30 results about "Effective microorganism" patented technology

The invention discloses a mud flat-derived malathion degrading bacterium and application thereof, and relates to the technical field of biology. The malathion degrading bacterium is Ectopseudomonas khazarica FJ5-34-2, and is preserved in the China General Microbiological Culture Collection Center on November 8, 2024, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.32547. The malathion degrading bacterium has the advantages that the malathion degrading bacterium can be used for degrading malathion; according to the invention, an in-vitro pseudomonas strain FJ5-34-2 is screened from a mud flat sample in a new area of Northern Daihe River in Qinhuangdao, Hebei province. The in-vitro pseudomonas can effectively degrade malathion, and the degradation rate of 100 mg / L malathion within 72 hours can reach 94.93%. The invention provides a new effective microorganism for microbial degradation of malathion, which has great practical significance for relieving environmental residues of pesticides.

The invention discloses a leak-free core-shell hydrogel microsphere delivery system for non-contact flora transplantation as well as a construction method and application of the leak-free core-shell hydrogel microsphere delivery system. According to the invention, bacteria are encapsulated in a core of a core-shell microsphere through a two-step photo-initiation emulsion polymerization method of methacrylic acid gelatin, and a non-contact microbiota transplantation system (NMTS) is constructed. The NMTS can be used for biological encapsulation of various strains, shows encapsulation efficiency close to 100%, and has little influence on bacterial activity at the same time. NMTS demonstrates: a shielding effect that protects sealed bacteria from low pH values in the gastrointestinal tract and bile acid disruption; sufficient mechanical strength ensures that bacterial leakage is prevented and the infection risk is eliminated in the whole gastrointestinal tract delivery process; and internal and external substance exchange is realized, and the intestinal microorganism structure is remodeled. The invention provides a unique bacterial cell microencapsulation method which is a non-contact, safe and effective micropopulation transplantation method.

The invention belongs to the technical field of microorganisms, and particularly relates to a complex microbial inoculant for promoting lignocellulose degradation and humus generation in the mulberry twig cow dung composting process and application of the complex microbial inoculant. The complex microbial inoculant is formed by mixing a bacterial strain B1, a bacterial strain F1 and a bacterial strain F2, and the ratio of effective viable counts in the bacterial strain B1, the bacterial strain F1 and the bacterial strain F2 is 1: 1: 1; wherein the preservation number of the bacterial strain B1 is GDMCC No: 65049; the preservation number of the strain F1 is GDMCC No: 65050; the preservation number of the bacterial strain F2 is GDMCC No: 65051. The bacillus cereus B1, the phanerochaete chrysosporium F1 and the smokeless fungi F2 in the microbial composite inoculant are effective microorganisms for promoting the decay of the fertilizer, and the three microorganisms can cooperate with one another in the aerobic composting process, so that the conversion and degradation of raw material substances are fully realized, and a high-quality organic fertilizer is produced. In addition, the composite microbial inoculant can promote the degradation of lignocellulose and the generation of humus in the composting process.

Disclosed are a cement-based battery and a method for manufacturing thereof. The cement-based battery includes a waterproof structure, a battery body, a positive electrode, a negative electrode, and an electrolyte solution. The waterproof structure is provided with an accommodating cavity. The battery body is disposed in the accommodating cavity, and includes a cement-based body, which is obtained by curing a solid-liquid mixture, wherein the solid-liquid mixture includes cement, a first porous material, and a first effective microorganism aqueous solution. The positive electrode and the negative electrode are connected to the battery body respectively and extend out of the waterproof structure. The electrolyte solution is disposed in the accommodating cavity. Therefore, the cement-based battery can be applied to a cement building as an energy storage battery to provide power at night, during power outages or during emergencies.

