36 results about "Essential aminoacid" patented technology

The invention relates to the technical field of microbiology and food processing, in particular to pediococcus acidilactici and application of the pediococcus acidilactici in preparation of a brain-intestinal axis regulating efficacy metagen product.The high-activity pediococcus acidilactici DY-1 is separated out from natural fermentation pickles of super-polyselenium plant cardamine violifolia for the first time; the strain can be widely applied to fermentation of high-selenium plant-based food, can significantly promote glycolysis release of selenium-containing active substances, has good acid resistance, high protease activity and high lipase activity, can fully ferment plant-based fermentation raw materials, and has high yield. Glycolysis of macromolecular substances such as protein, saccharides and fat is facilitated, accumulation of active ingredients such as essential amino acid, unsaturated fatty acid, alkaloid and phenolic substances is promoted, and the edibility and functionality of the product are improved; and the organic selenium-rich metagen product prepared by sterilizing the fermentation mixture can synergistically improve human body cognition and emotion regulation regulated by microorganism-intestinal-brain axis.

The invention provides a method for preparing a feed organic protein base material from chicken manure subjected to spectrum treatment through a synthetic biotechnology, and belongs to the technical field of resource utilization. The invention provides a preparation method of an organic protein base material, which comprises the following steps: carrying out spectrum treatment on chicken manure to obtain chicken manure subjected to spectrum treatment; adjusting the carbon-nitrogen ratio of the chicken manure subjected to spectrum treatment to (20-30): 1 to obtain a compost base material; mixing the compost base material with a microbial agent, and carrying out stacking fermentation to obtain an organic protein base material; the microbial agent comprises candida utilis and lactobacillus plantarum. According to the preparation method provided by the invention, the protein content in the chicken manure can be remarkably increased, and the ammonia emission in the chicken manure fermentation process is reduced. The content of protein and essential amino acid in the organic protein base material prepared by the preparation method is high, the organic protein base material contains probiotics, nutritional indexes and safety indexes meet feed standards, and the organic protein base material can be used for replacing soybean meal to be applied as livestock and poultry feed.

The invention relates to the technical field of cell biology, in particular to a culture medium for inducing neural stem cells and application thereof.The culture medium for inducing the neural stem cells comprises a neural ectoderm induction culture medium and a neural stem cell induction culture medium; the neural ectoderm induction culture medium is prepared from a basic culture medium, an N-2 additive, a B-27 additive, an inhibitor SB431542 and an inhibitor Dorsomorphin, the neural stem cell induction culture medium is prepared from a basic culture medium, an N-2 additive and a B-27 additive, the basic culture medium is composed of a DMEM / F12 culture medium, a Neurobase culture medium, GlutaMax and non-essential amino acid NEAA. The problems that neural stem cells obtained through an existing single-layer adherent induction method based on small molecules or growth factors are uneven in differentiation and low in purity are solved.

The invention relates to the technical field of microorganisms, in particular to mucor capable of producing fungal protein at high yield and application of the mucor. The Mucor sp. TOF UM disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.40903. The Mucor sp. TOF UM disclosed by the invention has the advantages that the Mucor sp. The strain can efficiently produce fungal protein, fermentation is carried out under different nitrogen source conditions, the mycelium protein content and the proportion of essential amino acid in the obtained mycelium are both larger than 50%, and the strain can be used as a carrier with rich protein content for producing alternative protein. Moreover, the strain can utilize abundant substrates, can fully utilize wide-source and low-cost substrate raw materials such as industrial and agricultural wastes as a carbon source or a nitrogen source to ferment and produce the fungal protein, and has wide application value in food production.

