52 results about "Saccharomycetes" patented technology

The invention discloses an ecological planting method for remarkably improving the amino acid content of citrus, which comprises the following steps: (1) mixing and fermenting pruned citrus branches, weed straws, livestock and poultry manure and indigenous probiotic ferment powder to prepare a basic microbial fertilizer, and adding a calcium magnesium phosphate fertilizer and monopotassium phosphate to prepare a special microbial fertilizer for citrus, applying as a base fertilizer in winter; (2) after citrus pruning, in key phenological periods such as a flower bud period, a young fruit period and a fruit swelling period, an indigenous probiotic enzyme diluent with a specific dilution ratio is used for systematic foliage spraying on a tree body. The indigenous probiotic enzyme contains bacillus amyloliquefaciens, saccharomycetes, active enzymes and a plurality of nutrient elements. The method can significantly increase the total amino acid content of citrus fruits, greatly increase the yield, improve the taste of the fruits, enhance the tree body resistance, reduce the use of chemical pesticides, improve soil, and realize quality improvement, yield increase and green sustainable development of citrus planting.

The invention discloses an extraction method and application of lavender essential oil based on yeast fermentation pretreatment, and belongs to the technical field of plant active ingredient extraction. The method comprises the following steps: activating saccharomycetes, inoculating the saccharomycetes into a mixture containing a crushed lavender raw material and water, and fermenting at 30-38 DEG C for 20-28 hours to obtain fermentation liquor; then sterilizing and centrifuging the fermentation liquor to obtain supernate; and finally extracting the essential oil through steam distillation. Through yeast fermentation pretreatment, the lavender cell wall structure is effectively destroyed, essential oil release and active ingredient conversion are promoted, and the essential oil yield and antioxidant activity are remarkably improved. Compared with a traditional steam distillation method, the method has the advantages that the essential oil yield can be increased from 0.55% to 0.75% or above, and the DPPH free radical scavenging rate can be increased to 51.75% or above. The method is green in process and simple and convenient to operate, a new way is provided for high-value utilization of lavender resources, and the prepared essential oil can be used for developing cosmetics with anti-oxidation and anti-aging effects.

The embodiment of the invention discloses application of lactobacillus delbrueckii. The application is characterized in that the lactobacillus delbrueckii is applied to inhibition of mesophilic aerobic bacteria, saccharomycetes, mould and coliform bacteria. The lactobacillus delbrueckii has a preservation number of CGMCC No.11247. In the lactobacillus delbrueckii activating process, bacteriocin can be generated and can remarkably inhibit the mesophilic aerobic bacteria, saccharomycetes, mould and coliform bacteria on the surfaces of fruits and vegetables, so that the preservation effect of thefruits and vegetables can be improved. Furthermore, secondary metabolites of the lactobacillus delbrueckii can be biodegraded and digested by human bodies, and are healthy and harmless to human bodies.

The invention discloses a composition containing saccharomycetes / rice fermentation product filtrate, which has the effects of controlling oil and repairing hair. The composition comprises saccharomycetes / rice fermentation product filtrate, a rice husk extract, a tuber melanosporum extract and a millet seed extract. The invention also provides an application of the composition in preparation of oil-controlling, repairing and washing products, shampoo containing the composition and a preparation method of the shampoo. Experimental results show that the composition can significantly inhibit grease secretion of human sebaceous gland cells, repair hair and improve smoothness and glossiness of hair. The composition disclosed by the invention has the advantages of strong synergistic effect of all components, safety, no irritation, simple preparation process and suitability for industrial production.

The invention belongs to the field of new materials for daily use chemicals, and discloses a method for fermenting solanum nigrum, which is characterized in that solanum nigrum roots are fermented by adopting one or more strains of lactic acid bacteria, bifidobacteria, bacillus, micrococcus, saccharomycetes and filamentous fungi to obtain a solanum nigrum fermentation product. The functions of wrinkle resistance, compactness and the like of the withanolaria nipponica fermentation product obtained by fermentation through the method are obviously better than those of unfermented withanolaria nipponica fermentation products and fermentation products taking withanolaria nipponica extracts as fermentation bases. Meanwhile, the invention also provides application of the fermentation product.

