1208 results about "Ophiocordyceps sinensis" patented technology

The invention provides a group of relevant enzymes for preparing mannitol by performing anabolism on Chinese caterpillar fungus serving as a multifunctional production fungus and hirsutella sinensis based on glucose, a gene for encoding these enzymes and application thereof. The relevant enzymes include (1) hexokinase: manA1-A6 proteins of which the sequences are SEQ ID No.1-6, (2) phosphoglucoisomerase: manB1-B3 proteins of which the sequences are SEQ ID No.7-9, and (3) mannitol-1-P dehydrogenase: manC protein of which the sequence is SEQ ID No.10. In the invention, detailed researches are performed on the metabolic pathway of mannitol synthesized by using Chinese caterpillar fungus serving as a multifunctional production fungus, hirsutella sinensis and glucose on the aspect of principle, cloned DNA (Deoxyribose Nucleic Acid) comprising a nucleotide sequence provided by the invention can be transferred into engineering bacteria with transduction, conversion and conjugal transfer methods, and host mannitol is endowed with high expression by regulating the expression of a biosynthetic gene of the mannitol.

A solid state fermentation (SSF) method is provided which is effective for both small- and large-scale fungal cultivation. Also provided is SSF media for fungal cultivation. The media preferably contains a carbon source and nitrogen source to provide a carbon to nitrogen ratio of about 5:1 to about 25:1 by weight. The media may also contain a vitamin and an inorganic substance. A preferred SSF medium contains malt extract, yeast extract, peptone, glucose, water, solid base, and calcium carbonate or gypsum. Before propagating fungal mycelia in the SSF medium, the mycelia may be pre-cultivated in a solid culture medium and then in a liquid medium. Although the SSF method can be used in growing most fungi, preferred fungi include Cordyceps sinensis, Ganoderma lucidum, Antrodia camphorata, Trametes versicolor, and Agaricus blazei. The SSF method not only produces high yield of fungi, but also stimulates the production of fungal metabolites, particularly the kinds with pharmaceutical and medicinal activities. Cordyceps sinensis is preferably grown to produce the active compound H1A which is a derivative of ergosterol.

The invention provides a total artificial method for cultivating the cordyceps sinensis, and belongs to the field of the total artificial cultivation of the cordyceps sinensis. The method comprises the steps of: a. preparing a Chinese hirsutella suspension; b. acquiring, propagating and raising the host insect; c. infecting: taking the hepialus larva obtained by the step b, and soaking the suspension of the step a by a needle-shaped apparatus with the diameter which is less than 0.2mm at one end to prick the hepialus larva to complete the infection; and d. continuously raising. After the method is used, the improper mortality of the chrysalis can be reduced, and the eclosion rate of the hepialus is improved; the mating rate of the hepialus can be improved; and the infection rate of the Chinese hirsutell to the host hepialus larvae can be greatly improved, and the total artificial industrial production of the cordyceps sinensis can be realized.

The invention relates to a qualitative and quantitative analysis method for polyoses, which comprises the following steps: (1) the zymohydrolysis and the verification of the polyoses: measuring the content of a polyoses water solution by a colorimetric method; (2) comparing the change of a polyoses mapping before and after zymohydrolysis by HPSEC-ELSD analysis; (3) saccharide ingredient direct HPLC analysis of a polyoses zymohydrolysis solution or HPLC analysis after pre-column derivatization: identifying polyoses mapping and chromatogram peaks by taking a standard monosaccharose, a uronic acid, a disaccharide, and the like as contrasts and taking a chromatogram peak mass-to-charge ratio as reference, and using stable characteristic sections as a quantitative analysis index; and (4) realizing the qualitative and quantitative analysis of the polyoses by establishing polyoses zymohydrolysis responding characteristics and polyoses zymohydrolysis mapping which are based on structural information through the independent or combining application of the steps. The qualitative and quantitative analysis method can be used for the identification and quantitative measurement of the polyoses of traditional Chinese medicine, such as panax, panax pseudoginseng, gen-seng, aweto, cordyceps, ganoderma lucidum, fomes japonica, milk veteh, angelica, and the like and provides an effective method for controlling the quality of the polyoses and the products thereof.