The invention provides an induced pluripotent stem cell culture medium and a culture method of induced pluripotent stem cells. The induced pluripotent stem cell culture medium comprises a basic culture medium, a composite additive A and a composite additive B, the composite additive A comprises 5-30 [mu] g / mL of recombinant human insulin growth factors, 1-5 ng / mL of recombinant human transforming growth factors, 50-200 ng / mL of recombinant human basic fibroblast growth factors, 7-20 [mu] g / mL of sodium selenite, 0.5-2 mg / mL of albumin, 50-70 [mu] g / mL of ascorbic acid and 5-20 [mu] g / mL of transferrin; the composite additive B is prepared from 50 to 300mg / mL of a Stock B solution, 0.5 to 5 percent (v / v) of a non-essential amino acid solution, 50 to 200ng / mL of DL-piperidinecarboxylic acid, 50 to 200mu g / mL of gamma-aminobutyric acid, 0.1 to 1 percent (v / v) of a lipid concentrated solution, 0.5 to 5 percent (v / v) of an L-alanyl-L-glutamine solution and 0.1 to 0.1 percent of mercaptoethanol. The culture medium for the induced pluripotent stem cells provided by the invention can ensure the self-renewal capability of the iPSCs in a long-term passage process; and the genetic stability of the iPSCs cells is optimized according to the genome integrity and pluripotent state.

The invention discloses a method for enhancing the nutritional value of microbial mycoprotein. Protein rich in essential amino acid is heterologously expressed in the recombinant kluyveromyces marxianus, and the contents of crude protein, essential amino acid required for feeding human bodies and animals and branched chain amino acid in yeast obtained by fermentation are increased to different extents, so that the nutritional value of mycoprotein is enhanced. The method can be used for producing high-quality microbial protein, and has wide application value in the fields of food and feed.

The invention provides a China-hemp peptide nutrition reduced protein bar and a preparation method thereof. The China-hemp peptide nutrition reduced protein bar comprises 10-20 parts of China-hemp peptide powder, 10-20 parts of resistant dextrin, 5-10 parts of chia seeds, 5-8 parts of psyllium husk powder, 6-10 parts of China-hemp seeds, 4-7 parts of pumpkin seed kernels, 1-2 parts of stevioside and 1-2 parts of China-hemp seed oil micro-capsule powder. The China-hemp peptide is applied to the fat-reducing protein bar, the proportion of branched-chain amino acid rich in the China-hemp peptide to essential amino acid is close to the metabolism requirement of a human body, the China-hemp peptide can directly participate in muscle protein synthesis, muscle catabolism is inhibited, the basal metabolic rate is effectively maintained in the fat-reducing period, and muscle loss is prevented. The resistant dextrin and the psyllium husk powder can prolong gastric emptying time and induce satiety hormone secretion, high-quality unsaturated fatty acid provided by the hemp seeds and the pumpkin seed kernels can regulate expression of lipid metabolism related genes and promote fat oxygenolysis, and conversion from carbohydrates to fat is reduced due to the rapid energy supply characteristic of the medium-chain triglyceride.

This invention provides a human embryonic stem cell expansion culture medium, comprising DMEM / F12 medium, ITS-A, HEPES, magnesium ascorbate phosphate, non-essential amino acids, bFGF, FGF2-G3, GlutaMAX, LY-333531, gentamicin sulfate, Y27632, and CHIR99021. Y-27632 inhibits pluripotent stem cell apoptosis, improves adhesion efficiency, maintains pluripotency and colony formation ability; CHIR99021 enhances the self-renewal of pluripotent stem cells and inhibits their differentiation; LY-333531 inhibits spontaneous differentiation of pluripotent stem cells; and gentamicin sulfate reduces the risk of contamination during culture. The culture medium provided by this invention is serum-free, free of allogeneic substances, has a simple composition, and offers excellent expansion speed, effectively maintaining long-term stable passage of human embryonic stem cells, providing stable technical support for scientific research.

Microbial origin protein (MOP) is regarded as an alternative to feed and food grade protein, offering a sustainable solution (negligible land footprint and a water footprint 14 times lower than that of soybean) to partially support the increased supply needs. Our goal is to incubate methane-oxidizing bacteria (MOB) by using bubble column bioreactor (BCB) with internal gas recycling. The production process of microbial origin protein consists of 4 steps: (a) fermentation step using a bubble column bioreactor (BCB) with internal gas recycling (polypropylene with a thickness of 11 mm) and working volume of 20 L and column dimension of 11.8 cm as internal diameter (D) and 213.0 cm as height (H), and a H / D ratio of 18.05; (b) biomass harvesting and concentration using centrifugation during 15 min at 3500 rpm and 4°C; (c) High Pressure Homogenization (HPH) processing was used to homogenize the biomass in four passes at pressure levels of 150 MPa with a duration of 4 min between the different passes; and (d) Spray-drying of the biomass using a spray dryer equipped with a rotary atomizer and the atomization pressure was maintained at 0.6 MPa. The methanotrophic biomass obtained exhibited an outstanding nutritional value. More specifically, a high protein content (~62%) along with a balanced amino acids profile, covering all dispensable and non-dispensable AAs, was produced by this process. Moreover, the lipid content (~8%) was also comparable to other nutrient sources such as fishmeal (8%). All the results obtained, and all the parameters determined in the present study are sufficient to implement a bubble column bioreactor at industrial scale and to produce the microbial origin protein (MOP) for animal and human nutrition.

