930 results about "Mutagenesis" patented technology

The present disclosure provides new prime editor guide RNAs for prime editing, constructs for prime editing, and methods for using same. In addition, the present disclosure provides compositions and methods for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and / or targeted mutagenesis (e.g., insertion or deletion). The nucleotide change can include a single-nucleotide change (e.g., any transition or any transversion), an insertion of one or more nucleotides, or a deletion of one or more nucleotides. More in particular, the disclosure provides fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a prime editor RNA (PEgRNA). The prime editor guide RNA comprises an extension arm that provides a DNA synthesis template sequence which encodes a single strand DNA flap, which is homologous to an endogenous DNA sequence, but which contains the desired one or more nucleotide changes and which, following synthesis by the polymerase (e.g., reverse transcriptase), becomes incorporated into the target DNA molecule.

The invention relates to the technical field of microorganisms, and discloses burkholderia gladioli as well as a bacterial agent and application of the burkholderia gladioli. The Burkholderia gladioli (Burkholderia gladioli) CCTCC (China Center For Type Culture Collection) NO: M20242288 provided by the invention has good capacities of fixing nitrogen, dissolving phosphorus, dissolving zinc, producing siderophores and oxidizing sulfur, and can be used for regulating the nutrient structure of soil and converting substances which are difficult to utilize by plants in the soil, so that the growth of the plants is promoted, and the stress resistance of the plants is improved; the strain also can be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium, Fusarium verticillium and Rosellinia necatrix, can be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium and Rosellinia necatrix, and can also be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium, Rosellinia necatrix, Fusarium moniliforme and Rosellinia necatrix, Fusarium moniliforme and Rosellinia necatrix. Besides, the burkholderia gladioli provided by the invention is a strain separated from rape farmland soil, and compared with a strain screened by extreme environment and laboratory mutagenesis, the burkholderia gladioli has better adaptability when being applied to a planting environment, and can be stably colonized in plant rhizosphere and soil, so that the burkholderia gladioli stably plays a growth promoting role for a long time, is safe and environment-friendly, and has a wide application prospect. Wide application prospects are realized.

A wheat variety designated KS12D03-1 is disclosed, including plants and seeds thereof. KS12D03-1 is a durum wheat variety with a winter growth habit making it suitable for production in western Kansas and the High Plains. Methods for producing a wheat plant produced by crossing the variety KS12D03-1 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety KS12D03-1 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety KS12D03-1 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety KS12D03-1 and to wheat varieties or breeding lines produced by those methods are also provided.

A wheat variety designated PMWH111720248, the plants and seeds of wheat variety PMWH111720248, methods for producing a wheat plant produced by crossing the variety PMWH111720248 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety PMWH111720248 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety PMWH111720248 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety PMWH111720248 and to wheat varieties or breeding lines produced by those methods are also provided.

The invention belongs to the technical field of biological material engineering, and particularly relates to a fungal imitation leather material and a preparation method thereof. The method comprises the following steps: firstly, adopting wild fomes fomentarius subjected to 60Co irradiation mutagenesis as a production strain of a mycelium pad, and screening and optimizing components and content of a liquid culture medium to obtain a surface culture liquid culture medium suitable for the wild fomes fomentarius; secondly, by utilizing the strain and an optimized culture medium, the low-cost and large-scale production of the mycelium pad can be realized under the optimal culture condition; and the mycelium pad is subjected to various physical and chemical treatments such as plasticizing, cross-linking, hot-pressing compaction and the like, so that the mechanical strength of the mycelium pad is further improved, and the mechanical property requirements of leather products are met.

The invention discloses bacillus velezensis Scm3 and application thereof.The bacillus velezensis Scm3 is obtained by screening a strain Scm with an inhibition effect on aeromonas hydrophila from turbot intestinal contents and then conducting ultraviolet mutagenesis on the strain to obtain a strain Scm3 with an enhanced antagonism effect on aeromonas hydrophila, and the strain Scm3 is applied to the preparation of the bacillus velezensis Scm3 and the application of the bacillus velezensis Scm3 to prepare the bacillus velezensis Scm3. 16SrDNA (deoxyribonucleic acid) sequence identification and genome sequencing prove that the strain is bacillus velezensis, and the inhibition mechanism of the bacillus velezensis Scm3 on aeromonas hydrophila is researched by a non-contact co-culture method.

