306 results about "Hypsizygus marmoreus" patented technology

The invention discloses a method for identifying industrially cultivated strains of hypsizygus marmoreus, namely identifying industrially cultivated strains of hypsizygus marmoreus by using an SSR marker in a molecular marker method. In the method, the known DNA sequence of the close edible fungus species of the hypsizygus marmoreus is adopted, the SSR primer sequence is designed and screened and the SSR marker map is successively built, thus realizing identification of the industrially cultivated strains of hypsizygus marmoreus. The method can contribute to rapidly, accurately and economically identifying the industrially cultivated strains of hypsizygus marmoreus.

The invention provides a cultivation method of hypsizygus marmoreus. The cultivation method comprises the steps of filling, sterilizing, cooling, inoculating, culturing, mycelium stimulation, inducement to primordium, bearing and harvesting, wherein in the step of filling, raw materials are filled in cultivation bottles in a cultivation basket; 25 cultivation bottles are filled in the cultivation basket; the cultivation bottles are distributed in a layout of 5x5; and the volume of each cultivation bottle is 500-700ml. The cultivation method has the following beneficial effects: by reducing the volume of the cultivation bottles and increasing the quantity of cultivation bottles filled in the same cultivation basket, under the condition of ensuring that the hypsizygus marmoreus yield of one cultivation basket is basically equal, the cultivation cycle is substantially shortened, and vast invested construction area and corresponding capitals and goods are saved, thus reducing the cultivation cost of hypsizygus marmoreus; and on the premise that the size of the basket body is basically equal, the space utilization rate in the culture stage of hypsizygus marmoreus can be increased by using the cultivation method provided by the invention instead of the existing cultivation method.

The invention relates to a factorization strain production method for hypsizygus marmoreus and a cultivation method for the hypsizygus marmoreus. The factorization strain production method for the hypsizygus marmoreus comprises inserting inclined plane stock culture into a flat plate to cultivate for 12-14 days at the temperature of 22 DEGC to 25 DEG C; then transferring flat plate strains into a shake flask filled with shake flask culture media to cultivate for 6-8 days at the temperature of 22 DEGC to 25 DEG C to obtain shake flask strains; then transferring the shake flask into a fermentation tank filled fermentation media, keeping temperature at 22 DEGC to 25 DEG C, maintaining ventilation pressure at 0.05-0.15MPa, and cultivating for 6-8 days to obtain fermentation tank liquid strains; inserting the fermentation tank liquid strains into a stock bottle filled with stock materials to cultivate for 35-40 days in dark to achieve stock, and picking and sterilizing to obtain factorization strains of the hypsizygus marmoreus. The factorization strain production method for the hypsizygus marmoreus has the advantages of being short in period and high in quality, can guarantee advantages of solid strains on mycelium stimulation mode of the hypsizygus marmoreus and hypsizygus marmoreus output quality and has good popularization value.

The invention discloses a method for producing a protein feed through hypsizygus marmoreus fungus chaff solid state fermentation and the protein feed being produced via the method for producing the protein feed through hypsizygus marmoreus fungus chaff solid state fermentation. According to the method, hypsizygus marmoreus is selected to cultivate the produced waste material-pollution-free hypsizygus marmoreus fungus chaff, the ground hypsizygus marmoreus is combined with bran, urea and the like, candida utilis, candida tropicalis and geotrichum candidum are inoculated according to a certain rate so as to ferment under an appropriate condition, and the feed which is rich in protein is obtained. After assay determination, the protein feed contains 20%-40% of crude protein which is increased by 1.5-4 times compared with the crude protein of unfermented hypsizygus marmoreus fungus chaff; the crude fiber content is between 15% and 18% which is reduced by 2-3.5 times of the crude fiber content of the unfermented hypsizygus marmoreus fungus chaff, so that the protein feed is a hypsizygus marmoreus fungus chaff protein feed which has rich nutrition, high protein and low fiber and is beneficial to the utilization and absorption of livestocks. By the method, the feeding cost of farmers can be lowered, waste utilization is realized, and an environmental protection function is obtained.

