48 results about "Lipid structure" patented technology

Methods and compositions are provided for the therapeutic use of Wnt proteins, where the Wnt protein is inserted in the non-aqueous phase of a lipid structure. In some embodiments the Wnt protein is presented in its active conformation on an outer liposome membrane or micelle. Pharmaceutical compositions of the present invention can be administered to an animal for therapeutic purposes. In some embodiments of the invention, the compositions are administered locally, e.g. by injection at the site of an injury. For certain conditions it is desirable to provide Wnt activity for short periods of time, and an effective dose will be administered over a defined, short period of time.

The invention discloses a colorless and transparent polyimide resin material. 1,2,3,4-cyclohexanetetracarboxylic dianhydride is chosen as dianhydride monomer or comonomer to carry out polycondensation reaction with primary diamine, so that the polyimide resin material is prepared. Because the 1,2,3,4-cyclohexanetetracarboxylic dianhydride comonomer has a distorted molecular structure, a large free volume exists between polymer molecular chains, and thereby the formation of charge transfer complex (CTC) in and between polyimide molecules is inhibited; and meanwhile, because of the introductionof a lipid structure, the electron-stimulated transition mode in the polyimide molecular chains is changed, the absorption of the aromatic polyimide in the visible light region is remarkably weakened, and thereby the transparency of the polymer is greatly increased. The ultraviolet light transmission cutoff wavelength of a produced polyimide film is 280nm to 380nm, the light transmissivity at 450nm is 86 to 94 percent, moreover, the glass-transition temperature is 250 DEG C to 400 DEG C, and the polyimide resin material has a good application prospect in the fields of flexible substrate materials for solar cells, flexible transparent conducting film substrate materials, liquid crystal display materials and the like.

A method of preparing lipid particles having an asymmetric lipid coating is described. The lipid composition of the outer lipid coating of the particles varies from the inner to outer surfaces. The asymmetric lipid particles are formed by preparing a lipid composition containing a charged lipid and a therapeutic agent, where the particles each have an outer lipid coating with an external lipid leaflet and an internal lipid structure. The particles are then incubated under conditions effective to remove the charged lipid from the external lipid leaflet, thus rendering the lipid coating asymmetric. The particles have the ability to their regain surface charge via translocation of the lipids.

New synthetic Lipid-A analogs based on monosaccharide (1) and disaccharide (2) derivatives were designed and prepared in the present invention. Both structures (1) and (2) incorporate novel lipid structures (3) and (4) that are not found in nature. Also, novel disaccharide Lipid-A structures (2) that incorporate novel contingents of uniform lipids and where R1, R4 and R5 are the same substitution group of structure (III) were synthesized. Liposome formulations containing totally synthetic components such as synthetic Lipid-A and synthetic lipopeptide derived from tumor-associated MUC1 mucin are described along with their therapeutic utility. Comparative test results of immunostimulating properties and toxicity of Lipid-A analogs (1) and (2) are included.

Composition and methods are described for low immunogenic protein formulations. An example of such a protein is antihemophilic factor (FVIII). The composition generally includes the protein, polypeptide or peptide, one or more agents that bind to epitope domains of the proteins to form a complex. Buffers containing salts may be used to stabilize this interaction. For example, Factor VIII and serine containing phospholipids in buffer salts containing Ca<2+> and Na<+> can be used to prepare protein-lipid structures. These complexes are useful for treatment of diseases such as Hemophilia. A method for the formation of novel non-liposomal structures is also disclosed.

The invention discloses a genetic engineering bacterium for producing a monophosphoryl lipid A as well as a construction method and application thereof, belonging to the field of a genetic engineering. The genetic engineering bacterium is E.coli W3110 delta lacI lacZ::FnlpxE, wherein the lacI takes place a deletion mutation to lose activity and an expression FnlpxE gene is knocked into the lacZ gene. According to the bacterial strain constructed in the invention, on the basis of keeping a lipid structure single, the production cost is also reduced; no exogenous resistance genes are introduced into the bacterial strain so that the industrial production of the bacterial strain is easier to realize in a large scale.

