50 results about "Essential aminoacid" patented technology

The invention relates to the technical field of microalgae heterotrophic culture and biomass proliferation, in particular to a chlorella pyrenoidosa heterotrophic high-density proliferation method. The specific technical scheme is as follows: the heterotrophic high-density proliferation method of chlorella pyrenoidosa comprises the following steps: pre-culture and heterotrophic propagation of chlorella pyrenoidosa: inoculating a chlorella pyrenoidosa solution into a BG11 culture medium, and culturing for 3-4 days in a constant-temperature shaking table with the temperature of 25 + / -1 DEG C, the rotating speed of 200rpm and the light-dark ratio of 0h: 24h to obtain a primary culture chlorella solution; inoculating the primary culture algae liquid into a pre-sterilized and optimized heterotrophic culture medium, and carrying out expanding culture under the same culture condition for 3-4 days to obtain a secondary expanding culture algae liquid; (2) a heterotrophic high-density culture stage: regularly supplementing a nutrient culture medium containing high-concentration glucose, nitrate and phosphate by adopting an index fed-batch feeding strategy; other conditions are kept consistent during culture; and (3) an in-situ protein accumulation induction stage: when the chlorella pyrenoidosa reaches an exponential growth end stage, adding paclobutrazol into the system, and keeping other conditions consistent while keeping exponential fed-batch culture. According to the method disclosed by the invention, the biomass of the chlorella pyrenoidosa is increased by about 128.6% compared with that of the traditional batch culture, the protein content can reach 49.14%, meanwhile, the configuration of essential amino acids is improved, and the requirements of high-end biomass resource application are met. The method has the advantages of simplicity and convenience in operation and high production efficiency, and is suitable for the fields of high-quality protein extraction and related biomass production.

The invention belongs to the technical field of microbial protein preparation, and particularly relates to a method for preparing microbial protein from crop straw and application of the microbial protein. According to the method, the crop straw is subjected to biomass conversion pretreatment, so that the accessibility and hydrolysis efficiency of cellulose are improved; on the basis, the pretreated biomass is subjected to enzymolysis through cellulase, cellulose and hemicellulose components in the biomass are converted into glucose and xylose, and the glucose and the xylose are utilized by saccharomycetes in the subsequent saccharomycetes fermentation process to synthesize the microbial protein. The microbial protein obtained through the method has the advantages of being rich in nutrition and high in essential amino acid content, and has wide application prospects in the fields of food and feed. Therefore, after the crop straw is subjected to chemical or physical-biological treatment, a new way is provided for high-value utilization of the crop straw on the basis of obtaining the nutrient-rich microbial protein.

The invention relates to the technical field of microbiology and food processing, in particular to pediococcus acidilactici and application of the pediococcus acidilactici in preparation of a brain-intestinal axis regulating efficacy metagen product.The high-activity pediococcus acidilactici DY-1 is separated out from natural fermentation pickles of super-polyselenium plant cardamine violifolia for the first time; the strain can be widely applied to fermentation of high-selenium plant-based food, can significantly promote glycolysis release of selenium-containing active substances, has good acid resistance, high protease activity and high lipase activity, can fully ferment plant-based fermentation raw materials, and has high yield. Glycolysis of macromolecular substances such as protein, saccharides and fat is facilitated, accumulation of active ingredients such as essential amino acid, unsaturated fatty acid, alkaloid and phenolic substances is promoted, and the edibility and functionality of the product are improved; and the organic selenium-rich metagen product prepared by sterilizing the fermentation mixture can synergistically improve human body cognition and emotion regulation regulated by microorganism-intestinal-brain axis.

