Providencia Ewing water aqua and application thereof

A Providencia bacterium, water agent technology, applied in the direction of application, insecticide, biocide, etc., to achieve the effect of reducing the amount of use, being environmentally friendly, and improving the ecological environment

Inactive Publication Date: 2015-06-03
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Providencia Ewing water aqua and application thereof

Examples

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Effect test

Embodiment 1

[0013] Embodiment 1: described Providencia water agent is to the bioassay of 2 ages and 3 age beet armyworms

[0014] The Providencia bacterium was cultured with shaking at 200 rpm for 2 days at 32 ° C in LB liquid medium, and then diluted to 2 × 10 with sterile water. 10 cfu / mL spare.

[0015] Prepare 500mL solution with sterile water, calculated by weight percentage, which contains 0.5% sodium methylene dinaphthalene sulfonate, 5% sodium dodecylbenzene sulfonate, 0.1% sodium benzoate, and then add the above preparation good Providencia to a final concentration of 2 x 10 9 cfu / mL.

[0016] The bioassay was carried out by spraying the bacterial water agent with artificial feed. The experiments were carried out with 2nd and 3rd instar larvae of beet armyworm respectively. Bioassays were performed in six-well plastic plates. Pick 2nd instar (2nd) beet armyworms of the same day age, 3 heads per hole, two plates as one group, four groups are set up, and feed sprayed with bact...

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Abstract

The invention relates to a bacterium with insecticidal activity. The Providencia Ewing water aqua has the effective active component of Providencia Ewing, and is characterized by being a biocontrol bacterium water aqua. The Providencia Ewing water aqua comprises the following components in percentage by weight: 2*10<9> cfu/mL of Providencia Ewing, 0.5% of NNO, 5% of sodium dodecyl benzene sulfonate (SDBS) and 0.1% of sodium benzoate. During application, the Providencia Ewing water aqua is sprayed onto the surface of a plant for controlling asparagus caterpillars. The Providencia Ewing water aqua has the advantages as follows: through introduction of the environment-friendly pest biocontrol bacterium Providencia Ewing, the asparagus caterpillars are controlled, so that the using amount of chemicals is reduced, the cost of agriculture production is reduced and improvement on the ecological environment is facilitated.

Description

technical field [0001] The invention relates to a biocontrol bacterial water agent with high insecticidal activity against beet armyworm, and belongs to the field of biotechnology. Background technique [0002] The beet armyworm (Spodoptera exigus Hiibner) is a worldwide distribution and intermittent large-scale agricultural pest, which harms many kinds of food and vegetable crops. The production mainly relies on chemical pesticide control. It is urgent to find new control agents and technologies for pollution-free production. [0003] There have been many successful examples of using natural enemy resources to prevent and control rice pests and diseases. For example, use nuclear polyhedrosis virus to prevent and control beet armyworm and Spodoptera litura, etc.; use Braconidia spp. These biological control measures have reduced the use of chemical pesticides to a certain extent, which not only meets people's demand for green and pollution-free production products, but als...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N25/02A01P7/04
Inventor 罗光华姚静王利华张志春刘宝生杨琼郭慧芳方继朝
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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