The present invention belongs to the field of microbial technology and specifically relates to a composite microbial agent for promoting lignocellulose degradation and humus production during mulberry twig and cow dung composting, and its application. The composite microbial agent comprises a mixture of strains B1, F1, and F2, wherein the ratio of effective viable bacteria among strains B1, F1, and F2 is 1:1:1. The deposit number of strain B1 is GDMCC No. 65049; the deposit number of strain F1 is GDMCC No. 65050; and the deposit number of strain F2 is GDMCC No. 65051. The Bacillus cereus B1, Phanerochaete chrysosporium F1, and Sphaerotheca fusca F2 in the composite microbial agent are all effective fertilizer compost-promoting microorganisms. These three microorganisms can synergize with each other during aerobic composting, fully achieving the conversion and degradation of raw materials and producing high-quality organic fertilizer. Furthermore, the composite microbial agent can promote lignocellulose degradation and humus production during composting.

The application belongs to the technical field of fertilizers, and particularly relates to a yeast-source biological organic fertilizer suitable for aquaculture and a preparation method and application thereof. The yeast-source biological organic fertilizer suitable for aquaculture comprises the following components in parts by weight: 40-80 parts of yeast metabolites, 15-30 parts of ammonium chloride, 10-20 parts of monoammonium phosphate and 3-8 parts of a compound microbial agent. The yeast-source biological organic fertilizer suitable for aquaculture has reasonable proportioning, a high number of beneficial bacteria, a good symbiotic environment of multiple bacteria and strong biological activity. The organic active nutrients, effective microbial groups and inorganic nutrients are complete and scientifically proportioned, and the fertilizer has good effects of fertilization, water culture and algae cultivation, can improve the aquaculture water environment, supplement feed organisms, promote the growth of aquaculture objects, improve product benefits and is more conducive to environmental protection.

The invention provides an industrial hemp plant hydroponic nutrient solution and application thereof, and belongs to the technical field of plant cultivation. Comprising the following raw materials in parts by weight: 8 to 12 parts of EM (Effective Microorganisms) fermentation liquor, 1 to 3 parts of compound amino acid, 30 to 40 parts of Hoagland nutrient solution, 4 to 6 parts of humic acid, 0.04 to 0.07 part of chitosan, 0.01 to 0.03 part of chlorogenic acid and 0.01 to 0.03 part of ferulic acid. The plant hydroponic nutrient solution provided by the invention can promote rooting and stem and leaf growth of cannabis sativa, and can also increase the content of effective components. Compared with an existing Hoagland nutrient solution, the plant hydroponic nutrient solution provided by the invention has a more obvious growth promoting effect on cannabis sativa.

The invention belongs to the technical field of biological sewage treatment and energy resource utilization, and particularly relates to a single pure photosynthetic bacterium granulation method with ferroferric oxide nanoparticles as a carrier. According to the method, the pure photosynthetic bacteria are granulated and clustered by taking the ferroferric oxide nanoparticles as the carrier and combining the hydraulic condition, and the granulated photosynthetic bacteria can be effectively separated from liquid in the reactor, so that the content of effective microorganisms in the sewage treatment reactor and the system stability are improved, and the sewage treatment and hydrogen production efficiency is further improved.

The invention discloses a special bio-organic fertilizer for blueberries and a preparation method of the special bio-organic fertilizer, and belongs to the technical field of bio-organic fertilizers. The biological organic fertilizer is prepared from turfy soil, a synergist, pyroligneous liquor, furfural residues, corn straw, phosphoric acid, a modifier, a microbial agent, ferrous sulfate, pine needle leaves, pig manure and EM bacterial liquid. The bio-organic fertilizer is composed of two parts, namely, bio-organic fertilizer particles special for blueberries and a solution. The special bio-organic fertilizer solution for the blueberries is synergized through sodium lignosulphonate and potassium humate with the mass ratio of 1: 1, and the acid soil environment around the roots is improved; activated biochar in the special bio-organic fertilizer particles for blueberries is modified by tea polyphenol and sodium alpha-olefin sulfonate in a mass ratio of 1: 1 and then is grafted with a strong acidic phosphate radical functional group, so that the water retention capacity is improved, and the activated biochar and various substances such as a microbial agent, fermented organic nutrients, turfy soil and ferrous ions play a synergistic role; the blueberry planting soil is improved.

