371 results about "Saccharomycetes" patented technology

The invention relates to the technical field of animal feed preparation, in particular to a functional feed additive for ruminants and a preparation method of the functional feed additive for the ruminants, and the functional feed additive for the ruminants is characterized by consisting of the following components in percentage by mass: probiotics which are saccharomycetes, bacillus subtilis and enterococcus faecalis; an enzyme preparation, xylanase and protease; prebiotics are added; a microcapsule coating material; a binder; the balance is a carrier; the probiotics and the enzyme preparation are wrapped in the chitosan / sodium alginate composite film through a microcapsule coating technology, adverse factors such as temperature, humidity and illumination in the production, transportation and storage process can be effectively isolated, meanwhile, in the rumen low-pH, strong-protease and complex-microorganism environment, the coating layer can slowly release and protect active ingredients, and the effect of preventing and treating the probiotics and the enzyme preparation is achieved. Effective content and activity during final feeding are ensured, consistent growth promotion can be kept in different varieties, weights and feeding stages, and production benefits and feeding stability are improved.

The preparation method comprises the following steps: mixing saccharomycetes, lactic acid bacteria and bacillus subtilis according to a ratio of 1: 1: 1 to obtain mixed bacteria; 0.25 g of mixed bacteria is taken and dissolved in 5 mL of sterile water for standby application; slicing and crushing the dried rhizoma polygonati, and sieving with a 10-mesh sieve to obtain rhizoma polygonati powder; the preparation method comprises the following steps: mixing water and rhizoma polygonati powder according to a liquid-material ratio of 10mL: 1g, sterilizing at 121 DEG C for 30min, cooling, adding mixed bacteria according to an inoculum size of 3% of the mass of rhizoma polygonati, culturing in a constant-temperature shaking table incubator at 120r. Min <-1 > for 72h at the culture temperature of 035 DEG C, sterilizing at 100 DEG C for 30min after fermentation, cooling and centrifuging to obtain rhizoma polygonati fermentation liquor, and storing at 4 DEG C. The prepared polygonatum sibiricum fermentation liquor is high in active ingredient content, has good cell safety, anti-oxidation, anti-inflammatory and whitening effects, and has the potential of being used for developing functional raw materials of traditional Chinese medicine cosmetics.

The invention relates to the technical field of daily chemicals, in particular to multi-fermented oil and application thereof. The multi-fermented oil is prepared from Tibetan cordyceps sinensis, eurotium cristatum and saccharomycetes. The multi-fermented oil provided by the invention contains functional components and mild components, and contains rich fatty acids, antioxidant components and other substances with biological activity. A lipid structure in the fermented oil is similar to a natural sebum layer of skin, so that a protective barrier can be formed on the surface of the skin, and water loss is prevented. This is particularly beneficial to dry skin and helps the skin maintain moist and soft. And micromolecular lipid components in the fermented oil, such as unsaturated fatty acid, can more easily permeate into the deep layer of the skin, so that long-acting nourishing and moisturizing effects are provided for skin cells.

The invention relates to the technical field of feeds, and particularly discloses a cattle fattening feed which is technically characterized by comprising the following components in percentage by weight: 20-60 parts of a fermented mixture, 10-16 parts of modified coconut fiber, 0.5-1.5 parts of an additive and 5-10 parts of an additive, the fermented material mixture is prepared from soybean meal, corn, rice bran, pennisetum purpureum, hops, an aspergillus oryzae culture, saccharomycetes, bacillus subtilis and bacillus amyloliquefaciens; the mass ratio of the soybean meal to the corn to the rice bran to the pennisetum purpureum to the hops is (2-4): (1-3): (3-5): (2-4): (0.4-0.9); the additive is fructus momordicae residues; the additive is a mixture of codonopsis pilosula and rhizoma atractylodis. The feed disclosed by the invention contains the siraitia grosvenorii residues, so that the apparent digestibility, the growth performance, the serum biochemical index and the antioxidant index of the fattening cattle can be improved, and the quality of the fattening cattle can be effectively improved.