The invention belongs to the technical field of edible mushrooms and particularly relates to a hypsizygus marmoreus Finc-B-3 strain and application thereof. The hypsizygus marmoreus strain provided by the invention is named as Finc-B-3 and is obtained by taking a strain TNN-12 and a strain F-FG as parents to be hybridized through systematic selection, belongs to hypsizygus marmoreus and is preserved in Institute of Microbiology Chinese Academy of Sciences, wherein the address is No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.11204 and the preservation date is 20150813. Compared with the prior art, the hypsizygus marmoreus Finc-B-3 strain provided by the invention has short culture and cultivation periods, so that the production efficiency of factories is greatly improved; the unit yield is high, the yields of bottles are similar, and topographic characteristics are good, so that sub-packaging and sales of products are very easy; and the Finc-B-3 has good comprehensive performances and a wide market prospect.

The invention discloses a hypsizygus marmoreus liquid strain fermenting technique, comprising the following steps of: culturing a test tube seed; transferring the test tube seed to a shake flask; culturing the liquid strain in the shake flash; inoculating the strain of the shake flash in a fermentation tank; and then inoculating the strain of the fermentation tank in a strain package. The technique disclosed by the invention has the following advantages that the activity of the strain is high, the seed production period is short, the inoculating speed is high, the strain ages are the same, the pollution rate is low and the like; the liquid strain can be inoculated by using an inoculating gun, so that the semi-mechanization is realized, the efficiency is high, the manpower is saved, and the technique is suitable for being developed in the field of edible mushroom cultivation.

The invention discloses a selenium-enriched foliar fertilizer for paddy rice. The selenium-enriched foliar fertilizer for the paddy rice is prepared from the following raw materials in parts by weight: 6-8 parts of hypsizygus marmoreus, 10-12 parts of pleurotus geesteranus, 1.1 -1.3 parts of radix astragali, 1.2-1.4 parts of rhizoma cyperi, 0.6-0.8 parts of aster, 0.7-0.9 parts of california burclover herb, 1.3-1.5 parts of Speranskia cantonensis, 3-5 parts of garlic, 1-2 parts of beer yeast, 4-6 parts of wheat germs, 7-9 parts of clam meat, 14-16 parts of pork liver, 0.42-0.44 parts of compound sugar alcohol and 0.31-0.33 parts of compound enzyme. The raw materials of the selenium-enriched foliar fertilizer for the paddy rice contain multiple traditional Chinese medicinal ingredients with rich selenium elements, are scientific in compatibility, and supplement each other in function; not only can selenium element absorption and utilization of the paddy rice be facilitated, but also the pest rate of rice plant hoppers and the disease rate of rice blasts of the paddy rice can be effectively decreased, so that the yield of the paddy rice is improved; the clam meat and the pork liver with rich selenium elements are added into the raw materials; the adhesive force, on leaf surfaces of the paddy rice, of the foliar fertilizer can be effectively improved; an occurrence of a status that a foliar fertilizer falls off from the leaf surfaces after being applied in a spraying manner is effectively avoided, so that an action effect, on the paddy rice, of the foliar fertilizer is enhanced.

The invention relates to a culture medium, a cultivation medium and a cultivation method for Hypsizygus marmoreus liquid strain cultivation. The culture medium includes a PDA medium, a visual bottle medium, a shake flask medium and a fermenter medium, wherein the shake flask medium includes the following materials of glucose, yeast extract, monopotassium phosphate, magnesium sulfate, vitamin B, lignin powder and the water, the fermenter medium includes the following materials of soybean meal, corn flour, yeast extract, glucose, magnesium sulfate, monopotassium phosphate, defoamer, lignin powder and the water, and the culture medium includes the following materials of cottonseed hulls, wood chips, corn flour, soybean meal, bran, lime, gypsum powder and the water. The culture medium is advantaged in that the cultivation time of the Hypsizygus marmoreus can be shortened, the conversion rate of the culture medium is improved, cost is reduced, and the yield is improved.