The invention discloses a photovoltaic pond river crab culture method, which comprises the following steps: a bacteria-algae compound conditioning agent is put into a target photovoltaic pond according to a preset period and a preset putting dosage, the bacteria-algae compound conditioning agent comprises an effective microbial flora microbial agent and a chlorella complex which are mixed according to a preset proportion, the pH value of the effective microbial flora microbial agent is 3.0-4.0, the pH value of the chlorella complex is 3.0-4.0, and the pH value of the chlorella complex is 3.0-4.0. The content of lactic acid bacteria in the effective microbial agent is 1.0 * 10 to 1.0 * 10 CFU / mL, the content of saccharomycetes in the effective microbial agent is 1.0 * 10 to 1.0 * 10 CFU / mL, the content of photosynthetic bacteria in the effective microbial agent is 1.0 * 10 to 2.0 * 10 < 3 > CFU / mL, the content of actinomycetes in the effective microbial agent is 1.0 * 10 < 3 > to 3.0 * 10 < 3 > CFU / mL, and the concentration of chlorella in the chlorella complex is 1.0 * 10 cells / mL; and monitoring and dynamically adjusting the water quality in the target photovoltaic pond, and adjusting the putting frequency and the putting dosage of the bacteria-algae compound regulator within a preset range according to a monitoring result so as to maintain or restore the ecological balance of the water body and the river crabs. According to the technical scheme, the problem of micro-ecological unbalance of photovoltaic shading is solved, oxygen is supplied by algae, diseases are inhibited by bacteria, and carbon sequestration by bacteria and algae is realized, so that the breeding survival rate is increased, and the growth of river crabs is promoted.

The invention belongs to the technical field of planting, and particularly relates to a high-position grafting method for an old litchi tree, the method is suitable for being carried out in March to May or September to October every year, and the method specifically comprises the steps of stock treatment, scion treatment, grafting operation and management after grafting, the old litchi tree with the tree age of 10-30 years is selected as the stock, a semi-lignified current-year branch is selected as the scion, and the scion is selected as a semi-lignified current-year branch; a high-position grafting method is adopted for grafting, based on the toughness of semi-lignified current-year branches and control over the scion length, it is ensured that scions and stocks are tightly combined, the risk of fracture caused by external force is reduced, healing of the scions and the stocks is effectively promoted in the mode of section trimming, disinfection treatment and EM bacterial liquid spraying, and it is verified that the scions and the stocks are not prone to fracture. The grafting survival rate reaches 92% or above, fruits can be borne in 2-3 years, high yield can be recovered in 3-4 years, the average plant yield reaches 30 kg or above, and the economic life of old trees can be effectively prolonged.

The application of Microbispora longispora in simultaneously enhancing the lodging resistance and tillering ability of rice belongs to the field of biological bacterial manure and rice planting technology. In order to solve the technical problems that the existing technology lacks an effective microbial technical solution capable of simultaneously solving the requirements of'strong stem' and 'tillering' of rice, the seed soaking and inoculation method cannot be compatible with the conventional seed treatment of bactericidal agents, the effective period is short, and the application effect of Microbispora in actual agricultural production is seriously restricted, the application of Microbispora longispora in simultaneously enhancing the lodging resistance and tillering ability of rice is provided, and specifically, the rice seedbed soil is treated by Microbispora longispora, more contact opportunities and time are given to Microbispora and rice roots, the conventional seed bactericide can be compatible, the lodging resistance and tillering ability of rice can be simultaneously enhanced, and the yield of rice is improved.

The invention relates to the technical field of microbial regulation and control, and particularly discloses a saline-alkali soil improvement method based on microbial regulation and control, which comprises the following steps: S1, screening: screening microorganisms with initial tolerance to saline-alkali environment from natural environment; s2, gene editing: carrying out gene modification on the screened microorganisms by utilizing a CRISPR-Cas9 gene editing system; according to the invention, a CRISPR-Cas9 gene editing system is utilized to carry out gene modification on the screened microorganisms, and the viability of the microorganisms in a saline-alkaline environment is remarkably improved by modifying osmotic pressure regulating genes of the microorganisms, so that the microorganisms can survive and play a role in a more severe saline-alkaline environment, and meanwhile, the survival rate of the microorganisms in the saline-alkaline environment is improved. Plant growth hormone related genes generated by microorganisms are introduced, plant growth is promoted, powerful support is provided for yield increase of saline-alkali soil crops, the genetic characteristics of the microorganisms are accurately regulated and controlled in the gene editing step, the function of the microorganisms is enhanced, and more effective microorganism resources are provided for saline-alkali soil improvement.

