23 results about "Essential aminoacid" patented technology

The invention relates to the technical field of cell biology, in particular to a culture medium for inducing neural stem cells and application thereof.The culture medium for inducing the neural stem cells comprises a neural ectoderm induction culture medium and a neural stem cell induction culture medium; the neural ectoderm induction culture medium is prepared from a basic culture medium, an N-2 additive, a B-27 additive, an inhibitor SB431542 and an inhibitor Dorsomorphin, the neural stem cell induction culture medium is prepared from a basic culture medium, an N-2 additive and a B-27 additive, the basic culture medium is composed of a DMEM / F12 culture medium, a Neurobase culture medium, GlutaMax and non-essential amino acid NEAA. The problems that neural stem cells obtained through an existing single-layer adherent induction method based on small molecules or growth factors are uneven in differentiation and low in purity are solved.

The invention relates to the technical field of microorganisms, in particular to mucor capable of producing fungal protein at high yield and application of the mucor. The Mucor sp. TOF UM disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.40903. The Mucor sp. TOF UM disclosed by the invention has the advantages that the Mucor sp. The strain can efficiently produce fungal protein, fermentation is carried out under different nitrogen source conditions, the mycelium protein content and the proportion of essential amino acid in the obtained mycelium are both larger than 50%, and the strain can be used as a carrier with rich protein content for producing alternative protein. Moreover, the strain can utilize abundant substrates, can fully utilize wide-source and low-cost substrate raw materials such as industrial and agricultural wastes as a carbon source or a nitrogen source to ferment and produce the fungal protein, and has wide application value in food production.

The invention provides an induced pluripotent stem cell culture medium and a culture method of induced pluripotent stem cells. The induced pluripotent stem cell culture medium comprises a basic culture medium, a composite additive A and a composite additive B, the composite additive A comprises 5-30 [mu] g / mL of recombinant human insulin growth factors, 1-5 ng / mL of recombinant human transforming growth factors, 50-200 ng / mL of recombinant human basic fibroblast growth factors, 7-20 [mu] g / mL of sodium selenite, 0.5-2 mg / mL of albumin, 50-70 [mu] g / mL of ascorbic acid and 5-20 [mu] g / mL of transferrin; the composite additive B is prepared from 50 to 300mg / mL of a Stock B solution, 0.5 to 5 percent (v / v) of a non-essential amino acid solution, 50 to 200ng / mL of DL-piperidinecarboxylic acid, 50 to 200mu g / mL of gamma-aminobutyric acid, 0.1 to 1 percent (v / v) of a lipid concentrated solution, 0.5 to 5 percent (v / v) of an L-alanyl-L-glutamine solution and 0.1 to 0.1 percent of mercaptoethanol. The culture medium for the induced pluripotent stem cells provided by the invention can ensure the self-renewal capability of the iPSCs in a long-term passage process; and the genetic stability of the iPSCs cells is optimized according to the genome integrity and pluripotent state.

This invention provides a human embryonic stem cell expansion culture medium, comprising DMEM / F12 medium, ITS-A, HEPES, magnesium ascorbate phosphate, non-essential amino acids, bFGF, FGF2-G3, GlutaMAX, LY-333531, gentamicin sulfate, Y27632, and CHIR99021. Y-27632 inhibits pluripotent stem cell apoptosis, improves adhesion efficiency, maintains pluripotency and colony formation ability; CHIR99021 enhances the self-renewal of pluripotent stem cells and inhibits their differentiation; LY-333531 inhibits spontaneous differentiation of pluripotent stem cells; and gentamicin sulfate reduces the risk of contamination during culture. The culture medium provided by this invention is serum-free, free of allogeneic substances, has a simple composition, and offers excellent expansion speed, effectively maintaining long-term stable passage of human embryonic stem cells, providing stable technical support for scientific research.