Water retaining agent for edible fungi is prepared from raw materials including, by weight, from 40 to 80 parts of super-absorbent resin, from 50 to 60 parts of humate, from 10 to 20 parts of deionized water, from 10 to 20 parts of acrylic acid, from 2 to 4 parts of calcium hydroxide, from 30 to 40 parts of trace elements and from 20 to 30 parts of plant growth regulator. Moisture can be repeatedly released and absorbed, and certain heat-insulation and breathability functions are realized. Nutritious matters required by growth of the edible fungi can be provided, simultaneously, the edible fungi are urged to absorb moisture, and the price of products is low. The water retraining agent is wide in application range, and is applicable to material mixed and bagged cultivation of edible fungi, including oyster mushroom, shitake mushroom, ganoderma lucidum, coprinus comatus, pleurotus eryngii, pleurotus nebrodensis, agrocybe chaxingu, hypsizygus marmoreus, needle mushroom, agaric, hericium erinaceus, agaricus bisporus, button mushroom and the like.

The invention provides a hypsizygus marmoreus strain and a breeding method thereof. A hybrid strain is obtained by the polyspore hybridization of seafood mushroom and crab mushroom, and the hypsizygusmarmoreus variety Hypsizygus marmoreus No.1 is obtained by the polyspore selfing of the hybrid strain. According to the invention, the Hypsizygus marmoreus No.1 is obtained through hybridization andselfing breeding. The growth speed of the Hypsizygus marmoreus No.1 is faster than that of the parent strain, the bag filling time is 5 days earlier than that of the original strain of the seafood mushroom, the growth period is 10 days earlier than that of the parent seafood mushroom, the Hypsizygus marmoreus No.1 is bred and harvested 10 days earlier, the average mushroom stem length is 14.4 cm,the average mushroom cover diameter is 1.4 cm, the average single-bottle yield is 330.5g, the average biological efficiency is 110.1%, the Hypsizygus marmoreus No.1 is superior to the parent originalstrain of the seafood mushroom and the crab mushroom, and the economic benefit is improved.

The invention discloses a biofertilizer with edible fungus residue as the raw material and preparation thereof. The invention relates to a biofertilizer with edible fungus residue as the raw material, and the biofertilizer comprises the following components by weight: 20-40 parts of pretreated Hypsizygus marmoreus fungus residue, 1-3 parts of matrine, and 4-10 parts of fermented cow dung. The fertilizer provided by the invention can enhance the drought resistance of crops, is especially suitable for use in saline-alkali soil, can improve soil and improve soil physical properties, and is beneficial to improving soil fertility.

The invention belongs to the technical field of edible fungus culture, and relates to a Hypsizygus marmoreus culture medium. The culture medium comprises the following raw materials in parts by weight: 20-30 parts of wood dust, 10-16 parts of corn cob, 6-10 parts of bagasse, 23-28 parts of palm thread, 3-7 parts of bean pulp, 10-13 parts of bran, 0.5-1 part of lime and 0.5-1 part of gypsum powder. The Hypsizygus marmoreus cultured by the culture medium has the advantages of high nutrient content, short growth cycle and simple and rough culture process, and saves the labor force.

The invention discloses a brown hypsizygus marmoreus strain with high yield of glucan as well as a cultivation method and an application of the brown hypsizygus marmoreus strain. The glucan content of fruiting bodies of the brown hypsizygus marmoreus strain Finc-B-7 with high yield of glucan is 5%-10% on dry basis, the culture and cultivation period of the fruiting bodies is short, the per unit area yield is high, the production efficiency is improved greatly, and the production cost is reduced; the appearance uniformity of single strains as well as single fruiting bodies in single strain is high, and mechanized and automated operation is facilitated; pileus patterns are quite clear, the appearance is attractive, the commodity traits are good, the refreshing time is long, storage is facilitated, the overall characteristics of the strain are excellent, more strains with excellent characteristics can be cultured with the strain as an important parent, and the strain is quite suitable for large-area popularization for planting and has broad market prospect.