The invention relates to the technical field of microorganisms, and discloses trichoderma yunnanense and application thereof. The Trichoderma yunnanense CCTCC NO: M 2025645 provided by the invention is a dominant strain of a high-yield siderophore, the activity of the siderophore is 69.79%, and the Trichoderma yunnanense can be used for synthesizing and secreting carboxylic acid type siderophore; the strain has a sulfur oxidation function; in addition, the invention also provides an application of the trichoderma sp. In regulation and control of plant diseases, the trichoderma sp. Disclosed by the invention can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and the trichoderma sp. Can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae. The Trichoderma yunnanense provided by the invention is a strain separated from tobacco planting soil, and compared with a strain subjected to mutagenesis screening in an extreme environment and a laboratory, the Trichoderma yunnanense has better adaptability when being applied to a planting environment, and can be stably colonized in plant rhizosphere and soil, so that the Trichoderma yunnanense stably plays a growth promoting role for a long time, is safe and environment-friendly, and has a wide application prospect.

The present disclosure provides compositions and methods for the targeted modification of RNA molecules by RNA prime editing. The compositions and methods may be conducted invitro or in vivo within cells (e.g., human cells) for the therapeutic correction of disease-causing mutations and / or installation of motifs or mutations in RNA molecules of interest as a tool for scientific research. The disclosure provides compositions and methods for conducting RNA prime editing of a target RNA molecule (e.g., an RNA transcript) that enables the incorporation of one or more nucleotide changes and / or targeted mutagenesis of a target RNA molecule. The nucleotide change can include a single-nucleotide change, an insertion of one or more nucleotides, or a deletion of one or more nucleotides. More in particular, the disclosure provides a variety of configurations of the RNA prime editors each comprising a nucleic acid programmable RNA binding proteins (napRNAbp), such as Cas13, and an RNA-dependent RNA polymerase (RDRP), which are provided as fusion proteins or which can be separately provided in trans. The RNA prime editors are guided to a target RNA site by a guide RNA, which can be a rpegRNA that includes a template region for the synthesis of an RNA sequence to be installed on the RNA molecule attached to an available 3′ terminus. In others embodiments, the RNA template can be provided in trans.

The invention provides a microbial source anti-oxidation pigment feed additive for aquaculture and a preparation method of the microbial source anti-oxidation pigment feed additive, the microbial source anti-oxidation pigment feed additive for aquaculture is prepared by the following steps: carrying out low-temperature plasma physical mutagenesis on talaromyces albuminosus CY-G to generate a mutant strain, namely talaromyces albuminosus CY110, and then carrying out semi-solid state fermentation on the mutant strain, namely talaromyces albuminosus CY110 to produce wine red pigment. The pigment product obtained by the invention is high in yield of haematochrome, orange pigment and yellow pigment, low in citrinin content, stable in property and relatively strong in oxidation resistance, can be used as an aquaculture microorganism source anti-oxidation pigment feed additive, and has a wide application prospect. The preparation method disclosed by the invention is simple to operate, low in equipment requirement, high in production efficiency and suitable for large-scale production.

The invention relates to the field of microorganisms, and discloses an Aspergillus tubingensis strain and application thereof in preparation of meal seasonings, the Aspergillus tubingensis strain is not subjected to mutagenesis and acclimatization and has the capacity of high yield of glutaminase, feruloyl esterase, glucosidase, pectinase and protease, and when the Aspergillus tubingensis strain is applied to soy sauce brewing, the yield of soy sauce is increased. The high-value utilization of the meal raw materials can be realized, the amino acid content in the soy sauce is increased, and the flavor and nutritional value of the soy sauce are enhanced.

