegetables or water bodies, and the degradation rate on 50mg / L pendimethalin in a basic inorganic salt culture solution treated by the JZB42C003 strain in 5 days can reach 68.5%, and the degradation rate on 50mg / L beta-cypermethrin in the basic inorganic salt culture solution treated by the JZB42C003 strain in 5 days can reach 55.7%.

The invention relates to a microbe composite bacterial liquid comprising live bacteria of clostridium thermopalmarium, clostridium thermosuccinogenes, brevibacillus borstelensis, paenibacillus barengoltzii, bacillus subtilis, pseudomonas mendocina, and providencia rettgeri. The invention also provides a bacterial preparation prepared by using the composite bacterial liquid. The bacterial preparation is used for preparing an organic fertilizer. According to the invention, with the composite microbe bacterial liquid, during a composite fertilizer utilization process, the dosage of inorganic composite fertilizer can be reduced, such that production cost can be reduced. Also, with a synergetic effect provided by the microbe bacterial preparation, when dosages of nitrogen, phosphorous and potassium in organic-inorganic composite fertilizers are low, agricultural and forestry waste stalks with low prices and rich organic substances such as nitrogen, phosphorous, potassium and calcium are converted into micro-molecular substances such as monosaccharide and oligosaccharide which can be utilized by plants. Therefore, a relatively high crop yield is obtained, and a substantial yield increasing effect is provided. Agriculture in our nation has a severe dependence on chemical fertilizers. With the composite bacterial liquid provided by the invention, the situation can be changed, and agricultural ecological protection can be enhanced.

The invention discloses providencia vermicola and application of providencia vermicola in preparing antibacterial drugs. The preservation number of providencia vermicola No. 22 is CCTCC No. M2013696. The providencia vermicola No. 22 has antagonism, so that the providencia vermicola No. 22 can be used for preparing preparation for preventing and controlling vibrio alginolyticus or preparing assistant hemolysis vibrio preparation; the preparation for preventing and controlling vibrio alginolyticus or the assistant hemolysis vibrio preparation can be used for beneficial microbial preparation to replace chemical medicine, improves water quality, and eliminates vibrio alginolyticus or assistant hemolysis vibrio in an aquatic product cultivation system, so that damage to aquatic product cultivation objects from the vibrio alginolyticus or assistant hemolysis vibrio is prevented, the development of the aquatic product cultivation industry is promoted, aquatic products are prevented from carrying the vibrio alginolyticus or assistant hemolysis vibrio, and health and safety of human beings are ensured.

The invention provides provindencia rettegeri Bg-7, and the preservation number of provindencia rettegeri Bg-7 is CGMCC No.9077. The provindencia rettegeri Bg-7 is Gram-negative bacterium, the contact enzyme reaction is positive, the oxidase reaction is negative, provindencia rettegeri Bg-7 can form milk-white bacterium groups on a MA culture medium (Difco, article number: 212185), the bacterium group is in a round shape, and the middle of the bacterium group projects slightly. The reaction that providencia rettgeri Bg-7 utilizes citrate to react is positive, and esculin iron citrate, 2-ketopotassium gluconate, mannitol, inositol, and glucose are fermented to produce acids. When the provided provindencia rettegeri Bg-7 is co-cultured with paralembus digitiformis of sea fishes, provindencia rettegeri Bg-7 can kill at least 97% of paralembus digitiformis in the culture system, and a novel method is provided for controlling sea fish diseases caused by paralembus digitiformis.