Microbial origin protein (MOP) is regarded as an alternative to feed and food grade protein, offering a sustainable solution (negligible land footprint and a water footprint 14 times lower than that of soybean) to partially support the increased supply needs. Our goal is to incubate methane-oxidizing bacteria (MOB) by using bubble column bioreactor (BCB) with internal gas recycling. The production process of microbial origin protein consists of 4 steps: (a) fermentation step using a bubble column bioreactor (BCB) with internal gas recycling (polypropylene with a thickness of 11 mm) and working volume of 20 L and column dimension of 11.8 cm as internal diameter (D) and 213.0 cm as height (H), and a H / D ratio of 18.05; (b) biomass harvesting and concentration using centrifugation during 15 min at 3500 rpm and 4°C; (c) High Pressure Homogenization (HPH) processing was used to homogenize the biomass in four passes at pressure levels of 150 MPa with a duration of 4 min between the different passes; and (d) Spray-drying of the biomass using a spray dryer equipped with a rotary atomizer and the atomization pressure was maintained at 0.6 MPa. The methanotrophic biomass obtained exhibited an outstanding nutritional value. More specifically, a high protein content (~62%) along with a balanced amino acids profile, covering all dispensable and non-dispensable AAs, was produced by this process. Moreover, the lipid content (~8%) was also comparable to other nutrient sources such as fishmeal (8%). All the results obtained, and all the parameters determined in the present study are sufficient to implement a bubble column bioreactor at industrial scale and to produce the microbial origin protein (MOP) for animal and human nutrition.

The present application relates to a novel Aspergillus oryzae CJ11100 strain and a mycelium produced therefrom. Using the novel Aspergillus oryzae CJI1100 strain of the present application, a mycelium including all the kinds of essential amino acids can be mass-produced. The mycelium of the present application has, as a complete protein, the advantage of being used in various foods, including meat substitutes.

The invention discloses a compound rice soup preparation process and a preparation method thereof, and relates to the technical field of rice soup preparation. Raw material pretreatment: mixing and soaking rice, coarse cereals and compound components; composite enzymatic hydrolysis: adding alpha amylase, saccharifying enzyme and cellulase for enzymatic hydrolysis; performing low-temperature boiling and concentration: boiling the enzymatic hydrolysate, performing vacuum concentration, and adding probiotics and compound trace elements to obtain a rice soup mixed solution; homogenizing: filling the blended mixed solution into a homogenizer, and homogenizing twice under the condition of 15MPa; through compound raw material proportioning and an enzymolysis process, the content of protein in rice soup is increased by 30-40%, the content of essential amino acid is increased by 25-30%, the content of polysaccharide is increased by 15-20% compared with a traditional process, and various trace elements and functional components are introduced.

The present invention relates to a composition comprising a yeast single cell protein (SCP) product comprising at least about 14% (w / w) of essential amino acids per gram of dried yeast SCP product. Preferably, the yeast single-cell protein product is produced by using ethanol or molasses as a raw material. The yeast single cell protein product advantageously contains high ratios of unsaturated and saturated fatty acids.

The invention discloses an astragalus membranaceus stem and leaf compound microorganism fermentation technology and application, the technology comprises the steps that microorganisms for fermentation are provided, and the microorganisms for fermentation comprise lactobacillus plantarum, bacillus subtilis and aspergillus niger; providing a fermentation substrate of stems and leaves of astragalus membranaceus; adding a solvent into the fermentation substrate of the stems and leaves of astragalus membranaceus to obtain a mixed solution, and fermenting the mixed solution; and drying the fermented sample to obtain a fermentation product. After fermentation, stems and leaves have strong acid fragrance, the pH value is obviously reduced, the material is more fluffy than that before fermentation, and the fermentation quality is higher. An enzyme system generated by the compound microorganisms degrades cellulose in stems and leaves, part of biomass components are degraded to generate protein, saccharides and the like, the content of essential amino acid and non-essential amino acid is increased, and animal growth is facilitated. Analysis on the antioxidant capacities of main active components, namely flavone, polysaccharide and saponin, in the stems and leaves of the astragalus membranaceus before and after fermentation finds that the ABTS antioxidant capacity and FRAP antioxidant capacity of the flavone and the polysaccharide can be enhanced through fermentation, and animal diseases can be prevented and treated.