The invention discloses a breeding method of high-concentration cane molasses tolerant robust saccharomyces cerevisiae and high-concentration ethanol fermentation application of the high-concentration cane molasses tolerant robust saccharomyces cerevisiae, and belongs to the technical field of biology. The Saccharomyces cerevisiae NGTM-F1 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center on July 25, 2023, and the number of the Saccharomyces cerevisiae NGTM-F1 is GDMCC 63687. The Saccharomyces cerevisiae NGTM-F1 disclosed by the invention is named as Saccharomyces cerevisiae NGTM-F1. The saccharomyces cerevisiae is obtained by taking laboratory saccharomyces cerevisiae GJ08 as an original strain, carrying out ARTP (room temperature plasma) mutagenesis and adaptive evolution breeding and utilizing high-throughput screening, keeps a good growth state in cane molasses with the total sugar being 300 g / L, and has good fermentation performance in cane molasses with the total sugar being 250 g / L at the temperature of 30 DEG C. Under the condition of 30 DEG C in a conical flask, the total sugar consumed by fermentation is 250 g / L, the ethanol concentration can reach 102.30 g / L, and the fermentation efficiency is 80.8%; under the condition of 30 DEG C in a 5 L fermentation tank, the total sugar consumed by fermentation is 250 g / L, the ethanol concentration can reach 111.65 g / L, and the fermentation efficiency is 87.0%.

The invention discloses efficient carbon sequestration Brucella from an oil-containing solid waste residue source and application of the efficient carbon sequestration Brucella. The Brucella is Brucella sp A54-5 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M20241650. The Brucella is obtained through ARTP mutagenesis and screening by taking Brucella Brucella sp A54 separated from oil-containing solid waste residues of an oil field as a starting strain, has excellent characteristics of reducing pollution and sequestration carbon and relatively strong stress resistance, and has the advantages of deep recycling (soil transformation) of the oil-containing solid waste residues, improvement of the carbon sequestration capacity of petroleum-contaminated soil and improvement of the oil-containing solid waste residues. The method has a wide application prospect in the fields of petroleum-polluted soil remediation and the like, and has great significance in pollution reduction and convergence increase in petroleum and petrochemical industries under the background of supporting double carbon.

The invention discloses propionibacterium capable of obtaining high-yield propionic acid through compound mutation screening and a method for fermenting propionic acid by using the propionibacterium. The high-yield propionic acid strain YCBS010 (CGMCC No.31160) is obtained by carrying out compound mutagenesis on acid-producing propionibacterium CGMCC 1.2232 serving as an original strain through microwave-ultrasonic combined mutagenesis and compound normal-pressure and room-temperature plasma (ARTP) mutagenesis means. According to the invention, YPA15 is used as a starting strain, microwave and ultrasonic combined mutagenesis is carried out for 100 seconds to screen a bacterial strain with increased propionic acid yield, then ARTP mutagenesis is carried out for 90 seconds to screen out a high-yield mutant strain YCBS010, fermentation in a 1-ton tank verifies that the highest propionic acid yield reaches 65.87 g / L and the fermentation intensity is 0.73 g / L.h after fermentation is carried out for 90 hours, and the bacterial strain has better industrial fermentation potential.

Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and / or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named an PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incorporated into the target DNA molecule.

A wheat variety designated 6PQAN73B, the plants and seeds of wheat variety 6PQAN73B, methods for producing a wheat plant produced by crossing the variety 6PQAN73B with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety 6PQAN73B with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety 6PQAN73B are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety 6PQAN73B and to wheat varieties or breeding lines produced by those methods are also provided.

The present disclosure provides compositions and methods for the targeted modification of RNA molecules by RNA prime editing. The compositions and methods may be conducted invitro or in vivo within cells (e.g., human cells) for the therapeutic correction of disease-causing mutations and / or installation of motifs or mutations in RNA molecules of interest as a tool for scientific research. The disclosure provides compositions and methods for conducting RNA prime editing of a target RNA molecule (e.g., an RNA transcript) that enables the incorporation of one or more nucleotide changes and / or targeted mutagenesis of a target RNA molecule. The nucleotide change can include a single-nucleotide change, an insertion of one or more nucleotides, or a deletion of one or more nucleotides. More in particular, the disclosure provides a variety of configurations of the RNA prime editors each comprising a nucleic acid programmable RNA binding proteins (napRNAbp), such as Cas13, and an RNA-dependent RNA polymerase (RDRP), which are provided as fusion proteins or which can be separately provided in trans. The RNA prime editors are guided to a target RNA site by a guide RNA, which can be a rpegRNA that includes a template region for the synthesis of an RNA sequence to be installed on the RNA molecule attached to an available 3′ terminus. In others embodiments, the RNA template can be provided in trans.