The invention relates to an oil-containing sewage degradation compound bacterium. The compound bacterium mainly solves the problems that an existing biological bacterial agent only has effects on oneindex of oil-containing sewage and is relatively high in environmental requirement. The oil-containing sewage degradation compound bacterium comprises providencia rettferi, morganella morganii and pseudomonas aeruginosa in the volume ratio of 1:1:1. A preparation method includes the steps that the three strains are cultured in an LB culture medium respectively for 48 h at 37 DEG C, a bacteria solution of each strain and glycerin are mixed, placed in an aseptic centrifuge tube and stored at a low temperature, the mixtures are placed to be at room temperature before use and are activated in theLB culture medium, the strains are diluted to the same magnitude order by using sterile normal saline according to the total number of bacterial colonies, prepared bacterium suspensions are fully mixed in proportion, and the mixed bacterial agent is obtained. The oil-containing sewage degradation compound bacterium can be used for simultaneously degrading oil and COD and can adapt to more severe treatment environments.

The invention discloses a high-efficiency denitrification compound microbial inoculum, and preparation and an application thereof, and belongs to the field of biotechnology. The microbial inoculum forwastewater treatment mainly includes six strains including nitrobacter sp, nitrosomonas sp, alcaligenes aquatilis, paracoccus nitrovorans, bacillus cereus, providencia vermicola and the like. A culture method includes enrichment, purification and enlarged culture of bacteria; the used microbial inoculum has the advantages of high denitrification efficiency, short domestication time, salt tolerance and the like, and is suitable for large-scale production. The obtained microbial inoculum can effectively treat high ammonia-nitrogen wastewater. The microbial preparation still has good denitrification ability under the condition of 2% total salt, solves the limitation of high-salt environment on a traditional biological denitrification method, and has broad application prospect and good socialbenefits.

The invention discloses providencia for synthesizing ethanol-resistant urethanase and urease, and belongs to the technical field of bioengineering. A bacterial strain obtained by separation is identified as the providencia, and the synthesized urethanase and urease still have efficient enzyme activity under high-concentration ethanol (20 percent v / v) and acidic conditions (pH 4.5). Therefore, the application potential is huge in the removal of ethyl carbamate and urea in yellow wine and other fermented food, accordingly the control on the ethyl carbamate from a source to an end is realized, and the economical and social benefits are huge.

The invention discloses a strain Pr-nj01 having insecticidal activity, an insecticidal agent and application thereof. The strain Pr-nj01 is identified as Providencia rettgeri, is preserved in the China center for type culture collection on May 10, 2017 and has a preservation number of CCTCC NO: M2017251. The separated and screened strain Pr-nj01 having insecticidal activity can be used for preventing and treating pests in fields, has broad-spectrum killing effects on pests in fields, can kill lepidoptera and hemiptera pests in fields and has good killing effects. The insecticidal agent is prepared through simple processes and has the advantages of low production cost, use convenience and good insecticidal effects. Through use of the insecticidal agent, chemical pesticide is reduced. The strain Pr-nj01 has the important significance for protecting the ecological environment and ensuring food safety.

The invention relates to a bacterium with insecticidal activity. The Providencia Ewing water aqua has the effective active component of Providencia Ewing, and is characterized by being a biocontrol bacterium water aqua. The Providencia Ewing water aqua comprises the following components in percentage by weight: 2*10<9> cfu / mL of Providencia Ewing, 0.5% of NNO, 5% of sodium dodecyl benzene sulfonate (SDBS) and 0.1% of sodium benzoate. During application, the Providencia Ewing water aqua is sprayed onto the surface of a plant for controlling asparagus caterpillars. The Providencia Ewing water aqua has the advantages as follows: through introduction of the environment-friendly pest biocontrol bacterium Providencia Ewing, the asparagus caterpillars are controlled, so that the using amount of chemicals is reduced, the cost of agriculture production is reduced and improvement on the ecological environment is facilitated.

Oligonucleotide probes for detecting Enterobacteriaceae species. Unique gyrA coding regions permit the development of probes specific for eight different species: Escherichia coli, Citrobacter freundii, Enterobacter aerogenes, Enterobacter cloacae, Klebsiella oxytoca, Klebsiella pneumoniae, Providencia stuartii and Serratia marcescens. The invention thereby provides methods for the species-specific identification of these Enterobacteriaceae in a sample, and detection and diagnosis of Enterobacteriaceae infection in a subject. Further, nucleic acids are provided for determining quinolone-resistant status of these Enterobacteriaceae.