This application relates to a method for inducing stem cells to differentiate into retinal pigment epithelial cells (RPE), comprising the following steps: culturing the pluripotent stem cells in a first culture medium before the pluripotent stem cells exhibit cell state decline after cell culture, wherein the first culture medium comprises DMEM medium, serum, β-mercaptoethanol, non-essential amino acids (NEAA), and L-glutamine. The method described in this application can significantly improve the efficiency of differentiation into RPE.

The invention discloses a food additive for pet emotion relieving and a preparation method and application thereof, and belongs to the technical field of nutritional supplements.The food additive is prepared from, by weight, 10-15 parts of central inhibitory neurotransmitter, 27-35 parts of essential amino acid, 30 parts of plant source components and 20-33 parts of auxiliary materials. According to the pet food additive provided by the invention, natural components such as the central inhibitory neurotransmitter, the essential amino acid and the plant source component have a synergistic effect, so that the pet food additive has the double effects of quick sedation and long-acting compression resistance, in addition, the pet food additive provided by the invention is simple to prepare, the required product can be obtained only by simple mixing, and commercial popularization and application are facilitated.

The application provides a feed additive for promoting growth of shrimps and crabs, a feed and application thereof, and belongs to the technical field of aquaculture. The feed additive is prepared from cladocerans as core components in combination with a probiotic compound (Saccharomyces cerevisiae, Bacillus velezensis and Lactobacillus brevis). The cladocerans contain rich high-quality proteins, balanced essential amino acids, various vitamins, minerals and natural chitin, and the nutritional composition is highly matched with the growth demand of shrimps and crabs. The feed additive can improve the food attraction, digestion and absorption rate and nutritional value of the basic feed, effectively promote the growth speed of shrimps and crabs, improve the weight gain rate and feed conversion rate, and simultaneously improve the immunity and disease resistance of shrimps and crabs.

The invention discloses application of lactobacillus salivarius with bacteriostatic ability and capable of improving flavor of fermented milk in the fermented milk, and belongs to the technical field of microorganisms. The fermented milk prepared from the lactobacillus salivarius combined CCFM1332 can inhibit porphyromonas gingivalis, has a strong inhibition effect on the forming ability of salmonella typhimurium, staphylococcus aureus, pseudomonas aeruginosa and pseudomonas fluorescens biological membranes, and increases the content of essential amino acids and the content of flavor amino acids in the fermented milk; the proportion of bitter amino acids is reduced and the flavor of the fermented milk is improved. In addition, the CCFM1332 is high in acid production capacity, the pH can be rapidly reduced, the fermentation time is shortened, and the production efficiency is improved.

The present invention relates to a composition comprising a yeast single cell protein (SCP) product comprising at least about 14% (w / w) of essential amino acids per gram of dried yeast SCP product. Preferably, the yeast single-cell protein product is produced by using ethanol or molasses as a raw material. The yeast single cell protein product advantageously contains high ratios of unsaturated and saturated fatty acids.

The purpose of the present invention is to provide a protein-containing composition in which the free amount of essential amino acids is increased. By using a protein deamidase and a protease derived from the genus Chryseobacterium, it is possible to improve the free amount of essential amino acids, foamability, foam stability, and / or emulsibility of a protein-containing composition.

This application discloses a method for preparing water-saving surimi gel based on polyphenol-enzyme synergistic crosslinking and its application. The method includes: rinsing surimi raw materials no more than twice; sequentially adding EGCG and TGase to the rinsed surimi, utilizing their synergistic effect to induce protein crosslinking; and then filling and undergoing two-stage heating gelation to obtain the surimi gel. This application significantly improves the breaking strength, elasticity, water-holding capacity, and other textural properties of the surimi gel by introducing synergistic crosslinking of EGCG and TGase into the water-saving rinsing process, successfully overcoming the quality degradation problem caused by reduced rinsing. Simultaneously, the gel exhibits unique digestive-resistant and slow-release properties, delaying protein hydrolysis in the early stages of gastric digestion and ensuring the full release of essential amino acids, thus preserving its nutritional value. This application provides an effective solution for developing high-quality, high-nutrition, and environmentally friendly novel surimi products.