The invention discloses a novel traditional Chinese medicine composition for treating the fatigue syndrome of sub-healthy people and the fatigue, the hypodynamia and the inferior immunologic function of a patient with acquired immune deficiency syndrome and a preparation method thereof. The main components of the traditional Chinese medicine composition comprise the following several raw materials of medicines: crude astragalus root, yam, white peony root, radix codonopsis pilosulae, angelica, solomonseal, ginseng, tuber fleeceflower, American ginseng, antler, aweto, ophiopogon root, Chinese wolfberry and prepared rehmannia root. The medicine composition can be prepared into any common oral preparation according to a conventional traditional Chinese medicine preparation method. The invention can obviously improve sub-health, fatigue syndrome, fatigue and hypodynamia of patients with acquired immune deficiency syndrome, enhances the self immunity and has the advantages of definite curative effect, obvious effect, convenient taking, quick acting, wide suitable crowd, no toxic or side effect and the like.

A dietary supplement is created, comprised of material from the following nutrients, vitamins, herbs, minerals, and food and plant substances and food and plant derivatives: lycopene, vitamin E, selenium, green tea, coenzyme Q10, garlic, folic acid, vitamin C, curcumin, seaweed, Cordyceps sinsensis mushroom, Lentinus edodes (shiitake) mushroom, and Ganoderma lucidum (reishi) mushroom. The composition is administered orally for individuals who wish to reduce their risk of disease, particularly cancer-risk.

The invention mainly utilizes different grains or forage such as coix seeds, rice, wheat, oat, etc. as the substrate of the solid state fermentation of cordyceps or ganoderma lucidum. After solid-state cultivation, fermentation production containing high polysaccharide can be gained; the fermentation production is dried and milled into powder. In addition, farina and flavoring agent can be added, then cordyceps or ganoderma lucidum coix seed powder, rice powder, wheat powder, or oat powder with rich health protection components such as dietary fiber and polysaccharide can be produced, thereby being convenient for consumers to brew and drink, or forage contained cordyceps or ganoderma lucidum for fed animals to eat can be produced.

This invention describes the large-scale fermentation and processing of Chinese caterpillar fungus. In this invention, hirsutella sinensis strains are used as the fermentation strain and cultivated in the inclined plate and shaking-bottle. 3-5% of potato, 0.5-2% of soybean powders, 0.01-0.02% of soifnum muicfun, 0.01-0.02% of Chinese yam, 0.5-2% of protein, 0.5-5% of glucose, a small amount of dipotassium hydrogen phosphorus and magnesium sulfate, and water culture medium are successively directed into all levels of fermenter for fermentation and processing. The yield of the fermented product is 20% higher than that in the present processes, the fermentation periodic time is 12 days shorter, the cost is 20% lower and the pollution rate is 2% lower. Through the DNA molecular sequence examination by the institute of microbes, Chinese Academy of Sciences, the constitution of the fermented product is the same with that of natural Chinese caterpillar fungus, and the content of cordycepin, adenosine, Chinese caterpillar fungus polyoses, sterol, biological base, amino acids and trace elements is much higher than that in natural Chinese caterpillar fungus. The pharmacological action of the fermented product is better than that of natural Chinese caterpillar fungus.

The invention provides a method of full-artificial culturing Cordyceps sinensis, including sampling, strain separating, host insect collecting, breeding and feeding, infecting and continuing feeding, on artificial condition, largely culturing Hirsutella sinensis; feeding host insects in an scale room at low altitude, completing the infection of Hirsutella sinensis to the host insects and sexuality of Cordyceps sinensis. By artificial culture, the host grubs are big and good-quality, and the success rate of infection is high, the infection time is short, the quality is stable and controllable, and the pesticide effect test fully indicates that the pesticide effect of the produced Cordyceps sinensis is equivalent to that of the natural Cordyceps sinensis and it can overcomes the disadvantages of long growing time of natural Cordyceps sinensis and low natural infection ratio, and implements the industrialized production of Cordyceps sinensis.

The invention relates to a composition for enhancing immunity and a preparation method of the composition for enhancing the immunity. The composition comprises the following raw materials: cordyceps sinensis powder, marine fish oligopeptide, corn oligopeptides, wheat oligopeptides, American ginseng extract, ganoderma extract, medlar extract and ganoderma spore powder. According to the preparation method, the cordyceps sinensis powder and three short peptide materials with biological activity are combined, and are supplemented with the plant extract capable of improving the immunity, therefore, the effect of enhancing the immunity can be achieved.

The invention provides a method for industrialized cultivation of Cordyceps militaris with high content of cordycepin. The method of the present invention uses the screened bacterial strains Cordyceps militaris COB201 and CGMCC No.1823 to carry out optimized cultivation to obtain Cordycepin militaris with a high content of cordycepin. The content of cordycepin in the product reaches 1.7% after testing. The method of the present invention is simple and suitable for large-scale industrialization Production.