The invention discloses strawberry high-efficiency foliar bacterial fertilizer and a preparation method and application thereof. The fertilizer comprises 10-20 parts of stenotrophomonas maltophilia microbial inoculum, 8-12 parts of Providencia stuartii microbial inoculum, 3-7 parts of monospora microbial inoculum, 8-12 parts of chitosan, 6-10 parts of amino acid, 6-10 parts of algin, 10-20 parts of microelement fertilizer and 20-40 parts of water. The fertilizer is applied to the aspects of improving the yield of strawberries, improve the quality of the strawberries, improving the storage performance of the strawberries, inducing the improvement of the disease resistance of the strawberries, promoting the growth of strawberry plants and the like. The fertilizer has the advantages of beingsignificant in effect, green, free from pollution, high in fertilizer utilization rate, low in cost and easy to be popularized and the like, diseases and pests can be prevented and controlled, and theyield and the quality are improved.

The invention discloses an efficient bacterial foliage fertilizer for tea trees as well as a preparation method and application of the fertilizer. The fertilizer comprises 10-20 parts of a stenotrophomonas maltophilia agent, 8-12 parts of a providencia stuartii agent, 8-12 parts of a monospora agent, 3-7 parts of chitosan, 8-12 parts of amino acids, 3-7 parts of cytex, 10-20 parts of a micro-fertilizer and 20-40 parts of water. The fertilizer is applied for increasing the yield of the tea trees, improving the quality of the tea trees, inducing to enhance the disease resistance of the tea trees, and promoting growth of low-age and adult tea tree plants. The efficient bacterial foliage fertilizer disclosed in the invention has the advantages of being obvious in effect, green, free of pollution, high in fertilizer utilization rate, capable of controlling diseases and improving the yield and quality, low in cost, easy to popularize and the like.

The invention relates to a composite micro-ecological preparation for preventing and controlling acute hepatopancreatic necrosis syndrome (AHPNS) of Pacific white shrimps and application of the composite micro-ecological preparation. The preparation comprises providencia vermicola and enteric bacilli. The composite micro-ecological preparation is applied together with feed. The composite micro-ecological preparation provided by the invention has a good effect in inhibiting Vibrio parahemolyticus, has a remarkable application effects, is one of significant ways for green breeding of the Pacificwhite shrimps and has good application prospects.

The invention discloses a method for degrading antibiotics in biogas slurry by the aid of ultraviolet irradiation processes. The method includes adding Providencia rettgeri solution into the biogas slurry and preliminarily degrading the antibiotics; adding persulfate and ferrous sulfate, forming advanced oxidation systems and then starting ultraviolet irradiation; carrying out oxidation / light degradation and continuing to degrade the antibiotics in the biogas slurry; adding dithiothreitol solution and recycling the advanced oxidation systems; carrying out secondary oxidation / light degradationand degrading the antibiotics. The method has the advantages that the antibiotics in the biogas slurry can be degraded by the aid of technologies, and the method is high in degradation speed and degradation efficiency; good degradation effects can be realized, the antibiotics can be degraded into small-molecular substances, materials used in degradation procedures are low in cost and can come fromextensive sources, and the antibiotics in the biogas slurry can be effectively degraded.

The invention discloses a highly-efficient foliar bacterial fertilizer for a plum tree, and a preparation method and an application thereof. The highly-efficient foliar bacterial fertilizer comprises10 to 20 parts of a stenotrophomonas maltophilia preparation, 8 to 12 parts of a Providencia stuartii preparation, 3 to 7 parts of a monospora preparation, 6 to 10 parts of chitosan, 6 to 10 parts ofamino acid, 8 to 12 parts of cytex, 10 to 20 parts of a microelement fertilizer and 20 to 30 parts of water. The highly-efficient foliar bacterial fertilizer provided by the invention is applied to the following aspects: increase of the yield of plums, improvement of the quality of the plums, enhancement of the storage performance of the plums, induction of reinforcement of disease resistance of the plum tree, promotion of the growth of a plum tree plant, etc. The highly-efficient foliar bacterial fertilizer provided by the invention has the advantages of obvious effects, greenness, no pollution, high fertilizer utilization rate, prevention and control of diseases and pests, yield and quality improvement, low cost, easiness in popularization, etc.