The invention relates to the technical field of saline-alkali soil improvement, and discloses a composite improved saline-alkali soil composition based on biomass charcoal and a preparation method thereof.Biomass charcoal, glucose, lactic acid bacteria, saccharomycetes, aluminum sulfate and a quaternary ammonium salt modifier are added into a stirrer to be stirred and mixed, stirring is conducted, and a mixture is obtained; and obtaining the composite improved saline-alkali soil composition based on the biomass charcoal. A quaternary ammonium salt group in the quaternary ammonium salt modifier can reduce the pH value of the soil and reduce the alkalinity of the soil, so that the salinization condition of the soil is improved, and quaternary ammonium salt can promote the activity of soil microorganisms and improve the fertility of the soil and the growth environment of crops; the siloxane is converted to be neutral by adjusting the pH value of the soil, the siloxane is beneficial to reducing the alkalinity of the soil, and a more suitable growth environment is provided for plants; wherein the contained boric acid group reduces the pH value of the soil by neutralizing alkaline substances in the soil, and has a relatively good improvement effect on the saline-alkali soil.

The invention relates to the technical field of food processing, in particular to cubilose nourishing, conditioning and blood sugar reducing health-care food and a preparation process thereof. The cubilose enters the palace from the clear generation, becomes a defending tonic, is a medicinal and edible tonic, and has the beauty of a rare famous drug and an oriental treasure. The cubilose is rich in sialic acid, protein, amino acid and other bioactive substances, so that the cubilose has a nourishing effect and has certain health-care and conditioning effects on the lung, the kidney and the stomach. According to the preparation method, the cherry and the honeysuckle are subjected to synergistic fermentation through the rhamnolactobacilli and the saccharomycetes, so that the performance is improved, and the prepared cubilose health-care food has the effect of reducing blood sugar.

The invention discloses hansenula polymorpha with low ethanol yield and high aroma yield and application of hansenula polymorpha, and belongs to the technical field of microorganisms. According to the invention, a strain of high-aroma-yield saccharomycetes, which is hanseniaspora uvarium, is separated from fruits, and the preservation number of the saccharomycetes is CCTCC (China Center for Type Culture Collection) NO: M 20242562. The quality of the low-alcohol yellow peach beverage fermented by the high-aroma-yield strain provided by the invention is improved, and the alcoholic strength after fermentation is well reduced and is only 0.4% vol. Through flavor substance detection before and after fermentation, flavor substances generated by the strain are rich in variety, for example, 90.73 mu g / kg of phenethyl alcohol is newly generated. Sensory evaluation shows that the low-alcohol yellow peach beverage fermented by the strain is outstanding in flower fragrance and fruit fragrance, the richness and the balance degree are improved, it is proved that the overall flavor characteristics of the low-alcohol beverage can be remarkably improved, and more pleasant sensory experience is brought to consumers.

The invention discloses a preparation technology of directional debitterizing yellow tea, and belongs to the technical field of tea processing. Fresh tea leaves are used as raw materials, the yellow tea is prepared through the processes of pretreatment, fermentation, fixation, rolling, heaping for yellowing and drying, during fermentation, a compound bacteria solution and the spread and aired fresh leaves are mixed and fermented, strains in the compound bacteria solution comprise lactic acid bacteria, saccharomycetes and bacillus, and through the synergistic effect of the lactic acid bacteria, the saccharomycetes and the bacillus, the yellow tea is prepared. Balancing of directional degradation of bitter and astringent substances and generation of flavor substances is achieved, high-quality yellow tea is prepared through efficient debitterizing, the stage heaping yellowing technology is adopted, primary heaping and secondary heaping are adopted for two-stage heaping yellowing, the quality of the yellow tea is enhanced, and poor taste caused by excessive heaping yellowing is avoided. According to the finished tea prepared by the preparation process, the content of tea polyphenol is reduced to 18%-20% through detection, the content of amino acid reaches 4.5%-5.0%, bitter taste and astringent taste are relieved, sweet taste is added, and the taste is mellow.