The invention belongs to the field of biofermentation engineering. A pharmaceutical medium and a fungal strain which have blood sugar lowering efficacy are used as bidirectional fermentation raw materials, wherein the pharmaceutical medium is mainly composed of the following raw materials: cornel, gynostemma, momordica grosvenori, winged euony twigs, balsam pear and polyrhachis vicina; the fungalstrain is composed of the following strains: inonotus obliquus, cordyceps, phellinus igniarius, polystictus versicolor, and grifola frondosa; and bi-directional multi-fungi fermentation is carried out between multiple edible and pharmaceutical fungi and Chinese herbal medicines by a bidirectional fermentation process, thus organisms in two kingdoms or three kingdoms are organically combined to obtain an entirely new blood-sugar-lowering pharmaceutical mycoplasm product. The obtained product can generate 1+1>2 physiological function efficiency for hypoimmunity, has the actions of obviously lowering blood sugar but not increasing insulin concentration, has significant positive effects on helping treating and controlling diabetic complications, and can be used as an oral medicament for preventing and treating diabetes or a health-care product for adjusting blood sugar.

The present invention discloses one kind of health Chinese medicine wine. The health Chinese medicine wine is prepared through soaking 24 kinds of Chinese medicinal materials, including ant, wolfberry fruit, fleeceflower root, prepared rhizome of rehmannia, Chinese yam, etc. in grain spirit. It has the functions of tonifying heart, invigorating Qi and blood, warming kidney, invigorating Yang, raising immunity, etc.

The invention relates to tea drink with main materials of purple potato leaves and blueberry leaves. The tea drink is precisely prepared by inactivating, kneading and drying 10-50 parts by weight of purple potato leaves, 10-50 parts by weight of blueberry leaves, and the materials of purple potato flower, purple potato stem, blueberry flower and the natural material of cordyceps militaris, and thelike. The purple potato leaves, blueberry leaves, purple potato flower, purple potato stem, blueberry flower and the natural material of cordyceps militaris are rich in vitamin C. The anthocyanin ingredients contained in the blueberry leaves can delay aging and improve the immunity of a human body. The tea drink has obvious effect and favorable stability without toxicity. After drunk by most people, the tea drink is proved to have rich nutrition, comfortable mouth feeling, and convenient drinking and be easily absorbed. The tea drink has the characteristics of specific prescription, reasonable formula, simple process, strong health-care function, safety and non-side effect, is good for both children and adults, can be drunk for a long time and has good health-care effect.

The invention discloses a culture medium to ferment and manufacture Cordyceps sinensis mycelium, which consists of bevel culture medium and fermentation culture medium, wherein different culture mediums are adopted in different stages to ensure plain growth of the Cordyceps sinensis and equal quality of fermented and natural Cordyceps sinensis, which modifies the product quality and improves the production with certain effect. The invention supports the character of nourishment with natural Cordyceps sinensis, which is fit for industrial manufacturing need.

A manufacturing method for cordyceps sinensis (Berk.) sacc polysaccharide relates to the technical field of food microorganism application, and comprises the following steps: weighing raw materials, wherein rice bran is 1-3 g per 100 ml, bran is 1-3 g per 100 ml, potassium dihydrogen phosphate is 0.09-0.18 g per 100 ml, and magnesium sulphate is 0.04-0.08 g per 100 ml; material filling with natural pH after adding required water, and sterilizing and cooking at the temperature of 121 DEG C for 30 min; inoculating cordyceps sinensis (Berk.) sacc CCTCCM2013285 liquid seed after cooling, wherein the inoculation amount is 8-10%, the fermentation temperature is 23-28 DEG C, and the fermentation time is 4-7 d; respectively obtaining exopolysaccharides and mycelia polysaccharide after conducting deproteinization, alcohol precipitation, centrifugal separation, and vacuum drying on centrifugal separation liquid extracted through breaking cell wall hot water of fermentation material centrifugal separation liquid and hypha. The method better realizes the purpose that rice bran and bran are efficiently and high-valuedly transformed to cordyceps sinensis (Berk.) sacc polysaccharide after being subjected to liquid state fermentation.

A technique for culturing cordyceps by insect includes such steps as collecting fresh wild cordyceps, tissue separation to obtain its bacterial strain, reproduction, culturing slant strain and liquidbacteria, choosing host insect or host insect surrogate, spraying bacterial liquid for infecting the host insect, and culturing in the condition stimulating the wild environment. Its advantages are low cost and high effect.

The invention discloses a corayceps militaris industrial cultivation method. The corayceps militaris is cultivated in an industrial way by strain preparation, blending, bottling, sterilizing, inoculation, culture, germination and collection; fimilar artificial substitute materials are used as the culture materials, which reduce manufacture cost. In addition, the corayceps militaris obtained by the invention has the characteristics of good quality stability and high biological percent conversion, etc.

