The present invention relates to a Pu'er tea toothpaste and a preparation method thereof. The Pu'er tea toothpaste is characterized by comprising a Pu'er tea extract, which is obtained by steps of low temperature pulverization, ultrasonic extraction, pressure filtration, separation and concentration. The Pu'er tea extract is combined with food-grade auxiliary materials comprising glycerin, sorbitol, PEG400, tetrasodium pyrophosphate, sodium dihydrogen phosphate, sodium phytate, sodium carboxymethyl cellulose, xanthan gum, sodium lauryl sulfate, calcium phosphate dihydrate, hydrated silica and essence to prepare the Pu'er tea toothpaste. The method fundamentally eliminates quality issues such as spoilage, degeneration, color changing, flavor changing and chemical safety of the tea toothpaste, and effectively prolongs a shelf period of the tea toothpaste. The tea toothpaste has multiple special efficacies, such as removing scale, decomposing residual smoke stains, tea stains and coffee stains in slits between the teeth, reducing plaque, preventing breeding of harmful bacteria, disinfecting, diminishing inflammation, stopping bleeding, eliminating odor, promoting the secretion of saliva, moisturizing mouth, recovering taste buds, improving taste sense, and enabling a user to taste better flavor of food, and is a natural healthful tea toothpaste.