The invention provides a preparation method of Keemun camellia seed fermented oil, and belongs to the technical field of vegetable oil production. The preparation method comprises the following steps: S1, preparing recombinant saccharomycetes; s2, jointly fermenting the antarctic torulopsis, the recombinant saccharomycetes and the Keemun camellia seed oil; s3, separating an oil phase from a water phase, and taking the oil phase; and S4, dehydrating the oil phase to obtain the Keemun camellia seed fermented oil. Preferably, according to the preparation method of the recombinant saccharomycetes in the step S1, phenylalanine deaminase, cinnamate hydroxylase, 4-coumaric acid CoA ligase, chalcone synthetase, chalcone reductase and peroxidase are expressed in saccharomyces cerevisiae. According to the Keemun camellia seed fermented oil prepared by the method disclosed by the invention, the characteristic components of the Keemun camellia seed oil can be remarkably improved, the antioxidant capacity of the Keemun camellia seed oil is enhanced while the skin feeling of the Keemun camellia seed oil is improved, and the application value of the Keemun camellia seed oil in cosmetics is improved.

The invention relates to the technical field of daily chemicals, in particular to a dark tea extract containing eurotium cristatum composite strains for solid aerobic fermentation. The composite strains comprise eurotium cristatum and saccharomycetes. The dark tea extract provided by the invention is stable in performance and not easy to discolor, contains components such as polysaccharide, amino acid and mineral substances, and can help skin moisturize. The permeability of the components is enhanced in the fermentation process, moisture can be locked on the surface layer of the skin, and the moisture degree of the skin is kept. The skin care product has very strong antioxidant capacity, and can neutralize free radicals, slow down the oxidation process of skin, delay skin aging and reduce wrinkles and fine wrinkles. A certain calming effect can be achieved on the skin which is prone to redness and itching or has slight inflammation.

The present invention relates to the technical field of feeds and particularly relates to a piglet weaning transition feed containing lactic acid bacteria and saccharomycetes compound fermented soybean meal and a preparation method thereof, and the piglet weaning transition feed comprises the following components in parts by weight: 15-20 parts of compound fermented soybean meal, 45-50 parts of corn, 10-15 parts of wheat, 5-7 parts of fish meal, 3-5 parts of whey powder, 3-4 parts of vegetable oil, 1-2 parts of an amino acid supplement and 2-3 parts of a mineral premix, 1-2 parts of a vitamin premix and 1.5-2 parts of a calcium-phosphorus supplement; wherein the compound fermented soybean meal is prepared by performing compound fermentation on soybean meal through lactic acid bacteria and saccharomycetes, and a complementary and symbiotic microbial ecological system is formed by carefully selecting and optimizing the combination proportion of three lactic acid bacteria and two saccharomycetes, so that the fermentation efficiency and stability are improved, and the functionality of a fermentation product is enhanced. Different strains complement each other in the aspects of carbohydrate metabolism, pH tolerance and functional characteristics, and nutrition competition is reduced.

The invention belongs to the technical field of microbial protein preparation, and particularly relates to a method for preparing microbial protein from crop straw and application of the microbial protein. According to the method, the crop straw is subjected to biomass conversion pretreatment, so that the accessibility and hydrolysis efficiency of cellulose are improved; on the basis, the pretreated biomass is subjected to enzymolysis through cellulase, cellulose and hemicellulose components in the biomass are converted into glucose and xylose, and the glucose and the xylose are utilized by saccharomycetes in the subsequent saccharomycetes fermentation process to synthesize the microbial protein. The microbial protein obtained through the method has the advantages of being rich in nutrition and high in essential amino acid content, and has wide application prospects in the fields of food and feed. Therefore, after the crop straw is subjected to chemical or physical-biological treatment, a new way is provided for high-value utilization of the crop straw on the basis of obtaining the nutrient-rich microbial protein.

The invention provides an application of lactobacillus mucus B14.32 in traditional Chinese medicine fermentation and an anti-depression traditional Chinese medicine fermentation composition, a microbial fermentation technology is combined with traditional Chinese medicine extraction, and lactobacillus mucus B14.32 and saccharomycetes are used for carrying out two-step fermentation on traditional Chinese medicine with an anti-depression effect to obtain the anti-depression traditional Chinese medicine fermentation composition. The active ingredients of the traditional Chinese medicines are preserved to the maximum extent, the medicine effect is enhanced, the toxicity is reduced, the obtained anti-depression traditional Chinese medicine fermentation composition has the effects of resisting depression and relieving brain inflammation of depression patients, and a safer and more effective treatment scheme is provided for the depression patients.