The embodiment of the present invention discloses a method and device for comprehensive evaluation of the nutritional quality of shrimp. The present invention takes Litopenaeus vannamei in different aquaculture water environments (seawater, typical saline-alkali water, fresh water) as the research object, detects and analyzes the nutritional components such as water, ash, protein, fat, amino acids, fatty acids in its muscle, clarifies the differences in key nutritional index parameters of shrimp under different aquaculture water environments, uses multivariate statistical methods to screen key characteristic nutritional indicators of shrimp (protein, ash, essential amino acids, semi-essential amino acids, monounsaturated fatty acids and ω-6 unsaturated fatty acids), and establishes a comprehensive evaluation model for the nutritional quality of shrimp. It has been verified that the model has good accuracy and can effectively evaluate the nutritional quality of shrimp.

The invention provides a method for preparing a feed organic protein base material from chicken manure subjected to spectrum treatment through a synthetic biotechnology, and belongs to the technical field of resource utilization. The invention provides a preparation method of an organic protein base material, which comprises the following steps: carrying out spectrum treatment on chicken manure to obtain chicken manure subjected to spectrum treatment; adjusting the carbon-nitrogen ratio of the chicken manure subjected to spectrum treatment to (20-30): 1 to obtain a compost base material; mixing the compost base material with a microbial agent, and carrying out stacking fermentation to obtain an organic protein base material; the microbial agent comprises candida utilis and lactobacillus plantarum. According to the preparation method provided by the invention, the protein content in the chicken manure can be remarkably increased, and the ammonia emission in the chicken manure fermentation process is reduced. The content of protein and essential amino acid in the organic protein base material prepared by the preparation method is high, the organic protein base material contains probiotics, nutritional indexes and safety indexes meet feed standards, and the organic protein base material can be used for replacing soybean meal to be applied as livestock and poultry feed.

The invention relates to the technical field of cell biology, in particular to a culture medium for inducing neural stem cells and application thereof.The culture medium for inducing the neural stem cells comprises a neural ectoderm induction culture medium and a neural stem cell induction culture medium; the neural ectoderm induction culture medium is prepared from a basic culture medium, an N-2 additive, a B-27 additive, an inhibitor SB431542 and an inhibitor Dorsomorphin, the neural stem cell induction culture medium is prepared from a basic culture medium, an N-2 additive and a B-27 additive, the basic culture medium is composed of a DMEM / F12 culture medium, a Neurobase culture medium, GlutaMax and non-essential amino acid NEAA. The problems that neural stem cells obtained through an existing single-layer adherent induction method based on small molecules or growth factors are uneven in differentiation and low in purity are solved.

The invention relates to the technical field of microorganisms, in particular to mucor capable of producing fungal protein at high yield and application of the mucor. The Mucor sp. TOF UM disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.40903. The Mucor sp. TOF UM disclosed by the invention has the advantages that the Mucor sp. The strain can efficiently produce fungal protein, fermentation is carried out under different nitrogen source conditions, the mycelium protein content and the proportion of essential amino acid in the obtained mycelium are both larger than 50%, and the strain can be used as a carrier with rich protein content for producing alternative protein. Moreover, the strain can utilize abundant substrates, can fully utilize wide-source and low-cost substrate raw materials such as industrial and agricultural wastes as a carbon source or a nitrogen source to ferment and produce the fungal protein, and has wide application value in food production.

The invention provides a venison oligopeptide solution as well as a preparation method and application thereof, and belongs to the technical field of health food development. The preparation method of the venison oligopeptide solution comprises the following steps: taking venison as a raw material, performing degreasing treatment by using a degreasing enzyme solution, and removing an enzyme hydrolysis product; inoculating the degreased venison with a fermentation inoculant, carrying out solid state fermentation treatment and liquid state fermentation treatment, and filtering after fermentation is completed; carrying out enzymolysis treatment on the fermented venison by using a protease solution, taking supernate after enzymolysis, carrying out enzyme deactivation, and filtering to intercept a 5kDa component, so as to obtain a venison oligopeptide solution; the venison oligopeptide solution disclosed by the invention is high in polypeptide yield, the antioxidant activity and the content of essential amino acids are obviously improved, the venison oligopeptide solution oral liquid is further developed, and the oral liquid is rich in nutrition, free of fishy smell of venison, pleasant in flavor and unique in taste.

