10 results about "Biosynthetic genes" patented technology

This invention discloses a *Bacillus laterosporus* strain for the stable production of nonribosomal cyclic lipolipeptide and its construction method. The M1A gene in the Bogorol biosynthetic gene cluster is knocked out using CRISPR-Cas9 technology to block the Bogorol pathway, which competes with Brevicidine for substrates. Subsequently, the pMCCas9-S48 plasmid, which integrates S8 and S41 sgRNAs and their homologous arms as repair templates, is used to simultaneously knock out S8 and S41 family peptidase genes, preventing the expression of extracellular degradation enzymes. This invention also provides the application of the above strain in the preparation of nonribosomal cyclic lipolipeptide of Brevicidine and a method for preparing the nonribosomal cyclic lipolipeptide. This strain, through reducing metabolic competition and preventing product degradation, exhibits rapid cell growth, stable production of Brevicidine, and significantly reduced degradation rate, resulting in a 66.13% increase in fermentation yield compared to the unmodified strain. This invention solves the problems of degradation and unstable yield in the production of Brevicidine, provides an effective technical approach for the industrial production of Brevicidine, and provides important technical support for the development and application of novel antimicrobial peptide drugs.

The present application belongs to the field of biotechnology, and particularly relates to a biosynthesis gene cluster of fungal polyketide compounds and application thereof. The present application provides a heterologous expression method of an aspertaichunol compound biosynthesis gene combination, and a compound aspertaichunol B is obtained through the method, and a compound aspertaichunol A is prepared through acid hydrolysis of the aspertaichunol B, which has important significance for future research and development of an efficient biosynthesis method for preparing the aspertaichunol compound.

The application belongs to the field of agricultural biotechnology and microbial engineering technology, and particularly relates to a biosynthesis gene cluster of cystobactamids and application. The nucleotide sequence of the biosynthesis gene cluster is shown as SEQ ID NO:1. The application discloses a novel biological function of cystobactamids except for antibiotics, broadens the application scene, and has a good development prospect in the field of agricultural biological control.

This invention discloses a method for heterologous production of 7-aminodeacetoxycephalosporanic acid using *Aspergillus nidulans*. This invention targets a *Aspergillus nidulans* strain that heterologously produces 7-aminodeacetoxycephalosporanic acid. Genes derived from the biosynthetic gene clusters pcbAB / C, isopentine N-acetyltransferase gene, and cefE gene, or their mutants, are assembled into a *Aspergillus nidulans* expression vector. Co-expression is performed in a *Aspergillus nidulans* basal cell strain to obtain a recombinant strain, successfully achieving heterologous expression of 7-aminodeacetoxycephalosporanic acid in filamentous fungi. Experiments show that the yield can reach 0.13 g / L at the shake-flask level. The strain constructed in this invention provides a good heterologous expression system for studying the synthesis and regulatory mechanism of 7-aminodeacetoxycephalosporanic acid.

This invention belongs to the field of synthetic biology and discloses tetracycline compounds modified with a polysaccharide group at the C8 position, their biosynthetic methods, and applications. The tetracycline compounds with a polysaccharide group modification at the C8 position provided by this invention have a novel structure; the trisaccharide and hexasaccharide groups present at the C8 position are not found in known tetracycline natural products or tetracycline skeleton antibiotics. The biosynthetic method involves using a polysaccharide group derived from *Streptomyces rubrum*. ech Gene clusters, in which mis This invention expresses the gene cluster in the *Streptomyces micranthum* host, fully utilizing the different biosynthetic enzymes contained in two similar but different tetracycline natural product biosynthetic gene clusters to synthesize novel compounds that neither host bacterium can synthesize. These compounds exhibit significant inhibitory activity against *Staphylococcus aureus*, *Enterococcus faecalis*, and *Enterococcus faecium*. This invention provides new candidate compounds for the innovative development of tetracycline-based antibacterial drugs and is of great significance to the development of microbial drug resources.