The invention discloses an efficient bacterial leaf fertilizer for Chinese herbal medicine and a preparation method and application thereof. The efficient bacterial leaf fertilizer contains 10-20 parts of stenotrophomonas maltophilia agent, 8-12 parts of providencia stuartii agent, 8-12 parts of single-spore microbial agent, 3-7 parts of chitosan, 8-12 parts of amino acid, 3-7 parts of cytex, 10-20 parts of micro element fertilizer and 20-30 parts of water. The efficient bacterial leaf fertilizer is applied to the aspects such ass increase of Chinese herbal medicine yield, improvement of Chinese herbal medicine quality, induction and improvement of disease resistance of Chinese herbal medicine and promotion of Chinese herbal medicine plant growth and has the advantages of being remarkablein effect, environmentally friendly, free of pollution, high in utilization rate, low in cost and easy to popularize, preventing and controlling diseases and pests, improving yield and quality and thelike.

The invention discloses a preparation method and application of a providencia stuartii enzyme agent. According to the preparation method, with providencia stuartii CCTCC M 2017738 as a strain, the providencia stuartii enzyme agent is prepared through expanding propagation of a bacterial suspension and ultrasonic crushing extraction of a degrading enzyme agent. The degrading enzyme agent can be applied to efficient degradation of dimethachlon, vinclozolin and iprodione in plants such as oilseed rape, Chinese cabbage, purple pakchoi, lettuce, fragrant-flowered garlic, cucumbers, tomatoes, peppers, potatoes and tobaccos, soil and water. The providencia stuartii enzyme agent has the advantages of being good in quick-acting performance, environmentally friendly, capable of achieving an obviouseffect, low in cost, easy to popularize and the like.

The invention discloses an efficient leaf surface bacterial fertilizer for kiwi fruits as well as a preparation method and application of the efficient leaf surface bacterial fertilizer. The efficientleaf surface bacterial fertilizer contains the following components in parts by weight: 10-20 parts of a stenotrophomonas maltophilia bacterial agent, 8-12 parts of a Providencia stuartii bacterial agent, 3-7 parts of a monospora bacterial agent, 8-12 parts of chitosan, 8-12 parts of amino acid, 8-12 parts of algin, 10-20 parts of a microelement fertilizer and 20-40 parts of water. The efficientleaf surface bacterial fertilizer is applied to the aspects of increasing the yield of the kiwi fruits, improving the quality and storage performance of the kiwi fruits, inducing the disease resistance of the kiwi fruits, promoting the growth of low-age and forested kiwi fruit plants, and the like. The efficient leaf surface bacterial fertilizer has the advantages that the effect is remarkable, the fertilizer is environment-friendly, pollution-free, high in utilization rate, capable of preventing and controlling diseases, low in cost and easy to popularize, the yield of the kiwi fruits is increased, the quality of the kiwi fruits is improved, and the like.

The invention discloses a preparation method and an application of a Providencia stuartii immobilized bacterium. The immobilized bacterium takes Providencia stuartii as a strain and takes sodium algaacid and bamboo charcoal as immobilized materials, and the providencia stuartii is effectively embedded to prepare the immobilized bacterium. The collection number of the Providencia stuartii strain is CCTCC M2017738. The immobilized bacterium can be applied to high-effective degradation of dimethachlon, vinclozolin and iprodione in soil and water. The immobilized bacterium has the advantages of good persistence, environmental friendliness, remarkable effect, low cost, easiness in popularization and the like.

