Biofertilizer with edible fungus residue as raw material and preparation thereof
A bio-fertilizer and edible fungus technology, which is applied to fertilizers, organic fertilizers, and excretion fertilizers made of biological waste, can solve the problems of insufficient technological innovation, high cost, and single function, and achieves improvement of soil aggregate structure and physical properties. , the effect of improving soil fertility
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[0062] (1) Preparation of bacterial suspension
[0063] Inoculate the Li-2013 single colony grown after streaking on the plate into the seed medium, culture at 40°C for 12 hours at 100 r / min, take 1 mL of the culture medium, centrifuge, wash twice with normal saline, and resuspend with 9 mL of physiological in salt water.
[0064] (2) Ultraviolet-Lithium Chloride-Diethyl Sulfate Compound Mutagenesis
[0065] The bacterial suspension was placed on a sterile plate, stirred and irradiated for 100s under a UV lamp with a distance of 30cm and a power of 15w. The irradiated bacteria solution was diluted and spread on the lithium chloride plate after gradient dilution, and the bacteria solution without ultraviolet irradiation was diluted and spread on the plate as a control. Wrap the uniformly coated plate above with black cloth or newspaper, incubate at 40°C for 48 hours, screen out the plate with the largest ratio of hydrolysis circle to colony diameter on the plate that grows co...
Embodiment 1
[0097] Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, and a kind of high-efficiency composite bio-organic fertilizer is provided, and the number of parts by weight becomes:
[0098] Pretreatment Crab-flavored Mushroom Slag 30, Matrine 2, Fermented Cow Dung 8;
[0099] The preparation method of pretreatment crab-flavored mushroom slag is as follows:
[0100] After cultivation, crush the slag of crab-flavored mushrooms, add crushed corncob powder and straw powder, control the moisture content at 18%, add the Aspergillus awamori culture, control the temperature at 31°C and ventilate for 15 hours, adjust the temperature at 8°C and keep it for 3 hours, then adjust the temperature To 25 ℃, spray the yeast liquid and cultivate for 15 hours.
[0101] The preparation method of fermented cow dung is as follows: comprise the steps:
[0102] Cow dung, crushed straw and crushed bitter beans are mixed according to the proportion, then ad...
Embodiment 2
[0126] A kind of high-efficiency composite biological organic fertilizer, described parts by weight becomes:
[0127] Pre-treatment crab mushroom residue 20, matrine, fermented cow dung 4;
[0128] The preparation method of pretreatment crab-flavored mushroom slag is as follows:
[0129] After cultivation, crush the slag of crab mushrooms, add crushed corn cob powder and straw powder, control the moisture content at 30%, add Aspergillus awamori culture, control the temperature at 35°C and ventilate for 10 hours, adjust the temperature at 5°C and keep it for 5 hours, then adjust the temperature To 20 ℃, spray the yeast liquid and cultivate for 10 hours.
[0130] The preparation method of fermented cow dung is as follows: comprise the steps:
[0131] Cow dung, crushed straw and crushed bitter beans are mixed according to the proportion, then add the Aspergillus niger culture to adjust the water and build a heap for fermentation. After the temperature reaches 65-70°C, keep it f...
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