The application discloses a biosynthesis gene cluster of marine fungus pyrenophorol compounds and application thereof. The nucleotide sequence of the biosynthesis gene cluster is shown in the 1-18549th of SEQ ID NO. 1, and the biosynthesis gene cluster comprises six genes, i.e. a gene responsible for synthesis of a pyl skeleton structure, namely pylA and pylB; a gene responsible for hydroxylation of carbon 4 and 4' in the pyl, namely pylC; a gene responsible for ketone-enol tautomerism, namely pylE; a gene responsible for reduction of ketone groups at carbon 4 and 4' into hydroxyl groups, namely pylD; and a gene responsible for compound efflux, namely pylF. All the gene information related to pyl biosynthesis provided by the application can help understand the biosynthesis mechanism of the sixteen-membered ring dilactone family natural products, and provide materials and knowledge for further structural modification of the compounds.

This invention provides a filamentous fungus in which wall growth formation is suppressed, and the use of said filamentous fungus. [Solution] Use a filamentous fungus lacking one or more genes selected from the galactosaminogalactan biosynthesis gene group and one or more genes selected from the chitin biosynthesis gene group, or a filamentous fungus in which the expression of one or more genes selected from the galactosaminogalactan biosynthesis gene group and one or more genes selected from the chitin biosynthesis gene group is suppressed.

This invention belongs to the field of gene technology and discloses a bacterial strain antagonistic to konjac soft rot and its application. The *Pseudomonas pallens* strain B07 of this invention exhibits significant antagonistic activity against the pathogen causing konjac soft rot, with an inhibition zone diameter of 1.05±0.16 cm. In pot experiments, it reduced the incidence of konjac soft rot by 33.34% and the disease index by 22.22%. Simultaneously, this strain possesses plant growth-promoting characteristics such as phosphorus solubilization, siderophore production, and nitrogen fixation. It also shows broad-spectrum antagonistic activity against eight plant pathogenic fungi, including konjac white mold and *Amorphophallus bulbifer* leaf spot, with inhibition rates ranging from 44.87% to 60.03%. Whole-genome analysis shows that its genome contains 14 secondary metabolite biosynthesis gene clusters, providing the molecular basis for its antibacterial activity. This strain can be applied directly or prepared as a biocontrol agent for the prevention and control of konjac soft rot. It is environmentally friendly, residue-free, and possesses both disease prevention and growth-promoting effects, making it of significant industrial value in the green control of konjac diseases.

This invention discloses a novel antitumor macrolide compound, UCN370, its preparation method, and its applications. The structural formula of compound UCN370 is shown below. This invention successfully expressed the bacterial artificial chromosome (BAC) plasmid pUCN370, containing the UCN370 biosynthetic gene cluster, using the chassis strain *Streptomyces albus* J1074, resulting in the novel scaffold compound UCN370. The novel antitumor macrolide compound UCN370 described in this invention exhibits potent inhibitory activity against tumor cells such as HK-2 and can be used to prepare antitumor drugs.

The application belongs to the field of plant biotechnology, and specifically discloses a Punica granatum ABCG9 gene and application thereof. The PgABCG9 gene of the Punica granatum is cloned for the first time, and the nucleic acid sequence is shown as SEQ ID No. 1. The PgABCG9 is only expressed in the inner seed coat of the Punica granatum, and the transcription level is negatively correlated with the hardness of the Punica granatum seeds. Under the same growth conditions, the PgABCG9 transgenic Arabidopsis plants show slow growth, reduced lignin deposition, reduced expression level of lignin biosynthesis genes, and reduced content of metabolic products in the lignin biosynthesis pathway. Subcellular localization analysis shows that the PgABCG9 is located in the Golgi apparatus. The PgABCG9 plays a negative regulation role in the lignin biosynthesis, and can regulate the lignin synthesis of the inner seed coat of the Punica granatum, and adjust the kernel hardness, thereby laying a foundation for improving the fruit quality of the Punica granatum and other drupes from the direction of lignin in the future.