The invention belongs to the technical field of microbial mutagenesis, and particularly relates to a microbial mutagenesis breeding device and application thereof. The microorganism mutation breeding device comprises a cube structure and a transmission structure, the periphery of the cube structure is wrapped with a light-proof material, and an ultraviolet lamp is arranged in the cube structure; the transmission structure comprises a single sequencing area and a mutagenesis area, and the mutagenesis area is inserted into the cube structure through the insertion opening of the cube structure and is perpendicular to the ultraviolet lamp. Microorganisms can be singly sorted through the first horizontal spiral conveying pipeline in the single sorting area and conveyed to the second horizontal spiral conveying pipeline in the mutagenesis area, so that the time and the intensity of ultraviolet irradiation of the microorganisms are uniform, the mutagenesis fatality rate of the microorganisms is remarkably reduced, the forward mutation rate is remarkably increased, and the mutagenesis rate of the microorganisms is remarkably increased. Operation is simple, workload is small, and period is short. In addition, the microorganisms are treated by using a protective agent with specific composition before single sequencing, so that the mutation uniformity and the forward mutation rate can be further improved.

The invention relates to bacillus pumilus and application thereof. The bacillus pumilus DX-fK is obtained through a chemical mutagenesis method, the bacillus pumilus DX-fK can efficiently degrade straw, the bacillus pumilus DX-fK is used as a substrate to produce feed alternative protein, and therefore the bacillus pumilus DX-fK can be applied industrially and has good industrialization prospects.

The present invention relates to a method for finding global regulators in order to induce or increase production of target secondary metabolites. The method comprises providing a bacterium indicator strain producing two fluorescent reporter signals under the same control of two different biosynthetic gene clusters (BGC), preferably highly expressed BGC, performing random mutagenesis in said bacterium indicator strain, and selecting mutant bacteria not producing said reporter signals. The invention also relates to a method to activate the production of target secondary metabolites, and to a CRISPR / Cas based single plasmid to delete or inactivate a global regulator and / or activate a BGC. Finally, the inventive method can be performed in high-throughput format allowing to speed-up the procedure.

The invention belongs to the technical field of in-vitro cultivation of calculus bovis, and particularly relates to a method for in-vitro cultivation of calculus bovis. According to the invention, firstly, the escherichia coli is subjected to bile domestication and ray induced mutation, a high-activity oxgall-resistant bezoar production engineering bacterium E.coli-NH01 is obtained through screening, a method for in-vitro cultivation of bezoar by using the engineering bacterium E.coli-NH01 is provided, the prepared bezoar meets the pharmacopoeia standard, the production efficiency of in-vitro cultivation of bezoar is improved, and the production cost is reduced. And the production cost is reduced, the overall forming period is shortened, and the key that precious traditional Chinese medicinal materials are popular can be achieved.

The invention relates to the technical field of plant stress resistance screening, in particular to a method for screening drought-resistant mutants after EMS mutagenesis of bamboo reed cluster buds, which comprises the following steps: S1, performing hydroponic seedling strengthening on wild tissue culture seedlings and EMS-treated rooting tissue culture seedlings to obtain wild bamboo reed seedlings and EMS-treated bamboo reed seedlings; s2, screening suitable concentration of PEG-6000: screening wild type bamboo reed seedlings by using a series of PEG-6000 solutions with different concentrations, comparing survival rates of the bamboo reed seedlings under different treatments, and screening suitable concentration for simulating drought stress by a PEG-6000 water culture method; s3, simulating drought stress conditions by using the screened PEG-6000 solution with proper concentration as a permeable medium, and screening the EMS treated bamboo reed seedlings to obtain drought-resistant plants. The screening method provided by the invention is not limited by seasons, has high accuracy, and is a simple, convenient and rapid method for screening drought-resistant bamboo reed plants.