The invention discloses a method for converting livestock and poultry manure into an organic fertilizer through microbial fermentation, and belongs to the technical field of livestock and poultry manure conversion. The method comprises the steps that firstly, the livestock and poultry manure is subjected to physical sorting, lime or monopotassium phosphate is added according to the pH value detection result, and the pH value is adjusted to 6.5-7.5; after determining nutrient components of the feces, adding soluble salt trace elements such as zinc sulfate and mixed carbohydrates of cane sugar and glucose; traditional strains of bacillus, lactic acid bacteria, saccharomycetes and actinomycetes are mixed with novel strains separated from the periphery of a livestock and poultry farm, optimized culture is conducted through a two-step culture method, and plant extracts such as lily extracts or livestock and poultry intestinal metabolites such as short-chain fatty acid are added in the culture process. The fermentation process adopts a rotatable fermentation tank body, and comprises three stages of fermentation of rapid heating, high-temperature stabilization and cooling decomposition. And finally, low-temperature vacuum drying, crushing, granulation and addition of a coating material are performed to prepare the organic fertilizer, so that efficient resource utilization of the livestock and poultry manure is realized.

The invention belongs to the technical field of skin care, and discloses a soothing and moisturizing composition, a preparation method, application and a skincare product, the soothing and moisturizing composition is obtained by taking jujube fruits, Chinese wolfberry fruits and centella as fermentation substrates and fermenting saccharomycetes and / or galactose yeast-like bacteria in the fermentation substrates, the weight ratio of the jujube fruits to the Chinese wolfberry fruits to the centella asiatica is (1-5): (2-8): (1-2). The soothing and moisturizing composition obtained by combined fermentation of the jujube fruits, the Chinese wolfberry fruits and the centella asiatica as fermentation substrates can generate a synergistic effect, so that the skin soothing, moisturizing and antioxidant effects of the soothing and moisturizing composition are improved.

The invention relates to the technical field of microbial fermentation feeds, in particular to a method for producing a protein feed through solid-state fermentation of distiller's grains based on Trichoderma harzianum pretreatment. According to the method, the vinasse is pretreated by the crude enzyme prepared from trichoderma harzianum, and the formula and fermentation conditions of an enzyme production culture medium are optimized, so that the filter paper enzyme activity of cellulase is remarkably improved. By adding the surfactant into a fermentation system and optimizing the variety of the surfactant, the yield of the fermented monosaccharide is improved, and the problem of musty taste in a traditional fermented product is solved while the degradation efficiency of lignocellulose is improved. Furthermore, a synchronous saccharification and fermentation process is adopted, vinasse is fermented through compounded saccharomycetes and crude enzyme liquid, the trichoderma harzianum fermented protein feed is prepared and obtained, digestion and absorption of animals are easier, lactic acid fermentation is conducted on the trichoderma harzianum fermented protein feed again through lactobacillus fermentum, the flavor of the feed is improved, the bitter taste of the feed is reduced, and the sour taste is improved. The method realizes efficient utilization of vinasse resources.

The invention provides a soilless substrate for blueberry cultivation and a preparation method thereof, and relates to the technical field of soilless culture of blueberries. The soilless substrate is prepared from turf, vermiculite, sawdust, pea vine treatment powder, modified coco coir, a walnut green seedcase fermented material, manure, calcium propionate and maltooligosaccharide, and the pea vine treatment powder is prepared by extracting pea vines with ethanol, boiling and drying; the walnut green seedcase fermented material is prepared by fermenting walnut green seedcases with saccharomycetes, sterilizing and fermenting with lactobacillus plantarum. According to the method, the defects in the prior art are overcome, the growth efficiency of blueberry soilless culture is improved, the high yield of blueberries is promoted, and the economic benefits in soilless culture are improved.