The invention provides an induced pluripotent stem cell culture medium and a culture method of induced pluripotent stem cells. The induced pluripotent stem cell culture medium comprises a basic culture medium, a composite additive A and a composite additive B, the composite additive A comprises 5-30 [mu] g / mL of recombinant human insulin growth factors, 1-5 ng / mL of recombinant human transforming growth factors, 50-200 ng / mL of recombinant human basic fibroblast growth factors, 7-20 [mu] g / mL of sodium selenite, 0.5-2 mg / mL of albumin, 50-70 [mu] g / mL of ascorbic acid and 5-20 [mu] g / mL of transferrin; the composite additive B is prepared from 50 to 300mg / mL of a Stock B solution, 0.5 to 5 percent (v / v) of a non-essential amino acid solution, 50 to 200ng / mL of DL-piperidinecarboxylic acid, 50 to 200mu g / mL of gamma-aminobutyric acid, 0.1 to 1 percent (v / v) of a lipid concentrated solution, 0.5 to 5 percent (v / v) of an L-alanyl-L-glutamine solution and 0.1 to 0.1 percent of mercaptoethanol. The culture medium for the induced pluripotent stem cells provided by the invention can ensure the self-renewal capability of the iPSCs in a long-term passage process; and the genetic stability of the iPSCs cells is optimized according to the genome integrity and pluripotent state.

The invention discloses a method for enhancing the nutritional value of microbial mycoprotein. Protein rich in essential amino acid is heterologously expressed in the recombinant kluyveromyces marxianus, and the contents of crude protein, essential amino acid required for feeding human bodies and animals and branched chain amino acid in yeast obtained by fermentation are increased to different extents, so that the nutritional value of mycoprotein is enhanced. The method can be used for producing high-quality microbial protein, and has wide application value in the fields of food and feed.

The invention provides a China-hemp peptide nutrition reduced protein bar and a preparation method thereof. The China-hemp peptide nutrition reduced protein bar comprises 10-20 parts of China-hemp peptide powder, 10-20 parts of resistant dextrin, 5-10 parts of chia seeds, 5-8 parts of psyllium husk powder, 6-10 parts of China-hemp seeds, 4-7 parts of pumpkin seed kernels, 1-2 parts of stevioside and 1-2 parts of China-hemp seed oil micro-capsule powder. The China-hemp peptide is applied to the fat-reducing protein bar, the proportion of branched-chain amino acid rich in the China-hemp peptide to essential amino acid is close to the metabolism requirement of a human body, the China-hemp peptide can directly participate in muscle protein synthesis, muscle catabolism is inhibited, the basal metabolic rate is effectively maintained in the fat-reducing period, and muscle loss is prevented. The resistant dextrin and the psyllium husk powder can prolong gastric emptying time and induce satiety hormone secretion, high-quality unsaturated fatty acid provided by the hemp seeds and the pumpkin seed kernels can regulate expression of lipid metabolism related genes and promote fat oxygenolysis, and conversion from carbohydrates to fat is reduced due to the rapid energy supply characteristic of the medium-chain triglyceride.

This invention provides a human embryonic stem cell expansion culture medium, comprising DMEM / F12 medium, ITS-A, HEPES, magnesium ascorbate phosphate, non-essential amino acids, bFGF, FGF2-G3, GlutaMAX, LY-333531, gentamicin sulfate, Y27632, and CHIR99021. Y-27632 inhibits pluripotent stem cell apoptosis, improves adhesion efficiency, maintains pluripotency and colony formation ability; CHIR99021 enhances the self-renewal of pluripotent stem cells and inhibits their differentiation; LY-333531 inhibits spontaneous differentiation of pluripotent stem cells; and gentamicin sulfate reduces the risk of contamination during culture. The culture medium provided by this invention is serum-free, free of allogeneic substances, has a simple composition, and offers excellent expansion speed, effectively maintaining long-term stable passage of human embryonic stem cells, providing stable technical support for scientific research.