The present invention belongs to the field of biotechnology, and specifically relates to a GmSGT2 gene related to plant height and branch development, and a mutant thereof and the use thereof. A Glycine max mutant having fewer branches and decreased plant height is obtained by means of EMS mutagenesis, and a target gene thereof that is located by means of a map-based cloning technique is a GmSGT2 gene of Hedou 12. It is found through searching that the gene encodes soyasapogenol B glucuronide galactosyltransferase, and can galactosylate soyasapogenol B monoglucuronide, thereby affecting the plant height of Glycine max and reducing the branches of Glycine max. Therefore, the GmSGT2 mutant can be used for cultivating dwarf high-yield Glycine max varieties, and is of great significance in the breeding of ideal plant types of Glycine max and research on important agronomic traits of plants. The mutant has broad application prospects and great research value with regard to understanding the plant height and branch regulation mechanism of Glycine max and improving the process of Glycine max breeding; and can provide an excellent germplasm resource stock for regulating the close planting and high-yield breeding of Glycine max.

The embodiment of the invention provides a method for regulating and controlling the form of ginseng adventitious roots and saponin accumulation. The method comprises the following steps: (a) carrying out mutagenesis treatment on initial ginseng adventitious roots by using ethyl methane sulfonate (EMS); (b) performing multiple rounds of screening and subculture to obtain to-be-selected ginseng adventitious roots with different forms and saponin accumulation; and (c) determining one or more target genes for regulating and controlling the form of the ginseng adventitious root and saponin accumulation based on the ginseng adventitious root to be selected.

The invention discloses corynebacterium glutamicum for producing L-isoleucine and application of the corynebacterium glutamicum. The strain is obtained by performing ultraviolet mutagenesis and structural analogue resistance screening on corynebacterium glutamicum ATCC13869. According to the method, the L-isoleucine can be produced through direct fermentation of glucose, the yield of the L-isoleucine can reach 51.69 g / L, the conversion rate can reach 28.5%, the production efficiency is improved, meanwhile, the main raw material glucose used for fermentation is low in price and easy to obtain, the method conforms to the green food safety production concept, and industrial production of the L-isoleucine is facilitated.

The present invention belongs to the technical field of biological prevention and treatment for viral diseases. Disclosed in the present invention are a tomato disease-resistant gene mutant, and the use thereof in the prevention and treatment of ToBRFV. It is found in the present invention that mutating the nucleotide at position 1927 of the coding region sequence of tomato Tm-22 gene from G to A, or mutating the amino acid at position 643 of the LRR domain of a protein that is encoded by tomato Tm-22 gene from glycine to arginine can remarkably reduce the accumulation level of ToBRFV capsid protein (CP). The gene (named Tm-22-Mut5) can serve as a novel ToBRFV resistant gene, and also retains the resistance to TMV, ToMV and ToMMV. The Tm-22-Mut5 and a pre-screened Tm-22-Mut3-1 mutant (tyrosine at position 767 of the LRR domain thereof is mutated to phenylalanine) undergo combinatorial mutagenesis to obtain a mutant Tm-22-Mut6. Analysis shows that the Tm-22-Mut6 can remarkably reduce the accumulation level of the ToBRFV capsid protein. Compared with the pFGCTm-22-Mut3-1 mutant obtained by screening in the previous research and the newly obtained mutant Tm-22-Mut5, the Tm-22-Mut6 has further improved resistance to ToBRFV.

The invention belongs to the technical field of microbial fermentation, and discloses torulopsis variabilis and application thereof. Wild torulopsis variabilis is separated from soy sauce mash, a strain of torulopsis variabilis with high yield of aspartic acid, threonine, glutamic acid, valine, methionine, isoleucine, leucine and lysine is obtained through ARTP mutagenesis, the collection number of the torulopsis variabilis ZB444 is GDMCC No: 65240, the classification name of the Latin name of the torulopsis variabilis is Wickhamiella versitis, the collection number of the torulopsis variabilis ZB444 is CGMCC No: 65240, and the collection number of the torulopsis variabilis ZB444 is CGMCC No: 65240. The strain is preserved in Guangdong Microbial Culture Collection Center on October 12, 2024. The strain is applied to soy sauce fermentation, the content of various amino acids and the content of total amino acids in soy sauce can be increased, the nutritional value of the soy sauce can be increased, and meanwhile the flavor of the soy sauce is enhanced.