The invention relates to crab-flavored essence free of seafood allergen component. The crab-flavored essence comprises the following components in parts by weight: 92-99 parts of heat-reaction crab-flavored paste and 1-8 parts of crab-flavored essence base, wherein the heat-reaction crab-flavored paste comprises the following components in parts by weight: 60-80 parts of hypsizygus marmoreus enzymatic hydrolysate, 6-15 parts of table salt, 2-8 parts of amino acid, 1-8 parts of reducing sugar, 3-6 parts of corn starch, 1-5 parts of monosodium glutamate, 1-4 parts of white granulated sugar, 1-3 parts of disodium 5'-ribonucleotide and 1-6 parts of yeast extract. The essence takes the hypsizygus marmoreus as the main raw material, and deep processing and application are carried out on the hypsizygus marmoreus, and the method has profound significance on the development of the hypsizygus marmoreus industry, moreover, the essence does not contain any seafood allergen, and is thick in crab flavor and delicious in taste, high in simulation degree, rich in nutrition, natural and safe, and capable of meeting the eating requirements of consumers, the blank that plant protein enzymatic hydrolysate is not researched and applied to prepare the crab-flavored essence at present is filled, and the deep processing of the edible mushrooms is promoted.

The invention relates to an amino acid sequence, a gene sequence and an expression vector of a heat shock protein HmHSP70 of hypsizygus marmoreus, and belongs to the field of molecular biology. A complete open reading frame of the heat shock protein is acquired by RACE-PCR technology, and the amino acid sequence coded by the open reading frame is speculated; prokaryotic expression of the gene in colon bacillus makes the colon bacillus obtain obvious heat resistance so as to prove that the obtained gene sequence is genes of the heat shock protein; and the acquired gene provides new element for researching the heat shock protein, and the gene can be applied to transgene engineering to make a host transplanted into the gene obtain the heat resistance.

The present invention discloses an instant hypsizygus marmoreus soy protein powder which uses the fresh hypsizygus marmoreus or hypsizygus marmoreus leftover materials as main raw materials. The raw materials are subjected to washing, crushing, freezing, homogenizing for cell wall breaking, centrifugal separation, ultrafiltration concentration, and vacuum freeze-drying or spray drying, and the obtained materials are mixed with soy protein powder in proportion, and packaged to obtained the instant hypsizygus marmoreus soy protein powder. The instant hypsizygus marmoreus soy protein powder opens up a new way for the deep processing application of the hypsizygus marmoreus and expands application ranges of the market. The instant hypsizygus marmoreus soy protein powder is rich in hypsizygus marmoreus polysaccharides, can be consumed daily, and can enhance immunity, regulate blood pressure and lipid, clear liver and improve eyesight, and prevent and prevent against cancer.

The present invention discloses a crab flavor seasoning sauce prepared through an enzymolysis method, and a preparation method thereof, wherein the crab flavor seasoning sauce is prepared from the following raw materials by weight: 40 parts of a crab meat hydrolysate, 60 parts of a hypsizygus marmoreus hydrolysate, 10-20 parts of amino acids, 10-20 parts of reducing sugar, 5-15 parts of edible salt, 5-10 parts of disodium ribonucleotide, 5-10 parts of disodium succinate, 5-10 parts of caramel color, and 20-30 parts of water. The preparation method comprises: (1) preparing a crab meat hydrolysate; (2) preparing a hypsizygus marmoreus hydrolysate; (3) mixing the two hydrolysates and the remaining raw materials according to a certain ratio, and carrying out a Maillard reaction; and (4) sterilizing. According to the present invention, the prepared crab flavor seasoning sauce has characteristics of delicious and sweet odor of seafood, realistic crab flavor and mellow, delicious, thick and lingering taste, can be widely used for cooking of various types of seafood, and can be adopted as the appetizing sauce and the dish seasoning so as to provide effects of flavor increasing and fresh improving.