The present application relates to a novel Aspergillus oryzae CJ11100 strain and a mycelium produced therefrom. Using the novel Aspergillus oryzae CJI1100 strain of the present application, a mycelium including all the kinds of essential amino acids can be mass-produced. The mycelium of the present application has, as a complete protein, the advantage of being used in various foods, including meat substitutes.

Low-molecular-weight sericin, according to the present disclosure, can enhance absorption efficiency when applied to the skin and, especially, has excellent antioxidant and tyrosinase inhibitory effects, and thus can fundamentally inhibit the degradation of elastin and collagen, which maintain skin elasticity. In addition, sericin has all essential amino acids except for tryptophan and thus can even be effectively used in wound dressings or food, in addition to cosmetic products.

The invention discloses a compound rice soup preparation process and a preparation method thereof, and relates to the technical field of rice soup preparation. Raw material pretreatment: mixing and soaking rice, coarse cereals and compound components; composite enzymatic hydrolysis: adding alpha amylase, saccharifying enzyme and cellulase for enzymatic hydrolysis; performing low-temperature boiling and concentration: boiling the enzymatic hydrolysate, performing vacuum concentration, and adding probiotics and compound trace elements to obtain a rice soup mixed solution; homogenizing: filling the blended mixed solution into a homogenizer, and homogenizing twice under the condition of 15MPa; through compound raw material proportioning and an enzymolysis process, the content of protein in rice soup is increased by 30-40%, the content of essential amino acid is increased by 25-30%, the content of polysaccharide is increased by 15-20% compared with a traditional process, and various trace elements and functional components are introduced.

The red date and cricket health care wine is prepared from red dates, crickets and edible alcohol, the material ratio of the red dates to the crickets is 1: (1-3), and the material-liquid ratio of the total amount of the red dates and the crickets to the edible alcohol is 1: (20-30) g / mL. The synergistic effect of polyphenol and flavonoid antioxidant components in the red dates and essential amino acids and unsaturated fatty acids in the crickets is utilized; red date polysaccharides can enhance the water solubility of cricket proteins and promote human body absorption; flavonoid substances in the crickets and red date polyphenol form an anti-oxidation composite network, and the DPPH free radical scavenging capacity is jointly improved.

The present invention relates to a composition comprising a yeast single cell protein (SCP) product comprising at least about 14% (w / w) of essential amino acids per gram of dried yeast SCP product. Preferably, the yeast single-cell protein product is produced by using ethanol or molasses as a raw material. The yeast single cell protein product advantageously contains high ratios of unsaturated and saturated fatty acids.

The invention discloses an astragalus membranaceus stem and leaf compound microorganism fermentation technology and application, the technology comprises the steps that microorganisms for fermentation are provided, and the microorganisms for fermentation comprise lactobacillus plantarum, bacillus subtilis and aspergillus niger; providing a fermentation substrate of stems and leaves of astragalus membranaceus; adding a solvent into the fermentation substrate of the stems and leaves of astragalus membranaceus to obtain a mixed solution, and fermenting the mixed solution; and drying the fermented sample to obtain a fermentation product. After fermentation, stems and leaves have strong acid fragrance, the pH value is obviously reduced, the material is more fluffy than that before fermentation, and the fermentation quality is higher. An enzyme system generated by the compound microorganisms degrades cellulose in stems and leaves, part of biomass components are degraded to generate protein, saccharides and the like, the content of essential amino acid and non-essential amino acid is increased, and animal growth is facilitated. Analysis on the antioxidant capacities of main active components, namely flavone, polysaccharide and saponin, in the stems and leaves of the astragalus membranaceus before and after fermentation finds that the ABTS antioxidant capacity and FRAP antioxidant capacity of the flavone and the polysaccharide can be enhanced through fermentation, and animal diseases can be prevented and treated.