The present disclosure relates to compositions and related apparatuses that provide effective microbial inhibiting headspace concentrations of allyl isothiocyanate, citral, thymol, linalool, terpineol, eugenol, or a combination of any two or more thereof.

The invention relates to the field of insect prevention and control, in particular to a high-stress-resistance beauveria bassiana strain CIPPBb075 with high pathogenicity to grassland caterpillars and application of the high-stress-resistance beauveria bassiana strain CIPPBb075. According to the invention, diseased insects parasitized by beauveria bassiana are collected from a field, and the strain is separated and purified, so that the CIPPBb075 strain has relatively high toxicity to grassland caterpillar; the germination rate is increased along with temperature rise, and the spore germination rate is obviously higher than that of other strains; the ultraviolet resistance at 25 DEG C is high; the extracellular protease activity is high; the activity of extracellular chitinase is high. Therefore, the CIPPBb075 strain has relatively strong stress resistance, and can be used as an effective microbial agent for preventing and treating the larvae of the grassland caterpillar compared with other strains.

The present application relates to the field of insect control, in particular to a strong stress-resistant Beauveria bassiana strain CIPPBb075 with high pathogenicity to lema frisoni and its application. The present application is collected from the field by the diseased insects parasitized by the Beauveria bassiana, and the strain is separated and purified, the CIPPBb075 strain has high virulence to lema frisoni; the germination rate increases with the increase of temperature, and the spore germination rate is significantly higher than that of other strains; the ultraviolet resistance is high at 25 DEG C; the extracellular protease activity is high; the extracellular chitinase activity is high. Therefore, the CIPPBb075 strain has strong stress resistance and other strains, and can be used as an effective microbial source drug for controlling lema frisoni larvae.

The invention relates to application of yellow microspora to simultaneous enhancement of lodging resistance and tillering resistance of rice, and belongs to the technical field of biological bacterial fertilizers and rice planting. The technical problems that in the prior art, an effective microbial technical scheme capable of integrally meeting the requirements of'stalk strengthening 'and'tiller increasing' of rice is lacked, a seed soaking and inoculating method cannot be compatible with a conventional bactericide seed treatment agriculture technology, the effective action period is short, and the application effect of yellow microspora fungi in actual agricultural production is seriously restricted are solved. The invention provides application of yellow microspora to simultaneously enhancing lodging resistance and tillering resistance of rice, and particularly relates to application of yellow microspora to treating rice seedbed soil by utilizing yellow microspora, providing more opportunities and time for contact between yellow microspora and rice roots, being compatible with conventional seed bactericides, and being beneficial to improving the lodging resistance and tillering resistance of rice. And the lodging resistance and tillering resistance of the rice can be synchronously enhanced, so that the yield of the rice is increased.

The present disclosure provides a preparation method for a seedling substrate using residues from Populus forests as raw materials and an application. The preparation method for a seedling substrate includes the following steps: crushing Populus fallen leaves into Populus fallen leaf scraps; crushing Populus dead branches into Populus dead branch scraps; mixing the Populus fallen leaf scraps, the Populus dead branch scraps, chicken manures and garden soil to obtain a mixture; adding effective microorganisms (EM) bacterial powder to the mixture for fermentation; and adding perlite during the fermentation to obtain a seedling substrate. The seedling substrate provided in the present disclosure is rich in microorganisms and comprehensive in nutrients, does not require additional fertilizers in a seedling stage, and is capable of promoting the growth of forest seedlings.