The invention relates to a reutilization method of oil tea shells, and belongs to the technical field of application of oil tea shells. The method includes the two processes that 1, a matrix for cultivating hypsizygus marmoreus is prepared from the oil tea shells, and the hypsizygus marmoreus is cultivated; 2, after the hypsizygus marmoreus grows to be mature and is harvested, the oil tea shells are recycled from matrix waste for cultivation, and biomass charcoal is prepared. The matrix is prepared from the oil tea shells, hypsizygus marmoreus dregs, plant ash, wheat bran and white sugar, thecultivated hypsizygus marmoreus is high in biological efficiency and high in nutritive value, meanwhile, the oil tea shells in the matrix waste are convenient to recycle, after the oil tea shells arecarbonized at different temperatures, the content of carbon, nitrogen and phosphorus of the obtained biomass charcoal is high, the alkalinity is low, and the soil fertility can be effectively improved.

The invention provides a hypsizygus marmoreus culture medium containing sawdust of pinaster and cedar. The hypsizygus marmoreus culture medium containing the sawdust of pinaster and cedar is prepared from the following raw materials in parts by weight: 35 to 57 parts of cottonseed hulls, 5 to 10 parts of bagasse, 5 to 20 parts of sawdust of pinaster or cedar, 15 to 20 parts of barley husks, 0 to 15 parts of corncobs, 4 to 5 parts of corn flour, and 2 to 3 parts of lime powder. The invention also provides a preparation method of the hypsizygus marmoreus culture medium containing the sawdust of pinaster and cedar. The preparation method comprises the steps of sequentially composting, mixing, transferring the mixed materials into a bottle, sterilizing and cooling; the composting is performed by spraying with water, stockpiling and pile-turning. The hypsizygus marmoreus output of the hypsizygus marmoreus culture medium is basically equal to or more than that of the traditional sawdust hypsizygus marmoreus culture medium; the sawdust of pinaster or cedar is used for replacing the traditional sawdust of broad-leaved trees, so that the damage to the broad-leaved tree forest can be decreased; the sawdust of pinaster is processed by the composting method, and oily aromatic hydrocarbon compounds in the sawdust of pinaster can be automatically decomposed and converted into nitrogenous fertilizer for growth of hypsizygus marmoreus during long-term stockpiling with water, and therefore, both time and force can be saved, the efficiency is high, and mass production can be carried out.

The invention discloses a method for cultivating clitocybe maxima through hypsizygus marmoreus mushroom residues. The method comprises the following steps of (1) preparing culturing materials; (2) loading the prepared culturing materials into bags, and performing sterilization; (3) performing cooling, and performing inoculation; (4) culturing mycelia; (5) preparing a mushroom producing shed; (6) opening the bags, and performing earthing up; (7) performing mushroom producing management; (8) performing harvesting; and (9) performing management after harvesting. Compared with the prior art, the method for cultivating the clitocybe maxima through the hypsizygus marmoreus mushroom residues disclosed by the invention has the advantages that the primordium appearing time can be further shortened, the yield of the clitocybe maxima can be notably increased, besides, the biotransformation rate of the clitocybe maxima can also be notably increased, and the occurrence rate of diseases and insect pests can be reduced.