The invention belongs to the technical field of biological gene engineering, and particularly relates to escherichia coli with high RNA content and a construction method and application thereof.A strong promoter is used for replacing a promoter of escherichia coli rDNA, an expression element of strong promoter + rDNA + terminator is constructed, plasmid is used as a carrier, the expression element is transferred into escherichia coli, and an escherichia coli engineering strain with high RNA yield is constructed; wherein the rDNA is derived from one or more of rrnA, rrnB, rrnC, rrnD, rrnE, rrnG and rrnH of rDNA operons of escherichia coli, and comprises 16S rDNA, 23S rDNA and 5S rDNA in the corresponding operons. By adopting the construction method, the RNA content of the escherichia coli can be obviously improved, and when the method is used for producing RNA, the RNA yield is obviously higher than that of an initial escherichia coli strain and common saccharomycetes for RNA production at present.

The invention discloses a method for preparing an amino acid fertilizer by multi-strain synergistic starter propagation and a liquid enzymolysis method, which comprises the following steps: selecting 25%-35% of bacillus subtilis, 10%-20% of trichoderma reesei, 15%-25% of aspergillus oryzae, 10%-20% of lactic acid bacteria, 5%-15% of saccharomyces cerevisiae and 5%-15% of aspergillus niger as composite strains; according to the method, six strains such as bacillus subtilis and trichoderma reesei form a composite microbial system, soybean meal and bran are used as raw materials, and the problems of low degradation efficiency, unstable enzyme activity and the like of a traditional process are solved through a process of'three-stage temperature-controlled starter propagation and four-stage liquid fermentation '; the method is characterized in that a'bacillus-mould-saccharomycetes-lactic acid bacteria 'four-flora synergistic system is constructed, a starter propagation-fermentation multi-parameter coupling regulation and control process is initiated, a'process-quality-effect' closed-loop mechanism is established, compared with a traditional process, the efficiency is improved by 35%-50%, the ton cost is reduced by 15%-20%, and the product is suitable for seed treatment and foliage spraying of various crops and has wide application prospects. The industrialization potential is remarkable, and the requirements of workers are met.

The invention relates to the technical field of food fermentation and food flavor improvement. The invention relates to a fermentation product, in particular to a flavor improving method of a daylily fermentation product and application thereof. The flavor improving method comprises the following steps: S1, carrying out fixation treatment on cleaned day lily, and pulping to obtain day lily slurry; s2, performing low-temperature plasma treatment on the day lily slurry obtained in the step S1; s3, inoculating pre-activated saccharomycetes and lactobacillus plantarum into the day lily slurry subjected to low-temperature plasma treatment, and fermenting in a fermentation environment to obtain a day lily fermentation product; wherein the generation of various volatile flavor substances in the day lily fermentation process is promoted through the synergistic regulation effect of the low-temperature plasma treatment and the microbial fermentation process, so that the aroma component types of the fermentation product are richer; meanwhile, excessive accumulation of low-molecular alcohol substances, such as ethanol, isobutanol and isoamyl alcohol, which are easy to generate pungent smell is inhibited, so that the overall flavor coordination and quality of the daylily fermented product are improved.

The invention provides a freeze-dried eye mask and a preparation method thereof, and the freeze-dried eye mask is prepared from the following components in parts by weight: 0.05 to 0.25 part of a glycoside nonionic surfactant, 0.03 to 0.25 part of phosphatidylcholine, 0.01 to 0.04 part of cholesterols, 0.03 to 0.2 part of a mould / saccharomycetes fermentation extraction product, 0.5 to 0.8 part of a humectant, 0.35 to 0.85 part of a skin conditioner, 0.01 to 0.015 part of a thickening agent, 0.25 to 0.4 part of an antiseptic bacteriostatic agent and 0.001 to 0.004 part of an acid-base regulator. Compared with the prior art, the freeze-dried eye mask disclosed by the invention has the advantages that components such as a skin conditioner can be entrapped in vesicles, permeation and absorption of functional components in eye skin can be improved, and the effects of removing wrinkles, whitening and the like are enhanced.