The invention discloses a preparation method of composite meal replacement powder with multi-plant synergistic nutrition, and relates to the technical field of food nutrition and healthy food processing, and the preparation method comprises the processes of raw material pretreatment, drying treatment, crushing treatment and mixing. According to the preparation method of the multi-plant synergetic nutrition composite meal replacement powder, synergetic supply of high-quality protein, low-GI carbohydrate, dietary fiber, unsaturated fatty acid and multiple vitamins and mineral substances is achieved through scientific proportioning, the full-nutrition meal replacement requirement is met, and quinoa and pea protein are complementary to improve the essential amino acid spectrum; rS3 type resistant starch in the Chinese yam and cinnamyl aldehyde jointly participate in blood sugar regulation, and the phyllanthus emblica is rich in natural antioxidant substances, is beneficial to improving immunity, and is suitable for diabetes people, weight control people and lactose intolerant vegetarian diet people.

Microbial origin protein (MOP) is regarded as an alternative to feed and food grade protein, offering a sustainable solution (negligible land footprint and a water footprint 14 times lower than that of soybean) to partially support the increased supply needs. Our goal is to incubate methane-oxidizing bacteria (MOB) by using bubble column bioreactor (BCB) with internal gas recycling. The production process of microbial origin protein consists of 4 steps: (a) fermentation step using a bubble column bioreactor (BCB) with internal gas recycling (polypropylene with a thickness of 11 mm) and working volume of 20 L and column dimension of 11.8 cm as internal diameter (D) and 213.0 cm as height (H), and a H / D ratio of 18.05; (b) biomass harvesting and concentration using centrifugation during 15 min at 3500 rpm and 4°C; (c) High Pressure Homogenization (HPH) processing was used to homogenize the biomass in four passes at pressure levels of 150 MPa with a duration of 4 min between the different passes; and (d) Spray-drying of the biomass using a spray dryer equipped with a rotary atomizer and the atomization pressure was maintained at 0.6 MPa. The methanotrophic biomass obtained exhibited an outstanding nutritional value. More specifically, a high protein content (~62%) along with a balanced amino acids profile, covering all dispensable and non-dispensable AAs, was produced by this process. Moreover, the lipid content (~8%) was also comparable to other nutrient sources such as fishmeal (8%). All the results obtained, and all the parameters determined in the present study are sufficient to implement a bubble column bioreactor at industrial scale and to produce the microbial origin protein (MOP) for animal and human nutrition.

The invention is suitable for the technical field of food processing, and provides a modification method of millet protein and modified millet protein.The modification method of the millet protein comprises the steps that the millet protein and water are stirred to be uniform, then the mixture is placed in a boiling water bath to be subjected to incubation pretreatment, cooling is conducted, and protein suspension liquid is obtained; and adding lactobacillus plantarum into the protein suspension, carrying out shaking table incubation treatment, and carrying out vacuum freeze drying treatment to obtain the modified millet protein. According to the present invention, the millet protein is subjected to incubation treatment through the lactobacillus plantarum, such that the soluble protein content and the peptide content of the millet protein are significantly improved, the essential amino acid ratio in the millet protein is optimized, and the nutritional value of the millet protein is significantly enhanced. Besides, the modification method can improve the physical properties of the millet protein and optimize the gel network structure of the millet protein, is simple and convenient to operate, environment-friendly, reasonable in cost control and suitable for large-scale industrial production, and does not need to depend on complex equipment or expensive raw materials.

The present application relates to a novel Aspergillus oryzae CJ11100 strain and a mycelium produced therefrom. Using the novel Aspergillus oryzae CJI1100 strain of the present application, a mycelium including all the kinds of essential amino acids can be mass-produced. The mycelium of the present application has, as a complete protein, the advantage of being used in various foods, including meat substitutes.