The invention discloses an ecological planting method for remarkably improving the amino acid content of citrus, which comprises the following steps: (1) mixing and fermenting pruned citrus branches, weed straws, livestock and poultry manure and indigenous probiotic ferment powder to prepare a basic microbial fertilizer, and adding a calcium magnesium phosphate fertilizer and monopotassium phosphate to prepare a special microbial fertilizer for citrus, applying as a base fertilizer in winter; (2) after citrus pruning, in key phenological periods such as a flower bud period, a young fruit period and a fruit swelling period, an indigenous probiotic enzyme diluent with a specific dilution ratio is used for systematic foliage spraying on a tree body. The indigenous probiotic enzyme contains bacillus amyloliquefaciens, saccharomycetes, active enzymes and a plurality of nutrient elements. The method can significantly increase the total amino acid content of citrus fruits, greatly increase the yield, improve the taste of the fruits, enhance the tree body resistance, reduce the use of chemical pesticides, improve soil, and realize quality improvement, yield increase and green sustainable development of citrus planting.

The invention discloses a novel microbial sludge reduction inoculant and a preparation method and application thereof, and belongs to the technical field of microorganisms, the microbial sludge reduction inoculant comprises a composite inoculant and a biomass additive; the complex microbial inoculant is prepared from the following components in parts by weight: 10 to 12 parts of lactic acid bacteria, 18 to 22 parts of saccharomycetes, 6 to 8 parts of bacillus licheniformis, 12 to 16 parts of bacillus subtilis, 4 to 6 parts of bacillus amyloliquefaciens, 7 to 9 parts of methane sarcina and 3 to 5 parts of horsehair methane bacteria; the biomass additive is fruit peel waste. According to the method disclosed by the invention, a biodegradation system with high activity of various required enzymes can be formed by utilizing the mutual action of microbial flora, dead or aged thalli in the activated sludge can be effectively destroyed and decomposed, meanwhile, organic matters can be decomposed, the sludge is subjected to reduction treatment under the condition that the effluent quality is not influenced, the treatment cost is low, and the treatment effect is good. The method is green and pollution-free and worthy of popularization.

The invention belongs to the technical field of fermentation engineering, and particularly relates to turmeric meal fermented feed as well as a preparation method and application thereof. Iron, magnesium and zinc elements are added into fermentation base materials such as turmeric meal and soybean meal, and the mixture is fermented through a bacterium-enzyme synergistic fermentation inoculant to generate the turmeric meal fermented feed, wherein the fermentation inoculant comprises bacillus subtilis, lactobacillus plantarum and saccharomycetes. The prepared turmeric meal fermented feed can be applied to feed feeding of laying hens or broilers. Results of the embodiment of the invention show that the turmeric meal fermented feed prepared by the invention not only can effectively inhibit harmful bacteria in vitro, but also can remarkably improve the chicken production performance, the eggshell quality and the yolk color, and effectively improve the chicken quality of yellow feather broilers.

The invention provides a preparation method of 11-seed fermentation oil and fermentation liquor with multiple effects, and belongs to the technical field of fermentation. The method comprises the following steps: S1, preparing recombinant saccharomycetes; s2, jointly fermenting lactobacillus, recombinant saccharomycetes and 11 seed oil; s3, separating an oil phase and a water phase, and taking the oil phase; and S4, dehydrating the oil phase to obtain the 11-seed fermentation oil. And S5, further refining the water phase to obtain the 11-seed fermentation liquor. The 11-seed fermented oil prepared by transforming a microbial metabolic network is rich in dihydroquercetin, and has multiple effects of resisting oxidation, resisting wrinkles, tightening, repairing cells and the like. In the fermentation process of the 11-seed fermentation oil, 11-seed fermentation liquor can be obtained, and the fermentation liquor has multiple effects of resisting oxidation, relieving, moisturizing, resisting wrinkles, tightening and the like.