The invention discloses a whitening freckle-removing lipidosome and a preparation method and an application of the whitening freckle-removing lipidosome. A whitening freckle-removing lipidosome composition is prepared from 5-30% of whitening and freckle-removing ingredients, 1-40% of polyhydric alcohol, 1-25% of phospholipid, 1-25% of grease, 1-15% of an emulsifying agent and deionized water. Thewhitening and freckle-removing ingredients comprising various components are screened through a large number of experiments, experiment results show that the provided whitening and freckle-removing ingredients have obvious effects on reducing melanogenesis and the like; the various whitening and freckle-removing ingredients are coated by the lecithin, can much easily permeate into the deep layer of the skin as the structures of the lecithin and the lipid structure of the skin are quite similar, and have the advantage of better skin absorption; the whitening and freckle-removing ingredients coated by the lecithin are isolated from the air, so that the problem of oxidation stain is avoided, and the stability is improved. According to a whitening and freckle-removing product prepared by usingthe lipidosome, the whitening and freckle-removing ingredients are slowly released, and the effects of whitening and removing freckles for a long period of time are achieved.

A method of preparing lipid particles having an asymmetric lipid coating is described. The lipid composition of the outer lipid coating of the particles varies from the inner to outer surfaces. The asymmetric lipid particles are formed by preparing a lipid composition containing a charged lipid and a therapeutic agent, where the particles each have an outer lipid coating with an external lipid leaflet and an internal lipid structure. The particles are then incubated under conditions effective to remove the charged lipid from the external lipid leaflet, thus rendering the lipid coating asymmetric. The particles have the ability to their regain surface charge via translocation of the lipids.

The invention discloses a culture fluid that promotes the generation of a lipid structure of tissue engineered skin. A basic culture fluid is added with hydrocortisone, epidermal growth factors, isoproterenol, insulin, transferrin, triiodothyronine, adenine, vitamin C, tretinoin, cholera toxin, L-serine, L-novain, palmic acid, linoleic acid, arachidonic acid, bovine pituitary extract, bovine serum albumin, calcium chloride, penicillin, streptomycin and fungizone B, etc. Tissue engineered skin with mature and completely differentiated horny layer and good lipid structure can be obtained by cultivating keratinocyte in the culture fluid of the invention, the culture fluid has strong stability, long shelf life of about 12 months, simple and convenient preparation method, and convenient product synthesis and mass production.

The present disclosure relates to a drug delivery carrier containing a lipid structure or a polymer particle which is covalently bonded to a cell-penetrating AP-GRR peptide (SEQ ID NO 1) or is modified with the peptide chain containing the peptide. The present disclosure also relates to a composition containing the drug delivery carrier and a physiologically active ingredient encapsulated in the carrier. The drug delivery carrier of the present disclosure can effectively deliver macromolecules that are difficult to be delivered into cells, thereby improving the bioavailability of the macromolecules.

The invention relates to the technical field of cosmetics, in particular to a ceramide composition with a repairing function and a cosmetic. The invention discloses a ceramide composition. The ceramide composition comprises more than three of ceramide EOS, ceramide NS, ceramide NP and ceramide AP. It is accidentally found that compared with single use of one ceramide or two ceramides, the ceramidecomposition has a synergistic effect when the three or more ceramides are combined, all the components of the ceramide composition synergistically repair a multilayer lipid structure of ordered tissue between keratinocytes, a skin barrier is repaired and permeation of irritants is reduced, and therefore, the defensive property of the skin to external stimulation is improved, the water content ofthe cuticle of the skin is further remarkably improved, the water locking capacity of the skin is enhanced, and the problem that the skin becomes red is solved.

The invention relates to methods for effecting qualitative and quantitative changes in the functional moieties expressed at the surface of cells and multi-cellular structures, and functional lipid constructs for use in such methods. In particular, the invention relates to functional lipid constructs and their use in diagnostic and therapeutic applications, including serodiagnosis, where the functional moiety is a carbohydrate, peptide, chemically reactive group, conjugator or fluorophore.

The invention discloses an anti-aging cosmetic composition capable of preserving moisture and supplementing water, which relates to the technical field of cosmetics. The anti-aging cosmetic composition is prepared from the following components in parts by weight: 0.001 to 10 parts of compound vegetable oil; 0.1 to 20 parts of a fermentation product; 2 to 4 parts of nicotinamide; 2-4 parts of a citrus peel extract; 0.1 to 5 parts of soluble proteoglycan; 0.1-5 parts of a myrothamnus flabellifolia leaf / stem extract; 0.1 to 3 parts of PEG / PPG-14 / 7 dimethyl ether; and 0.01 to 0.3 part of sodium hyaluronate. Under the combined action of matching of all the components, the composition can go deep into the skin to moisturize and supplement water, and also can moisturize and supplement water on the surface of the skin; the skin barrier function can be enhanced, and the lipid structure is improved; in addition, the skin feeling and the use feeling are not too sticky, and long-acting moisturizing and deep moisturizing can be achieved.