The invention discloses a method for extracting and purifying polysaccharide from a fermented mycelium of Cordyceps sinensis. The method comprises the following steps of: using the fermented mycelium of Cordyceps sinensis as a raw material to produce crude polysaccharide of the Cordyceps sinensis via degreasing, aqueous extraction, concentration and alcohol precipitation; then, redissolving the crude polysaccharide into distilled water, deproteinizing the crude polysaccharide by Sevag, decoloring the deproteinized crude polysaccharide by H2O2, dialyzing the decolored crude polysaccharide, and precipitating the dialyzed crude polysaccharide by alcohol to produce primarily purified polysaccharide; finally, purifying the primarily purified polysaccharide by ion-exchange chromatography, collecting different produced compositions stepwisely, dialyzing the compositions, precipitating the dialyzed compositions by alcohol, drying the precipitated compositions to produce the polysaccharide of the Cordyceps sinensis after the precipitated compositions are orderly washed by absolute ethyl alcohol, acetone, aether and so on. In the produced polysaccharide of the Cordyceps sinensis, MPS2 is identified to be homogeneous polysaccharide by ultraviolet absorption spectrum and gel chromatography, with high content of total sugar. The method has the advantages of simple and convenient extraction steps and low cost.

A functional health-care beverage for elongating life of man and taking care of the health of man is prepared from the grains wine and 43 Chinese-medicinal materials including ginseng, pilose antler, genoderma, cordyceps, etc.

The invention provides a method for increasing the yield of natural cordyceps sinensis. The method comprises the following steps of (1) selecting a base for increasing the yield of natural cordyceps sinensis; (2) placing a trapping lamp; (3) preparing trapping liquid; (4) screening and preparing culture medium of hepialidae hirsutella sinensis and paecilomyces hepialid; and (5) controlling yield increase conditions. According to the method, at the adult stage, a high pressure mercury vapor lamp is used for trapping, so the imagoes of hepialus armoricanus around the lamp fly to the base, and the egg-laying amount is increased; ethanol, vinegar and sugar solution are sprayed on meadows beneficial to larva growth, so the larvae gather together; and the hepialidae hirsutella sinensis and the liquefied paecilomyces hepialid are sprayed on the gathering area of larvae to raise the infection rate of the larvae of hepialus armoricanus, so the aim of increasing the yield of cordyceps sinensis is achieved and the industrialized production is realized.

The invention relates to a fermented product which is obtained by fermenting valuable medicinal edible fungi, especially aweto, and a preparation method thereof. Raw materials for preparing the active component of the product comprises 1-2 loops of aweto strains or aweto, sea buckthorn, wild peaches, mulberries, dark plums, oranges, etc. The preparation method comprises the following steps of: culture preparation; aweto ferment raw juice preparation; fruit ferment raw juice preparation; probiotics fermentation. The beneficial effect of the invention is that: functional components are accumulated by aweto fermentation so as to achieve the purposes of multi-efficacy of aweto of lung invigoration and kidney nourishment, immunoenhancement, antioxidation, radiation resistance, and the like.

The invention discloses a method for culturing cordyceps militaris bacterium. The method is as follows: finished bacterium powder is obtained after natural cordyceps militaris is subjected to separation and purification, culturing on a test tube slant, culturing of liquid first-grade seeds and second-grade seeds in oscillators, culturing of small-size seeds in a seed fermenter, concentration and spray-drying. The method for culturing cordyceps militaris bacterium disclosed by the invention has the beneficial effects that: the yield of cordyceps militaris can be increased and the price of cordyceps militaris can be reduced fundamentally; and the method plays a role in relieving pain of all peoples suffering from diseases and makes a wonder for traditional Chinese medicine treasure.

The invention discloses an anti-cancer medicinal composition, which comprises 15-70 parts by weight of plant polysaccharide and mainly comprises the following medicinal crops or mainly comprises a Chinese medicinal composition prepared by the following medicinal crops in parts by weight: 18-22 parts of astragalus, 13-17 parts of ginseng, 22-26 parts of angelica, 13-17 parts of honeysuckle and 13-17 parts of root of straight ladybell, wherein the plant polysaccharide is selected from one more of ganoderma lucidum polysaccharide, lentinan polysaccharide, cordyceps sinensis polysaccharide, coriolus versciclor polysaccharide, agaricus blazei polysaccharide, hericium erinaceus polysaccharide, maitake polysaccharide, ginkgo polysaccharide and ginseng polysaccharide. The invention also disclosesthe Chinese medicinal composition in the anti-cancer medicinal composition. The anti-cancer medicinal composition provided by the invention is used for treating advanced cancer in the case that surgery and concurrent chemoradiotherapy are unsuitable or invalid, particularly has an obvious treatment effect on the advanced gastric cancer; the total effectiveness ratio reaches about 90 percent; the total cure rate reaches about 30 percent; the three-year survival rate reaches about 40 percent; and the five-year survival rate reaches about 25 percent.

