The invention relates to an Epothilone analogue, a preparation method as well as a medicine composition and application thereof. Definitely, the invention relates to an analogue obtained by substituting a hydrogen in the C-9 position of Epothilone with methyl, a medicine composition as well as preparation method and application thereof, in particular to an Epothilone analogue medicine compound shown in a formula (I), a medicine composition containing effective amount of Epothilone analogue compound (I) and pharmaceutically acceptable carrier thereof and used for treating cancers or a composition of the Epothilone analogue compound and other anti-cancer medicines, a preparation method of the Epothilone analogue and application thereof in the medicines, especially the application in preparing medicaments for treating cancers, wherein the Epothilone analogue medicine compound is used for treating cancer diseases.