Methods and systems are provided for design, improvement, optimization, or personalization of microbiome therapeutics for various diseases. In one embodiment, a method comprises identifying a plurality of microbiome features associated with a disease, building a plurality of simulations by modeling the interactions between a plurality of microbial consortia with a plurality of microbiome samples; predicting one or more microbial consortia that improve the plurality of microbiome features associated with a disease from the plurality of simulations; and optimizing composition of one or more microbial consortia that further improve the plurality of microbiome features associated with a disease from the plurality of simulations or personalizing composition of a microbial consortia according to a patient's baseline microbiome to improve the plurality of microbiome features associated with a disease. In this way, effective microbial consortia for a variety of diseases may be reliably predicted in a high-throughput fashion.

To provide a novel agricultural pellet containing aerobic effective microorganisms and its applications.SOLUTION: One form of this invention is an agricultural pellet containing aerobic effective microorganisms and plant fibers.SELECTED DRAWING: None

The invention provides a compound microbial agent for degrading chemical oxygen demand and a preparation method thereof. The compound microbial agent comprises an effective microbial flora, a carrier and a protective agent, wherein the effective microbial flora is a mixture of at least two of bacillus subtilis, saccharomyces cerevisiae and pseudomonas putida; the carrier is a mixture of bentonite and bran; the protective agent is a mixture of trehalose and skim milk powder. The composite microbial agent can adapt to different application scenes such as water and soil, secondary pollution is avoided in the degradation process, the composite microbial agent has practicability and environmental friendliness, and the production cost and the COD removal cost can be effectively reduced by integrally adopting the raw materials which are low in cost and easy to obtain.

The invention discloses a novel EM (effective microbiota) stock solution and a use method thereof. The stock solution consists of a photosynthetic bacteria group, a lactic acid bacteria group, a saccharomycetes group, an actinomycetes group, a filamentous bacteria group of a fermentation system, bifidobacteria and bacillus. The method can be applied to the fields of cultivation, aquatic products, planting, environmental protection and the like; the organic fertilizer has the characteristics of preventing diseases, promoting growth of animals and plants, improving vitality, shortening the growth cycle and improving the yield in agriculture, and has the effects of inhibiting bacteria, deodorizing and improving the soil environment in the fields of environmental protection and environment; effective strains of the EM stock solution have extremely high deodorization and decomposition capabilities, deodorization work can be completed within 20 seconds, and decomposition work of harmful microorganisms and harmful substances can be achieved within 3 minutes; moreover, the environment resistance is extremely high, 95% of activity (30 minutes) can be kept at the high temperature of 115 DEG C, and the activity is still enough at the low temperature of-27 DEG C.

This invention relates to a layered biofilm-based method for the resource recovery of granular sludge, comprising the following steps: preparing a core of construction waste and a fiber material; uniformly spraying EM (Effective Microorganisms) solution onto the surface of the core of the construction waste; placing the EM-sprayed core of the construction waste into a sludge tank for biofilm formation; then rolling the core of the construction waste onto the fiber material to ensure that the surface of the core of the construction waste after biofilm formation is fully coated with the fiber material; spraying the core of the construction waste with EM solution again and placing it back into the sludge tank for biofilm formation; repeating the steps of spraying EM solution, biofilm formation, and coating with fiber material multiple times; and finally drying. This invention is the first to use a layered biofilm-based, dewatering-free method to granulate sludge, solving the problems of high energy consumption, complex equipment and processes, serious environmental pollution, and difficult treatment associated with traditional sludge thermal drying and other dewatering treatments.

The invention belongs to the field of microorganisms, and particularly relates to application of a microorganism in preparation of a medicine for preventing and / or treating gout and / or hyperuricemia, a medicine composition containing the microorganism and a preparation method of the medicine composition. According to the application of the microorganism in preparing the medicine for preventing and / or treating gout and / or hyperuricemia, an exogenous YbdK gene and / or a coding gene for coding an exogenous YbdK protein are / is inserted into the microorganism; the YbdK gene expresses the exogenous YbdK protein in the microorganism, and / or the exogenous YbdK protein is expressed in the microorganism, the YbdK gene has a nucleotide sequence as shown in SEQ ID NO: 1, and the YbdK protein has an amino acid sequence as shown in SEQ ID NO: 2. The embodiment of the invention provides the application of the stable, safe and effective microorganism in preparation of the medicine for preventing and / or treating gout and / or hyperuricemia so as to meet the clinical treatment requirements.