This application relates to a method for inducing stem cells to differentiate into retinal pigment epithelial cells (RPE), comprising the following steps: culturing the pluripotent stem cells in a first culture medium before the pluripotent stem cells exhibit cell state decline after cell culture, wherein the first culture medium comprises DMEM medium, serum, β-mercaptoethanol, non-essential amino acids (NEAA), and L-glutamine. The method described in this application can significantly improve the efficiency of differentiation into RPE.

The invention discloses a food additive for pet emotion relieving and a preparation method and application thereof, and belongs to the technical field of nutritional supplements.The food additive is prepared from, by weight, 10-15 parts of central inhibitory neurotransmitter, 27-35 parts of essential amino acid, 30 parts of plant source components and 20-33 parts of auxiliary materials. According to the pet food additive provided by the invention, natural components such as the central inhibitory neurotransmitter, the essential amino acid and the plant source component have a synergistic effect, so that the pet food additive has the double effects of quick sedation and long-acting compression resistance, in addition, the pet food additive provided by the invention is simple to prepare, the required product can be obtained only by simple mixing, and commercial popularization and application are facilitated.

The application provides a feed additive for promoting growth of shrimps and crabs, a feed and application thereof, and belongs to the technical field of aquaculture. The feed additive is prepared from cladocerans as core components in combination with a probiotic compound (Saccharomyces cerevisiae, Bacillus velezensis and Lactobacillus brevis). The cladocerans contain rich high-quality proteins, balanced essential amino acids, various vitamins, minerals and natural chitin, and the nutritional composition is highly matched with the growth demand of shrimps and crabs. The feed additive can improve the food attraction, digestion and absorption rate and nutritional value of the basic feed, effectively promote the growth speed of shrimps and crabs, improve the weight gain rate and feed conversion rate, and simultaneously improve the immunity and disease resistance of shrimps and crabs.

The invention discloses application of lactobacillus salivarius with bacteriostatic ability and capable of improving flavor of fermented milk in the fermented milk, and belongs to the technical field of microorganisms. The fermented milk prepared from the lactobacillus salivarius combined CCFM1332 can inhibit porphyromonas gingivalis, has a strong inhibition effect on the forming ability of salmonella typhimurium, staphylococcus aureus, pseudomonas aeruginosa and pseudomonas fluorescens biological membranes, and increases the content of essential amino acids and the content of flavor amino acids in the fermented milk; the proportion of bitter amino acids is reduced and the flavor of the fermented milk is improved. In addition, the CCFM1332 is high in acid production capacity, the pH can be rapidly reduced, the fermentation time is shortened, and the production efficiency is improved.

The present invention relates to a composition comprising a yeast single cell protein (SCP) product comprising at least about 14% (w / w) of essential amino acids per gram of dried yeast SCP product. Preferably, the yeast single-cell protein product is produced by using ethanol or molasses as a raw material. The yeast single cell protein product advantageously contains high ratios of unsaturated and saturated fatty acids.

The invention belongs to the technical field of animal food processing, and particularly relates to a traditional Chinese medicine compound cat food for improving intestinal health and enhancing immunity as well as a preparation method and application thereof. The invention provides a traditional Chinese medicine compound cat food for improving intestinal health and enhancing immunity. The traditional Chinese medicine compound cat food comprises a basic ration, a compound premix and a traditional Chinese medicine formula, the basic ration comprises protein, carbohydrate, animal fat and dietary fiber; the compound premix comprises compound vitamins, compound trace mineral elements, essential amino acids, edible gels, antioxidants and mould adsorbents; the traditional Chinese medicine formula comprises artemisinic acid and / or rosmarinic acid. The traditional Chinese medicine compound cat food disclosed by the invention is comprehensive and balanced in nutrition, can provide required nutrient substances and various micronutrients for cats, improves the intestinal health, reduces the soft stool diarrhea rate, improves the organism immunity, and achieves the purposes of maintaining the body health of animals, preventing diseases, promoting disease rehabilitation and improving the life quality of pet cats.

