The invention discloses a
seedling raising method of
plateau rhodiola crenulata, comprising the following steps of: firstly, preparing a
seedling source by the tube culture of
seedling, soaking a seed for 12-24h, wherein the seed is soaked in
ethanol for 30 plus / minus 5s firstly, and then soaked in HgCl for 25-30min; secondly, washing with
sterile water 3-5 times, and sipping up water on the surface of an explant for later use; thirdly, under the sterile condition, inoculating on an Ms culture medium, transferring onto 1 / 2 Ms culture medium after seed
germination, simulating a wild environment, controlling a day and night
temperature difference, inducing formation of a lignified root, and
transplanting seedling in a matrix from a
bottle after seedling
adaptation; fourthly, field planting in the field about 6 months after
plantlet transplanting, and harvesting about 24 months. The invention is mainly suitable for a seedling raising method for planting the
rhodiola crenulata in a
plateau area such as Tibet and the like without adopting
hormone treatment, which takes the seed as an explant material and induces the lignified root by regulating a
temperature difference and illumination of a culture room and sterile culture, and has the advantages of rapid reproductive speed and good hereditary.