The invention relates to the technical field of biological fermentation, and discloses a production method and application of a chlorella yeast fermentation filtrate rich in exosome vesicles, and the production method comprises the following steps: chlorella culture, yeast culture, mixed fermentation and fermentation broth filtration. Wherein a culture medium containing a nitrogen source, a phosphorus source, a magnesium salt, a calcium salt, an iron salt, vitamins and the like is adopted for chlorella culture, inoculation is performed according to the volume ratio of 30%, and culture is performed at proper temperature and illumination; saccharomyces cerevisiae culture comprises activation, liquid culture medium preparation and liquid fermentation which are carried out under a sterilization condition, and shake culture is carried out at a proper temperature and a proper rotating speed after inoculation; the mixed fermentation comprises the following steps: mixing chlorella liquid with yeast liquid, supplementing a small amount of cane sugar, shaking, standing and culturing; and performing coarse filtration on the fermentation finishing solution by using filter paper and performing multi-stage filter membrane filtration to obtain a sterile filtrate rich in the chlorella saccharomycetes exosome. The production method disclosed by the invention is simple in culture condition, low in equipment requirement, low in production energy consumption and short in production period.

The invention discloses a yeast immunochromatography rapid detection test strip and a preparation method thereof, and relates to the technical field of immunodetection.The test strip comprises a functionalized sample pad, a combination pad, a reaction film and a water absorption pad which are in lap joint in sequence; the sample pad is pretreated by a treating fluid containing beta-1, 3-glucanase, casein and TritonX-100, so that in-situ wall breaking and interference resistance can be realized; the reaction film detection line is coated with mannose binding lectin and a saccharomycetes specific monoclonal antibody, the binding pad fixes different epitope detection antibodies labeled by colloidal gold, the preparation method comprises the steps of functionalizing the sample pad, coating the reaction film, preparing the binding pad and assembling the test strip, and the matched kit comprises the test strip and a phosphate buffer diluent with a specific pH value. The method can realize extraction-free one-step rapid detection of saccharomycetes, has both specificity and stability, and is suitable for multi-scene screening.

The invention belongs to the technical field of synthetic biology and fermentation engineering, and particularly relates to a coupling fermentation method for synthesizing single-cell protein from methanol and carbon dioxide. The coupling fermentation method comprises the following steps: mixing and stirring a fermentation medium and methanol according to a weight part ratio of (97-99): (1-3) to form a fermentation substrate mixed solution; mixing the pre-cultured saccharomycetes liquid, the pre-cultured photosynthetic bacteria liquid and the fermentation substrate mixed liquid according to a weight part ratio of (2-4): (3-6): (90-95), introducing a mixed gas source, and carrying out illumination fermentation to obtain fermentation liquid; and sequentially carrying out centrifugal separation and vacuum drying on the fermentation liquor to obtain single-cell protein. According to the method, the pre-cultured yeast liquid, the pre-cultured photosynthetic bacterium liquid and the fermentation substrate mixed liquid are mixed and then are subjected to aerated fermentation, so that the fermentation capacity of microorganisms at a relatively high fermentation temperature is improved, and the yield of single-cell protein is increased.

The invention discloses a water fermentation woody oil multi-component extraction method and related equipment, and solves the problems of low separation efficiency, poor environmental protection, complicated process, insufficient equipment adaptation and difficult tea saponin purification in tea seed extraction. Performing multi-stage pulping by a 400-mesh filter screen pulping machine, filtering residues to obtain fibers, centrifuging filtrate, washing with water, purifying and drying to obtain starch; adding 0.2% of saccharomycetes into the oil-water mixture, performing water fermentation at 45-50 DEG C for 12 hours for layering, adding ethanol into emulsified oil for demulsification, and recovering ethanol to obtain pure oil; the tea soap liquid is subjected to flocculation impurity removal, calcium salt precipitation-release and vacuum concentration drying, and tea saponin with the concentration being 95% or above is obtained; the special equipment comprises a base, a separation cup, a spacing-adjustable millstone, a lifting device and an automatic filter residue backflow device.