Low-molecular-weight sericin, according to the present disclosure, can enhance absorption efficiency when applied to the skin and, especially, has excellent antioxidant and tyrosinase inhibitory effects, and thus can fundamentally inhibit the degradation of elastin and collagen, which maintain skin elasticity. In addition, sericin has all essential amino acids except for tryptophan and thus can even be effectively used in wound dressings or food, in addition to cosmetic products.

The invention discloses a compound rice soup preparation process and a preparation method thereof, and relates to the technical field of rice soup preparation. Raw material pretreatment: mixing and soaking rice, coarse cereals and compound components; composite enzymatic hydrolysis: adding alpha amylase, saccharifying enzyme and cellulase for enzymatic hydrolysis; performing low-temperature boiling and concentration: boiling the enzymatic hydrolysate, performing vacuum concentration, and adding probiotics and compound trace elements to obtain a rice soup mixed solution; homogenizing: filling the blended mixed solution into a homogenizer, and homogenizing twice under the condition of 15MPa; through compound raw material proportioning and an enzymolysis process, the content of protein in rice soup is increased by 30-40%, the content of essential amino acid is increased by 25-30%, the content of polysaccharide is increased by 15-20% compared with a traditional process, and various trace elements and functional components are introduced.

The red date and cricket health care wine is prepared from red dates, crickets and edible alcohol, the material ratio of the red dates to the crickets is 1: (1-3), and the material-liquid ratio of the total amount of the red dates and the crickets to the edible alcohol is 1: (20-30) g / mL. The synergistic effect of polyphenol and flavonoid antioxidant components in the red dates and essential amino acids and unsaturated fatty acids in the crickets is utilized; red date polysaccharides can enhance the water solubility of cricket proteins and promote human body absorption; flavonoid substances in the crickets and red date polyphenol form an anti-oxidation composite network, and the DPPH free radical scavenging capacity is jointly improved.

The present invention relates to a composition comprising a yeast single cell protein (SCP) product comprising at least about 14% (w / w) of essential amino acids per gram of dried yeast SCP product. Preferably, the yeast single-cell protein product is produced by using ethanol or molasses as a raw material. The yeast single cell protein product advantageously contains high ratios of unsaturated and saturated fatty acids.

The invention discloses a preparation method of vinasse pigeon meat, and belongs to the technical field of food. The corynebacterium glutamicum type I and the aspergillus niger are firstly used for inoculated fermentation, and then the sorghum vinasse is added for secondary fermentation, so that the maturing time of the vinasse pigeon meat can be shortened to 10 days, the risk caused by vinasse deterioration can be effectively reduced, and the unification of flavor can be maintained. Amino acid analysis shows that the method can improve the content of essential amino acids and flavor amino acids and improve the delicate flavor of the fermented vinasse pigeon meat. The research can provide a theoretical basis for optimization of a vinasse pigeon meat processing technology, standardized processing of flavor and a rapid fermentation process.

The invention discloses an astragalus membranaceus stem and leaf compound microorganism fermentation technology and application, the technology comprises the steps that microorganisms for fermentation are provided, and the microorganisms for fermentation comprise lactobacillus plantarum, bacillus subtilis and aspergillus niger; providing a fermentation substrate of stems and leaves of astragalus membranaceus; adding a solvent into the fermentation substrate of the stems and leaves of astragalus membranaceus to obtain a mixed solution, and fermenting the mixed solution; and drying the fermented sample to obtain a fermentation product. After fermentation, stems and leaves have strong acid fragrance, the pH value is obviously reduced, the material is more fluffy than that before fermentation, and the fermentation quality is higher. An enzyme system generated by the compound microorganisms degrades cellulose in stems and leaves, part of biomass components are degraded to generate protein, saccharides and the like, the content of essential amino acid and non-essential amino acid is increased, and animal growth is facilitated. Analysis on the antioxidant capacities of main active components, namely flavone, polysaccharide and saponin, in the stems and leaves of the astragalus membranaceus before and after fermentation finds that the ABTS antioxidant capacity and FRAP antioxidant capacity of the flavone and the polysaccharide can be enhanced through fermentation, and animal diseases can be prevented and treated.