The invention relates to the field of cell culture, in particular to a totipotent stem cell culture medium and a preparation method of totipotent stem cells. The totipotent stem cell culture medium comprises a basic culture medium and a supplementary culture medium, the basic culture medium comprises a Knock-out DMEM culture medium and Matrigel matrigel, and the supplementary culture medium comprises fetal calf serum, a penicillin-streptomycin mixed solution, GlutaMAX, non-essential amino acid, beta-mercaptoethanol, a leukocyte inhibiting factor and a 2 '-flucytidine tripolymer. According to the totipotent stem cell culture medium disclosed by the embodiment of the invention, only the 2 '-fluorocytidine tripolymer is used as a chemical small molecule additive, and other conventional stem cell culture mediums are matched to realize efficient preparation and long-term maintenance of the mouse totipotent stem cells, and the obtained mouse totipotent stem cells are excellent and stable in totipotent state.

Methods for producing a meat substitute with plant-based fat contents are disclosed. The methods include the use of protein and / or polysaccharide polymer with fibrillar properties in combination with protein sources of essential amino acids such as plant-based protein. These polymers may be mixed together and then combined with an oil phase emulsion to produce a precursor emulsion. The precursor emulsion may then be processed into three-dimensional fiber structures that include muscle-like fibers with fat encapsulation of the proteins. A contemplated method for forming the fibers includes centrifugally spinning the precursor emulsion to form the fibers.

The invention provides an artificial compound feed for improving collagen deposition of schizothorax grahami, and belongs to the field of fish feeds. The artificial compound feed comprises the following raw materials: an animal protein source raw material, a plant protein source raw material, a fat source raw material, a sugar source, a premix and auxiliary materials, the premix is a mixture of eucommia ulmoides, broad beans, a yeast extract, proline and hydroxyproline. Through the synergistic effect of the premix and the basic raw materials, the collagen and protein deposition of the schizothorax grahami are improved. In the premix, the proline and the hydroxyproline provide key amino acid raw materials for collagen synthesis, the eucommia ulmoides and the broad beans activate a muscle collagen synthesis pathway, and the yeast extract promotes absorption of functional components and jointly promotes collagen accumulation. In the basic raw materials, animal and plant protein sources provide comprehensive essential amino acids, and sufficient raw materials are provided for protein synthesis; the sugar source provides energy, so that protein is prevented from being decomposed for energy supply; the yeast extract also improves the digestion and utilization rate of protein raw materials, and assists whole-fish protein deposition.

Disclosed are a culture medium and a culture method for in vitro expansion of primary cells of intestinal cancer. The culture medium comprises an initial culture medium, a Rho protease inhibitor, an antibiotic, gastrin, A8301, a non-essential amino acid, cholera toxin, an insulin-like growth factor-1, nicotinamide, insulin, fetal bovine serum, and an additive selected from at least one of a B27 additive and an N2 additive. By using the culture medium, effective and rapid expansion of the primary cells of intestinal cancer can be achieved. The expanded cells maintain the pathological characteristics of patients, and the culture success rate and the cell expansion rate of the primary cells of intestinal cancer are improved, which provide a research foundation for personalized treatment of the patients.

A protein preparation may be obtained from dried lipid extracted marine algae. The novel hydrolysate has high solubility and digestibility, low ash content, and high protein content (measured as g amino acid / g of product), with an essential amino acid profile that makes an excellent protein supplement for human consumption. The process utilizes a hydrolyzation reaction at high solids content to minimize water footprint and ash content, while increasing its solubility and digestibility.