A method for artificially culturing north cordyceps to increase the contents of its physiological active components (cordyceptic acid, cordycepin and cordyceptic polyose) includes such steps as washing fresh wild north cordyceps, section, regulating pH of culture medium to 6-7, sterilizing, shaping slant, slant culturing at 20-25 deg.C for 7-10 days, regulating pH of liquid culture medium to 6-7,sterilizing, inoculating, culturing, preparing solid culture medium from rice and sorghum grains, inoculating, and culturing at 25-29 deg.C for 40-60 days.

The invention relates to a cordyceps militaris fruit body culture medium, mainly comprising a starch substrate and inorganic salt containing phosphorus, potassium and magnesium ions. The cordyceps militaris fruit body culture medium is characterized by further comprising cordyceps fungi powder which is powder containing cordyceps militaris or powder containing cordyceps militaris mycelium. The invention also provides a preparation method of the cordyceps militaris fruit body culture medium. The raw material of zhao cordyceps fungi powder as the important components of the culture medium is cultured waste material, namely culture medium after the cordycepin is cultured or waste material of the fruit body, and therefore, the production cost is reduced. The cordyceps militaris fruit body cultured by using the culture medium, the grown fruit body has excellent quality, and the content of adenosine and cordycepin as effective components is obviously improved compared with the common culturemedium.

The invention discloses rhodiola crenulata essence with skin softening and anti-wrinkle effects. The rhodiola crenulata essence is mainly prepared from rhodiola crenulata extract, ganoderma lucidum extract, cordyceps sinensis extract, saffron extract, saussurea involucrata extract and corresponding adjuvants. The rhodiola crenulata essence, which is prepared by effectively compounding the natural-activity extracts of rhodiola crenulata, ganoderma lucidum, cordyceps sinensis, saffron, saussurea involucrata and the like together and mixing with the adjuvants, can promote skin metabolism, enhance activity, eliminate injuries caused by oxygen free radicals, improve the skin elasticity, enable the skin to keep tight, young and smooth, and realize effects of excellent deep-moisturizing, whitening, freckle removing and skin softening, thus slowing down the aging of organisms.

The invention supplies a high activity wild Chinese caterpillar fungus strain and the artificial planting method. The Chinese caterpillar fungus strain is GDIM_C050423 CCTCC M 206051 that could produce fruiting body with high activity material. The method includes mother strain making, first level liquid strain making, second level liquid strain making, inoculating, artificial plating and gathering. The fruiting body has the feature of rod shaped, no branch or only has short branches at the top, and tip shaped top. It has abundant nutritive value and health protection function. It contains over 39.2% cordycepic acid.

An antitussive cream for treating chronic tracheitis, infantal pneumonia, cough, etc. is prepared from 18 Chinese medicinal materials including cordyceps, gecko, ginseng, tangerine peel, etc. throughpretreating of raw materials, mixing them, decocting and shaping. Its advantage is high curative effect.

The invention belongs to the technical field of fungus culture, particularly to a method for culturing mycelia and sporocarps of aweto, which solves the problem that artificial aweto in the prior art can not be cultured in a large scale. The method comprises the following steps of: selecting cephalosporium acremonium and Isaria cicadae; preparing a cephalosporium acremonium liquid strain and an Isaria cicadae liquid strain; preparing a solid culture medium from corn grit, soybean flour, wheat grains, sorghum grains, silkworm chrysalis meal, whole chicken eggs, black bean coarse powder containing hulls, degreased linseed oil and a nutrient solution, wherein the nutrient solution comprises chicken blood, pine needles, carrots, black beans and cattle bone; evenly mixing, and inoculating; and culturing to obtain the sporocarps and the mycelia. The detection indicates that the sporocarps contain at least 0.02% of adenosine and at least 0.1% of cordycepin, and the mycelia contain at least 0.055% of adenosine; the finger-print test result indicates that the degree of similarity of the sporophores and the mycelia is greater than 97%; and acute toxicity experiments and long-term toxicity experiments indicate that the sporophores and the mycelia have no toxicity and can be used as substitutes of natural aweto.