The invention belongs to the field of nano biomedicine, and particularly discloses a yeast bionic vaccine based on a metal organic framework ZIF-8 as well as a construction method and application of the yeast bionic vaccine, and the structure of the bionic vaccine is that ZIF-8 loaded with antigen OVA is encapsulated in a yeast shell. The invention relates to preparation and anti-tumor immune response of the bionic vaccine. The yeast shell is obtained by performing acid and alkali treatment and organic solvent washing on saccharomycetes and has the effects of targeting antigen presenting cells and stimulating immune response, and the antigen-loaded metal organic framework ZIF-8 is encapsulated in the yeast shell and has relatively good stability and an effect of continuously releasing antigens. The ZIF-8 is decomposed in antigen presenting cells, and lysosome escape of the antigen is induced. The yeast bionic vaccine provided by the invention can specifically target antigen presenting cells, continuously release tumor antigens in vivo, mediate escape of lysosome of the antigens and stimulate strong anti-tumor immune response, and has a better application prospect.

The invention belongs to the technical field of microbial fermentation, discloses a method for multi-bacterium synergistic fermentation of roxburgh rose juice, and particularly discloses a microbial agent which comprises rhizopus arrhizus CLF001 and at least one of pediococcus pentosaceus, lactobacillus plantarum, acetic acid bacteria, saccharomycetes and lactobacillus acidophilus. According to the microbial agent provided by the invention, the content of polysaccharide, polyphenol, brass and the like can be remarkably increased and the content of tannin in the roxburgh rose normal juice can be reduced by fermenting the roxburgh rose normal juice for 3 days by using the microbial agent, so that the fermented roxburgh rose normal juice does not feel obvious astringent taste, and the bitterness of the roxburgh rose normal juice is greatly improved; when the microbial agent is used for fermentation, polyphenol substances such as tannin with heavy astringent taste in the roxburgh rose normal juice are converted into non-astringent polyphenol substances, the taste of the normal juice is improved, the content of active ingredients is increased, for example, key ingredients (such as polyphenol, flavone and polysaccharide) capable of remarkably improving the antioxidant activity in the roxburgh rose normal juice can be used for improving the antioxidant performance of the roxburgh rose normal juice.

A peptide composition for improving tear stains of pets belongs to the field of pet health, is used for improving tear stains of pets, and is characterized by comprising 20-30 parts of sea cucumber peptide, 5-10 parts of casein phosphopeptides, 10-20 parts of soybean peptide, 20-30 parts of corn oligopeptide, 10-20 parts of wheat oligopeptide and 10-20 parts of mussel peptide, the amino acid sequence of common mussel peptide is His-Gly-Ala-Asp-Gly-Val-Pro-Pro-Phe, and the amino acid sequence of the common mussel peptide is His-Gly-Ala-Asp-Gly-Val-Pro-Pro-Phe. The amino acid sequence of the perna viridis peptide is Asp-Ser-Ser-Leu-Pro-Pro-Ser-Arg-Pro-Thr, and the perna viridis peptide has the effects of effectively improving the metabolism of ferriporphyrin, inhibiting the activity of saccharomycetes and reducing the formation of tear stains of pets.

The invention discloses a combined preparation for improving rice quality and application thereof, and belongs to the technical field of crop planting. The combined preparation is prepared from the following raw materials in parts by weight: radix angelicae sinensis, radix paeoniae alba, rhizoma atractylodis macrocephalae, fructus forsythiae, oyster mushroom bran, needle mushroom bran, a microbial agent, ammonium nitrate, potassium nitrate, ammonium molybdate, DTA-6, zinc sulfate and monopotassium phosphate. The microbial agent is prepared from bacillus megatherium, pseudomonas putida, serratia marcescens and saccharomycetes. The combined preparation for improving the rice quality is prepared by taking natural Chinese herbal medicines, fungus chaff, microbial strains and a plant growth regulator as raw materials. The combined preparation is rich in polysaccharide, amino acid, chitosan oligosaccharide and other components and active microbial agents, and can promote the growth and development of rice and improve the quality of rice. The combined preparation provided by the invention provides a new technical thought for rice quality improvement.