The invention provides application of Providencia in production of hydroxyethyl piperazine ethanesulfonic acid, and belongs to the technical field of biosynthesis. According to the method disclosed by the invention, the isethionesulfonic acid is produced by biotransformation of isethionesulfonic acid from isethionesulfonic acid and sodium chloroethanesulfonate by adopting Providencia, no organic reagent is used in the transformation process, normal-pressure operation can be carried out, the reaction condition is mild, the purity of isethionesulfonic acid obtained by transformation is high, and the yield of a transformation product subjected to purification treatment and HPLC detection reaches 99% or above, and the purity is greater than or equal to 99% (HPLC).

The invention relates to a nucleic acid reagent and a digital PCR (polymerase chain reaction) kit for detecting providencia. The nucleic acid reagent comprises primers as shown in SEQ ID NO. 1-6 and probes as shown in SEQ ID NO. 9-11. According to the invention, a detection system for simultaneously detecting the providencia stuartii, the providencia alcaligenes and the providencia reevesii is established; a detection system contains an exogenous internal control as a contrast, and quality control can be carried out on the detection process; the detection result has good sensitivity and specificity.

The invention discloses providencia vermicola and application of providencia vermicola in preparing antibacterial drugs. The preservation number of providencia vermicola No. 22 is CCTCC No. M2013696. The providencia vermicola No. 22 has antagonism, so that the providencia vermicola No. 22 can be used for preparing preparation for preventing and controlling vibrio alginolyticus or preparing assistant hemolysis vibrio preparation; the preparation for preventing and controlling vibrio alginolyticus or the assistant hemolysis vibrio preparation can be used for beneficial microbial preparation to replace chemical medicine, improves water quality, and eliminates vibrio alginolyticus or assistant hemolysis vibrio in an aquatic product cultivation system, so that damage to aquatic product cultivation objects from the vibrio alginolyticus or assistant hemolysis vibrio is prevented, the development of the aquatic product cultivation industry is promoted, aquatic products are prevented from carrying the vibrio alginolyticus or assistant hemolysis vibrio, and health and safety of human beings are ensured.

The invention belongs to the technical field of biological treatment of environmental pollutants, and specifically discloses a high-efficiency Providencia sp. 2D that can completely degrade dibutyl phthalate. The strain was released on January 22, 2015 It was deposited in the China Center for Type Culture Collection (CCTCC) in Japan, and the deposit number is CCTCC M 2015057. The strain degraded more than 80% of the high-concentration DBP (1000 mg / L) in 72 h, and almost completely degraded the low-concentration DBP (200 mg / L); the strain was also able to degrade phthalic acid, an intermediate product of DBP , and eventually DBP is completely mineralized. The strain has high-efficiency DBP degradation ability, which greatly supplements the database of DBP-degrading bacteria. It has a good application prospect.

The invention discloses an efficient leaf surface bacterial fertilizer for blueberries as well as a preparation method and application of the efficient leaf surface bacterial fertilizer. The efficientleaf surface bacterial fertilizer contains the following components in parts by weight: 10-20 parts of a stenotrophomonas maltophilia bacterial agent, 3-7 parts of a Providencia stuartii bacterial agent, 8-12 parts of a monospora bacterial agent, 6-10 parts of chitosan, 8-12 parts of amino acid, 6-10 parts of algin, 10-20 parts of a microelement fertilizer and 20-40 parts of water. The efficientleaf surface bacterial fertilizer is applied to the aspects of increasing the yield of the blueberries, improving the quality and storage performance of the blueberries, inducing the disease resistance of the blueberries and promoting the growth of low-age and forested blueberry plants. The efficient leaf surface bacterial fertilizer has the advantages that the effect is remarkable, the fertilizeris environment-friendly, pollution-free, high in utilization rate, capable of preventing and controlling diseases, low in cost and easy to popularize, the yield of the blueberries is increased, the quality of the blueberries is improved, and the like.