This application relates to a method for inducing stem cells to differentiate into retinal pigment epithelial cells (RPE), comprising the following steps: culturing the pluripotent stem cells in a first culture medium before the pluripotent stem cells exhibit cell state decline after cell culture, wherein the first culture medium comprises DMEM medium, serum, β-mercaptoethanol, non-essential amino acids (NEAA), and L-glutamine. The method described in this application can significantly improve the efficiency of differentiation into RPE.

The invention discloses a processing technology of a functional beverage with mutton and apple tastes. The processing technology is characterized in that a meat beverage with a novel taste is produced, and the problem that protein thermal aggregation occurs after a meat product is heated is solved. The method comprises the main operation steps of pretreatment, homogenization, centrifugation, filtration, water bath heating and stirring, acidity adjustment, sweetness adjustment, apple juice addition and high-pressure steam sterilization. The preferable formula is as follows: 1000g of purified water, 500g of apples, 250g of mutton, 50g of white granulated sugar, 40g of starch, 30g of high fructose corn syrup, 0.8 g of sodium carboxymethyl cellulose, 0.3 g of sodium citrate and 0.2 g of citric acid. The meat beverage maintains uniform and stable texture, the added apple juice can neutralize the fishy smell of mutton, the product is endowed with sour and sweet fruit juice flavor, and the product is rich in a large amount of essential amino acids, also contains a large amount of mineral substances and trace elements, and has the effects of tonifying deficiency and strengthening spleen, strengthening physique and maintaining beauty and keeping young. The technological process has scientificity and reasonability, meets actual requirements and can be applied to industrial production.

The invention discloses a food additive for pet emotion relieving and a preparation method and application thereof, and belongs to the technical field of nutritional supplements.The food additive is prepared from, by weight, 10-15 parts of central inhibitory neurotransmitter, 27-35 parts of essential amino acid, 30 parts of plant source components and 20-33 parts of auxiliary materials. According to the pet food additive provided by the invention, natural components such as the central inhibitory neurotransmitter, the essential amino acid and the plant source component have a synergistic effect, so that the pet food additive has the double effects of quick sedation and long-acting compression resistance, in addition, the pet food additive provided by the invention is simple to prepare, the required product can be obtained only by simple mixing, and commercial popularization and application are facilitated.

The application provides a feed additive for promoting growth of shrimps and crabs, a feed and application thereof, and belongs to the technical field of aquaculture. The feed additive is prepared from cladocerans as core components in combination with a probiotic compound (Saccharomyces cerevisiae, Bacillus velezensis and Lactobacillus brevis). The cladocerans contain rich high-quality proteins, balanced essential amino acids, various vitamins, minerals and natural chitin, and the nutritional composition is highly matched with the growth demand of shrimps and crabs. The feed additive can improve the food attraction, digestion and absorption rate and nutritional value of the basic feed, effectively promote the growth speed of shrimps and crabs, improve the weight gain rate and feed conversion rate, and simultaneously improve the immunity and disease resistance of shrimps and crabs.

The invention discloses application of lactobacillus salivarius with bacteriostatic ability and capable of improving flavor of fermented milk in the fermented milk, and belongs to the technical field of microorganisms. The fermented milk prepared from the lactobacillus salivarius combined CCFM1332 can inhibit porphyromonas gingivalis, has a strong inhibition effect on the forming ability of salmonella typhimurium, staphylococcus aureus, pseudomonas aeruginosa and pseudomonas fluorescens biological membranes, and increases the content of essential amino acids and the content of flavor amino acids in the fermented milk; the proportion of bitter amino acids is reduced and the flavor of the fermented milk is improved. In addition, the CCFM1332 is high in acid production capacity, the pH can be rapidly reduced, the fermentation time is shortened, and the production efficiency is improved.