The invention discloses an intestinal flora regulating composition for targeting drug-resistant enterobacter and application thereof, and relates to the technical field of biological medicines and microecological preparations, in particular to an intestinal flora regulating composition for targeting drug-resistant enterobacter and application thereof, the active ingredients comprise bacillus capable of interfering a drug-resistant enterobacter quorum sensing system, bacillus capable of interfering a drug-resistant enterobacter quorum sensing system, bacillus capable of interfering a drug-resistant enterobacter quorum sensing system, and bacillus capable of interfering the drug-resistant enterobacter quorum sensing system. The bacillus subtilis can be bacillus coagulans or bacillus subtilis; saccharomycetes capable of adsorbing the drug-resistant enterobacter and consuming necessary trace elements thereof, such as saccharomyces cerevisiae with optimized cell walls; the oligosaccharide mixture can be utilized by beneficial bacteria and can generate inhibitory products after being subjected to specific enzymolysis by drug-resistant enterobacter, and galactooligosaccharide, xylooligosaccharide and seaweed-derived oligosaccharide are mixed according to a specific proportion. The composition can be used for reducing the loading capacity of drug-resistant enterobacter in intestinal tracts of mammals, restoring the balance of intestinal flora and reducing the systemic infection risk. The preparation method comprises the step of mixing the active ingredients with a pharmaceutically or food acceptable carrier to prepare an oral dosage form.

The invention discloses a xylooligosaccharide production method based on food-grade organic acid pretreatment and yeast purification, and belongs to the technical field of biotechnology and food processing. The method comprises the following steps: mixing a wood fiber raw material with a food-grade organic acid aqueous solution for pretreatment; carrying out enzymolysis reaction on the obtained pretreated mixture and xylanase, and carrying out solid-liquid separation to obtain solid residues and enzymatic hydrolysate; and finally, inactivating the obtained enzymatic hydrolysate, mixing the inactivated enzymatic hydrolysate with saccharomycetes for fermentation, and purifying the obtained feed liquid to obtain a xylooligosaccharide product. According to the method, food-grade organic acid is used, so that the method is safe and biodegradable and can be operated at a high solid-to-liquid ratio, and near-zero discharge of wastewater is realized; the yeast is used for fermentation, so that by-products such as residual organic acid and monosaccharide are effectively removed, and the purity of the xylooligosaccharide is remarkably improved.

The invention discloses preparation and application of a Chuzhou chrysanthemum exosome. The preparation method of the Chuzhou chrysanthemum exosome comprises the following steps: adding water or a buffer solution into Chuzhou chrysanthemum, and homogenizing to obtain Chuzhou chrysanthemum homogenate; inoculating mixed bacterium liquid containing lactobacillus, saccharomycetes and bacillus into the Chuzhou chrysanthemum homogenate, and fermenting to obtain Chuzhou chrysanthemum fermentation liquid; adding plasma activated water into the Chuzhou chrysanthemum fermentation liquor, and uniformly mixing and reacting to obtain a raw material solution; centrifuging the raw material liquid and taking supernate; and carrying out two-stage filtration on the supernatant, and collecting trapped fluid to obtain the Chuzhou chrysanthemum exosome. According to the preparation method, the extraction rate of the Chuzhou chrysanthemum exosome can be greatly increased by combining the specifically combined mixed bacteria fermentation with the plasma activation treatment, the high biological activity of the Chuzhou chrysanthemum exosome can be effectively maintained, and the Chuzhou chrysanthemum exosome can be applied to preparation of daily chemical products with free radical scavenging and anti-aging activity.

The invention discloses an engineering bacterium for producing sandalwood essential oil at high yield as well as a construction method and application of the engineering bacterium. The method comprises the following steps: carrying out the following modification on the saccharomycetes: increasing the copy number of SynSmFPS-SaSSy and SaA167-46tATR1 genes, and introducing the genes of CaTHL-ePTS1, tHMG1-ePTS1, EfmvaS-ePTS1, EfmvaE-ePTS1, MmERG12-ePTS1, ERG19-ePTS1, ERG8-ePTS1, IDI1-ePTS1, PEX11, 24tSaCYP736A167-46tATR1-PEX15, SynSmFPS-SaSSy-ePTS1, PDR3m, VHb and INO2, and carrying out the following modification on the saccharomycetes, so as to obtain the saccharomycetes. The engineering bacterium constructed by the invention can realize high yield of santalene and santalol, and has great economic value and social value in production of sandalwood essential oil.