The invention discloses cationic amino lipid as well as a synthesis method and application thereof, and belongs to the field of cell transfection. The structure of the cationic amino lipid is shown inthe specification. The synthesis reaction conditions are mild, the solvent selectivity is high, the reaction difficulty is greatly reduced, the yield is improved, the raw materials are simple and cheap, and the production cost is greatly reduced. The cationic amino lipid molecule provided by the invention has the characteristics of high efficiency and low toxicity, can achieve a good effect in serum, and greatly simplifies the operation steps of transfection.

The invention discloses an irinotecan-cholesterol succinic acid simple lipid ion pair, a liposome and a preparation method and application thereof. The cholesterol succinic acid simple lipid is used as a counter-ionic agent, and the active proton of carboxyl in the cholesterol succinic acid simple lipid structure transfers to the nitrogen of irinotecan amino group, a coordination bond is formed, an ion pair is formed with irinotecan, so that the lipid solubility of irinotecan is improved, and the simple preparation method is easily used for packaging the drug on a phospholipid layer of the liposome, and the drug loading and packaging efficiency are improved. According to the irinotecan-cholesterol succinic acid simple lipid ion pair, the liposome and the preparation method and applicationthereof, the hydrophobic ion pair technology is combined with the nano drug delivery system, the lipid solubility of the insoluble drugs is improved, nano-preparations are developed, the complexity ofthe preparation process is simplified, and the irinotecan-cholesterol succinic acid simple lipid ion pair, the liposome and the preparation method and application thereof is conducive to expanding the industrial production. In addition, irinotecan ion pair and the liposome releases faster in the meta-acid environment, certain pH sensitivity is achieved, good antitumor effect and safety are achieved, and good application is achieved.

The present invention relates to formulae for infants comprising large lipid globules and / or lipid globules with a coating of phospholipids for rendering the growth trajectory or body development during the first year of life more similar to that observed for human milk fed infants.

The present invention relates to formulae for infants comprising large lipid globules and / or lipid globules with a coating of phospholipids for rendering the growth trajectory or body development during the first year of life more similar to that observed for human milk fed infants.

Disclosed are saccharide and phosphocholine derivatives. The derivatives include azide and alkyne derivatives which form one end of a variable length carbon chain. The opposite end of the variable length carbon chain is covalently linked to the saccharide or phosphocholine. The saccharide may be, for instance, a maltoside. The alkyne and azide derivatives of the saccharides and phosphocholine may be reacted together to form amphiphilic molecules useful in cellular membrane studies and applications. By adjusting the length of the carbon chain, the biochemical and biophysical properties of the resultant 1,4-disubstituted 1,2,3-triazole compounds may be custom tailored for the intended application. Resultant molecules may form micelles, bicelle, lipid bilayers and other like structures useful in the isolation and purification of membrane bound or membrane associated proteins and biochemical components. The saccharides and phosphocholine molecules may be alternatively substituted as desired to provide additional flexibility in designing the desired end product.

Ultra-small Lipid Structures (USLS) with an average mean particle diameter of less than 100 nm are made using a single step process by diluting a hydro-organic solution containing lipids and passenger compounds. These particles are capable of sequestering the passenger molecules and self-assemble in a single process step into USLS. The USLS have applications in, for example, agricultural, cosmetics, pharmaceutical and food and beverage industries.

The invention discloses gold nanorod-lipid polymer vesicle with the transdermal delivery function and a preparation method and application of the gold nanorod-lipid polymer vescle. The nano vesica include the lipid polymer vesicle and a gold nanorod attached to the interior of the lipid polymer vesicle, the nano vesica good in stability have thermal responsiveness and good biocompatibility, the lipid structure on the uttermost side can be fused with skin keratinocytes to penetrate the skin to enter subcutaneous tumor site, good gene therapy and thermal therapy effect on melanoma on the surface of the skin is achieved, and the advantages of targeted controllable drug delivery is realized. Efficient gene therapeutic effect is achieved, and the gold nanorod-lipid polymer vesicle is quite possible to be good carrier for local delivery of gene drugs; the carrier system has wide application prospect in treating tumors of the melanoma of the skin through transdermal genes; new research thought and technical reference are provided for design of a further gene drug transdermal delivery system.