The present application belongs to the technical field of microbial protein preparation, and particularly relates to a method for preparing microbial protein by using crop stalks and application thereof. The method improves the accessibility of cellulose and the hydrolysis efficiency by carrying out biomass conversion pretreatment on the crop stalks; on this basis, the pretreated biomass is subjected to enzymolysis by cellulase, so that the cellulose and hemicellulose components in the biomass are converted into glucose and xylose; and the glucose and xylose are utilized by yeast in the subsequent yeast fermentation process to synthesize microbial protein. The microbial protein obtained in this way has the advantages of rich nutrition and high content of essential amino acids, and has a wide application prospect in the fields of food and feed. It can be seen that the present application provides a new way for high-value utilization of crop stalks on the basis of obtaining rich-nutrition microbial protein through chemical or physical-biological treatment of the crop stalks.

The purpose of the present invention is to provide a protein-containing composition in which the free amount of essential amino acids is increased. By using a protein deamidase and a protease derived from the genus Chryseobacterium, it is possible to improve the free amount of essential amino acids, foamability, foam stability, and / or emulsibility of a protein-containing composition.

This application discloses a method for preparing water-saving surimi gel based on polyphenol-enzyme synergistic crosslinking and its application. The method includes: rinsing surimi raw materials no more than twice; sequentially adding EGCG and TGase to the rinsed surimi, utilizing their synergistic effect to induce protein crosslinking; and then filling and undergoing two-stage heating gelation to obtain the surimi gel. This application significantly improves the breaking strength, elasticity, water-holding capacity, and other textural properties of the surimi gel by introducing synergistic crosslinking of EGCG and TGase into the water-saving rinsing process, successfully overcoming the quality degradation problem caused by reduced rinsing. Simultaneously, the gel exhibits unique digestive-resistant and slow-release properties, delaying protein hydrolysis in the early stages of gastric digestion and ensuring the full release of essential amino acids, thus preserving its nutritional value. This application provides an effective solution for developing high-quality, high-nutrition, and environmentally friendly novel surimi products.

The present invention is directed to, inter alia, a method for culturing somatic cells with adherent growth properties in a suspension being support-free, wherein the somatic cells arc not genetically- or genomically-modified or -engineered, the method including contacting the somatic cells with an effective amount of a defined culture medium including: basal medium, serum replacement, and at least one non-essential amino acid (NEAA). Further provided are a defined culture medium, as well as a kit for culturing somatic cells with adherent growth propertied in a suspension being support-free.

The invention relates to the field of cell culture, in particular to a totipotent stem cell culture medium and a preparation method of totipotent stem cells. The totipotent stem cell culture medium comprises a basic culture medium and a supplementary culture medium, the basic culture medium comprises a Knock-out DMEM culture medium and Matrigel matrigel, and the supplementary culture medium comprises fetal calf serum, a penicillin-streptomycin mixed solution, GlutaMAX, non-essential amino acid, beta-mercaptoethanol, a leukocyte inhibiting factor and a 2 '-flucytidine tripolymer. According to the totipotent stem cell culture medium disclosed by the embodiment of the invention, only the 2 '-fluorocytidine tripolymer is used as a chemical small molecule additive, and other conventional stem cell culture mediums are matched to realize efficient preparation and long-term maintenance of the mouse totipotent stem cells, and the obtained mouse totipotent stem cells are excellent and stable in totipotent state.

The invention discloses an NK cell culture solution and an NK cell culture method. Comprising a basic culture solution, human serum, recombinant human interleukin-2, recombinant human interleukin-15, recombinant human interleukin-21, recombinant human stem cell factors, a recombinant human FMS-like tyrosine kinase 3 ligand, L-glutamine, beta-mercaptoethanol, non-essential amino acid and sodium pyruvate, wherein the basic culture solution is an IMDM culture solution; the invention relates to the technical field of cell culture. According to the NK cell culture solution and the NK cell culture method, a proper environment is provided for growth, proliferation and activity maintenance of NK cells through reasonable matching of various cell factors, nutritional ingredients and the like, and the culture efficiency of the NK cells can be remarkably improved; meanwhile, all the components in the culture solution have a synergistic effect, the activity and functional stability of the NK cells are effectively maintained, and the culture method is used for culturing the NK cells with the culture solution, operation is easy, and repeatability is high.