The invention relates to an oil-control liposome as well as a preparation method and application thereof. The oil-control liposome comprises a liposome carrier and an oil-control composition loaded in the liposome carrier, the oil control composition comprises a yeast / zinc fermentation product, a lotus corniculatus seed extract, a lactobacillus fermentation lysate and VII type collagen. According to the skin oil control composition, four specific active components including the saccharomycetes / zinc fermentation product, the lotus corniculatus seed extract, the lactobacillus fermentation lysate and the VII type collagen are creatively combined, and the four specific active components are mutually matched and have a synergistic effect, so that an excellent skin oil control effect is comprehensively obtained in a multi-channel and multi-dimensional manner; in addition, potential mutual promotion and synergistic interaction effects are achieved on the oil control effect. Furthermore, the oil-control composition promotes permeation of effective components and acts on effective parts through a liposome wrapping technology, grease generation and secretion are controlled in a multi-channel mode, and the effective oil control effect is achieved.

The invention provides a construction method of engineering saccharomycetes for efficient biosynthesis of chelerythrine. Specifically, the invention provides an engineering bacterium for producing chelerythrine, the engineering bacterium is saccharomycetes, and the engineering bacterium contains exogenous and / or endogenous gene modules, such as BBE, SMT, TDC, TNMT, MSH, P6H, CPR and BUP1 gene modules. The invention also provides a construction method, application and a construction kit of the engineering bacterium, and a production method and a fermentation system of chelerythrine. The engineering bacteria can efficiently synthesize chelerythrine, the yield is far higher than that in the prior art, and the highest yield reaches 4.5 mg / L. Therefore, the engineering bacterium and the construction method provided by the invention can overcome the defect of long production period of chelerythrine in the field, and a new method is provided for synthesis of chelerythrine.

The invention belongs to the technical field of biosynthesis and traditional Chinese medicine essence extraction, and particularly relates to a biosynthesis Chinese herbal medicine anti-hair loss pilatory and a preparation method thereof. The biosynthetic Chinese herbal medicine hair loss preventing and hair growing agent comprises the following effective components: a pseudomonas subtilis fermentation extract and a saccharomycetes fermentation extract. The hair-loss-preventing and hair-growing shampoo has the effects of preventing hair loss and growing hair through complementation and synergistic interaction of cleaning pores, inhibiting fungal reproduction, penetrating nutrient components into scalp and the like, activating dermis hair follicles, expanding blood capillaries, removing slough and promoting tissue regeneration, and promoting hair cell division, and has a relatively good application prospect.

The invention discloses an extraction method and application of lavender essential oil based on yeast fermentation pretreatment, and belongs to the technical field of plant active ingredient extraction. The method comprises the following steps: activating saccharomycetes, inoculating the saccharomycetes into a mixture containing a crushed lavender raw material and water, and fermenting at 30-38 DEG C for 20-28 hours to obtain fermentation liquor; then sterilizing and centrifuging the fermentation liquor to obtain supernate; and finally extracting the essential oil through steam distillation. Through yeast fermentation pretreatment, the lavender cell wall structure is effectively destroyed, essential oil release and active ingredient conversion are promoted, and the essential oil yield and antioxidant activity are remarkably improved. Compared with a traditional steam distillation method, the method has the advantages that the essential oil yield can be increased from 0.55% to 0.75% or above, and the DPPH free radical scavenging rate can be increased to 51.75% or above. The method is green in process and simple and convenient to operate, a new way is provided for high-value utilization of lavender resources, and the prepared essential oil can be used for developing cosmetics with anti-oxidation and anti-aging effects.

The embodiment of the invention discloses application of lactobacillus delbrueckii. The application is characterized in that the lactobacillus delbrueckii is applied to inhibition of mesophilic aerobic bacteria, saccharomycetes, mould and coliform bacteria. The lactobacillus delbrueckii has a preservation number of CGMCC No.11247. In the lactobacillus delbrueckii activating process, bacteriocin can be generated and can remarkably inhibit the mesophilic aerobic bacteria, saccharomycetes, mould and coliform bacteria on the surfaces of fruits and vegetables, so that the preservation effect of thefruits and vegetables can be improved. Furthermore, secondary metabolites of the lactobacillus delbrueckii can be biodegraded and digested by human bodies, and are healthy and harmless to human bodies.