Disclosed area methods for fabricating three-dimensional artificial lipid biomembrane structure arrays with sufficient reaction area and high stability on a substrate in a simpler and easier manner. The methods use constituent lipids of real cell membranes and a plurality of microwells formed on a substrate. The methods can more effectively provide biomimetic three-dimensional lipid membrane structure arrays that possess structural and / or functional properties of cell membranes.

The invention discloses a WGX-50 liposome as well as a preparation method and application thereof, and the WGX-50 liposome is prepared from WGX-50, phospholipids, phytosterols, a lipid structure modifier, a stabilizing additive and deionized water. The preparation method comprises the following steps: dispersing the WGX-50, the phospholipids and the phytosterols in an organic solvent; carrying out rotary evaporation and vacuum drying to remove the organic solvent and form a dry film; adding a stabilizing additive and deionized water into the dry film, and carrying out hydration treatment and micronization treatment; and adding an aqueous solution of a lipid structure modifier into the solution obtained by micronization treatment, and mixing and stirring to obtain the WGX-50 liposome. WGX-50 is wrapped in a liposome form, so that the water solubility of WGX-50 can be effectively improved, and the application potential of WGX-50 can be improved; meanwhile, through combined use of the phytosterols, the lipid structure modifier and the stability additive, the stability of the prepared WGX-50 lipid is remarkably improved.

The present invention relates to a nanoparticle composite, which is endocytosed into cells and used to treat a disease, and to a manufacturing method therefor. More specifically, the present inventionrelates to a nanoparticle composite and a manufacturing method therefor, wherein the nanoparticle composite shows improved endocytosis efficiency by modifying the nanoparticle surface with a lipid-based material having high stability and excellent biocompatibility; the nanoparticle composite can attain direct passage through the cellular membrane as well as endocytosis, and can be effectively endocytosed into spheroid-shaped tumor cells, by having a tube-shaped lipid structure combined with a portion of the nanoparticle surface; and the nanoparticle composite can be easily mass-produced by using a top-down manner, in which, the lipid structure is not attached directly to the nanoparticle, but the binding of the nanoparticle and the lipid-based lipidome (bubble, liposome, etc.) is induced,and then the physical force is applied thereto to disrupt the lipidome, thereby forming the lipid structure on the nanoparticle surface.

Provided is an anesthetic composition for locally administrating an amide-type anesthetic into a subject in need thereof. The anesthetic composition has multilamellar vesicles with entrapped amide-type anesthetic prepared by hydrating a highly entrapped lipid structure comprising an amide-type anesthetic and a lipid mixture with an aqueous buffer solution at a pH higher than 5.5. Also provided is a method to prepare an anesthetic composition using a simpler and more feasible process for large-scale manufacture and for providing a high molar ratio of amide-type anesthetic to phospholipid content as compared to the prior art. This anesthetic composition has a prolonged duration of efficacy adapted to drug delivery.

Microarray platforms and methods of fabricating said microarrays without traditional high aspect ratio barriers used to define individual array elements are described herein. Self-assembled nanoshells were stabilized with a polymerized scaffold to enhance the stability in physiological conditions and serve as an optical transducer upon molecular recognition events. Soft photolithography combined with surface chemistry was developed for covalent immobilization of nanoshells onto the pre-patterned arrayed microspots for rapid multiplexed detection of membrane-binding analytes. This robust fabrication methodology is amenable for general lipid structures, and thus facilitates the integration of stable membrane architectures into diagnostic and prognostic platforms. In particular, the microarray platform may be used in diverse applications ranging from the detection of pathogens, such bacterial toxin in biological matrices, to cellular membrane studies.

A heat killed Porphyromonas gingivalis bacterium expressing a homogenous lipid A structure having a molecular negative mass ion of 1368, 1435 / 1449, or 1690 / 1768 is used as an immunomodulator. The immunomodulator may be used in combination with an antigen of interest to potentiate or restrain a host immune response toward the selected antigen.

A method of inserting a lipid-linked moiety into a lipid assembly, such as a planar lipid monolayer or bilayer, a spherical lipid vesicle, a micelle, or an emulsion envelope monolayer is described. In the method, the lipid assembly and the lipid-linked moiety are contacted in the presence of microwave irradiation to permit the lipid-linked moiety to become associated with the lipid assembly. In one embodiment, the lipid assembly is a liposome and the lipid-linked moiety is a lipid-polymer. Compositions comprised of a lipid layer and of a lipid-linked moiety, prepared in accord with the method, are also described.