The invention discloses a preparation process of a live microorganism fermented high-protein pet food, and relates to the technical field of animal feeds, prepared raw materials are added according to nutritional requirements, the raw materials are one or more of chicken meal, deep sea fish meal, low-temperature detoxified soybean meal and puffed soybean meal, the raw materials are subjected to live microorganism fermentation treatment, and the high-protein pet food is obtained. The live microorganisms are a combination of at least two of mould, bacillus, saccharomycetes and lactic acid bacteria, and sequential control is implemented based on synergistic fermentation of multiple live microorganisms. According to the invention, through sequential control of aerobic, facultative and anaerobic, growth characteristics of mould, bacillus, saccharomycetes and lactic acid bacteria are accurately adapted, and strain nutrition competition and metabolism antagonism are eliminated. The raw materials are enzymatically hydrolyzed by the mould, the bacillus degrades protein, the yeast optimizes flavor, the lactic acid bacteria inhibits bacteria and protects intestines, and the whole link synergistically exerts power, so that the defect that the function of a single strain is poor is avoided, and more efficient nutrition conversion and function enhancement effects are realized.

The invention discloses a process for brewing plateau highland barley vinegar by utilizing highland barley vinasse, and relates to the technical field of food fermentation. The characteristics of residual saccharomycetes, lactic acid bacteria and polysaccharide substances which are not completely degraded in the highland barley vinasse are adapted, infectious microbe pollution is effectively inhibited, fermentation stagnation is avoided, and the fermentation period is shortened; meanwhile, special flavor substances of the highland barley vinasse are fully excavated, the defects of single flavor, insufficient acidity and the like caused by direct application of the traditional process are overcome, and the flavor layering sense and the acidity stability of the product are improved; and finally, recycling and high-value utilization of the highland barley vinasse by-product is realized.

According to the method, firstly, high-proportion bamboo powder is used as a carbon source, and by means of comprehensive regulation and control of bamboo flavones, cellobiose synthesized through fermentation and the like in the bamboo powder, cellulase producing genes and the like of bamboo-decomposing bacteria are induced and activated, so that the bamboo-decomposing bacteria are obtained; meanwhile, the cultivation environment, self-space hybridization, fusion, seed liquid, an enzymolysis agent, a permeation stabilizer, strain enrichment and the like of the bamboo degradation strains are regulated and controlled, and combined bacteria such as bacillus subtilis, aspergillus oryzae, lactic acid bacteria, saccharomycetes, rhizopus and the like are prepared; then the bamboo decomposition combined bacteria synergistically and interactively efficiently degrade nitrogen source bamboo fibers in directional fermentation under substrate optimization and multi-dimensional comprehensive regulation and control conditions, the nitrogen source bamboo fibers are converted and synthesized into high-quality mycoprotein, digestible bamboo protein, soluble sugar and other nutrient substances, the repression effect in the bamboo decomposition fermentation process is eliminated, and the bamboo decomposition effect is improved. The problems of low fermentation strain activity, low adaptability, low efficiency, poor synergy, biological safety, stability and the like are solved.

The invention discloses a microbial agent for efficiently treating organic solid waste as well as a preparation method and application of the microbial agent, and relates to the technical field of fermentation engineering. The microbial agent comprises the following components in parts by weight: 50-70 parts of bacillus subtilis, 30-50 parts of bacillus, 10-30 parts of lactic acid bacteria, 10-30 parts of trichoderma, 10-30 parts of saccharomycetes, 5-10 parts of auxiliary materials, 5-10 parts of a protective agent and 1-5 parts of an additive. The invention further provides a preparation method of the microbial inoculum and application of the microbial inoculum in efficient treatment of organic solid waste. According to the microbial agent for efficiently treating the organic solid waste and the preparation method and application of the microbial agent, the organic solid waste can be effectively degraded, the degradation rate is high, harmful substances are not generated, energy is saved, environment friendliness is achieved, meanwhile, the survival rate of strains is high, storage and use are convenient, and the problems that an existing microbial agent is low in organic solid waste degradation rate, low in cost and the like can be solved. And the survival rate is poor.