The invention belongs to the technical field of microorganisms, and particularly relates to saccharomyces cerevisiae, a fungicide, application and a screening method. The invention provides a saccharomyces cerevisiae strain DXMC847, which is classified and named as saccharomyces cerevisiae, and is preserved in China Center for Type Culture Collection on November 18, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20242592. The saccharomyces cerevisiae strain BY4741 is used as an original strain, and a method of combining ultraviolet mutagenesis with flow sorting is utilized to realize high-throughput screening of acid-resistant strains, so that the acid-resistant strains can be efficiently screened, and the yield of the acid-resistant strains is improved. And finally obtaining the acid-resistant saccharomyces cerevisiae DXMC847. The result of the embodiment shows that the content of pyruvic acid, L-malic acid and succinic acid in the fermentation liquor of the saccharomyces cerevisiae DXMC847 is obviously improved compared with that of the saccharomyces cerevisiae BY4741.

The invention discloses a yellow flammulina velutipes ZJJZG002 and InDel marker with high resistance and high brittleness and application of the yellow flammulina velutipes ZJJZG002 and InDel marker, and relates to the technical field of edible mushroom cultivation, the preservation number of the yellow flammulina velutipes is CGMCC NO.41174, and the yellow flammulina velutipes is classified and named as flammulina velutipes. The strain ZJJZG002 takes yellow flammulina velutipes F24 and white flammulina velutipes W1 as parents, the parent materials are subjected to ultraviolet mutagenesis treatment and then are subjected to single spore isolation, hybridization (a hybrid combination XB51 * YU19) and fruiting tests, and the strain ZJJZG002 is not prone to umbrella opening, rich in mushroom fragrance, crisp and refreshing in taste, neat in fruiting, suitable for production in various cultivation modes and capable of injecting a new seed source for the domestic flammulina velutipes market.

The invention provides an anaerobic gas-feeding bacterium subjected to mutagenesis screening and application of the anaerobic gas-feeding bacterium in high-yield acid production through synthesis gas conversion. The anaerobic gas-feeding bacterium is a Clostridium sp. Strain P38 strain, and the preservation number of the anaerobic gas-feeding bacterium is CGMCC (China General Microbiological Culture Collection Center) No.46262. According to the invention, an acid-producing Clostridium sp. Strain P38 strain is obtained through ARTP mutagenesis screening, and through optimization of culture conditions, especially addition of sodium molybdate in a culture system and control of the pH value of the culture system, the maximum concentration of bacteria fermented by the anaerobic gas-feeding bacteria Clostridium sp. Strain P38 and the capability of fermentation acid production are improved, the maximum cell concentration of the strain can reach OD600nm-2. 0Abs, the maximum acetic acid yield is 5g / L, and the hexanoic acid content is 3.5 g / L.

The invention discloses a p-coumaric acid biosensor and application thereof, and the p-coumaric acid biosensor is characterized in that a coding gene of a CarR mutant, a Plpp1.6 promoter, a coding gene of a green fluorescent protein and a Pcar promoter mutant for controlling the expression of the green fluorescent protein are located on an expression vector pTrc99a; the biosensor provided by the invention realizes real-time sensitive monitoring of the concentration of coumaric acid by a recombinant escherichia coli strain containing the coumaric acid biosensor, can be combined with a high-throughput mutagenesis and screening system, and is beneficial to rapid and efficient screening of high-yield engineering strains of coumaric acid; and a technical guarantee is provided for production of p-coumaric acid and derivatives by efficient fermentation of microorganisms. The p-coumaric acid biosensor based on transcriptional regulatory factors is constructed, and a new strategy is provided for high-throughput screening.