The invention belongs to the field of stereo intelligent mushroom culture in a wild environment simulated original ecological environment and discloses a stereo intelligent efficient organic mushroomculture tank. A mushroom culture medium is spread on a mushroom carrier in the culture tank and supplied with nutrient solution permeating from interior of a nutrient supply cavity to the outside to realize culture of various mushrooms such as morchella esculenta, lycoperdon bovista, colorful mushrooms, colorful lotus shaped mushrooms, shiitake mushrooms, white hypsizygus marmoreus, mushrooms, coprinus comatus, needle mushrooms, red Lactarius deliciosus, pleurotus cornucopiae, agrocybe cylindracea, ganoderma lucidum, hericium erinaceus, oyster mushroom, tricholomagambosum, pleurotus nebrodensis, pleurotus eryngii, shiitake, hypsizygus marmoreus and pleurotus geesteranus. A method for culturing of various mushrooms is simple and easy to operate, convenience in large-scale industrial cultureof various mushrooms in four seasons is achieved, and supplying of various mushrooms on the market all year round is guaranteed; high yield and quality of cultured mushrooms can be realized, and highindustrial commercial value is achieved; the mushroom culture medium can be recycled for culturing the mushrooms, so that mushroom culture cost is reduced.

The present invention discloses dietary fiber enriched compound fruit and vegetable purple sweet potato fermented yogurt. The yogurt is prepared from the following raw materials in parts by weight: 200-220 parts of purple sweet potatoes, 10-12 parts of skimmed milk powder, 6-7 parts of peanuts, 4-5 parts of mung beans, 5-8 parts of arctium lappa leaves, 2-3 parts of miquel linden flowers, 4-6 parts of hypsizygus marmoreus, 7-9 parts of yacon, 3-5 parts of coconut powder, 2-4 parts of towel gourd flowers, 6-8 parts of mangosteens, 5-6 parts of pumpkin flesh, 10-12 parts of glucose, an appropriate amount of streptococcus thermophilus, and an appropriate amount of lactobacillus bulgaricus. In the production process of the purple sweet potato fermented yogurt, firstly the purple sweet potato powder is subjected to micro-porous treatment, which can enable the purple sweet potato powder to be fermented more completely, increase the yield, and at the same time, enable the obtained purple sweet potato yogurt to be delicate and even in texture, moderate in sourness and sweetness, uniform in color and luster, and appropriate in viscosity. The added peanuts, mung beans, arctium lappa leaves, etc. contain rich nutrients and the dietary fibers, and improve the nutrient structure, so that the yogurt has very good health-care efficacies.

The present invention discloses a cultivation method for hypsizygus marmoreus, and relates to the field of agricultural technology. The method comprises the following steps: (1) stirring compost; (2) bagging compost; (3) inoculating fungus bags; (4) cultivating myceliums; (5) scratching fungi and injecting water; and (6) carrying out inducement to primordium. The growth habbit of hypsizygus marmoreus is fully considered in the cultivation method, sawdust, cotton seed hulls, rice bran and corn meal are employed as main materials for cultivation, proper amount of gesso and lime are added, and pH value of the cultivation materials is adjusted to between 8 to 9, and therefore hypsizygus marmoreus is promoted to grow healthily and the yield of hypsizygus marmoreus is substantially improved.

The invention relates to the field of agricultural planting and culturing and provides a preparation and culturing method of a tree branch bacterial strain of pleurotus eryngii and hypsizygus marmoreus. The branch bacterial strain includes following raw materials, 10 kg of tree branches, 25 kg of wheat bran, 3.5 kg of gypsum powder, 0.1 kg of quick lime, 0.15 kg of saccharose, 0.01 kg of monopotassium phosphate, 0.01 kg of magnesium sulfate and 70 kg of water. Because a tree branch culture medium is good in permeability, the bacterial strain is rapid in growth of mycelia under cultivation of 23-25 DEG C, wherein the mycelia grows full in a bottle just by about 20 days, which is reduced by about 10 days when compared with common mother strain. After inoculation, the bacterial strain is germinated from multiple points at upper part, middle part and lower part, wherein the mycelia are spread from interior to exterior. Full-bottle time and full-bag time are reduced by about one week. The tree branch bacterial strain, when being used for replacing a wheat grain bacterial strain, can reach 100% in bacteria growth rate and is reduced in spreading time to 50% in a conventional method, namely, the tree branch bacterial strain just needs 25-28 days in bacteria spreading and growing while the wheat grain bacterial strain needs to 60 days to complete the bacteria spreading and growing in normal situation.