The invention relates to the technical field of microbial fermentation, in particular to an extraction method of phycocyanin crude protein based on microbial fermentation and application of an obtained product. The method comprises the following steps: S1, dissolving spirulina powder in a phosphate buffer solution, and adding glucose to obtain an algae powder suspension; s2, inoculating saccharomycetes into the algae powder suspension, and culturing for 12-24 hours to obtain a first-stage fermentation product; s3, inoculating the first-stage fermentation product with plant lactobacillus, and continuing fermentation culture for 24-48 hours to reduce the pH value of the system; s4, after fermentation is finished, supernate is taken and concentrated through an ultrafiltration membrane with the molecular weight cut-off of 50 kDa, dialysis or nanofiltration desalination is carried out, and phycocyanin crude protein is obtained. The method for extracting the phycocyanin crude protein by utilizing the microorganisms to cooperatively ferment the spirulina powder is low in energy consumption, free of organic solvent and capable of being amplified, has remarkable technical effect improvement and process comprehensive advantages, and provides technical support for deep application in the fields of food and health.

The invention belongs to the field of microbial technology and medicine, and particularly relates to an adhesive engineering yeast probiotic preparation as well as a preparation method and application thereof. The engineering saccharomycetes for efficiently expressing cantharides yellow is constructed by applying a synthetic biological technology. On the basis, the adhesion engineering yeast probiotics MBPSc22 are prepared through noradrenaline auto-polymerization, and the survival rate and retention capacity of the engineering yeast in gastrointestinal tracts are improved while the macrophage targeting capacity of the engineering yeast is not damaged. The adhesive engineering probiotic preparation can express canthaxanthin, directionally remove active oxygen at an inflammation part, reduce the inflammation level, increase the abundance and diversity of intestinal microorganisms, optimize the structure of the intestinal microorganisms and recover the steady state of the intestinal microorganisms, so that the aim of treating colitis is fulfilled.

The invention relates to the technical field of biological fermentation and natural bacteriostasis of traditional Chinese medicinal materials, and discloses a preparation process of wormwood fermentation liquor. The process comprises the following steps: by taking wormwood as a raw material, cleaning, crushing and pretreating the wormwood, introducing a compound functional flora composed of lactic acid bacteria and saccharomycetes, implementing multi-stage synergistic fermentation under temperature-controlled and oxygen-controlled conditions, and regulating fermentation temperature, dissolved oxygen and pH parameters to promote release and biotransformation of bacteriostatic related components in the wormwood so as to form a stable bacteriostatic metabolism system. And after fermentation is finished, carrying out solid-liquid separation and stabilizing treatment to obtain the wormwood fermentation liquor with continuous bacteriostatic activity. The fermentation liquor has an inhibition effect on various bacteria and fungi, is controllable in process parameters, good in repeatability and suitable for large-scale production, and can be used in the fields of agricultural bacteriostasis, mildew prevention, corrosion prevention and the like.

The antioxidant anti-aging whitening essence is prepared from the following components in parts by mass: 0.1 to 0.5 part of leech extract, 2 to 6 parts of saccharomycetes fermentation product filtrate and 40 to 60 parts of rose extract, the saccharomycetes fermentation product filtrate is filtrate of saccharomycetes fermentation fruits; the fruits are one or more than two of apples, sour oranges and grapefruits; the rose variety of the rose flower extract is rosarugosa; the invention further discloses the preparation method of the antioxidant anti-aging whitening essence. The traditional Chinese medicine composition has good whitening, anti-oxidation, anti-aging, skin tightening and moisturizing effects, and can remove free radicals generated by skin cells, inhibit generation of melanin, fade existing color spots and acne marks, homogenize skin color, brighten skin color, improve skin elasticity and delay aging.

