Novel epothilone biosynthetic gene P3 promoter as well as preparation method and application thereof

A promoter and gene expression technology, applied in the fields of biochemistry and molecular biology, can solve the problem of few promoters of epothilone biosynthesis genes, and achieve the effect of promoting wide application, promoting improvement and promoting yield
CN109679950AActive Publication Date: 2019-04-26GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
Publication Date
2019-04-26

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Abstract

The invention discloses a novel epothilone biosynthetic gene P3 promoter as well as a preparation method and application thereof. A nucleotide sequence of the P3 promoter is shown as SEQ ID NO. 1. Thepromoter of a So ce M4 epothilone biosynthetic gene is amplified by adopting a degenerate primer designing method for the first time; the promoter is subjected to cloning and promoter element analysis; and a luciferase reporter gene vector is adopted to verify the functions of the obtained epothilone biosynthetic gene promoter. The obtained novel epothilone biosynthetic gene cluster promoter canlay a molecular biological foundation for transcriptional regulation of sprangium cellulosum epothilone biosynthesis and heterologous expression of an epothilone biosynthetic gene cluster; the improvement of the yield of epothilone is promoted; and wide application of the epothilone in the aspect of biomedicines is promoted.
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Description

Technical field:

[0001] The invention belongs to the fields of biochemistry and molecular biology, and in particular relates to a novel epothilone biosynthetic gene P3 promoter and its preparation method and application. Background technique:

[0002] Sonocystis cellulosus is a myxobacteria that can produce abundant biologically active secondary metabolites. Epothilone is a sixteen-membered macrolide with broad-spectrum and high-efficiency anti-tumor activity produced by Sonocystis cellulosus class of compounds. Due to its better water solubility, lower side effects, broader antitumor activity and good antitumor activity against drug-resistant tumor cells, epothilone has become the most promising compound to replace paclitaxel. Epothilone derivatives have been approved for the clinical treatment of advanced breast cancer, but the low yield and high price of epothilone limit its wide application. The biosynthetic gene clusters of epothilones have been identified, and there ...

Claims

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