The present invention belongs to the technical fields of wastewater biological treatment and energy resource utilization, and specifically relates to a method for granulating a single pure strain of photosynthetic bacteria using ferroferric oxide nanoparticles as a carrier. By using ferroferric oxide nanoparticles as a carrier and combining hydraulic conditions to granulate the pure strain of photosynthetic bacteria into agglomerates, the granulated photosynthetic bacteria can be effectively separated from the liquid in the reactor, increasing the content of effective microorganisms in the wastewater treatment reactor and the stability of the system, thereby improving the efficiency of wastewater treatment and hydrogen production.

The invention discloses a soil conditioner for pepper planting. The soil conditioner is prepared from the following components in parts by weight: 6 to 8 parts of continuous cropping soil, 1.5 to 2.5 parts of attapulgite, 0.5 to 1.5 parts of bentonite, 0.2 to 0.5 part of nano zinc oxide, 1 to 2 parts of chitosan oligosaccharide, 0.8 to 1.2 parts of EM (Effective Microorganisms) microbial inoculum and 0.1 to 0.3 part of graphene oxide. The composite application of the nano material and biomacromolecules is introduced into the soil conditioner, and the problems of agglomeration, weak antibacterial ability and the like are solved.

The present disclosure relates to the technical field of ecological water treatment, and particularly relates to a freshwater algae bloom regulation method based on food web construction. The regulation method comprises: performing algae phase regulation, adding effective microbial groups, functional bacterial species and beneficial algae to a target water body, converting harmful algae phases into beneficial algae phases; laying out shellfish in the target water body after the algae phase regulation is completed, purifying the water body through the shellfish, and removing the shellfish before they die; and reconstructing a submerged plant community and releasing a functional fish community in the purified target water body, so as to perfect the structure of the aquatic food web.

PROBLEM TO BE SOLVED: To solve the following problem: when waste seasoning is used in agricultural application for soil improvement and promotion of cultivation of vegetables, fruits and the like, using the waste seasoning as it is or after dilution does not provide soil improvement and a cultivation-promoting effect for vegetables, fruits and the like and, under certain circumstances, causes inhibition of cultivation; SOLUTION: In a seasoning waste treatment liquid and its production method, effective microorganisms essentially containing bacteria and fungi is added to an aqueous solution of the waste seasoning to culture the mixture under an anaerobic environment;

This invention relates to the field of microbial technology, specifically disclosing the application of a recombinant microorganism and a lactate dehydrogenase mutant in the fermentation production of threonine. The invention discovered that specific mutations in lactate dehydrogenase can enhance the ability of fermenting bacteria to produce threonine, and thus proposes a recombinant microorganism in which the alanine at position 265 of the wild-type lactate dehydrogenase is mutated to threonine or serine; the amino acid sequence of the wild-type lactate dehydrogenase is shown in any one of SEQ ID No. 25-27, and the starting strain of the recombinant microorganism is a Corynebacterium capable of fermenting and producing threonine. This invention provides a new and effective microorganism and method for threonine production.

The present invention discloses a malathion-degrading bacterium derived from tidal flats and its application, which relates to the field of biotechnology. The malathion-degrading bacterium is Ectopseudomonas khazarica FJ5-34-2, which was deposited on November 8, 2024 at the General Microbiology Center of the China National Center for Culture Collection of Microorganisms. The deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No. 32547. The present invention screened a strain of Ectopseudomonas FJ5-34-2 from tidal flat samples in Beidaihe New Area, Qinhuangdao, Hebei Province. This Ectopseudomonas can effectively degrade malathion, and the degradation rate of 100 mg / L malathion can reach 94.93% within 72 h. The present invention provides a new and effective microorganism for the microbial degradation of malathion, which has great practical significance for alleviating the environmental residues of pesticides.