The invention discloses a fermentation process for producing craft wheat white beer and the craft wheat white beer, and belongs to the field of microbial fermentation, and the fermentation process comprises the following steps: mixing and fermenting cooled and oxygenated raw wheat juice and compound zymophyte in a fermentation tank with a metal ion slow release layer; the compound zymophyte comprises a first leavening agent and a capsule type second leavening agent, and both the first leavening agent and the second leavening agent are saccharomycetes; compared with existing primary fermentation, the fermentation period is properly prolonged through two times of fermentation, the secondary fermentation belongs to low-temperature slow fermentation, fermentation is more sufficient, more dense, stable and lasting cup hanging foam can be obtained, and under the condition that the fermentation period is properly prolonged, the fermentation effect is better. In order to make supplement of the zinc ions adapt to the whole long fermentation period, the metal ion slow release layer is laid at the bottom of the fermentation tank, slow release of the zinc ions is achieved, and excessive zinc ions can be avoided under the condition that the zinc ions are supplemented for a long time.

The invention discloses a ruminant overwintering concentrate supplement feed and a production method thereof. The ruminant overwintering concentrate supplement feed comprises the following components in parts by weight: 60-90 parts of a basic raw material, 5-15 parts of a cold-resistant stress additive, 1-5 parts of a probiotic compound, 1-5 parts of a flavor improving component and 4-8 parts of a mineral supplement, the probiotic compound comprises lactic acid bacteria, saccharomycetes and cellulose decomposition bacteria; the flavor improving component comprises molasses, vanilla powder, an acidifying agent and citric acid; the mineral supplement comprises calcium carbonate, calcium hydrophosphate, sodium chloride and compound trace elements, wherein the compound trace elements comprise iron, zinc, selenium and manganese. The basic raw materials comprise energy raw materials and protein raw materials, the ratio of the energy raw materials to the protein raw materials is 2: 1, and the energy raw materials are selected from at least two of corn, barley, wheat, wheat bran and rice bran; the protein raw materials are selected from at least two of soybean meal, cottonseed meal, rapeseed meal and peanut meal. The invention relates to the technical field of feed processing, and particularly provides a ruminant overwintering concentrate supplement feed and a production method thereof.

The invention belongs to the technical field of food, and particularly relates to rhizoma polygonati compound fermentation liquor as well as a preparation method and application thereof. The preparation method of the polygonatum sibiricum compound fermentation liquor specifically comprises the following steps: (1) taking dried polygonatum sibiricum tubers, crushing and sieving to obtain polygonatum sibiricum powder; adding water for soaking to obtain a rhizoma polygonati aqueous solution; (2) respectively culturing lactobacillus plantarum and saccharomycetes to obtain a lactobacillus plantarum solution and a saccharomycetes solution; (3) inoculating the lactobacillus plantarum liquid and the saccharomycetes liquid into a fermentation culture medium, and culturing to obtain a probiotic liquid; and (4) inoculating the probiotic liquid into the rhizoma polygonati aqueous solution for fermentation to obtain a fermentation mixture, centrifuging, and taking the supernatant. The content of total polysaccharide and saponin in the rhizoma polygonati compound fermentation liquor prepared by the preparation method is remarkably reduced, the content of monosaccharide is remarkably increased, the rhizoma polygonati compound fermentation liquor can be mixed with specific raw materials to prepare rhizoma polygonati rice vermicelli, functional components of rhizoma polygonati are effectively reserved, the taste and flavor of products are improved, and the rhizoma polygonati compound fermentation liquor has a wide application prospect.

The invention relates to a method for preparing a meat substitute by fermenting soybean fibers through edible fungi, and belongs to the technical field of food fermentation. The method comprises the following steps: activating edible fungi to obtain edible fungus inoculation liquid; according to the method, soybean fibers are inoculated with edible fungus inoculation liquid for fermentation culture, part of polysaccharides in the soybean fibers are degraded into monosaccharides and oligosaccharides by secreting lignocellulose degrading enzyme in the fermentation process of edible fungi, meanwhile, the edible fungi secrete fungus protein, and the monosaccharides and the oligosaccharides are degraded into monosaccharides and oligosaccharides. The undegraded polysaccharide plays a role of an adhesive to crosslink the residual soybean protein in the soybean fibers with the fungal protein to form a compact structure, namely the meat substitute is obtained. Cocoa edible saccharomycetes are further introduced for mixed fermentation of bean fibers to optimize the fermentation product, and the taste and nutritional value of the fermentation product are improved. The method preliminarily solves the inherent defects of soybean protein meat and fungus protein meat, is suitable for the field of development and application of meat substitutes, and has a wide application prospect.